Download Eukaryotic Gene Regulation Exercise - KEY

Eukaryotic Gene Regulation Exercise - KEY
Selected Answers: Data Set 1: Question 1: Eukaryotic genes can be regulated by DNA regions far away from the gene. Data Set 2: Question 1: Some physical interaction with the DNA disrupts transcription at some promoters in eukaryotes. Check for Understanding Questions: 1. Histone deacetylation would be expected to increase association of histones and DNA, since the positively charged histones would interact tightly with the negatively charged DNA. 2. Transcription, DNA replication, mitosis/meiosis Data Set 3: Question 1: In eukaryotes, interior regions of the mRNA have been removed and something has been added to the ends of the mRNA Check for Understanding Questions: 1. Transcription and translation occur in separate compartments in eukaryotic cells. In prokaryotes transcription and translation are coupled. 2. Data Set 4: Question 1: Lysine amino-­‐acids are somehow important for regulating protein life-­‐span Check for Understanding Questions: 1. Not necessarily, since some lysines are not targets for ubiquitin modification 2. The proteins that are not regulated by ubiquitination would be unaffected The proteins that are normally regulated by ubiquitination would have longer life-­‐spans Integration Questions: 1. To make RNA 2. It is more efficient energetically and for resource use. It saves space and ensures that organisms can respond to environments appropriately. 3. Type of Regulation or Found in prokaryotes Found in eukaryotes Processing (Yes or No) (Yes or No) Repressor binding Yes Yes Activator b
inding Yes Yes Alternative splicing No Yes Histone acetylation No Yes Ubiquitination No Yes 4. Spliced mRNA differently… Some genes "loosened", others not… Some proteins targeted for degradation 5. A. Before transcription: Histone acetylation Molecule affected: DNA B. After transcription but before translation: Splicing Molecule affected: mRNA C. After translation: Ubiquitination Molecule affected: protein