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GeneArt Engineered Cell Models
The new workhorse cell lines for genome editing
The world’s largest collection of engineered cell lines,
great for rapid hypothesis testing
Get instant access to over 14,000 validated, ready-to-go
cell lines engineered using CRISPR-Cas9 technology. The
Invitrogen™ GeneArt™ Engineered Cell Models collection
offers a superior resource for functional characterization
of genes and their role in cellular processes, signaling
pathways, disease development and progression, and drug
response. These cell lines are validated with defined genome
modifications, on the shelf and ready to ship. Traditional cellbased assays compare patient cells with cells from healthy
individuals, which can differ genetically in thousands of
ways. GeneArt Engineered Cell Models offer a system where
the control and mutant cell lines differ only by the mutation
you wish to test, enabling greater confidence in your
results. Now you can transition from genomic information
to functional characterization faster than ever before—
accelerate your research with the new workhorse cell lines
for genome editing.
GeneArt Engineered Cell Models are:
•Affordable—academic pricing is comparable to patientderived cell lines
•Verified—extensive quality control
•Comprehensive—the single largest bank of isogenic cell
lines with over 14,000 available
•Proven—used in hundreds of research labs and published
in top scientific journals
•Available—catalog cell lines can be delivered within
one week
Haploid cellular models represent an attractive alternative
for in vitro genetic analyses of molecular, cellular, and
developmental events. Haploid cellular models are easier to
generate than modified diploid cell lines, as only one allele
needs to be targeted to obtain a complete loss-of-function
phenotype.
The HAP1 cell line is a near-haploid human cell line that
allows rapid gene editing to be performed. HAP1 cells are
adherent with fibroblast-like morphology (Figure 1). These
cells are ideal for applications where a workhorse cell line
is needed for rapid hypothesis testing, similar to the way
HEK 293 and HeLa cells have been used to accelerate
research programs for years. The GeneArt Engineered Cell
Models collection includes knockout cell lines generated
using HAP1 cells and a wide range of other cell lines.
Diploid
Haploid
Mutation masked
by second copy
Mutation leads
to knockout
•Controlled—each cell line comes with a matched parental
cell line
HAP1 is the workhorse cell line for genome
editing experiments
Knockout cell lines are the most powerful in vitro models
available for understanding gene function. One of the
challenges to the rapid generation of knockout cell lines in
mammalian cell models is the diploid nature of the genome,
which requires mutations that eliminate gene function
to be introduced into both copies of the gene to ensure
complete knockout.
Figure 1
Figure 1. Spectral karyotyping and brightfield imaging of HAP1 cells. HAP1 cells have defined copy number and unambiguous genotyping.
Confidence in your results
GeneArt Engineered Cell Models
consist of a clonal cell line bearing your
mutation of choice, which has been
verified by Sanger sequencing. All
GeneArt Engineered Cell Models ship
with the unedited, isogenic parental cell
line, providing the perfectly matched
control to enable confidence in
your results.
Wild type cell line
Single-cell
dilution
Cas9
CRISPR-Cas9
genome editing
Pre-engineering
cell line validation
• SNP Array 6.0
• Sanger sequencing
• Digital PCR
• RT-PCR
Post-engineering
cell line validation
Wild type cell line
Clonal mutant cell line
Isogenic pair of cell lines
Clonal mutant cell line
Figure 2. GeneArt Engineered Cell Models undergo extensive characterization before and
after gene editing. When appropriate, the originating wild type cell line can be diluted to a single cell
ensure a clonal population exists prior to gene editing. This clone goes through cell line validation
The isogenically matched cell pairs that
Figureto 3
and if successful is taken forward to gene editing. The resulting clonal mutant cell line goes through
you receive eliminate the background
the same rigorous cell line validation process.
Figure 4
genetic variation observed with other
cell models and provide you with
greater confidence that any differences
observed between the mutant and the
parental cell line are due to your specific
mutation of interest. All of our cell lines
undergo extensive QC and validation
(Figure 2).
Genome modulation and engineering services
Looking for something more custom? We can help you from start to finish.
For more information, contact us at
[email protected]
Accelerate your research and search for your cell line at
thermofisher.com/engineeredcells
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