Download Second test Spr 02

Biochemistry 694:301
Second Exam, Dr. Deis
Wednesday Mar. 13, 2002
Name___________________________
Last 5 digits of I.D. Num ___________
Row Letter ____ Seat Number ______
This exam consists of two parts. Part I is multiple choice. Each of these 25 questions is
worth two points. Answer the Part I questions on this sheet, below. Answer the Part II
questions on the question pages.
Please use BLOCK CAPITAL letters like this --- A, B, C, D, E. Not lowercase!
1. ______
10. ______
18. ______
2. ______
11. ______
19. ______
3. ______
12. ______
20. ______
4. ______
13. ______
21. ______
5. ______
14. ______
22. ______
6. ______
15. ______
23. ______
7. ______
16. ______
24. ______
8. ______
17. ______
25. ______
9. ______
GRADE:
Part I Total
_______
Part II:
II-1
II-2
II-3
II-4
II-5
_______
_______
_______
_______
_______
Part II Total
_______
Total, I & II
_______
694:301 Second Exam, page 2
Name ________________________
1.
Hemoglobin's binding is affected by a variety of heterotropic effects. Which of
the following is not a negative allosteric modulator?
A. H+
D. 2,3 BPG
B. CO2
E. none of the above
C. oxygen
2.
If a solution of Hemoglobin is oxygenated to half its capacity, what would you
expect to find in the solution?
A. only Hb(O2)2
D. mono, di, and trioxy Hb
B. half Hb, half Hb(O2)4
E. none of the above
C. equal amounts of Hb, Hb(O2)2, and Hb(O2)4
3.
The heme group is positioned in the heme pocket by Fe binding to a residue of
A. histidine
D. histamine
B. alanine
E. glutamine
C. serine
4.
Where does 2,3 Bisphosphoglycerate bind to the Hemoglobin molecule?
A. it binds to the heme iron
D. at the opening of heme pocket
B. to the COOH end of alpha chain E. at the center of the tetramer
C. The E helices
5.
The 3-epimer of D-Glucose would be
A. Fructose
D. Allose
B. Galactose
E. Cellobiose
C. Mannose
6.
Lab scientists have synthesized a form of Maltose, with an alpha 1 – alpha 1
glycoside instead of the normal alpha 1-4 connection. Is this a reducing sugar?
A. yes
B. no
7.*
Identify the "chair" structure at right:
A. α-D-mannose
D. β-D-galactose
B. β-D-mannose
E. α-D-glucose
C. α-D-galactose
F. β-D-glucose
8.
Milk sugar, or Lactose, contains what sort of glycosidic linkage?
A. α 1-3
D. β 1-4
B. β 1-3
E. α 1-6
C. α 1-4
F. β 1-6
9.
The structure of sphingosine (with nothing attached to it) does NOT include
what?
A. 18 carbons
D. one amino group
B. 2 hydroxyls
E. one double bond
C. 1 carboxyl group
694:301 Second Exam, page 3
10.
Membranes are NOT generally
A. asymmetrical
B. electrically polarized
C. made of lipids and proteins
Name ________________________
D. covalent assemblies
E. bilayers
11.
Which would have a higher melting point?
A. a saturated fat like Tristearin (no d.b.)
B. an unsaturated fat like Triolein (one double bond)
C. there's no way to tell
12.*
The polar lipid shown below is
A. phosphatidyl serine
B. phosphatidyl choline
C. phosphatidyl ethanolamine
D. phosphatidyl inositol
E. none of the above
13.
Potassium can not pass through the voltage gated Sodium Channel because
A. inactivation prevents it
D. K+ is too large
B. too much Sodium gets in way
E. none of the above
C. K+ is too positive
14.
The Acetylcholine Receptor is composed of how many subunits?
A. 4
D. 8
B. 5
E. 12
C. 6
15.
The Multidrug Resistance Protein (MDR)
A. pumps out small molecules
B. is an ABC transporter
C. has some homology with CFTR, the Cystic Fibrosis Transmem. Reg.
D. all of the above
16.
Acetylcholinesterase has a turnover number (k2) of about
A. a billion per second
D. 250 per minute
B. 25,000 per second
E. none of the above
C. 250 per second
17.
In "standard" conditions, [ATP] = 1 Molar, [ADP] = 1 Molar, and [AMP] = 1
Molar. What's the value of Atkinson's Energy Charge?
A. 1.0
D. 0.5
B. 0.9
E. 0.33
C. 0.67
F. - 1.0
18.
Krebs' diagram of the three stages of Catabolism included the Citric Acid Cycle
as
A. Stage I
D. Stage IV
B. Stage II
E. none of the above
C. Stage III
694:301 Second Exam, page 4
Name ________________________
19.
What factors explain the high energy of ATP compared with a sugar phosphate?
Choose two answers.
A. Glycosides
D. Electron transport
B. Entropy
E. Resonance Stabilization
C. Charge Repulsion
20.
One of the enzymes of Fatty Acid Synthesis is named 2,4 Dienoyl CoA
Reductase. Based solely on your knowledge of cofactors and how they work, you
should be able to predict that the cofactor for this enzyme is:
A. FADH2
D. CoASH
B. NADPH
E. Thiamine Pyrophosphate
C. NADH
21.
How many Calcium ions (maximum) can Calmodulin bind?
A. 2
D. 6
B. 3
E. 12
C. 4
22.
One of the second messengers in Protein Kinase C activation is strongly
hydrophobic. It is often known by its abbreviation
A. PIP2
D. SRO
B. CPU
E. DAG
C. IP3
23.
Protein Kinase A phosphorylates enzymes on what kind of residue?
A. Tyr
D. Ala
B. Ser
E. Cys
C. His
24.
Which was described in a handout as "stupid"? (this question counts)
A. Lemur
D. Chambered Nautilus
B. Octopus
E. Fish
C. Number Five
25.
After this test I will
A. laugh
B. cry
C. drink
D. watch Sponge Bob
E. cannot tell from data provided
694:301 Second Exam, page 5
PART II
1.*
Name ________________________
Answer these questions here on the question pages.
a.
On the axes provided, draw the loading curves for hemoglobin and
myoglobin and label which is which. Indicate partial pressure in torr and show
where P50 occurs for each curve.
(4)
b.
Sketch the heme group or a tetrapyrrole ring
(2)
c.
The beta chain of HbA starts with the amino acid sequence VHLTPEEK.
How is this changed in HbS? What goes wrong, on the molecular level, during a
sickling attack? Describe the effects of the disease as shown in class.
(4)
694:301 Second Exam, page 6
2.
Name ________________________
a.
Draw the disaccharide D-mannopyranosyl α1-4 β D-xylulofuranose in the
Haworth projection. For partial credit just draw D-Mannose and D-Xylulose in
the Fischer projection.
(4)
b.
Draw the alpha and beta anomers of D-Fructopyranose
c.
Draw Cholesterol
d.
What's this and where is it found?
(2)
(2)
*
(2)
694:301 Second Exam, page 7
3.
Name ________________________
Give specific answers to each question:
a1.
Where is Acetylcholinesterase located?
a2.
Why is it such a fast-acting enzyme?
a3.
What happens to an animal exposed to an inhibitor of ACHase.
(2)
(2)
(2)
b1.
How do you identify Facilitated Diffusion? (What properties would you
look for?)
(2)
b2.
(2)
Is a Sodium Channel an example? How about the Calcium pump?
694:301 Second Exam, page 8
4.*
Name ________________________
a.
What cofactors are represented below?
b.
A ->
C ->
(4)
B
B
∆Go' = +5 kcal/mol
∆Go' = +7 kcal/mol
What is the standard free energy change for
A -> C? Show work and circle answer.
(4)
c.
Fill in the cofactors where needed:
(2)
COOH
CH2
CH2
COOH
-->
COOH
CH
||
--->
CH
COOH
COOH
HO --- C
CH2 --->
COOH
COOH
O=C
CH2
COOH
694:301 Second Exam, page 9
5.
Name ________________________
a.
How does epinephrine stimulate production of cyclic AMP in liver cells?
Diagram the process and explain briefly. What form of Protein Kinase is
activated by cyclic AMP?
(4)
b.
(4)
Give 4 examples of (different) processes mediated by 7TM receptors
______________________
_______________________
______________________
_______________________
c.
How do Calcium and other activators allow Protein Kinase C to become
active? A sketch or diagram would be very helpful in explaining this.
(2)