Download Homework Assignment #7

Homework 7
For Problem 1 (a) through (d), focus on each question separately. Read all four and before answering any and try to
identify how they relate to each other but also how they are distinct.
1a) What are HbA and HbS and how do they differ from each other? Focus on the molecules! (10 Points)
1b) How does the difference between HbA and HbS relate to whether someone does or does not haveSickle-cell trait or
Sickle-cell disease? (10 Points)
1c) What are HbA and HbS (note the italics!) and how do they differ from each other? Focus on the molecules! (10
Points)
1d) How does the difference between HbA ad HbS relate to whether someone does or does not have Sickle-cell trait or
Sickle-cell disease? (10 Points)
Problem 2 (10 Points)
The table below lists partial information for the DNA, mRNA codon, tRNA anticodon and polypeptide amino acid
sequence for a portion of a protein coding gene. Use the information provided and the genetic code on page 360 and
on the wiki site http://en.wikipedia.org/wiki/Genetic_code#Start.2Fstop_codons to fill in the missing information. A
second copy of the table is provided in case you need it.
nontemplate strand 5’ to 3’
___ A__ ___
template strand (3’ to 5’)
___ _A_ ___
mRNA codons (5’ to 3’)
__C ___ ___
tRNA anticodons (3’ to 5’)
___ __U __A
Three-letter amino acid code ___ ___
cys
Protein
Single-letter amino acid code
N
_
_
(second copy of the same table below just in case you need it)
___
__T
___
___
___
E
GGC
___
___
___
___
_
___
___
___
GUC
___
_
__T
___
_A_
U__
___
_
__A
___
___
___
glu
_
__T
_C_
___
U__
___
_
nontemplate strand 5’ to 3’
template strand (3’ to 5’)
mRNA codons (5’ to 3’)
tRNA anticodons (3’ to 5’)
Three-letter amino acid code
Protein
Single-letter amino acid code
___
__T
___
___
___
E
GGC
___
___
___
___
_
___
___
___
GUC
___
_
__T
___
_A_
U__
___
_
__A
___
___
___
glu
_
__T
_C_
___
U__
___
_
DNA
DNA
___
___
__C
___
___
N
A__
_A_
___
__U
___
_
___
___
___
__A
cys
_
Problem 3 (10 Points) The lines below represent single strands of DNA in a double-stranded DNA molecule. The ends of
the top strand are labeled. Use the lines to illustrate a eukaryotic gene that has two introns. Include the following in
your drawing: promoter, transcription start site, all exons, both introns, the 5’ and 3’ splice site of the introns, a
reasonable location for the ATG start codon and a TAA stop codon, the location of the polyadenylation signal, the
identity of the template and nontemplate DNA strands.
tacatttgcttctgacacaactgtgttcactagcaacctcaaacagacaccatggtgcatctgactcctgaggagaagtctgccg
ttactgccctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcaggttggtatcaaggttacaagacaggtt
taaggagaccaatagaaactgggcatgtggagacagagaagactcttgggtttctgataggcactgactctctctgcctattggt
ctattttcccacccttaggctgctggtggtctacccttggacccagaggttctttgagtcctttggggatctgtccactcctgat
gctgttatgggcaaccctaaggtgaaggctcatggcaagaaagtgctcggtgcctttagtgatggcctggctcacctggacaacc
tcaagggcacctttgccacactgagtgagctgcactgtgacaagctgcacgtggatcctgagaacttcagggtgagtctatggga
cgcttgatgttttctttccccttcttttctatggttaagttcatgtcataggaaggggataagtaacagggtacagtttagaatg
ggaaacagacgaatgattgcatcagtgtggaagtctcaggatcgttttagtttcttttatttgctgttcataacaattgttttct
tttgtttaattcttgctttctttttttttcttctccgcaatttttactattatacttaatgccttaacattgtgtataacaaaag
gaaatatctctgagatacattaagtaacttaaaaaaaaactttacacagtctgcctagtacattactatttggaatatatgtgtg
cttatttgcatattcataatctccctactttattttcttttatttttaattgatacataatcattatacatatttatgggttaaa
gtgtaatgttttaatatgtgtacacatattgaccaaatcagggtaattttgcatttgtaattttaaaaaatgctttcttctttta
atatacttttttgtttatcttatttctaatactttccctaatctctttctttcagggcaataatgatacaatgtatcatgcctct
ttgcaccattctaaagaataacagtgataatttctgggttaaggcaatagcaatatctctgcatataaatatttctgcatataaa
ttgtaactgatgtaagaggtttcatattgctaatagcagctacaatccagctaccattctgcttttattttatggttgggataag
gctggattattctgagtccaagctaggcccttttgctaatcatgttcatacctcttatcttcctcccacagctcctgggcaacgt
gctggtctgtgtgctggcccatcactttggcaaagaattcaccccaccagtgcaggctgcctatcagaaagtggtggctggtgtg
gctaatgccctggcccacaagtatcactaagctcgctttcttgctgtccaatttctattaaaggttcctttgttccctaagtcca
actactaaactgggggatattatgaagggccttgagcatctggattctgcctaataaaaaacatttattttcattg
Problem 4. The sequence of one strand of the transcribed region of a human beta
globin gene is shown above. The sequence reads 5’ to 3’ from left to right and top to
bottom. The sequence includes 3 exons and two introns. The 1st exon begins at the 5’
end of the sequence and the 3rd exon ends at the 3’ end of the sequence. The start and
stop codons are underlined. The beta globin polypeptide sequence is shown below.
mvhltpeeks avtalwgkvn vdevggealg rllvvypwtq rffesfgdls tpdavmgnpk vkahgkkvlg afsdglahld
nlkgtfatls elhcdklhvd penfrllgnv lvcvlahhfg keftppvqaa yqkvvagvan alahkyh
a) Use the sequence to identify all beta globin codons (10 Points)
b) Draw a box around the exons. (10 Points)
c) Does this sequence contain a match to the Kozak consensus sequence?
3 sentences should do it.) (10 Points)
Explain (2 or