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Consulta: repository:"58"
Registros recuperados: 2.207
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The role of mycorrhization helper bacteria in the establishment and action of ectomycorrhizae
associations
Provedor de dados: 58
Autores: Rigamonte,Tatiana Alves; Pylro,Victor Satler; Duarte,Gabriela Frois.
More than 95 % short roots of most terrestrial plants are colonized by mycorrhizal fungi as soon as they emerge in the upper soil
profiles. The establishment of mycorrhizal association involves profound morphological and physiological changes in root and
fungus. It is affected by other rhizospheric microorganisms, specifically by the bacteria. Bacteria may have developed
mechanisms of selective interaction with surrounding microorganisms, with neutral or positive effects on mycorrhizal
associations, but negative effect on root pathogens in general. Because of the beneficial effect of bacteria on mycorrhizae, the
concept of Mycorrhization Helper Bacteria (MHB) was created. Five main actions of MHB on mycorrhizae were proposed: in the
receptivity of root to...
Tipo: Journal article
Palavras-chave: Mycorrhization Helper Bacteria; MHB; Ectomycorrhizal fungi; Pseudomonas.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400002
Initial pH of medium affects organic acids production but do not affect phosphate solubilization
Provedor de dados: 58
Autores: Marra,Leandro M.; Oliveira-Longatti,Silvia M. de; Soares,Cláudio R.F.S.; Lima,José M. de; Olivares,Fabio L.;
Moreira,Fatima M.S.
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform.
The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11
low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The
following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.),
UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains
UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH,
although without a significant difference...
Tipo: Journal article
Palavras-chave: Chelation; Propionic acid; Gluconic acid; Tartaric acid; Malic acid; Tropical soils.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200367
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli;
Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly
oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the
California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding
toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection
and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and
biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Journal article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535
Conjugative transfer of resistance determinants among human and bovine Streptococcus agalactiae
Provedor de dados: 58
Autores: Pinto,Tatiana Castro Abreu; Costa,Natália Silva; Corrêa,Ana Beatriz de Almeida; Oliveira,Ivi Cristina Menezes de;
Mattos,Marcos Correa de; Rosado,Alexandre Soares; Benchetrit,Leslie Claude.
Streptococcus agalactiae (GBS) is a major source of human perinatal diseases and bovine mastitis. Erythromycin (Ery) and
tetracycline (Tet) are usually employed for preventing human and bovine infections although resistance to such agents has become
common among GBS strains. Ery and Tet resistance genes are usually carried by conjugative transposons (CTns) belonging to the
Tn916 family, but their presence and transferability among GBS strains have not been totally explored. Here we evaluated the
presence of Tet resistance genes (tetM and tetO) and CTns among Ery-resistant (Ery-R) and Ery-susceptible (Ery-S) GBS strains
isolated from human and bovine sources; and analyzed the ability for transferring resistance determinants between strains from
both...
Tipo: Journal article
Palavras-chave: Streptococcus agalactiae (GBS); Conjugative transposons; Erythromycin resistance; Tetracycline resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300004
The potential inhibitory effect of cuminum cyminum, ziziphora clinopodioides and nigella sativa
essential oils on the growth of Aspergillus fumigatus and Aspergillus flavus
Provedor de dados: 58
Autores: Khosravi,A.R; Minooeianhaghighi,M.H; Shokri,H; Emami,S.A; Alavi,S.M; Asili,J.
The goals of this study were to evaluate the effectiveness of Cuminum cyminum, Ziziphora clinopodioides and Nigella sativa
essential oils to inhibit the growth of Aspergillus fumigatus and A. flavus and to evoke ultrastructural changes. The fungi were
cultured into RPMI 1640 media in the presence of oils at concentrations of 8, 6, 5, 4, 3, 2, 1.5, 1.25, 1, 0.75 and 0.5 mg/ml in broth
microdilution and 2, 1.5, 1 and 0.5 mg/ml in broth macrodilution methods with shaking for 48 h at 28ºC. Conidial and mycelial
samples exposed to 0.25, 0.5, 1, 1.5 and 2 mg essential oils/ml for 5 days in 2% yeast extract granulated plus 15% Saccharose
media were processed for transmission electron microscopy (TEM). Based on broth dilution methods, C. cyminum and to a
lesser...
Tipo: Journal article
Palavras-chave: Antifungal activity; Essential oil; Cuminum cyminum; Ziziphora clinopodioides; Nigella sativa; Aspergillus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100027
Combined application of origanum vulgare l. essential oil and acetic acid for controlling the growth of
staphylococcus aureus in foods
Provedor de dados: 58
Autores: Souza,Evandro Leite de; Barros,Jefferson Carneiro de; Conceição,Maria Lúcia da; Gomes Neto,Nelson Justino;
Costa,Ana Caroliny Vieira da.
This study evaluated the occurrence of an enhancing inhibitory effect of the combined application of Origanum vulgare L.
essential oil and acetic acid against Staphylococcus aureus by the determination of Fractional Inhibitory Concentration (FIC)
index and kill-time assay in nutrient broth, meat broth and in a food model (meat pieces). Acetic acid showed MIC and MFC of
0.6 and 1.25 µL.mL-1, respectively. For O. vulgare essential oil MIC and MBC were 1.25 and 2.5 µL.mL-1, respectively. FIC
indexes of the mixture of essential oil and acetic acid at MIC x ½ were £ 1.0, showing an additive effect. No synergy was found at
kill-time study. Anti-staphylococcal effect of the antimicrobials alone or in mixture (MIC x ½) was lower in meat than in nutrient
and meat...
Tipo: Journal article
Palavras-chave: Essential oil; Organic acid; Combined use; Anti-staphylococcal effect.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200032
Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants
and detergents on the enzyme activity
Provedor de dados: 58
Autores: Prazeres,Janaina Nicanuzia dos; Cruz,Juliana Aparecida Bortollotti; Pastore,Gláucia Maria.
Nowadays, there is a tendency of use of low temperature in laundry cleaning for both environmental and economical reasons,
which makes the use of enzymes in detergent products indispensable. Since lipases are efficient catalyst both in solution and at the
water-liquid interface, they are potentially suitable for lipid stain removal applications in industrial laundry and household
detergents. The effect of different commercial detergents and surfactants on enzymatic activity of lipase from Fusarium
oxysporum was observed through p-nitrophenylpalmitate (pNPP) assay. The enzyme was compatible with various ionic and
non-ionic surfactants as well as commercial detergents. Lipase activity was strongly inhibited by Sodium Dodecyl Sulphate
(SDS), but not by Triton...
Tipo: Journal article
Palavras-chave: Alkaline lipase; Fusarium oxysporum; Surfactants; Detergents.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400019
Protein profile analysis by SDS-PAGE of Mycoplasma Gallisepticum strains S6(208) and F-K810
grown in Hayflick's and Frey's media
Provedor de dados: 58
Autores: Ferraz,Priscila N.; Menezes,Claudio C. P.; Danelli,Maria das Graças M.; Lizeu,Jackeline de O. P.; Nascimento,Elmiro
R. do; Lucchesi,Daniela M.
The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media
were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media
composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer
traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is
specific for MG F-K810 strain, used as vaccine.
Tipo: Journal article
Palavras-chave: Mycoplasma gallisepticum; SDS-PAGE; Protein profile.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200010
Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by
Thermophilic Actinomycetes
Provedor de dados: 58
Autores: Singh,Renu; Kapoor,Vishal; Kumar,Vijay.
Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly
available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using
agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom
compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of
amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization
and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v) of various agro-industrial waste in production
medium containing...
Tipo: Journal article
Palavras-chave: Agro-industrial wastes; Thermophilic actinomycetes; Streptomyces; Amylase; Xylanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400039
Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria
Provedor de dados: 58
Autores: Nascimento,Gislene G. F.; Locatelli,Juliana; Freitas,Paulo C.; Silva,Giuliana L.
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant
microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the
following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm),
Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia
officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic
acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus
aromaticus...
Tipo: Journal article
Palavras-chave: Plant extracts activity; Medicinal plants; Antimicrobial activity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400003
Cultural conditions on the production of extracellular enzymes by Trichoderma isolates from tobacco
rhizosphere
Provedor de dados: 58
Autores: Rao,K.L.N Mallikharjuna; Raju,K. Siva; Ravisankar,H.
Abstract Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce
chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced
maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and
β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under different cultural conditions. The enzyme activities were
significantly influenced by acidic pH and the optimum temperature was 30 °C. The chitin and cell walls of Sclerotium rolfsii, as
carbon sources, supported the maximum and significantly higher chitinase activity by both isolates. The chitinase activity of
isolate ThJt1 was...
Tipo: Journal article
Palavras-chave: Trichoderma Cultural conditions Chitinase β -1 3-Glucanase Tobacco rhizosphere.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100025
Variation of Spirulina maxima biomass production in different depths of urea-used culture medium
Provedor de dados: 58
Autores: Affan,Md-Abu; Lee,Dae-Won; Al-Harbi,Salim Marzoog; Kim,Han-Jun; Abdulwassi,Najah Ibrahim; Heo,Soo-Jin;
Oh,Chulhong; Park,Heung-Sik; Ma,Chae Woo; Lee,Hyeon-Yong; Kang,Do-Hyung.
Fewer studies have assessed the outdoor cultivation of Spirulina maxima compared with S. platensis, although the protein content
of S. maxima is higher than S. platensis. Spirulina growth medium requires an increased amount of NaHCO3, Na2CO3, and
NaNO3, which increases the production cost. Therefore, the current study used a low-cost but high-efficiency biomass production
medium (Medium M-19) after testing 33 different media. The medium depth of 25 cm (group A) was sub-divided into A1 (50%
cover with a black curtain (PolyMax, 12 oz ultra-blackout), A2 (25% cover), and A3 (no cover). Similarly the medium depths of
30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively, and the effects of depth and
surface light...
Tipo: Journal article
Palavras-chave: Spirulina maxima; Fertilizer-grade urea; Medium depth; Surface cover; Outdoor mass culture.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400991
Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple
sterilization process
Provedor de dados: 58
Autores: Fesharaki,Parisa Jafari; Nazari,Pardis; Shakibaie,Mojtaba; Rezaie,Sassan; Banoee,Maryam; Abdollahi,Mohammad;
Shahverdi,Ahmad Reza.
The use of biologically derived metal nanoparticles for various proposes is going to be an issue of considerable importance; thus,
appropriate methods should be developed and tested for the biological synthesis and recovery of these nanoparticles from bacterial
cells. In this research study, a strain of Klebsiella pneumoniae was tested for its ability to synthesize elemental selenium
nanoparticles from selenium chloride. A broth of Klebsiella pneumoniae culture containing selenium nanoparticles was subjected
to sterilization at 121ºC and 17 psi for 20 minutes. Released selenium nanoparticles ranged in size from 100 to 550 nm, with an
average size of 245 nm. Our study also showed that no chemical changes occurred in selenium nanoparticles during the wet...
Tipo: Journal article
Palavras-chave: Selenium nanoparticles; Sterilization; Klebsiella pneumoniae; Synthesis; Recovery.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200028
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco
Valley
Provedor de dados: 58
Autores: Ponzzes-Gomes,Camila M.P.B.S. de; Mélo,Dângelly L.F.M. de; Santana,Caroline A.; Pereira,Giuliano E.;
Mendonça,Michelle O.C.; Gomes,Fátima C.O.; Oliveira,Evelyn S.; Barbosa Jr,Antonio M.; Trindade,Rita C.; Rosa,Carlos A.
The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape
varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley,
northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using
physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces
species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis,
Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina.
The population counts of these...
Tipo: Journal article
Palavras-chave: Brazilian wines; Saccharomyces cerevisiae; Indigenous strains; Non-Saccharomyces; Mitochondrial DNA
restriction analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200007
Prevalence of the paa gene (porcine attaching and effacing associated) in porcine enteropathogenic
Escherichia coli (PEPEC) associated with postweaning diarrhea in south Brazil
Provedor de dados: 58
Autores: Vidotto,Marilda C.; Florian,Elaine C.T.; Ono,Mario A.
Paa (porcine attaching and effacing associated) may be an important virulence factor E. coli of piglets with diarrhea. This study
showed for the first time in Brazil the prevalence of the paa gene (22%) in E. coli strains isolated from piglets and these isolates
also harboured genes for other adhesins and toxins LT II, STa and STb.
Tipo: Journal article
Palavras-chave: Escherichia coli; Pig; PEPEC; Paa gene; Postweaning diarrhea.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200030
Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas
aeruginosa
Provedor de dados: 58
Autores: Mitov,Ivan; Strateva,Tanya; Markova,Boyka.
The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from
cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups,
infection localization and antimicrobial resistance. The following frequencies in all studied strains were established: algD
(encoding GDP-mannose 6-dehydrogenase AlgD) - 91.1%, pilB (type IV fimbrial biogenesis protein PilB) - 23.8%, nan1
(neuraminidase) - 21.3%, lasB (elastase LasB) - 100%, plcH (haemolytic phospholipase C precursor) - 91.6%, exoS (exoenzyme
S) - 62.4%, and exoU (exoenzyme U) - 30.2%. The prevalence of nan1 was significantly higher (P<0.01) in CF isolates
(38.1%) than that in non-CF...
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Virulence genes; Polymerase chain reaction; Cystic fibrosis isolates; Non-cystic
fibrosis isolates.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300008
Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping
Provedor de dados: 58
Autores: Bezerra,Cristianne S.; Lima,José M.C.; Vilar,Janaina L.; Moreira,José L.B.; Frota,Cristiane C.
The hepatitis C virus (HCV) is classified into six different genotypes and their distribution is different throughout the world.
Epidemiologic studies are important to determine several characteristics of the virus, as well as the disease. This study analysed
the prevalence of HCV and its genotypes among patients from a leading hospital in Ceará, which is located in Northeast Brazil. A
total of 119 anti-HCV-seropositive patients, each having previously completed a questionnaire about risk behaviours related to
HCV infection were tested for HCV infection using a qualitative HCV polymerase chain reaction (PCR) assay and genotyping by
restriction fragment length polymorphism (RFLP). The detection was based on amplifying of the non-coding 5' region. Of the
119...
Tipo: Journal article
Palavras-chave: Hepatitis C virus; Genotypes; Epidemiology; Northeast Brazil; Viral hepatitis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400014
Horizontol dissemination of TEM- and SHV-typr beta-lactamase genes-carrying resistance plasmids
amongst clonical isolates of Enterobacteriaceae
Provedor de dados: 58
Autores: Ozgumus,Osman Birol; Tosun,Ilknur; Aydin,Faruk; Kilic,Ali Osman.
The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide.
Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. During a two-month
period in 2000, 133 clinical isolates of enterobacterial strains were randomly collected from outpatients and inpatients at a
university hospital in Turkey. The ESBL producing strains were determined by double-disk synergy (DDS) testing. Twenty ESBL
producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and
Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature
of the...
Tipo: Journal article
Palavras-chave: Horizontal spread; Resistance plasmids; ESBL; Transferência horizontal; ESBL; Plasmídios de resistência.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400007
Cellulase activities during decomposition of a submerged aquatic macrophyte (Utricularia
breviscapa): a microcosm assay
Provedor de dados: 58
Autores: Cunha-Santino,Marcela B. da; Bianchini Jr.,Irineu.
Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition.
Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the
following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources
added to Óleo lagoon (21º 36'S and 47º 49'W) water maintained in the dark under anaerobic condition at 15ºC, 20ºC, 25ºC and
30ºC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the
temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense
than...
Tipo: Journal article
Palavras-chave: Cellulase; Utricularia breviscapa; Cellulose.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200008
Cellulase and xylanase activity during the decomposition of three aquatic macrophytes in a tropical
oxbow lagoon
Provedor de dados: 58
Autores: Sciessere,L; Cunha-Santino,M. B; Bianchini Jr,I.
Due to the connection between enzymatic activity and degradation of different fractions of organic matter, enzyme assays can be
used to estimate degradation rates of particulate and dissolved organic carbon in freshwater systems. The aim of this study was to
quantify and model the enzymatic degradation involving the decomposition of macrophytes, describing temporal activity of
cellulases (EC 3.2.1.4 and EC 3.2.1.91) and xylanase (EC 3.2.1.8) during in situ decomposition of three aquatic macrophytes
(Salvinia sp., Eichhornia azurea and Cyperus giganteus) on the surface and water-sediment interface (w-s interface) of an oxbow
lagoon (Óleo lagoon) within a natural Brazilian Savanna Reserve. Overall, the enzymatic degradation of aquatic macrophytes in
Óleo...
Tipo: Journal article
Palavras-chave: Enzymes; Litterbags; Mass decay; Particulate organic matter.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300009
Dermatophytosis diagnosed at the Evandro Chagas Institute, Pará, Brazil
Provedor de dados: 58
Autores: Silveira-Gomes,Fabíola; Oliveira,Elaina Ferreira de; Nepomuceno,Lívia Barreto; Pimentel,Rosiane Ferreira;
Marques-da-Silva,Silvia Helena; Mesquita-da-Costa,Maurimélia.
Dermatophytosis is caused by a dermatophyte fungus that affects the stratum corneum and keratinized tissue. Dermatophyte
fungus has been reported worldwide as the causative agent of dermatophytosis, but the etio-epidemiological aspects of these
mycoses in the state of Pará remain unknown. The purpose of this study was to describe the etio-epidemiological profile of
dermatophytosis diagnosed in patients at the Evandro Chagas Institute from May 2005 to June 2006. A total of 494 patients were
admitted, and their samples were collected, submitted for direct microscopic examination using 20% KOH and cultured in
Sabouraud and Mycosel medium. The identification was based in macro and microscopic characteristics. Direct examinations
were positive in 13% (66/494)...
Tipo: Journal article
Palavras-chave: Dermatophytosis; Etio-epidemiology; Trichophyton; T. mentagrophytes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200016
Antimicrobial activity of some medicinal plants from the cerrado of the central-western region of
Brazil
Provedor de dados: 58
Autores: Violante,Ivana Maria Póvoa; Hamerski,Lidilhone; Garcez,Walmir Silva; Batista,Ana Lucia; Chang,Marilene Rodrigues;
Pott,Vali Joana; Garcez,Fernanda Rodrigues.
Ethanol extracts from six selected species from the Cerrado of the Central-Western region of Brazil, which are used in traditional
medicine for the treatment of infectious diseases and other medical conditions, namely Erythroxylum suberosum St. Hil.
(Erythroxylaceae), Hyptis crenata Pohl. ex Benth. (Lamiaceae), Roupala brasiliensis Klotz. (Proteaceae), Simarouba versicolor St.
Hil. (Simaroubaceae), Guazuma ulmifolia Lam. (Sterculiaceae) and Protium heptaphyllum (Aubl.) March. (Burseraceae), as well
as fractions resulting from partition of these crude extracts, were screened in vitro for their antifungal and antibacterial properties.
The antimicrobial activities were assessed by the broth microdilution assay against six control fungal strains, Candida...
Tipo: Journal article
Palavras-chave: Antifungal; Antibacterial; Antimicrobial; Artemia salina; Cerrado.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400009
Hazards in non-pasteurized milk on retail sale in Brazil: prevalence of Salmonella spp, Listeria
monocytogenes and chemical residues
Provedor de dados: 58
Autores: Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José
Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M.
Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling
raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk
producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the
microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides
(organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important
milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the
milking was manual. In 33% of...
Tipo: Journal article
Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007
Screening of microbial lipases and evalutaion of their potential to produce glycerides with high
gamma linolenic acid concentration
Provedor de dados: 58
Autores: Fregolente,Patricia B.L.; Fregolente,Leonardo V.; Maciel,Maria R.W.; Carvalho,Patricia O.
Gamma-linolenic acid (GLA, 18:3, cis- 6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family
biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains
with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids).
Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil,
strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state
fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a
special...
Tipo: Journal article
Palavras-chave: Gamma linolenic acid; Borage oil; Lipase; Geotrichum candidum.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400004
The relation between Escherichia coli surface functional groups' composition and their
physicochemical properties
Provedor de dados: 58
Autores: Hamadi,Fatima; Latrache,Hassan; Zahir,Hafida; Elghmari,Abderrahmene; Timinouni,Mohamed; Ellouali,Mostapha.
Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups'
composition were investigated. These characteristics were determined respectively by water contact angle measurements,
microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and
functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell
surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and
(OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.
Tipo: Journal article
Palavras-chave: Escherichia coli; Surface functional groups; Hydrophobicity; Electrophoretic mobility.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100003
Effects of pH and temperature on growth and glycerol production kinetics of two indigenous wine
strains of Saccharomyces cerevisiae from Turkey
Provedor de dados: 58
Autores: Yalcin,Seda Karasu; Ozbas,Z. Yesim.
The study was performed in a batch system in order to determine the effects of pH and temperature on growth and glycerol
production kinetics of two indigenous wine yeast strains Saccharomyces cerevisiae Kalecik 1 and Narince 3. The highest values of
dry mass and specific growth rate were obtained at pH 4.00 for both of the strains. Maximum specific glycerol production rates
were obtained at pH 5.92 and 6.27 for the strains Kalecik 1 and Narince 3, respectively. Kalecik 1 strain produced maximum 8.8
gL-1 of glycerol at pH 6.46. Maximum glycerol concentration obtained by the strain Narince 3 was 9.1 gL-1 at pH 6.48. Both
yeasts reached maximum specific growth rate at 30ºC. Optimum temperature range for glycerol production was determined as
25-30ºC for the...
Tipo: Journal article
Palavras-chave: Production kinetics; Glycerol; Growth parameters; Saccharomyces cerevisiae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200024
Phylogenetic relationships between Bacillus species and related genera inferred from 16s rDNA
sequences
Provedor de dados: 58
Autores: Wei Wang,Mi Sun.
Neigh-borjoining, maximum-parsimony, minimum-evolution, maximum-likelihood and Bayesian trees constructed based on 16S
rDNA sequences of 181 type strains of Bacillus species and related taxa manifested nine phylogenetic groups. The phylogenetic
analysis showed that Bacillus was not a monophyletic group. B. subtilis was in Group 1. Group 4, 6 and 8 respectively consisted
of thermophiles, halophilic or halotolerant bacilli and alkaliphilic bacilli. Group 2, 4 and 8 consisting of Bacillus species and
related genera demonstrated that the current taxonomic system did not agree well with the 16S rDNA evolutionary trees. The
position of Caryophanaceae and Planococcaceae in Group 2 suggested that they might be transferred into Bacillaceae, and the
heterogeneity of...
Tipo: Journal article
Palavras-chave: Bacillus phylogeny; Bayesian inference; Evolutionary trees; 16S rDNA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300013
Control of Listeria monocytogenes growth in soft cheeses by bacteriophage P100
Provedor de dados: 58
Autores: Silva,Elaine Nóbrega Gibson; Figueiredo,Ana Cláudia Leite; Miranda,Fernanda Araújo; Almeida,Rogeria Comastri de
Castro.
The purpose of this study was to determine the effect of bacteriophage P100 on strains of Listeria monocytogenes in artificially
inoculated soft cheeses. A mix of L. monocytogenes 1/2a and Scott A was inoculated in Minas Frescal and Coalho cheeses
(approximately 10(5) cfu/g) with the bacteriophage added thereafter (8.3 x 10(7) PFU/g). Samples were analyzed immediately,
and then stored at 10 ºC for seven days. At time zero, 30 min post-infection, the bacteriophage P100 reduced L. monocytogenes
counts by 2.3 log units in Minas Frescal cheese and by 2.1 log units in Coalho cheese, compared to controls without
bacteriophage. However, in samples stored under refrigeration for seven days, the bacteriophage P100 was only weakly
antilisterial, with the lowest...
Tipo: Journal article
Palavras-chave: Food safety; Listeria; Biocontrol; Bacteriophage; Cheese.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100003
Transcription of interferon stimulated genes in response to porcine rubulavirus infection in vitro
Provedor de dados: 58
Autores: Flores-Ocelotl,María del Rosario; Rosas-Murrieta,Nora Hilda; Vallejo-Ruiz,Verónica; Reyes-Leyva,Julio;
Herrera-Camacho,Irma; Santos-López,Gerardo.
Porcine rubulavirus (PoRV) is an emerging virus causing meningo-encephalitis and reproductive failures in pigs. Little is known
about the pathogenesis and immune evasion of this virus; therefore research on the mechanisms underlying tissue damage during
infection is essential. To explore these mechanisms, the effect of PoRV on the transcription of interferon (IFN) pathway members
was analyzed in vitro by semi-quantitative RT-PCR. Ten TCID50 of PoRV stimulated transcription of IFNα,
IFNβ, STAT1, STAT2, p48 and OAS genes in neuroblastoma cells, whereas infection with 100 TCID50 did not
stimulate transcription levels more than non-infected cells. When the cells were primed with IFNα, infection with 1
TCDI50 of PoRV sufficed to...
Tipo: Journal article
Palavras-chave: Porcine rubulavirus; Interferon; STAT; LPMV; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300041
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava
flour
Provedor de dados: 58
Autores: Coelho,Sheila Lorena de Araújo; Magalhães,Valter Cruz; Marbach,Phellippe Arthur Santos; Cazetta,Marcia Luciana.
Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars,
cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for
β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The
optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The
maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g
L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and
temperature 55 °C, and maintained thermal stability at 55 °C...
Tipo: Journal article
Palavras-chave: Microbial enzyme; Fermentation; Agro-industrial substrates.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120
Screening and optimization of pectin lyase and polygalacturonase activity from ginseng pathogen
Cylindrocarpon Destructans
Provedor de dados: 58
Autores: Sathiyaraj,Gayathri; Srinivasan,Sathiyaraj; Kim,Ho-Bin; Subramaniyam,Sathiyamoorthy; Lee,Ok Ran; Kim,Yeon-Ju;
Yang,Deok Chun.
Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. A Taguchi's orthogonal array
experimental design was applied to optimize the preliminary production of polygalacturonase (PG) and pectin lyase (PL) using
submerged culture condition. This method was applied to evaluate the significant parameters for the production of enzymes. The
process variables were pH, pectin concentration, incubation time and temperature. Optimization of process parameters resulted in
high levels of enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of 1.5% pectin.
Among different nitrogen sources, urea and peptone showed high production of PG and PL, respectively. The enzyme production
and...
Tipo: Journal article
Palavras-chave: C. destructans; Polygalacturonase; Pectin lyases; Ginseng.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200048
Inhibitory activity of compounds isolated from Polymnia sonchifolia on aflatoxin production by
Aspergillus flavus
Provedor de dados: 58
Autores: Pak,Adriana; Gonçalez,Edlayne; Felicio,Joana D'arc; Pinto,Marina Mori; Rossi,Maria Helena; Simoni,Isabela Cristina;
Lopes,Márcia Nasser.
Polymnia sonchifolia, commonly known as ";yacon";, was originally cultivated at Andes moutains in South America. Recently,
the specie attracted worldwide attention because of its wide range of uses, for example in the control of diabetes melitus, besides
the antifungal and pesticidal compounds were found in the leaves. This study describes the identification of two flavonoids: 3', 5,
7 trihydroxy-3, 4'-dimethoxyflavone (compound 1) and 3', 4', 5- trihydroxy-7-methoxy flavanone (compound 2) and two
sesquiterpenes lactones: enhydrin (compound 3) and a mixture of enhydrin and uvedalin (compound 4) isolated from Polymnia
sonchifolia leaves and their effects on the aflatoxin production by Aspergillus flavus. The identification of the compounds were
achieved by...
Tipo: Journal article
Palavras-chave: Polymnia sonchifolia; Flavonoids; Melampolides; Aflatoxin; Aspergillus flavus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200018
Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence
of L1 protein expression
Provedor de dados: 58
Autores: Góes,Luiz Gustavo Bentim; Freitas,Antonio Carlos de; Ferraz,Oilita Pereira; Rieger,Tania Tassinari; Santos,José
Ferreira dos; Pereira,Alexandre; Beçak,Willy; Lindsey,Charles J.; Stocco,Rita de Cassia.
The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the
Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe
genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.
Tipo: Journal article
Palavras-chave: Bovine Papillomavirus; BPV4; S2 expression cells.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100001
Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade,
Serbia
Provedor de dados: 58
Autores: Ristanovic,Elizabeta S.; Kitamura,Kayoko; Masuzawa,Toshiyuki; Milutinovic,Marija J.; Cekanac,Radovan M.;
Stajkovic,Novica T.; Zivanovic,Danijela M.
This is the first report of the molecular characterization and identification of Borrelia burgdorferi sensu lato strains isolated in
Serbia. Isolates A1, A2 and M1, from Ixodes ricinus, belong to Borrelia burgdorferi sensu stricto, while isolate K1 from
Apodemus flavicollis is a mixture of Borrelia afzelii and B. burgdorferi s.s.
Tipo: Journal article
Palavras-chave: Borrelia burgdorferi s.l.; Ixodes ricinus; Lyme borreliosis; Serbia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100004
Immobilization of lipase from Fusarium solani FS1
Provedor de dados: 58
Autores: Knight,Kirsty; Pimentel,Maria do Carmo Barros; Morais,Marcia Maria Camargo de; Ledingham,William M.; Lima
Filho,José Luiz de; Maia,Maria de Mascena Diniz.
Lipase from Fusarium solani FS1 was immobilized by covalent attachment to polyacrylamide beads and onto magnetized Dacron,
retaining 12% and 97% of activity, respectively. Lipase was also entrapped within polyacrylamide beads, retaining 53% of
activity. Investigations of the kinetic characteristics of the immobilized derivatives using triolein as substrate showed that lipase
immobilized onto polyacrilamide beads and Dacron did not follow Michaelis-Menten kinetics.
Tipo: Journal article
Palavras-chave: Fusarium solani FS1; Immobilized lipase; Enzyme kinetics; Dacron; Polyacrylamide beads.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300013
Isolation and identification of yeasts and filamentous fungi from yoghurts in Brazil
Provedor de dados: 58
Autores: Moreira,Silvia Regina; Schwan,Rosane Freitas; Carvalho,Eliana Pinheiro de; Wheals,Alan E.
Seventy-two cartons of yoghurt were sampled three times at monthly intervals from four different local manufacturers. Total
counts were close to 6 x 10(7) cells g-1 of yoghurt. Yeast counts varied from 1 to 2,700 g-1. There was no evidence of systematic
contamination at source but this longitudinal study revealed that ad hoc contamination and improper storage led to the higher
yeast counts. Contamination was generally higher in the hotter months but was lower overall than reported from other countries. A
total of 577 yeast isolates were identified belonging to ten species. The most abundant yeasts were, in order, Debaryomyces
hansenii, Saccharomyces cerevisiae, Mrakia frigida, Hansenula spp., Candida parapsilosis, Debaryomyces castellii and Candida...
Tipo: Journal article
Palavras-chave: Yeasts; Filamentous fungi; Yoghurts; Deterioration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200009
Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat
(Triticum aestivum)
Provedor de dados: 58
Autores: Orhan,Furkan.
ABSTRACT In the current study, 18 halotolerant and halophilic bacteria have been investigated for their plant growth promoting
abilities in vitro and in a hydroponic culture. The bacterial strains have been investigated for ammonia, indole-3-acetic acid and
1-aminocyclopropane-1-carboxylate-deaminase production, phosphate solubilisation and nitrogen fixation activities. Of the tested
bacteria, eight were inoculated with Triticum aestivum in a hydroponic culture. The investigated bacterial strains were found to
have different plant-growth promoting activities in vitro. Under salt stress (200 mM NaCl), the investigated bacterial strains
significantly increased the root and shoot length and total fresh weight of the plants. The growth rates of the plants...
Tipo: Journal article
Palavras-chave: Halophilic and halotolerant bacteria; Plant growth promoting; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300621
Antiviral activity of the Lippia graveolens (Mexican oregano) essential oil and its main compound
carvacrol against human and animal viruses
Provedor de dados: 58
Autores: Pilau,Marciele Ribas; Alves,Sydney Hartz; Weiblen,Rudi; Arenhart,Sandra; Cueto,Ana Paula; Lovato,Luciane
Teresinha.
Mexican oregano (Lippia graveolens) is a plant found in Mexico and Central America that is traditionally used as a medicinal
herb. In the present study, we investigated the antiviral activity of the essential oil of Mexican oregano and its major component,
carvacrol, against different human and animal viruses. The MTT test (3-4,5-dimethythiazol-2yl)-2,5-diphenyl tetrazolium
bromide) was conducted to determine the selectivity index (SI) of the essential oil, which was equal to 13.1, 7.4, 10.8, 9.7, and 7.2
for acyclovir-resistant herpes simplex virus type 1 (ACVR-HHV-1), acyclovir-sensitive HHV-1, human respiratory syncytial virus
(HRSV), bovine herpesvirus type 2 (BoHV-2), and bovine viral diarrhoea virus (BVDV), respectively. The human rotavirus (RV)
and...
Tipo: Journal article
Palavras-chave: Verbenaceae; Antimicrobial activity; RNA virus; DNA virus; Condimental herbs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400049
Antimicrobial susceptibility of Clostridium perfringens isolated from piglets with or without diarrhea
in Brazil
Provedor de dados: 58
Autores: Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Pires,Prhiscylla Sadanã; Cruz Júnior,Eduardo Coulaud da
Costa; Albefaro,Isabella Silva; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from
Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin
and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against
isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to
inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors
related to the antimicrobial resistance of C. perfringens.
Tipo: Journal article
Palavras-chave: Clostridium perfringens; Piglet; Diarrhea; Antibiotic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300027
Serological and molecular diversity of human rotavirus in São Paulo, Brazil
Provedor de dados: 58
Autores: Munford,Veridiana; Souza,Eloisa Correia; Caruzo,Thabata Alessandra Ramos; Martinez,Marina Baquerizo; Rácz,Maria
Lúcia.
From a total of 187 fecal samples from children with ages between 0 and 5 years, collected in the Hospital Universitário -USP,
Brazil, from 1994 to 1996, 54 (28.9%) were positive for rotavirus. Positive samples were characterized by electropherotyping,
subgrouping, G serotype and genotype and P genotype. Rotavirus electropherotypes were characterized in four different long
genome patterns (38.9%), one short genome pattern (34.0%) and 18.0% were characterized as an unusual pattern. Subgroup I was
found in 38.9% strains, subgroup II in 50.0% and 7.7% was subgroup nonI-nonII. For G serotypes, G2 was found in 59.3%, G1
was identified in 33.3% of strains, two samples showed mixtures of G1+G2 and one sample was G1+G3. Ten samples
characterized as serotype G2...
Tipo: Journal article
Palavras-chave: Rotavirus; Gastroenteritis; PCR; Serotypes; Genotypes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300014
Bioavailability of pollutants in bacterial communities of Rodrigo de Freitas Lagoon, Rio de Janeiro,
Brazil
Provedor de dados: 58
Autores: Fonseca,E.M. da; Baptista Neto,J.A.; McAlister,J.J.; Smith,B.J.; Crapez,M.A.C.
Processes involving heavy metals and other contaminants continue to present unsolved environmental questions. To advance the
understanding of geochemical processes that involve the bioavailability of contaminants, cores where collected in the Rodrigo de
Freitas lagoon, and analyzed for bacterial activity and metal concentrations. Results would suggest an extremely reducing
environment where organic substances seem to be the predominant agents responsible for this geochemical process. Analytical
data showed sulphate reduction to be the main agent driving this process, since this kind of bacteria was found to be active in all
of the samples analyzed. Esterase enzyme production did not signal the influence of heavy metals and hydrocarbon concentrations
and...
Tipo: Journal article
Palavras-chave: Heavy metals fraccionament; Bacterial communities; Sediment cores.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300027
A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of
tengchong, yunnan, China
Provedor de dados: 58
Autores: Ding,Jiannan; Zhang,Ruiyong; Yu,Yizun; Jin,Decai; Liang,Changli; Yi,Yang; Zhu,Wei; Xia,Jinlan.
A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring
sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm×1.0-1.31 µm in the medium containing
elemental sulfur and 1.5-2.22 µm×1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65)
and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including
various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor.
Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor.
Results of...
Tipo: Journal article
Palavras-chave: Isolation; Identification; Acidianus manzaensis; Bioleaching.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200016
Ribotyping and increasing trend of antibiotic resistance of Pseudomonas aeruginosa isolated in Iran
Provedor de dados: 58
Autores: Pourshafie,Mohammad Reza; Mousavi,Sayed Fazlolah; Parzadeh,Masoumeh.
The trend of antibiotic resistance, ribotyping and serotyping patterns of Pseudomonas aeruginosa collected since 1987 in Iran were
investigated. The results showed that among the aminoglycosides, amikacin was the most effective antibiotic against P.
aeruginosa with 98.4% susceptibility rate followed by tobramycin (73%) and gentamicin (71%). Of the cephalosporins,
susceptibility to ceftazidime was 93%. Among the antibiotics tested in vitro, ciproflaxacin was found to be the most effective
against the strains, with 98.4% susceptibility rate. The most predominant monovalent serotype was O:11 (34%). Other dominant
serotypes were O:5 (20%), O:1 (16%) and O:6 (15%). Thirteen percent of the isolates showed no agglutination with the tested
sera. A high percent of...
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Iran; Genotyping.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300010
Incorporation of nisin in natural casing for the control of spoilage microorganisms in vacuum
packaged sausage
Provedor de dados: 58
Autores: Barros,Joyce Regina de; Kunigk,Leo; Jurkiewicz,Cynthia Hyppolito.
This study aimed to evaluate the effectiveness of natural casing treatment with nisin and phosphoric acid on control of spoilage
microorganisms in vacuum packaged sausages. Ovine casings were dipped in the following baths: 1) 0.1% food grade phosphoric
acid; 2) 5.0 mg/L nisin; 3) 0.1% phosphoric acid and 5.0 mg/L nisin; and 4) sterile water (control). The sausages were produced in
a pilot plant, stuffed into the pretreated natural casings, vacuum packaged and stored at 4 and 10 °C for 56 days. The experiments
were performed according to a full factorial design 2³, totalizing 8 treatments that were repeated in 3 blocks. Aerobic plate counts
and lactic acid bacteria analysis were conducted at 1, 14, 28, 42 and 56 days of storage. Treatment of casings with...
Tipo: Journal article
Palavras-chave: Nisin; Bacteriocin; Sausage; Natural casing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400019
In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in
bioaugmented and biostimulated oil-contaminated soil
Provedor de dados: 58
Autores: Ángeles,Martínez-Toledo; Refugio,Rodríguez-Vázquez.
In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and
biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid
production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient
addition (nitrogen and phosphorus), P. putida addition, and addition of both (P. putida and nutrients). The results were compared
against a control treatment that consisted of adding only sterilized water to the soils. In treatment with native microorganisms
(non-irradiated soils) supplemented with P. putida, the removal of total petroleum hydrocarbons (TPH) was 40.6%, the
rhamnolipid production was...
Tipo: Journal article
Palavras-chave: Bioremediation; Irradiated soil; Total petroleum hydrocarbons; Rhamnolipids; P. putida.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200040
Antimicrobial effect of para-alkoxyphenylcarbamic acid esters containing substituted
N-phenylpiperazine moiety
Provedor de dados: 58
Autores: Malík,Ivan; Bukovský,Marián; Andriamainty,Fils; Gališ inová,Jana.
In
current
research,
nine
basic
esters
of
para-alkoxyphenylcarbamic
acid
with
incorporated
4-(4fluoro-/3-trifluoromethylphenyl)piperazin-1-yl fragment, 6i-6m and 8f-8i, were screened for their in vitro antimicrobial
activity against Candida albicans, Staphylococcus aureus and Escherichia coli, respectively. Taking into account the minimum
inhibitory concentration assay (MIC), as the most active against given yeast was evaluated 8i (MIC = 0.20 mg/mL), the most
lipophilic structure containing para-butoxy and trifluoromethyl substituents. Investigating the efficiency of the compounds bearing
only a single atom of fluorine and appropriate para-alkoxy side chain against Candida albicans, the cut-off effect was observed.
From evaluated homological series, the...
Tipo: Journal article
Palavras-chave: Phenylcarbamates; Substituted N-phenylpiperazines; Candida albicans.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200018
Applicability of the use of waste from different banana cultivars for the cultivation of the oyster
mushroom
Provedor de dados: 58
Autores: Carvalho,Cristiane Suely Melo de; Aguiar,Lorena Vieira Bentolila de; Sales-Campos,Ceci; Minhoni,Marli Teixeira de
Almeida; Andrade,Meire Cristina Nogueira de.
The objective of this research was to evaluate the oyster mushroom Pleurotus ostreatus- (Jacq.: Fr.) Kumm. cultivation in
substrates based on different combinations of wastes (leaf, pseudo-stem and pseudo-stem + leaf) and banana cultivars - Musa spp.
(Thap Maeo, Prata Anã, Pelipita and Caipira) during 49 days. Organic matter loss in the substrate by action of the fungus was also
evaluated during that period. It was verified that the pseudo-stem waste provided the best averages of biological efficiency among
all cultivars tested and best rates were obtained by Thap Maeo (61.5%). The highest organic matter loss (OML) was obtained from
pseudo-stem + leaf wastes (Prata Anã - 78.6%; Thap Maeo - 67.6%; Pelipita - 64.8%; Caipira - 60.6%). Therefore, the use of...
Tipo: Journal article
Palavras-chave: Pleurotus ostreatus; Mycelium; Organic matter; Biological efficiency; Supplementation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200048
Glass bead transformation method for gram-positive bacteria
Provedor de dados: 58
Autores: Rattanachaikunsopon,Pongsak; Phumkhachorn,Parichat.
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on
agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method,
introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
Tipo: Journal article
Palavras-chave: Transformation; Gram-positive bacteria; Glass bead.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400025
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride
Provedor de dados: 58
Autores: Matroudi,S.; Zamani,M.R.; Motallebi,M.
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of
Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have
been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene
contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid
sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33
proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli.
Tipo: Journal article
Palavras-chave: Trichoderma atroviride; Chit33; Chitinase activity; Gene structure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300005
Human respiratory syncytial virus detection in children admitted at a community hospital in
Botucatu, SP, Brazil
Provedor de dados: 58
Autores: Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz;
Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais.
Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral
infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age
presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence
and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80%
(12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied
period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between
May and July.
Tipo: Journal article
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015
Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of
diarrhea in dairy herds in Goiás, Brazil
Provedor de dados: 58
Autores: Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra
Ramos; Rácz,Maria-Lucia.
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás,
Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping,
PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I
rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four
samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one
sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were
detected and both were...
Tipo: Journal article
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015
Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular
typing methods during spontaneous fermentation for production of the artisanal cachaça
Provedor de dados: 58
Autores: Araújo,Roberta A.C.; Gomes,Fátima C.O.; Moreira,Elizabeth S.A.; Cisalpino,Patrícia S.; Rosa,Carlos A.
An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a
cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end
of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains
isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron
splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I
to XI) and one (XII) to a commercial strain of the bakery yeast. Pattern II (53.6% of the population) and pattern IV strains were...
Tipo: Journal article
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Fermentation; Molecular diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200006
Inhibitory effect of essential oils against Lactobacillus rhamnosus and starter culture in fermented
milk during its shelf-life period
Provedor de dados: 58
Autores: Moritz,Cristiane Mengue Feniman; Rall,Vera Lúcia Mores; Saeki,Margarida Júri; Fernandes Júnior,Ary.
The use of essential oils in foods has attracted great interest, due to their antagonistic action against pathogenic microorganisms.
However, this action is undesirable for probiotic foods, as products containing Lactobacillus rhamnosus. The aim of the present
study was to measure the sensitivity profile of L. rhamnosus and a yogurt starter culture in fermented milk, upon addition of
increasing concentrations of cinnamon, clove and mint essential oils. Essential oils were prepared by steam distillation, and
chemically characterised by gas chromatography-mass spectrometry (GC-MS) and determination of density. Survival curves were
obtained from counts of L. rhamnosus and the starter culture (alone and in combination), upon addition of 0.04% essential oils....
Tipo: Journal article
Palavras-chave: Lactobacillus rhamnosus; Starter culture; Essential oils; Fermented milk.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300042
The occurrence of Aeromonas in drinking water, tap water and the Porsuk River
Provedor de dados: 58
Autores: Kivanc,Merih; Yilmaz,Meral; Demir,Filiz.
The occurrence of Aeromonas spp. in the Porsuk River, public drinking water and tap water in the City of Eskisehir (Turkey) was
monitored. Fresh water samples were collected from several sampling sites during a period of one year. Total 102 typical colonies
of Aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by
biochemical tests. Further identifications of isolates were carried out first with the VITEK system (BioMe˜ rieux) and then
selected isolates from different phenotypes (VITEK types) were identified using the DuPont Qualicon RiboPrinter® system.
Aeromonas spp. was detected only in the samples from the Porsuk River. According to the results obtained with the VITEK
system, our isolates were...
Tipo: Journal article
Palavras-chave: Aeromonas spp; Drinking water; River water; Tap water; Riboprinter.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100016
Imipenem-resistant Acinetobacter baumannii carrying the ISAba1-bla OXA-23, 51 and ISAba1-bla
ADC-7 genes in Monteria, Colombia
Provedor de dados: 58
Autores: Martínez,Pedro; Mattar,Salim.
The purpose of this study was to identify the genes coding for resistance to ceftazidime and imipenem and describe the molecular
epidemiology of A. baumannii strains isolated from a clinical center in Colombia. Twenty isolates of imipenem-resistant A.
baumannii from an equal number of patients with nosocomial infections were obtained. Primers were used to amplify genes bla
IMP, bla VIM, bla OXA-23, bla OXA-24, bla OXA-58, bla OXA-51 and bla ADC-7. To detect insertion sequences ISAba1/bla
OXA-23, ISAba1/bla OXA-51 and ISAba1/bla ADC-7, mapping by PCR using combinations of reverse primers ISAba1 and
reverse primers of bla OXA-23, bla OXA-51 and bla ADC-7 were used. The amplification products were purified and cloned into
PCR 2.1-TOPO vector and transformed...
Tipo: Journal article
Palavras-chave: Nosocomial pathogens; Antimicrobial resistance; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400006
Double mutation of Saccharomyces cerevisiae for enhanced β-D-fructofuranosidase
fructohydrolase productivity and application of growth kinetics for parametric significance analysis
Provedor de dados: 58
Autores: Ali,Sikander; Aslam,Aafia; Hayyat,Muhammad Umar.
Abstract The kinetics of an extracellular β-D-fructofuranosidase fructohydrolase production by Saccharomyces
cerevisiae in a chemically defined medium, i.e., sucrose peptone agar yeast extract at pH 6, was investigated. The wild-type was
treated with a chemical mutagen, methyl methane sulfonate. Among the six mutants isolated, methyl methane sulfonate-V was
found to be a better enzyme producing strain (52 ± 2.4a U/mL). The maximum production (74 ± 3.1a U/mL) was accomplished
after at 48 h (68 ± 2.7a mg/mL protein). The mutants were stabilized at low levels of 5-fluoro-cytocine and the viable ones were
further processed for optimization of cultural conditions and nutritional requirements. The sucrose concentration, incubation
period and pH were...
Tipo: Journal article
Palavras-chave: Saccharomyces cerevisiae β -d-Fructofuranosidase fructohydrolase Kinetics and thermodynamics
Entropy and enthalpy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100136
Impact of environmental stress on biochemical parameters of bacteria reducing chromium
Provedor de dados: 58
Autores: Batool,Rida; Yrjälä,Kim; Hasnain,Shahida.
Chromium pollution is produced in connection with industrial processes like in tanneries. It has been suggested that
bioremediation could be a good option for clean up. The stress effect of variable chromate levels, pHs and growth temperatures on
biochemical parameters of two Cr(VI) reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium
Rb-2 was investigated. Transmission electrone microscopy (TEM) was performed to study the intracellular distribution of Cr(VI).
It was observed that initial stress of 1000 µgmL-1 caused significant enhancement of all studied biochemical parameters at pH 7.0
and growth temperature of 37 °C showing great bioremediation potential of the strains. Transmission electron microscopy
revealed that the...
Tipo: Journal article
Palavras-chave: Cr(VI); PH; Temperature; Biochemical parameters; TEM; FTIR spectroscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200028
NaCl stress impact on the key enzymes in glycolysis from Lactobacillus bulgaricus during
freeze-drying
Provedor de dados: 58
Autores: Li,Chun; Sun,Jinwei; Qi,Xiaoxi; Liu,Libo.
Abstract The viability of Lactobacillus bulgaricus in freeze-drying is of significant commercial interest to dairy industries. In the
study, L.bulgaricus demonstrated a significantly improved (p < 0.05) survival rate during freeze-drying when subjected to a
pre-stressed period under the conditions of 2% (w/v) NaCl for 2 h in the late growth phase. The main energy source for the life
activity of lactic acid bacteria is related to the glycolytic pathway. To investigate the phenomenon of this stress-related viability
improvement in L. bulgaricus, the activities and corresponding genes of key enzymes in glycolysis during 2% NaCl stress were
studied. NaCl stress significantly enhanced (p < 0.05) glucose utilization. The activities of glycolytic enzymes...
Tipo: Journal article
Palavras-chave: Lactobacillus bulgaricus; Freeze-drying; NaCl stress; Semi-quantitative RT-PCR; Glycolysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401193
Aggregatibacter actinomycetemcomitansarcB influences hydrophobic properties, biofilm formation
and adhesion to hydroxyapatite
Provedor de dados: 58
Autores: Longo,PL; Ota-Tsuzuki,C; Nunes,ACR; Fernandes,BL; Mintz,K; Fives-Taylor,P; Mayer,MPA.
The regulation of gene expression in the oral pathogen Aggregatibacter actinomycetemcomitans is still not fully elucidated.
ArcAB is a two-component system which allows facultative anaerobic bacteria to sense various respiratory growth conditions and
adapt their gene expression accordingly. This study investigated in A. actinomycetemcomitans the role of arcB on the regulation
of biofilm formation, adhesion to saliva coated hydroxyapatite (SHA) and the hydrophobic properties of the cell. These
phenotypic traits were determined for an A. actinomycetemcomitansarcB deficient type and a wild type strain. Differences in
hydrophobic properties were shown at early and late exponential growth phases under microaerobic incubation and at late
exponential phase under...
Tipo: Journal article
Palavras-chave: Two component system; Gene regulation; Adherence; Colonization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300018
Kinetic analysis of batch ethanol acetylation in isothermal non-stationary multiphase systems by
lyophilized mycelium of Aspergillus oryzae
Provedor de dados: 58
Autores: Palazzi,Emilio; Molinari,Francesco; Fabiano,Bruno; Pessoa Jr,Adalberto; Converti,Attilio.
A relatively complex network of reactions has been investigated, using as a network model the isothermal batch esterification of
acetic acid with ethanol in n-heptane catalyzed by lyophilized mycelium of Aspergillus oryzae. The kinetic analysis was firstly
carried out on the whole system, without any simplification, by means of the well-known integral method. Owing to the poor
results obtained by this way, we developed an alternative approach, combining initial rates and integral analysis and reducing the
number of empirical parameters to be determined by the use of equilibrium data. All the values of the parameters calculated
according to this "composite" approach to kinetic analysis well correlate with experimental data.
Tipo: Journal article
Palavras-chave: Aspergillus oryzae; Complex network; Enzyme reactions; Esterification; Organic solvent.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100048
Characterization of rhizobia that nodulate Arachis pintoi by RAPD analysis
Provedor de dados: 58
Autores: Pinto,Patrícia Pereira; Paiva,Edilson; Purcino,Hortência; Passos,Raul Vinícius Magalhães; Sá,Nadja Maria Horta.
The genetic relationships of 85 Arachis pintoi nodulating Rhizobium strains were determined using the random amplified
polymorphic DNA (RAPD) methods. The analysis included 75 strains isolated from Cerrado soils and 10 other ones of different
origins. The results indicated that there is a high level of similarity between these strains and that geographic distribution may
affect their phylogenetic relationship. In addition, the results allowed the selection of the most suitable primers for characterisation
of these Rhizobium strains which will be useful for implementation of competitiveness studies in Cerrado soils.
Tipo: Journal article
Palavras-chave: Rhizobium; Arachis pintoi; Cerrado soils.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200009
Epidemiological Survey of Listeria monocytogenes in a gravlax salmon processing line
Provedor de dados: 58
Autores: Cruz,C.D.; Silvestre,F.A.; Kinoshita,E.M.; Landgraf,M.; Franco,B.D.G.M.; Destro,M.T.
Listeria monocytogenes is a cause of concern to food industries, mainly for those producing ready-to-eat (RTE) products. This
microorganism can survive processing steps such as curing and cold smoking and is capable of growing under refrigeration
temperatures. Its presence in RTE fish products with extended shelf life may be a risk to the susceptible population. One example
of such a product is gravlax salmon; a refrigerated fish product not exposed to listericidal processes and was the subject of this
study. In order to evaluate the incidence and dissemination of L. monocytogenes 415 samples were collected at different steps of a
gravlax salmon processing line in São Paulo state, Brazil. L. monocytogenes was confirmed in salmon samples (41%), food
contact...
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Gravlax salmon; PFGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200032
Production and partial characterization of serine and metallo peptidases secreted by Aspergillus
fumigatus Fresenius in submerged and solid state fermentatio
Provedor de dados: 58
Autores: Silva,Ronivaldo Rodrigues da; Cabral,Tatiana Pereira de Freitas; Rodrigues,André; Hamilton,Cabral.
Enzyme production varies in different fermentation systems. Enzyme expression in different fermentation systems yields
important information for improving our understanding of enzymatic production induction. Comparative studies between
solid-state fermentation (SSF) using agro-industrial waste wheat bran and submerged fermentation (SmF) using synthetic media
were carried out to determinate the best parameters for peptidase production by the fungus Aspergillus fumigatus Fresen.
Variables tested include: the concentration of carbon and protein nitrogen sources, the size of the inoculum, the pH of the media,
temperature, and the length of the fermentation process. The best peptidase production during SSF was obtained after 96 hours
using wheat bran at 30 ºC...
Tipo: Journal article
Palavras-chave: Metallopeptidase; Serine peptidase; Fermentative parameters; Aspergillus fumigatus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100034
Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat
products
Provedor de dados: 58
Autores: Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z.
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic
acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and
fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as
Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly
identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic
bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them
presented...
Tipo: Journal article
Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008
The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs
Provedor de dados: 58
Autores: Coutinho,Selene Dall'Acqua; Burke,Julieta Catarina; Paula,Catia Dejuste de; Rodrigues,Miguel Trefaut;
Catão-Dias,José Luiz.
Many microorganisms are able to cause diseases in amphibians, and in the past few years one of the most reported has been
Batrachochytrium dendrobatidis. This fungus was first reported in Brazil in 2005; following this, other reports were made in
specimens deposited in museum collections, captive and free-living frogs. The aim of this study was to compare singleplex and
nested-PCR techniques to detect B. dendrobatidis in free-living and apparently healthy adult frogs from the Brazilian Atlantic
Forest. The sample collection area was a protected government park, with no general entrance permitted and no management of
the animals there. Swabs were taken from the skin of 107 animals without macroscopic lesions and they were maintained in
ethanol p.a. Fungal...
Tipo: Journal article
Palavras-chave: Chytridiomycosis; Batrachochytrium dendrobatidis; Frogs; Brazilian Atlantic Forest; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200551
Viral DNA sequences in peripheral blood and vertical transmission of the virus: a discussion about
BPV-1
Provedor de dados: 58
Autores: Freitas,Antonio Carlos de; Carvalho,Claudemir de; Brunner,Olga; Birgel-Junior,Eduardo Harry; Dellalibera,Alice Maria
Melville Paiva; Benesi,Fernando José; Gregory,Lilian; Beçak,Willy; Santos,Rita de Cassia Stocco dos.
This study has detected BPV-1 DNA sequences in wart, blood and plasma samples collected from animals affected by
papillomatosis, suggesting viral presence inside the cell. We sellected an animal in which we could detect BPV-1 DNA sequences
in wart, blood, placenta and amniotic liquid samples and her offspring which presented BPV-1 DNA sequences in blood sample
collected immediately after birth. These results show a possible vertical transmission of BPV-1.
Tipo: Journal article
Palavras-chave: BPV; Viral vertical transmission; PCR detection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500026
Performance of the Albicans ID2Ò chromogenic medium for rapid identification of Candida albicans
Provedor de dados: 58
Autores: Godoy-Martínez,Patricio; Azevedo,Ana C.; Reis,Viviane; Alves,Thelma; Almeida,Leila P.; Colombo,Arnaldo L.
The
aim
of
our
study
was
to
evaluate
the
accuracy
of
the
chromogenic
media
Albicans
ID2<FONT
FACE=Symbol>Ò</FONT> (bioMérieux, France) for the identification of Candida albicans among 330 yeast strains. All
C. albicans (100) and C. dubliniensis (20) strains exhibited blue color when cultured on Albicans ID2<FONT
FACE=Symbol>Ò</FONT>. However, the blue color was also exhibited by cultures of C. rugosa (30/30) and C.
tropicalis (3/50) isolates.
Tipo: Journal article
Palavras-chave: Candida albicans chromogenic medium Albicans ID2< FONT FACE=Symbol> Ò< /FONT> Media.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300004
Morphological and cytochemical characterization of spores and gills of Lepista sordida (Fungi:
Basidiomycota)
Provedor de dados: 58
Autores: Graf,Letícia V.; Schadeck,Ruth J. G.; Donatti,Lucélia; Buchi,Dorly F.
Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular
ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited
a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.
Tipo: Journal article
Palavras-chave: Basidiospores; Lipid body; Confocal microscopy; Electron microscopy; Lepista sordida.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300035
Penicillin production by wild isolates of Penicillium chrysogenum in Pakistan
Provedor de dados: 58
Autores: Rahman,Sajjad-Ur; Rasool,Muhammad Hidayat; Rafi,Muhammad.
The present study was aimed at exploring the native wild isolates of Penicillium chrysogenum series in terms of their penicillin
production potential. Apart from the standard medium, the efforts were made to utilize suitable agro-industrial wastes for the
maximum yield of penicillin. Two series of P. chrysogenum were isolated from local sources and named as P. chrysogenum series
UAF R1 and P. chrysogenum series UAF R2. The native series were found to possess better penicillin production potential than
the already reported series of P. chrysogenum. However, P. chrysogenum series UAF R1 was found to be the best candidate for
high yield of penicillin starting at 100 hour as compared to P. chrysogenum series UAF R2 which produced the highest yield of...
Tipo: Journal article
Palavras-chave: Penicillin; Penicillium chrysogenum; Corn Steep Liquor; Sugar Cane Bagasse.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200007
Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
Provedor de dados: 58
Autores: Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa.
Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism.
In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated
with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by
an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results
showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the
highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with
IgG for 24 hours. The...
Tipo: Journal article
Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
Characterization of outbreak hepatitis a isolates in five Tunisian childcare centers
Provedor de dados: 58
Autores: Gharbi-Khelifi,Hakima; Abid,Nabil Ben Salem; Sdiri,Khira; Harrath,Rafik; Beji,Abir; Bhiri,Leila; Billaudel,Sylviane;
Ferre,Virginie; Aouni,Mahjoub.
In the present study, epidemiological survey and molecular characterization of hepatitis A virus during an outbreak in five
Tunisian childcare centers in El-Mahres during October and November 2006 were carried out. Five well-water and five drinking
water samples were included in the present study. Serological investigation and molecular characterization were carried out. All
patients were IgM seropositive and the viral genome was detected in all clinical and well-water samples whereas it was not
detected in drinking water from the five childcare centers. Sequence analysis showed that all Tunisian strains belong to
sub-genotype IA. The genetic profile of the VP1/2A junction showed that the outbreak isolates underwent an amino acid
substitution which was...
Tipo: Journal article
Palavras-chave: Hepatitis A Virus; VP1 gene; VP1/2A junction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300046
Mutants with heteroresistance to amphotericin B and fluconazole in Candida
Provedor de dados: 58
Autores: Claudino,A.L.R.; Peixoto Junior,R.F.; Melhem,M.S.C.; Szeszs,M.W.; Lyon,J.P.; Chavasco,J.K.; Franco,M.C.
Several studies have reported the occurrence of infections caused by Candida yeasts as well as the increasing prevalence of non
albicans species. The aim of the present work is focused on the obtaining of heteroresistance to amphotericin B and fluconazole in
Candida species using two distinct methodologies: selection and induction. Resistant samples were obtained by selective pressure
using a medium with fluconazole for growth, followed by growth in a medium with amphotericin B. The selective pressure was
also created beginning with growth in amphotericin B medium followed by growth in fluconazole medium. Concomitantly,
samples were submitted to the induction of resistance through cultivation in increasing concentrations of fluconazole, followed
by...
Tipo: Journal article
Palavras-chave: Amphotericin B; Antifungal agents; Candida spp.; Fluconazole; Heteroresistance; Resistant mutants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400028
In vitro antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower
(Lythraceae)
Provedor de dados: 58
Autores: Parekh,Jigna; Chanda,Sumitra.
The antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower was evaluated at two different
concentrations by the agar well diffusion method. The methanol extract of the flower exhibited antibacterial activity at varied
levels except against Bacillus subtilis and Micrococcus flavus. The methanol extract was most active against Pseudomonas
pseudoalcaligenes. The extract was more active against Gram-negative bacteria as compared to Gram-positive. The inhibitory
effect of the extract was compared with standard antibiotics, amoxicillin and ciprofloxacin.
Tipo: Journal article
Palavras-chave: Woodfordia fruticosa; Antibacterial activity; Crude methanol extract.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200004
The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp.
rhamnosus
Provedor de dados: 58
Autores: Nancib,Aicha; Nancib,Nabil; Boubendir,Abdelhafid; Boudrant,Joseph.
The production of lactic acid from date juice by Lactobacillus caseisubsp. rhamnosus in batch and fed-batch cultures has been
investigated. The fed-batch culture system gave better results for lactic acid production and volumetric productivity. The aim of
this work is to determine the effects of the feeding rate and the concentration of the feeding medium containing date juice glucose
on the cell growth, the consumption of glucose and the lactic acid production by Lactobacillus casei subsp. rhamnosus in
fed-batch cultures. For this study, two concentrations of the feeding medium (62 and 100 g/L of date juice glucose) were tested at
different feeding rates (18, 22, 33, 75 and 150 mL/h). The highest volumetric productivity (1.3 g/L.h) and lactic acid yield...
Tipo: Journal article
Palavras-chave: Fed-batch culture; Lactic acid; Lactobacillus casei subsp. rhamnosus; Date juice.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300893
Clinical and Laboratory evaluation of measleslike rash in children and young adults
Provedor de dados: 58
Autores: Stewien,Klaus Eberhard; Lima,Lourdes Rehder de Andrade Vaz de; Botosso,Viviane Fongaro; Oliveira,Maria Isabel de;
Fagundes,Simone N.; Nogueira,Meri B.; Ragazzi,Selma Lopes Betta; Costa,Maria Tereza Zuluni da; Ejzenberg,Bernardo;
Durigon,Edison Luiz.
A clinical and laboratory evaluation of 11 children and young adults with measleslike rash was done during the measles outbreak
in the Greater São Paulo Metropolitan area at the end of 1996 and spread over the country during 1997. Measles was laboratory
confirmed in 07 patients by specific IgM detection in acute serum specimens using an IgM-capture EIA, by specific IgG
seroconversion in serum pairs, and by reverse transcription PCR and virus isolation in peripheral blood lymphocytes. Clinical
presentations were not always classic; one of the 07 cases had received measles vaccine and corresponded to modified clinical
case of measles. The 4 remaining cases were negative for measles and were diagnosed as exanthem subitum (2 cases), scarlet
fever and Kawasaki...
Tipo: Journal article
Palavras-chave: Measles; Clinical evaluation; Laboratory investigation; Polymerase chain reaction; Measles virus isolation;
Enzyme immunoassay.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400008
Antibacterial susceptibility patterns and cross-resistance of methicillin resistant and sensitive
Staphyloccus aureus isolated from the hospitalized patients in Shiraz, Iran
Provedor de dados: 58
Autores: Japoni,Aziz; Ziyaeyan,Mazyar; Jmalidoust,Marzieh; Farshad,Shohreh; Alborzi,Abdolvahab; Rafaatpour,Noraladdin;
Badiee,Parisa.
Nosocomial infections caused by methicillin-resistant staphylococci (MRSA) pose a serious problem in many countries. This
study aimed to determine the antibacterial susceptibility patterns of methicillin sensitive and resistant Staphylococcus aureus
isolates from the hospitalized patients. Totally 356 isolates of Staphylococcus aureus (S. aureus) including 200, 137 and 19
corresponding to MSSA, MRSA, and intermediate MRSA strains, respectively were isolated. Antibacterial susceptibility patterns
of the isolates to 14 antibiotics were examined using Kirby-Bauer method. MICs of 15 antibiotics to 156 MRSA isolates were
determined by E test method. Cross-resistances of MRSA isolates (137+19) to the other tested antibiotics were also determined.
S.aureus with...
Tipo: Journal article
Palavras-chave: MRSA; Minimum inhibitory concentration; Empirical therapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300005
Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
Provedor de dados: 58
Autores: González,Teresa; Licursi,María; Bonzo,Estela.
Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in
dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody
detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is
presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the
negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9%
which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and
Youden's...
Tipo: Journal article
Palavras-chave: Bovine leukemia; ELISA evaluation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100001
Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei
marketed in Natal, Brazil
Provedor de dados: 58
Autores: Melo,Ligia Maria Rodrigues de; Almeida,Dulce; Hofer,Ernesto; Reis,Cristhiane Moura Falavina dos; Theophilo,Grace
Nazareth Diogo; Santos,André Felipe das Mercês; Vieira,Regine Helena Silva dos Fernandes.
Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do
Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used
to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and
tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%)
presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed
intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or
resistance to...
Tipo: Journal article
Palavras-chave: Vibrio parahaemolyticus; Litopenaeus vannamei; Multiple antibiotic resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400032
Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae)
essential oil
Provedor de dados: 58
Autores: Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira.
Origanum vulgare L. (oregano), Lamiaceae, has been known as plant specie with prominent biological properties for a long time.
This study aimed to evaluate the antibacterial activity of Origanum vulgare essential oil on various Gram-positive and
Gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food
conservation micromodel. The results showed a strong antibacterial activity of the assayed essential oil noted by large growth
inhibition zones (30-37 mm). MIC values were between 20-40 µL/mL for the most bacteria strains. Essential oil was able to cause
significant (P<0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. In...
Tipo: Journal article
Palavras-chave: Origanum vulgare L.; Essential oil; Antimicrobial activity; Bacteria.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400023
Differences in the cell wall architecture of melanin lacking and melanin producing Cryptococcus
neoformans clinical isolates from India: an electron microscopic study
Provedor de dados: 58
Autores: Mandal,Piyali; Roy,Tara S.; Das,Taposh K.; Banerjee,Uma; Xess,Immaculata; Nosanchuk,Joshua D.
Cryptococcus neoformans is an important opportunistic fungal pathogen that causes life-threatening infection of the central
nervous system. A major virulence factor for C. neoformans is the production of melanin in the cell wall. Using transmission
electron microscopy, we studied the cell walls of three pairs of isolates obtained from patients with dual cryptococcal infections,
where a melanotic and an albino strain were isolated from the CSF of each patient. Transmission Electron Microscopy revealed
that the albino strains lacked a melanin layer whereas a melanin layer was associated with the cell wall of the melanotic strains,
comprising approximately 75% of the cell wall area. The cell wall size of the melanin producing cells was approximately double...
Tipo: Journal article
Palavras-chave: Cryptococcus neoformans; Melanin; TEM; Cell wall.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400015
Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap
plant
Provedor de dados: 58
Autores: Silva,F.V.; Simões-Araújo,J.L.; Silva Júnior,J.P.; Xavier,G.R.; Rumjanek,N.G.
The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated
in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their
phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal
primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also
sequenced with Y1, Y3 and six intermediate primers. The clustering analysis based on ARDRA profiles separated the Amazon
isolates in three subgroups, which formed a group apart from the reference isolates of Bradyrhizobium japonicum and
Bradyrhizobium elkanii. The clustering...
Tipo: Journal article
Palavras-chave: BNF; Bacteria; 16S rRNA; ARDRA; Sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200033
Fungal flora of the digestive tract of Rhodnius prolixus, Rhodnius neglectus, Diptelanogaster
maximus and Panstrongylus megistus, vectors of Trypanosoma cruzi, Chagas, 1909
Provedor de dados: 58
Autores: Moraes,Aurea Maria Lage de; Junqueira,Angela Cristina Vieira; Celano,Valéria; Costa,Gisela Lara da; Coura,José
Rodrigues.
This paper reports a study on the mycobiota in the digestive tract of four important species of triatomines: Rhodnius prolixus, R.
neglectus, Diptelanogaster maximus and Panstrongylus megistus. The digestive tracts of 90 adults and 425 nymphs of these four
triatomine species were studied and 365 fungal strains were isolated. The genera with the greatest number of species were
Aspergillus, Penicillium (14 species in each genus), Acremonium and Cladosporium (three species in each genus), and the most
frequent species, in decreasing order, were Aspergillus awamori, Penicillium corylophilum, Cladosporium herbarum and
Aspergillus niger. It was concluded that, among the isolated fungi, Aspergillus niger and Penicillium corylophilum might be part
of the natural...
Tipo: Journal article
Palavras-chave: Aspergillus spp; Fungi; Insect's digestive tract; Mycoflora; Penicillium spp; Triatomines.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300003
Identification of Bipolaris bicolor and Bipolaris sorokiniana on wheat seeds (Triticum aestivum L.) in
Brazil
Provedor de dados: 58
Autores: Morejon,Kendra R.; Moraes,Maria Heloiza D.; Bach,Erna E.
Diseases caused in wheat by Helminthosporium spp. have led to considerable yield and production losses. Different species in this
genus are associated with wheat seeds. In Brazil, spot blotch in wheat is caused by Bipolaris sorokiniana (Sacc) Schoem, and
another fungus Bipolaris bicolor (Mitra) Shoem that has been also isolated from wheat seeds. The current study was undertaken to
identify the most frequent fungus species that normaly infects wheat seeds and compared them with B. sorokiniana. The fungus
Bipolaris bicolor (Mitra) Shoem., isolated from wheat seeds cultivar IAPAR, was identified by taxonomic methods and compared
with the fungus Bipolaris sorokiniana (Sacc.) Shoem., in relation to growth characteristics on the seeds, as well as to growth...
Tipo: Journal article
Palavras-chave: Wheat; Bipolaris bicolor; Bipolaris sorokiniana.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300010
Activity of glycosidases from freshwater heterotrophic microorganisms on the degradation of
extracellular polysaccharide produced by Anabaena spiroides (Cyanobacteria)
Provedor de dados: 58
Autores: Colombo,Vanessa; Vieira,Armando A.H.; Moraes,Gilberto.
The activity of specific glycosidases during the degradation of the extracellular polysaccharide (EPS) produced by Anabaena
spiroides was determined using MUF-substrates (MUF-monosaccharides). Polysaccharide degradation was found to occur in a
two-phase process. The first consisted of high enzymatic activity that consumed 41% of the EPS at a relatively high rate, while the
second consumed the remaining polysaccharide (59%) at a slower rate. A transition phase from the higher to the slower
degradation rates was marked by a replacement of bacterial populations from coccoid to bacillus cells. During the degradation
process, the bacterial biomass increased with the decrease of EPS, as revealed by bacterial cell counts. The enzymatic activity
detected through...
Tipo: Journal article
Palavras-chave: Anabaena spiroides; Glycosidases; Extracellular polysaccharides; Degradation; Heterotrophic microorganisms.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100018
Microbiological monitoring of clean rooms in development of vaccines
Provedor de dados: 58
Autores: Utescher,Carla Lílian de Agostini; Franzolin,Marcia Regina; Trabulsi,Luiz Rachid; Gambale,Valderez.
The aim of the present work was to evaluate an environmental monitoring program for clean rooms, or classified environments,
involved in the filling and quality control of biological products produced by Butantan Institute, São Paulo, Brazil. This
monitoring established the quantification, characterization and seasonality of the microorganisms in air and operators and,
moreover, determined the alert and action limits. The total detectable microbial number showed some contrasts in installed air
purification systems and in the operational impact on adopted procedures. The typical microbial population consisted of
Staphylococcus sp, Micrococcus sp, Bacillus sp and Penicillium sp. The highest microorganism concentration occurred during
summer and springtime....
Tipo: Journal article
Palavras-chave: Environmental control; Viable particles; Clean rooms; Alert and action limits.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400023
Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract
Provedor de dados: 58
Autores: Jain,Sumat Chand; Jain,P.C.; Kango,Naveen.
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest
inulinase activity was observed with Dahlia extract (25.3 nkat mL-1) as carbon source. The enzyme activity was 1.4 folds higher
than that observed in media containing pure chicory inulin (17.8 nkat mL-1). The yeast showed good growth on a simple medium
containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and
120 rpm. Lowest inulinase yield (4.8 nkat mL-1) was seen in the medium containing glucose as C-source. Although varied
inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various
protein sources...
Tipo: Journal article
Palavras-chave: Inulinase; Dahlia; Fructose; Kluyveromyces marxianus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100007
Hyphal-like extension and pseudohyphal formation in industrial strains of yeasts induced by isoamyl
alcohol
Provedor de dados: 58
Autores: Ceccato-Antonini,Sandra Regina; Silva,Paula Cristina da.
Yeasts can produce pseudohyphae and hyphal-like extensions under certain growth conditions like isoamyl alcohol (IAA)
induction, a chief constituent of fusel oil, which is a subproduct from the ethanolic fermentation. The morphology switch from
yeast to a filamentous form can be troublesome to the process. In this work it was studied the influence of fusel alcohols, nitrogen
sources (ammonium sulphate and leucine) and glifosate (a chemical maturator for sugar cane) added to a complex medium on
some industrial strains of yeasts isolated from the fermentative process. Two industrial strains showed transition to hyphal-like
extensions or pseudohyphae (clusters of cells) upon addition of IAA from 0.3 to 0.9% /v. The alterations were reversible when the
yeasts...
Tipo: Journal article
Palavras-chave: Isoamyl alcohol; Ethanol; Yeast; Pseudohyphae; Hyphal-like extension.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300004
16S rRNA region based PCR protocol for identification and subtyping of Parvimonas micra
Provedor de dados: 58
Autores: Ota-Tsuzuki,C.; Brunheira,A.T.P.; Mayer,M.P.A.
The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by
PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be
clustered in five genotypes.
Tipo: Journal article
Palavras-chave: PCR-RFLP; 16S rRNA; Anaerobic cocci.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400001
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin
production and antimicrobial activity
Provedor de dados: 58
Autores: Carlos,Lanamar de Almeida; Amaral,Kenas Aguiar da Silva; Vieira,Ivo José Curcino; Mathias,Leda;
Braz-Filho,Raimundo; Samarão,Solange Silva; Vieira-da-Motta,Olney.
Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs
against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of
Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and
Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained
from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains
in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and
ELISA...
Tipo: Journal article
Palavras-chave: Rauvolfia grandiflora; Staphylococcus aureus; Enterotoxin; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011
Low genetic diversity among pathogenic strains of Erwinia psidii from Brazil
Provedor de dados: 58
Autores: Teixeira,Ana C. O.; Marques,Abi S. A.; Ferreira,Marisa A. S. V.
Erwinia psidii causes bacterial disease of guava in Brazil. Phenotypic and molecular characterization through rep-PCR
fingerprinting of 42 strains from different geographical regions showed that E. psidii populations in Brazil have a low level of
genetic diversity and suggest that contaminated plant material is the main source for pathogen dissemination in the country.
Tipo: Journal article
Palavras-chave: Guava bacterial blight; Psidium guajava; Rep-PCR fingerprinting.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300033
Brucella spp. isolation from dogs from commercial breeding kennels in São Paulo state, Brazil
Provedor de dados: 58
Autores: Keid,Lara B.; Soares,Rodrigo M.; Morais,Zenaide M.; Richtzenhain,Leonardo J.; Vasconcellos,Sílvio A.
Dogs from 12 commercial breeding kennels were submitted to clinical investigation and laboratorial tests for diagnosis of
Brucella spp. infection. The sampling was carried out between April 2000 and February 2002 and the laboratorial tests employed
were agar gel immunediffusion test (AGID) and blood culture. From 171 dogs examinated, 39 (22.8%) showed at least one
clinical sign compatible with brucellosis, 58 (33.91%) were AGID positive and 24 (14.03%) were positive by blood culture. Gram
negative bacterial cells with a biochemical pattern compatible with that of bacteria belonging to genus Brucella were isolated from
blood specimens of 24 animals. According to Kappa index and McNemar test, the association between AGID and blood culture
(k=0.360 with 95%...
Tipo: Journal article
Palavras-chave: Dogs; Brucellosis; Brucella canis; Diagnosis; Isolation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100027
Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB
P01-001: a potential probiotic
Provedor de dados: 58
Autores: Pancheniak,Elizete de F. R.; Maziero,Maike T.; Rodriguez-León,José A.; Parada,José L.; Spier,Michele R.;
Soccol,Carlos R.
Lactobacillus reuteri LPB P01-001 was isolated from the gastrointestinal tract of wild swine and was characterised by biochemical
testing and sequencing of gene 16S rRNA. A simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast
extract (1% p/v) was used in the production of this probiotic. The fermentative conditions were a) pH control at 6.5 and b) no pH
control; both were set at 37°C in a 12 L slightly stirred tank bioreactor. Fermentation parameters such as the specific growth rate,
productivity and yield of biomass, lactic and acetic acid levels were determined. L. reuteri LPB P01-001 behaves as an aciduric
bacteria because it grows better in a low pH medium without pH control. However, the lactic acid production yield was...
Tipo: Journal article
Palavras-chave: Probiotic; Lactobacillus reuteri; Molecular characterisation; Fermentation parameters; Lactic and acetic acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100015
PtSRR1, a putative Pisolithus tinctorius symbiosis related receptor gene is expressed during the first
hours of mycorrhizal interaction with Castanea sativa roots
Provedor de dados: 58
Autores: Acioli-Santos,B.; Malosso,E.; Calzavara-Silva,C. E.; Lima,C. E. P.; Figueiredo,A.; Sebastiana,M.; Pais,M. S.
PtSRR1 EST was previously identified in the first hours of Pisolithus tinctorius and Castanea sativa interaction. QRT-PCR
confirmed PtSRR1 early expression and in silico preliminary translated peptide analysis indicated a strong probability that
PtSRR1 be a transmembrane protein. These data stimulate the PtSRR1 gene research during ectomycorrhiza formation.
Tipo: Journal article
Palavras-chave: Ectomycorrhiza; Symbiosis related genes/proteins; Pisolithus tinctorius.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200015
Chemical and microbiological characterization of mangrove sediments after a large oil-spill in
Guanabara Bay - RJ - Brazil
Provedor de dados: 58
Autores: Maciel-Souza,Maria do Carmo; Macrae,Andrew; Volpon,Antonia Garcia Torres; Ferreira,Patrícia Silva;
Mendonça-Hagler,Leda Cristina.
Seventeen months after a 1,3 million L oil spill into Guanabara Bay, analyses of mangrove sediments showed that the three sites
closest to the spill remain highly polluted (>10 µg-g-1 polyaromatic hydrocarbons). A fourth site was less polluted,
from which most hydrocarbon degrading bacteria were isolated.
Tipo: Journal article
Palavras-chave: Mangrove; PAH; Bioprospecting; Bioremediation; Oil spill; Polyaromatic hydrocarbons.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300013
Enterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbiota of children
Provedor de dados: 58
Autores: Ignacio,Aline; Fernandes,Miriam Rodriguez; Avila-Campos,Mario Julio; Nakano,Viviane.
Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly
associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity
island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II
and III, based on the absence or presence of a BfPAI-flanking region, respectively. The incidence of ETBF and NTBF strains in
fecal samples isolated from children without acute diarrhea or any other intestinal disorders was determined. All 84 fecal samples
evaluated were B. fragilis-positive by PCR, four of them harbored the bft gene, 27 contained the NTBF pattern III DNA...
Tipo: Journal article
Palavras-chave: Bacteroides fragilis; Children; ETBF; NTBF.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401141
Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal
consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains
Provedor de dados: 58
Autores: Gouveia,E.M.M.F.; Silva,I.S.; Nakazato,G.; Onselem,V.J.V.; Corrêa,R.A.C.; Araujo,F.R.; Chang,M.R.
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on
enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then
MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and
monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as
occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E.
coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to
number of...
Tipo: Journal article
Palavras-chave: Enteropathogenic E. coli; In vivo experiment; Dogs; Phosphorylated 31 mannanoligo saccharides; PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200027
HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Provedor de dados: 58
Autores: Zepeda,Andrea B.; Figueroa,Carolina A.; Abdalla,Dulcineia S.P.; Maranhão,Andrea Q.; Ulloa,Patricio H.; Pessoa
Jr.,Adalberto; Farías,Jorge G.
Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared
to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate,
determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to establish the temperature
and methanol concentration conditions for optimal growth. The aim of this study is characterize the culture conditions through the
putative biomarkers in different conditions of temperature and methanol concentration. Three yeast cultures were performed: 3X =
3% methanol -10 °C, 4X = 3% methanol -30 °C, and 5X = 1% methanol -10 °C. The expression level of HIF-1α,
HSF-1,...
Tipo: Journal article
Palavras-chave: Recombinant yeast; Heat stress protein; Hypoxia; Chaperone.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200015
Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface
Provedor de dados: 58
Autores: Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper
wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its
enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the
residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant
changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all
insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in
biodeterioration...
Tipo: Journal article
Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface;
Polyester-imide; Enameled copper wires.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
Evaluation of the counterimmunoelectrophoresis reaction as a serodiagnosis test for swine
leptospirosis
Provedor de dados: 58
Autores: Genovez,Margareth Élide; Yasuda,Paulo Hideki; Vasconcellos,Silvio Arruda; Scarcelli,Eliana; Cardoso,Maristela
Vasconcellos; Girio,Raul da Silva.
The Counterimmunoelectrophoresis reaction (CIE) was tested as a genus specific test for swine leptospirosis diagnosis using three
soluble extracts obtained from Leptospira sp, serovars pomona, icterohaemorrhagiae and patoc. The extracts were treated with hot
Triton X-100 and applied to serum samples of animals divided in three groups: Group 1, 10 swines experimentally infected with
the Pomona strain; Group 2, 50 naturally infected swines and Group 3, 10 swines control. These animals were serologically
evaluated by CIE and by the Microscopic Agglutination Test (MAT), the WHO reference technique. Groups 1 and 3 were
monitored during a period of 93 days after infection (a.i.). Group 1 serum convertion took place around the 10th day a.i. by MAT
but ocurred...
Tipo: Journal article
Palavras-chave: Counterimmunoelectrophoresis; Swine leptospirosis; Seradiagnosis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200016
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent
Provedor de dados: 58
Autores: Hoshino-Shimizu,Sumie; Vaz-de-Lima,Lourdes Rehder de Andrade; Oliveira,Maria Isabel de; Pereira,Carlos Augusto;
Moura,Anísio de; Mendonça,Ronaldo Zucatelli.
Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the
IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits
to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in
comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a
positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with
unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL
presented...
Tipo: Journal article
Palavras-chave: Measles; IgM-measles reagent; Reagent quality control; Serodiagnosis; Seroepidemiology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016
Biocide tolerance in Salmonella from meats in Southern Spain
Provedor de dados: 58
Autores: Garrido,Antonio Marin; Burgos,Mª Jose Grande; Márquez,Mª Luisa Fernández; Aguayo,Mª Carmen López;
Pulido,Rubén Pérez; Árbol,Julia Toledo del; Gálvez,Antonio; López,Rosario Lucas.
Salmonella serovars sampled from meat products in Southern Spain (Andalucía) during the period 2002-2007 were analyzed in
this study. The serovars most frequently detected (in order) were Typhimurium, Enteritidis, Derby, Anatum and Rissen. Isolates (n
= 43) were tested for sensitivity to biocides, including the quaternary ammonium compounds benzalkonium chloride (BC),
cetrimide (CT) and hexadecylpyridinium chloride (HDP), and the bisphenols triclosan (TC) and hexachlorophene (CF). The
minimum inhibitory concentration (MIC) for the quaternary ammonium compounds was in the range of 25 to 50 mg/L for most
isolates, although a few isolates required much higher concentrations, up to 250 mg/L. Bisphenols showed higher inhibitory
activity, with a MIC of 2.5 to 25...
Tipo: Journal article
Palavras-chave: Salmonella; Biocides; Serovars; Meat; Chorizo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401177
Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1
Provedor de dados: 58
Autores: Tallur,Preeti N.; Mulla,Sikandar I.; Megadi,Veena B.; Talwar,Manjunatha P.; Ninnekar,Harichandra Z.
Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence.
Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have
investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as,
polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of
cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10
and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and
semi-continuous with shaken...
Tipo: Journal article
Palavras-chave: Degradation; Immobilization; Cypermethrin; Polyurethane foam; Micrococcus sp. strain CPN 1.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300667
Antimicrobial activity of Enterococcus faecium FAIR-E 198 against gram-positive pathogens
Provedor de dados: 58
Autores: Nascimento,Maristela da Silva do; Moreno,Izildinha; Kuaye,Arnaldo Yoshiteru.
This study investigated the antimicrobial activity of Enterococcus faecium FAIR-E 198 against Bacillus cereus, Listeria
monocytogenes and Staphylococcus aureus. Using the critical-dilution method, the bacteriocin produced by E. faecium FAIR-E
198 inhibited all L. monocytogenes strains evaluated (1,600 to 19,200 AU mL-1). However, none of the B. cereus and S. aureus
strains investigated were inhibited. The maximum activity of this bacteriocin (800 AU mL-1) was observed in MRS broth, while
the activity in milk was 100 AU mL-1. In the co-cultivation test in milk, B. cereus K1-B041 was reduced to below the detection
limit (1.00 log CFU mL-1) after 48 h. E. faecium reduced the initial L. monocytogenes Scott A population by 1 log CFU mL-1
after 3 h at 35ºC....
Tipo: Journal article
Palavras-chave: Enterococcus faecium; Bacteriocin; Biopreservation; Gram-positive pathogen.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100013
Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity
Provedor de dados: 58
Autores: Gong,Tianxiang; Li,Wanyi; Wang,Yueling; Jiang,Yan; Zhang,Qiang; Feng,Wei; Jiang,Zhonghua; Li,Mingyuan.
Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic
expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was
transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation
parameters: induce with 0.6 mM isopropylthiogalactoside (IPTG) at 34ºC in 2×YT medium and harvest at 6 h postinduction,
fusion protein TrxA-mBD2 was high expressed in the soluble fraction (>95%). After cleaved fusion protein by enterokinase,
soluble mature mBD2 was achieved 6 mg/L with a volumetric productivity. Purified recombinant mBD2 demonstrated clear
broad-spectrum antimicrobial...
Tipo: Journal article
Palavras-chave: Mouse beta defensin 2; Expression; Purification; Antimicrobial activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300043
Dermatophytes and other associated fungi in patients attending to some hospitals in Egypt
Provedor de dados: 58
Autores: Abd Elmegeed,Al Shimaa M.; Ouf,S.A.; Moussa,Tarek A.A.; Eltahlawi,S.M.R.
Dermatophytes are keratinophilic fungi that infect keratinized tissues causing diseases known as dermatophytoses. Dermatophytes
are classified in three genera, Epidermophyton, Microsporum, and Trichophyton. This investigation was performed to study the
prevalence of dermatomycosis among 640 patients being evaluated at the dermatology clinics at Kasr elainy, El-Husein and Said
Galal hospitals in Cairo and Giza between January 2005 and December 2006. The patients were checked for various diseases.
Tinea capitis was the most common clinical disease followed by tinea pedis and tinea corporis. Tinea cruris and tinea unguium
were the least in occurrence. Tinea versicolor also was detected. The most susceptible persons were children below 10 years
followed by...
Tipo: Journal article
Palavras-chave: Dermatophytosis; Dermatophytes; Fungi; Keratinophilic; Prevalence; Dermatology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300799
Biological and molecular characterization of Streptococcus crista strains isolated from human dental
biofilm by means of arbitrary primers - PCR (AP-PCR)
Provedor de dados: 58
Autores: Angelini,Michelle; Silveira,Wanderley Dias da; Campos,Tatiana Amabile de; Cai,Silvana; Castro,Antonio Fernando
Pestana de.
Streptococcus ssp are important components of the dental biofilm and Streptococcus crista is considered to be an interesting
model of bacterial interactions taking place in this biofilm. In the present work, S. crista strains were isolated from the dental
biofilm of Brazilian individuals and studied with respect to their biological characteristics and their molecular profile by means of
AP-PCR techniques, using the RR2, 434, OPR2, OPR8, and OPR13 primers. Results allowed us to build a similarity dendrogram.
Analysis of the similarity dendrogram allowed the separation of the studied strains into similarity groups. All isolates presented
fibril tufts by Transmission Electron Microscopy (TEM). These isolates were able to bind to salivary amylase and to adhere...
Tipo: Journal article
Palavras-chave: Biological characterization; Molecular characterization; Dendrogram analysis; Biofilm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200005
Thermal and mechanical shocks affecting the first flush of production of Lentinula edodes on
Eucalyptus saligna logs
Provedor de dados: 58
Autores: Shiomi,Humberto Franco; Minhoni,Marli Teixeira de Almeida; Machado,José Octávio; Cargnelutti Filho,Alberto.
The aim of this work was to evaluate the effect of thermal and mechanical shocks on the productivity of Lentinula edodes
colonized on 140 Eucalyptus saligna logs at different immersion times in water and in the first flush of production. The logs were
immersed in water at low temperature (16ºC) or environmental temperature (22ºC), for 6, 10, 14, 18, 22, 26 and 38 hours. The
mechanical shock treatment was accomplished by dropping the logs onto the floor three consecutive times, in a vertical position.
Water temperature and immersion time affected L. edodes yield, which increased two to four times when the logs were immersed
in cool water for the shortest times (6 and 10 hours). The mechanical shock treatment did not increase the sporophore yield.
Tipo: Journal article
Palavras-chave: Shiitake; Stress factors; Lentinula edodes; Sporophore yield.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200003
Antifungal activity of extracts from Piper aduncum leaves prepared by different solvents and
extraction techniques against dermatophytes Trichophyton rubrum and Trichophyton interdigitale
Provedor de dados: 58
Autores: Santos,Maximillan Leite; Magalhães,Chaiana Froés; Rosa,Marcelo Barcellos da; Santos,Daniel de Assis;
Brasileiro,Beatriz Gonçalves; Carvalho,Leandro Machado de; Silva,Marcelo Barreto da; Zani,Carlos Leomar; Siqueira,Ezequias
Pessoa de; Peres,Rodrigo Loreto; Andrade,Anderson Assunção.
The effects of different solvents and extraction techniques upon the phytochemical profile and anti-Trichophyton activity of
extracts from Piper aduncum leaves were evaluated. Extract done by maceration method with ethanol has higher content of
sesquiterpenes and antifungal activity. This extract may be useful as an alternative treatment for dermatophytosis.
Tipo: Journal article
Palavras-chave: Antifungal activity; Piper aduncum L.; Dermatophytes; Trichophyton; Extraction techniques.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400035
OCCURRENCY OF MALASSEZIA PACHYDERMATIS AND OTHER INFECTIOUS AGENTS AS
CAUSE OF EXTERNAL OTITIS IN DOGS FROM RIO GRANDE DO SUL STATE, BRAZIL
(1996/1997)
Provedor de dados: 58
Autores: Nobre,Márcia de Oliveira; Castro,Ângela Pötter de; Nascente,Patrícia da Silva; Ferreiro,Laerte; Meireles,Mario Carlos
A.
The purpose of this work was to evaluate the frequency of Malassezia pachydermatis infection and other infectious agents in dogs
with external otitis and with healthy auditory tubes. Clinical manifestations of external otitis were evaluated. Samples from the
auditory tube of 102 dogs with otitis and from 32 healthy dogs were submitted to direct microscopic examination and cultured in
blood agar and Sabouraud dextrose agar with chloramphenicol and cycloheximide. Direct examination showed more than ten cells
of M. pachydermatis in 52.0% of the samples from dogs with otitis, but in only 21.8% of the healthy auditory tube samples. M.
pachydermatis was isolated in 37.5% of the samples from dogs with healthy auditory tube and 76.5% (p<0.01) of the samples...
Tipo: Journal article
Palavras-chave: Malassezia pachydermatis; Staphylococcus aureus; Otitis; Dog.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300017
Partial characterization of antagonistic substance produced by a Clostridium butyricum strain
Provedor de dados: 58
Autores: Caires,Nely Cristina Medeiros; Matos,Aline Magalhães; Farias,Luiz de Macedo; Carvalho,Maria Auxiliadora Roque de;
Drummond,Regina Maria Nardi; Nicoli,Jacques Robert; Ribeiro Sobrinho,Antônio Paulino.
The production of antagonistic substance by bacterium present in the infected root canal system (RCS) probably is an important
ecological factor for its successful colonization of the local. The objective of this study was to partially characterize an
antagonistic substance produced by a Clostridium butyricum isolated from infected RCS.Production of inhibitory compound was
evaluated by the agar double layer diffusion technique using Fusobacterium nucleatum and Bifidobacterium adolescentis as
indicator bacteria. The physicochemical and biochemical factors tested for the partial characterization were influence of pH and
temperature and susceptibility to the action of some proteolytic enzymes. An inhibition zone was observed against the two
indicator strains...
Tipo: Journal article
Palavras-chave: Clostridium butyricum; Fusobacterium nucleatum; Bifidobacterium adolescentis; Antagonism; Root canal
system.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200014
Detection of thermophilic Campylobacter sp. in raw chicken sausages by methods ISO 10272: 2006 in
Curitiba - Parana State - Brazil
Provedor de dados: 58
Autores: Konell,K.; Gelinsk,M.A.; Benetti,T.M.; Abrahão,W.M.
The aim of this study was the detection of Campylobacter sp. in raw chicken sausages using the methods ISO 10272-1 and ISO
10272-2. The overall prevalence of Campylobacter sp. in the samples tested was 16.67%, representing a serious risk to
the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are
not adopted. Furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of
contaminated raw or undercooked poultry meat, which constitute the main vehicle of this infection.
Tipo: Journal article
Palavras-chave: Campylobacter sp.; Poultry meat; Chicken sausages.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400053
Evaluation of a fungal collection as certified reference material producer and as a biological resource
center
Provedor de dados: 58
Autores: Forti,Tatiana; Souto,Aline da S.S.; Nascimento,Carlos Roberto S. do; Nishikawa,Marilia M.; Hubner,Marise T.W.;
Sabagh,Fernanda P.; Temporal,Rosane Maria; Rodrigues,Janaína M.; Silva,Manuela da.
Abstract Considering the absence of standards for culture collections and more specifically for biological resource centers in the
world, in addition to the absence of certified biological material in Brazil, this study aimed to evaluate a Fungal Collection from
Fiocruz, as a producer of certified reference material and as Biological Resource Center (BRC). For this evaluation, a checklist
based on the requirements of ABNT ISO GUIA34:2012 correlated with the ABNT NBR ISO/IEC17025:2005, was designed and
applied. Complementing the implementation of the checklist, an internal audit was performed. An evaluation of this Collection as
a BRC was also conducted following the requirements of the NIT-DICLA-061, the Brazilian internal standard from Inmetro,
based on...
Tipo: Journal article
Palavras-chave: Fungal collection; Certified reference material; Biological Resource Center; ABNT NBR ISO/IEC 17025;
NIT-DICLA-061.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200403
Characterization of Aujeszky's disease virus isolates from South and Southeast Brazil by RFLP
analysis
Provedor de dados: 58
Autores: Piatti,Rosa Maria; Ikuno,Alice Akimi; Cunha,Elenice Sequetin; D'Ambros,Régia; Gregori,Fábio; Soares,Rodrigo
Martins; Cortez,Adriana; Richtzenhain,Leonardo José.
The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from
1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs,
1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as
genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of
brazilian ADV strains, we could demonstrate the occurence in Brazil of the genomic types I and II, with a large predominance of
genomic type II.
Tipo: Journal article
Palavras-chave: Aujeszky's disease virus; Swine; Genomic RFLP; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200015
Low doses of gamma radiation in the management of postharvest Lasiodiplodia theobromae in
mangos
Provedor de dados: 58
Autores: Santos,Alice Maria Gonçalves; Lins,Severina Rodrigues Oliveira; Silva,Josenilda Maria da; Oliveira,Sônia Maria Alves
de.
The postharvest life of mango is limited by the development of pathogens, especially fungi that cause rot, among which stands out
the Lasiodiplodia theobromae. Several control methods have been employed to minimize the damages caused by this fungus,
chemical control can leave residues to man and nature; physical control by the use of gamma radiation in combination with
modified atmosphere and cold storage. The use of gamma radiation helps to reduce the severity of the pathogen assist in the
ripening process of fruits, even at low doses (0.25, 0.35 and 0.45 kGy) chemical properties such as pH, soluble solids, acid
ascorbic, titratable acidity and also the quality parameters of the pulp showed no damage that are ideal for trade and consumption
of mangoes....
Tipo: Journal article
Palavras-chave: Mangifera indica; Ionizing radiation; Quality.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300841
Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger
and Aspergillus terreus under SSF of banana peels
Provedor de dados: 58
Autores: Rehman,Shazia; Aslam,Hina; Ahmad,Aqeel; Khan,Shakeel Ahmed; Sohail,Muhammad.
Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall
degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and
Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases
(β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is
the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal
strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme
preparations obtained from monoculture and...
Tipo: Journal article
Palavras-chave: Solid state fermentation; Plant cell wall degrading enzymes (CWDE); Banana peels; Aspergillus strains;
Enzymatic hydrolysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400045
Antibacterial activity of extracts of Acacia aroma against methicillin-resistant and
methicillin-sensitive Staphylococcus
Provedor de dados: 58
Autores: Mattana,C.M.; Satorres,S.E.; Sosa,A.; Fusco,M.; Alcaráz,L.E.
Antibacterial activity of organic and aqueous extracts of Acacia aroma was evaluated against methicillin-resistant Staphylococcus
aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus epidermidis.
Inhibition of bacterial growth was determined using agar diffusion and bioautographic methods. Among all assayed organic
extracts only ethanolic and ethyl acetate extracts presented highest activities against all tested Staphylococcus strains with
minimal inhibitory concentration (MIC) values ranging from 2.5 to 10 mg/ml and from 2.5 to 5 mg/ml respectively. The aqueous
extracts show little antibacterial activity against Staphylococcus strains. The bioautography assay demonstrated well-defined
growth inhibition...
Tipo: Journal article
Palavras-chave: Acacia aroma; Antibacterial activity; Staphylococcus; Tusca.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300007
Detection of integrons among multi-drug resistant (MDR) Escherichia coli strains isolated from
clinical specimens in northern west of Iran
Provedor de dados: 58
Autores: Rezaee,Mohammad Ahangarzadeh; Sheikhalizadeh,Vajihe; Hasani,Alka.
Transference of resistance determinants by integrons is one of the important factors that can contribute to the increase in
multi-resistant bacteria. We determined the prevalence and class of integrons among multi-drug resistant (MDR) Escherichia coli
strains isolated from clinical specimens in Tabriz teaching hospitals. Firstly, susceptibility of 140 isolates to 13 antibiotics was
determined using the disc diffusion method. Then, prevalence and class of integrons was detected in MDR strains by PCR-RFLP.
One hundred five (75%) of total 140 isolates were uropathogenic Escherichia coli (UPEC). Other pathotypes included were:
diarrheagenic Escherichia coli (13; 9.3%), sepsis-associated E. coli (5; 3.6%) and newborn meningitis-associated E. coli (2;
1.4%)....
Tipo: Journal article
Palavras-chave: Integron; Escherichia coli; Multi-drug resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400010
The function of lexical motifs in the organization of the Actinomycetes 5S rRNAs
Provedor de dados: 58
Autores: Rodrigues-Subacius,Sandra M; Padilla,Gabriel.
This work shows results obtained by employing the linguistic method to identify biologically meaningful sites in Actinomycetes
5S rRNAs. The approach adopted identifies triplet-words, along the base sequence of 5S rRNA, located mainly at the alpha and
beta domains of the 5S secondary structure. There are triplet-words representing universal protein binding sites that include
important prokaryote signatures, and sites strategically located in critical regions related to the formation of the 5S
ribonucleoproteins (RNP) complex. In those sites, where the GC pressure promoted substitutions, the analysis demonstrates that
alterations did not affect their biological significance. Sites formed by GGY (or more rarely GGR), continued to play an important
role as...
Tipo: Journal article
Palavras-chave: Actinomycetes; 5S rRNA; GC pressure; Linguistic analysis; Lexical motifs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300036
Lack of association between genotypes and virulence factors in C. albicans strains isolated from
vaginal secretion
Provedor de dados: 58
Autores: Shinobu,Cristiane Suemi; Ogatta,Sueli Fumie Yamada; Bizerra,Fenando; Furlaneto,Luciana; Peralta,Rosane Marina;
Svidzinski,Terezinha Inez Estivalet; Consolaro,Marcia Edilaine Lopes.
The physiopathogenesis of vulvovaginal candidiasis (VVC) is still not completely elucidated. The objective of this study was to
evaluate if there is a relationship between the different genotypes of Candida albicans, their main agent and the virulence of this
yeast in vaginal isolates, and to check if there are laboratorial markers that can predict the ability of each isolate to develop VVC
independently of symptoms. The production of exoenzymes protease, phospholipase and haemolysin, resistance to hydrogen
peroxide, and the genotype were determined. Genotype A was predominant (75%), protease, phospholipase and haemolytic
activity were highly expressed, and the majority of the yeasts were sensitive to H2O2 in 1 and 2 hours of exposure, suggesting
that...
Tipo: Journal article
Palavras-chave: C. albicans; Vulvovaginal candidiasis; Genotype; Virulence factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300015
Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its
properties
Provedor de dados: 58
Autores: Bonilha,Paulo Roberto Martins; Menocci,Vivian; Goulart,Antonio José; Polizeli,Maria de Lourdes Teixeira de Moraes;
Monti,Rubens.
Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular
transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis.
CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and
amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with
sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production
capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range.
After...
Tipo: Journal article
Palavras-chave: Cyclodextrin glycosyltransferase; CGTase production; Bacilluslicheniformis; Bacillus beta-CD.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022
Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates
Provedor de dados: 58
Autores: Mirzarazi,Mahsa; Rezatofighi,Seyedeh Elham; Pourmahdi,Mahdi; Mohajeri,Mohamad Reza.
To determine the presence of some toxins of diarrheagenic Escherichia coli (DEC) in uropathogenic E. coli (UPEC), 138 urinary
tract infection (UTI)-causing UPECs were analyzed. The astA, set, sen and cdtB genes were detected in 13 (9.4%), 2 (1.3%), 13
(9.4%) and 0 (0%) of UPEC isolates respectively. The results show that some genes encoding toxins can be transferred from DEC
pathotypes to UPECs therefore these isolates can transform into potential diarrhea-causing agents.
Tipo: Journal article
Palavras-chave: Uropathogenic Escherichia coli; Toxins; Phylogenetic groups; Set; Sen; AstA and cdt genes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100155
Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine
herpesviruses in cell culture
Provedor de dados: 58
Autores: Bruggemann,Rafaela; Matsuo Orlandi,Janaina; Benati,Fabricio Jose; Faccin,Ligia Carla; Mantovani,Mario Sergio;
Nozawa,Carlos; Linhares,Rosa Elisa Carvalho.
The aqueous extract of Agaricus blazei Murill ss. Heinem, a basidiomycete native from Brazil, frequently used by popular
medicine, mainly in the form of tea, was assessed to its antiviral action against herpes simplex type 1 (HSV-1) and bovine herpes
type 1 (BoHV-1) in HEp-2 cell culture. Viral replication inhibition was evaluated by plaque assay and immunofluorescence test.
The extract demonstrated virucide action for both viruses, being more effective against HSV-1, inhibiting its infectivity in 78.4
and 73.9% at the concentrations of 50 and 100 µg/mL, respectively moreover, reduction in 47% the number of fluorescent cells
was observed for both concentrations. The extract also showed discrete therapeutic activity. These results suggest that A. blazei...
Tipo: Journal article
Palavras-chave: Agaricus blazei; BoHV-1; HSV-1; Antiviral activity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400029
Identification of genes expressed by Cryptococcus gattii during iron deprivation
Provedor de dados: 58
Autores: Paula,Daphine Ariadne Jesus de; Silva,Lívia Kmetzsch Rosa e; Staats,Charley Christian; Vainstein,Marilene H.;
Joanoni,Ana Lúcia Pinto; Nakazato,Luciano; Dutra,Valéria.
Cryptococcus neoformans and C. gattii are pathogenic yeasts that cause life-threatening diseases in humans and animals. Iron is an
essential nutrient for virtually every organism as it functions as a cofactor in numerous essential enzymatic reactions. In the
literature, the competition for iron between microbes and mammalian hosts during infection is well documented. In this study, we
used representational difference analysis (RDA) in order to gain a better understanding of how C. gattii responds to iron
starvation. A total of 15 and 29 genes were identified as having altered expression levels due to iron depletion after 3 h and 12 h,
respectively. Of these, eight genes were identified in both libraries. The transcripts were related to many biological...
Tipo: Journal article
Palavras-chave: Cryptococcus gattii; Iron; Representational difference analysis; Yeast.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300008
Identification of Lactobacillus spp. from broiler litter in Brazil
Provedor de dados: 58
Autores: Paço,Ronaldo S.; Leme,Ivani Lucia; Bottino,José Américo; Ferreira,Antônio José Piantino.
Lactobacillus spp. were identified in 100 broiler litter samples collected from different poultry-rearing regions in Brazil. Ten
different Lactobacillus species were identified: L. plantarum, L.casei subsp. pesudoplantarum, L. delbrueckii subsp. delbrueckii,
L. reuteri, L. murinus, L. agilis, L. delbrueckii subsp. lactis, L. salivarus subsp. salicinus, L. viridenscens and L. amylophilus.
Tipo: Journal article
Palavras-chave: Lactobacillus spp.; Broiler litter; Chicken.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300010
Screening of endoglucanase-producing bacteria in the saline rhizosphere of Rhizophora mangle
Provedor de dados: 58
Autores: Sá,André Luís Braghini; Dias,Armando Cavalcante Franco; Quecine,Maria Carolina; Cotta,Simone Raposo;
Fasanella,Cristiane Cipola; Andreote,Fernando Dini; Melo,Itamar Soares de.
In screening the culturable endoglucanase-producing bacteria in the rhizosphere of Rhizophora mangle, we found a prevalence of
genera Bacillus and Paenibacillus. These bacteria revealed different activities in endoglucolysis and biofilm formation when
exposed to specific NaCl concentrations, indicating modulated growth under natural variations in mangrove salinity.
Tipo: Journal article
Palavras-chave: Bacillus; Paenibacillus; Ecological behavior.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100025
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
Provedor de dados: 58
Autores: Sun,Xinyi; Hao,Long; Ma,Hua; Li,Tong; Zheng,Lan; Ma,Zhao; Zhai,Guoyin; Wang,Liqin; Gao,Shanglong;
Liu,Xiaonan; Jia,Mengshi; Jia,Le.
The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were
optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be,
precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual
yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of
EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ±
3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher
than that of butylated...
Tipo: Journal article
Palavras-chave: Pleurotus eryngii SI-02; Exopolysaccharide; Extraction; Antioxidant activity; In vitro.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
Identification, isolation and optimization of antifungal metabolites from the Streptomyces
Malachitofuscus ctf9
Provedor de dados: 58
Autores: Sajid,Imran; Shaaban,Khaled A.; Hasnain,Shahida.
An indigenous Streptomyces isolate CTF9, exhibiting promising antifungal activity against Mucor miehei and Candida albicans in
pre-screening studies, was investigated by cultivation in a 50-L fermenter and by subsequent isolation, purification, and structure
elucidation of the active metabolites. Based on the morphological, biochemical, and physiological characterization, as well as the
16S rRNA gene sequence, the isolate CTF9 was identified as Streptomyces malachitofuscus. Using a series of chromatographic
techniques, two pure compounds were isolated from the obtained extracts after the fermentation of the isolate CTF9. The isolated
compounds were identified as phenylacetic acid and indolyl-3-lactic acid by mass spectrometry (MS) and NMR analysis. The...
Tipo: Journal article
Palavras-chave: Phenylacetic acid; Indolyl-3-lactic acid; Indigenous Streptomyces sp. CTF9.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200024
Candida Glabrata Esophagitis: new case reports and management
Provedor de dados: 58
Autores: Macêdo,Danielle Patrícia Cerqueira; Silva,Vanessa Karina Alves da; Farias,Aline Mary de Almeida; Melo,Luciana
Resende Bandeira de; Wilheim,Ana Botler; Neves,Rejane Pereira.
Candida esophagitis (CE) is a common opportunistic infection in the immunocompromised host. C. glabrata is rarely cited as
agent of CE and has been underestimated due to lack of proper identification. In this study, two cases of C. glabrata esophagitis in
AIDS and chagasic patients are reported. Diagnosis of Candida species should be considered an important key for the ideal choice
of antifungal therapy against this mycosis.
Tipo: Journal article
Palavras-chave: Candida glabrata; Esophagitis; AIDS; Chagas disease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200015
Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction
Provedor de dados: 58
Autores: Sepúlveda,Lya Madureira; Malirat,Viviana; Bergmann,Ingrid E.; Mantilla,Anibal; Nascimento,Elmiro Rosendo do.
Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease
in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock
productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from
Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus
Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for
Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the
polymerase...
Tipo: Journal article
Palavras-chave: Polymerase Chain reaction; Vesicular Stomatitis; Molecular diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300022
Chronic infection of cystic fibrosis patient airways by a single clone of Burkholderia cepacia:
replacement of non-mucoid to mucoid morphotype
Provedor de dados: 58
Autores: Carvalho,Ana Paula D'Alincourt; Ventura,Grasiella Maria de Carvalho; Folescu,Tânia Wrobel; D'Amoed,Renato Farme;
Higa,Laurinda; Teixeira,Lucia Martins; Merquior,Vânia Lúcia Carreira; Marques,Elizabeth de Andrade.
Mucoid Burkholderia cepacia morphotype emerged within a nine year follow-up of a cystic fibrosis patient. Clinical data
suggested a linkage between the mucoid phenotype isolation and the deterioration of the patient's condition. Despite of the
phenotypic variation, molecular typing showed that the patient was chronically infected with B. cepacia complex isolates
belonging to a same genetic clone.
Tipo: Journal article
Palavras-chave: Cystic Fibrosis; Burkholderia cepacia complex; PFGE; RAPD-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500014
Structure of xanthan gum and cell ultrastructure at different times of alkali stress
Provedor de dados: 58
Autores: Luvielmo,Márcia de Mello; Borges,Caroline Dellinghausen; Toyama,Daniela de Oliveira; Vendruscolo,Claire Tondo;
Scamparini,Adilma Regina Pippa.
Abstract The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at
the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater
xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali
stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification
are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali
stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation,...
Tipo: Journal article
Palavras-chave: Xanthomonas campestris; Xanthan production; Alkali stress; Transmission microscopy; Scanning electron
microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100102
Composition of Extracellular Polymeric Substances (EPS) produced by Flavobacterium columnare
isolated from tropical fish in Brazil
Provedor de dados: 58
Autores: Sebastião,Fernanda de Alexandre; Pilarski,Fabiana; Lemos,Manoel Victor Franco.
Thirty nine isolates of Flavobacterium columnare from Brazilian fish farms had their carbohydrate composition of EPS evaluated
by high efficiency liquid chromatography, using the phenol-sulfuric acid method of EPS. The occurrence of capsules on F.
columnare cells was not directly related to biofilm formation, and the predominant monosaccharide is glucose.
Tipo: Journal article
Palavras-chave: Biofilm; Extracellular polymeric substances; Flavobacterium columnare; Fish.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300030
AFLP analysis of Xanthomonas axonopodis and X. arboricola strains used in xanthan production
studies reveal high levels of polymorphism
Provedor de dados: 58
Autores: Mayer,Laurí; Silva,Wladimir Padilha da; Moura,Andrea Bittencourt; Vendruscolo,Claire Tondo.
Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 14 strains of Xanthomonas
arboricola pv. pruni and seven strains of X. axonopodis pv. phaseoli, which are used in xanthan production studies. Relationships
identified by the AFLP profiles were assessed for xanthan production capacity, geographical location and host plant. Strains were
isolated from 10 different geographic regions in South and Southeast States in Brazil. Data were analyzed for genetic similarity
using the Dice coefficient and subjected to UPGMA cluster analysis. A total of 128 AFLP fragments were generated from four
primer combinations: EcoRI+C/MseI+0, EcoRI+A/MseI+0, EcoRI+G/MseI+T and EcoRI+G/MseI+A. Of these, 96.1% were
polymorphic. X. axonopodis...
Tipo: Journal article
Palavras-chave: DNA fingerprints; Genomic diversity; Pathovar phaseoli; Pathovar pruni.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300026
Polymerase chain reaction detection of adenovirus DNA sequences in human lymphocytes
Provedor de dados: 58
Autores: Araújo,Aufra A.; Yokosawa,Jonny; Durigon,Edison L.; Ventura,Armando M.
Human lymphoid cells frequently carry adenoviruses and DNA sequences have been identified in peripheral blood lymphocytes
by Southern blot and PCR, although these cells are not permissive for virus replication, suggesting persistence of the viral
genome. In order to investigate this phenomenon we screened non-symptomatic volunteers for adenovirus DNA presence and
E1A gene expression. DNA samples extracted from peripheral blood mononuclear cells of 51 volunteers were submitted to PCR
using primers for a conserved hexon sequence, followed by nested PCR. Adenovirus sequences were detected in 27 samples
(52.9%). After more than one year, new samples of these positive volunteers were analyzed and in 70.8% of the cases the result
was maintained. Since this could...
Tipo: Journal article
Palavras-chave: Adenoviral infection; Viral persistence; Lymphocytes; Nested PCR assay.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200017
Antibiotic susceptibility patterns and prevalence of group B Streptococcus isolated from pregnant
women in Misiones, Argentina
Provedor de dados: 58
Autores: Quiroga,M.; Pegels,E.; Oviedo,P.; Pereyra,E.; Vergara,M.
This study was performed to determine the susceptibility patterns and the colonization rate of Group B Streptococcus (GBS) in a
population of pregnant women. From January 2004 to December 2006, vaginal-rectal swabs were obtained from 1105 women
attending Dr. Ramón Madariaga Hospital, in Posadas, Misiones, Argentina. The carriage rate of GBS among pregnant women was
7.6%. A total of 62 GBS strains were randomly selected for in vitro susceptibility testing to penicillin G, ampicillin, tetracycline,
levofloxacin,
gatifloxacin,
ciprofloxacin,
quinupristin-dalfopristin,
linezolid,
vancomycin,
rifampicin,
trimethoprim-sulfametoxazol, nitrofurantoin, gentamicin, clindamycin and erythromycin, and determination of resistance
phenotypes. No resistance to penicillin,...
Tipo: Journal article
Palavras-chave: Group B Streptococcus; Antibiotic susceptibility; Prevalence; Pregnant women; Argentina.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200009
Fusarium culmorum affects expression of biofilm formation key genes in Bacillus subtilis
Provedor de dados: 58
Autores: Khezri,Maryam; Jouzani,Gholamreza Salehi; Ahmadzadeh,Masoud.
Abstract It is known that there is correlation between biofilm formation and antagonistic activities of Bacillus subtilis strains; but,
the mechanism of this correlation is not clear. So, the effect of the plant pathogen (Fusarium culmorum) on the biofilm formation
in a B. subtilis strain with high antagonistic and biofilm formation activities was studied. The expression of sinR and tasA genes
involved in the biofilm formation was studied in both single culture of bacterium (B) and co-culture with F. culmorum (FB) using
real-time PCR. The results revealed that the expression of the sinR gene in both B and FB conditions was continuously decreased
during the biofilm formation period and, after 24 h (B4 and FB4), it reached 1% and 0.3% at the planktonic phase...
Tipo: Journal article
Palavras-chave: Bacillus subtilis; Biofilm; Sinr; Tasa; Fusarium culmorum.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100047
Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
Provedor de dados: 58
Autores: Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F.
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria
monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O
(TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a
total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated
skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay
duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The
protocol was faster...
Tipo: Journal article
Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040
Bacterial and yeast counts in Brazilian commodities and spices
Provedor de dados: 58
Autores: Freire,Francisco das Chagas Oliveira; Offord,Lisa.
A total of thirteen genera of bacteria and two genera of yeasts were detected in surface sterilized and unsterilized Brazilian
commodities and spices such as cashew kernels, Brazil nut kernels, black and white pepper. The genus Bacillus with eight species
was by far the most common. The yeasts isolated were Pichia sp., P. guillermondii and Rhodotorula sp. Bacillus cereus,
Salmonella typhimurium and Staphylococcus aureus were detected in cashew and Brazil nut kernels.
Tipo: Journal article
Palavras-chave: Cashew kernels; Brazil nut kernels; Black and white pepper; Microbiology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200009
Genetic analysis of mecA gene and detection of homologue pbpD in Stahylococcus sciuri group
Provedor de dados: 58
Autores: Calazans-Silva,Amanda C.; Medeiros,Pedro T.C.; Araujo,Dayanne M.; Carvalho,Bruno O.; Coelho,Irene S.;
Coelho,Shana M.O.; Souza,Miliane M.S.
Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown,
although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in
Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal
swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42)
and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for
pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented
phenotypic...
Tipo: Journal article
Palavras-chave: Staphylococcus sciuri; MecA; PbpD; Antimicrobial resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200038
Mechanism of infection and colonization of Rhipicephalus sanguineus eggs by Mertarhizium
anisopliae as revealed by scanning eletron microscopy and histopathology
Provedor de dados: 58
Autores: Garcia,Marcos Valerio; Monteiro,Antonio Carlos; Szabo,Matias Juan Pablo; Prette,Nancy; Bechara,Gervásio Henrique.
The present work aimed to verify the penetration pattern of the Metarhizium anisopliae fungus into Rhipicephalus sanguineus tick
eggs, as well as the lesions occurred inside the egg. The fungus adherence and penetration were studied by scanning electron
microscopy and the action of fungus in the internal tissues was evaluated through conventional histological sessions. For the
observation of these events, experimental infections were performed in 11 groups of R. sanguineus eggs containing 25 mg each.
The eggs were bathed during 3 minutes under manual shaking in a 10(8) conidia/mL suspension. The bath was performed only in
the suspension vehicle for the control groups. The eggs were processed for histopathological analysis and scanning electron
microscopy...
Tipo: Journal article
Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Ticks.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400012
Microbiological analyses of water from hemodialysis services in São Luís, Maranhão, Brazil
Provedor de dados: 58
Autores: Lima,José de Ribamar Oliveira; Marques,Sirley Garcia; Gonçalves,Azizedite Guedes; Salgado Filho,Natalino;
Nunes,Paulo Cruz; Silva,Hernandes Sousa; Monteiro,Silvio Gomes; Costa,Jackson Mauricio Lopes.
Rigorous control of water quality in hemodialysis services is extremely important in order to guarantee a better quality of life of
the patients submitted to this treatment. The lack of adequate water monitoring has caused the death of various patients in the past.
The objective of the present study was to determine the physicochemical and bacteriological characteristics of water used by
hemodialysis services in hospitals of the city of São Luís, Maranhão, Brazil. Bacteriological analyses included the membrane filter
method for the determination of total coliform bacteria, the Cult-Dipcombi-TTC-agar method for heterotrophic bacteria and the
limulus amebocyte lysate method for the determination of endotoxins. Eighteen water samples obtained from three...
Tipo: Journal article
Palavras-chave: Water; Microbiology; Hemodialysis; Contamination.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200001
Lectin-binding properties of Aeromonas caviae strains
Provedor de dados: 58
Autores: Rocha-de-Souza,Cláudio M.; Hirata-Jr,Raphael; Mattos-Guaraldi,Ana L.; Freitas-Almeida,Angela C.; Andrade,Arnaldo
F. B.
The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays
employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the
sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in
different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and
Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus
(EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding
lectin) in AeC398...
Tipo: Journal article
Palavras-chave: Aeromonas caviae; Lectin; Carbohydrate; Cell surface.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200003
Antibiotic resistance genes detected in the marine sponge Petromica citrina from Brazilian coast
Provedor de dados: 58
Autores: Laport,Marinella Silva; Pontes,Paula Veronesi Marinho; Santos,Daniela Silva dos; Santos-Gandelman,Juliana de
Fátima; Muricy,Guilherme; Bauwens,Mathieu; Giambiagi-deMarval,Marcia; George,Isabelle.
ABSTRACT Although antibiotic-resistant pathogens pose a significant threat to human health, the environmental reservoirs of the
resistance determinants are still poorly understood. This study reports the detection of resistance genes (ermB, mecA, mupA,
qnrA, qnrB and tetL) to antibiotics among certain culturable and unculturable bacteria associated with the marine sponge
Petromica citrina. The antimicrobial activities elicited by P. citrina and its associated bacteria are also described. The results
indicate that the marine environment could play an important role in the development of antibiotic resistance and the
dissemination of resistance genes among bacteria.
Tipo: Journal article
Palavras-chave: Microbes; Natural hotspots; Porifera; Resistance; Rio de Janeiro.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300617
HIV-1 subtypes and mutations associated to antiretroviral drug resistance in human isolates from
Central Brazil
Provedor de dados: 58
Autores: Cerqueira,Daniela Marreco; Ramalho,Eduardo Dias; Oliveira,Claudiner Pereira; Silva,Ruiter Roberto;
Franchini,Miriam; Felipe,Maria Sueli Soares; Martins,Cláudia Renata Fernandes.
The detection of polymorphisms associated to HIV-1 drug-resistance and genetic subtypes is important for the control and
treatment of HIV-1 disease. Drug pressure selects resistant variants that carry mutations in the viral reverse transcriptase (RT) and
protease (PR) genes. For a contribution to the public health authorities in planning the availability of therapeutic treatment, we
therefore described the genetic variability, the prevalence of mutations associated to drug resistance and the antiretroviral
resistance profile in HIV-1 isolates from infected individuals in Central Brazil. Nineteen HIV-1 RNA samples from a Public
Health Laboratory of the Federal District were reversely transcribed and cDNAs were amplified by nested PCR. One fragment of
297 bp...
Tipo: Journal article
Palavras-chave: HIV-1; Drug resistance; Antiretroviral drugs.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200003
Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis
Provedor de dados: 58
Autores: Tigano,Myrian Silvana; Aljanabi,Salah; Mello,Sueli Corrêa Marques de.
The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied
by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities
computed from RAPD markers showed two distinct genetic groups of isolates related to both species. RAPD analysis proved to be
an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in S. obtusifolia, and also for
distinguishing Alternaria species.
Tipo: Journal article
Palavras-chave: Fungi; Senna obtusifolia; Bioherbicide; Microbial control.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200005
Production and purification of an Endo–1,4-beta-Xylanase from Humicola grisea var.
thermoidea by electroelution
Provedor de dados: 58
Autores: Monti,Rubens; Cardello,Leonardo; Custódio,Marcos F.; Goulart,Antonio J.; Sayama,Adriana H.; Contiero,Jonas.
Humicola grisea var. thermoidea produces two forms of extracellular xylanase. The component form 1 was purified using the
electroelution method, due to the very small production of this extracellular enzyme. The apparent molecular mass was 61.8 kDa
by SDS-PAGE.
Tipo: Journal article
Palavras-chave: Electroelution; Enzyme purification; Extracellular xylanase; Humicola grisea var. thermoidea.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200007
In vitro inhibition of the bovine viral diarrhoea virus by the essential oil of Ocimum basilicum (basil)
and monoterpenes
Provedor de dados: 58
Autores: Kubiça,Thaís F.; Alves,Sydney H.; Weiblen,Rudi; Lovato,Luciane T.
The bovine viral diarrhoea virus (BVDV) is suggested as a model for antiviral studies of the hepatitis C virus (HCV). The antiviral
activity of the essential oil of Ocimum basilicum and the monoterpenes camphor, thymol and 1,8-cineole against BVDV was
investigated.
The
cytotoxicities
of
the
compounds
were
measured
by
the
MTT
(3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) test, and the antiviral activities were tested by the plaque
reduction assay. The oil or compounds were added to the assay in three different time points: a) pre-treatment of the virus
(virucidal assay); b) pre-treatment of the cells; or c) post-treatment of the cells (after virus inoculation). The percentage of plaques
inhibition for each compound was determined based on...
Tipo: Journal article
Palavras-chave: Antiviral; Camphor; 1.8-cineol; Thymol.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100028
Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene
Provedor de dados: 58
Autores: Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger;
Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry
was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S.
Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA),
sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by
contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and
scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both
materials...
Tipo: Journal article
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022
Pectinase production by Penicillium viridicatum RFC3 by solid state fermentation using agricultural
wastes and agro-industrial by-products
Provedor de dados: 58
Autores: Silva,Dênis; Martins,Eduardo da Silva; Silva,Roberto da; Gomes,Eleni.
Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out by means of
solid state fermentation using orange bagasse, corn tegument, wheat bran and mango and banana peels as carbon sources. The
maximal activity value of polygalacturonase (Pg) (30U.g-1) was obtained using wheat bran as carbon source while maximal
pectin lyase (Pl) (2000 U.g-1) activity value was obtained in medium composed of orange bagasse. Mixtures of banana or mango
peels with sugar cane bagasse resulted in increased Pg and Pl production compared to fermentations in which this residue was not
used. The mixture of orange bagasse and wheat bran (50%) increased the production of Pg and Pl to 55 U.g-1 and 3540 U.g -1
respectively....
Tipo: Journal article
Palavras-chave: Penicillium viridicatum polygalacturonase pectin lyase wastes; By-product.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400008
Virulence factors and biofilm production by isolates of Bacteroides fragilis recovered from dog
intestinal tracts
Provedor de dados: 58
Autores: Reis,Ana Catarina M.; Silva,Janice O.; Laranjeira,Bruno J.; Pinheiro,Adriana Q.; Carvalho,Cibele B.M.
Bacteroides fragilis colonizes dog guts both as a commensal and as an opportunistic pathogen. This study aims to evaluate
virulence factors of 13 B. fragilis strains isolated from dog intestinal tracts and their ability for biofilm formation. Capsules were
detected in all the evaluated strains. A total of 61.5% of all strains were biofilm producers. These attributes most likely play an
important role in B. fragilis persistent colonization in the gut.
Tipo: Journal article
Palavras-chave: B. fragilis; Biofilm; Virulence factors; Dogs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200037
Isolation of Escherichia coli and Salmonella spp. from free-ranging wild animals
Provedor de dados: 58
Autores: Iovine,Renata de Oliveira; Dejuste,Catia; Miranda,Flávia; Filoni,Claudia; Bueno,Marina Galvão; Carvalho,Vania Maria
de.
Increasing interactions between humans, domestic animals and wildlife may result in inter-species transmission of infectious
agents. To evaluate the presence of pathogenic E. coli and Salmonella spp. and to test the antimicrobial susceptibility of isolates,
rectal swabs from 36 different free-ranging wild mammals were taken from two distinct natural sites in Brazil: Cantareira State
Park (CSP, state of São Paulo) and Santa Isabel do Rio Negro Region (SIRNR, state of Amazonas). The swabs were randomly
collected and processed for bacterial isolation, identification, characterization and antimicrobial resistance. Eighteen E. coli strains
from CSP and 20 from SIRNR were recovered from 14 and 22 individuals, respectively. Strains from animals captured in CSP,...
Tipo: Journal article
Palavras-chave: E. coli; Salmonella; Wild mammals; One Health; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401257
Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome
broiler carcasses
Provedor de dados: 58
Autores: Moreira,Maria Aparecida S.; Rodrigues,Patrícia P.C.F.; Tomaz,Rafael S.; Moraes,Célia A. de.
Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important
disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches.
Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the
presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force
(PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and
AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel
Electrophoresis (PFGE) of DNA digested with...
Tipo: Journal article
Palavras-chave: Multiresistance; Antimicrobials; Enterobacteriaceae; Proton-motive force; Diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200007
Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus
Provedor de dados: 58
Autores: Abdallah,Fethi Ben; Chaieb,Kamel; Zmantar,Tarek; Kallel,Hela; Bakhrouf,Amina.
In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains,
food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and
Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black
colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V.
parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells
(OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all
the studied strains...
Tipo: Journal article
Palavras-chave: Vibrio; Slime production; Adherence; Abiotic surface; Vero cells.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200033
Kluyveromyces aestuarii, a potential environmental quality indicator yeast for mangroves in the State
of Rio de Janeiro, Brazil
Provedor de dados: 58
Autores: Araujo,F.V; Hagler,A. N.
Kluyveromyces aestuarii was found in sediments from 7 of 8 mangroves in Rio de Janeiro; and absent only at one site with heavy
plastic bag pollution. Its presence suggests influence in other habitats from a mangrove and its absence in a mangrove suggests
some non-fecal pollution or other habitat alteration.
Tipo: Journal article
Palavras-chave: Kluyveromyces aestuarii; Mangroves; Environmental indicator.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300014
Elimination of coliforms and Salmonella spp. in sheep meat by gamma irradiation treatment
Provedor de dados: 58
Autores: Henriques,Luciana Salles Vasconcelos; Henry,Fábio da Costa; Barbosa,João Batista; Ladeira,Silvania Alves;
Pereira,Silvia Menezes de Faria; Antonio,Isabela Maria da Silva; Teixeira,Gina Nunes; Martins,Meire Lelis Leal; Vital,Helio de
Carvalho; Rodrigues,Dália dos Prazeres; Reis,Eliane Moura Falavina dos.
This study aimed at evaluating the bacteriological effects of the treatment of sheep meat contaminated with total coliforms,
coliforms at 45 °C and Salmonella spp. by using irradiation at doses of 3 kGy and 5 kGy. Thirty sheep meat samples were
collected from animals located in Rio de Janeiro State, Brazil, and then grouped in three lots including 10 samples: non-irradiated
(control); irradiated with 3 kGy; and irradiated with 5 kGy. Exposure to gamma radiation in a 137Cs source-driven irradiating
facility was perfomed at the Nuclear Defense Section of the Brazilian Army Technological Center (CTEx) in Rio de Janeiro. The
samples were kept under freezing temperature (-18 °C) until the analyses, which occurred in two and four months after irradiation.
The...
Tipo: Journal article
Palavras-chave: Food irradiation; Microbiology; Sheep meat quality.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400017
Investigation of influenza in migrating birds, the primordial reservoir and transmitters of influenza in
Brazil
Provedor de dados: 58
Autores: Kawamoto,Adélia Hiroko Nagamori; Mancini,Dalva Assunção Portari; Pereira,Luiz Eloy; Cianciarullo,Aurora
Marques; Cruz,Aurea Silveira; Dias,Andrea Luppi Fernandes; Mendonça,Rita Maria Zucatelli; Pinto,José Ricardo;
Durigon,Edison Luiz.
Birds are the most important reservoirs of the influenza virus. Its maintenance in its natural hosts, including man, allows the
influenza virus to reassorts its strains. The recent report of an avian influenza A (H5N1) virus in humans, was in a child with fatal
respiratory illness in China, 1997. The current study was conducted to elucidate the transportation of the influenza by birds that
migrate, annually, through the both Northern and Southern hemispheres, with special attention paid to the Vireo olivaceus
[Juruviara(BR) or Red-eyed vireo(USA)] species, which travels from the USA to Brazil, and vice versa, and the Elaenia
mesoleuca [Tuque(BR) or (USA)] species that flies over the entire Southern Hemisphere. There are two species of birds, which
breed...
Tipo: Journal article
Palavras-chave: Migrating birds; Brazilian avian influenza virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100017
Development of a transformation system for Penicillium brevicompactum based on the Fusarium
oxysporum nitrate reductase gene
Provedor de dados: 58
Autores: Varavallo,Maurílio Antônio; Queiroz,Marisa Vieira de; Pereira,Jorge Fernando; Ribeiro,Ronney Adriano;
Soares,Marcos Antônio; Ribeiro,João Batista; Araújo,Elza Fernandes de.
Penicillium brevicompactum is a filamentous fungus that presents a potential for industrial use due its efficient pectinase
production. A heterologous transformation system was developed for P. brevicompactum based on the complementation of a
nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to
other mutations that lead to the chlorate resistance. One mutant named 4457-18X was chosen for the transformation experiments
with the pNH24 vector containing de Fusarium oxysporum nitrate reductase gene. A frequency of approximately 3
transformants/µg DNA was obtained using the circular vector pNH24. This frequency was multiplied about 10 fold using the
linearized vector with the Xba I...
Tipo: Journal article
Palavras-chave: Nitrate reductase; Penicillium brevicompactum; Heterologous transformation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200015
Isolation of Microsporum gypseum from the haircoat of health wild felids kept in captivity in Brazil
Provedor de dados: 58
Autores: Bentubo,Henri Donnarumma Levy; Fedullo,José Daniel Luzes; Corrêa,Sandra Helena Ramiro; Teixeira,Rodrigo
Hidalgo Friciello; Coutinho,Selene Dall' Acqua.
Dermatophytes are fungi that cause superficial mycoses in animals and humans. While studies have shown that domestic cats
(Felis catus) are often asymptomatic carriers of dermatophytes, and thus a significant source of infection, this aspect has not been
studied in relation to their wild relatives. The present study was aimed at determining the presence of dermatophytes on the
haircoat of healthy wild felids, kept in captivity at "Fundação Parque Zoológico de São Paulo". Samples were taken from 130
adult animals of both sexes: 25 lions (Panthera leo), 12 tigers (Panthera tigris), 6 jaguars (Panthera onca), 4 leopards (Panthera
pardus), 2 snow leopards (Panthera uncia), 2 pumas (Puma concolor), 2 cheetahs (Acinonyx jubatus), 1 ocelot (Leopardus
pardalis),...
Tipo: Journal article
Palavras-chave: Dermatophytes; Microsporum gypseum; Dermatophytosis; Wild felids.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200010
Viability and infectivity of an ectomycorrhizal inoculum produced in an airlift bioreactor and
immobilized in calcium alginate
Provedor de dados: 58
Autores: Oliveira,Leyza Paloschi de; Rossi,Márcio José; Furigo Júnior,Agenor; Silva Filho,Germano Nunes; Oliveira,Vetúria
Lopes de.
The viability and infectivity of an ectomycorrhizal inoculum (isolate UFSC-Rh90, Rhizopogon nigrescens), produced by
submerged cultivation in an airlift bioreactor and immobilized in beads of calcium alginate gel, was studied. Inoculum remained
100% viable after 18 months in a 0.85% NaCl solution at 8 ± 1ºC. Mycelium grew from the beads after 48 h when they were
placed on a solid culture medium at 25 ± 1ºC. Viability of pellets of non-immobilized mycelium stored under the same conditions
decreased gradually after the third month of storage, reaching 0% by the 12th month. These pellets presented a gradual darkening,
which was more intense in those located near the surface of the NaCl solution. In culture medium, these dark pellets showed no
viability. Gel...
Tipo: Journal article
Palavras-chave: Rhizopogon nigrescens; Ectomycorrhizal fungus; Submerged cultivation; Pinus taeda.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300011
Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in
chicken carcasses and pork meat
Provedor de dados: 58
Autores: Franchin,Paulo R.; Ogliari,Paulo J.; Andrade,Dalton F.; Chiapinoto,Maura; Lemos,Giovana; Rebelatto,Marina;
Silva,Ivair G. da; Batista,Cleide R.V.
A study was performed to compare the analytical procedure of the BAX® System for Salmonella PCR assay with the Modified
Semi-Solid Rappaport-Vassiliadis (MSRV) method, for the detection of Salmonella in naturally contaminated chicken carcass
samples (n = 762) and raw pork meat (n = 566). The chicken carcasses samples were collected during slaughtering after
defeathering or immediately after evisceration and the raw pork meat collected from the deboned head of recently slaughtered pigs
and others deboned raw fresh pork meat. The BAX® System detected 134 Salmonella-positive samples in chicken carcasses and
145 samples in pork meat, while the MSRV method isolated 142 and 144 Salmonella-positive samples, respectively. No
significant difference was observed...
Tipo: Journal article
Palavras-chave: BAX® System; MSRV method; Salmonella; Chicken; Pork meat.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400022
L-(+)-Lactic acid production by Lactobacillus rhamnosus B103 from dairy industry waste
Provedor de dados: 58
Autores: Bernardo,Marcela Piassi; Coelho,Luciana Fontes; Sass,Daiane Cristina; Contiero,Jonas.
ABSTRACT Lactic acid, which can be obtained through fermentation, is an interesting compound because it can be utilized in
different fields, such as in the food, pharmaceutical and chemical industries as a bio-based molecule for bio-refinery. In addition,
lactic acid has recently gained more interest due to the possibility of manufacturing poly(lactic acid), a green polymer that can
replace petroleum-derived plastics and be applied in medicine for the regeneration of tissues and in sutures, repairs and implants.
One of the great advantages of fermentation is the possibility of using agribusiness wastes to obtain optically pure lactic acid. The
conventional batch process of fermentation has some disadvantages such as inhibition by the substrate or the final...
Tipo: Journal article
Palavras-chave: Feed strategies; Lactic acid production; Lactobacillus and lactic acid purification.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300640
Persistence of Arcobacter butzleri CCUG 30484 on plastic, stainless steel and glass surfaces
Provedor de dados: 58
Autores: Cervenka,Libor; Kristlova,Jana; Peskova,Iva; Vytrasova,Jarmila; Pejchalova,Marcela; Brozkova,Iveta.
The persistence of A. butzleri CCUG 30484 on various surfaces under 32% and 64% relative humidity suspended in physiological
saline or nutrient broth to simulate relatively clean or soiled conditions was studied using various isolation techniques. Our study
revealed that A. butzleri CCUG 30484 cells were able to survive for a considerable period of time, even after the droplet of
suspending medium has been visibly dried. An extended survival on polypropylene coupons at both humidity levels was observed,
particularly at soiled conditions.
Tipo: Journal article
Palavras-chave: Arcobacter butzleri; Surfaces; Persistence.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300021
Influenza virus surveillance among young children in São Paulo, Brazil: the impact of vaccination
Provedor de dados: 58
Autores: Cabeça,Tatiane Karen; Watanabe,Aripuanã; Moreira,Luciana Peniche; Melchior,Thaís Boim; Perosa,Ana Helena;
Camargo,Clarice; Parmezan,Sheila Negrini; Bellei,Nancy.
This study assessed the presence of influenza virus among young children and the coverage of vaccination from 2010 to 2012 in
São Paulo, Brazil. Our results demonstrated a lower rate of influenza detection and a predominance of influenza B. A decrease of
coverage vaccination through the surveillance periods was observed.
Tipo: Journal article
Palavras-chave: Influenza virus; Surveillance; Vaccination.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300047
A survey of the bacterial diversity in the cup filler of dental chair units
Provedor de dados: 58
Autores: Silva,Vítor; Figueira,Vânia; Figueiral,Helena; Manaia,Célia M.
Water from the cup filler of dental chair units (CFDC) was observed to contain sphingomonads, environmental mycobacteria and
methylobacteria, among other minor bacteria. Some of the bacteria detected are recognized opportunistic pathogens. Some of
these, tended to persist over time.
Tipo: Journal article
Palavras-chave: Dental unit water; Water safety; Bacterial diversity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300015
Isolation and cultivation of fungal strains from in vitro cell cultures of two marine sponges (Porifera:
Halichondrida and Haplosclerida)
Provedor de dados: 58
Autores: Rozas,Enrique E.; Albano,Rodolpho M.; Lôbo-Hajdu,Gisele; Müller,Werner E.G.; Schröder,Heinz-C.; Custódio,Márcio
R.
Despite the large number of reports describing sponge-microbe associations, limited knowledge is available about associated fungi
and their relationships with the hosts. In this work, specific fungal strains were obtained directly from in vitro sponge cell cultures
(primmorphs) and single sponge cells (cytospins) and compared with those obtained from whole tissue preparations. A total of 27
fungal strains were isolated from the marine sponges Hymeniacidon heliophila and Haliclona melana. Fifteen strains, nine from H.
heliophila and six from H. melana, were obtained from whole tissue and were considered as possible mesohyl associated or
transient fungi. Twelve strains were isolated from in vitro sponge cell cultures (primmorphs) and were, therefore,...
Tipo: Journal article
Palavras-chave: Marine sponges; Primmorphs; Cytospins; Fungal detection.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400043
Metabolic networks and bioenergetics of Aurantiochytrium sp. B-072 during storage lipid formation
Provedor de dados: 58
Autores: Chaisawang,Montri; Verduyn,Cornelis; Chauvatcharin,Somchai; Suphantharika,Manop.
Baffled shake flask cultivation of Aurantiochytrium sp. B-072 was carried out at in a glucose-monosodium glutamate mineral
medium at different C/N-ratios (30-165) with glucose fixed at 90 g/L. With increasing C/N-ratio, a modest increase in lipid
content (60 to 73 % w/w) was observed whereas fat-free biomass decreased but overall biomass showed little variation.
FA-profiles were not affected to a large extent by C/N-ratio and absolute docosahexaenoic (DHA)-levels fell in narrow range (5-6
g/L). However at C/N > 64 a rapid decrease in lipid synthetic rate and/or incomplete glucose utilization occurred. Glucose and
FA-fluxes based on fat-free biomass peaked at a C/N ratio of 56. This condition was chosen for calculation of the redox balance
(NAD(P)H) and...
Tipo: Journal article
Palavras-chave: Aurantiochytrium; C/N-ratio; Docosahexaenoic acid; Fatty acid flux; P/O ratio.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300047
Presence of Staphylococcus spp. and Candida spp. in the human oral cavity
Provedor de dados: 58
Autores: Martins,Clélia Aparecida de Paiva; Koga-Ito,Cristiane Yumi; Jorge,Antonio Olavo Cardoso.
The presence of yeasts and staphylococci in the oral cavity is important because they can act as supplementary microbiota and in
certain situations can cause oral or systemic diseases. The aim of this work was to study the prevalence of Candida spp. and
Staphylococcus spp. in the human oral cavity. Oral rinses were collected from sixty-eight individuals according to the technique
described by Samaranayake and MacFarlane and then cultured on Sabouraud medium supplemented with chloramphenicol and
Baird-Parker agar. After the incubation period, the microorganisms were isolated and identified through biochemical tests. The
data obtained were statistically analysed by ANOVA. Candida spp. were isolated from 61.76% of the examined individuals and C.
albicans was...
Tipo: Journal article
Palavras-chave: Staphylococcus; Candida; Phospholipase; Proteinase; Killer system; Oral cavity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300009
Methodology for fast evaluation of Bacillus thuringiensis crystal protein content
Provedor de dados: 58
Autores: Alves,Lúcia M. Carareto; Lemos,Manoel V. F.
The development of the production and use of Bacillus thuringiensis in Brazil at a commercial scale faces certain difficulties,
among them the establishment of efficient methodologies for the quantitation of toxic products to be commercialized. Presently,
the amount of toxin is given in percentage by analyzing the samples total protein content. Such methodology however, does not
measure the actual amount of active protein present in the product, since most strains express different endotoxin genes and might
even produce b-toxin. Since the various types of toxins exhibit different antigenic characteristics, this work has as objective the
utilization of fast immunological techniques to quantify the level of crystal protein. Crystal protein produced by a...
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Crystal protein; Immunological methods.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300012
Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase
production by Streptomyces malaysiensis AMT-3
Provedor de dados: 58
Autores: Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues.
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by
Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation
periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained
from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and
212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by
actinomycetes, using BSG and CSL as economically feasible substrates.
Tipo: Journal article
Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020
Nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis
Provedor de dados: 58
Autores: Macedo,Maíra Bidart; Groll,Andrea Von; Fissette,Krista; Palomino,Juan Carlos; Silva,Pedro Eduardo Almeida da;
Martin,Anandi.
We validated the nitrate reductase assay (NRA) for the detection of multidrug-resistant Mycobacterium tuberculosis (MDR-TB)
using sodium nitrate (NaNO3) in replacement of potassium nitrate (KNO3) as nitrate source. NaNO3 is cheaper than KNO3 and
has no restriction on use which facilitates the implementation of NRA to detect MDR-TB.
Tipo: Journal article
Palavras-chave: Nitrate reductase assay tuberculosis multidrug resistant; Sodium nitrate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300019
Reducing lactate secretion by ldhA Deletion in L-glutamate- producing strain Corynebacterium
glutamicum GDK-9
Provedor de dados: 58
Autores: Zhang,Dalong; Guan,Dan; Liang,Jingbo; Guo,Chunqian; Xie,Xixian; Zhang,Chenglin; Xu,Qingyang; Chen,Ning.
L-lactate is one of main byproducts excreted in to the fermentation medium. To improve L-glutamate production and reduce
L-lactate accumulation, L-lactate dehydrogenase-encoding gene ldhA was knocked out from L-glutamate producing strain
Corynebacterium glutamicum GDK-9, designated GDK-9ΔldhA. GDK-9ΔldhA produced approximately
10.1% more L-glutamate than the GDK-9, and yielded lower levels of such by-products as α-ketoglutarate,
L-lactate and L-alanine. Since dissolved oxygen (DO) is one of main factors affecting L-lactate formation during L-glutamate
fermentation, we investigated the effect of ldhA deletion from GDK-9 under different DO conditions. Under both
oxygen-deficient and high oxygen conditions, L-glutamate...
Tipo: Journal article
Palavras-chave: Corynebacterium glutamicum; L-glutamate; L-lactate dehydrogenase; Gene knockout; Fermentation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400044
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous
recombination in yeast
Provedor de dados: 58
Autores: Silva Jr.,J.V.J.; Arenhart,S.; Santos,H.F.; Almeida-Queiroz,S.R.; Silva,A.N.M.R.; Trevisol,I.M.; Bertani,G.R.;
Gil,L.H.V.G.
The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and
vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time,
however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation
and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous
recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br)
was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The
clones were transfected into chicken...
Tipo: Journal article
Palavras-chave: Infectious bursal disease virus; Reverse genetics; Yeast-based homologous recombination.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400054
Occurrence of methicillin resistant Staphylococcus aureus (MRSA) among clinical samples in
tehran-iran and its correlation with polymorphism of specific accessory gene regulator (AGR) groups
Provedor de dados: 58
Autores: Azimian,Amir; Najar-pirayeh,Shahin; Mirab-Samiee,Siamak; Naderi,Mahmood.
Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital and community
acquired infections. Virulence gene expression in Staphylococcus aureus is orchestrated by regulators such as the accessory gene
regulator (agr). Staphylococcal strains are divided into four major agr groups (agrI-IV) on the basis of agrD and agrC
polymorphisms. The purpose of this study was to define the prevalence of MRSA strains in appointed Tehran's hospitals and then
to define and compare the proportion of agr I, II, III, IV polymorphisms between MRSA and Methicillin Sensitive Staphylococcus
aureus (MSSA) strains. A total of 235 isolates were evaluated by conventional antibiotic susceptibility tests and PCR for agr and
mecA...
Tipo: Journal article
Palavras-chave: Methicillin Resistant Staphylococcus aureus; Agr; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200043
Assessment of the efficiency of SimPlate™ total plate count color indicator (TPC CI) to
quantify mesophilic aerobic microorganisms in pasteurized milk
Provedor de dados: 58
Autores: Nero,Luís Augusto; Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Santana,Elsa Helena Walter de; Pereira,Mykel
Steffani; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de.
The SimPlate™ TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM) in foods, based
on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk
samples (38 type A, 43 type B and 61 type C) collected in Londrina, PR. The standard plating method, using Plate Count Agar
(PCA) was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of
false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were
compared by simple correlation and mean variance analyses. The correlation (r) and mean variance values were 0.6811 and
0.7583 for the results...
Tipo: Journal article
Palavras-chave: Rapid methods; Milk; SimPlate.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100009
Sporulation of Metarhizium anisopliae var. Acridum and Beauveria bassiana on Rhammatocerus
schistocercoides under humid and dry conditions
Provedor de dados: 58
Autores: Magalhães,Bonifácio Peixoto; Goettel,Mark Stanislaw; Frazão,Heloisa da Silva.
The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper
Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R.
schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity.
Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.
Tipo: Journal article
Palavras-chave: Entomopathogenic fungi; Internal sporulation; Grasshoppers; Microbial control.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300002
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv.
campestris
Provedor de dados: 58
Autores: Jiang,Guo-Feng; Jiang,Bo-Le; Yang,Mei; Liu,San; Liu,Jiao; Liang,Xiao-Xia; Bai,Xian-Fang; Tang,Dong-Jie;
Lu,Guang-Tao; He,Yong-Qiang; Yu,Di-Qiu; Tang,Ji-Liang.
It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most
bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal
media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector
expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen
interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME,
MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most
efficient for expression...
Tipo: Journal article
Palavras-chave: Inducing medium; Type III effector secretion; Xanthomonas.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045
Aflatoxin M1 in milk by immunoaffinity column cleanup with TLC/HPLC determination
Provedor de dados: 58
Autores: Shundo,Luzia; Sabino,Myrna.
During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized
in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79
(73.8%) of milk samples, ranging from <0.02 to 0.26 mug/L.The samples were analyzed using an immunoaffinity column for
cleanup and a thin layer chromatography for determining AFM1. The parameters, such as recovery, repeatibility, detection and
quantification limit were evaluated to optimize this method (in-house). Based on spiked samples, the recovery values ranged from
85.83 to 73.86% at levels of 0.010-0.50 mug/L, respectively, and the relative standard deviation for repeatibility ranged from 7.73
to 2.08%....
Tipo: Journal article
Palavras-chave: Milk; Aflatoxin M1; Thin layer chromatography; Immunoaffinity column.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200013
Occurrence of Salmonella spp. and generic Escherichia coli on beef carcasses sampled at a brazilian
slaughterhouse
Provedor de dados: 58
Autores: Silva,Fabiana Fernanda Pacheco da; Horvath,Mariana Bandeira; Silveira,Juliana Guedes; Pieta,Luiza; Tondo,Eduardo
Cesar.
A total of 120 beef carcasses were analyzed during processing at a slaughterhouse in southern Brazil. The carcasses were sampled
by swab at three different steps of the slaughter line and then they were tested for Salmonella and E. coli. The Salmonella isolates
were also examined for antimicrobial susceptibility. Salmonella prevalence distribution was modeled and the probability of
contamination was simulated using @Risk program and 10,000 interactions. Results demonstrated that 4 beef carcasses (3.3%)
were positive for Salmonella only in the first point. The six isolates of Salmonella were classified: S. Newport (n = 3), S.
Saintpaul (n = 2) and S. Anatum (n = 1). No Salmonella strains exhibited resistance to any of the antimicrobials tested. As
expected,...
Tipo: Journal article
Palavras-chave: Salmonella; E. coli; Beef carcasses; Slaughter line; Antimicrobial susceptibility.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100004
The effectiveness of alcohol gel and other hand-cleansing agents against important nosocomial
pathogens
Provedor de dados: 58
Autores: Hernandes,Silvio Evandro Daniel; Mello,Aline Cláudia de; Sant'Ana,Janete Jordão; Soares,Vanessa Sarto;
Cassiolato,Valdir; Garcia,Lourdes Botelho; Cardoso,Celso Luíz.
We compared the effectiveness of alcohol gel with that of the traditional hand-cleansing agents in removing clinical strains of
Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas
aeruginosa, and Candida albicans from artificially contaminated hands. The fingertips of 6 volunteers were contaminated with
approximately 10(6) of microbial cells, and then were washed with: plain liquid soap, alcohol gel, 70% ethyl alcohol (by weight),
10% povidone-iodine liquid soap (PVP-I), and 4% chlorhexidine gluconate detergent. The experiments were performed using a
Latin square statistical design, with six 6 x 5 randomized blocks, and the results were estimated by ANOVA. The products
reduced from...
Tipo: Journal article
Palavras-chave: Alcohol gel; Hand-cleansing agents; Nosocomial pathogens.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100005
A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for
two-dimensional gel electrophoresis
Provedor de dados: 58
Autores: Kumar,Gavish; Shankar,Hari; Bisht,Deepa; Sharma,Prashant; Singhal,Neelja; Katoch,V.M.; Joshi,Beenu.
Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile
of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods
as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the
preparation of sample from culture filtrate as well as other proteinaceous fluids.
Tipo: Journal article
Palavras-chave: Culture filtrate proteins; M. tuberculosis; SDS-TCA procedure; Two-dimensional gel electrophoresis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005
Evaluation of a multiplex selective enrichment broth SEL for simultaneous detection of injured
Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes
Provedor de dados: 58
Autores: Suo,Biao; Wang,Yuexia.
Although many rapid and high throughput molecular methods have been developed in the recent years for the multiplex detection
of foodborne pathogens, the simultaneous recovery and enrichment of sublethally injured cells is still a problem that needs to be
considered. Combined with previous established multiplex real-time PCR assay, the capability of simultaneous recovery and
enrichment of sublethally injured Salmonella, E. coli O157:H7 and L. monocytogenes cells was evaluated in a multiplex selective
enrichment broth SEL. The injured cells were obtained by heat shock. After evaluation of different procedures, 1 h of recovery
period prior to 20 h of enrichment was proved to be necessary for the detection of less than 10 CFU/5 mL broth of injured L....
Tipo: Journal article
Palavras-chave: Sublethal injury; SEL broth; Multiplex detection; Foodborne pathogen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300011
Adhesion of Salmonella Enteritidis to stainless steel surfaces
Provedor de dados: 58
Autores: Oliveira,Kelly; Oliveira,Tereza; Teixeira,Pilar; Azeredo,Joana; Oliveira,Rosário.
Adhesion of microorganisms to food processing surfaces and the problems it causes are a matter of strong concern to the food
industry. Contaminated food processing surfaces may act as potential sources of transmission of pathogens in food industry,
catering and in the domestic environments. Several studies have shown that adhesion of bacteria to surfaces partly depends upon
the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion
of four different strains of Salmonella Enteritidis to stainless steel 304 (SS 304). The effect of surface hydrophobicity and surface
elemental composition on the adhesion process was also analysed. Hydrophobicity was evaluated through contact angle...
Tipo: Journal article
Palavras-chave: Adhesion; Salmonella Enteritidis; Hydrophobicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200026
First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina
Provedor de dados: 58
Autores: Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.;
Romero,J.R.
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina.
IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311
PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP)
analysis.
Tipo: Journal article
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037
Oral administration of piperine for the control of aflatoxin intoxication in rats
Provedor de dados: 58
Autores: Gagini,Thalita B.; Silva,Robson E.; Castro,Isabela S.; Soares,Breno A.; Lima,Marco E.F.; Brito,Marilene F.;
Mazur,Carlos; Direito,Glória M.; Danelli,Maria das Graças M.
Aflatoxins are mycotoxins that have important toxic effects on human and animal health, even if consumed at low doses. The oral
administration of piperine (1.12 mg/kg) during 23 days in rats seemingly interfered with the toxicity of aflatoxins, decreasing
hepatic injuries and the leukocyte depletion in experimentally intoxicated animals.
Tipo: Journal article
Palavras-chave: Aflatoxins; Piperine; Experimental intoxication; Rats.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200013
Microbial activity, arbuscular mycorrhizal fungi and inoculation of woody plants in lead
contaminated soil
Provedor de dados: 58
Autores: Gattai,Graziella S; Pereira,Sônia V; Costa,Cynthia M. C; Lima,Cláudia E. P; Maia,Leonor C.
The goals of this study were to evaluate the microbial activity, arbuscular mycorrhizal fungi and inoculation of woody plants
(Caesalpinia ferrea, Mimosa tenuiflora and Erythrina velutina) in lead contaminated soil from the semi-arid region of northeastern
of Brazil (Belo Jardim, Pernambuco). Dilutions were prepared by adding lead contaminated soil (270 mg Kg-1) to
uncontaminated soil (37 mg Pb Kg soil-1) in the proportions of 7.5%, 15%, and 30% (v:v). The increase of lead contamination in
the soil negatively influenced the amount of carbon in the microbial biomass of the samples from both the dry and rainy seasons
and the metabolic quotient only differed between the collection seasons in the 30% contaminated soil. The average value of the
acid phosphatase...
Tipo: Journal article
Palavras-chave: AMF; Contaminated soil; Heavy metals; Mycorrhizae; Semi-arid.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300004
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a)
Provedor de dados: 58
Autores: Spilki,Fernando R.; Franco,Ana C.; Rijsewijk,Frans A. M.; Weiblen,Rudi; Flores,Eduardo F.; Roehe,Paulo M.
This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion
in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gEwere studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European
BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses
penetration assays were performed. When single step growth curves were studied, no statistical differences were observed
between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher
titres at 11 hours...
Tipo: Journal article
Palavras-chave: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis; Differential vaccine; In vitro growth
characteristics; GE- recombinant; BHV-1.2a; Bovine herpesviruses.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017
Occurrence of Salmonella sp. and coagulase-positive staphylococci in raw eggs and Coalho cheese:
comparative study between two cities of Brazil's northeast
Provedor de dados: 58
Autores: Evêncio-Luz,L; Lima-Filho,J.V.; Evêncio-Neto,J.
Microbiological analyses of chicken eggs in Recife and Salvador have shown a high occurrence of Salmonella in the egg shells
and yolks. Likewise, the occurrence of Salmonella plus coagulase-positive staphylococci in Coalho cheese reached alarming
levels. The data revealed a significant risk of infections and intoxications from consuming these foods in the cities.
Tipo: Journal article
Palavras-chave: Salmonella; Staphylococcus; Coalho cheese; Eggs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400030
Keratinolytic bacteria isolated from feather waste
Provedor de dados: 58
Autores: Riffel,Alessandro; Brandelli,Adriano.
The aim of this study was to characterize keratinolytic bacteria isolated from feather waste. Four isolates were selected after
growth on solid medium with feather meal as sole carbon and nitrogen source and screened for proteolytic activity on milk agar
plates. Three isolates were Gram-negative (belonged to the genera Burkholderia, Chryseobacterium and Pseudomonas) and one
was Gram-positive (Microbacterium sp.). These bacteria grew on diverse keratin wastes such as feather meal, raw feathers,
chicken nails, hair and wool. Keratinase activity was detected during growth, but the complete degradation of these substrates was
not always achieved. The proteolytic character of crude enzymes was assessed using azokeratin and azocasein as substrates. The...
Tipo: Journal article
Palavras-chave: Feather; Keratin; Peptidase; Proteolysis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300036
Strains of Lentinula edodes suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of
vesicular stomatitis virus
Provedor de dados: 58
Autores: Sasaki,Selma H.; Linhares,Rosa E.C.; Nozawa,Carlos M.; Montalván,Ricardo; Paccola-Meirelles,Luzia D.
Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species
of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on
Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi
and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the
Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal
pathogenic virus and filamentous fungi.
Tipo: Journal article
Palavras-chave: Phytopathogenic fungi; Shiitake; Inhibition; Vesicular Stomatitis Virus (Alagoas).
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100012
Keratinolytic abilities of Micrococcus luteus from poultry waste
Provedor de dados: 58
Autores: Laba,Wojciech; Choinska,Anna; Rodziewicz,Anna; Piegza,Michal.
Keratinolytic microorganisms have become the subject of scientific interest due to their ability to biosynthesize specific
keratinases and their prospective application in keratinic waste management. Among several bacterial classes, actinobacteria
remain one of the most important sources of keratin-degrading strains, however members of the Micrococcaceae family are rarely
scrutinized in regard to their applicatory keratinolytic potential. The tested Micrococcus sp. B1pz isolate from poultry feather
waste was identified as M. luteus. The strain, grown in the medium with 1–2% chicken feathers and a yeast extract
supplement, produced keratinases of 32 KU and lower level of proteases, 6 PU. It was capable to effectively decompose feathers
or...
Tipo: Journal article
Palavras-chave: Micrococcus luteus; Keratinase; Keratin; Biodegradation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300691
Yeast diversity associated to sediments and water from two Colombian artificial lakes
Provedor de dados: 58
Autores: Silva-Bedoya,L.M.; Ramírez-Castrillón,M.; Osorio-Cadavid,E.
In Colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with
clinical importance. Sediments and water represent important habitats for the study of yeast diversity, especially for yeast species
with industrial, biotechnological, and bioremediation potential. The main purpose of this study was to identify and compare the
diversity of yeast species associated with sediment and water samples from two artificial lakes in Universidad del Valle
(Cali-Colombia). Yeast samplings were performed from fifteen sediment samples and ten water samples. Grouping of similar
isolates was initially based on colony and cell morphology, which was then complemented by micro/mini satellite primed PCR
banding...
Tipo: Journal article
Palavras-chave: Yeast diversity; Artificial lake; Molecular identification; MSP-PCR fingerprinting.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100019
Serological and genetic diversity amongst Salmonella strains isolated in a salami processing line
Provedor de dados: 58
Autores: Ribeiro,Vinicius B.; Andrigheto,Cristiano; Bersot,Luciano S.; Barcellos,Vinicius; Reis,Eliana F.; Destro,Maria T.
Salmonella is one of the most important agents of foodborne disease in Brazil and in other countries, with meat and meat products
being identified as important vehicles of salmonelosis. A total of 54 Salmonella strains isolated from a commercial salami
processing line were first serotyped and then their antibiotic resistance and macro restriction profiles were determined. 11.1% of
the strains showed resistance to 3 or more antibiotics with profile AmpCStxTe being the most frequent. PFGE generated 9 and 12
profiles with enzymes XbaI and SpeI, respectively. It was observed that different serotypes of Salmonella could be found in the
different steps of the processing line. The genetic profile of the strains had low relationship indicating the genetic...
Tipo: Journal article
Palavras-chave: Salmonella sp.; Salami; PFGE.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100036
Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr
markers
Provedor de dados: 58
Autores: Brasileiro,Bereneuza Tavares Ramos Valente; Coimbra,Maria Raquel Moura; Morais Jr,Marcos Antonio de;
Oliveira,Neiva Tinti de.
Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that
causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The
complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for
isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to
investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates.
Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical...
Tipo: Journal article
Palavras-chave: Intron splice site; Fusarium solani; Microsatellite; PCR-fingerprinting; Ribosomal DNA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state
fermentation using Aspergillus niger GH1
Provedor de dados: 58
Autores: Veana,F.; Martínez-Hernández,J.L.; Aguilar,C.N.; Rodríguez-Herrera,R.; Michelena,G.
Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and
sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an
important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican
semi-desert, previously reported as an invertase over-producer strain was used. Molasses from Mexico and Cuba were chemically
analyzed (total and reducer sugars, nitrogen and phosphorous contents); the last one was selected based on chemical composition.
Fermentations were performed using virgin and hydrolyzate bagasse (treatment with concentrated sulfuric acid). Results indicated
that,...
Tipo: Journal article
Palavras-chave: By-products virgin and hydrolyzated bagasse β -fructofuranosidase xerophilic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200002
Candida dubliniensis in a Brazilian family with an HIV 1- infected child: identification, antifungal
susceptibility, drug accumulation and sterol composition
Provedor de dados: 58
Autores: Melo,Nadja Rodrigues de; Taguchi,Hideaki; Culhari,Vitoria V.P.; Sano,Ayako; Fukushima,Kazutaka; Miyaji,Makoto;
Manning,Nigel; Kelly,Steven L.; Vilela,Marluce S.
This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV - infected child. A total of 42 oral
isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain
reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child
and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child
exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 µg/mL for voriconazole and fluconazole, respectively.
Accumulation of [³H] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to
the...
Tipo: Journal article
Palavras-chave: Candida dubliniensis; Antifungal agents; Sterol composition.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300008
Rickettsia felis infection in cat fleas Ctenocephalides felis felis
Provedor de dados: 58
Autores: Horta,Mauricio C.; Scott,Fabio B.; Correia,Thaís R.; Fernandes,Julio I.; Richtzenhain,Leonardo J.; Labruna,Marcelo B.
The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in
Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR)
were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial
sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R.
felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8
cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory,...
Tipo: Journal article
Palavras-chave: Rickettsia felis; Flea; Ctenocephalides felis felis; Cats; Humans.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300035
Patulin accumulation in apples during storage by Penicillium expansum and Penicillium griseofulvum
strains
Provedor de dados: 58
Autores: Welke,Juliane Elisa; Hoeltz,Michele; Dottori,Horacio Alberto; Noll,Isa Beatriz.
A part of apples destined to juice production is generally of poor quality. Apples from cold storage or recently harvest (ground
harvested or low quality apples) are stored under ambient conditions until they are processed. Since Penicillium expansum and P.
griseofulvum are the principal fungal species isolated from stored apples in Brazil, the objective of this study was to investigate
the ability of these strains to produce patulin in apples and report the consequences of this type of storage in loss of quality. The
toxin was quantified using thin layer chromatography and charge-coupled device camera (TLC-CCD). The rate and quantities that
P. expansum and P. griseofulvum can grow and produce patulin are highly dependent on the fungal strain and time....
Tipo: Journal article
Palavras-chave: Patulin; Apple; Penicillium expansum; Penicillium griseofulvum.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100021
Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans
Provedor de dados: 58
Autores: Barbieri,Dicler de Sant'Anna Vitor; Vicente,Vânia Aparecida; Fraiz,Fabian Calixto; Lavoranti,Osmir José;
Svidzinski,Terezinha Inês Estivalet; Pinheiro,Rosângela Lameira.
The objective of the present study was to investigate the in vitro adherence capacity of Streptococcus mutans and Candida
albicans. Adherence assays were conducted on dental surfaces and analyzed by scanning electron microscopy (SEM). Extracted
human teeth were inoculated with Streptococcus mutans or Candida albicans and with both species simultaneously, and incubated
at 37ºC for 21 days. Bacterial inocula had been obtained from saliva samples of children that had been colonized by both
organisms. ATCC reference strains were used as controls. SEM analyses showed that the biofilm that covered the entire analyzed
dental surface was more homogeneous inoculated with the two microorganisms simultaneously than with each species separately.
In a second...
Tipo: Journal article
Palavras-chave: Adherence; Candida albicans; Streptococcus mutans; Biofilm.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400009
Erratum vol. 32(1), 2001
Provedor de dados: 58
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200019
Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY
antibodies from ostrich egg yolk
Provedor de dados: 58
Autores: Tobias,Fernando Luiz; Garcia,Luize Néli Nunes; Kanashiro,Milton Masahiko; Medina-Acosta,Enrique;
Brom-de-Luna,João Gato; Almeida,Claudia Maria Costa de; Azevedo Junior,Romildo Rocha; Lemos,Môsar;
Vieira-da-Motta,Olney.
Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to
rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and
Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of
ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds
were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both
derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial
muscle. Two...
Tipo: Journal article
Palavras-chave: Ostrich IgY antibodies; Inhibition; Recombinant proteins; E. coli; S. aureus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200015
Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages
Provedor de dados: 58
Autores: Soleimani-Delfan,Abbas; Etemadifar,Zahra; Emtiazi,Giti; Bouzari,Majid.
One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium
causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of
restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate
the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from
infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii
strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated
from...
Tipo: Journal article
Palavras-chave: Dickeya dadantii; Soft rot; Bacteriophage; Antibiotic resistance; Biocontrol.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300791
Growth of Byssochlamys nivea in pineapple juice under the effect of water activity and ascospore age
Provedor de dados: 58
Autores: Zimmermann,M; Miorelli,S; Massaguer,P.R; Aragão,G.M.F.
The study of thermal resistant mould, including Byssochlamys nivea, is of extreme importance since it has been associated with
fruit and fruit products. The aim of this work is to analyze the influence of water activity (a w) and ascospore age (I) on the
growth of Byssochlamys nivea in pineapple juice. Mold growth was carried out under different conditions of water activity (a w)
(0.99, 0.96, 0.95, 0.93, 0.90) and ascospore age (I) (30, 51, 60, 69, 90 days). Growth parameters as length of adaptation pha se
(λ), maximum specific growth rate (µmax) and maximum diameter reached by the colony (λ) were
obtained through the fit of the Modified Gompertz model to experimental data (measuring radial colony diameter). Statistica 6.0
was used for...
Tipo: Journal article
Palavras-chave: Byssochlamys nivea; Mould growth; Pineapple juice; Water activity; Ascospore age.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100025
Viability of stressed Mycobacterium tuberculosis and association with multidrug resistance
Provedor de dados: 58
Autores: Martins,Maria Conceição; Giampaglia,Carmen Maria Saraiva; Chimara,Erica; Oliveira,Rosângela Siqueira;
Vedovello,Danielle; Sakamoto,Sidnei Miyoshi; Ferrazoli,Lucilaine.
This study investigated biological characteristics of recovered stressed M. tuberculosis isolates that failed to grow in differential
culture media for phenotypic identification and in culture media containing anti-tuberculosis drugs for drug-susceptibility testing,
despite of having grown in primary culture. It represents an improvement in the diagnosis of MDR tuberculosis and tuberculosis
control.
Tipo: Journal article
Palavras-chave: Mycobacterium; Multidrug resistance; Culture growth; Spoligotyping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200019
Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and
pathogenesis genes in response to 6°c, 65°c and ph 2.0
Provedor de dados: 58
Autores: Pribylova,Radka; Kralik,Petr; Donnelly,Neysan; Matiasovic,Jan; Pavlik,Ivo.
The aim of this work was to study the expression of selected Mycobacterium avium subsp. paratuberculosis (MAP) genes
connected with MAP virulence, adhesion and stress response. The temperature of 6°C and 65°C were chosen with regard to the
food industry, storage conditions (refrigerator) and low-temperature pasteurization. A pH of 2.0, using lactic acid, was selected to
mimic the natural environment of the stomach. Expression of selected genes was studied using real time reverse transcription PCR
on three different MAP isolates. MAP isolates were chosen according to the number of their preceding cultivations. While isolates
8672 and 8819 were previously cultivated only once, MAP isolate 12146 went through four passages. Different expression
profiles were...
Tipo: Journal article
Palavras-chave: MAP; Johne's disease; Stress; Treatment; Real time PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200049
Soil streptomycetes with in vitro activity against the yam pathogens Curvularia eragrostides and
Colletotrichum gloeosporioides
Provedor de dados: 58
Autores: Soares,Ana Cristina Fermino; Sousa,Carla da Silva; Garrido,Marlon da Silva; Perez,Jane Oliveira; Almeida,Nailson
Santos de.
Yam (Dioscorea cayennensis Lam), an important crop in the Northeastern region of Brazil, is susceptible to the attack of several
phytopathogenic fungi, such as Curvularia eragrostides (Henn.) Meyer and Colletotrichum gloeosporioides (Penz.), which cause
leaf spot diseases. This work aimed to evaluate the effect of six actinomycete isolates (S. thermotolerans, S. griseus subsp. griseus,
Streptomyces sp. N0035, S. purpurascens, and two isolates identified as Streptomyces sp.), as well as the effect of their secondary
metabolites, on mycelium growth and spore germination of C. eragrostides and C. gloeosporioides, from yam plants, cultivar da
Costa. Additionally, the chitinolytic activity of these actinomycete strains was determined in a minimum salt growth...
Tipo: Journal article
Palavras-chave: Biocontrol; Dioscorea cayennensis; Actinomycetes; Secondary metabolites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400010
Depuration Times of Donax trunculus and Tapes decussatus
Provedor de dados: 58
Autores: Çolakolu,Fatma Arik; Çardak,Mine; Çolakoglu,Serhat; Künili,Ibrahim Ender.
The present study was performed to determine the depuration time and ability of Donax trunculus (Wedge Clam) and Tapes
decussatus (Carpet Shell) contaminated with Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium and
Vibrio parahaemolyticus. Clams were contaminated with each bacterium at the level of 7.0 - 8.0 Log10 cfu/g. After
contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. During the
depuration process of both clams, the level of bacteria decreased quickly to 40% of initial load in the first 12 h. The results of this
study indicate that the depuration time of carpet shells for all bacteria is 66 h. The depuration process of the wedge clam was
different from the carpet...
Tipo: Journal article
Palavras-chave: Clam; Purification; Vibrio parahaemolyticus; Escherichia coli; Salmonella typhimurium.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300034
Occurrence and molecular characterization of Cucumber green mottle mosaic virus in cucurbit crops
of KPK, Pakistan
Provedor de dados: 58
Autores: Ali,Asad; Hussain,Adil; Ahmad,Musharaf.
Field survey of the cucurbit crops revealed a high incidence of Cucumber green mottle mosaic virus (CGMMV) in Khyber
Pakhtunkhwa Province (KPK), Pakistan. Among the seven districts surveyed, average percent incidence of CGMMV was
recorded up to 58.1% in district Nowshera, followed by 51.1% in district Charsada, 40.5% in district
Swabi and 37.3% in district Mardan. In Swat and Dir districts average incidence CGMMV was recorded upto
31.2% and 29.4%, respectively. Among the different crops highest incidence in plain areas of KPK was
recorded in bottle gourd (59.3%) followed by 56.3% in Squash, 54.5% in Pumpkin, 45.5% in
Melon, 41.7% in Cucumber and 29.9% in Sponge...
Tipo: Journal article
Palavras-chave: Cucurbit; CGMMV; Incidence; Tobamovirus; Pakistan.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400015
Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus
of breast-fed lamb
Provedor de dados: 58
Autores: Sepová,H. Kiňová; Dubničková,M; Bilková,A; Bukovský,M; Bezáková,L.
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA
fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune
system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus,
Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in
the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells
was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly
induced by...
Tipo: Journal article
Palavras-chave: Lactobacillus reuteri; Mononuclear cells; PCR; Antagonistic activity; Phagocytosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300044
Production and characterization of an enzyme complex from a new strain of Clostridium
thermocellum with emphasis on its xylanase activity
Provedor de dados: 58
Autores: Vieira,Werner Bessa; Moreira,Leonora Rios de Souza; Monteiro Neto,Amadeu; Ferreira Filho,Edivaldo Ximenes.
A new bacterial strain (ISO II) was isolated from manure cow and identified as phylogenetically close to the thermophilic
cellulolytic bacterium Clostridium thermocellum. The new strain produced extracellular xylanase, pectinase, mannanase and
cellulase activities when grown in liquid culture medium containing banana stem as carbon source. The enzyme production profile
after growth on banana stem showed that xylanase and cellulase activities were detected in different incubation periods. An
enzyme complex containing xylanase, cellulase and mannanase activities was isolated from culture supernatant samples of
strainISO II. The complex was partially purified by ultrafiltration and gel filtration chromatography on Sephacryl S-300.
Zymogram analysis after...
Tipo: Journal article
Palavras-chave: Clostridium thermocellum; Banana stem; Xylanase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200009
Evaluation of two recommended disinfection methods for cleaning cloths used in food services of
southern Brazil
Provedor de dados: 58
Autores: Bartz,Sabrina; Tondo,Eduardo Cesar.
In the State of Rio Grande do Sul (RS), Southern Brazil, a good manufacturing practices regulation was published recommending
two disinfection methods for cleaning cloths used in food services. The aim of the present study was to evaluate the efficacy of
those methods. Cleaning cloths were sampled without prior notice at food services, on common working days. For the analyses,
the cloths were divided in two sub-samples, being one of them microbiologically analyzed. The second sub-sample was further
divided in two pieces and submitted to hand washing for two minutes. After that, one piece was boiled in water for 15 min and the
other one was soaked in a 200 ppm sodium hypochlorite solution for 15 min. Both pieces of cloth were submitted to
microbiological...
Tipo: Journal article
Palavras-chave: Cleaning cloths; Disinfection; The State of Rio Grande do Sul; Brazilian food services.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300015
Distribution of non-LEE-encoded type 3 secretion system dependent effectors in enteropathogenic
Escherichia coli
Provedor de dados: 58
Autores: Salvador,Fábia A.; Hernandes,Rodrigo T.; Vieira,Mônica A.M.; Rockstroh,Anna C.; Gomes,Tânia A.T.
Enteropathogenic Escherichia coli (EPEC) are important human gastroenteritis agents. The prevalence of six non-LEE genes
encoding type 3 translocated effectors was investigated. The nleC, cif and nleB genes were more prevalent in typical than in
atypical EPEC, although a higher diversity of genes combinations was observed in atypical EPEC.
Tipo: Journal article
Palavras-chave: Enteropathogenic Escherichia coli (EPEC); Type 3 secretion system; Pathogenicity; Diarrhea.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300014
Multi drug resistance in strong biofilm forming clinical isolates of Staphylococcus epidermidis
Provedor de dados: 58
Autores: Sahal,Gulcan; Bilkay,Isil Seyis.
Staphylococcus epidermidis which exists in healthy human skin as a commensal inhabitant is also an important pathogen forming
biofilms on many surfaces and recently, increased resistance traits were suggested to be acquired in biofilm environments. In this
study; clinical Prevalences, antibiotic resistances and biofilm formations of S. epidermidis strains were determined and
comparison of all these findings with each other was carried out in order to take precautions against them and figure out if high
biofilm forming S. epidermidis strains display multi drug resistance. According to our results; samples of wound and blood were
the most S. epidermidis isolated clinical materials (40%; 35%) and cardiothoracic surgery was the most S. epidermidis observed...
Tipo: Journal article
Palavras-chave: Staphylococcus epidermidis biofilm formation β -lactam resistance multi drug resistance indwelling
medical device related infections.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200023
Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian
basidiomycetes fungi
Provedor de dados: 58
Autores: Machado,Kátia M. G.; Matheus,Dácio R.; Bononi,Vera L. R.
Remazol Brilliant Blue R (RBBR) dye was used as substrate to evaluate ligninolytic activity in 125 basidiomycetous fungi
isolated from tropical ecosystems. The extracellular RBBR decolorizing activity produced when selected fungi were grown in
solid media and in soil contaminated with organochlorines was also evaluated. A total of 106 fungi decolorized the RBBR during
the growth in malt extract agar (MEA, 2%); 96 fungi showed a mycelia growth and decolorization activity stronger than the P.
chrysosporium used as reference. Extracellular extracts of 35 selected fungi grown on solid medium with sugar cane bagasse
(BGS) were evaluated for RBBR decolorization and peroxidase activity. All fungi showed peroxidase activities, but 5 of those
were unable to...
Tipo: Journal article
Palavras-chave: White rot fungi; Peroxidases; Laccase; MnP; Pentachlorophenol; Hexachlorobenzene.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300008
Ethanol fermentation of sugarcane molasses by Zymomonas mobilis MTCC 92 immobilized in Luffa
cylindrica L. sponge discs and Ca-alginate matrices
Provedor de dados: 58
Autores: Behera,Shuvashish; Mohanty,Rama C.; Ray,Ramesh C.
Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis
MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end
of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and Ca-alginate
entrapped Z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. There was no statistical
significant difference (Fischer's LSD) in sugar utilization (t = 0.254, p <0.801) and ethanol production (t =-0.663, p <0.513)
between the two immobilization matrices used. Further, the immobilized cells in both the matrices were physiologically active
for...
Tipo: Journal article
Palavras-chave: Bio-ethanol; Cell immobilization; Fermentation; Molasses; Zymomonas mobilis MTCC 92.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400034
BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon
Basin, recognizing 5'-GAG¯CTC-3'
Provedor de dados: 58
Autores: Chies,Jocelei M.; Dias,Ana C. de O.; Maia,Hélio M.M.; Astolfi-Filho,Spartaco.
A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River.
Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the
native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined.
Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA
sequencing
of
restriction
fragments
allowed
the
unequivocal
demonstration
of
5´GAG<FONT
FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a
neoschizomer of the prototype restriction endonuclease SacI....
Tipo: Journal article
Palavras-chave: Type II restriction endonuclease; BpuAmI; SacI; Neoschizomers; Bacillus pumilus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100018
Healthcare-associated vancomycin resistant Enterococcus faecium infections in the Mansoura
University Hospitals intensive care units, Egypt
Provedor de dados: 58
Autores: Moemen,Dalia; Tawfeek,Doaa; Badawy,Wafaa.
Vancomycin resistant Enterococcus faecium (VREF) ia an emerging and challenging nosocomial pathogen. This study aimed to
determine the prevalence, risk factors and clonal relationships between different VREF isolates in the intensive care units (ICUs)
of the university hospitals in our geographic location. This prospective study was conducted from July, 2012 until September,
2013 on 781 patients who were admitted to the ICUs of the Mansoura University Hospitals (MUHs), and fulfilled the
healthcare-associated infection (HAI) criteria. Susceptibility testing was determined using the disk diffusion method. The clonal
relationships were evaluated with pulsed field gel electrophoresis (PFGE). Out of 52 E. faecium isolates, 12 (23.1%) were
vancomycin resistant....
Tipo: Journal article
Palavras-chave: Enterococcus faecium; ICU; Vancomycin; Risk factors; PFGE.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300777
Mucorales from the semiarid of Pernambuco, Brazil
Provedor de dados: 58
Autores: Santiago,André Luiz Cabral Monteiro de Azevedo; Santos,Paulo Jorge Parreira dos; Maia,Leonor Costa.
Nineteen taxa of Mucorales, belonging to Absidi, Apophysomyces, Cunninghamella, Fennellomyces, Lichtheimi, Mucor,
Mycotypha, Rhizopus and Syncephalastrum were isolated from 36 composite soil samples in three semiarid areas in the State of
Pernambuco (Triunfo, Cabrobó and Belém de São Francisco), Northeast Brazil, which are characterized by Caatinga vegetation.
Triunfo is preserved, whereas Cabroró and Belém de São Francisco are experiencing low and severe desertification processes,
respectively. Mucorales were isolated in Petri dishes in triplicate from 5 mg samples of soil placed on the surface of wheat germ
agar plus chloramphenicol and Cercobin [Dimethyl 4,49-(103 phenylene) bis (3-thioallophanate)] medium. The plates were left on
a bench at room...
Tipo: Journal article
Palavras-chave: Biodiversity; Caatinga; Mucoromycotina; Soil degradation; Taxonomy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100044
Ocurrence of blaSPM-1 and blaIMP-1 genes of metallo-beta-lactamases in clinical isolates of
Pseudomonas aeruginosa from three universitary hospitals in the city of Porto Alegre, Brazil
Provedor de dados: 58
Autores: Gaspareto,Patrick Barcelos; Martins,Andreza Francisco; Zavascki,Alexandre Prehn; Barth,Afonso Luis.
We described the ocurrence of metallo-beta-lactamases (MBL) genes blaSPM-1 and blaIMP-1 in clinical isolates of Pseudomonas
aeruginosa resistant to imipenem and/or ceftazidime obtained from three universitary hospitals in the city of Porto Alegre, Brazil.
The MBL production was screened by phenotypic test and the genes were detected by PCR.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Imipenem resistance; Metallo-beta-lactamase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100022
Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of
Pakistan and their industrial importance
Provedor de dados: 58
Autores: Akhtar,Nasrin; Ghauri,Muhammad A.; Iqbal,Aamira; Anwar,Munir A.; Akhtar,Kalsoom.
Culturable bacterial biodiversity and industrial importance of the isolates indigenous to Khewra salt mine, Pakistan was assessed.
PCR Amplification of 16S rDNA of isolates was carried out by using universal primers FD1 and rP1and products were sequenced
commercially. These gene sequences were compared with other gene sequences in the GenBank databases to find the closely
related sequences. The alignment of these sequences with sequences available from GenBank database was carried out to
construct a phylogenetic tree for these bacteria. These genes were deposited to GenBank and accession numbers were obtained.
Most of the isolates belonged to different species of genus Bacillus, sharing 92-99% 16S rDNA identity with the respective type
strain. Other...
Tipo: Journal article
Palavras-chave: Biodiversity; Characterization; Highly saline environments; PCR amplification of 16S rDNA; Industrially
important enzymes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100029
Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
Provedor de dados: 58
Autores: Monte,Leonardo Garcia; Jorge,Sérgio; Luiz,João Paulo Mesquita; Sinnott,Francine; Seixas,Fabiana Kömmling;
Aleixo,José Antonio Guimarães; Samartino,Luis Ernesto; Conceição,Fabricio Rochedo; Hartleben,Cláudia Pinho.
Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an
immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from
dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
Tipo: Journal article
Palavras-chave: Canine leptospirosis; IMS-PCR; LipL32.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023
Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates
Provedor de dados: 58
Autores: Mohamed,Yasmine Fathy; Abou-Shleib,Hamida Moustafa; Khalil,Amal Mohamed; El-Guink,Nadia Mohamed;
El-Nakeeb,Moustafa Ahmed.
Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the
medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in
this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those
pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug
resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and
construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was...
Tipo: Journal article
Palavras-chave: Pan-drug resistant; Leakage; Permeability alteration; Colistin; Ultrastructure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381
Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and
random amplified polymorphic DNA
Provedor de dados: 58
Autores: Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad
Mehdi.
In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA
(RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by
RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying
reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed
sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized
RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with
PFGE.
Tipo: Journal article
Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024
Dormancy models for Mycobacterium tuberculosis: A minireview
Provedor de dados: 58
Autores: Alnimr,Amani M.
Dormancy models for Mycobacterium tuberculosis play important roles in understanding various aspects of tuberculosis
pathogenesis and in the testing of novel therapeutic regimens. By simulating the latent tuberculosis infection, in which the bacteria
exist in a non-replicative state, the models demonstrate reduced susceptibility to antimycobacterial agents. This minireview
outlines the models available for simulating latent tuberculosis both in vitro and in several animal species. Additionally, this
minireview discusses the advantages and disadvantages of these models for investigating the bacterial subpopulations and
susceptibilities to sterilization by various antituberculosis drugs.
Tipo: Journal article
Palavras-chave: Dormancy; Latency; Tuberculosis; Stationary-phase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300641
Identification of bacterial agents of enteric diseases by multiplex PCR in growing-finishing pigs
Provedor de dados: 58
Autores: Baccaro,Maria Regina; Moreno,Andrea Micke; Shinya,Luciane Tieko; Dotto,Daniela Sabatini.
In Brazil, the most common bacterial enteric diseases affecting growing and finishing pigs are porcine proliferative enteritis,
porcine intestinal spirochetosis, swine dysentery, and salmonellosis. The diagnosis of these diseases by routine culture techniques
is expensive, difficult, time-consuming, and even impossible, in cases of porcine proliferative enteritis. The detection of pathogens
by polymerase chain reaction is a highly sensitive and specific method that can be an useful tool in veterinary diagnosis. Two
multiplex PCR (M-PCR) assays were tested for simultaneous detection and identification of bacterial agents associated with
porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis in diarrheic fecal...
Tipo: Journal article
Palavras-chave: Enteritis; PCR; Pig; Bacteria; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300008
Ochratoxigenic fungi associated with green coffee beans (Coffea arabica L.) in conventional and
organic cultivation in Brazil
Provedor de dados: 58
Autores: Rezende,Elisângela de Fátima; Borges,Josiane Gonçalves; Cirillo,Marcelo Ângelo; Prado,Guilherme; Paiva,Leandro
Carlos; Batista,Luís Roberto.
The genera Aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. These are
cosmopolitan species, natural contaminants of agricultural products. In coffee grains, the most important Aspergillus species in
terms of the risk of presenting mycotoxins belong to the genera Aspergillus Section Circumdati and Section Nigri. The purpose of
this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cultivation
from the South of Minas Gerais, Brazil, as well as to evaluate which farming system presents higher contamination risk by
ochratoxin A (OTA) produced by fungi. Thirty samples of coffee grains (Coffea arabica L.) were analysed, being 20 of them of...
Tipo: Journal article
Palavras-chave: Aspergillus ochraceus; Ochratoxin A; Arabica coffee.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200006
Identification of genes differentially expressed in hyphae of Candida albicans
Provedor de dados: 58
Autores: Melo,Analy Salles de Azevedo; Serafim,Rui Cosme; Briones,Marcelo Ribeiro da Silva.
The ability to switch from yeast to hyphal forms is essential for Candida albicans virulence. This morphological switch involves
the expression of hyphal-specific genes under the control of transcriptional factors. To contribute to the discovery of
hyphal-specific genes, we used a differential screening method where clones of a genomic DNA library were hybridized with
yeast and hyphal cDNA probes. Two clones with increased expression in hyphae were selected for study. Sequencing these
clones, we found that they encoded two important metabolic genes, CaHXT7 (high-affinity hexose transporter) and CaYLL34
(member of the AAA ATPase family). CaHXT7 and CaYLL34 ORFs were completely determined. Analyses of the putative
proteins show that: (1) CaHxt7p has one...
Tipo: Journal article
Palavras-chave: Differential screening; Candida albicans; HXT7; YLL34.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500046
Development of conventional and real-time multiplex PCR assays for the detection of nosocomial
pathogens
Provedor de dados: 58
Autores: Anbazhagan,Deepa; Mui,Wang Seok; Mansor,Marzida; Yan,Gracie Ong Siok; Yusof,Mohd Yasim; Sekaran,Shamala
Devi.
Nosocomial infections are major clinical threats to hospitalised patients and represent an important source of morbidity and
mortality. It is necessary to develop rapid detection assays of nosocomial pathogens for better prognosis and initiation of
antimicrobial therapy in patients. In this study, we present the development of molecular methods for the detection of six common
nosocomial pathogens including Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae,
Pseudomonas aeruginosa and Acinetobacter spp. Conventional multiplex PCR and SYBR Green based real time PCR assays were
performed using genus and species specific primers. Blind testing with 300 clinical samples was also carried out. The two assays
were found to be...
Tipo: Journal article
Palavras-chave: Conventional multiplex PCR; SYBR Green Real time PCR; Nosocomial Pathogens.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200006
Anti-Helicobacter pylori activity of plant extracts traditionally used for the treatment of
gastrointestinal disorders
Provedor de dados: 58
Autores: Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Miguel,Marilis Dallarmi; Miguel,Obdulio Gomes; Cunico,Miriam
Machado; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Kussen,Gislene Maria Botão; Nogueira,Keite da Silva;
Costa,Libera Maria Dalla.
The antibacterial activity of plant extracts obtained from Bixa orellana L., Chamomilla recutita L., Ilex paraguariensis A. St.-Hil.,
Malva sylvestris L., Plantago major L. and Rheum rhaponticum L. has been evaluated against two reference strains and eleven
clinical isolates of Helicobacter pylori. All the plant species chosen are used in popular Brazilian cuisine and folk medicine in the
treatment of gastrointestinal disorders. Initial screening was made by the disk diffusion test and then minimum inhibitory
concentration was determined by the agar dilution method. The results presented in this work demonstrated that among the plant
preparations analyzed, B. orellana L., C. recutita L., I. paraguariensis A. St.-Hil. and M. sylvestris L. were capable of...
Tipo: Journal article
Palavras-chave: Helicobacter pylori; Antibacterial activity; Plant extracts.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200007
Assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of Bacillus cereus
from a dairy plant
Provedor de dados: 58
Autores: Bernardes,Patrícia Campos; Andrade,Nélio José de; Ferreira,Sukarno Olavo; Sá,João Paulo Natalino de;
Araújo,Emiliane Andrade; Delatorre,Deyse Maria Zanom; Luiz,Lívia Maria Pinheiro.
The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial
interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or
absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel
adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied
was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically
favorable (ΔGadhesion > 0) between the stainless steel and the strain of B. cereus studied. Thus, the...
Tipo: Journal article
Palavras-chave: Hydrophobicity; Roughness; Bacillus cereus; Dairy plant; Adhesion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400017
In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus
Provedor de dados: 58
Autores: Pereira,Cássio Vicente; Pereira,Luciano José; Gonçalves,Reginaldo Bruno; Höfling,José Francisco.
The in vitro study of the interactions between S. mutans and S. sobrinus is important to determine the role of these
microorganisms in the formation of biofilms on dental structures and their potential to induce carious lesions. The objective of this
research was to study the suppression of bacterial plaque formation and its recolonization by rifampycin-resistant S.mutans and
streptomycin-resistant S. sobrinus. To study the competitive relationship between these species, previously standardized strains
were incubated in media containing different fermentable carbohydrates. At determined time intervals, samples were collected
from mixed cultures of S. mutans and S. sobrinus, diluted and plated on BHI-agar containing rifampycin or streptomycin to
determine...
Tipo: Journal article
Palavras-chave: Streptococcus mutans; Streptococcus sobrinus; Suppression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100004
Chemical modification of Aspergillus nigerβ-glucosidase and its catalytic properties
Provedor de dados: 58
Autores: Ahmed,Samia A.; El-Shayeb,Nefisa M.A.; Hashem,Abdel-Gawad M.; Saleh,Shireen A.A.; Abdel-Fattah,Ahmed F.
Aspergillus niger β-glucosidase was modified by covalent coupling to periodate activated polysaccharides
(glycosylation). The conjugated enzyme to activated starch showed the highest specific activity (128.5 U/mg protein). Compared
to the native enzyme, the conjugated form exhibited: a higher optimal reaction temperature, a lower Ea (activation energy), a
higher Km (Michaelis constant) and Vmax (maximal reaction rate), and improved thermal stability. The calculated t1/2 (half-life)
values of heat in-activation at 60 °C and 70 °C were 245.7 and 54.5 min respectively, whereas at these temperatures the native
enzyme was less stable (t1/2of 200.0 and 49.5 min respectively). The conjugated enzyme retained 32.3 and 29.7%, respectively
from its initial...
Tipo: Journal article
Palavras-chave: β -glucosidases glycosylation soluble polysaccharides enzyme stability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100023
Susceptibility of Nasutitermes ehrhardti (Isoptera: Termitidae) to Bacillus thuringiensis subspecies
Provedor de dados: 58
Autores: Castilhos-Fortes,Raquel de; Matsumura,Aida T.S.; Diehl,Elena; Fiuza,Lidia M.
The effects of Bacillus thuringiensis (Bt) Berliner on the termite Nasutitermes ehrhardti (Isoptera, Termitidae) were evaluated
under laboratory conditions. From 55 Bt subspecies assayed in vivo under controlled conditions seven were found to be
pathogenic in the subspecies yunnanensis, huazhongiensis, brasiliensis, colmeri and kurstaki (less than 72% of mortality),
particularly sooncheon and roskildiensis (100% mortality at the seventh day after the bacteria application). The LC50 for
subspecie sooncheon corresponded to 47x10(8),()66.2x10(6) and 5.1x10(5) cells/ml, at the third, fifth and seventh day,
respectively. For the subspecie roskildiensis the LC50 corresponded to 30.8x10(5), 48.4x10(6) and 16.8x10(4) cells/ml, at the
third, fifth and seventh day,...
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Biological control; Entomopathogenic bacteria; Nasutitermes ehrhardti; Termite.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300006
Use of single-enzyme amplified fragment length polymorphism for typing Clostridium perfringens
isolated from diarrheic piglets
Provedor de dados: 58
Autores: Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke.
Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic
enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present
study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from
swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial
correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples.
Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed...
Tipo: Journal article
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034
Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus
NCFT 4269.10
Provedor de dados: 58
Autores: Sethi,Bijay Kumar; Nanda,Prativa Kumari; Sahoo,Santilata.
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state
fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for
96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was
purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3 kDa as
determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of
50 °C, and substrate concentration of 1.5%. The enzyme was thermostable at 60 °C for 1 h, and the optimum
enzyme–substrate reaction time...
Tipo: Journal article
Palavras-chave: Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100143
Clinical significance, antimicrobial susceptibility and molecular identification of Nocardia species
isolated from children with cystic fibrosis
Provedor de dados: 58
Autores: Betrán,Ana; Villuendas,Mª Cruz; Rezusta,Antonio; Pereira,Javier; Revillo,Mª José; Rodríguez-Nava,Verónica.
ABSTRACT Nocardia is an opportunistic pathogen that causes respiratory infections in immunocompromised patients. The aim
of this study was to analyze the epidemiology, clinical significance and antimicrobial susceptibility of Nocardia species isolated
from eight children with cystic fibrosis. The isolated species were identified as Nocardia farcinica, Nocardia transvalensis,
Nocardia pneumoniae, Nocardia veterana and Nocardia wallacei. N. farcinica was isolated in three patients and all of them
presented lung affectation with a chronic colonization and pneumonia. N. farcinica showed resistance against gentamicin,
tobramycin, cefotaxime, but was susceptible to trimethoprim-sulfamethoxazole and amikacin. N. transvalensis, which was isolated
from two...
Tipo: Journal article
Palavras-chave: Nocardia; Cystic fibrosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300531
Some factors affecting tannase production by Aspergillus niger Van Tieghem
Provedor de dados: 58
Autores: Aboubakr,Hamada A.; El-Sahn,Malak A.; El-Banna,Amr A.
One variable at a time procedure was used to evaluate the effect of qualitative variables on the production of tannase from
Aspergillus niger Van Tieghem. These variables including: fermentation technique, agitation condition, tannins source, adding
carbohydrates incorporation with tannic acid, nitrogen source type and divalent cations. Submerged fermentation under
intermittent shaking gave the highest total tannase activity. Maximum extracellular tannase activity (305 units/ 50 mL) was
attained in medium containing tannic acid as tannins source and sodium nitrate as nitrogen source at 30 ºC for 96 h. All added
carbohydrates showed significant adverse effects on the production of tannase. All tested divalent cations significantly decreased
tannase...
Tipo: Journal article
Palavras-chave: Tannase; Aspergillus niger Van Tieghem; Factors affecting tannase production; Split plot design.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200036
The use of alpha-methyl-D-glucoside, a synthetic analogue of maltose, as inducer of amylase by
Aspergillus sp in solid-state and submerged fermentations
Provedor de dados: 58
Autores: Moreira,Fabiana G.; Lenartovicz,Veridiana; Souza,Cristina G.M. de; Ramos,Edivan P.; Peralta,Rosane M.
The use of a methyl-D-glucoside (alphaMG), a synthetic analogue of maltose, as carbon source and inducer of amylase synthesis
to several species of Aspergillus was studied in submerged and solid-state fermentations. Among a group of ten species, A.
tamarii, A. fumigatus and A. flavus were able to produce biomass and high specific amylolytic activity in submerged cultures
containing alphaMG as the only carbon source. In solid state fermentation, the enrichment of basal wheat bran or corn cob
medium with alphaMG increased up to 3 times the production of amylases. In both submerged and solid state fermentations,
alphaMG was more effective inducer of amylases than maltose and starch.
Tipo: Journal article
Palavras-chave: Amylase; Aspergillus; Solid-state fermentation; Submerged fermentation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100004
ANTAGONISTIC EFFECT OF EDIBLE MUSHROOM EXTRACT ON CANDIDA ALBICANS
GROWTH
Provedor de dados: 58
Autores: Paccola,Edneia A. de Souza; Maki,Cristina Sayuri; Nobrega,Gisele M.A. de; Paccola-Meirelles,Luzia Doretto.
Five species of edible mushrooms, Lentinula edodes, Pleurotus ostreatus, Pholiota nameko, Macrolepiota bonaerensis and
Agaricus blazei, were tested for their potential to inhibit the in vitro growth of the pathogenic yeast Candida albicans. Only L.
edodes had a fungistatic effect on this human pathogen. The inhibitory compound was produced intra and extracellularly in
submersed L. edodes culture, and was also present in fresh and dehydrated mushroom basidiocarps. The fungistatic compound
was heat sensitive and lost activity after 72 hours.
Tipo: Journal article
Palavras-chave: Mushrooms; Antibiotics; Candidiasis; Fungistatic effect.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300003
Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome
from Chinese longsnout catfish (Leiocassis longirostris Günther)
Provedor de dados: 58
Autores: Cai,Shuang-Hu; Wu,Zao-He; Jian,Ji-Chang; Lu,Yi-Shan; Tang,Ju-Feng.
273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains
conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA,
gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv.
veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion
method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested.
Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence
of strain...
Tipo: Journal article
Palavras-chave: Aeromonas veronii bv. veronii; Ulcerative syndrome; Leiocassis longirostris Günther; Identification.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100046
Isolation of bacteriocin-producing lactic acid bacteria from meat and meat products and its spectrum
of inhibitory activity
Provedor de dados: 58
Autores: Bromberg,Renata; Moreno,Izildinha; Zaganini,Cíntia Lopes; Delboni,Roberta Regina; Oliveira,Josiane de.
A total of 285 samples of meat and meat products were evaluated for the presence of bacteriocin-producing lactic acid bacteria by
the "sandwich" test. From 174 of these samples, 813 strains of lactic acid bacteria were isolated. They were able to inhibit the
growth of Staphylococcus aureus CTC 33 and/or Listeria innocua Lin 11. When evaluated by the well-diffusion assay, 128 of
these strains inhibited the growth of the indicator strains. The inhibitory spectra of activity of the isolates were evaluated against a
range of Gram-positive and Gram-negative test organisms. S. aureus was the most sensitive indicator tested, whereas
Enterococcus faecalis and Lactobacillus plantarum were the most resistant ones. All the compounds produced by the lactic acid...
Tipo: Journal article
Palavras-chave: Bacteriocins; Lactic acid bacteria; Meat; Inhibitory activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100023
Bacteriocin like substance production by Carnobacterium piscicola in a continuous system with three
culture broths. Study of antagonism against Listeria monocytogenes on vacuum packaged salmon
Provedor de dados: 58
Autores: Schöbitz,Renate P.; Bórquez,Pamela A.; Costa,Marcia E.; Ciampi,Luigi R.; Brito,Carmen S.
Three culture media were studied for the bacteriocin like substance (BLS) production from Carnobacterium piscicola L 103 in a
batch and continuous culture system. The efficacy of the antagonistic substance against Listeria monocytogenes was tested on
vacuum packaged salmon. BLS was produced in a 1.0 L bioreactor by batch and continuous culture using D-MRS, mod. D-MRS
and APT as nutrient broths. Salmon fillets were inoculated with BLS (200 AU mL-1 and 800 AU mL-1) and 8.0 x 10¹ cfu cm-2 of
L. monocytogenes and stored at 4ºC. The growth of L. monocytogenes was determined every 5 days during 15 days. After 12 h of
batch culture the stationary growth phase of C. piscicola L 103 started, with a BLS activity of 800 AU mL-1 in D-MRS and mod.
D-MRS broth, and of...
Tipo: Journal article
Palavras-chave: Carnobacterium piscicola; Bacteriocin like substance production; Continuous system; Listeria monocytogenes
antagonism; Vacuum packaged salmon.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100010
Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast
Saccharomyces cerevisiae using different carbon sources
Provedor de dados: 58
Autores: Pavezzi,Fabiana Carina; Gomes,Eleni; Silva,Roberto da.
Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for
production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast
Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized
physico-chemically. The enzyme presented high specific activity, 13.8 U/mgprotein or 2.9 U/mgbiomass, after 48 h of
fermentation using soluble starch as substrate. Glucoamylase presented optimum activity at temperature of 55ºC, and, in the
substratum absence, the thermostability was for 1h at 50ºC. The optimum pH of activity was pH 3.5 - 4.0 and the pH stability
between 5.0 and 7.0. The half life at...
Tipo: Journal article
Palavras-chave: Glucoamylase; Aspergillus awamori; Saccharomyces cerevisiae; Corn starch; Potato starch; Cassava starch.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100024
Interdigital foot infections: Corynebacterium minutissimum and agents of superficial mycoses
Provedor de dados: 58
Autores: Sariguzel,Fatma Mutlu; Nedret Koc,A.; Yagmur,Gülhan; Berk,Elife.
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused
by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the
prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot
infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of
20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In
identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic...
Tipo: Journal article
Palavras-chave: Interdigital foot infections; Superficial mycoses; Dermatophytosis; Yeast; Corynebacterium minutissimum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300003
Antimycobacterial activity of the antiinflammatory agent diclofenac sodium, and its synergism with
streptomycin
Provedor de dados: 58
Autores: Dutta,Noton K.; Dastidar,Sujata G.; Kumar,Asok; Mazumdar,Kaushiki; Ray,Raja; Chakrabarty,Atindra N.
Diclofenac sodium, an antiinflammatory agent, exhibited remarkable inhibitory action against both drug sensitive and drug
resistant clinical isolates of Mycobacterium tuberculosis, as well as other mycobacteria. This drug was tested in vitro against 45
different strains of mycobacteria, most of which were inhibited by the drug at 10-25 µg/ml concentration. When tested in vivo,
diclofenac, injected at 10 µg/g body weight of a Swiss strain of white mice, could significantly protect them when challenged with
50 median lethal dose of M. tuberculosis H37 Rv 102. According to chi2 test, the in vivo data were highly significant (p<0.01).
Diclofenac was further tested for synergism with the conventional antimycobacterial drug streptomycin against M. smegmatis...
Tipo: Journal article
Palavras-chave: Antiinflammatory drug; Diclofenac sodium; Antimycobacterial activity; Streptomycin; Synergism;
Non-antibiotic.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300009
Submerged fermentation of Lactobacillus rhamnosus YS9 for γ-aminobutyric acid
(GABA) production
Provedor de dados: 58
Autores: Lin,Qian.
γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter in central nervous system, and its application in
drugs and functional foods has attracted great attention. To enhance production of y-aminobutyric acid, Lactobacillus rhamnosus
YS9, a strain isolated from Chinese traditional fermented food pickled vegetable, was grown under submerged fermentation. Its
cultivation conditions were investigated. When culture pH condition was adjusted to the optimal pH of glutamate decarboxylase
activity, culture of Lb. rhamnosus YS9 in medium supplemented with 200 mM of monosodium glutamate and 200 µM of
pyridoxal phosphate (PLP), produced 187 mM of GABA.
Tipo: Journal article
Palavras-chave: Lactobacillus rhamnosus γ -aminobutyric acid (GABA) glutamate decarboxylase (GAD).
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100028
Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified
milk and of immobilized B. lactis in yoghurt
Provedor de dados: 58
Autores: Grosso,Carlos Raimundo Ferreira; Fávaro-Trindade,Carmen Sílvia.
This study evaluated the stability of Bifidobacterium lactis (Bb-12) and of Lactobacillus acidophilus (La-05) both free and
immobilized in calcium alginate, in milk and in acidified milk (pH 5.0, 4.4 and 3.8). The stability of immobilized B. lactis in
yoghurt (fermented to pH 4.2), during 28 days of refrigerated storage was also evaluated. The efficiency of two culture media
(modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue) for counting of B. lactis in yoghurt was determined.
Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were
counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of
survival in milk...
Tipo: Journal article
Palavras-chave: Probiotics; Immobilization; Alginate; Lactobacillus acidophilus; Bifidobacterium lactis; Yoghurt; Milk;
Lactobacillus bulgaricus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100025
New PHA products using unrelated carbon sources
Provedor de dados: 58
Autores: Matias,Fernanda; Rodrigues,Maria Filomena de Andrade.
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its
chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human
purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these
bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In
this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type,
Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The
classification was based on 16S...
Tipo: Journal article
Palavras-chave: Burkholderia; Burkholderia cepacia complex; Genomovar-type; Mcl-PHA; Polyhydroxyalkanoates;
Polyhydroxybutyrate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017
Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract
Provedor de dados: 58
Autores: Silva,S.Q.; Silva,D.C.; Lanna,M.C.S.; Baeta,B.E.L.; Aquino,S.F.
The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench
scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct
operational phases, when the reactor were stable (low variation of color removal), to assess the effect of glucose and yeast extract
as source of carbon and redox mediators, respectively. Reactors performance was evaluated based on COD (chemical oxygen
demand) and color removal. The microbial dynamics were investigated by PCR-DGGE (Polimerase Chain Reaction - Denaturing
Gradient of Gel Electrophoresis) technique by comparing the 16S rDNA profiles among samples. The results suggest that the
composition of...
Tipo: Journal article
Palavras-chave: Azo dye; UASB reactor; PCR-DGGE; Wastewater treatment; Microbial profile.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400005
Periodontal profile and presence of periodontal pathogens in young African-Americans from
Salvador, BA, Brazil
Provedor de dados: 58
Autores: Victor,Ligia Valéria; Cortelli,Sheila Cavalca; Aquino,Davi Romeiro; Carvalho Filho,Jonas de; Cortelli,José Roberto.
This cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic
subjects who live in Salvador/Bahia-Brazil and have never smoked. Periodontal Probing Depth (PPD), Clinical Attachment Level
(CAL), Plaque Index (PI) and Gingival Index (GI) were measured and analyzed by ANOVA and Wilcoxon tests (p<0.05)
according to gender and age. The presence of A.actinomycetemcomitans, P.gingivalis, E.corrodens and F.nucleatum was
determined by PCR and was analyzed by ANOVA, Wilcoxon, Student-t tests (p<0.05). Mean values of PPD and CAL were
2.18 and 1.0mm, respectively. Clinical parameters did not show differences between subjects of varying gender and age. The
microbial prevalence was observed to be...
Tipo: Journal article
Palavras-chave: African-Americans; Bacteria; Epidemiologic studies; Teen health; Periodontics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200005
Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize
Provedor de dados: 58
Autores: Aquino,Simone; Ferreira,Fabiane; Ribeiro,Deise Helena Baggio; Corrêa,Benedito; Greiner,Ralf; Villavicencio,Anna
Lucia Casañas Haasis.
One of the currently most important fungi in stored grains is Aspergillus flavus, which produce aflatoxins. This fungus can grow
on diverse substrates and represents a serious public health and animal nutritional problem. Therefore, the study of techniques that
can be applied to the control of aflatoxins is of great importance. The objective of the present study was to determine the effects of
gamma radiation on the growth of Aspergillus flavus Link and on degradation of aflatoxin B1 and B2 (AFB1 and AFB2) at a
relative humidity of 97 99% and a water activity (Aw) of 0.88-0.94. Samples of corn grains were irradiated using a cobalt 60
source emitting gamma rays at doses of 2, 5 and 10 kGy. Irradiation was found to be effective in reducing the number...
Tipo: Journal article
Palavras-chave: Gamma radiation; Aspergillus flavus; Fluorescent viability test; Water activity; Maize.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400009
Investigation of the association between clinical outcome and the cag pathogenicity-island and other
virulence genes of Helicobacter pylori isolates from patients with dyspepsia in Eastern Turkey
Provedor de dados: 58
Autores: Ozbey,Gokben; Demirel,Ulvi; Aygun,Cem; Ertas,Hasan Basri.
The aims of our work were to determine the presence of the cag pathogenicity-island (cag PAI) and other virulence genes of
Helicobacter pylori recovered from patients with gastritis and peptic ulcer, and to investigate the correlation of these virulence
genes with clinical outcome. The presence of the cagA, the promoter regions of cagA, cagE, cagT, and the left end of cag-PAI
(LEC), cag right junction (cagRJ), the plasticity region open reading frames (ORFs), vacA and oipA genes among 69 H. pylori
isolates were determined by polymerase chain reaction. Intact cag PAI was detected in only one (1.4%) isolate. The cagA gene
was identified in 52.1% and 76.2% of isolates from patients with dyspepsia (gastritis and peptic ulcer), respectively. The
plasticity...
Tipo: Journal article
Palavras-chave: Helicobacter pylori; Gastritis; Peptic ulcer; Cag pathogenicity-island; Polymerase chain reaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400034
Development of RFLP-PCR method for the identification of medically important Aspergillus species
using single restriction enzyme MwoI
Provedor de dados: 58
Autores: Diba,K.; Mirhendi,H.; Kordbacheh,P.; Rezaie,S.
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically
important Aspergillus species. Our subjects included nine standard Aspergillus species and 205 Aspergillus isolates of approved
hospital acquired infections and hospital indoor sources. First of all, Aspergillus isolates were identified in the level of species by
using morphologic method. A twenty four hours culture was performed for each isolates to harvest Aspergillus mycelia and then
genomic DNA was extracted using Phenol-Chloroform method. PCR-RFLP using single restriction enzyme MwoI was performed
in ITS regions of rDNA gene. The electrophoresis data were analyzed and compared with those of morphologic identifications.
Total of...
Tipo: Journal article
Palavras-chave: Aspergillus; Identification; MwoI.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200018
Isolation of dermatophytes in wild felids from screening centers
Provedor de dados: 58
Autores: Albano,Ana Paula N.; Nascente,Patrícia da Silva; Leite,Alice T. Meirelles; Xavier,Melissa O.; Santin,Rosema;
Mattei,Antonella Souza; Humberg,Roberta M.P.; Coimbra,Marco Antonio A.; Minello,Luiz Fernando; Meireles,Mario C.A.
The aim of this study was detect the presence of dermatophyte fungi on wild felids from screening centers. Samples were taken
from 30 animals, assembled in two groups: "free-ranging" and "transitory captivity". The dermatophytes (Trichophyton genus),
isolated from two felids (6.6%), both of the group "free-ranging".
Tipo: Journal article
Palavras-chave: Trichophyton sp.; Dermatophytes; Wild felids.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100025
Typing Candida albicans oral isolates from healthy Brazilian schoolchildren using multilocus enzyme
electrophoresis reveals two highly polymorphic taxa
Provedor de dados: 58
Autores: Boriollo,Marcelo Fabiano Gomes; Spolidorio,Denise Madalena Palomari; Barros,Letizia Monteiro; Bassi,Rodrigo
Carlos; Garcia,José Antonio Dias; Costa,Ana Maria Duarte Dias; Rosa,Edvaldo Antonio Ribeiro; Höfling,José Francisco.
The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic
areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of
schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies
(Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that
populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly...
Tipo: Journal article
Palavras-chave: Candida albicans; Healthy schoolchildren; Geographic region; MLEE; Cluster analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300023
Antimicrobial resistance profile of Enterococcus spp isolated from food in Southern Brazil
Provedor de dados: 58
Autores: Riboldi,Gustavo Pelicioli; Frazzon,Jeverson; d'Azevedo,Pedro Alves; Frazzon,Ana Paula Guedes.
Fifty-six Enterococcus spp. strains were isolated from foods in Southern Brazil, confirmed by PCR and classified as Enterococcus
faecalis (27), Enterococcus faecium (23) and Enterococcus spp(6). Antimicrobial susceptibility tests showed resistance
phenotypes to a range of antibiotics widely administrated in humans such as gentamycin, streptomycin, ampicillin and
vancomycin.
Tipo: Journal article
Palavras-chave: Enterococcus spp; Antibiotic resistant enterococci; Food.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100021
Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from
bovine subclinical mastitis
Provedor de dados: 58
Autores: Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa
Godoy; Souza,Viviane de.
Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial
persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers.
The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus
biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a
PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo.
These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and...
Tipo: Journal article
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017
Regulation of gene expression: Cryptic β-glucoside (bgl) operon of Escherichia coli as a
paradigm
Provedor de dados: 58
Autores: Harwani,Dharmesh.
Bacteria have evolved various mechanisms to extract utilizable substrates from available resources and consequently acquire
fitness advantage over competitors. One of the strategies is the exploitation of cryptic cellular functions encoded by genetic
systems that are silent under laboratory conditions, such as the bgl (β-glucoside) operon of E. coli. The bgl operon of
Escherichia coli, involved in the uptake and utilization of aromatic β-glucosides salicin and arbutin, is maintained in a
silent state in the wild type organism by the presence of structural elements in the regulatory region. This operon can be activated
by mutations that disrupt these negative elements. The fact that the silent bgl operon is retained without accumulating...
Tipo: Journal article
Palavras-chave: Cryptic; Bgl operon; Beta-gluosides; GASP.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400003
Optimization studies on production of a salt-tolerant protease from Pseudomonas aeruginosa strain
BC1 and its application on tannery saline wastewater treatment
Provedor de dados: 58
Autores: Sivaprakasam,Senthilkumar; Dhandapani,Balaji; Mahadevan,Surianarayanan.
Treatment and safe disposal of tannery saline wastewater, a primary effluent stream that is generated by soaking salt-laden hides
and skin is one of the major problems faced by the leather manufacturing industries. Conventional treatment methods like solar
evaporation ponds and land composting are not eco-friendly as they deteriorate the ground water quality. Though, this waste
stream is comprised of high concentration of dissolved proteins the presence of high salinity (1-6 % NaCl by wt) makes it
non-biodegradable. Enzymatic treatment is one of the positive alternatives for management of such kind of waste streams. A novel
salt-tolerant alkaline protease obtained from P.aeruginosa (isolated from tannery saline wastewater) was used for enzymatic...
Tipo: Journal article
Palavras-chave: COD; Enzymatic degradation; Leather Industry; Salt-tolerant; Waste treatment.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400038
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the
southwestern region of the State of São Paulo, Brazil
Provedor de dados: 58
Autores: Lemes-Marques,Eneida Gonçalves; Cruz,Cristina Durante; Destro,Maria Teresa.
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and
genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From
January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo
Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated
by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also
serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the
isolates...
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Serotypes; Antibiotic resistance; Genotypic characterization; Listeriosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
Provedor de dados: 58
Autores: Mesquita,Adriano Queiroz de; Rezende,Cintia Silva Minafra e; Mesquita,Albenones José de; Jardim,Eurione Antonio
Garcia da Veiga; Kipnis,Ana Paula Junqueira.
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among
different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single
Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens,
and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is
associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination
and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition.
Twenty percent...
Tipo: Journal article
Palavras-chave: TLR-4; Single nucleotide polymorphism; Mastitis; Milk quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034
Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization
and polymerase chain reaction detection
Provedor de dados: 58
Autores: Lima,Marcos Antonio Pereira de; Ferreira,Márcia Valéria Pitombeira; Barros,Marcos Aurélio Pessoa; Pardini,Maria
Inês de Moura Campos; Ferrasi,Adriana Camargo; Rabenhorst,Silvia Helena Barem.
Epstein-Barr virus (EBV) has been associated with 10% of gastric carcinomas. The aim of this study was to determine the
frequency of EBV in gastric carcinomas in Brazil assessed by in situ hybridization (ISH) and PCR, which would contribute to the
characterization of the clinical and pathological aspects of EBV-associated gastric carcinomas. One hundred and ninety-two
gastric carcinoma cases were collected at hospitals in two Brazilian states. Seventy-three out of 151 cases were PCR(+), while
11/160 cases were ISH(+). Nine out of eleven ISH(+) cases displayed a diffuse staining pattern and 2 out of 11 a focal pattern.
Both techniques showed that the EBV(+) cases were characterized by their association with males, older patients, lower gastric
region,...
Tipo: Journal article
Palavras-chave: Epstein-Barr virus; Gastric carcinoma; PCR; ISH.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100048
Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources
Provedor de dados: 58
Autores: Furumura,Márcia T.; Figueiredo,Patricia M.S.; Carbonell,Gleize V.; Darini,Ana Lucia da Costa; Yano,Tomomasa.
Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced
hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was
detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03%
L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the
isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation
followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but
did not produce...
Tipo: Journal article
Palavras-chave: Enterococcus faecalis; Virulence factors; Hemolysin; Proteases; Lipase; Cytotoxin; Biofilm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300007
Bacteriocins from Lactobacillus plantarum production, genetic organization and mode of action:
produção, organização genética e modo de ação
Provedor de dados: 58
Autores: Todorov,Svetoslav D.
Bacteriocins are biologically active proteins or protein complexes that display a bactericidal mode of action towards usually
closely related species. Numerous strains of bacteriocin producing Lactobacillus plantarum have been isolated in the last two
decades from different ecological niches including meat, fish, fruits, vegetables, and milk and cereal products. Several of these
plantaricins have been characterized and the aminoacid sequence determined. Different aspects of the mode of action,
fermentation optimization and genetic organization of the bacteriocin operon have been studied. However, numerous of
bacteriocins produced by different Lactobacillus plantarum strains have not been fully characterized. In this article, a brief
overview of the...
Tipo: Journal article
Palavras-chave: Lactobacillus plantarum; Plantaricin; Mode of action; Genetic organization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200001
Selection of endophytic fungi from comfrey (Symphytum officinale L.) for in vitro biological control
of the phytopathogen Sclerotinia sclerotiorum (Lib.)
Provedor de dados: 58
Autores: Rocha,Rafaeli; Luz,Daniela Eleutério da; Engels,Cibelle; Pileggi,Sônia Alvim Veiga; Jaccoud Filho,David de Souza;
Matiello,Rodrigo Rodrigues; Pileggi,Marcos.
Biological control consists of using one organism to attack another that may cause economic damage to crops. Integrated Pest
Management (IPM) is a very common strategy. The white mold produced by Sclerotiniasclerotiorum (Lib.) causes considerable
damage to bean crops. This fungus is a soil inhabitant, the symptoms of which are characterized by water-soaked lesions covered
by a white cottony fungal growth on the soil surface and/or the host plant. Possible biological control agents taken from plants are
being investigated as phytopathogen inhibitors. These are endophytic microorganisms that inhabit the intercellular spaces of
vegetal tissues and are often responsible for antimicrobial production. The objective of the present study was to select
endophytic...
Tipo: Journal article
Palavras-chave: Biological control; Sclerotinia sclerotiorum (Lib.); Endophytic strain; Symphytum officinale L.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100011
Plant growth promoting capability and genetic diversity of bacteria isolated from mud volcano and
lime cave of Andaman and Nicobar Islands
Provedor de dados: 58
Autores: Venkadesaperumal,Gopu; Amaresan,Natrajan; Kumar,Krishna.
Twenty four bacterial strains from four different regions of mud volcano and lime cave were isolated to estimate their diversity,
plant growth promoting and biocontrol activities to use them as inoculant strains in the fields. An excellent antagonistic effect
against four plant pathogens and plant growth promoting properties such as IAA production, HCN production, phosphate
solubilization, siderophore production, starch hydrolysis and hydrolytic enzymes syntheses were identified in OM5 (Pantoea
agglomerans) and EM9 (Exiguobacterium sp.) of 24 studied isolates. Seeds (Chili and tomato) inoculation with plant growth
promoting strains resulted in increased percentage of seedling emergence, root length and plant weight. Results indicated that
co-inoculation...
Tipo: Journal article
Palavras-chave: Plant growth promotion; Phytopathogens; Mud volcano; Lime cave; Amplified ribosomal DNA restriction
analysis (ADRDA).
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400018
Evaluation of pasture soil productivity in the semi-arid zone of Brazil by microbial analyses
Provedor de dados: 58
Autores: Luna,Rômulo Gil de; Coutinho,Henrique Douglas Melo; Grisi,Breno Machado.
The productivity of a pasture soil (caatinga) located in the region of São João do Cariri, PB, Brazil was evaluated based an the
following microbiological parameters: biomass (measured by fumigation-incubation method), activity (estimated from basal
respiration and cellulose decomposition rate), qCO2, and Cmic : Corg ratio. This analysis demonstrated that livestock
management in the 'caatinga' is probably causing environment damage by affecting the soil properties, reducing the microbial
biomass and soil respiration and increasing the qCO2, affecting the recovery of this ecosystem.
Tipo: Journal article
Palavras-chave: Semi-arid; Biomass; Microbial activity; Pasture; Caatinga.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300001
The sensitivity of bacterial foodborne pathogens to Croton blanchetianus Baill essential oil
Provedor de dados: 58
Autores: Melo,Geiseanny Fernandes do Amarante; Costa,Ana Caroliny Vieira da; Garino Junior,Felício; Medeiros,Rosália
Severo; Madruga,Marta Suely; Queiroga Neto,Vicente.
The aim of this study was to assess the activity of essential oil extracted from the leaves of C. blanchetianus Baill, popularly
known as "marmeleiro", in inhibiting the growth and survival of pathogenic microorganisms in food by determining their survival
in vitro and by observing the behaviour of Listeria monocytogenes inoculated into a food model (meat cubes) that was stored at
refrigeration temperature (7 ± 1 ºC) for 4 days. The results indicated a bactericidal effect against Aeromonas hydrophila and
Listeria monocytogenes and bacteriostatic action against Salmonella Enteritidis. A bacteriostatic effect on meat contaminated with
L. monocytogenes was found for all concentrations of essential oils tested. These results showed that essential oil from the...
Tipo: Journal article
Palavras-chave: Essential oil; Croton blanchetianus Baill; Antimicrobial activity; Foodborne microorganisms.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400023
Detection of Cryptosporidium spp. Oocysts in raw sewage and creek water in the city of São Paulo,
Brazil
Provedor de dados: 58
Autores: Farias,Eveline Wilma Coutinho; Gamba,Rosa C.; Pellizari,Vivian Helena.
The protozoan parasite Cryptosporidium has emerged as one of the most important contaminants of water, causing waterborne
outbreaks of gastroenteritis worldwide. To monitor and understand the public health significance of this pathogen in
environmental samples, several methods have been developed to isolate and detect Cryptosporidium oocysts. The purpose of this
study was to perform the first investigation on the presence of Cryptosporidium spp. oocysts in raw sewage and creek water in the
city of São Paulo, Brazil. The oocysts were concentrated by flocculation and membrane filtration. The results showed the
occurrence of Cryptosporidium spp. in all wastewater samples analyzed, indicating a possible risk for dissemination of these
pathogens in aquatic...
Tipo: Journal article
Palavras-chave: Cryptosporidium spp.; Sewage; Creek waters.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100008
Preparation, characterization and in vitro antimicrobial activity of liposomal ceftazidime and
cefepime against Pseudomonas aeruginosa strains
Provedor de dados: 58
Autores: Torres,Ieda Maria Sapateiro; Bento,Etiene Barbosa; Almeida,Larissa da Cunha; Sá,Luisa Zaiden Carvalho Martins de;
Lima,Eliana Martins.
Pseudomonas aeruginosa is an opportunistic microorganism with the ability to respond to a wide variety of environmental
changes, exhibiting a high intrinsic resistance to a number of antimicrobial agents. This low susceptibility to antimicrobial
substances is primarily due to the low permeability of its outer membrane, efflux mechanisms and the synthesis of enzymes that
promote the degradation of these drugs. Cephalosporins, particularty ceftazidime and cefepime are effective against P. aeruginosa,
however, its increasing resistance has limited the usage of these antibiotics. Encapsulating antimicrobial drugs into unilamellar
liposomes is an approach that has been investigated in order to overcome microorganism resistance. In this study, antimicrobial...
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Liposomes; Cephalosporins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300020
Bioprospecting thermophiles for cellulase production: a review
Provedor de dados: 58
Autores: Acharya,Somen; Chaudhary,Anita.
Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial
processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes.
Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol
production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic
biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the
physiology of thermophilic cellulases producers and process development for enzyme production and biomass...
Tipo: Journal article
Palavras-chave: Lignocelluloses; Bioethanol; Cellulase; Thermophiles.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300001
Obtaining partial purified xylose reductase from Candida guilliermondii
Provedor de dados: 58
Autores: Tomotani,Ester Junko; Arruda,Priscila Vaz de; Vitolo,Michele; Felipe,Maria das Graças de Almeida.
The enzymatic bioconversion of xylose into xylitol by xylose reductase (XR) is an alternative for chemical and microbiological
processes. The partial purified XR was obtained by using the following three procedures: an agarose column, a membrane reactor
or an Amicon Ultra-15 50K Centrifugal Filter device at yields of 40%, 7% and 67%, respectively.
Tipo: Journal article
Palavras-chave: Xylose reductase; Xylitol; Partial purification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300027
Experimental infection of laying hens with Salmonella enterica serovar Gallinarum
Provedor de dados: 58
Autores: Oliveira,Gláucia Helaine de; Berchieri Junior,Angelo; Fernandes,Alexandre César.
Experimental infections were set up in commercial laying birds, comprising a white relatively resistant line and a red susceptible
line infecting with Salmonella enterica serovar Gallinarum. The major findings were that in susceptible birds clinical disease
occurred in a dose-dependent manner. Faecal excretion occurred in susceptible birds almost up to death but also occurred in the
more resistant line and in birds, which were convalescing. Removal of birds, which had died from the disease, from the
environment, reduced the resultant mortality/morbidity and may be regarded as a useful measure for control.
Tipo: Journal article
Palavras-chave: Laying hens; Salmonella Gallinarum; Contact infection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100011
Soil amendment with sewage sludge and its impact on soil microflora
Provedor de dados: 58
Autores: Vieira,Rosana Faria; Silva,Célia Maria Maganhotto de Souza.
The objective of this work was to verify the effect of frequent sludge soil amendments on the soil's activity and microbial biomass
C. Results showed that the use of excessive sludge doses could be detrimental to the soil's microflora.
Tipo: Journal article
Palavras-chave: Microorganisms; Nitrogen; Biosolid.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500019
Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender
and experimentally inoculated with Leptospira santarosai serovar guaricura
Provedor de dados: 58
Autores: Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna;
Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda.
Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated.
Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without
antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000
µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into
the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The
antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive...
Tipo: Journal article
Palavras-chave: Leptospires; Semen; Antibiotics; Extender.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011
Avaliação da biodegradação de parafinas e da produção de biosurfactante por Bacillus subtilis na
presença de petróleo
Provedor de dados: 58
Autores: Queiroga,Carmen Lucia; Nascimento,Lídia Regina; Serra,Gil Eduardo.
Os experimentos com Bacillus subtilis para avaliação da tensão superficial foram realizados com meio de cultivo contendo como
nutrientes básicos 0,4% de ions nitrato e 4% de glicose, na presença de petróleo. A produção de surfactina foi observada pela
redução da tensão superficial do meio de cultura fermentado. Surfactina foi isolada a partir do meio de cultura fermentado por B.
subtilis, por precipitação ácida seguida de extração com clorofórmio-metanol. A avaliação da composição dos alcanos lineares
(parafinas) foi realizada por cromatografia gasosa. Observamos uma significativa redução da tensão superficial do meio de cultura
indicando que a produção de biosurfactante não foi inibida pela presença de parafina, e que as parafinas leves podem ter sido...
Tipo: Journal article
Palavras-chave: Bacillus subtilis; Surfactina; Tensão superficial; Parafinas; Petróleo; Cromatografia gasosa.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400006
Detection of quinolone-resistance mutations in Salmonella spp. strains of epidemic and poultry origin
Provedor de dados: 58
Autores: Souza,Roberta Barreiros de; Magnani,Marciane; Ferrari,Rafaela Gomes; Kottwitz,Luciana Bill Mikito; Sartori,Daniele;
Tognim,Maria Cristina Bronharo; Oliveira,Tereza Cristina R. M. de.
Mutations into codons Aspartate-87 (62%) and Serine-83 (38%) in QRDR of gyrA were identified in 105 Salmonella strains
resistant to nalidixic acid (94 epidemic and 11 of poultry origin). The results show a high incidence of mutations associated to
quinolone resistance but suggest association with others mechanisms of resistance.
Tipo: Journal article
Palavras-chave: Quinolones; GyrA; Quinolone Resistance Determining Region (QRDR); AS-PCR-RFLP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100026
Applications of recombinant Pichia pastoris in the healthcare industry
Provedor de dados: 58
Autores: Weinacker,Daniel; Rabert,Claudia; Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.
Since the 1970s, the establishment and development of the biotech industry has improved exponentially, allowing the commercial
production of biopharmaceutical proteins. Nowadays, new recombinant protein production is considered a multibillion-dollar
market, in which about 25% of commercial pharmaceuticals are biopharmaceuticals. But to achieve a competitive production
process is not an easy task. Any production process has to be highly productive, efficient and economic. Despite that the perfect
host is still not discovered, several research groups have chosen Pichia pastoris as expression system for the production of their
protein because of its many features. The attempt of this review is to embrace several research lines that have adopted Pichia...
Tipo: Journal article
Palavras-chave: Yeast; Heterologous expression system; Recombinant protein; Production; Scale up.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400004
Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice
Provedor de dados: 58
Autores: Gaetti-Jardim Júnior,Elerson; Luvizotto,Maria C. Rui; Avila-Campos,Mario J.
The lethal action in Balb/c mice of 80 oral Fusobacterium nucleatum recovered from patients with adult periodontitis, healthy
subjects or Cebus apella monkeys was studied. Mice were inoculated intraperitoneally with each bacterial inoculum of 5 x 10(8)
CFU/ml. All the clinical isolates induced weight and coordinated movements loss. Pathological alterations in liver, CNS, heart,
and kidney with inflammatory reactions or vascular congestion were observed. Of all the tested F. nucleatum isolates, 61.2% from
periodontal patients, 57.1% from healthy subjects and 60% from monkeys, were capable of killing the mice in 48h. The clinical
isolates were significantly more pathogenic than F. nucleatum ATCC 10953 or ATCC 25586. B. fragilis ATCC 23745 showed
lethality...
Tipo: Journal article
Palavras-chave: Fusobacterium nucleatum; Pathogenesis; Human and non-human primates.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200016
Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil
Provedor de dados: 58
Autores: Silva,Lidiane Roberta Cruz da; Souza,Odacy Camilo de; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa;
Coelho,Rosalie Reed Rodrigues; Souza-Motta,Cristina Maria.
Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to
microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp,
but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at
two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases
and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three
most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the...
Tipo: Journal article
Palavras-chave: Shrimp; Fungi; Water; Protease; Aflatoxin B.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100007
A new approach to microbial production of gallic acid
Provedor de dados: 58
Autores: Bajpai,Bhakti; Patil,Shridhar.
In a new approach to microbial gallic acid production by Aspergillus fischeri MTCC 150, 40gL-1 of tannic acid was added in two
installments during the bioconversion phase of the process (25gL-1 and 15gL-1 at 32 and 44h respectively). The optimum
parameters for the bioconversion phase were found to be temperature: 35ºC, pH: slightly acidic (3.3-3.5), aeration: nil and
agitation: 250 rpm. A maximum of 71.4% conversion was obtained after 71h fermentation with 83.3% product recovery. The
yield was 7.35 g of gallic acid per g of biomass accumulated and the fermenter productivity was 0.56 g of gallic acid produced per
liter of medium per hour.
Tipo: Journal article
Palavras-chave: Tannase; Gallic acid; Tannic acid; Aspergillus fischeri.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400021
Identification and antimicrobial resistance of microflora colonizing feral pig (Sus scrofa) of Brazilian
Pantanal
Provedor de dados: 58
Autores: Lessa,SS; Paes,RCS; Santoro,PN; Mauro,RA; Vieira-da-Motta,O.
Antimicrobial resistance of bacteria is a worldwide problem affecting wild life by living with resistant bacteria in the
environment. This study presents a discussion of outside factors environment on microflora of feral pigs (Sus scrofa) from
Brazilian Pantanal. Animals had samples collected from six different body sites coming from two separated geographic areas,
Nhecolandia and Rio Negro regions. With routine biochemical tests and commercial kits 516 bacteria were identified, with 240
Gram-positive, predominantly staphylococci (36) and enterococci (186) strains. Among Gram-negative (GN) bacteria the
predominant specimens of Enterobacteriaceae (247) mainly represented by Serratia spp. (105), Escherichia coli (50), and
Enterobacter spp. (40) and specimens...
Tipo: Journal article
Palavras-chave: Brazilian Pantanal; Feral pig; Antibiotic resistance pattern.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200042
Tannase production by Penicillium atramentosum KM under SSF and its applications in wine
clarification and tea cream solubilization
Provedor de dados: 58
Autores: Selwal,Manjit K; Yadav,Anita; Selwal,Krishan K; Aggarwal,N.K; Gupta,Ranjan; Gautam,S. K.
Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so
there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as
Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues
like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar
(Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds
was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was
found...
Tipo: Journal article
Palavras-chave: Tannin acyl hydrolase; Agro residues; Penicillium atramentosum KM; Jamun leaves; SSF.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100047
Effect of the trehalose levels on the screening of yeast as probiotic by in vivo and in vitro assays
Provedor de dados: 58
Autores: Martins,Flaviano S.; Miranda,Ieso C.; Rosa,Carlos A.; Nicoli,Jacques R.; Neves,Maria J.
Probiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of the host when
ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed by in vitro assays simulating
gastrointestinal environment to determine the ability to survive in the digestive tract. In the present study, the possibility of
extrapolation of data obtained in in vitro assays to in vivo conditions was studied using five Saccharomyces cerevisiae strains
isolated from Brazilian Atlantic rain forest. Trehalose contents and survival after exposure to a combination of physiological
stresses generally found in the gastrointestinal tract of humans were determined for the five yeasts and compared to the behavior...
Tipo: Journal article
Palavras-chave: Probiotic; Saccharomyces boulardii; Saccharomyces cerevisiae; Stress; Trehalose.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100012
The iron uptake mechanisms of enteroinvasive Escherichia coli
Provedor de dados: 58
Autores: Andrade,Agda; Dall’ Agnol,Monique; Newton,Salete; Martinez,Marina B.
Enteroinvasive Escherichia coli strains (EIEC) of different serotypes isolated from patients with acute diarrhea were examined for
the ability to produce siderophores and iron-regulated outer membrane proteins (IROMP). For iron starvation cultures were grown
at 37°C in LB supplied with 200 muM of <FONT FACE="Symbol">a</font>-alpha’dypirydil. All strains
produced enterobactin and twelve (40%) produced aerobactin. The strains showed IROMP varying from 67-82 kDa. Proteins were
either induced or stimulated by the iron starvation. Differences were observed in the electrophoretic profile among the serotypes,
originating 5 electrophoretic profiles. All serotypes expressed proteins of 82 kDa (FepA) and 76 kDa (IutA) (except serotype
O28ac:H-...
Tipo: Journal article
Palavras-chave: Enteroinvasive E. coli; Iron uptake.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300009
High incidence of co-infection with Malaria and Typhoid in febrile HIV infected and AIDS patients in
Ekpoma, Edo State, Nigeria
Provedor de dados: 58
Autores: Agwu,E.; Ihongbe,J.C.; Okogun,G.R.A.; Inyang,N.J.
This survey was designed to determine the prevalence of Plasmodium falciparum and Salmonella Typhi among febrile HIV/AIDS
patients in Ekpoma. Malaria and typhoid risk factors in Ekpoma included occupation, poor health facilities and poor sanitation.
Malaria and typhoid are highly prevalent among Ekpoma HIV/AIDS patients.
Tipo: Journal article
Palavras-chave: Malaria; Typhoid; HIV/AIDS; Ekpoma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200022
Enteroxigenic Staphylococcus spp. and other microbial contaminants during production of Canastra
cheese, Brazil
Provedor de dados: 58
Autores: Borelli,Beatriz M.; Ferreira,Elaine G.; Lacerda,Inayara C.A.; Santos,Deise A.; Carmo,Luiz S.; Dias,Ricardo S.;
Silva,Maria Crisolita C.; Rosa,Carlos A.
Canastra cheese is produced from raw cow's milk, and it is made at the farmhouse level using artisanal procedures and natural
starters. The aim of this work was to determine the main hygienic-sanitary indicators and enterotoxigenic staphylococcal strains
present during the manufacturing of traditional cheese of Serra da Canastra region, Minas Gerais state, Brazil. Samples from 10
farms were studied, and they included: water employed in the process, raw milk, natural starters, cheese curd before salting and
cheese after five days of ripening. All water samples exhibited faecal coliform contamination above the maximum acceptable
value recommended by Brazilian standards. Pseudomonas aeruginosa and sulfite-reducing clostridia were also isolated from the
water...
Tipo: Journal article
Palavras-chave: Artisanal cheese; Coliforms; Staphylococcus; Enterotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400026
Ocurrence of Staphylococcus aureus and multiplex pcr detection of classic enterotoxin genes in cheese
and meat products
Provedor de dados: 58
Autores: Pelisser,Marcia Regina; Klein,Cátia Silene; Ascoli,Kelen Regina; Zotti,Thaís Regina; Arisi,Ana Carolina Maisonnave.
Multiplex PCR was used to investigate the presence of enterotoxins genes (sea, seb, sec, sed and see) and femA gene (specific for
Staphylococcus aureus) in coagulase-positive staphylococci (CPS) isolated from cheese and meat products. From 102 CPS
isolates, 91 were positive for femA, 10 for sea, 12 for sed and four for see.
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Multiplex PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100025
Conventional and alternative antifungal therapies to oral candidiasis
Provedor de dados: 58
Autores: Anibal,Paula Cristina; Sardi,Janaina de Cássia Orlandi; Peixoto,Iza Teixeira Alves; Moraes,Julianna Joanna de
Carvalho; Höfling,José Francisco.
Candida-associated denture stomatitis is the most common form of oral candidal infection, with Candida albicans being the
principal etiological agent. Candida adheres directly or via an intermediary layer of plaque-forming bacteria to denture acrylic.
Despite antifungal therapy to treat denture stomatitis, infection is reestablished soon after the treatment ceases. In addition, many
predisposing factors have been identified as important in the development of oral candidiasis, including malnourishment, common
endocrine disorders, such as diabetis mellitus, antibacterial drug therapy, corticosteroids, radiotherapy and other
immunocompromised conditions, such as acquired immunodeficiency syndrome (AIDS). These often results in increased
tolerance to the most...
Tipo: Journal article
Palavras-chave: Candida spp.; Antifungals; Medicinal plants; Extracts; Essential oil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400001
Successive cultivation of maize and agricultural practices on root colonization, number of spores and
species of arbuscular mycorrhizal fungi
Provedor de dados: 58
Autores: Carrenho,Rosilaine; Silva,Eraldo Schunk; Trufem,Sandra Farto Botelho; Bononi,Vera Lucia Ramos.
A large number of propagules and a broad spectrum of species are two important components of ecosystem (including
agroecosystem) sustainability. Previous studies carried out in temperate areas showed that repeated monoculture leads to a
decrease in the species abundance of arbuscular mycorrhizal fungi (AMF). This study evaluated the influence of maize
monoculture and its agricultural practices on AMF during three consecutive cropping years in a Brazilian field. At the end of each
cycle, soil and root samples were evaluated for species composition, spore populations and root colonization by AMF. The AMF
community present during this period was scored according to the Spearman rank correlation and Principal Components Analysis.
The mean percent root...
Tipo: Journal article
Palavras-chave: Monoculture; Maize; Mycorrhiza; Diversity of species.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400002
Bacteria of fecal origin in mangrove oysters (Crassostrea rhizophorae) in the Cocó River estuary,
Ceará State, Brazil
Provedor de dados: 58
Autores: Silva,Ana I.M.; Vieira,Regine H.S.F.; Menezes,Francisca G.R.; Fonteles-Filho,Antonio A.; Torres,Regina C.O.;
Sant'Anna,Ernani S.
This study was aimed at evaluating the microbiological quality of mangrove oysters (Crassostrea rhizophorae), collected at a
natural oyster bed in the estuary of Cocó river (Fortaleza, Ceará, Brazil). MPN values were used for estimating the total (TC) and
fecal (FC) coliforms and Enterococcus spp. TC and FC MPN values in the whole muscle and intervalve liquid ranged from
<1.8 to >1,600/g and from <1.8 to 920/g, respectively. The MPN estimates for Enterococcus spp. were between <3.0
and >1,100/g. No correlation was found between the physico-chemical parameters (temperature, salinity and pH) of the
surrounding water and the bacteriological contamination levels found in the tested oysters. The only correlation found was
between TC and FC...
Tipo: Journal article
Palavras-chave: Oyster; Total and fecal coliforms; Enterococci; Bacteriocins.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100021
Adherence and virulence genes of Escherichia colifrom children diarrhoea in the Brazilian Amazon
Provedor de dados: 58
Autores: Benevides-Matos,Najla; Pieri,Fabio A.; Penatti,Marilene; Orlandi,Patrícia P.
The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli. Studies of
epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing
countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in
children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes
and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho,
Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli. Out of 1,156 isolates obtained,...
Tipo: Journal article
Palavras-chave: Enteroadherent Escherichia coli; Diarrhoea; Children.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100131
Effectiveness of halo-tolerant, auxin producing Pseudomonas and Rhizobium strains to improve
osmotic stress tolerance in mung bean (Vigna radiata L.)
Provedor de dados: 58
Autores: Ahmad,Maqshoof; Zahir,Zahir A.; Nazli,Farheen; Akram,Fareeha; Arshad,Muhammad; Khalid,Muhammad.
Halo-tolerant, auxin producing bacteria could be used to induce salt tolerance in plants. A number of Rhizobium and auxin
producing rhizobacterial strains were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. The
selected strains were further screened for their ability to induce osmotic stress tolerance in mung bean seedlings under
salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of Rhizobium and Pseudomonas containing
ACC-deaminase were evaluated in combination, for their ability to induce osmotic stress tolerance in mung bean at original, 4,
and 6 dS m-1 under axenic conditions. Results showed that sole inoculation of Rhizobium and Pseudomonas strains improved the
total dry...
Tipo: Journal article
Palavras-chave: ACC-deaminase; Pseudomonas; Rhizobium; Osmotic stress; Salt tolerance index.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400045
Molecular characterization of a Xylanase-producing fungus isolated from fouled soil
Provedor de dados: 58
Autores: Sakthiselvan,Punniavan; Naveena,Balakrishnan; Partha,Nagarajan.
Xylanase (EC 3. 2. 1. 8), hydrolyzes xylo-oligosaccharides into D-xylose and required for complete hydrolysis of native cellulose
and biomass conversion. It has broad range of applications in the pulp and paper, pharmaceutical and Agri-food industries. Fifty
fungal species were isolated from the fouled soil around an oil refinery and screened for the production of xylanase enzyme by
enrichment culture techniques. The isolated fungal strain was identified as Hypocrea lixii SS1 based on the results of biochemical
tests and 18s rRNA sequencing. The phylogenetic tree was constructed using the MEGA 5 software. Further, Hypocrea lixii SS1
was tested for the ability to utilize the sunflower oil sludge (waste from the oil industry) as the sole carbon source for...
Tipo: Journal article
Palavras-chave: Fungal isolate; Growth kinetics; Hypocrea lixii SS1; Fermentation; Xylanase; Purification.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400020
Curli fimbria: an Escherichia coli adhesin associated with human cystitis
Provedor de dados: 58
Autores: Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out
by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs
(urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with
bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study
how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was
deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and
its...
Tipo: Journal article
Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414
Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus
ostreatus
Provedor de dados: 58
Autores: Machado,Kátia Maria Gomes; Matheus,Dácio Roberto.
Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have
been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of
bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate
colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were
provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization
was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish
peroxidase was used as...
Tipo: Journal article
Palavras-chave: Peroxidases; Laccase; Solid-state fermentation; "shimeji" culture residue.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400013
Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen
starvation
Provedor de dados: 58
Autores: Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira.
Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for
studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the
carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II
activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP
caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp
displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying
asparaginase II secretion under nitrogen...
Tipo: Journal article
Palavras-chave: Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent
protein.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
Purification and characterization of an extreme halothermophilic protease from a halophilic
bacterium Chromohalobacter sp. TVSP101
Provedor de dados: 58
Autores: Vidyasagar,Malashetty; Prakash,S.; Mahajan,Vineet; Shouche,Yogesh S.; Sreeramulu,K.
An extreme halophilic bacterium was isolated from solar saltern samples and identified based on biochemical tests and 16S r RNA
sequencing as Chromohalobacter sp. strain TVSP101. The halophilic protease was purified using ultrafiltration, ethanol
precipitation, hydrophobic interaction column chromatography and gel permeation chromatography to 180 fold with 22% yield.
The molecular mass of the protease determined by SDS PAGE was 66 kDa. The purified enzyme was salt dependent for its
activity and stability with an optimum of 4.5 M NaCl. The optimum temperature for maximum protease activity was 75°C. The
protease was optimally active at pH 8 and retained more than 80% of its activity in the range of pH 7-10. Sucrose and glycine at
10% (w/v) were the most...
Tipo: Journal article
Palavras-chave: Chromohalobacter sp. TVSP101; Halothermophilic protease; Purification; Organic solvents; Osmolytes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100002
Succession and growth strategy of a spring microbial community from kezhou sinter in China
Provedor de dados: 58
Autores: Yang,Hongmei; Lou,Kai.
The succession and growth strategy of a spring microbial community under earthquake action were investigated. The majority of
pre-earthquake isolates belonged to the Gammaproteobacteria, including two numerically dominant Stenotrophomonas sp. RB25
and Acinetobacter sp. RB11 (r-strategists). The predominant post-earthquake isolates were Alphaproteobacteria, with Rhizobium
sp. RA42 (K-strategists) being dominant among these organisms.
Tipo: Journal article
Palavras-chave: Growth strategy; Succession; Spring microbe; Earthquake.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100005
Screening of Trichoderma isolates for their potential of biosorption of nickel and cadmium
Provedor de dados: 58
Autores: Nongmaithem,Nabakishor; Roy,Ayon; Bhattacharya,Prateek Madhab.
Abstract Fourteen Trichoderma isolates were evaluated for their tolerance to two heavy metals, nickel and cadmium. Three
isolates, MT-4, UBT-18, and IBT-I, showed high levels of nickel tolerance, whereas MT-4, UBT-18, and IBT-II showed better
tolerance of cadmium than the other isolates. Under nickel stress, biomass production increased up to a Ni concentration of 60
ppm in all strains but then decreased as the concentrations of nickel were further increased. Among the nickel-tolerant isolates,
UBT-18 produced significantly higher biomass upon exposure to nickel (up to 150 ppm); however, the minimum concentration of
nickel required to inhibit 50% of growth (MIC50) was highest in IBT-I. Among the cadmium-tolerant isolates, IBT-II showed
both maximum biomass...
Tipo: Journal article
Palavras-chave: Heavy metals; Biosorption; Trichoderma; MIC50; Fungistasis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200305
Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter;
Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification
of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the
RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal
swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Tipo: Journal article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
Detection of multi drug resistant bacteria in major hospitals in Kano, North-West, Nigeria
Provedor de dados: 58
Autores: Yusuf,I.; Arzai,A.H.; Haruna,M.; Sharif,A.A.; Getso,M.I.
Two major hospitals in Kano, North West Nigeria have recorded increasing resistance of clinical pathogens to broad spectrum
β lactams, mediated by extended spectrum β- lactamase (ESβL) and non ESBLs. A study was
therefore undertaken to determine the occurrence and prevalence of plasmid and chromosomal mediated AmpC βL and
carbapenemase in addition to already known ESBL due to increasing resistance of pathogens from the two hospitals to
carbapenems, cephamycins and flouroquinolones. Antibiogram tests and ESBL, AmpC and carbapenemase production tests were
performed on all the isolates. AmpC and carbapenemase producers were further screened for AmpC inducibility and metallo beta
lactamase production respectively. Majority...
Tipo: Journal article
Palavras-chave: AmpC β -lactamases extended spectrum β -lactamases coexistence prevalence Gram
negative bacteria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300005
Comparison of thermotolerant coliforms and Escherichia coli densities in freshwater bodies
Provedor de dados: 58
Autores: Hachich,Elayse M.; Di Bari,Marisa; Christ,Ana Paula G.; Lamparelli,Cláudia C.; Ramos,Solange S.; Sato,Maria Inês Z.
Fecal bacterial indicator analyses have been widely used for monitoring the water quality. This study was designed to determine
the ratio between the density of Escherichia coli and other Thermotolerant Coliforms (TtC) bacteria from freshwater samples
collected for a two-year period of monitoring. TtC were enumerated by membrane filtration on mFC agar. E. coli enumeration
was done by two methods: TtC colonies identified in mFC were inoculated in EC-MUG or water samples were filtered and
inoculated in modified mTEC agar media, and both methods were compared for quantitative recovery of E. coli. The results
pointed out a mean percentage of E. coli among other thermotolerant coliforms (E. coli/TtC ratio) of 84.3% in mFC media. Taking
these results into...
Tipo: Journal article
Palavras-chave: Thermotolerant Coliforms (TtC); E. coli; Water quality; Microbiological standards.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200032
Yearly variation of bacterial production in the Arraial do Cabo protection area (Cabo Frio upwelling
region): an evidence of anthropogenic pressure
Provedor de dados: 58
Autores: Coelho-Souza,Sérgio A.; Pereira,Gilberto C.; Coutinho,Ricardo; Guimarães,Jean R.D.
Arraial do Cabo is where upwelling occurs more intensively on the Brazilian coast. Although it is a protection area it suffers
anthropogenic pressure such as harbor activities and sporadic sewage emissions. Short-time studies showed a high variability of
bacterial production (BP) in this region but none of them evaluated BP during long periods in a large spatial scale including
stations under different natural (upwelling and cold fronts) and anthropogenic pressures. During 2006, we sampled surface waters
10 times (5 in upwelling and 5 in subsidence periods) in 8 stations and we measured BP, temperature as well as the concentrations
of inorganic nutrients, pigments and particulate organic matter (POM). BP was up to 400 times higher when sewage emissions...
Tipo: Journal article
Palavras-chave: Bacterioplankton; Heterotrophic activity; Impacted area; Extractive reserve; Upwelling system.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400046
Characterization of Francisella species isolated from the cooling water of an air conditioning system
Provedor de dados: 58
Autores: Gu,Quan; Li,Xunde; Qu,Pinghua; Hou,Shuiping; Li,Juntao; Atwill,Edward R.; Chen,Shouyi.
Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains
are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition,
these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of
16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and
BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and
that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously...
Tipo: Journal article
Palavras-chave: Francisella; Air conditioning; Water.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300921
Agaricales Fungi from atlantic rain forest fragments in Minas Gerais, Brazil
Provedor de dados: 58
Autores: Rosa,Luiz Henrique; Capelari,Marina.
Two Atlantic Rain Forest fragments in Minas Gerais state were studied to access their Agaricales fungal richness. A total of 187
specimens were collected and 109 species, 39 genera, and eight families were identified. Thirty-three species were cited for the
first time in Brazil.
Tipo: Journal article
Palavras-chave: Basidiomycota; Biodiversity; Brazilian ecosystems; Threatened environment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400015
Assay of antioxidant potential of two Aspergillus isolates by different methods under various
physio-chemical conditions
Provedor de dados: 58
Autores: Arora,Daljit Singh; Chandra,Priyanka.
The objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot
blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp
(Aspergillus PR78 and Aspergillus PR66) showing quantitatively best antioxidant activity by DPPH assay were further tested for
their reducing power, ferrous ion and nitric oxide ion scavenging activity, FRAP assay and total phenolic content. Different
physio-chemical parameters were optimized for enhancement of the activity. This revealed stationary culture grown for 10 days at
25ºC at pH 7 to be the best for antioxidant activity. Sucrose in the medium as carbon source resulted in highest antioxidant...
Tipo: Journal article
Palavras-chave: Antioxidant potential; Aspergillus; Dot blot assay; DPPH assay; FRAP assay; Reducing power; Soil fungi.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300029
Antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives
Provedor de dados: 58
Autores: Garcia,Roxana; Alves,Eliomara S.S.; Santos,Mirella P.; Aquije,Glória M.F. Viégas; Fernandes,A. Alberto R.;
Santos,Reginaldo B. dos; Ventura,Jose A.; Fernandes,Patricia M.B.
Banana, papaya and pineapple are the most consumed tropical fruits in the world, being Brazil one of the main producers. Fungi
Colletotrichum musae, Colletotrichum gloeosporioides and Fusarium subglutinans f.sp. ananas cause severe post harvest diseases
and losses in fruits quality. The aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial
growth and conidia germination of these three phytopathogens. The monoterpenes citral, citronellal, L-carvone, isopullegol and
a-pinene were diluted in ethanol to final concentrations from 0.2 to 1%. All monoterpenes were found to inhibit the growth of the
three studies fungi in a dose-dependent manner. Citral was the most effective of the oils tested and showed potent fungicidal...
Tipo: Journal article
Palavras-chave: Biocontrol; Essential oils; Banana; Papaya; Pineapple; Plant diseases.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100032
Chitinase Production by Streptomyces sp. ANU 6277
Provedor de dados: 58
Autores: Narayana,Kolla J.P.; Vijayalakshmi,Muvva.
Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase
production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity
was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for
maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies
on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as
good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel...
Tipo: Journal article
Palavras-chave: Chitinase; Streptomyces sp. ANU 6277; Optimization; Characterization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400002
Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial
consortium
Provedor de dados: 58
Autores: Lalnunhlimi,Sylvine; Krishnaswamy,Veenagayathri.
Abstract Removal of synthetic dyes is one of the main challenges before releasing the wastes discharged by textile industries.
Biodegradation of azo dyes by alkaliphilic bacterial consortium is one of the environmental-friendly methods used for the removal
of dyes from textile effluents. Hence, this study presents isolation of a bacterial consortium from soil samples of saline
environment and its use for the decolorization of azo dyes, Direct Blue 151 (DB 151) and Direct Red 31 (DR 31). The
decolorization of azo dyes was studied at various concentrations (100–300 mg/L). The bacterial consortium, when
subjected to an application of 200 mg/L of the dyes, decolorized DB 151 and DR 31 by 97.57% and 95.25% respectively, within 5
days. The growth of...
Tipo: Journal article
Palavras-chave: Alkaliphilic bacterial consortium; Azo dye; Direct Blue 151; Direct Red 31; Decolorization; Salinity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100039
Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
Provedor de dados: 58
Autores: Matter,Letícia B.; Spricigo,Denis A.; Tasca,Caiane; de Vargas,Agueda C.
The invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal
Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian
pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a
zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this
study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was
conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli
SB278/94 from a...
Tipo: Journal article
Palavras-chave: GimB; Adherence; Invasiveness; Zoonotic potential; Livestock; Companion animals.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875
Use of the VITEK 2 system to identify and test the antifungal susceptibility of clinically relevant yeast
species
Provedor de dados: 58
Autores: Melhem,MSC; Bertoletti,A; Lucca,HRL; Silva,RBO; Meneghin,FA; Szeszs,MW.
Eleven quality control isolates (Candida albicans ATCC 64548, C. tropicalis ATCC 200956, C. glabrata ATCC 90030, C.
lusitaniae ATCC 200951, C. parapsilosis ATCC 22019, C. krusei ATCC 6258, C. dubliniensis ATCC 6330, Saccharomyces
cerevisiae ATCC 9763, Cryptococcus neoformans ATCC 90012, C. gattii FIOCRUZ-CPF 60, and Trichosporon mucoides ATCC
204094) and 32 bloodstream isolates, including C. albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, C. guilliermondii,
C. pelliculosa (Pichia anomala), C. haemulonii, C. lusitaniae, and C. kefyr were identified at the species level by the VITEK 2
system. A set of clinical isolates (32 total) were used as challenge strains to evaluate the ability of the VITEK 2 system to
determine the antifungal...
Tipo: Journal article
Palavras-chave: Antifungal agents; Candida; Susceptibility testing methods; MIC; CLSI; EUCAST.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400033
Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites
Provedor de dados: 58
Autores: Mercimek,Hatice Aysun; Dincer,Sadik; Guzeldag,Gulcihan; Ozsavli,Aysenur; Matyar,Fatih; Arkut,Afet; Kayis,Fikret;
Ozdenefe,Melis Sumengen.
Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using
Pseudomonas aeruginosa in in vitroexperiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg
L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples
taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid
chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and
59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium,
2,4-dinitrotoluene (2,4-DNT) and...
Tipo: Journal article
Palavras-chave: Pseudomonas sp; Biotransformation; Nitroaromatic explosives; TNT.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100103
Biotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery
through metagenomics
Provedor de dados: 58
Autores: Gomes,Elisângela Soares; Schuch,Viviane; Lemos,Eliana Gertrudes de Macedo.
The discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their
industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious
diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despite all of the
warnings about the failure of the "goose that laid the golden egg," the potential of this wealth is still inexorable: simply adjust the
focus from "micro" to "nano", that means changing the look from microorganisms to nanograms of DNA. Then, the search for
new drugs, driven by genetic engineering combined with metagenomic strategies, shows us a way to bypass the barriers imposed
by...
Tipo: Journal article
Palavras-chave: Environmental samples; Pharmacology; PKSs; New drugs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400002
Identification of Candida spp. by phenotypic tests and PCR
Provedor de dados: 58
Autores: Marinho,Sandra Aparecida; Teixeira,Alice Becker; Santos,Otávio Silveira; Cazanova,Ricardo Flores; Ferreira,Carlos
Alexandre Sanchez; Cherubini,Karen; Oliveira,Sílvia Dias de.
The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance,
allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from
oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans
from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture
in CHROMAgarTM Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic
confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germ-tubes, 73.7% produced
chlamydoconidia, and 63.2% showed...
Tipo: Journal article
Palavras-chave: Candida spp; Identification; PCR; Phenotypic tests.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200004
Application of microbial α-amylase in industry - A review
Provedor de dados: 58
Autores: Souza,Paula Monteiro de; Magalhães,Pérola de Oliveira e.
Amylases are one of the main enzymes used in industry. Such enzymes hydrolyze the starch molecules into polymers composed
of glucose units. Amylases have potential application in a wide number of industrial processes such as food, fermentation and
pharmaceutical industries. α-Amylases can be obtained from plants, animals and microorganisms. However, enzymes
from fungal and bacterial sources have dominated applications in industrial sectors. The production of α-amylase is
essential for conversion of starches into oligosaccharides. Starch is an important constituent of the human diet and is a major
storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. Starch-converting enzymes
are used in the...
Tipo: Journal article
Palavras-chave: α -Amylases enzyme production bacterial and fungal amylase starch.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400004
Tuberculosis determined by Mycobacterium bovis in captive waterbucks (Kobus ellipsiprymnus) in
São Paulo, Brazil
Provedor de dados: 58
Autores: Rocha,V.C.M.; Corrêa,S.H.R.; Oliveira,E.M.D.; Rodriguez,C.A.R.; Fedullo,J.D.; Matrone,M.; Setzer,A.; Ikuta,C.Y.;
Vejarano,M.P.; Figueiredo,S.M.; Ferreira Neto,J.S.
Two waterbucks from São Paulo Zoo Foundation exhibited respiratory symptoms in July 2004. After euthanasia, granulommas in
lungs and mediastinic lymph nodes were observed. Acid-fast bacilli isolated were identified as Mycobacterium bovis spoligotype
SB0121 by PRA and spoligotyping. They were born and kept in the same enclosure with the same group, without any contact to
other species housed in the zoo. This is the first detailed description of M. bovis infection in Kobus ellipsiprymnus.
Tipo: Journal article
Palavras-chave: Tuberculosis; Mycobacterium bovis; Kobus ellipsiprymnus; Waterbuck; Zoo.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200040
Vibrio cholerae O1 from superficial water of the Tucunduba Stream, Brazilian Amazon
Provedor de dados: 58
Autores: Sá,L.L.C.; Vale,E.R.V.; Garza,D.R.; Vicente,A.C.P.
Isolation and genetic characterization of an environmental Vibrio cholerae O1 from the Amazon is reported. This strain lacks two
major virulence factors - CTX and TCP - but carries other genes related to virulence. Genetic similarity with epidemic strains is
evaluated and the importance of V. cholerae surveillance in the Amazon is emphasized.
Tipo: Journal article
Palavras-chave: Vibrio cholerae O1; Environment; Amazon; Virulence genes; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200027
Identification of Mycobacterium bovis Isolates by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius;
Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for
RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR
successfully identified as M. bovis 88.24% of the isolates.
Tipo: Journal article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004
18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL
CHARACTERIZATION OF TRICHOPHYTON ISOLATES
Provedor de dados: 58
Autores: Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M.
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since
dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species
can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in
attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and
interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small
ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast
species,...
Tipo: Journal article
Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from
dialysis and kidney tranplant patients at a hosptial in northern Paraná
Provedor de dados: 58
Autores: Giarola,Luciana Borges; Santos,Rosiane Ribeiro dos; Tognim,Maria Cristina Bronharo; Borelli,Sueli Donizete;
Bedendo,João.
The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and
kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with
the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48
transplant patients, 75% were positive for S. aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded
conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both
were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion,...
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Nasal carrier; Resistance; MecA gene; Genetic typing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300011
Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora
neoaphidis
Provedor de dados: 58
Autores: Chen,Chun; Xie,Tingna; Ye,Sudan; Jensen,Annette Bruun; Eilenberg,J ørgen.
Abstract The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our
understanding of host–pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate
aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and
elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental
stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We
calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper
and Delta...
Tipo: Journal article
Palavras-chave: Pandora neoaphidis; Reference genes; Quantitative PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100259
Multiple drug resistance patterns in various phylogenetic groups of uropathogenic E.coli isolated
from Faisalabad region of Pakistan
Provedor de dados: 58
Autores: Bashir,Saira; Sarwar,Yasra; Ali,Aamir; Mohsin,Mashkoor; Saeed,Muhammad Azeem; Tariq,Ayesha; Haque,Abdul.
The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to
drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in
this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug
resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone,
ceftriaxone, cephradine among β-lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides;
nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E.
coli isolates...
Tipo: Journal article
Palavras-chave: Uropathogenic E. coli; Phylogenetic analysis; Drug resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400005
Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against
Crinipellis perniciosa, the causal agent of witches' broom of cocoa
Provedor de dados: 58
Autores: Marco,Janice Lisboa De; Valadares-Inglis,Maria Cléria; Felix,Carlos Roberto.
Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa,
were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the
absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, beta-1,3-glucanase, total cellulase, endoglucanase,
aryl- beta-glucosidase, beta-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence
of specific substrates. Except for N-acetylglucosaminidase and beta-glucosidase Trichoderma harzianum isolate 1051 produced
the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between
Trichoderma...
Tipo: Journal article
Palavras-chave: Trichoderma; Crinipellis perniciosa; Hydrolases; Antagonistic interaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100008
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
Provedor de dados: 58
Autores: Biberg,Camila Arguelo; Rodrigues,Ana Claudia Souza; Carmo,Sidiane Ferreira do; Chaves,Claudia Elizabeth Volpe;
Gales,Ana Cristina; Chang,Marilene Rodrigues.
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge
faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a
tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser
desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory
concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory
Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase
chain reaction (PCR), followed by...
Tipo: Journal article
Palavras-chave: Klebsiella pneumoniae; Carbapenems; Drug-resistant bacteria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501
Human T-Lymphotropic virus (HTLV) type I in vivo integration in oral keratinocytes
Provedor de dados: 58
Autores: Domínguez,Martha C; González,Norma Enith; Sánchez,Adalberto; García Vallejo,Felipe.
Although the infection of HTLV-1 to cell components of the mouth have been previously reported, there was not until this report,
a detailed study to show the characteristics of such infection. From 14 Tropical Spastic Paraparesis/ HTLV-1-Associated
Myelopathy (HAM/TSP) patients and 11 asymptomatic carrier individuals (AC) coming from HTLV-1 endemic areas of
southwest Pacific of Colombia, infected oral mucosa cells were primary cultured during five days. These cell cultures were
immunophenotyped by dual color fluorescence cell assortment using different lymphocyte CD markers and also were
immunohistochemically processed using a polyclonal anti-keratin antibody. Five days old primary cultures were characterized as
oral keratinocytes, whose phenotype was CD3-...
Tipo: Journal article
Palavras-chave: Oral keratinocytes; Oral mucosa; Human T-Lymphotropic virus type 1; Proviral integration; SIgA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100040
Novel agents for enzymatic and fungal hydrolysis of stevioside
Provedor de dados: 58
Autores: Milagre,H.M.S.; Martins,L.R.; Takahashi,J.A.
A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at
establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be
used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi
(Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae)andenzymes (pancreatin and
lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0,
using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8%
yield).
Tipo: Journal article
Palavras-chave: Stevioside; Hydrolysis; Aspergillus niger; Lipase; Pancreatin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200029
EFFECTS OF IMMOBILIZATION IN Ba-ALGINATE ON NITRILE-DEPENDENT OXYGEN
UPTAKE RATES OF CANDIDA GUILLIERMONDII
Provedor de dados: 58
Autores: Dias,João Carlos Teixeira; Rezende,Rachel Passos; Linardi,Valter Roberto.
Yeast cells immobilized by entrapment in Ba-alginate gel were investigated for growth pattern and respiratory activity. The
oxygen uptake rates (OUR) of cells entrapped in gels with 4% alginate were 5.2 and 23% lower than the OUR of 2% alginate and
free cells, respectively. The mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient
of nutrients throughout the gel which is responsible for both a lower specific growth rate of immobilized cells with respect to that
of free ones, and a heterogeneous biomass distribution, with progressively increasing cellular density from the inside to the
outside of the matrix. Gel-matrix polymer concentration affected the maximum oxygen uptake of immobilized growing yeast
cells.
Tipo: Journal article
Palavras-chave: Nitriles; Candida guilliermondii; Ba-alginate; Respiration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300011
Isolates of Ureplasma diversum genotyped by single-enzyme amplified length polymorphism
Provedor de dados: 58
Autores: Buzinhani,Melissa; Mettifogo,Elena; Buim,Marcos R.; Moreno,Andréa M.; Paixão,Renata; Timenetsky,Jorge.
Isolates of Ureaplasma diversum recovered from bovines with reproductive disorders and healthy ones of four premises were
compared by SE-AFLP. Twenty-eight SE-AFLP profiles without monomorphic fragments were obtained. The ureaplasma studied
were divided in clusters A and B. Cluster A was divided in subclusters A1 and A2, while A1 was divided in subclusters A1a and
A1b. Cluster B grouped only the reference strains. The clusters obtained were not associated with the reproductive disorders. The
dendrogram obtained showed high heterogeneity among the studied ureaplasmas and indicated a low genomic stability as detected
in other species of microorganisms of class Mollicutes.
Tipo: Journal article
Palavras-chave: Ureaplasma diversum; SE-AFLP; Bovine; Reproductive disorders.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100007
Evaluation of culture media for counts of Bifidobacterium animalis in the presence of yoghurt
bacteria
Provedor de dados: 58
Autores: Moriya,Juliana; Fachin,Luciano; Gândara,Ana Lourdes Neves; Viotto,Walkiria Hanada.
Much attention has been paid to the use of Bifidobacterium sp. in yoghurts due to their excellent therapeutic and nutritional
properties. However, in order to present health properties, it is recommended that each commercial product should indicate the
minimum daily amount required for it to confer health benefit(s) based on in vitro and human studies. Besides the inherent low
growth of Bifidobacterium sp in milk, there is a need for a reliable method for counting Bifidobacterium sp in the presence o f
yoghurt bacteria. This study evaluated the use of the media M-MRS, MRS-NNLP and RCPB pH5 aimed at counting the number
of Bifidobacterium animalis subesp. lactis in the presence of Streptococcus thermophilus and Lactobacillus delbrueckii subsp
bulgaricus after...
Tipo: Journal article
Palavras-chave: Count; Bifidobacterium; Lactic bacteria; Yoghurt.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400021
Production of Brazilian human norovirus VLPs and comparison of purification methods
Provedor de dados: 58
Autores: Lamounier,Thais Alves da Costa; Oliveira,Layssa Miranda de; Camargo,Brenda Rabello de; Rodrigues,Kelly Barreto;
Noronha,Eliane Ferreira; Ribeiro,Bergmann Morais; Nagata,Tatsuya.
Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for
the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of
vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the
Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and
ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Tipo: Journal article
Palavras-chave: Norwalk virus; VLP; Ion-exchange chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
Chemical and microbiological evaluation of ensiled sugar cane with different additives
Provedor de dados: 58
Autores: Bravo-Martins,Claudia Eugênia Castro; Carneiro,Heloisa; Castro-Gómez,Raúl Jorge Hernán; Figueiredo,Henrique
César Pereira; Schwan,Rosane Freitas.
Sugar cane silage has a potential for animal feeding, but uncontrolled growth of undesirable microorganisms may cause nutritional
losses and affect the animal productivity and health. The objective of this work was to evaluate the microbiological quality and
chemical composition of ensiled sugar cane with and without nutritional additives after 30 days of fermentation. Yeasts,
filamentous fungi and distinct groups of bacteria were enumerated by plate count methods and the chemical analyzes comprised
dry matter, crude protein, fiber content, lignin, and pH. Facultative aerobic bacteria and filamentous fungi were not detected
during the fermentative process in any of the treatments. The number of yeasts in five varieties of sugar cane silage without...
Tipo: Journal article
Palavras-chave: Sugar cane silage; Fermentation; Yeasts; Lactic acid bacteria.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400018
Microbicide activity of clove essential oil (Eugenia caryophyllata)
Provedor de dados: 58
Autores: Nuñez,L.; D' Aquino,M.
Clove essential oil, used as an antiseptic in oral infections, inhibits Gram-negative and Gram-positive bacteria as well as yeast.
The influence of clove essential oil concentration, temperature and organic matter, in the antimicrobial activity of clove essential
oil, was studied in this paper, through the determination of bacterial death kinetics. Escherichia coli, Staphylococcus aureus and
Pseudomonas aeruginosa were the microorganisms selected for a biological test. To determine the temperature effect, they were
assayed at 21° and 37° C. The concentration coefficient was determined with 0.4%, and 0.2% of essential oil. The influence of the
presence of organic matter was determined with 0.4% of essential oil. The results obtained demonstrated that...
Tipo: Journal article
Palavras-chave: Clove essential oil; Concentration coefficient; Organic matter; Antibacterial activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400003
Control of Colletotrichum gloeosporioides (penz.) Sacc. In yellow passion fruit using Cymbopogon
citratus essential oil
Provedor de dados: 58
Autores: Anaruma,Nina Duarte; Schmidt,Flávio Luís; Duarte,Marta Cristina Teixeira; Figueira,Glyn Mara; Delarmelina,Camila;
Benato,Eliane Aparecida; Sartoratto,Adilson.
The use of antibiotics in agriculture is limited when compared to their applications in human and veterinary medicine. On the
other hand, the use of antimicrobials in agriculture contributes to the drug resistance of human pathogens and has stimulated the
search for new antibiotics from natural products. Essential oils have been shown to exert several biological activities including
antibacterial and antifungal actions. The aim of this study was to determine the activity of 28 essential oils from medicinal plants
cultivated at CPMA (Medicinal and Aromatic Plants Collection), CPQBA/UNICAMP, against Colletotrichum gloeosporioides
(Penz.) Sacc., the anthracnose agent in yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg), as well as evaluating...
Tipo: Journal article
Palavras-chave: Yellow passion fruit; Colletotrichum gloeosporioides; Anthracnose; Antimicrobial activity; Minimal inhibitory
concentration.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100012
Multidrug-resistant tuberculosis among male inmates in Rio de Janeiro, Brazil
Provedor de dados: 58
Autores: Lourenço,Maria Cristina da Silva; Silva,Marlei Gomes da; Fonseca,Leila de Souza.
Susceptibility tests to six anti-tuberculosis drugs were performed on fifty-eight M. tuberculosis isolates obtained from tuberculous
inmates in the Male Penal Sanatorium, Rio de Janeiro, Brazil. The rate of resistant tuberculosis was higher than that observed in
the community. The overall resistance rate was 17.2% and 3.4% of the isolates were multi-drug resistant.
Tipo: Journal article
Palavras-chave: Multi-drug resistance; M. tuberculosis; Prisons.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100005
Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle
Provedor de dados: 58
Autores: Cleff,Marlete Brum; Lima,Alzira Pacheco de; Faria,Renata Osório de; Meinerz,Ana Raquel Mano; Antunes,Tatiana de
Ávila; Araújo,Flávia Biasoli de; Nascente,Patrícia da Silva; Nobre,Márcia de Oliveira; Meireles,Mário Carlos Araújo.
Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and other mucosa in mammalians. Candida
spp has been frequently isolated from domestic and sylvan animals. Yeast isolation from vaginal mucus is frequent in women,
especially of Candida genus. In canine females, however, studies about vaginal fungal microbiota are rare and the connection with
estrous cycle is unknown. The purpose of this study was to isolate yeast 5 of Candida genus from healthy canine females and
identify the isolated species, establishing their connection with estrous cycle phases. Two hundred twenty-four samples taken
from vaginal mucus of 14 canine females were analyzed in this study. Candida spp was observed in 83 (37%) from these samples;
nine...
Tipo: Journal article
Palavras-chave: Candida spp; Canine females; Estrous cycle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200018
Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent
Provedor de dados: 58
Autores: Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal.
Enzymes, and particularly proteases, have become an important and indispensable part of industrial processes such as laundry
detergents, pharmaceuticals and food products. Detergents such as Tide®, Ariel® and Biz® contain proteolytic enzymes, most of
them produced by members of the genus Bacillus. This paper describes the compatibility of protease produced by the thermophilic
Bacillus sp, with commercial laundry detergent. Stability studies indicated that this enzyme retained about 95% and 74% of its
maximum activity after 1h at 60ºC in the presence of glycine in combination with MnSO4 and CaCl2, respectively. No inhibitory
effect was observed at 1.0-5.0 mM of EDTA. Triton X-100 inhibited the enzyme in all the concentrations tested. The enzyme was
unstable...
Tipo: Journal article
Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.; Detergents.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300020
Molecular characterization of bacterial populations of different soils
Provedor de dados: 58
Autores: Pereira,Rodrigo Matheus; Silveira,Érico Leandro da; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida
Nascimbém; Val-Moraes,Silvana Pompéia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Until recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the
bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by
intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by
man; both located in Guaíra, São Paulo State, Brazil. Using specific primers, 16S rRNA genes from metagenomic DNA in both
soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S
rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla:
Acidobacteria,...
Tipo: Journal article
Palavras-chave: Metagenome; Microbial diversity; 16S rRNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400007
Growth and biopigment accumulation of cyanobacterium Spirulina platensis at different light
intensities and temperature
Provedor de dados: 58
Autores: Kumar,Manoj; Kulshreshtha,Jyoti; Singh,Gajendra Pal.
In order to find out optimum culture condition for algal growth, the effect of light irradiance and temperature on growth rate,
biomass composition and pigment production of Spirulina platensis were studied in axenic batch cultures. Growth kinetics of
cultures showed a wide range of temperature tolerance from 20 ºC to 40 ºC. Maximum growth rate, cell production with
maximum accumulation of chlorophyll and phycobilliproteins were found at temperature 35 ºC and 2,000 lux light intensity. But
with further increase in temperature and light intensity, reduction in growth rate was observed. Carotenoid content was found
maximum at 3,500 lux. Improvement in the carotenoid content with increase in light intensity is an adaptive mechanism of
cyanobacterium...
Tipo: Journal article
Palavras-chave: Spirulina platensis; Growth kinetics; Chlorophyll a; Carotenoid; Phycobiliproteins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300034
Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm
Provedor de dados: 58
Autores: Rosa,Juliana Pacheco da; Tibúrcio,Samyra Raquel Gonçalves; Marques,Joana Montezano; Seldin,Lucy; Coelho,Rosalie
Reed Rodrigues.
ABSTRACT Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing
bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this
antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study
the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was
six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A
reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments...
Tipo: Journal article
Palavras-chave: Streptomyces; Antimicrobial substance; Biofilm; Biocorrosion; Sulfate reducing bacteria.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300603
Hygienic-sanitary conditions of vegetables and irrigation water from kitchen gardens in the
municipality of Campinas, SP
Provedor de dados: 58
Autores: Simões,Marise; Pisani,Beatriz; Marques,Eneida Gonçalves Lemes; Prandi,Maria Angela Garnica; Martini,Maria
Helena; Chiarini,Paulo Flávio Teixeira; Antunes,José Leopoldo Ferreira; Nogueira,Ana Paula.
We examined samples of irrigation water and vegetables from kitchen gardens in Campinas, Brazil. The bacterial analysis
condemned 22.3% of the vegetable samples, and the parasitological examination condemned 14.5%. The criteria established by
the Brazilian legislation condemned 11.8% of the irrigation water samples. Parasites were significantly more frequent in
vegetables in the rainy season, while excessive fecal coliforms were more frequent in the dry season. A proper monitoring of the
irrigation water supply is important to avoid the contamination of vegetables.
Tipo: Journal article
Palavras-chave: Vegetables; Irrigation water; Bacteria; Parasites.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400015
Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the
city of Limeira, São Paulo
Provedor de dados: 58
Autores: Barrella,Karina Medici; Garrafa,Patrícia; Monezi,Telma Alves; Hársi,Charlotte Marianna; Salvi,Cleber; Violante,Paula
Alessandra B. Costa; Mehnert,Dolores Ursula.
The aim of this study was to verify the presence and annual distribution of adenoviruses and hepatitis A virus in domestic sewage
in the city of Limeira, São Paulo. Fifty samples with a volume of 8 liters each were collected weekly from December 2004 to
December 2005. The viruses were concentrated by filtration through positively charged ZP60S filter membranes, followed by
ultracentrifugation. Human adenoviruses (HAdV) were detected by PCR followed by nested-PCR and screening for species F was
done by restriction of the PCR product with TaqI endonuclease. Virus infectivity assays were performed by inoculation of
concentrates onto HEp-2 cell monolayers. RT-PCR was used for the detection of hepatitis A virus. HAdV were detected in all
samples, and 64% of...
Tipo: Journal article
Palavras-chave: Adenoviruses; Human adenovirus F; Hepatitis A virus; Sewage treatment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100017
Occurrence of killer Candida glabrata clinical isolates
Provedor de dados: 58
Autores: O,Arroyo-Helguera; Alejandro,De Las Penas; Irene,Castaño.
In this work we characterized the occurrence of killer activity in 64 Candida glabrata clinical isolates under different conditions.
We found that only 6.25 % of the clinical isolates tested were positive for killer activity against a Saccharomyces cerevisiae W303
sensitive strain. Sensitivity of killer activity to different values of pH and temperatures was analyzed. We found that the killer
activity presented by all isolates was resistant to every pH and temperature tested, although optimal activity was found at a range
of pH values from 4 to 7 and at 37ºC. We did not observe extrachromosomal genetic elements associated with killer activity in
any of the positive C. glabrata isolates. The killer effect was due to a decrease in viability and DNA...
Tipo: Journal article
Palavras-chave: Candida glabrata; Killer activity; Yeast; Saccharomyces.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300005
Endoglucanase production with the newly isolated Myceliophtora sp. i-1d3b in a packed bed solid
state fermentor
Provedor de dados: 58
Autores: Zanelato,A. I.; Shiota,V. M.; Gomes,E.; Silva,R. da; Thoméo,J. C.
This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the
fungus Myceliophtora sp. I-1D3busing a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium.
Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50ºC), initial
moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in
plastic bags were obtained using the substrate proportion of 7:3, 50ºC temperature, and 80% initial moisture content (878 U/grams
of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported.
For the...
Tipo: Journal article
Palavras-chave: Cellulase; Xylanase; Myceliophthora sp.; Packed bed; Solid state-fermentation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400038
Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity
markers
Provedor de dados: 58
Autores: Mori,Enio; Lara,Maria do Carmo C.S.H.; Cunha,Elenice M.S.; Villalobos,Eliana M.C.; Mori,Claudia M.C.;
Soares,Rodrigo M.; Brandão,Paulo E.; Fernandes,Wilson R.; Richtzenhain,Leonardo J.
Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA
polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular
variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood,
cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was
positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06
and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64
and ORF30 genes from three EHV-1...
Tipo: Journal article
Palavras-chave: Equid herpesvirus type 1; Equid Brazilian herpesvirus; ICP4 gene (ORF64); Glycoprotein D gene (ORF72);
DNA polymerase gene (ORF30).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200565
Free radical scavenging and antimicrobial properties of extracts of wild mushrooms
Provedor de dados: 58
Autores: Oyetayo,V.O.
Antioxidant and antimicrobial potentials of extracts obtained from four wild mushrooms, Termitomyces clypeatus (TCE),
Termitomyces robustus (TRE), Lentinus subnudus (LSE) and Lenzites species (LZE) collected in Nigeria were investigated. LSE
and LZE displayed good scavenging activity against 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and ferrous ion radicals at
concentration of 2 mg/mL. However, TRE and TCE exhibited better superoxide anion scavenging effect at 2 mg/mL. All extracts
(TCE, TRE, LSE and LZE) had comparable scavenging effect on hydroxyl radicals as butylated Hydroxytoluene (BHT) used as
control. Moreover, extracts from the wild mushrooms were able to inhibit the growth of all indicator organisms at concentrations
between 12.5 mg/mL to 100 mg/mL....
Tipo: Journal article
Palavras-chave: Wild mushrooms; Antimicrobial activity; Free radical scavenging.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200031
Bioprocessing of some agro-industrial residues for endoglucanase production by the new subsp.;
Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J
Provedor de dados: 58
Autores: El-Naggar,Noura El-Ahmady; Abdelwahed,Nayera A.M.; Saber,Wesam I.A.; Mohamed,Asem A.
The use of low cost agro-industrial residues for the production of industrial enzymes is one of the ways to reduce significantly
production costs. Cellulase producing actinomycetes were isolated from soil and decayed agricultural wastes. Among them, a
potential culture, strain NEAE-J, was selected and identified on the basis of morphological, cultural, physiological and
chemotaxonomic properties, together with 16S rDNA sequence. It is proposed that strain NEAE-J should be included in the
species Streptomyces albogriseolus as a representative of a novel sub-species, Streptomyces albogriseolus subsp. cellulolyticus
strain NEAE-J and sequencing product was deposited in the GenBank database under accession number JN229412. This organism
was tested for its...
Tipo: Journal article
Palavras-chave: Agro-industrial residues; Endoglucanase production; Streptomyces albogriseolus; 16S rRNA; Scanning electron
microscope.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200050
Optimization of zofimarin production by an endophytic fungus, Xylaria sp. Acra L38
Provedor de dados: 58
Autores: Chaichanan,Jirapan; Wiyakrutta,Suthep; Pongtharangkul,Thunyarat; Isarangkul,Duangnate; Meevootisom,Vithaya.
To optimize the medium for high zofimarin production, sucrose maltose, glucose, tryptone and peptone were used in an
orthogonal array design experiment, where the highest value of zofimarin produced was 25.6 µg/mL. This value was about 3
times higher than that obtained with Czapek yeast extract (CzYE) culture medium. A study with Plackett-Burman design showed
that sucrose, maltose, glucose and NaNO3 were significant factors in zofimarin production. Further studies using central
composite design (CCD) showed the significance of glucose and the interactions of these critical components affecting zofimarin
production. Multiple regression analysis of the data yielded a poor fit as shown by the mismatch of the model with these variable
factors. When a...
Tipo: Journal article
Palavras-chave: Zofimarin; Secondary metabolite; Medium optimization; Statistical methods; Endophytic fungus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100042
Alternative sanitization methods for minimally processed lettuce in comparison to sodium
hypochlorite
Provedor de dados: 58
Autores: Bachelli,Mara Lígia Biazotto; Amaral,Rívia Darla Álvares; Benedetti,Benedito Carlos.
Lettuce is a leafy vegetable widely used in industry for minimally processed products, in which the step of sanitization is the
crucial moment for ensuring a safe food for consumption. Chlorinated compounds, mainly sodium hypochlorite, are the most used
in Brazil, but the formation of trihalomethanes from this sanitizer is a drawback. Then, the search for alternative methods to
sodium hypochlorite has been emerging as a matter of great interest. The suitability of chlorine dioxide (60 mg L-1/10 min),
peracetic acid (100 mg L-1/15 min) and ozonated water (1.2 mg L-1 /1 min) as alternative sanitizers to sodium hypochlorite (150
mg L-1 free chlorine/15 min) were evaluated. Minimally processed lettuce washed with tap water for 1 min was used as a
control....
Tipo: Journal article
Palavras-chave: Food safety; Chlorine dioxide; Peracetic acid; Ozone; Minimal processing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300002
PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal
cheese commercialized in Goiânia, GO
Provedor de dados: 58
Autores: Carvalho,Rosangela Nunes; Oliveira,Antonio Nonato de; Mesquita,Albenones José de; Rezende,Cíntia Silva Minafra e;
Mesquita,Adriano Queiroz de; Romero,Rolando Alfredo Mazzoni.
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and
hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by
E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain
Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan
farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located
in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in
open air markets using ELISA,...
Tipo: Journal article
Palavras-chave: Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157®.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100002
Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) strains in ground
beef in São Paulo, Brazil
Provedor de dados: 58
Autores: Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina
Cabilio.
This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities
located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during
the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo
State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for
STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates
included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay.
STEC...
Tipo: Journal article
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032
Microbial diversity in Brazilian mangrove sediments: a mini review
Provedor de dados: 58
Autores: Ghizelini,Angela Michelato; Mendonça-Hagler,Leda Cristina Santana; Macrae,Andrew.
The importance and protection of mangrove ecosystems has been recognized in Brazilian Federal law since 1965. Being protected
in law, however, has not always guaranteed their protection in practice. Mangroves are found in coastal and estuarine locations,
which are prime real estate for the growth of cities, ports and other economic activities important for Brazilian development. In
this mini-review we introduce what mangroves are and why they are so important. We give a brief overview of the microbial
diversity found in mangrove sediments and then focus on diversity studies from Brazilian mangroves. We highlight the breadth
and depth of knowledge about mangrove microbial communities gained from studying Brazilian mangroves. We report on the
exciting...
Tipo: Journal article
Palavras-chave: Mangroves; Biodiversity; Bacteria; Fungi; Brazil; Conservation; Ecology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400002
Arcobacter butzleri an emerging enteropathogen: communication of two cases with chronic diarrhea
Provedor de dados: 58
Autores: Fernández,Heriberto; Krause,Sergio; Paz Villanueva,María.
The first two cases of chronic diarrhea due to Arcobacter butzleri in Chile are reported. The clinical findings, the absence of other
enteropathogens, virus or parasites, the epidemiological association between both patients, the treatment outcome and the fact that
A. butzleri was the only bacteria isolated, support the assumption that it was the etiological agent of these chronic diarrhea cases.
Tipo: Journal article
Palavras-chave: Arcobacter butzleri; Chronic diarrhea; Emerging pathogen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200008
Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease
Provedor de dados: 58
Autores: Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo.
Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen.
have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase,
polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores
dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with
predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%).
Tipo: Journal article
Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009
Detection of Ureaplasma spp. in semen samples from sheep in Brazil
Provedor de dados: 58
Autores: Santos,Sandra Batista dos; Souza Neto,Orestes Luiz de; Albuquerque,Pedro Paulo Feitosa de; Mota,André da Rocha;
Kim,Pomy de Cássia Peixoto; Moraes,Érica Paes Barreto Xavier de; Nascimento,Elmiro Rosendo do; Mota,Rinaldo Aparecido.
A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of
Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was
detected in 26.0% and Ureaplasma spp. in 12.0% of semen samples.
Tipo: Journal article
Palavras-chave: Mycoplasmas; Reproduction diseases; Semen; Sheep.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300040
Susceptibility of Saccharomyces cerevisiae and lactic acid bacteria from the alcohol industry to
several antimicrobial compounds
Provedor de dados: 58
Autores: Oliva-Neto,Pedro de; Yokoya,Fumio.
The antimicrobial effect of several products including commercial formulations currently used in sugar and alcohol factories was
determined by adapted MIC (Minimal Inhibitory Concentration) test on Saccharomyces cerevisiae and on natural contaminants
Lactobacillus fermentum and Leuconostoc mesenteroides. The MIC test by macrodilution broth method was adapted by
formulating of the culture medium with cane juice closely simulating industrial alcoholic fermentation must. Acid penicillin V
(MIC 0.10-0.20 µg/ml) and clindamycin (MIC 0.05-0.40 µg/ml) were most effective against bacterial growth in 24 h. Among the
chemicals, sulphite (MIC 10-40 µg/ml), nitrite (MIC <117 µg/ml) and copper sulphate (75-300 µg/ml) were the most effective.
Zinc and manganese...
Tipo: Journal article
Palavras-chave: Antimicrobial compound; MIC; S. cerevisiae; Lactic acid bacteria.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100003
Structural and Functional Analysis of Giant Strong Component of Bacillus thuringiensis Metabolic
Network
Provedor de dados: 58
Autores: Ding,D.W.; Ding,Y.R.; Li,L.N.; Cai,Y.J.; Xu,W.B.
The purpose of this work was to study the giant strong component (GSC) of B. thuringiensis metabolic network by structural and
functional analysis. Based on so-called "bow tie" structure, we extracted and studied GSC with its functional significance. Global
structural properties such as degree distribution and average path length were computed and indicated that the GSC is also a
small-world and scale-free network. Furthermore, the GSC was decomposed and functional significant for metabolism of these
divisions were investigated by comparing to KEGG metabolic pathways.
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Giant Strong Component; Metabolic network.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200036
Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by
multilocus enzyme electrophoresis
Provedor de dados: 58
Autores: Tahmourespour,Arezoo; Nabinejad,Abdolreza; Shirian,Hannaneh; Rosa,Edvaldo Antonio Ribeiro;
Tahmourespour,Sanaz.
This study was evaluated the clonal diversity of Streptococcus mutans in caries-free and caries-active subjects using MLEE.
Strains from caries-free subjects were grouped in a single taxon. Unrooted dendrogram showed that different strains clustered in
four different clades, also showed that more than one clonal type can be found in a same individual.
Tipo: Journal article
Palavras-chave: Streptococcus mutans; Multilocus enzyme electrophoresis; Caries free; Caries susceptible; Typing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300033
Partial characterization of ribosomal operons of Lactobacillus delbrueckii UFV H2b20
Provedor de dados: 58
Autores: Magalhães,Juliana Teixeira de; Floresta,Fernanda; Moraes,Célia Alencar de.
Ribosomal operons are great tools for microbe community characterization and for microorganisms relationship study,
particularly in the case of the acid lactic bacteria. The ribosomal operon of the probiotic strain Lactobacillus delbrueckii UFV
H2b20 was partially characterized. A genomic library of this strain was constructed and the clones with partial ribosomal operon
were sub-cloned using the shot-gun method for subsequent sequencing with the forward primer. The sequence analysis revealed
that the 3' end of the rDNA 16S was following by the short spacer region 1 (16S-23S) and that the 3' end of the rDNA 23S was
following by the short spacer region 2 (23S-5S), which preceded the rDNA 5S. In the flanking region of the rDNA 5S gene of this
operon rrn, a...
Tipo: Journal article
Palavras-chave: Ribosomal operons; Lactobacillus; TRNA; Acid lactic bacteria.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200014
Glucuronidase activity of Escherichia coli isolated from chicken carcasses
Provedor de dados: 58
Autores: Perin,Luana Martins; Yamazi,Anderson Keizo; Moraes,Paula Mendonça; Cossi,Marcus Vinícius Coutinho; Pinto,Paulo
Sérgio de Arruda; Nero,Luís Augusto.
To identify Escherichia coli through the production of β-D-glucuronidase (GUD), 622 suspect cultures were isolated
from chicken carcasses and plated in PetrifilmTM EC. Of these cultures, only 44 (7.1%) failed to produce GUD. This result
indicates the usefulness of GUD production for estimating E. coli populations in chicken.
Tipo: Journal article
Palavras-chave: Escherichia coli; Glucuronidase activity; Chicken.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300036
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different
sugarcane varieties
Provedor de dados: 58
Autores: Guedes,Helma V.; Santos,Samuel T. dos; Perin,Liamara; Teixeira,Kátia R. dos S.; Reis,Veronica M.; Baldani,José I.
A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in
Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP
techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose
were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis
showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis
was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species.
Neither effect...
Tipo: Journal article
Palavras-chave: Horizontal spread; Resistance plasmids; ESBL.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023
Postantibiotic effects and postantibiotic sub-MIC effects of tilmicosin, erythromycin and tiamulin on
erythromycin-resistant Streptococcus suis
Provedor de dados: 58
Autores: Wang,Liping; Zhang,Yuanshu.
The postantibiotic effects (PAEs) and postantibiotic sub-MIC effects (PA SMEs) of tilmicosin, erythromycin and tiamulin on
erythromycin-susceptible and erythromycin-resistant strains of Streptococcus suis (M phenotype) were investigated in vitro.
Tilmicosin and tiamulin induced significantly longer PAE and PA SME against both erythromycin-susceptible and
erythromycin-resistant strains than did erythromycin. The durations of PAE and PA SMEs were proportional to the concentrations
of drugs used for exposure. The PA SMEs were substantially longer than PAEs on S. suis (P<0.05) regardless of the
antimicrobial used for exposure. The results indicated that the PAE and PA SME could help in the design of efficient control
strategies for infection especially...
Tipo: Journal article
Palavras-chave: Postantibiotic effects; Postantibiotic sub-MIC effects; Tilmicosin; Tiamulin; Erythromycin;
Erythromycin-resistant Streptococcus suis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400033
Biodegradation of acetonitrile by cells of Candida guilliermondii UFMG-Y65 immobilized in alginate,
kappa-carrageenan and citric pectin
Provedor de dados: 58
Autores: Dias,João Carlos T.; Rezende,Rachel P.; Linardi,Valter R.
Different encapsulation matrices were tested for immobilized cells of Candida guilliermondii UFMG-Y65 used for acetonitrile
degradation. Acetonitrile degradation by free cells and cells immobilized in Ba-alginate, kappa-carrageenan and citric pectin was
studied. The rate of acetonitrile degradation was monitored for 120 h by measuring yeast growth and ammonia concentration.
Different alginate concentrations did not affect cell viability, but the period of incubation in BaCl2 solution reduced the number of
viable cells. Likewise, the gel nature and the matrix structure of the support resulting from the cell immobilization conditions were
of fundamental importance for biocatalyst activity and performance, affecting substantially the patterns of microbial...
Tipo: Journal article
Palavras-chave: Candida guilliermondii; Acetonitrile; Biodegradation; Immobilization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100015
Secondary metabolites from Diplodia maydis and Sclerotium rolfsii with antibiotic activity
Provedor de dados: 58
Autores: Nogueira,Marisa Alves; Diaz,Gaspar; Andrioli,William; Falconi,Fabiana André; Stangarlin,José Renato.
Ethyl acetate extracts of the phytopathogenic fungi Diplodia maydis and Sclerotium rolfsii presented antibacterial activity against
multidrug resistant bacteria isolated from patients in the University Hospital (HUOP-Unioeste, Brazil).
Tipo: Journal article
Palavras-chave: Diplodia maydis; Sclerotium rolfsii; Multidrug resistance; Phytopathogenic fungi; Secondary metabolites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100002
The synthesis of Phosphate-repressible alkaline phosphatase do not appear to be regulated by ambient
pH in the filamentous mould Neurospora crassa
Provedor de dados: 58
Autores: Nozawa,Sérgio R.; Thedei Jr.,Geraldo; Crott,Luciana S.P.; Barbosa,José E.; Rossi,Antonio.
In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded
Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould
secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not
functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pi starvation via
the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation
observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH
8.0 is...
Tipo: Journal article
Palavras-chave: Alkaline phosphatase; Ambient pH; Enzyme secretion; Neurospora crassa; Pi sensing.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100018
Analysis of moisture content, acidity and contamination by yeast and molds in Apis mellifera L. honey
from central Brazil
Provedor de dados: 58
Autores: Ananias,Karla Rubia; Melo,Adriane Alexandre Machado de; Moura,Celso José de.
The development of mold of environmental origin in honey affects its quality and leads to its deterioration, so yeasts and molds
counts have been used as an important indicator of hygiene levels during its processing, transportation and storage. The aim of this
study was to evaluate the levels of yeasts and molds contamination and their correlation with moisture and acidity levels in Apis
mellifera L. honey from central Brazil. In 20% of the samples, the yeasts and molds counts exceeded the limit established by
legislation for the marketing of honey in the MERCOSUR, while 42.8% and 5.7% presented above-standard acidity and moisture
levels, respectively. Although samples showed yeasts and molds counts over 1.0 x 10² UFC.g-1, there was no correlation
between...
Tipo: Journal article
Palavras-chave: Honey; Apis mellifera L.; Quality.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300003
Antifungal activity of different neem leaf extracts and the nimonol against some important human
pathogens
Provedor de dados: 58
Autores: Mahmoud,D.A; Hassanein,N.M; Youssef,K.A; Abou Zeid,M.A.
This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of
some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and
Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of
the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest
inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid
Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was
purified and chemically...
Tipo: Journal article
Palavras-chave: Azadirachta indica; Aqueous and organic extracts; HPLC; Fungal inhibitory effect.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300021
Draft genome sequences of five Pseudomonas syringae pv. actinidifoliorum strains isolated in France
Provedor de dados: 58
Autores: Cunty,Amandine; Cesbron,Sophie; Briand,Martial; Carrère,Sébastien; Poliakoff,Françoise; Jacques,Marie-Agnès;
Manceau,Charles.
ABSTRACT Pseudomonas syringae pv. actinidifoliorum causes necrotic spots on the leaves of Actinidia deliciosa and Actinidia
chinensis. P. syringae pv. actinidifoliorum has been detected in New Zealand, Australia, France and Spain. Four lineages were
previously identified within the P. syringae pv. actinidifoliorum species group. Here, we report the draft genome sequences of five
strains of P. syringae pv. actinidifoliorum representative of lineages 1, 2 and 4, isolated in France. The whole genomes of strains
isolated in New Zealand, representative of P. syringae pv. actinidifoliorum lineages 1 and 3, were previously sequenced. The
availability of supplementary P. syringae pv. actinidifoliorum genome sequences will be useful for developing molecular tools...
Tipo: Journal article
Palavras-chave: Pseudomonas syringae; Actinidia; Kiwifruit pathogen; Leaf necrotic spots.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300529
Bioassay for selection of biocontroller bacteria against bean common blight (Xanthomonas
axonopodis pv. phaseoli)
Provedor de dados: 58
Autores: Zanatta,Zarela G.C.N.; Moura,Andréa B.; Maia,Luciano C.; Santos,André S. dos.
Emphasis has been given on selection of micro-organism for biological control. However, in order to evaluate the biological
control potential of a great number of micro-organisms in a small period of time it is necessary to develop an efficient bioassay.
Seven hundred and sixty bacterial isolates from different habitats, were selected for compatibility with Rhizobium leguminosarum
bv. phaseoli (SEMIA 4077 e SEMIA 4080). Among them 596 isolates were ineffective against both rhizobia. Bean seeds
immersed in suspension of each one of these isolates were agitated for 5 hours at 10ºC and sowed in non-sterilized soil. The plants
were kept in greenhouse. After the development of cotyledonary and primary leaves, these were removed and bioassayed for
Xanthomonas...
Tipo: Journal article
Palavras-chave: Biological control; Antagonism; Phaseolus vulgaris; Microbiolization.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300024
Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples
from dairy farms
Provedor de dados: 58
Autores: Vicente,Hinig Isa Godoy; Amaral,Luiz Augusto do; Cerqueira,Aloysio de Mello Figueiredo.
The objective of this study was to determine the prevalence of Shigatoxigenic Escherichia coli (STEC) and STEC serogroups
O157, O111 and O113 in feces, water and milk sampled in dairy farms in Jaboticabal, SP, Brazil. Feces (n=454), water (n=54)
and milk samples (n=30) were collected from 10 herds and assessed for the presence of the virulence genes stx1, stx2 and eae by
polymerase chain reaction (PCR). All stx and eae positive samples were submitted to a second PCR reaction targeting the
sequences rfb O157, rfb O111 and rfb O113. High prevalence of stx was detected (59.9%) in fecal samples, whereas the
prevalence of sequences rfb O157, rfb O111 and rfb O113 was 18.9%, 3.3% and 30.4%, respectively. All sequences were detected
more frequently in calves and...
Tipo: Journal article
Palavras-chave: Shigatoxigenic; Escherichia coli; Milk; Water; Bovine feces.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300003
Caracterization of Aujeszky's disease virus isolated from South Brazil in the last twenty years by
restriction enzyme analysis
Provedor de dados: 58
Autores: Schaefer,Rejane; Zanella,Janice Ciacci; Mores,Nelson; Pan,Kleitton Adolfo; Dambros,Régia Maria Feltrim;
Schiochet,Marisete Fracasso; Coldebella,Michele.
Aujeszky's disease virus (ADV) belongs to Herpesviridae family and is an important etiological agent which infects pigs causing
economic losses in swine producing countries worldwide and international trade restrictions to products of swine origin. An
eradication program for ADV was established in Santa Catarina State since 2001. The last outbreak was reported in July 2004 and
since then none has been reported. The disease has been controlled with the use of a genetic modified vaccine and elimination of
seropositives. Aiming the characterization of ADV isolated in the South of Brazil in the last twenty years (1983-2003), a
retrospective study based on the genomic analysis of the isolates through a digestion of viral genomic DNA with restriction
enzyme Bam...
Tipo: Journal article
Palavras-chave: Aujeszky's disease virus; Swine; Restriction endonuclease analysis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300035
Determination of the antibacterial activity of crude extracts and compounds isolated from Hortia
oreadica (Rutaceae) against oral pathogens
Provedor de dados: 58
Autores: Severino,Vanessa Gisele Pasqualotto; Silva,Maria Fátima das Graças Fernandes da; Lucarini,Rodrigo; Montanari,Lilian
Bueno; Cunha,Wilson Roberto; Vinholis,Adriana Helena Chicharo; Martins,Carlos Henrique Gomes.
Extracts from Hortia oreadica afforded four dihydrocinnamic acid derivatives, isolated from the n-hexane extract, as well as
limonoid guyanin and the furoquinoline alkaloid dictamnine, both isolated from the dichloromethane extract. The extracts and the
isolated compounds were tested against some oral pathogens, so as to investigate their antibacterial activity. The results showed
that the n-hexane extract and the compound dictamnine are the most active against the selected microorganisms.
Tipo: Journal article
Palavras-chave: Hortia oreadica; Rutaceae; Antibacterial activity; MIC; Oral pathogens.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300015
Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escherichia vulneris
Provedor de dados: 58
Autores: Shobrak,Mohammed Y.; Abo-Amer,Aly E.
Emergence and distribution of multi-drug resistant (MDR) bacteria in environments pose a risk to human and animal health. A
total of 82 isolates of Escherichia spp. were recovered from cloacal swabs of migrating and non-migrating wild birds. All bacterial
isolates were identified and characterized morphologically and biochemically. 72% and 50% of isolates
recovered from non-migrating and migrating birds, respectively, showed positive congo red dye binding (a virulence factor). Also,
hemolysin production (a virulence factor) was showed in 8% of isolates recovered from non-migrating birds and
75% of isolates recovered from migrating birds. All isolates recovered from non-migrating birds were found resistant to
Oxacillin while...
Tipo: Journal article
Palavras-chave: E. coli; E. vulneris; Multi-drug resistance (MDR); Migrating and non-migrating birds; Congo red binding;
Hemolysin; API 20E; 16S rRNA; Plasmid profile.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400010
Chlorhexidine: beta-cyclodextrin inhibits yeast growth by extraction of ergosterol
Provedor de dados: 58
Autores: Teixeira,K. I. R.; Araújo,P. V.; Sinisterra,R. D.; Cortés,M. E.
Chlorhexidine (Cx) augmented with beta-cyclodextrin (β-cd) inclusion compounds, termed Cx:β-cd
complexes, have been developed for use as antiseptic agents. The aim of this study was to examine the interactions of
Cx:β-cd complexes, prepared at different molecular ratios, with sterol and yeast membranes. The Minimal Inhibitory
Concentration (MIC) against the yeast Candida albicans (C.a.) was determined for each complex; the MICs were found to range
from 0.5 to 2 µg/mL. To confirm the MIC data, quantitative analysis of viable cells was performed using trypan blue staining.
Mechanistic characterization of the interactions that the Cx:β-cd complexes have with the yeast membrane and
assessment of membrane morphology following...
Tipo: Journal article
Palavras-chave: Chlorhexidine β -cyclodextrin membrane-drug interactions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200047
Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas
Gerais, Brazil
Provedor de dados: 58
Autores: Torres,Adalgisa Ribeiro; Cursino,Luciana; Muro-Abad,Júpiter Israel; Gomes,Eliane Aparecida; Araújo,Elza Fernandes
de; Hungria,Mariangela; Cassini,Sérvio Túlio Alves.
We characterized indigenous common bean rhizobia from five districts of the state of Minas Gerais, Brazil. The isolates were
trapped by two common bean varieties, the Mineiro Precoce (Andean origin) and Ouro Negro (Mesoamerican origin). Analysis by
BOX-PCR of selected isolates detected a high level of genetic diversity.
Tipo: Journal article
Palavras-chave: BOX-PCR; Phaseolus vulgaris; Rhizobium.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400016
Isolation and preliminary characterization of a o-nitrobenzaldehyde-degrading Alcaligenes sp. ND1
Provedor de dados: 58
Autores: Fang-Bo,Yu; Li-Bo,Guan; Shan,Zhou.
This paper reports the isolation and characterization of a new o-nitrobenzaldehyde (ONBA)-degrading bacterium, Alcaligenes sp.
ND1. ND1 degraded almost all ONBA (100 mg L-1) in M9 medium within 36 hours. The key enzyme(s) involved in the initial
biodegradation was a constitutively intracellular enzyme(s). This bacterium has great potential utility for bioremediation.
Tipo: Journal article
Palavras-chave: Biodegradation; O-nitrobenzaldehyde.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400033
Membrane fluidity and lipid composition of fluconazole resistant and susceptible strains of Candida
albicans isolated from diabetic patients
Provedor de dados: 58
Autores: Mishra,Nagendra N.; Prasad,Tulika; Sharma,Neeraj; Gupta,Dwijendra K.
Ten clinical isolates of Candida albicans, five strains belonging to each of fluconazole resistant and susceptible groups isolated
from diabetic patients, were studied for the membrane fluidity and lipid composition. Compared to fluconazole susceptible strains,
fluconazole resistant ones exhibited enhanced membrane fluidity as measured by fluorescence polarization technique. The
increased membrane fluidity was reflected in the decreased p-values exhibited by the resistant strains. On the other hand,
susceptible isolates contained higher amount of ergosterol, almost twice as compared to resistant isolates which might have
contributed to their lower membrane fluidity. However, no significant alteration was observed in the phospholipid and fatty acid...
Tipo: Journal article
Palavras-chave: Membrane fluidity; Lipid composition; Azole resistance; Diabetic patients.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200004
Filamentous fungi and media for cellulase production in solid state cultures
Provedor de dados: 58
Autores: Kilikian,B.V.; Afonso,L.C.; Souza,T.F.C.; Ferreira,R.G.; Pinheiro,I.R.
Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a
sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for
biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using
sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80%
moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the
production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not
respond as strongly...
Tipo: Journal article
Palavras-chave: Cellulase; Productivity; Solid media; Sugarcane bagasse; Myceliophtora sp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100041
Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks
occurred in southern Brazil
Provedor de dados: 58
Autores: Paula,Cheila Minéia Daniel de; Geimba,Mercedes Passos; Amaral,Patrícia Heidrich do; Tondo,Eduardo Cesar.
Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to
investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks
occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were
isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification
of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains
(6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains...
Tipo: Journal article
Palavras-chave: Shigellosis; Shigella; Antimicrobial; PCR-Ribotyping; RS/Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400015
Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus
murinus in the digestive tract of gnotobiotic mice
Provedor de dados: 58
Autores: Vasconcelos,Ana Luiza Sampaio; Nicoli,Jacques Robert; Nardi,Regina Maria Drummond.
In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was
evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a
gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83
days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo
against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of
Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract,
following...
Tipo: Journal article
Palavras-chave: Antagonism; Salmonella; Lactobacillus; Gnotobiotic mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc
flour industry
Provedor de dados: 58
Autores: Freitas,Telma Luisa de; Monti,Rubens; Contiero,Jonas.
GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was
examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained
with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High
enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an
optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however,
Hg+ and Cu+ showed a strong enzyme inhibition.
Tipo: Journal article
Palavras-chave: Bacillus alkalophilic CGII; CGTase; Beta-cyclodextrin; Starch hydrolysis; Enzyme activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015
Occurrence of Salmonella spp. in broiler chicken carcasses and their susceptibility to antimicrobial
agents
Provedor de dados: 58
Autores: Duarte,Dalila Angélica Moliterno; Ribeiro,Aldemir Reginato; Vasconcelos,Ana Mércia Mendes; Santos,Sylnei Barros;
Silva,Juliana Vital Domingos; Andrade,Patrícia Lúcia Arruda de; Falcão,Lúcia Sadae Pereira da Costa de Arruda.
The present study was carried out to evaluate the occurrence of Salmonellae in broiler chicken carcasses and to determine the
antimicrobial resistance profile of the isolated strains. Twenty-five out of the 260 broiler chicken carcasses samples (9.6%) were
positive for Salmonella. S. Enteritidis was the most frequent serovar. Nineteen Salmonella isolates were tested for antimicrobial
resistance, and the results indicated that 94.7% were resistant to at least one antimicrobial agent. Resistance to streptomycin
(73.7%), nitrofurantoin (52.3%), tetracycline (31.6%), and nalidixic acid (21%) were the prevalent amongst Salmonella strains
tested.
Tipo: Journal article
Palavras-chave: Salmonella; Broiler chicken carcasses; Antimicrobial resistance; Salmonella.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300020
A new PCR approach for the identification of Fusarium graminearum
Provedor de dados: 58
Autores: Biazio,Gleison Ricardo de; Leite,Gabriela Guimarães Sousa; Tessmann,Dauri José; Barbosa-Tessmann,Ione Parra.
The main objective of this work was to develop a PCR protocol for the identification of Fusarium graminearum, based on a pair of
primers targeted to a segment of the 3' coding region of the gaoA gene that codes for the enzyme galactose oxidase (GO). This
region has low homology with the same region of GO genes from other fungi. Genomic DNA from 17 strains of Fusarium spp.
isolated from diseased cereals, from several other Fusarium species, and from other fungi genera was analyzed in a PCR assay
using this primer set. The 17 strains of Fusarium spp. were also analyzed for the GO enzyme production in submerse fermentation
in a new formulated liquid medium. All strains that were morphologically and molecularly identified as F. graminearum were able
to secrete...
Tipo: Journal article
Palavras-chave: Fusarium graminearum; Galactose oxidase; GaoA gene; Molecular identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300028
Comparison of phenotypic and molecular tests to identify lactic acid bacteria
Provedor de dados: 58
Autores: Moraes,Paula Mendonça; Perin,Luana Martins; Silva Júnior,Abelardo; Nero,Luís Augusto.
Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA
sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic
approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
Tipo: Journal article
Palavras-chave: Lactic acid bacteria; Identification; 16sDNA; PCR; Biolog; API.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100015
Comparing how land use change impacts soil microbial catabolic respiration in Southwestern Amazon
Provedor de dados: 58
Autores: Mazzetto,Andre Mancebo; Feigl,Brigitte Josefine; Cerri,Carlos Eduardo Pellegrino; Cerri,Carlos Clemente.
Abstract Land use changes strongly impact soil functions, particularly microbial biomass diversity and activity. We hypothesized
that the catabolic respiration response of the microbial biomass would differ depending on land use and that these differences
would be consistent at the landscape scale. In the present study, we analyzed the catabolic response profile of the soil microbial
biomass through substrate-induced respiration in different land uses over a wide geographical range in Mato Grosso and Rondônia
state (Southwest Amazon region). We analyzed the differences among native areas, pastures and crop areas and within each land
use and examined only native areas (Forest, Dense Cerrado and Cerrado), pastures (Nominal, Degraded and Improved) and crop...
Tipo: Journal article
Palavras-chave: Amazon region; Land use change; Catabolic profile; Microbial communities.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100063
Isolation and characteristics of lactic acid bacteria isolated from ripe mulberries in Taiwan
Provedor de dados: 58
Autores: Chen,Yi-sheng; Wu,Hui-chung; Yanagida,Fujitoshi.
The objective of this study was to isolate, characterize, and identify lactic acid bacteria (LAB) from ripe mulberries collected in
Taiwan. Ripe mulberry samples were collected at five mulberry farms, located in different counties of Taiwan. Eighty-eight
acid-producing cultures were isolated from these samples, and isolates were divided into classes first by phenotype, then into
groups by restriction fragment length polymorphism (RFLP) analysis and sequencing of 16S ribosomal DNA (rDNA). Phenotypic
and biochemical characteristics led to identification of four bacterial groups (A to D). Weissella cibaria was the most abundant
type of LAB distributed in four mulberry farms, and Lactobacillus plantarum was the most abundant LAB found in the remaining
farm....
Tipo: Journal article
Palavras-chave: Lactic acid bacteria; Mulberry; Weissella cibaria; Bacteriocin; Taiwan.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400010
Antifungal activity of the ethanolic extracts of Punica granatum L. and evaluation of the
morphological and structural modifications of its compounds upon the cells of Candida spp.
Provedor de dados: 58
Autores: Anibal,Paula Cristina; Peixoto,Iza Teixeira Alves; Foglio,Mary Ann; Höfling,José Francisco.
Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as
pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass
Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel
showed activity against Candida spp., with MICs of 125 µg/mL. The effect of pericarp and peel extracts upon the morphological
and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the
visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained
revealed potential...
Tipo: Journal article
Palavras-chave: Punica granatum; Punicalagin; Antifungal activity; Candida spp.; Electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300027
Bacterial diversity in relatively pristine and anthropogenically-influenced mangrove ecosystems (Goa,
India)
Provedor de dados: 58
Autores: Fernandes,Sheryl Oliveira; Kirchman,David L.; Michotey,Valérie D.; Bonin,Patricia C.; LokaBharathi,P.A.
To appreciate differences in benthic bacterial community composition at the relatively pristine Tuvem and the
anthropogenically-influenced Divar mangrove ecosystems in Goa, India, parallel tag sequencing of the V6 region of 16S rDNA
was carried out. We hypothesize that availability of extraneously-derived anthropogenic substrates could act as a stimulatant but
not a deterrent to promote higher bacterial diversity at Divar. Our observations revealed that the phylum Proteobacteria was
dominant at both locations comprising 43-46% of total tags. The Tuvem ecosystem was characterized by an abundance
of members belonging to the class Deltaproteobacteria (21%), ~ 2100 phylotypes and 1561 operational taxonomic units
(OTUs) sharing >...
Tipo: Journal article
Palavras-chave: Bacteria; Denitrification; Diversity; Mangroves; Sediments.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400006
Prevention of Salmonella infection by contact using intestinal flora of adult birds and/or a mixture of
organic acids
Provedor de dados: 58
Autores: Oliveira,Gláucia Helaine de; Berchieri Junior,Angelo; Barrow,Paul A.
This study was carried out to assess the ability of competitive exclusion and a mixture of organic acids to prevent Salmonella
infection by contact between newly hatched chicks. A bird infected with Salmonella was placed in a box containing non-infected
birds, previously treated with a broth culture of faeces of adult birds (CE) and/or a mixture of organic acids. The number of
Salmonella organisms in the caeca of the contact birds was estimated at 4 and 8 days post-challenge. The birds were infected with
Salmonella Typhimurium, Salmonella Enteritidis (both repeated 5 times), Salmonella Agona and Salmonella Infantis (3
repetitions). The same approach was used to test the mixture of organic acids alone. In this case the birds received feed containing
0.8% of...
Tipo: Journal article
Palavras-chave: Salmonella; Competitive exclusion; Organic acids; By-contact infection; Poultry.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200011
Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145
Provedor de dados: 58
Autores: Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei.
In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile.
Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using
corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming
at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing
out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals
on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl
and urea, both...
Tipo: Journal article
Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011
Evaluation of tests to predict metallo-β-lactamase in cystic fibrosis (CF) and non-(CF)
Pseudomonas
Provedor de dados: 58
Autores: Perez,Leandro Reus Rodrigues; Limberger,Mariana Fagundes; Costi,Ricardo; Dias,Cícero Armídio Gomes;
Barth,Afonso Luís.
Double disks synergy test (DDST) and combined disks test (CD) were evaluated to predict the presence of
metallo-β-lactamase in 70 Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis
patients. DDST CAZ-EDTA 1 cm and CD IMP-EDTA tests showed the best accuracy (94.3%). Furthermore, for other
combinations, accuracy unsatisfactory was obtained.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa metallo-β -lactamase phenotypic tests.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300011
Clinical significance of the isolation of Candidaspecies from hospitalized patients
Provedor de dados: 58
Autores: Magalhães,Yankee C.; Bomfim,Maria Rosa Q.; Melônio,Luciane C.; Ribeiro,Patrícia C.S.; Cosme,Lécia M.;
Rhoden,Cristianne R.; Marques,Sirlei G.
In this study, we isolated and phenotypically identified 108 yeast strains from various clinical specimens collected from 100
hospitalized patients at three tertiary hospitals in São Luís-Maranhão, Brazil, from July to December 2010. The isolates were
analyzed for their susceptibility to four of the most widely used antifungal agents in the surveyed hospitals, amphotericin B,
fluconazole, 5-flucytosine and voriconazole. The species identified were Candida albicans (41.4%), Candida tropicalis (30.1%),
C. glabrata (7.4%), Candida parapsilosis(5.5%), Candida krusei (4.6%), Cryptococcus neoformans (4.6%), Trichosporonspp.
(3.7%), Candida norvegensis (0.9%), Rhodotorula glutinis (0.9%) and Pichia farinosa (0.9%). A higher isolation rate was
observed in the...
Tipo: Journal article
Palavras-chave: Fungal infections; Susceptibility profile; Nosocomial infections.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100117
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese
ripening
Provedor de dados: 58
Autores: Mata,Gardênia Márcia Silva Campos; Martins,Evandro; Machado,Solimar Gonçalves; Pinto,Maximiliano Soares;
Carvalho,Antônio Fernandes de; Vanetti,Maria Cristina Dantas.
ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to
evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These
methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on
laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in
cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in
terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by
Lateral Flow...
Tipo: Journal article
Palavras-chave: Listeria; Raw milk cheese; Alternative methods.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749
Peptidolytic microbial community of methanogenic reactors from two modified UASBs of brewery
industries
Provedor de dados: 58
Autores: Díaz,C.; Baena,S.; Patel,B.K.C.; Fardeau,M.L.
We studied the peptide-degrading anaerobic communities of methanogenic reactors from two mesophilic full-scale modified
upflow anaerobic sludge blanket (UASB) reactors treating brewery wastewater in Colombia. Most probable number (MPN)
counts varied between 7.1 x 10(8) and 6.6 x 10(9) bacteria/g volatile suspended solids VSS (Methanogenic Reactor 1) and 7.2 x
10(6) and 6.4 x 10(7) bacteria/g (VSS) (Methanogenic Reactor 2). Metabolites detected in the highest positive MPN dilutions in
both reactors were mostly acetate, propionate, isovalerate and, in some cases, negligible concentrations of butyrate. Using the
highest positive dilutions of MPN counts, 50 dominant strains were isolated from both reactors, and 12 strains were selected for
sequencing their 16S...
Tipo: Journal article
Palavras-chave: Anaerobic peptide-degrading bacteria; MPN; UASB; 16S rRNA gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300022
Optimization of growth and bacteriocin production by Lactobacillus sakei subsp. sakei2a
Provedor de dados: 58
Autores: Malheiros,Patrícia S.; Sant’Anna,Voltaire; Todorov,Svetoslav D.; Franco,Bernadette D.G.M.
Lactobacillus sakei subsp. sakei 2a is a bacteriocinogenic lactic acid bacterium isolated from Brazilian pork sausage, capable of
inhibiting the growth of microbial pathogens, mainly Listeria monocytogenes. In order to optimize bacteriocin production for
industrial applications, this study evaluated the effect of supplementation of MRS broth with glucose, Tween 20, Tween 80,
sodium citrate, potassium chloride and cysteine, and effect of the initial pH and temperature of incubation of the medium on
production of bacteriocins by L. sakei 2a. Adding glucose and Tween 20 to the medium, an initial pH of 5.0 or 5.5, and incubation
temperatures of 25 °C or 30 °C resulted to the highest bacteriocin yields. Thus, a 24 factorial design with the four variables was...
Tipo: Journal article
Palavras-chave: Lactobacillus sakei; Bacteriocin; Response surface methodology; Optimization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300825
Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtilis in three
vegetables
Provedor de dados: 58
Autores: Adesemoye,A.O.; Obini,M.; Ugoji,E.O.
Our objective was to compare some plant growth promoting rhizobacteria (PGPR) properties of Bacillus subtilis and
Pseudomonas aeruginosa as representatives of their two genera. Solanum lycopersicum L. (tomato), Abelmoschus esculentus
(okra), and Amaranthus sp. (African spinach) were inoculated with the bacterial cultures. At 60 days after planting, dry biomass
for plants treated with B. subtilis and P. aeruginosa increased 31% for tomato, 36% and 29% for okra, and 83% and 40% for
African spinach respectively over the non-bacterized control. Considering all the parameters tested, there were similarities but no
significant difference at P < 0.05 between the overall performances of the two organisms.
Tipo: Journal article
Palavras-chave: PGPR; Pseudomonas aeruginosa; Bacillus subtilis; Growth promotion.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300003
Use of anaerobic cecal microflora, lactose and acetic acid for the protection of broiler chicks against
experimental infection with Salmonella Typhimurium and Salmonella enteritidis
Provedor de dados: 58
Autores: Andreatti Filho,Raphael Lucio; Silva,Edir Nepomuceno da; Ribeiro,Aldemir Reginato; Kondo,Nancy; Curi,Paulo
Roberto.
The effects of treatment with anaerobic cecal microflora (ACM) and/or lactose and/or acetic acid on systemic and digestive tract
of broiler chicks infection with Salmonella Typhimurium and S. Enteritidis were studied. ACM was used without previous
bacterial identification. Treatment with ACM contributed to the resistance of broiler chicks to infection with Salmonella spp. The
infections were more persistent in the cecum, rectum and crops in decreasing order of intensity. The infections were also
self-limiting since treated and control lots presented similar infection rates at the end of the experiments. Alone or in combination
with lactose, ACM reduced the colonization of the digestive tract of broiler chicks by S. Typhimurium and S. Enteritidis. The...
Tipo: Journal article
Palavras-chave: Salmonella Typhimurium; S. Enteritidis; Anaerobic cecal microflora; Competitive exclusion; Lactose; Acetic
acid; Broiler chicks.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200009
Natural antibodies in paracoccidioidomycosis
Provedor de dados: 58
Autores: Unterkircher,Carmelinda S.; Leão,Mariella V.P.; Blotta,Maria Heloisa S.L.; Camargo,Zoilo Pires de.
Recent attention has been focused on the natural antibodies as a component of natural immunity and as integral part of the
idiotypic network. However, their functional role in different infections has rarely been studied. This work was undertaken to
investigate the presence of natural antibodies in paracoccidioidomycosis (PCM). In addition, we analyzed anti-P. brasiliensis
antibodies and their distribution in IgG subclasses in order to acquire better knowledge about the humoral immune response in this
mycosis. Our findings show that the natural antibody response is not very much increased in PCM when compared with other
parasite infections and this response is restricted to a few specificities, suggesting that P. brasiliensis moderately triggers CD5+
B...
Tipo: Journal article
Palavras-chave: Natural antibodies; Paracoccidioides brasiliensis; Paracoccidioidomycosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100009
Antibacterial activity of medicinal plant extracts
Provedor de dados: 58
Autores: Ushimaru,Priscila Ikeda; Silva,Mariama Tomaz Nogueira da; Di Stasi,Luiz Claudio; Barbosa,Luciano; Fernandes
Junior,Ary.
The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against
Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus and Enterococcus sp. The methanolic extract of Caryophyllus
aromaticus presented the highest anti-S. aureus activity and was effective against all bacterial strains tested.
Tipo: Journal article
Palavras-chave: Medicinal plants; Antibacterial activity; Minimal Inhibitory Concentration.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400024
Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous
Cultures of Immobilized Bacilluslicheniformis strain-R
Provedor de dados: 58
Autores: Berekaa,Mahmoud M.; El Aassar,Samy A.; El-Sayed,Samia M.; EL Borai,Aliaa M.
Production of Polyglutamate (PGA) biopolymer by immobilized Bacilluslicheniformis strain-R was intensively investigated.
Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells
in alginate-agar led optimal PGA production (36.75 g/l), with 1.32- and 2.18-fold increase in comparison with alginate- or
K-carrageenan-immobilized cells, respectively. During semicontinuous cultivation of agar-alginate gel-cell mixture, production of
PGA by 10 ml mixture was increased from 2nd to 3rd run whereas, increased till the 4th run using 15ml mixture. Adsorption was
the most suitable immobilization technique for production of PGA and the sponge cubes was the preferred matrix recording 43.2
g/l of PGA...
Tipo: Journal article
Palavras-chave: Poly-g-glutamate; Bacillu slicheniformis; Immobilization; Bioreactor; Semicontinuous production.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400001
Laccase produced by a thermotolerant strain of Trametes trogii LK13
Provedor de dados: 58
Autores: Yan,Jinping; Chen,Yuhui; Niu,Jiezhen; Chen,Daidi; Chagan,Irbis.
Thermophilic and thermotolerant micro-organisms strains have served as the natural source of industrially relevant and
thermostable enzymes. Although some strains of the Trametes genus are thermotolerant, few Trametes strains were studied at the
temperature above 30 °C until now. In this paper, the laccase activity and the mycelial growth rate for Trametes trogii LK13 are
superior at 37 °C. Thermostability and organic cosolvent tolerance assays of the laccase produced at 37 °C indicated that the
enzyme possessed fair thermostability with 50% of its initial activity at 80 °C for 5 min, and could remain 50% enzyme activity
treated with organic cosolvent at the concentration range of 25%–50% (v/v). Furthermore, the test on production of
laccase and...
Tipo: Journal article
Palavras-chave: Trametes trogii; Thermotolerant fungi; Laccase; Thermostability; Organic cosolvent tolerance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100059
Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana
L.
Provedor de dados: 58
Autores: De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha;
Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner.
ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana
was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected
for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant
inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene
showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and
Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate,
and had nitrogenase...
Tipo: Journal article
Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587
Antibacterial and Antioxidant Activities of Acid and Bile Resistant Strains of Lactobacillus
fermentum Isolated from Miang
Provedor de dados: 58
Autores: Klayraung,Srikanjana; Okonogi,Siriporn.
Miang is a kind of traditional fermented tea leaves, widely consumed in northern Thailand as a snack. It contains several kinds of
Lactobacilli spp. The aim of this study was to isolate strains of Lactobacillus fermentum from miang and to investigate their
antibacterial and antioxidant activities. The agar spot and well assays were used for determination of antibacterial power. The
antibacterial mechanism was investigated by cell morphologic change under scanning electron microscope (SEM). Antioxidant
activity was studied by means of free radical scavenging and ferric reducing power assays. The acid and bile screening tests
indicated that L. fermentum FTL2311 and L. fermentum FTL10BR presented antibacterial activity against several pathogenic
bacteria:...
Tipo: Journal article
Palavras-chave: Lactobacillus fermentum; Miang; Bile resistance; Antibacterial activity; Antioxidant activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400005
Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for
biomass-degrading enzyme production
Provedor de dados: 58
Autores: Santos,Bruna Silveira Lamanes dos; Gomes,Arthur Filipe Sousa; Franciscon,Emanuele Giuliane; Oliveira,Jean Maikon
de; Baffi,Milla Alves.
Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian
Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four
species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the
isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three
strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus
(SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at
pH 3.5 and 55 °C...
Tipo: Journal article
Palavras-chave: β -glucosidase β -xylosidase xylanase Aspergillus sugarcane bagasse.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300903
Seasonality of viral respiratory infections in Southeast of Brazil: the influence of temperature and air
humidity
Provedor de dados: 58
Autores: Gardinassi,Luiz Gustavo; Simas,Paulo Vitor Marques; Salomão,João Batista; Durigon,Edison Luiz; Trevisan,Dirce
Maria Zanetta; Cordeiro,José Antonio; Lacerda,Mauricio Nogueira; Rahal,Paula; Souza,Fátima Pereira de.
Viruses are the major cause of lower respiratory tract infections in childhood and the main viruses involved are Human
Respiratory Syncytial Virus (HRSV), Human Metapneumovirus (HMPV), Influenzavirus A and B (FLUA and FLUB), Human
Parainfluenza Virus 1, 2 and 3 (HPIV1, 2 and 3) and Human Rhinovirus (HRV). The purposes of this study were to detect
respiratory viruses in hospitalized children younger than six years and identify the influence of temperature and relative air
humidity on the detected viruses. Samples of nasopharyngeal washes were collected from hospitalized children between
May/2004 and September/2005. Methods of viral detection were RT-PCR, PCR and HRV amplicons were confirmed by
hybridization. Results showed 54% (148/272) of viral positivity....
Tipo: Journal article
Palavras-chave: Viral Respiratory Infections; HRSV; HRV; Temperature; Relative Air Humidity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100011
Essential oil of Lithraea molleoides (Vell.): chemical composition and antimicrobial activity
Provedor de dados: 58
Autores: Shimizu,Mario Tsunezi; Bueno,Leandro de Jesus Franco; Rodrigues,Rosa Fátima Oliveira; Sallowicz,Fabiano Augusto;
Sawaya,Alexandra Christine Helena Frankland; Marques,Márcia Ortiz Maio.
Lithraea molleoides (Vell.) (Anacardiaceae) is a tree found in Brazil, Paraguay, Bolivia, Uruguay, Argentina and Chile. It is
popularly used in the form of alcoholic extracts, decoctions and infusions for the treatment of cough, bronchitis, arthritis, diseases
of the digestive system and as diuretic, tranquilizer, haemostatic and tonic agent. The objectives of this study were the extraction
of the essential oil of the mature fruit, leaves and aerial parts of the plant and quantification of the yield thereof; the identification
and quantification of the principal components of the essential oil and the determination of its antimicrobial activity against
bacteria and yeast. The essential oil yield of the mature fruits was 1.0% but essential oil was not...
Tipo: Journal article
Palavras-chave: Lithraea molleoides; Essential oil; Chemical composition; Antimicrobial activity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400028
Time course of virus-like particles (VLPs) double-stranded rna accumulation in toxigenic and
non-toxigenic strains of Aspergillus flavus
Provedor de dados: 58
Autores: Silva,Valéria N.; Durigon,Edison Luiz; Pires,Maria de Fátima Costa; Lourenço,Alexandre; Faria,Maria Jacinta de;
Corrêa,Benedito.
Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in
order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids
electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was
observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in
toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.
Tipo: Journal article
Palavras-chave: Mycovirus; Double-stranded RNA; Virus-like particles; Aspergillus flavus.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100013
New approach for papaya latex storage without virus degradation
Provedor de dados: 58
Autores: Rodrigues,Silas P.; Andrade,Josemar S.; Ventura,José A.; Fernandes,Patricia M. B.
Papaya meleira virus (PMeV) is the causal agent of papaya (Carica papaya L.) sticky disease, which has been detected through
analysis of its double-stranded RNA (dsRNA) genome from plant latex. In this work we demonstrate that PMeV dsRNA is
protected during 25 days when latex is diluted in citrate buffer pH 5.0 (1:1 v/v) and maintained at -20°C. At the same temperature,
some protection was observed for pure latex or latex diluted in ultra-pure water. Conversely, the dsRNA was almost completely
degraded after 25 days when maintained at 25°C, indicating the need for freezing. The proper procedures to collect and store
papaya latex described here will contribute to efficient and large scale use of molecular diagnosis of PMeV.
Tipo: Journal article
Palavras-chave: Papaya sticky disease; Papaya latex; Papaya meleira virus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100020
Fungicide resistance and genetic variability in plant pathogenic strains of Guignardia citricarpa
Provedor de dados: 58
Autores: Possiede,Y.M.; Gabardo,J.; Kava-Cordeiro,V.; Galli-Terasawa,L.V.; Azevedo,J.L.; Glienke,C.
Citrus black spot (CBS) is a plant disease of worldwide occurrence, affecting crops in Africa, Oceania, and South America. In
Brazil, climate provides favorable conditions and CBS has spread to the Southeast and South regions. CBS is caused by the
fungus Guignardia citricarpa (anamorph: Phyllosticta citricarpa) and its control is based on the use of fungicides, such as
benzimidazoles. In South Africa, the disease was kept under control for 10 years with benomyl, until cases of resistance to high
concentrations of this fungicide were reported from all citrus-producing areas. Azoxystrobin (a strobilurin) has been found
effective in controlling phytopathogens, including CBS, in a wide range of economically important crops. The present study
investigated in...
Tipo: Journal article
Palavras-chave: G. citricarpa; Azoxystrobin; Benomyl; Citrus black spot; Fungicides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200018
Hydroxylation of 1,8-cineole by Mucor ramannianusand Aspergillus niger
Provedor de dados: 58
Autores: Ramos,Aline de Souza; Ribeiro,Joyce Benzaquem; Teixeira,Bruna Gomes; Ferreira,José Luiz Pinto; Silva,Jefferson
Rocha de A.; Ferreira,Alexandre do Amaral; Souza,Rodrigo Octavio Mendonça Alves de; Amaral,Ana Claudia F.
The monoterpenoid 1,8-cineole is obtained from the leaves of Eucalyptus globulus and it has important biological activities. It is a
cheap natural substrate because it is a by-product of the Eucalyptuscultivation for wood and pulp production. In this study, it was
evaluated the potential of three filamentous fungi in the biotransformation of 1,8-cineole. The study was divided in two steps:
first, reactions were carried out with 1,8-cineole at 1 g/L for 24 h; afterwards, reactions were carried out with substrate at 5 g/L for
5 days. The substrate was hydroxylated into 2-exo-hydroxy-1,8-cineole and 3-exo-hydroxy-1,8-cineole by fungi Mucor
ramannianus and Aspergillus niger with high stereoselectivity. Trichoderma harzianum was also tested but no...
Tipo: Journal article
Palavras-chave: Biotransformation monoterpene 1; 8-cineole Mucor ramannianus Aspergillus niger.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100261
Comparative analysis of two component signal transduction systems of the Lactobacillus acidophilus
group
Provedor de dados: 58
Autores: Cui,Yanhua; Qu,Xiaojun.
The Lactobacillus acidophilus group is a phylogenetically distinct group of closely related lactobacilli. Members of this group are
considered to have probiotic properties and occupy different environmental niches. Bacteria generally sense and respond to
environmental changes through two component systems (TCSs) which consist of a histidine protein kinase (HPK) and its cognate
response regulator (RR). With the use of in silico techniques, the five completely sequenced L. acidophilus group genomes were
scanned in order to predict TCSs. Five to nine putative TCSs encoding genes were detected in individual genomes of the L.
acidophilus group. The L. acidophilus group HPKs and RRs were classified into subfamilies using the Grebe and Stock
classification...
Tipo: Journal article
Palavras-chave: Lactobacillus acidophilus group; Two component system; Histidine protein kinase; Response regulator protein;
Bioinformatics analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100019
Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Provedor de dados: 58
Autores: Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia;
Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its
prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead
to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma
synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to
difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of
typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus),
previously...
Tipo: Journal article
Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033
Cytotoxicity of subfractions and compounds from Polymnia sonchifolia
Provedor de dados: 58
Autores: Fernandes,Maria Judite B.; Limas,Caroline; Rossi,Maria Helena; Gonçales,Edlayne; Simoni,Isabela C.
Our previous works showed that extracts and fractions from Polymnia sonchifolia had presented an inhibitory activity on growth
and aflatoxin production by Aspergillus flavus in non-cytotoxic concentrations. In this work, report results on the in vitro
cytotoxicity of subfractions and of a mixture of sesquiterpenes lactones from Polymnia sonchifolia on Vero cells. The cytotoxicity
was assayed through crystal violet staining (CVS) method and the 50% inhibitory concentration for cell growth (IC50) was
calculated. Both subfractions and the mixture of sesquiterpenes lactones did not show in vitro cytotoxicity to Vero cells on active
biologically concentrations against production of aflatoxin B1 by Aspergillus flavus.
Tipo: Journal article
Palavras-chave: Polymnia sonchifolia; Subfractions; Lactones; Cytotoxicity; Crystal violet staining (CVS) method.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400006
Inhibition of listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated
nisin
Provedor de dados: 58
Autores: Malheiros,Patrícia da Silva; Daroit,Daniel Joner; Brandelli,Adriano.
The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was
investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.1 mg/mL and 0.25
mg/mL) and nanovesicle-encapsulated nisin (0.25 mg/mL) were applied onto the surface of cheese samples, and L.
monocytogenes was inoculated before incubation at 6-8°C for 28 days. A bactericidal effect was observed with 0.25 mg/mL free
nisin; a bacteriostatic effect was observed for liposome-encapsulated nisin and 0.1 mg/mL free nisin. Free nisin was more efficient
than nisin-loaded liposomes in controlling L. monocytogenes. Possible reasons for this behavior, and also the significance of nisin
to soft cheeses are...
Tipo: Journal article
Palavras-chave: Nisin; Liposome; Encapsulation; Cheese; Listeria monocytogenes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400024
Bacterioplankton features and its relations with doc characteristics and other limnological variables
in Paraná river floodplain environments (PR/MS-Brazil)
Provedor de dados: 58
Autores: Teixeira,Mariana Carolina; Santana,Natália Fernanda; Azevedo,Júlio César Rodrigues de; Pagioro,Thomaz Aurélio.
Since the introduction of the Microbial Loop concept, many studies aimed to explain the role of bacterioplankton and dissolved
organic carbon (DOC) in aquatic ecosystems. Paraná River floodplain system is a very complex environment where these subjects
were little explored. The aim of this work was to characterize bacterial community in terms of density, biomass and biovolume in
some water bodies of this floodplain and to verify its temporal variation and its relation with some limnological variables,
including some indicators of DOC quality, obtained through Ultraviolet-visible (UV-VIS) and fluorescence spectroscopic
analysis. Bacterial density, biomass and biovolume are similar to those from other freshwater environments and both density and
biomass were...
Tipo: Journal article
Palavras-chave: DOC; Spectroscopy; Bacterioplankton; Floodplain.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300008
Synergistic effects of sulbactam in multi-drug-resistant Acinetobacter baumannii
Provedor de dados: 58
Autores: Temocin,Fatih; Erdinc,Fatma Sebnem; Tulek,Necla; Demirelli,Meryem; Ertem,Gunay; Kinikli,Sami; Koksal,Eda.
Abstract Acinetobacter baumannii is a frequently isolated etiologic agent of nosocomial infections, especially in intensive care
units. With the increase in multi-drug resistance of A. baumannii isolates, finding appropriate treatment alternatives for infections
caused by these bacteria has become more difficult, and available alternate treatments include the use of older antibiotics such as
colistin or a combination of antibiotics. The current study aimed to evaluate the in vitro efficacy of various antibiotic
combinations against multi-drug resistant A. baumannii strains. Thirty multi-drug and carbapenem resistant A. baumannii strains
isolated at the Ankara Training and Research Hospital between June 2011 and June 2012 were used in the study. Antibiotic...
Tipo: Journal article
Palavras-chave: Acinetobacter; Antimicrobial drugs; Drug resistance; Drug synergism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401119
Isolation and characterization of a novel strain of Stenotrophomonas maltophilia possessing various
dioxygenases for monocyclic hydrocarbon degradation
Provedor de dados: 58
Autores: Urszula,Guzik; Izabela,Greń; Danuta,Wojcieszyńska; Sylwia,Łabużek.
A Gram-negative bacterium, designated as strain KB2, was isolated from activated sludge and was found to utilize different
aromatic substrates as sole carbon and energy source. On the basis of morphological and physiochemical characteristics and 16S
rRNA gene sequence analysis, the isolated strain KB2 was identified as Stenotrophomonas maltophilia. Strain KB2 is from among
different Stenotrophomonas maltophilia strains the first one described as exhibiting the activities of three types of dioxygenases
depending on the structure of the inducer. The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase
activity. The activity of 2,3-dioxygenase was detected after phenol induction. Protocatechuate 3,4-dioxygenase was found in crude
cell...
Tipo: Journal article
Palavras-chave: Biodegradation; Stenotrophomonas; Aromatic compounds; Dioxygenases.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200014
Microbiological quality and other characteristics of refrigerated chicken meat in contact with
cellulose acetate-based film incorporated with rosemary essential oil
Provedor de dados: 58
Autores: Melo,Adriane Alexandre Machado de; Geraldine,Robson Maia; Silveira,Miriam Fontes Araujo; Torres,Maria Célia
Lopes; Rezende,Cíntia Silva Minafra e; Fernandes,Thiago Henrique; Oliveira,Antonio Nonato de.
Antimicrobial active packaging delays or inhibits microorganism growth in packed products, and it can be used in a variety of
food systems. The objective of the present research was to develop packaging incorporated with natural antimicrobial agents
(active film). The effects of the active film on the spoilage, pathogenic microorganism counts, pH and color of the refrigerated
chicken breast cuts were analyzed. Cellulose acetate-based active films incorporating two concentrations (20% and 50%, v/w) of
rosemary (Rosmarinus officinalis L.) essential oil were manufactured and placed in contact with the chicken breast cuts for six
days. An analysis of variance and mean comparison tests (Tukey's test, p<0.05) were performed on the results. The films that...
Tipo: Journal article
Palavras-chave: Active packaging; Antimicrobial activity; Cellulose acetate; Rosmarinus officinalis L.; Chicken.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400025
Isolation of filamentous fungi from public telephones of the Metropolitan region of the city of Recife,
PE, Brazil
Provedor de dados: 58
Autores: Coutinho,Flavia Paiva; Cavalcanti,Marilene da Silva; Cordeiro Neto,Francisco.
Fungi can inhabit our organism without causing any harm, but they show themselves when the immunological system is
compromised. In this study, a survey of the occurrence of filamentous fungi was carried out in public telephones of the
Metropolitan Region of the City of Recife, PE, Brazil. This showed the public phones as a possible mean of transmission of fungal
diseases among humans. Samples from the environment, audios, speakers and keyboards were taken at the airport, mall, subway
and bus station in the months of October/2003 (dry season) and June/2004 (rainy season), totaling 120 samples. The procedure of
identification of species was carried out through conventional taxonomy. Thirty-four genera were isolated, totaling 73 species, the
majority...
Tipo: Journal article
Palavras-chave: Filamentous fungi; Mycosis; Public telephones.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200027
Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant
bacteria
Provedor de dados: 58
Autores: Fernandes,Paulo André Vicente; Arruda,Isabel Renata de; Santos,Antônio Fernando Amatto Botelho dos; Araújo,Ana
Albertina de; Maior,Ana Maria Souto; Ximenes,Eulália Azevedo.
Lipopeptides represent a class of microbial surfactants with increasing scientific, therapeutic and biotechnological interests. The
genus Bacillus is a producer of these active compounds, and among them B. subtilis produces surfactin, the most potent
biosurfactant known. These compounds can act as antibiotics, antivirals, antitumorals, immunomodulators and enzyme inhibitors.
In this work, the antimicrobial activity of biosurfactants obtained by cultivation of B. subtilis R14 was investigated against
multidrug-resistant bacteria. During cultivation in defined medium, the surface tension of the medium was reduced from 54 mN/m
in the beginning of the microbial growth to 30 mN/m after 20 hours. A crude surfactant concentration of 2.0 g/L was obtained
after 40...
Tipo: Journal article
Palavras-chave: Biosurfactant; Bacillus subtilis; Lipopeptides; Antimicrobial activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400022
Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in
Brazil
Provedor de dados: 58
Autores: Razzolini,Maria Tereza Pepe; Günther,Wanda Maria Risso; Martone-Rocha,Solange; Luca,Heloísa Duarte de;
Cardoso,Maria Regina Alves.
Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health
concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking
water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells
bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube
Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar
plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical
tests for species...
Tipo: Journal article
Palavras-chave: Aeromonas; Peri-urban area; Water quality.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300020
Stpahylococcus aureus biofilms on central venous haemodialysis catheters
Provedor de dados: 58
Autores: Aoki,Elisabeth Eyko; Pizzolitto,Antonio Carlos; Garcia,Lourdes Botelho; Pizzolitto,Elisabeth Loshchagin.
Biofilm bacterial infections are common in patients undergoing treatment with haemodialysis. This study involved 16 patients (7
males, 9 females; ages from 22 to 81 with an average age of 50) who had had a total of 25 temporary haemodialysis polyurethane
catheter insertions into the subclavian vein (22 dual-lumen and 3 triple-lumen). The catheters remained in place from 3 to 91 days,
on an average of 47 days. The reasons for catheter removal were: bad functioning (44%), suspicion of catheter-related infection
(20%), availability of permanent access (16%), accidental removal (12%), signs and symptoms of infection at the site of catheter
insertion (4%), and exogenous contamination (4%). Positive tip cultures were observed on seven of the catheters (28%),...
Tipo: Journal article
Palavras-chave: Biofilm; Catheter in haemodialysis; Central venous catheter; Catheter-related infections.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400007
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from
Mato Grosso do Sul and analysis of clinical relevance
Provedor de dados: 58
Autores: Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice
Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso.
Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were
identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as
target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium,
3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive.
Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of
these isolates.
Tipo: Journal article
Palavras-chave: Mycobacterium; PCR; Identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013
Antimicrobial susceptibility, hemolysis, and hemagglutination among Aeromonas spp. isolated from
water of a bovine abattoir
Provedor de dados: 58
Autores: Bizani,Delmar; Brandelli,Adriano.
The presence of Aeromonas spp. in food has been demonstrated. They are often introduced from water, which is the natural
habitat of many species and is thought to be the main source of contamination. The occurrence of Aeromonas spp. was
investigated in 70 water samples of a bovine abattoir. Aeromonas spp. were present in 21.4% of water samples. A. hydrophila was
isolated from 11.4% of supplying water samples and from 25.7% of the water drained from carcasses, whereas A. sobria was sole
isolated from 5.7% of supplying water samples. Although greater number of positive samples were from water drained from
carcasses, only A. hydrophila isolates were present. This suggests that the supplying water is the source of contamination. The
antibiotic susceptibility...
Tipo: Journal article
Palavras-chave: Aeromonas; Antibiotic activity; Aquatic environment; Hemolysis; Water.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400016
Acceptable microorganisms concentration in a semen sample for in vitro embryo production
Provedor de dados: 58
Autores: D'Angelo,Magali; Pavão,Danielle L.; Melo,Gisele Martins; Rojas,Natali; Souza,Rosimeire Joaquim; Athayde,Christiani;
Galuppo,Andrea Giannotti; Pinheiro,Eliana Scarcelli.
The aim of this study was to report that the acceptable concentration of microorganisms in a semen sample for insemination may
not be safe for an in vitro fertilization procedure. It seems that the semen sample should be completely germ-free, because of the
excellent microorganism proliferation condition promoted by the in vitro environment.
Tipo: Journal article
Palavras-chave: Semen; Microorganisms; In vitro fertilization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400031
Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea
mays L.)
Provedor de dados: 58
Autores: Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula;
Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and
abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from
Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands.
Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some
high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene
revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains....
Tipo: Journal article
Palavras-chave: Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014
Microbiological aspects of the biofilm on wooden utensils used to make a Brazilian artisanal cheese
Provedor de dados: 58
Autores: Galinari,Éder; Nóbrega,Juliana Escarião da; Andrade,Nélio José de; Ferreira,Célia Lúcia de Luces Fortes.
The artisanal Minas cheese is produced from raw cow's milk and wooden utensils were employed in its manufacture, which were
replaced by other materials at the request of local laws. This substitution caused changes in the traditional characteristics of
cheese. Due to the absence of scientific studies indicating the microbial composition of biofilms formed on wooden forms, tables
and shelves used in these cheese production, the present work evaluated the counts of Staphylococcus aureus, Escherichia coli,
coliforms at 32 °C, yeasts, presumptive mesophilic Lactobacillus spp. and Lactococcus spp. in these biofilms, milk, whey
endogenous culture and ripened cheese in two traditional regions: Serro and Serra da Canastra. Also, we checked for the presence
of...
Tipo: Journal article
Palavras-chave: Biofilm; Artisanal minas cheese; Lactic acid bacteria; Serro; Serra da Canastra.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200047
High occurrence of Fusobacterium nucleatum and Clostridium difficile in the intestinal microbiota of
colorectal carcinoma patients
Provedor de dados: 58
Autores: Fukugaiti,Márcia H.; Ignacio,Aline; Fernandes,Miriam R.; Ribeiro Júnior,Ulysses; Nakano,Viviane;
Avila-Campos,Mario J.
Abstract Colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. Several microorganisms have
been associated with carcinogenesis, including Enterococcus spp., Helicobacter pylori, enterotoxigenic Bacteroides fragilis,
pathogenic E. coli strains and oral Fusobacterium. Here we qualitatively and quantitatively evaluated the presence of oral and
intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. Seventeen patients (between
49 and 70 years-old) visiting the Cancer Institute of the Sao Paulo State were selected, 7 of whom were diagnosed with colorectal
carcinoma. Bacterial detection was performed by qRT-PCR. Although all of the tested bacteria were detected in the majority of
the...
Tipo: Journal article
Palavras-chave: Oral and intestinal bacteria; Colorectal cancer; Intestinal microbiota.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401135
Behavior of Salmonella heidelberg and Salmonella enteritidis strains following broiler chick
inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion
patterns
Provedor de dados: 58
Autores: Borsoi,Anderlise; Santos,Luciana Ruschel do; Rodrigues,Laura Beatriz; Moraes,Hamilton Luiz de Souza; Salle,Carlos
Tadeu Pippi; Nascimento,Vladimir Pinheiro do.
Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry
production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil
since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993.
These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood.
Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of
this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks
(orally...
Tipo: Journal article
Palavras-chave: Salmonella Heidelberg; Salmonella Enteritidis; Fecal Excretion; Villus/crypt ratio; Broiler.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100034
Uptake of free and complexed silver ions by different strains of Rhodotorula mucilaginosa
Provedor de dados: 58
Autores: Gomes,Newton C.M.; Rosa,Carlos A.; Pimentel,Patrícia F.; Mendonça-Hagler,Leda C.S.
Five strains of Rhodotorula mucilaginosa were tested for the ability to accumulate free and complexed silver ions by
metabolism-dependent and -independent processes. The ability to take up Ag+ was observed in both live and dead biomass,
whereas silver dicyanide [Ag (CN)2-] uptake was strictly glucose dependent. In contrast to Ag (CN)2-, glucose addition inhibited
by 16 to 25% the Ag+ uptake rate of living UFMG - Y02, Y27, and Y35 cells, while strains CBS 316 and UFMG-Y01 showed an
improved uptake rate of about 115% and 13%, respectively. The Langmuir sorption model was used to evaluate the silver sorption
capability of the R. mucilaginosa strains. The calculated q max value suggested that R. mucilaginosa strains UFMG-Y27 had the
highest loading capacity....
Tipo: Journal article
Palavras-chave: Bioaccumulation; Biosorption; Rhodotorula mucilaginosa; Silver.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100013
Antagonism of Serratia marcescens towards Phytophthora parasitica and its effects in promoting the
growth of citrus
Provedor de dados: 58
Autores: Queiroz,Brigida Pimentel Villar de; Melo,Itamar Soares de.
Phytophthora parasitica causes serious widespread, and difficult-to-control root rots in warmer regions. This oomycete is one of
the most important pathogen of citrus. This paper reports the biological control of the pathogen by a strain of Serratia marcescens
R-35, isolated from citrus rhizosphere. In greenhouse trials, the bacterium suppressed more than 50% of the disease and promoted
the plant growth.
Tipo: Journal article
Palavras-chave: Biological control; Rhizobacteria; Plant growth promotion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400008
Characterization of pectinase activity for enology from yeasts occurring in Argentine Bonarda grape
Provedor de dados: 58
Autores: Merín,María Gabriela; Martín,María Carolina; Rantsiou,Kalliopi; Cocolin,Luca; de Ambrosini,Vilma Inés Morata.
Pectinolytic enzymes are greatly important in winemaking due to their ability to degrade pectic polymers from grape, contributing
to enhance process efficiency and wine quality. This study aimed to analyze the occurrence of pectinolytic yeasts during
spontaneous fermentation of Argentine Bonarda grape, to select yeasts that produce extracellular pectinases and to characterize
their pectinolytic activity under wine-like conditions. Isolated yeasts were grouped using PCR-DGGE and identified by partial
sequencing of 26S rRNA gene. Isolates comprised 7 genera, with Aureobasidium pullulans as the most predominant pectinolytic
species, followed by Rhodotorula dairenensis and Cryptococcus saitoi. No pectinolytic activity was detected among ascomycetous
yeasts...
Tipo: Journal article
Palavras-chave: Aureobasidium pullulans; Cryptococcus saitoi; Pectinolytic activity; Rhodotorula dairenensis; Winemaking.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300815
Enzymatic resolution of (R,S)-ibuprofen and (R,S)-ketoprofen by microbial lipases from native and
commercial sources
Provedor de dados: 58
Autores: Carvalho,Patrícia de Oliveira; Contesini,Fabiano Jares; Ikegaki,Masaharu.
The enantioselectivity (E) of native lipases from Aspergillus niger, Aspergillus terreus, Fusarium oxysporum, Mucor javanicus,
Penicillium solitum and Rhizopus javanicus in the resolution of (R,S)-ibuprofen and (R,S)-ketoprofenenantiomers by
esterification reaction with 1-propanol in isooctane was compared with known commercial Candida rugosa (Sigma) and Candida
antarctica (Novozym®435) lipases. In the resolution of (R,S)-ibuprofen, C. rugosa lipase showed good selectivity (E = 12) while
Novozym®435 (E = 6.7) and A. niger (E = 4.8) lipases had intermediate selectivities. Other enzymes were much less selective (E
around 2.3 and 1.5), under tested conditions. After preliminary optimization of reaction conditions (water content, enzyme
concentration and...
Tipo: Journal article
Palavras-chave: Lipase (R; S)-ibuprofen (R; S)-ketoprofen enantioselectivity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300024
Degradation of h-acid by free and immobilized cells of Alcaligenes latus
Provedor de dados: 58
Autores: Usha,M.S.; Sanjay,M.K.; Gaddad,S.M.; Shivannavar,C.T.
Alcaligenes latus, isolated from industrial effluent, was able to grow in mineral salts medium with 50 ppm (0.15 mM) of H-acid as
a sole source of carbon. Immobilization of Alcaligenes latus in Ca-alginate and polyurethane foam resulted in cells embedded in
the matrices. When free cells and immobilized cells were used for biodegradation studies at concentration ranging from 100 ppm
(0.3 mM) to 500 ppm (1.15 mM) degradation rate was enhanced with immobilized cells. Cells immobilized in polyurethane foam
showed 100% degradation up to 350 ppm (1.05 mM) and 57% degradation at 500 ppm (1.5 mM). Degradation rate of Ca-alginate
immobilized cells was less as compared to that of polyurethane foam immobilized cells. With Ca-alginate immobilized cells
100%...
Tipo: Journal article
Palavras-chave: H-acid; Biodegradation; Immobilized cells; Alcaligenes latus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400012
Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken
Provedor de dados: 58
Autores: Tamehiro,Claudia Yurika; Alfieri,Alice Fernandes; Médici,Cristina; Alfieri,Amauri Alcindo.
Segmented double-stranded RNA (dsRNA) viruses were identified by polyacrylamide gel electrophoresis (PAGE) technique in
fecal samples from broiler chicken. A total of 378 fecal samples from 1-7 weeks old chickens were analyzed. dsRNA with
migration profile characteristic of avian rotavirus (AvRV), reovirus (ARV) or picobirnavirus (PBV) was identified in 32 (8.5%), 7
(1.8%) and 13 (3.4%) samples, respectively. AvRV and ARV occurred more frequently in chickens up to 1 month old and were
related with enteritis signs. Considering only fecal samples of chickens with diarrhea, the AvRV was detected in 37.8% (14/37)
and the ARV in 13.5% (5/37) of analyzed samples. AvRV was identified in only 1.5% (4/274) and ARV was not detected in
normal feces collected from...
Tipo: Journal article
Palavras-chave: Avian rotavirus; Avian reovirus; Avian picobirnavirus; Broiler chickens; Diarrhea; PAGE.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400013
Biodegradability potential of two experimental landfills in Brazil
Provedor de dados: 58
Autores: Vazoller,Rosana Filomena; Gomes,Luciana Paulo; Baldochi,Viviana Maria Zanta; Villas-Bôas,Denise Maria Fortes;
Badra,Rosileine Jabur; Povinelli,Jurandyr.
Solid wastes anaerobic biodegradability, methane production potential and microbiological composition of two experimental
sanitary landfills in Brazil, running for one year, were evaluated. The two landfills showed a similar organic matter stabilization
during the methane production phase, despite the high heterogeneity of the solid wastes. Both landfills presented the same level of
methane (around 91.5 L CH4 / kg Volatile Total Solids) and organic acids, mainly acetic and butyric acids, in the leachate.
Bacterial isolates belonged to genera Megasphaera, Selenomonas, Methanobacterium, Methanobrevibacter and Methanosarcina.
Tipo: Journal article
Palavras-chave: Solid wastes; Sanitary landfill; Anaerobic biodegradability.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200003
Rabies laboratory diagnosis: peculiar features of samples from equine origin
Provedor de dados: 58
Autores: Peixoto,Zélia M.P.; Cunha,Elenice M. Sequetin; Sacramento,Débora R.V.; Souza,M. Conceição A.M.; Silva,Luzia H.
Queiroz da; Germano,Pedro Leal; Kroeff,Suzana S.; Kotait,Ivanete.
Rabies laboratory diagnosis is performed by using microscopic examination for Negri bodies (MEN), fluorescent-antibody test
(FAT) and mouse inoculation test (MIT). In the majority of cases, when specimens are properly collected and conserved and the
laboratory worker has good experience, agreement among employed techniques is verified. Comparing the sensitivity of these
three diagnosis techniques in 3,713 samples (hippocampus and brain stem) received during 1981-1994 period, being 3,010 from
bovine (983 positives) and 703 from equine (111 positives) species, it was observed that in equine rabid samples, this agreement
was not maintained. For the latter specie, only in few opportunities the Negri bodies could be observed. With respect to FAT, the
test...
Tipo: Journal article
Palavras-chave: Equine rabies; Laboratory diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100017
Candida isolates in tertiary hospitals in northeastern Brazil
Provedor de dados: 58
Autores: Hinrichsen,Sylvia Lemos; Falcão,Érica; Vilella,Tatiana Aguiar Santos; Rêgo,Leandro; Lira,Conceição;
Almeida,Luciano; Martins,Mízia; Araújo,Carmem; Duarte,Marcelo; Lopes,Geraldo.
Candida is an opportunistic pathogen that affects highrisk patients who are either immunocompromised or critically ill and is
associated with almost 80% of all nosocomial fungal infections, representing the major cause of fungemia with high mortality
rates (40%). Candida albicans is the main cause of candidemia and among the non-albicans species C. parapsilosis, C. glabrata
and C. tropicalis are the most frequent agents. The aim of this study was to evaluate the distribution of Candida species in two
tertiary hospitals in Recife, Northeastern Brazil. It began by surveying all positive Candida cultures processed by the
microbiology laboratory from September 2003 to September 2006. The cultures, originated from various types of biological
material (blood,...
Tipo: Journal article
Palavras-chave: Candida; Candidemia; Candida isolates.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200021
A comparative study of coastal and clinical isolates of Pseudomonas aeruginosa
Provedor de dados: 58
Autores: Nair,Anusree V.; Joseph,Neetha; Krishna,Kiran; Sneha,K. G.; Tom,Neenu; Jangid,Kamlesh; Nair,Shanta.
Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium having a versatile metabolic potential and great ecological and
clinical significance. The geographical distribution of P. aeruginosahas revealed the existence of an unbiased genetic arrangement
in terrestrial isolates. In contrast, there are very few reports about P. aeruginosa strains from marine environments. The present
work was aimed at studying the distribution of P. aeruginosa in coastal waters along the Indian Peninsula and understanding the
environmental influence on genotypic, metabolic and phenotypic characteristics by comparing marine and clinical isolates. Of the
785 marine isolates obtained on selective media, only 32 (~4.1%) were identified as P. aeruginosa, based on their fatty...
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Biogeography; FAME profiling; Arbitrarily primed PCR; Environmental distribution.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300725
Tank bromeliad water: similar or distinct environments for research of bacterial bioactives?
Provedor de dados: 58
Autores: Carmo,F.L.; Santos,H.F.; Peixoto,R.S.; Rosado,A.S.; Araujo,F.V.
The Atlantic Rainforest does not have a uniform physiognomy, its relief determines different environmental conditions that define
the composition of its flora and fauna. Within this ecosystem, bromeliads that form tanks with their leaves hold water reservoirs
throughout the year, maintaining complex food chains, based mainly on autotrophic and heterotrophic bacteria. Some works
concluded that the water held by tank bromeliads concentrate the microbial diversity of their ecosystem. To investigate the
bacterial diversity and the potential biotechnology of these ecosystems, tank bromeliads of the Neoregelia cruenta species from
the Atlantic Rainforest in Brazil were used as models for this research. Bacteria isolated from these models were tested for...
Tipo: Journal article
Palavras-chave: Bromeliad; Atlantic Rainforest; Bacteria communities; DGGE.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100024
Integrated control of Penicillium digitatum by the predacious yeast Saccharomycopsis crataegensis
and sodium bicarbonate on oranges
Provedor de dados: 58
Autores: Pimenta,R. S.; Silva,J. F. M.; Coelho,C. M.; Morais,P. B.; Rosa,C. A.; Corrêa Jr,A.
Our investigation of integrated biological control (IBC) started with an assay testing activity of the predacious yeast
Saccharomycopsis crataegensis UFMG-DC19.2 against Penicillium digitatum LCP 4354, a very aggressive fungus that causes
postharvest decay in oranges. Under unfavourable environmental conditions, the yeast showed a high potential for control (39.9%
disease severity reduction) of this fungus. This result was decisive for the next step, in which S. crataegensis was tested in
association with sodium bicarbonate salt, a generally regarded as safe (GRAS) substance. The yeast was able to survive at
different concentrations of the salt (1%, 2% and 5%), and continued to grow for a week at the wound site, remaining viable at high
population for 14...
Tipo: Journal article
Palavras-chave: Biological control; Saccharomycopsis; Penicillium; Postharvest disease; Orange.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200022
Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in
Streptococcus mutans biofilm cells
Provedor de dados: 58
Autores: Tahmourespour,Arezoo; Salehi,Rasoul; Kermanshahi,Rooha Kasra.
Streptococcus mutans (S. mutans), harboring biofilm formation, considered as a main aetiological factor of dental caries. Gtf
genes play an important role in S. mutans biofilm formation. The purpose of this study was to investigate the effect of
Lactobacillus acidophilus-derived biosurfactant on S. mutans biofilm formation and gtfB/C expression level (S. mutans standard
strain ATCC35668 and isolated S. mutans strain (22) from dental plaque). The Lactobacillus acidophilus (L. acidophilus) DSM
20079 was selected as a probiotic strain to produce biosurfactant. The FTIR analysis of its biosurfactant showed that it appears to
have a protein-like component. Due to the release of such biosurfactants, L. acidophilus was able to interfere in the adhesion and...
Tipo: Journal article
Palavras-chave: Biofilm formation; Biosurfactant; GtfB/C; Lactobacillus acidophilus; Real-time RT PCR; Streptococcus mutans.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100042
Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV
Provedor de dados: 58
Autores: Campos,Angélica Cristine de Almeida; Durigon,Edison Luiz; Leal,Andréa Lima; Silva,Tereza Souza; Bosso,Patrícia
Alves Ramos; Moraes,Claudia Trigo Pedroso; Oliveira,Danielle Bruna Leal; Lima,Hildener Nogueira; Vieira,Sandra Elisabete;
Botosso,Viviane Fongaro; Zanotto,Paolo Marinho de Andrade; Stewien,Klaus Eberhard.
The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with
respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against
HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis
of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab
samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University
Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank
sequences. We...
Tipo: Journal article
Palavras-chave: Human Respiratory Syncytial Virus; G Glycoprotein; G2 Region; Phylogenetic Analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300005
Composition, anti-quorum sensing and antimicrobial activity of essential oils from Lippia alba
Provedor de dados: 58
Autores: Olivero-Verbel,Jesus; Barreto-Maya,Ana; Bertel-Sevilla,Angela; Stashenko,Elena E.
Many Gram-negative pathogens have the ability to produce N-acylhomoserine lactones (AHLs) as signal molecules for quorum
sensing (QS). This cell-cell communication system allows them to coordinate gene expression and regulate virulence. Strategies to
inhibit QS are promising for the control of infectious diseases or antibiotic resistant bacterial pathogens. The aim of the present
study was to evaluate the anti-quorum sensing (anti-QS) and antibacterial potential of five essential oils isolated from Lippia alba
on the Tn-5 mutant of Chromobacterium violaceum CV026, and on the growth of the gram-positive bacteria S. aureus ATCC
25923. The anti-QS activity was detected through the inhibition of the QS-controlled violacein pigment production by the sensor...
Tipo: Journal article
Palavras-chave: Violacein; Chromobacterium violaceum; Limonene; Carvone; Geranial; Neral.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300001
Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation
Provedor de dados: 58
Autores: Chellapandi,P.; Jani,Himanshu M.
Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation.
It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic
enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two
isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation
in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum
endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed
by...
Tipo: Journal article
Palavras-chave: Endoglucanase; Streptomyces isolates; Submerged cultivation; Media optimization; Cellulase; CMCase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100026
Characterization and antimicrobial performance of nano silver coatings on leather materials
Provedor de dados: 58
Autores: Lkhagvajav,N.; Koizhaiganova,M.; Yasa,I.; Çelik,E.; Sari,Ö.
In this study, the characterization and the antimicrobial properties of nano silver (nAg) coating on leather were investigated. For
this purpose, turbidity, viscosity and pH of nAg solutions prepared by the sol-gel method were measured. The formation of films
from these solutions was characterized according to temperature by Differential Thermal Analysis-Thermogravimetry (DTA-TG)
equipment. The surface morphology of treated leathers was observed using Scanning Electron Microscopy (SEM). The
antimicrobial performance of nAg coatings on leather materials to the test microorganisms as Escherichia coli, Staphylococcus
aureus, Candida albicans and Aspergillius niger was evaluated by the application of qualitative (Agar overlay method) and
quantitative...
Tipo: Journal article
Palavras-chave: Nano Ag; Antimicrobial activity; Lining leather; Coating.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100041
Microbiological quality of minimally processed vegetables sold in Porto Alegre, Brazil
Provedor de dados: 58
Autores: Silva,Silvia R. Pavan da; Verdin,Sylvia E. Frizzo; Pereira,Dariane C.; Schatkoski,Aline M.; Rott,Marilise B.;
Corção,Gertrudes.
Minimally processed vegetables go through various steps during their preparation, with many modifications to their natural
structure. However, they must maintain the same quality as the fresh produce. The aim of the present study was to quantify
mesophilic and psychrotrophic microorganisms and total and faecal coliforms, and to assess the presence of Escherichia coli,
parasites, and dirt material in ready-to-eat minimally processed vegetables. Fifty-six samples of minimally processed vegetables
were analysed for the presence of mesophilic and psychrotrophic microorganisms by the plate-count method. Monthly means
ranged from 4.7x10(5) to 1.6x10(8) CFU/g and from 7.9x10(6) to 2.7x10(8) CFU/g, respectively for mesophilic and
psychrotrophic microorganisms....
Tipo: Journal article
Palavras-chave: Minimally processed vegetables; Microbiologic quality; Parasitological analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400003
Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from
colombian biodiversity
Provedor de dados: 58
Autores: Niño,Jaime; Narváez,Diana M.; Mosquera,Oscar M.; Correa,Yaned M.
Thirty crude extracts of eight plants belonging to Asteraceae and two to Rubiaceae families collected at different places from the
Regional Natural Park Ucumarí (RNPU), Colombia, were tested for their antibacterial activity against two Gram-positive and two
Gram-negative bacteria and three fungi. Both the antibacterial and the antimycotic activities were tested by the agar well diffusion
method. The cytotoxic activity on the same plant extracts was determined through the brine shrimp lethality bioassay. The extracts
from the Asteraceae family were more bioactive against Bacillus subtilis and Staphylococcus aureus. The extracts of both families
studied were bioactive against the fungi Candida albicans and Fusarium solani. In addition, the extracts of...
Tipo: Journal article
Palavras-chave: Agar well diffusion method; Antimicrobial screening; Artemia salina; Bioprospection; Colombian flora.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400030
Response of Rhodococcus erythropolis strain IBBPo1 to toxic organic solvents
Provedor de dados: 58
Autores: Stancu,Mihaela Marilena.
Abstract Recently, there has been a lot of interest in the utilization of rhodococci in the bioremediation of petroleum contaminated
environments. This study investigates the response of Rhodococcus erythropolis IBBPo1 cells to 1% organic solvents (alkanes,
aromatics). A combination of microbiology, biochemical, and molecular approaches were used to examine cell adaptation
mechanisms likely to be pursued by this strain after 1% organic solvent exposure. R. erythropolis IBBPo1 was found to utilize 1%
alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene) as the sole carbon source.
Modifications in cell viability, cell morphology, membrane permeability, lipid profile, carotenoid pigments profile and 16S rRNA
gene were...
Tipo: Journal article
Palavras-chave: Rhodococcus; Solvents; Morphology; Lipids; Carotenoids.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401009
Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni
in food and faecal samples
Provedor de dados: 58
Autores: Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh.
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of
Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously,
all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples
resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA
extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected
143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and
8,...
Tipo: Journal article
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022
Isolation of Microsporum gypseum in soil samples from different geographical regions of Brazil,
evaluation of the extracellular proteolytic enzymes activities (keratinase and elastase) and molecular
sequencing of selected strains
Provedor de dados: 58
Autores: Giudice,Mauro Cintra; Reis-Menezes,Adriana Araújo; Rittner,Glauce Mary Gomes; Mota,Adolfo José;
Gambale,Walderez.
A survey of Microsporum gypseum was conducted in soil samples in different geographical regions of Brazil. The isolation of
dermatophyte from soil samples was performed by hair baiting technique and the species were identified by morphology studies.
We analyzed 692 soil samples and the recuperating rate was 19.2%. The activities of keratinase and elastase were quantitatively
performed in 138 samples. The sequencing of the ITS region of rDNA was performed in representatives samples. M. gypseum
isolates showed significant quantitative differences in the expression of both keratinase and elastase, but no significant correlation
was observed between these enzymes. The sequencing of the representative samples revealed the presence of two teleomorphic
species of...
Tipo: Journal article
Palavras-chave: Microsporum gypseum; Brazil; Elastase; Keratinase; Sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300007
Antibody responses elicited in mice immunized with Bacillus subtilis vaccine strains expressing Stx2B
subunit of enterohaemorragic Escherichia coli O157:H7
Provedor de dados: 58
Autores: Gomes,P.A.D.P.; Bentancor,L.V.; Paccez,J.D.; Sbrogio-Almeida,M.E.; Palermo,M. S.; Ferreira,R.C.C.; Ferreira,L.C.S.
No effective vaccine or immunotherapy is presently available for patients with the hemolytic uremic syndrome (HUS) induced by
Shiga-like toxin (Stx) producedbyenterohaemorragic Escherichia coli (EHEC) strains, such as those belonging to the O157:H7
serotype. In this work we evaluated the performance of Bacillus subtilis strains, a harmless spore former gram-positive bacterium
species, as a vaccine vehicle for the expression of Stx2B subunit (Stx2B). A recombinant B. subtilis vaccine strain expressing
Stx2B under the control of a stress inducible promoter was delivered to BALB/c mice via oral, nasal or subcutaneous routes using
both vegetative cells and spores. Mice immunized with vegetative cells by the oral route developed low but specific anti-Stx2B...
Tipo: Journal article
Palavras-chave: EHEC; Stx; Bacillus subtilis; Vaccines; HUS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200023
Inoculum padronization for the production of cutinase by Fusarium oxysporum
Provedor de dados: 58
Autores: Pio,Tatiana Fontes; Fraga,Laira Priscila; Macedo,Gabriela Alves.
Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of
this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a
fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium
oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest
activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 x 10(7) spores/mL. The macroscopic
characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results
recorded...
Tipo: Journal article
Palavras-chave: Fusarium oxysporum; Inoculum; Cutinase; Macroscopic characteristics; Fermentation process.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100018
Herbaspirillum seropedicae and sugarcane endophytic interaction investigated by using high pressure
freezing electron microscopy
Provedor de dados: 58
Autores: Silva,Lúcia Gracinda da; Miguens,Flávio Costa; Olivares,Fabio Lopes.
The interaction between sugar cane plantlets and H. seropedicae was investigated using High Pressure Freezing followed by
Freeze Substitution. Microscopical observation showed consistent differences between this approaches when compared with the
conventional preparation, specially related to appearance of the bacteria cell and the endophytic attachment to the host cell wall.
Tipo: Journal article
Palavras-chave: H. seropedicae; High pressure freezing; Endophytic interaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500023
Isolation, Purification and Evaluation of Antibacterial Agents from Aloe vera
Provedor de dados: 58
Autores: Lawrence,Rubina; Tripathi,Priyanka; Jeyakumar,Ebenezer.
The ethanol, methanol and acetone extracts of Aloe vera gel were studied for their antimicrobial activity against four
Gram-positive and Gram-negative bacteria using agar well diffusion method. The extracts showed varied levels of antimicrobial
activity against the tested pathogens. The ethanol and methanol extracts showed higher activity while acetone extract, showed
least or no activity against most of the tested pathogens. Fractions obtained from the extracts by Thin Layer and Column
Chromatography were studied for their antagonistic properties using Spot Assay Technique. Compounds with maximum
antibacterial activity isolated from the ethanol and methanol extracts were identified as p - coumaric acid (Mol. wt.165), ascorbic
acid (Mol. wt.177 ),...
Tipo: Journal article
Palavras-chave: Aloe vera; Gel; Antimicrobial; Compounds.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400023
Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
Provedor de dados: 58
Autores: Machado,Bráulio Caetano; Ferreira,Raquel de Souza; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do
Carmo Sampaio Tavares.
The objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus
(HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and
sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were
identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques
as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription
Polymerase Chain Reaction (RT-PCR) directed to the 5' NTR and VP1 regions of viral genome. The results obtained contributed
with to the...
Tipo: Journal article
Palavras-chave: Enterovirus; Viral meningitis; Molecular and immunological diagnosis; RT-PCR for Enterovirus; Indirect
imunnofluorescense assay.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020
Human papillomavirus infection in women from Tlaxcala, Mexico
Provedor de dados: 58
Autores: Velázquez-Márquez,Noé; Jiménez-Aranda,Lucio Jaime; Sánchez-Alonso,Patricia; Santos-López,Gerardo;
Reyes-Leyva,Julio; Vallejo-Ruiz,Verónica.
Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human
papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV
genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women
from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated
with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute
for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous...
Tipo: Journal article
Palavras-chave: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300027
Performance of an Anaerobic Baffled Reactor (ABR) in treatment of cassava wastewater
Provedor de dados: 58
Autores: Ferraz,Fernanda M.; Bruni,Aline T.; Del Bianchi,Vanildo L.
The performance of an anaerobic baffled reactor (ABR) was evaluated in the treatment of cassava wastewater, a pollutant residue.
An ABR divided in four equal volume compartments (total volume 4L) and operated at 35°C was used in cassava wastewater
treatment. Feed tank chemical oxygen demand (COD) was varied from 2000 to 7000 mg L-1 and it was evaluated the most
appropriated hydraulic retention time (HRT) for the best performance on COD removal. The ABR was evaluated by analysis of
COD (colorimetric method), pH, turbidity, total and volatile solids, alkalinity and acidity. Principal component analysis (PCA)
was carried to better understand data obtained. The system showed buffering ability as acidity decreased along compartments
while alkalinity and pH...
Tipo: Journal article
Palavras-chave: Anaerobic baffled reactor (ABR); Anaerobic digestion; Cassava wastewater; COD removal; Principal
component analysis (PCA).
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100007
Brazilian kefir: structure, microbial communities and chemical composition
Provedor de dados: 58
Autores: Magalhães,Karina Teixeira; Pereira,Gilberto Vinícius de Melo; Campos,Cássia Roberta; Dragone,Giuliano;
Schwan,Rosane Freitas.
Microbial ecology and chemical composition of Brazilian kefir beverage was performed. The microorganisms associated with
Brazilian kefir were investigated using a combination of phenotypic and genotypic methods. A total of 359 microbial isolates
were identified. Lactic acid bacteria (60.5%) were the major isolated group identified, followed by yeasts (30.6%) and acetic acid
bacteria (8.9%). Lactobacillus paracasei (89 isolates), Lactobacillus parabuchneri (41 isolates), Lactobacillus casei (32 isolates),
Lactobacillus kefiri (31 isolates), Lactococcus lactis (24 isolates), Acetobacter lovaniensis (32 isolates), Kluyveromyces lactis (31
isolates), Kazachstania aerobia (23 isolates), Saccharomyces cerevisiae (41 isolates) and Lachancea meyersii (15 isolates)...
Tipo: Journal article
Palavras-chave: Lactobacillus; Saccharomyces; Fermented beverage; Brazilian kefir grains.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200034
Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on
wheat
Provedor de dados: 58
Autores: Oviedo,María Silvina; Sturm,María Elena; Reynoso,María Marta; Chulze,Sofia Noemi; Ramirez,María Laura.
The objectives of this study were to evaluate the ability to produce alternariol (AOH), alternariol monomethyl ether (AME) and
tenuazonic acid (TA) by A. alternata and A. infectoria strains recovered from wheat kernels obtained from one of the main
production area in Argentina; to confirm using AFLPs molecular markers the identify of the isolates up to species level, and to
evaluate the intra and inter-specific genetic diversity of these two Alternaria species. Among all the Alternaria strains tested (254),
84% of them were able to produce mycotoxins. The most frequent profile of toxin production found was the co-production of
AOH and AME in both species tested. TA was only produced by strains of A. alternata. Amplified fragment polymorphism
(AFLPs)...
Tipo: Journal article
Palavras-chave: Alternaria alternata; Alternaria infectoria; Alternariol; Alternariol monomethyl ether; Tenuazonic acid; AFLPs;
Wheat; Genetic variation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200017
Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using
immobilized cells
Provedor de dados: 58
Autores: Orsi,Daniela C.; Kawaguti,Haroldo Y.; Sato,Hélia H.
The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an
alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the
optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved
using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20
g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in
calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration
(25-45%)...
Tipo: Journal article
Palavras-chave: Cell immobilization; Glucosyltransferase; Klebsiella sp; Palatinose; Response surface methodology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100010
Biological control of fire blight in pear orchards with a formulation of Pantoea agglomerans strain Eh
24
Provedor de dados: 58
Autores: Özaktan,Hatice; Bora,Tayyar.
Biological control by using epiphytic bacteria against Erwinia amylovora has been considered as an alternative method for
controlling the disease. Talc-based formulation of Pantoea agglomerans strain Eh-24 was applied at 30% and 100% bloom on two
pear orchards which were selected from different locations in the Aegean Region in Turkey. Pear orchard trials were replicated for
two years (1999 and 2000) in each place. Talc-based formulation of P. agglomerans strain Eh-24 was sprayed on pear trees which
were naturally infected with E. amylovora. In the orchard trials conducted in 1999 and 2000, talc-based formulation of P.
agglomerans strain Eh-24 reduced the percentage of blighted blossoms on pear orchards by 63% to 76%, approximately. Copper...
Tipo: Journal article
Palavras-chave: Biological control; Fire blight; Antagonistic bacteria; Pantoea agglomerans (Erwinia herbicola); Erwinia
amylovora.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200010
Mycoplasmas hyorhinis in different regions of cuba: diagnosis
Provedor de dados: 58
Autores: Lobo,Evelyn; Poveda,Carlos; Suarez,Alejandro; Hernández,Yenney; Ramírez,Ana; Poveda,José B.
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce
pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to
research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with
typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR,
real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M.
hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic...
Tipo: Journal article
Palavras-chave: PCR; Mycoplasma; Diagnostic; Enzootic pneumonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200039
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic
bacteria Streptococcus mutans and Streptococcus sobrinus
Provedor de dados: 58
Autores: Barrientos,Leticia; Herrera,Christian L.; Montenegro,Gloria; Ortega,Ximena; Veloz,Jorge; Alvear,Marysol;
Cuevas,Alejandro; Saavedra,Nicolás; Salazar,Luis A.
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal,
antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical
characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus
mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and
southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were
determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The
minimum...
Tipo: Journal article
Palavras-chave: Propolis; Botanical characterization; Chemical characterization; Streptococcus mutans; Streptococcus sobrinus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038
Erratum - Descriptive study of onychomycosis in a hospital in São Paulo
Provedor de dados: 58
Tipo: Journal article
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401279
Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
Provedor de dados: 58
Autores: Lima,Ilana Schneider; Baumeier,Nicole Carmo; Rosa,Rosimeire Takaki; Campelo,Patrícia Maria Stuelp; Rosa,Edvaldo
Antonio Ribeiro.
This study evaluated the impact of different concentrations of glyphosate (Rondup®) on planktonic and biofilm growth of P.
aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC®15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690
ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their
OD560nm every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in
normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic
cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand,
the herbicide favors a...
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Glyphosate; Growth; Biofilm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300029
Production and properties of an extracellular protease from thermophilic Bacillus sp
Provedor de dados: 58
Autores: Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal.
Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing trisodium citrate reached a
maximum in 9h, with levels of 1.93U/mg protein. The microorganism utilized several carbon sources for the production of
protease. Starch was the best substrate, followed by trisodium citrate, citric acid and sucrose. Among the various organic and
inorganic nitrogen sources, ammonium nitrate was found to be the best. Studies on the protease characterization revealed that the
optimum temperature of this enzyme was 60ºC. The enzyme was stable for 2h at 30ºC, while at 40ºC and 80ºC, 14% and 84% of
the original activities were lost, respectively. The optimum pH of the enzyme was found to be 8.0. After incubation of crude
enzyme...
Tipo: Journal article
Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100015
Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive
patients with chronic gingivitis and necrotizing periodontitis
Provedor de dados: 58
Autores: Gaetti-Jardim Júnior,Elerson; Nakano,Viviane; Wahasugui,Thais C.; Cabral,Fátima C.; Gamba,Rosa;
Avila-Campos,Mario Julio.
The purpose of this study was to determine the prevalence of enteric bacteria and yeasts in biofilm of 80 HIV-positive patients
with plaque-associated gingivitis or necrotizing periodontitis. Patients were subjected to extra, intra oral and radiographic
examinations. The oral hygiene, bleeding on probing, gingival conditions, and attachment loss were evaluated. Clinical specimens
were collected from gingival crevices or periodontal pockets, transferred to VMGA III, diluted and transferred to Sabouraud
Dextrose agar with 100 µg/ml of chloramphenicol, peptone water, EVA broth, EMB agar, SS agar, Bile esculin agar and Brilliant
green agar. Isolation of yeasts was carried out at room temperature, for 3-7 days; and for the isolation of enteric microorganisms...
Tipo: Journal article
Palavras-chave: HIV patients; Candida albicans; Enterococci; Enterobacteria.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200011
Selection of 5-fluorocytosine-resistant mutants from an Aspergillus niger citric acid-producing strain
Provedor de dados: 58
Autores: Conte,Ana Paula de F.; Marin,José M.
Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to
5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of
protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with
improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental
strain.
Tipo: Journal article
Palavras-chave: Aspergillus niger; Citric acid; 5-fluorocytosine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100001
A method to estimate the biomass of Spirulina platensis cultivated on a solid medium
Provedor de dados: 58
Autores: Pelizer,Lúcia Helena; Moraes,Iracema de Oliveira.
This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a
support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in
semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous
cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins,
pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and
optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane
bagasse...
Tipo: Journal article
Palavras-chave: Spirulina platensis; Solid state cultivation; Estimative of biomass; Sugarcane bagasse; Protein content.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300024
Heavy metal resistance of microorganisms isolated from coal mining environments of Santa Catarina
Provedor de dados: 58
Autores: Castro-Silva,Marcus Adonai; Lima,André Oliveira de Souza; Gerchenski,Ana Valéria; Jaques,Daniela Batista;
Rodrigues,André Luis; Souza,Pricila Lima de; Rörig,Leonardo Rubi.
The coal mining activity is characterized by the generation of large amount of by-products. One of them is pyrite, which tends to
acidify the water, solubilizing heavy metals. As a consequence the environment becomes acid and rich in heavy metals, selecting
microorganisms able to survive in this condition, which are of great interest as bioremediation agents. This work describes the
isolation and characterization of microorganisms from a coal mining area in Santa Catarina. These microorganisms comprised
bacteria, fungi and yeasts resistant to zinc, nickel and cadmium.
Tipo: Journal article
Palavras-chave: Heavy metal resistance; Coal mining; Acidophilic microorganisms; Bioremediation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500015
Evaluation of the Diramic system for urine cultures
Provedor de dados: 58
Autores: Higuchi,Cristian Kendi; Corrêa,Carlos Roberto; Rall,Vera Lucia Mores; Fernandes Junior,Ary.
The use of the Diramic system in microbiological diagnosis of urinary tract infections (UTI) was evaluated. This system was
developed at the National Center for Scientific Research of Cuba, and it reads turbidimetric changes of microbial growth in
culture media at 37ºC incubated for 4 hours. A total of 396 urine specimens were tested in the Laboratory of Microbiology of the
School of Medicine - UNESP, Brazil using the Diramic system and the counting of colony forming units per urine millimeter
(calibrated loop) as the reference method. The coincidence rate between the two methods was 96.46% (382 urine samples), and
the differences in results were not significant (p<0.05). Sensitivity and specificity rates were 84.37% and 98.80%, respectively.
False...
Tipo: Journal article
Palavras-chave: Diramic; Calibrated loop; Urinary tract infection; Urine cultures.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300005
Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
Provedor de dados: 58
Autores: Silva,Daiani M.; Batista,Luís R.; Rezende,Elisângela F.; Fungaro,Maria Helena P.; Sartori,Daniele; Alves,Eduardo.
In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have
been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section
from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and
sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A.
carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger.
The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp
species,...
Tipo: Journal article
Palavras-chave: Polyphasic Taxonomy β -tubulin gene Aspergillus spp morphology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200044
Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH
indicator
Provedor de dados: 58
Autores: Lisboa,Helen Cristina Fávero; Biasetto,Carolina Rabal; Medeiros,João Batista de; Araújo,Ângela Regina; Silva,Dulce
Helena Siqueira; Teles,Helder Lopes; Trevisan,Henrique Celso.
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for
development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of
Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project
"Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The
compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by
extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the
reduction of the pH increases...
Tipo: Journal article
Palavras-chave: Esterase; PH indicator; Enzyme activity; Endophytic fungi.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300042
Work of adhesion of dairy products on stainless steel surface
Provedor de dados: 58
Autores: Bernardes,Patrícia Campos; Araújo,Emiliane Andrade; Pires,Ana Clarissa dos Santos; Fialho Júnior,José Felício
Queiroz; Lelis,Carini Aparecida; Andrade,Nélio José de.
The adhesion of the solids presents in food can difficult the process of surface cleaning and promotes the bacterial adhesion
process and can trigger health problems. In our study, we used UHT whole milk, chocolate based milk and infant formula to
evaluate the adhesion of Enterobacter sakazakii on stainless steel coupons, and we determine the work of adhesion by measuring
the contact angle as well as measured the interfacial tension of the samples. Inaddition we evaluated the hydrophobicity of
stainless steel after pre-conditioning with milk samples mentioned. E. sakazakii was able to adhere to stainless steel in large
numbers in the presence of dairy products. The chocolate based milk obtained the lower contact angle with stainless steel surface,
higher...
Tipo: Journal article
Palavras-chave: Enterobacter sakazakii; Hydrophobicity; Adhesion work; Interfacial tension; Dairy products.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400004
Production of fungal chitosan in liquid cultivation using apple pomace as substrate
Provedor de dados: 58
Autores: Streit,Fernanda; Koch,Fernanda; Laranjeira,Mauro C.M.; Ninow,Jorge L.
In this work, we propose the reuse of apple pomace as a substrate for fungal chitosan production by liquid cultivation of
Gongronella butleri CCT4274. Different concentrations of reducing sugars and sodium nitrate were added to the aqueous extract
of apple pomace and the best result was obtained with 40 g/L of reducing sugars and 2.5 g/L of sodium nitrate. The results
indicate the possibility of producing 1.19 g/L of chitosan per liter of culture medium after 72.5 hours of cultivation, representing
around 21% of the biomass content.
Tipo: Journal article
Palavras-chave: Apple pomace; Fungal chitosan; Gongronella butleri; Liquid cultivation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100003
Biosurfactant production by Pseudomonas aeruginosain kefir and fish meal
Provedor de dados: 58
Autores: Kaskatepe,Banu; Yildiz,Sulhiye; Gumustas,Mehmet; Ozkan,Sibel A.
The aim of this study was to increase rhamnolipid production by formulating media using kefir and fish meal for Pseudomonas
aeruginosa strains isolated from different environmental resources. The strains, named as H1, SY1, and ST1, capable of
rhamnolipid production were isolated from soil contaminated with wastes originating from olive and fish oil factories.
Additionally, P. aeruginosa ATCC 9027 strain, which is known as rhamnolipid producer, was included in the study. Initially,
rhamnolipid production by the strains was determined in Mineral Salt Medium (MSM) and then in media prepared by using kefir
and fish meal. The obtained rhamnolipids were purified and quantified according to Dubois et al. (1956). The quantity of
rhamnolipids of ATCC, H1 and SY1...
Tipo: Journal article
Palavras-chave: Biosurfactant; Pseudomonas aeruginosa; Rhamnolipid; Kefir; Fish meal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300855
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry
Provedor de dados: 58
Autores: Rocha,Ticiana Silva; Baptista,Ana Angelita Sampaio; Donato,Tais Cremasco; Milbradt,Elisane Lenita;
Okamoto,Adriano Sakai; Andreatti Filho,Raphael Lucio.
In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in
improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an
important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its
beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of
Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its
adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and
cecum were...
Tipo: Journal article
Palavras-chave: Lactobacillus; Identification; Intestinal adhesion.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive
Care Units patients
Provedor de dados: 58
Autores: Silva,Rosana Bellan de Oliveira; Fusco-Almeida,Ana Marisa; Matsumoto,Marcelo Teruyuki; Baeza,Lilian Cristiane;
Benaducci,Tatiane; Mendes-Giannini,Maria José Soares.
Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this
study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified
by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole,
5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of
the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found.
Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The
dendogram analysis...
Tipo: Journal article
Palavras-chave: Trichosporon; PCR; RAPD; Susceptibility; Antifungal drugs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033
Treatment of serum with supernatants from cultures of Candida albicans reduces its
serum-dependent phagocytosis
Provedor de dados: 58
Autores: Santos,Aderbal Antonio dos; Sá,Eglius Alexandre Colignese de; Gaziri,Luis Carlos Jabur; Felipe,Ionice.
Candida albicans is a potent activator of the complement system, and heat labile opsonins produced by activation of C3 (C3b and
iC3b) enhance phagocytosis of C. albicans mediated by complement receptors. In this study we treated mouse serum with
supernatants from cultures of a protease producer strain of C. albicans and evaluated the ability of this serum to enhance
phagocytosis of C. albicans. Cell-free supernatants from cultures of C. albicans were concentrated 5 fold and added to mouse
serum for 30 min at 37ºC, before using this serum for opsonization of glutaraldehyde-fixed yeast cells. We observed that normal
mouse serum increased about 3 fold the phagocytosis of C. albicans by mice peritoneal macrophages, whereas supernatant-treated
serum did not...
Tipo: Journal article
Palavras-chave: Candida albicans; Phagocytosis; Proteinase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100016
Antifungal properties of clove oil (Eugenia caryophylata) in sugar solution
Provedor de dados: 58
Autores: Núñez,Lidia; D'Aquino,Miguel; Chirife,Jorge.
The effect of temperature, concentration and contact time on the fungicidal effect of clove oleoresin dispersed in a concentrated
sugar solution at 21 and 37ºC, and clove oleoresin at 0.2 to 0.8% (v/v) was studied. The test microorganisms were Candida
albicans, Penicillium citrinum, Aspergillus niger and Trichophyton mentagrophytes. The fungicidal effect was enhanced at 37ºC;
at this temperature short contact times (e.g. 1 min.) were enough to eliminate a microbial inoculum of 10(6) c.f.u./ml of C.
albicans. Although clove oleoresin caused important lethal effect, P. citrinum and A. niger were more resistant. After 60 minutes,
clove oleoresin dispersed (0.4% v/v) in concentrated sugar solution caused a 99.6% reduction of the initial population (10(6)...
Tipo: Journal article
Palavras-chave: Clove oil; Fungicide; Disinfectant; Sugar.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200010
Trichosporon species isolated from the perigenital region, urine and catheters of a Brazilian
population
Provedor de dados: 58
Autores: Silvestre Junior,Agenor Messias; Miranda,Mauricio Alexandre Bandeira Rampazzo; Camargo,Zoilo Pires de.
The present study aimed to 1) determine the colonization rates of medically important Trichosporon species on normal perigenital
skin and 2) determine the isolation rates of Trichosporon spp. isolated from the urine and catheters of Brazilian patients
hospitalized in the Intensive Care Unit (ICU). The overall colonization rate of Trichosporon spp. was 11.15% (112 isolates). The
most common species isolated from normal perigenital skin was T. cutaneum (29.46%), followed by T. asteroides (20.53%), T.
ovoides (15.17%), T. inkin (10.71%), T. mucoides (8.92%), and T. asahii (6.25%). From urine and catheters, T. asahii was the
species most commonly isolated (76.5%; n =23), followed by T. inkin (16.6%; n = 5) and T. asteroides (6.6%; n = 2). In addition,
the...
Tipo: Journal article
Palavras-chave: Trichosporon spp.; Epidemiology; Perigenital skin; Superficial mycosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300013
Mechanism of bactericidal activity of Silver Nitrate - a concentration dependent bi-functional
molecule
Provedor de dados: 58
Autores: Pandian,Sureshbabu Ram Kumar; Deepak,Venkataraman; Kalishwaralal,Kalimuthu; Viswanathan,Pushpa;
Gurunathan,Sangiliyandi.
Silver nitrate imparts different functions on bacteria depending upon its concentration. At lower concentration it induced synthesis
of nanoparticles, whereas at higher concentrations it induced cell death. Bacillus licheniformis was used as model system. The
MIC was 5 mM, and it induced catalase production, apoptotic body formation and DNA fragmentation.
Tipo: Journal article
Palavras-chave: Silver nitrate; Bacillus licheniformis; Nanoparticle synthesis; Apoptosis; DNA fragmentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300033
Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters
studies and application tools
Provedor de dados: 58
Autores: Silva,Tatyane R.; Valdman,Erika; Valdman,Belkis; Leite,Selma G.F.
The optimal conditions for salicylic acid biodegradation by Pseudomonas fluorescens HK44 were determined in this study with
the intention to create a microbial sensor. Kinetic experiments permitted a definition of 60 and 30min the time needed to achieve
the maximum degradation of salicylic acid presented in a medium with and without yeast extract, respectively. The degradation in
medium without yeast extract and the quantification by spectrophotometry 230 nm were selected to be used in further tests. The
use of preactivated cells or on the exponential growth phase showed better salicylic acid degradation percentages when compared
to nonactivated cells or on the stationary growth state. Finally, the best cellular concentration used on the salicylic acid...
Tipo: Journal article
Palavras-chave: Salicylic acid; Pseudomonas fluorescens HK44; Bioremediation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100009
Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate
Provedor de dados: 58
Autores: Accorsini,Fábio Raphael; Mutton,Márcia Justino Rossini; Lemos,Eliana Gertrudes Macedo; Benincasa,Maria.
Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is
given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its
use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is
recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their
capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all
showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica,...
Tipo: Journal article
Palavras-chave: Yeast; Biosurfactants; Glycerol; Soybean oil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100013
Whole cells in enantioselective reduction of benzyl acetoacetate
Provedor de dados: 58
Autores: Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da;
Souza,Rodrigo Octavio Mendonça Alves de.
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven
microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and
85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2
cycles of reaction.
Tipo: Journal article
Palavras-chave: Biotransformation chiral reduction β -ketoester Kluyveromyces marxianus immobilization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300023
'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged
fermentation
Provedor de dados: 58
Autores: Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Di
Luccio,Marco; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen.
The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions.
In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of
a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the
results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization
by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0,
6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold...
Tipo: Journal article
Palavras-chave: Sporidiobolus pararoseus; Experimental design; 'synthetic lipase'; Submerged fermentation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400033
Efficacy of fungal decolorization of a mixture of dyes belonging to different classes
Provedor de dados: 58
Autores: Przystas,Wioletta; Zablocka-Godlewska,Ewa; Grabinska-Sota,Elzbieta.
Dyes are the most difficult constituents to remove by conventional biological wastewater treatment. Colored wastewater is mainly
eliminated by physical and chemical procedures, which are very expensive and have drawbacks. Therefore, the advantage of using
biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less
toxic products. To prove the possibility of using fungal processes for decolorization and other applications, the analysis of the
toxicity of the processes' products is required. The decolorization of the mixture of two dyes from different classes triphenylmethane brilliant green and azo Evans blue (GB - total concentration 0.08 g/L, proportion 1:1 w/w) - by Pleurotus...
Tipo: Journal article
Palavras-chave: Decolorization; Fungal dye removal; Dye mixture; Zootoxicity; Phytotoxicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200415
Microbial populations of crude oil spill polluted soils at the Jordan-Iraq desert (the Badia region)
Provedor de dados: 58
Autores: Saadoun,Ismail; Mohammad,Munir J.; Hameed,Khalid M.; Shawaqfah,Mo'ayyad.
Microbial populations' inhabitants in crude petroleum contaminated soils were analyzed in relation with the soil characteristics. A
noticeable greater decline of bacterial counts and diversity but a prevalence of the genus Pseudomonas over the other identified
genera in the fresh contaminated soils as compared to the old ones was observed.
Tipo: Journal article
Palavras-chave: Bacteria; Crude oil; Fungi; Soil; Streptomycetes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300008
Activity of the aqueous extract from Polymnia sonchifolia leaves on growth and production of
aflatoxin B1 by Aspergillus flavus
Provedor de dados: 58
Autores: Pinto,Marina M.; Gonçalez,Edlayne; Rossi,Maria H.; Felício,Joana D.; Medina,Cláudia S.; Fernandes,Maria J.B.;
Simoni,Isabela C.
The aqueous extract from Polymnia sonchifolia leaves (AE) was tested for inhibitory activity on aflatoxin B1(AFB1) production
and growth of Aspergillus flavus. The cytotoxicity of AE on Vero cells was also performed. Suspensions of A. flavus spores were
inoculated into 50 mL of YES medium together with different concentrations of the AE. The aflatoxin B1 was extracted, analyzed
by thin layer chromatography and quantified by photodensitometry. All the concentrations of AE induced inhibition of AFB1
production. The aqueous extract showed in vitro cytotoxicity to Vero cells only at concentrations above 500 µg/mL.
Tipo: Journal article
Palavras-chave: Aflatoxin B1; Aspergillus flavus; Polymnia sonchifolia; Cytotoxicity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200011
Detection of airborne psychrotrophic bacteria and fungi in food storage refrigerators
Provedor de dados: 58
Autores: Altunatmaz,Sema Sandikci; Issa,Ghassan; Aydin,Ali.
The purpose of this study was to determine the microbiological air quality (psychrotrophic bacteria and airborne fungi) and
distribution of fungi in different types of ready-to-eat (RTE) food-storage refrigerators (n=48) at selected retail stores in the city of
Edirne, Turkey. Refrigerators were categorized according to the type of RTE food-storage: meat products, vegetables, desserts, or
a mix of food types. Microbiological quality of air samples was evaluated by using a Mas-100 Eco Air Sampler. Four refrigerators
(all containing meat products, 8.3%) produced air samples with undetectable microorganisms. The highest detected mean value of
airborne psychrotrophic bacteria and fungi was 82.3 CFU/m³ and 54.6 CFU/m³, respectively and were found in mixed-food...
Tipo: Journal article
Palavras-chave: Airborne fungi; Psychrotrophic bacteria; Ready-to-eat food; Air sampler; Refrigerator.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400027
Isolation of Leptospira santarosai, serovar guaricura from buffaloes (Bubalus bubalis) in Vale do
Ribeira, São Paulo, Brazil
Provedor de dados: 58
Autores: Vasconcellos,Silvio A.; Oliveira,João C.F.; Morais,Zenáide M.; Baruselli,Pietro S.; Amaral,Renato; Pinheiro,Sônia R.;
Ferreira,Fernando; Ferreira-Neto,José S.; Schönberg,Arno; Hartskeerl,Rudy A.
In April 1998 urine samples from adult female buffaloes were collected in a farm located in Registro, Vale do Ribeira, São Paulo
State, Brazil. The urine samples obtained after furosemide injection were immediately transported to the laboratory in liquid
modified EMJH medium and seeded, by the serial dilution technique, into Fletcher's or modified EMJH-0.2% agar, both of them
with 5-fluorouracil 100mg/mL. The intraperitoneoum inoculation of 0.5 mL was also performed with each urine sample in young,
adult hamsters (Mesocricetus auratus). All samples seeded directly in culture medium were contaminated. The hamsters did not
show any sign of disease and were killed at the 21st post inoculation day. At this time kidney cultures of these animals were
performed...
Tipo: Journal article
Palavras-chave: Leptospirosis; Serogroup Sejroe; Serovar guaricura; Buffalo.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400008
Shiitake (lentinula edodes) production on a sterilized bagasse substrate enriched with rice bran and
sugarcane molasses
Provedor de dados: 58
Autores: Rossi,Ivan Henrique; Monteiro,Antonio Carlos; Machado,José Octavio; Andrioli,João Luciano; Barbosa,José Carlos.
This investigation was performed to evaluate the biological efficiency (BE), mean mushroom weight (MMW), mean number of
mushroom (MNM) and mushroom quality of Shiitake [ Lentinula edodes (Berk.) Pegler] when grown on a sterilized substrate
composed by sugarcane bagasse enriched with rice bran and sugarcane molasses. The proportions of rice bran were 0, 15, 20, 25
and 30% (dry weight/dry weight of bagasse); and the concentrations of sugarcane molasses were 0, 30 and 60 g/kg (dry
weight/dry weight of bagasse plus rice bran). Four flushes were obtained during the production cycle, providing 3 accumulated
productions which were used for production analysis. The substrate supplemented with 25 and 30% rice bran yielded the highest
BE (98.42 and 99.84%,...
Tipo: Journal article
Palavras-chave: Lentinula edodes; Shiitake; Sugarcane bagasse.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100014
CULTIVATION OF THE EDIBLE MUSHROOM OUDEMANSIELLA CANARII (JUNGH.)
HÖHN. IN LIGNOCELLULOSIC SUBSTRATES
Provedor de dados: 58
Autores: Ruegger,Marcelo José Silveira; Tornisielo,Sâmia Maria Tauk; Bononi,Vera Lúcia Ramos; Capelari,Marina.
The edible mushroom Oudemansiella canarii (Jungh.) Höhn is common in the Brazilian territory, being found in different biomas,
where they colonize several plant species. In this study, the O. canarii cultivation was evaluated in polypropilene bags containing
sugar-cane bagasse (200 g) or eucalyptus sawdust (200 g) supplemented with wheat bran (50 g). The composts were sterilized at
121ºC for 1 hour, after cooling they were inoculated with 3 g of spawn and then remained incubated at 25ºC until the basidiomata
primordia formation. The mushrooms, harvested after the pilei opening, presented varied sizes reaching 9 cm of diameter and 10
cm of height. The fresh mushrooms presented mild taste and soft consistency. When kept at 4ºC, they maintained good...
Tipo: Journal article
Palavras-chave: Oudemansiella canarii; Basidiomycetes; Mushroom; Solid substrates; Biodegradation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300009
Synergistic growth effect among bacteria recovered from root canal infections
Provedor de dados: 58
Autores: Moreira Júnior,Gil; Ribeiro Sobrinho,Antônio Paulino; Bambirra,Bernardo Henrique Silva; Bambirra,Felipe Henrique
Silva; Carvalho,Maria Auxiliadora Roque; Farias,Luiz Macedo; Nicoli,Jacques Robert; Moreira,Elizabeth Spangler.
The objective of this study was to determine the ecological relationships between bacterial species that colonize infected root
canals. Root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and
antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using
the double layer diffusion method. Peptostreptococcus prevotii triggered a significant increase of Fusobacterium nucleatum
growth, while the former bacteria did not affect the growth of P. prevotii. The bacterial species did not produce antagonism
activity against itself or against any of the other two species. Despite many studies have demonstrated the capability of root
canal...
Tipo: Journal article
Palavras-chave: Root canal; Infection; Bacterial relationships.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300017
Nasopharyngeal Gram-negative bacilli colonization in Brazilian children attending day-care centers
Provedor de dados: 58
Autores: Lima,Ana Beatriz Mori; Leão,Lara Stefânia Netto de Oliveira; Oliveira,Luciana Silva da Cruz; Pimenta,Fabiana
Cristina.
This study aimed at determining prevalence and resistance profile of Gram-negative bacilli isolated from nasopharynx of children
attending day-care centers in Goiânia (Brazil). P. aeruginosa (100.0%), E. coli (50.0%), K. pneumoniae (35.3%), and E. aerogenes
(16.7%) were the most frequent multi-drug resistant microorganisms isolated. No production of ESBL was detected.
Tipo: Journal article
Palavras-chave: Gram-negative bacilli; Nasopharyngeal colonization; Infants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100005
Characterization of the virulence, growth temperature and antibiotic resistance of the Campylobacter
jejuni IAL 2383 strain isolated from humans
Provedor de dados: 58
Autores: Fonseca,B.B.; Rossi,D.A.; Maia,C.A.; Nalevaiko,P.C.; Melo,R.T.; Cuccato,L.P.; Beletti,M.E.
The objective of this study was to characterize the C. jejuni IAL2383 strain isolated from humans in Brazil. Transcripts for the
racR, dnaJ and ciaB genes were found and flaA, plda and cadF genes were present in the genome and bacteria was sensitive to
most of the important antimicrobials used to treat humans. C. jejuni IAL2383 is a good experimental model to analyze the
interactions with cells.
Tipo: Journal article
Palavras-chave: Virulence; Experimental model; Thermotolerance; Campylobacter jejuni.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100039
Antimicrobial and toxicological studies of some metal complexes of
4-methylpiperazine-1-carbodithioate and phenanthroline mixed ligands
Provedor de dados: 58
Autores: Kalia,S.B.; Kaushal,G.; Kumar,M.; Cameotra,S.S.; Sharma,A.; Verma,M.L.; Kanwar,S.S.
A few mixed ligand transition metal carbodithioate complexes of the general formula [M(4-MPipzcdt)x(phen)y]Y (M = Mn(II),
Co(II), Zn(II); 4-MPipzcdt = 4-methylpiperazine-1-carbodithioate; phen = 1,10-phenanthroline; x = 1 and y = 2 when Y = Cl; x =
2 and y = 1 when Y = nil) were synthesized and screened for their antimicrobial activity against Candida albicans, Escherichia
coli, Pseudomonas aeruginosa,Staphylococcus aureus andEnterococcusfaecalis by disk diffusion method. All the complexes
exhibited prominent antimicrobial activity against tested pathogenic strains with the MIC values in the range <8-512
μgmL-1. The complexes [Mn(4-MPipzcdt)2(phen)] and [Co(4-MPipzcdt)(phen)2]Cl inhibited the growth of Candida
albicans at a concentration as low...
Tipo: Journal article
Palavras-chave: Antimicrobial; Metal ions; 4-methylpiperazine-1-carbodithioate; MIC; Zone of inhibition.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400024
An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium
fortuitum
Provedor de dados: 58
Autores: Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula;
Lilenbaum,Walter.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M.
fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an
alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of
or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a
preadsorption antigen.
Tipo: Journal article
Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019
Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between
Penicillium expansum and Penicillium griseoroseum
Provedor de dados: 58
Autores: Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de;
Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de.
Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P.
expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a
prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were
isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic
did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented
enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P.
griseoroseum...
Tipo: Journal article
Palavras-chave: Protoplast fusion; Pectinases; Penicillium.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine
in southern of Brazil
Provedor de dados: 58
Autores: Costa,Mateus Matiuzzi da; Drescher,Guilherme; Maboni,Franciele; Weber,Shana; Botton,Sônia de Avila;
Vainstein,Marilene Henning; Schrank,Irene Silveira; Vargas,Agueda Castagna de.
The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of
Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC,
STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of
virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4
(4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%),
erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin
(58.53%),...
Tipo: Journal article
Palavras-chave: Plasmids; Virulence factors; Antimicrobial resistance; Swine.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400027
Evaluation of antibacterial properties of Barium Zirconate Titanate (BZT) nanoparticle
Provedor de dados: 58
Autores: Mohseni,Simin; Aghayan,Mahdi; Ghorani-Azam,Adel; Behdani,Mohammad; Asoodeh,Ahmad.
So far, the antibacterial activity of some organic and inorganic compounds has been studied. Barium zirconate titanate
[Ba(Zr xTi1-x)O3] (x = 0.05) nanoparticle is an example of inorganic materials. In vitro studies have
provided evidence for the antibacterial activity of this nanoparticle. In the current study, the nano-powder was synthesized by
sol-gel method. X-ray diffraction showed that the powder was single-phase and had a perovskite structure at the calcination
temperature of 1000 ºC. Antibacterial activity of the desired nanoparticle was assessed on two gram-positive
(Staphylococcus aureus PTCC1431 and Micrococcus luteus PTCC1625) and two gram-negative (Escherichia coli HP101BA
7601c and clinically isolated Klebsiella...
Tipo: Journal article
Palavras-chave: Nanoparticles; Antibiotics; Barium zirconate titanate; Ceramics; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400033
Production and properties of alpha-amylase from thermophilic Bacillus sp.
Provedor de dados: 58
Autores: Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara.
alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) production by thermophilic Bacillus sp strain SMIA-2
cultivated in liquid media containing soluble starch reached a maximum at 48h, with levels of 57U/mL. Studies on the a-amylase
characterization revealed that the optimum temperature for activity was 70ºC. The enzyme was stable for 2h at 50ºC, while at
60ºC, 70ºC and 90ºC, 4%, 13% and 38% of the original activities were lost, respectively. The optimum pH of the enzyme was 7.5.
After incubation of crude enzyme solution for 24h at pH 7.5, a decrease of about 5% of its original activity was observed. The
enzyme was strongly inhibited by Co2+, Cu2+ and Ba2+, but less affected by Ca2+, Mg2+, Ni2+, Sr2+ and Mn2+. The enzyme in
1M and 5M NaCl...
Tipo: Journal article
Palavras-chave: Alpha-amylase; Thermophilic bacterium; Bacillus sp.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100012
Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid
production
Provedor de dados: 58
Autores: Oliveira,Adriano H.; Ogrodowski,Cristiane C.; Macedo,André C. de; Santana,Maria Helena A.; Gonçalves,Luciana R.B.
In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion
medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch
fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented
with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and
hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration
or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results
suggest the...
Tipo: Journal article
Palavras-chave: Hyaluronic acid; Cashew apple juice; Submerged fermentation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400011
Technological application of an extracellular cell lytic enzyme in xanthan gum clarification
Provedor de dados: 58
Autores: Shastry,Suresh; Prasad,M.S.
An extracellular cell lytic enzyme from Pseudomonas sp. was active on heat killed cells of Xanthomonas campestris. The lytic
activity caused enzymatic digestion of X.campestris xanthan gum. Digestion was effective for highly viscous native xanthan 2.0%
(w/v) and 2.5% (w/v) commercial Sigma xanthan. Scanning electron microscopy and SDS-PAGE observations confirmed the cell
lytic action on X.campestris cells.
Tipo: Journal article
Palavras-chave: Xanthan; Cell lytic enzyme.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100012
Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection
Provedor de dados: 58
Autores: Lima-Neto,Reginaldo; Santos,Cledir; Lima,Nelson; Sampaio,Paula; Pais,Célia; Neves,Rejane P.
Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by
supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have
been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the
routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical
phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture
collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical...
Tipo: Journal article
Palavras-chave: BRCs; Candida; Clinical yeasts; Culture collections; MALDI-TOF MS.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200020
Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas
Provedor de dados: 58
Autores: Nwinyi,Obinna C.; Ajayi,Oluseyi O.; Amund,Olukayode O.
ABSTRACT The goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize
polynuclear aromatic hydrocarbons of former industrial sites at McDoel Switchyard in Bloomington, Indiana. Using conventional
enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains PB1 and PB2. Applying
the ribotyping technique using the 16S rRNA gene analysis, the strains were assigned to the genus Pseudomonas (Pseudomonas
plecoglossicida strain PB1 and Pseudomonas sp. PB2). Both isolates showed promising metabolic capacity on pyrene sprayed MS
agar plates during the preliminary investigations. Using time course studies in the liquid cultures at calculated concentrations 123,
64, 97...
Tipo: Journal article
Palavras-chave: 16S rRNA gene analysis; Former industrial sites; Soil microbial communities; Competent PAH degraders.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300551
A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis
(Lepidoptera: Crambidae)
Provedor de dados: 58
Autores: Gitahy,Patrícia de Medeiros; Souza,Marlene Teixeira de; Monnerat,Rose Gomes; Arrigoni,Enrico de Beni; Baldani,José
Ivo.
The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a
long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane
borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity
towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the
LC50 of 13.06 µg/L and 143.88 µg/L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other
B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and...
Tipo: Journal article
Palavras-chave: Biological control; Bacillus thuringiensis kurstaki; Cry toxins; Spherical crystal; Diatraea saccharalis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300028
Comparative study of wild and transformed salt tolerant bacterial strains on Triticum aestivum
growth under salt stress
Provedor de dados: 58
Autores: Afrasayab,Shazia; Faisal,Muhammad; Hasnain,Shahida.
Eleven salt tolerant bacteria isolated from different sources (soil, plants) and their transformed strains were used to study their
influence on Triticum aestivum var. Inqlab-91 growth under salt (100 mM NaCl) stress. Salt stress caused reduction in
germination (19.4%), seedling growth (46%) and fresh weight (39%) in non-inoculated plants. In general, both wild and
transformed strains stimulated germination, seedling growth and fresh weight in salt free and salt stressed conditions. At 100 mM
NaCl, Staphylococcus xylosus ST-1 caused 25% increments in seedling length over respective control. Soluble protein content
significantly enhanced (49%) under salt stress as compared to salt free control. At 100 mM NaCl parental strain PT-5 resulted
about 32%...
Tipo: Journal article
Palavras-chave: Triticum aestivum; Plasmid; Rhizobacteria; Salinity; Auxin; Transformation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400013
Aeromonas associated diarrhoeal disease in south Brazil: prevalence, virulence factors and
antimicrobial resistance
Provedor de dados: 58
Autores: Guerra,Ivani M.F.; Fadanelli,Raquel; Figueiró,Manuela; Schreiner,Fernando; Delamare,Ana Paula L.;
Wollheim,Claudia; Costa,Sérgio Olavo P.; Echeverrigaray,Sergio.
Aeromonas were isolated from 27 (6.6%) of 408 patients admitted with acute gastroenteritis in two hospitals at Rio Grande do
Sul, Brazil. Isolates were classified as A. hydrophila (51.8%), A. caviae (40.8%), and A. veronii biotype sobria (7.4%). The
highest prevalence of Aeromonas associated infections occurred in lactants and children. Virulence genes (aerA
-aerolysin/hemolysin, ahpA -serine-protease, satA - glycerophospholipid-cholesterol acyltransferase, lipA -lipase, and ahyB
-elastase) and virulence factors (hemolytic, proteolitic, lipolitic activities, and biofilm formation) were identified in most A.
hydrophila and A. veronii biotype sobria isolates, with lower frequencies on A. caviae. All Aeromonas isolates were resistant to
ampicillin,...
Tipo: Journal article
Palavras-chave: Aeromonas; Diarrhea disease; Virulence factors; Antimicrobial resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400011
Bacterial treatment of alkaline cement kiln dust using Bacillus halodurans strain KG1
Provedor de dados: 58
Autores: Kunal,; Rajor,Anita; Siddique,Rafat.
Abstract This study was conducted to isolate an acid-producing, alkaliphilic bacterium to reduce the alkalinity of cement industry
waste (cement kiln dust). Gram-positive isolate KG1 grew well at pH values of 6–12, temperatures of
28–50 °C, and NaCl concentrations of 0–16% and thus was further screened for its potential to reduce
the pH of an alkaline medium. Phenotypic characteristics of the KG1 isolate were consistent with those of the genus Bacillus, and
the highest level of 16S rRNA gene sequence similarity was found with Bacillus halodurans strain DSM 497 (94.7%). On the
basis of its phenotypic characteristics and genotypic distinctiveness from other phylogenetic neighbors belonging to alkaliphilic
Bacillus species, the...
Tipo: Journal article
Palavras-chave: Alkaliphiles; Alkalinity reduction; Bacillus sp; Cement kiln dust.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100001
Hexavalent chromium reduction by aerobic heterotrophic bacteria indigenous to chromite mine
overburden
Provedor de dados: 58
Autores: Satarupa,Dey; Paul,A.K.
Microbiological analysis of overburden samples collected from chromite mining areas of Orissa, India revealed that they are rich
in microbial density as well as diversity and dominated by Gramnegative (58%) bacteria. The phenotypically distinguishable
bacterial isolates (130) showed wide degree of tolerance to chromium (2-8 mM) when tested in peptone yeast extract glucose agar
medium. Isolates (92) tolerating 2 mM chromium exhibited different degrees of Cr+6 reducing activity in chemically defined
Vogel Bonner (VB) broth and complex KSC medium. Three potent isolates, two belonging to Arthrobacter spp. and one to
Pseudomonas sp. were able to reduce more than 50 and 80% of 2 mM chromium in defined and complex media respectively.
Along with Cr+6 (MIC 8.6-17.8...
Tipo: Journal article
Palavras-chave: Chromite overburden; Hexavalent chromium; Chromate reduction; Arthrobacter; Pseudomonas.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100045
Systematic mutagenesis method for enhanced production of bacitracin by Bacillus licheniformis
mutant strain UV-MN-HN-6
Provedor de dados: 58
Autores: Aftab,Muhammad Nauman; Haq,Ikram-ul; Baig,Shahjahan.
The purpose of the current study was intended to obtain the enhanced production of bacitracin by Bacillus licheniformis through
random mutagenesis and optimization of various parameters. Several isolates of Bacillus licheniformis were isolated from local
habitat and isolate designated as GP-35 produced maximum bacitracin production (14±0.72 IU ml-1). Bacitracin production of
Bacillus licheniformis GP-35 was increased to 23±0.69 IU ml-1 after treatment with ultraviolet (UV) radiations. Similarly,
treatment of vegetative cells of GP-35 with chemicals like N-methyl N'-nitro N-nitroso guanidine (MNNG) and Nitrous acid
(HNO2) increased the bacitracin production to a level of 31±1.35 IU ml-1 and 27±0.89 IU ml-1 respectively. Treatment of isolate
GP-35 with...
Tipo: Journal article
Palavras-chave: Mutagenesis; Bacitracin; B. licheniformis; Optimization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100009
Synergistic haemolytic activity and its correlation to phospholipase D productivity by
Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes
Provedor de dados: 58
Autores: Selim,S.A.; Mousa,W.M.; Mohamed,K.F.; Moussa,I.M.
Fourteen isolates of Corynebacteruim pseudotuberculosis of them 7 were isolated from sheep with Caseous Lymphadenitis
"biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard
microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and phospholipase D genes. Synergistic
haemolytic titers of all isolates were assayed by plate technique. The presences of phospholipase D gene in supernatants of all
isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using
hyperimmune serum raised in rabbit immunized with recombinant phospholipase D gene antigen. The concentration of
phospholipase D gene was assayed by scanning the...
Tipo: Journal article
Palavras-chave: Corynebacteruim pseudotuberculosis; Oedematous Skin Disease; Caseous Lymphadenitis; Recombinant PLD;
Synergistic haemolysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200016
Glanders in donkeys (Equus asinus) in the state of Pernambuco, Brazil: a case report
Provedor de dados: 58
Autores: Mota,Rinaldo Aparecido; Oliveira,Andréa Alice da Fonseca; Pinheiro Junior,José Wilton; Silva,Leonildo Bento Galiza
da; Brito,Marilene de Farias; Rabelo,Silvana Suely Assis.
The clinical, anatomical and histopatological findings of glanders diagnosis in donkeys in the state of Pernambuco-Brazil are
reported. The animals were euthanized and necropsied, and evaluated for lesions in respiratory and lymphatic systems, confirming
the disease by isolation of Burkholderia mallei and Strauss test.
Tipo: Journal article
Palavras-chave: Burkholderia mallei; Epidemiology; Microbiology; Histopathology; Strauss test.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100021
Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a Chinese
shallow eutrophic urban lake
Provedor de dados: 58
Autores: Qiu,Shanlian; Chen,Guoyuan; Zhou,Yiyong.
The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the
abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu).
Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of
organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g-1 dry weight) in sediment
significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the
amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones
being common among all...
Tipo: Journal article
Palavras-chave: Ammonia-oxidizing bacteria; Abundance; AmoA; Diversity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100031
Prevalence, aetiology and antibiotic resistance profiles of coagulase negative staphylococci isolated in
a teaching hospital
Provedor de dados: 58
Autores: Keim,Luiz S; Torres-Filho,Sylvio R; Silva,Patricia Vollú; Teixeira,Lenise A.
In this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (CNS) isolates
found in an university hospital. Two hundred four CNS isolates from 191 patients obtained between the period of 1998 to 2002,
were studied. About 27% (52/191) of the infection cases studied were confirmed as CNS-associated diseases. Blood stream
infection (BSI) was the most frequent CNS associated-disease (25%; 13/52). The great majority of the BSI was verified in the
Neonatal Intensive Care Unit (NICU). The analysis of the 52 patients medical history showed that 85% of the BSI was acquired in
hospital. Most of the CNS nosocomial infections were associated with the use of indwelling medical devices. The incidence of...
Tipo: Journal article
Palavras-chave: Coagulase negative staphylococci; Nosocomial infection; Antimicrobial resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100031
Simultaneous and successive inoculations of yeasts and lactic acid bacteria on the fermentation of an
unsulfited Tannat grape must
Provedor de dados: 58
Autores: Muñoz,Viviana; Beccaria,Bruno; Abreo,Eduardo.
Interactions between yeasts and lactic acid bacteria are strain specific, and their outcome is expected to change in simultaneous
alcoholic -malolactic fermentations from the pattern observed in successive fermentations. One Oenococcus oeni strain Lalvin
VP41TM was inoculated with two Saccharomyces cerevisiae strains either simultaneously, three days after the yeast inoculation,
or when alcoholic fermentation was close to finish. Early bacterial inoculations with each yeast strain allowed for the growth of
the bacterial populations, and the length of malolactic fermentation was reduced to six days. Alcoholic fermentation by Lalvin
ICV D80® yeast strain left the highest residual sugar, suggesting a negative effect of the bacterial growth and malolactic...
Tipo: Journal article
Palavras-chave: Saccharomyces; Oenococcus; Co-inoculation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100009
Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter
meiyuanensis SYBC-H1
Provedor de dados: 58
Autores: Hao,Zhikui; Cai,Yujie; Liao,Xiangru; Zhang,Xiaoli; Fang,Zhiyou; Zhang,Dabing.
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil
recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising
of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in
which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in
the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were
performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
Tipo: Journal article
Palavras-chave: Chitinase; Medium optimization; Response surface method; Inulin; Chitinolyticbacter meiyuanensis SYBC-H1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019
Paralytic rabies in swine
Provedor de dados: 58
Autores: Pessoa,Clarice Ricardo de Macedo; Silva,Maria Luana Cristiny Rodrigues; Gomes,Albério Antônio de Barros;
Garcia,Andrea Isabel Estévez; Ito,Fumio Honma; Brandão,Paulo Eduardo; Riet-Correa,Franklin.
Rabies transmitted by vampire bats was diagnosed in pigs with paralysis of the pelvic limbs. Diffuse nonsuppurative
encephalomyelitis, affecting mainly the spinal cord, was observed histologically. Despite the various diagnosis of rabies in pigs
this is the first report of clinical signs and pathology of rabies transmitted by vampire bats.
Tipo: Journal article
Palavras-chave: Vampire bats; Epidemiology; Virus isolation; Zoonotic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100038
Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by
two-dimensional electrophoresis
Provedor de dados: 58
Autores: Di Ciero,Luciana; Bellato,Cláudia de M.; Meinhardt,Lyndel W.; Ferrari,Fernanda; Castellari,Rafael R.;
Marangoni,Sérgio; Novello,José C.
Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the
solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially
solubilized in distinct buffers containing the detergent and subjected to bidimensional electrophoresis within the non-linear pH
range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, respectively. CHAPS and
Triton X100 were less effective and revealed only 72 and 43 spots, respectively. MALDI-TOF tryptic peptide mass fingerprinting
of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theoretical efficiency
of...
Tipo: Journal article
Palavras-chave: X. fastidiosa; 2-DE; Extraction; Proteome; Solubilization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200018
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium
Provedor de dados: 58
Autores: Santisi,Santina; Cappello,Simone; Catalfamo,Maurizio; Mancini,Giuseppe; Hassanshahian,Mehdi; Genovese,Lucrezia;
Giuliano,Laura; Yakimov,Michail M.
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri
SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four
bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax:
Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas:
Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in
microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source).
Measures of total and live...
Tipo: Journal article
Palavras-chave: Alcanivorax; Pseudomonas; Rhodococcus; Bioremediation; Bioaugmentation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377
Enterotoxigenicity of Staphylococcus aureus isolated from traditional and commercial dairy products
marketed in Iran
Provedor de dados: 58
Autores: Rahimi,Ebrahim.
This study was conducted to determine the prevalence rate, enterotoxigenecity, and antimicrobial resistance of S. aureus isolated
from dairy products in Iran. From September 2010 to July 2011, a total of 347 samples from various dairy products, traditional
and commercial, were collected from randomly selected retail stores. Overall, 20 samples (5.8%) were found to be contaminated
with S. aureus. The highest prevalence of S. aureus was found in traditional cheese (11.1%), followed by traditional ice-cream
(5.9%), cream (5.6%), and butter (5.3%). The ability to synthesize classical staphylococcal enterotoxins (SEA-E) was determined
in 7 of 20 (35%) isolates by using ELISA. SE type C was the most common enterotoxin found in the isolated S. aureus (42.9%),...
Tipo: Journal article
Palavras-chave: Antimicrobial resistance; Dairy products; SE genes; Staphylococcal enterotoxins; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200008
Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing
dairy farms
Provedor de dados: 58
Autores: Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio;
Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto.
The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to
California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12
milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission
modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and
bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species
Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest
isolation frequency of Staphylococcus...
Tipo: Journal article
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050
Nosocomial candidiasis in Rio de Janeiro State: Distribution and fluconazole susceptibility profile
Provedor de dados: 58
Autores: Neufeld,Paulo Murillo; Melhem,Marcia de Souza Carvalho; Szeszs,Maria Walderez; Ribeiro,Marcos Dornelas;
Amorim,Efigênia de Lourdes Teixeira; Silva,Manuela da; Lazéra,Marcia dos Santos.
One hundred and forty-one Candida species isolated from clinical specimens of hospitalized patients in Rio de Janeiro, Brazil,
during 2002 to 2007, were analized in order to evaluate the distribution and susceptibility of these species to fluconazole. Candida
albicans was the most frequent species (45.4%), followed by C. parapsilosis sensu lato (28.4%), C. tropicalis (14.2%), C.
guilliermondii (6.4%), C. famata (2.8%), C. glabrata (1.4%), C. krusei (0.7%) and C. lambica (0.7%). The sources of fungal
isolates were blood (47.5%), respiratory tract (17.7%), urinary tract (16.3%), skin and mucous membrane (7.1%), catheter (5.6%),
feces (2.1%) and mitral valve tissue (0.7%). The susceptibility test was performed using the methodology of disk-diffusion in
agar...
Tipo: Journal article
Palavras-chave: Candida; Fluconazole; Antifungal susceptibility; Disk diffusion method.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200477
Molecular variations in Vibrio alginolyticus and V. harveyi in shrimp-farming systems upon stress
Provedor de dados: 58
Autores: Santhyia,Anix Vivek; Mulloorpeedikayil,Rosalind George; Kollanoor,Riji John; Jeyaseelan,Prince M.J.
A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi
when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern
Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and gyrB gene specific
PCR. The bacterial strains were genotyped by PCR fingerprinting using GTG(5) and IS (Insertion Sequence) primers. Seven
strains each of V. alginolyticus and V. harveyi were subjected to 10 passages through trypticase soya broth (TSB), which
contained different NaCl concentrations (3, 6 and 8%) and trypticase soya agar (TSA). V. alginolyticus was also passaged through
TSB with a 12%...
Tipo: Journal article
Palavras-chave: Vibrio harveyi; Vibrio alginolyticus; Molecular variation; Shrimp; Penaeus monodon.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401001
Carbaryl degradation by bacterial isolates from a soil ecosystem of the Gaza Strip
Provedor de dados: 58
Autores: Hamada,Mazen; Matar,Ammar; Bashir,Abdallah.
Abstract Carbaryl is an important and widely used insecticide that pollutes soil and water systems. Bacteria from the local soil
ecosystem of the Gaza Strip capable of utilizing carbaryl as the sole source of carbon and nitrogen were isolated and identified as
belonging to Bacillus, Morganella, Pseudomonas, Aeromonas and Corynebacterium genera. Carbaryl biodegradation by Bacillus,
Morganella and Corynebacterium isolates was analyzed in minimal liquid media supplemented with carbaryl as the only source of
carbon and nitrogen. Bacillus and Morganella exhibited 94.6% and 87.3% carbaryl degradation, respectively, while
Corynebacterium showed only moderate carbaryl degradation at 48.8%. These results indicate that bacterial isolates from a local
soil ecosystem...
Tipo: Journal article
Palavras-chave: Biodegradation; Soil bacterial isolates; Carbaryl; TLC.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401087
Isolation, identification and physiological study of Lactobacillus fermentum LPB for use as probiotic
in chickens
Provedor de dados: 58
Autores: Reque,Elizete de F.; Pandey,Ashok; Franco,Sebastião G.; Soccol,Carlos R.
Studies were carried out to isolate and identify microorganisms for probiotic use for chickens. Selection of strains included
various criteria such as agreement with bio-safety aspects, viability during storage, tolerance to low pH/ gastric juice, bile, and
antimicrobial activity. The strains were isolated from the crop, proventriculus, gizzard, ileum and caeca of chicken. Decimal
dilution of the contents of these segments were mixed with MRS medium and incubated for 48 h at 37°C under anaerobiosis. The
identity of the culture was based on characteristics of lactobacilli as presented in the Bergey’ s Manual of Determinative
Bacteriology, carrying out bacterioscopy (morphology), Gram stain, growth at 15 and 45°C, and fermentation of different carbon
sources....
Tipo: Journal article
Palavras-chave: Probiotic; Poultry; Lactobacillus fermentum.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400012
Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by
multiple-locus variable number-tandem repeat analysis
Provedor de dados: 58
Autores: Gioffré,Andrea; Correa Muñoz,Magnolia; Alvarado Pinedo,María F.; Vaca,Roberto; Morsella,Claudia; Fiorentino,María
Andrea; Paolicchi,Fernando; Ruybal,Paula; Zumárraga,Martín; Travería,Gabriel E.; Romano,María Isabel.
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP)
isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously
investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was
reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8
repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2,
INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns,
grouping 76.3% of the isolates. We were also...
Tipo: Journal article
Palavras-chave: Paratuberculosis; Molecular typing; MIRU-VNTR; MLVA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200557
Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates
Provedor de dados: 58
Autores: Dong-Ya,Meng; Chang-Jian,Sun; Jing-Bo,Yu; Jun,Ma; Wen-Cheng,Xue.
To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from
urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were
screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and
topoisomerase IV (parC and parE) in comparison with the reference strain PG21, which is susceptible to fluoroquinolones
antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these
quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB
and no mutations were found in the...
Tipo: Journal article
Palavras-chave: Mycoplasma hominis; Quinolones; Drug resistance; Mutation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100034
Changes in the microbiological and physicochemical characteristics of Serrano cheese during
manufacture and ripening
Provedor de dados: 58
Autores: Souza,Claucia Fernanda Volken de; Dalla Rosa,Tiziano; Ayub,Marco Antônio Zachia.
Changes in the microbial flora present in six Serrano cheese batches (3 ripened in Summer and 3 in Winter) were studied during
production and ripening, to microbiologically characterize this cheese, which is traditionally manufactured by farmers in the south
of Brazil, using raw cow milk, without the addition of starter cultures. The levels of several microbial groups were enumerated in
the milk, curd and different stages of the 60-day long ripening period. Values of some physicochemical parameters, such as pH,
titratable acidity, water activity, moisture and NaCl content were also determined. The correlation coefficients between these
parameters and the microbial groups throughout ripening were determined. The influence of the season of the year was also...
Tipo: Journal article
Palavras-chave: Serrano cheese; Cheese ripening; Food microbiology; Lactic acid bacteria.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300016
Application of representational difference analysis to identify genomic differences between
Bradyrhizobium elkanii and B. Japonicum species
Provedor de dados: 58
Autores: Soares,René Arderius; Passaglia,Luciane Maria Pereira.
Bradyrhizobium elkanii is successfully used in the formulation of commercial inoculants and, together with B. japonicum, it fully
supplies the plant nitrogen demands. Despite the similarity between B. japonicum and B. elkanii species, several works
demonstrated genetic and physiological differences between them. In this work Representational Difference Analysis (RDA) was
used for genomic comparison between B. elkanii SEMIA 587, a crop inoculant strain, and B. japonicum USDA 110, a reference
strain. Two hundred sequences were obtained. From these, 46 sequences belonged exclusively to the genome of B. elkanii strain,
and 154 showed similarity to sequences from B. japonicum genome. From the 46 sequences with no similarity to sequences from
B. japonicum, 39...
Tipo: Journal article
Palavras-chave: Bradyrhizobium; Genome comparison; Nitrogen fixation; Representational difference analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400036
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp.
paratuberculosis
Provedor de dados: 58
Autores: Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.;
Cravero,Silvio L.
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through
isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for
MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar
to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye
detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification
scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols...
Tipo: Journal article
Palavras-chave: Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619
Screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of
Remazole Marine Blue
Provedor de dados: 58
Autores: Erden,Emre; Ucar,M. Cigdem; Gezer,Tekin; Pazarlioglu,Nurdan Kasikara.
This study presents new and alternative fungal strains for the production of ligninolytic enzymes which have great potential to use
in industrial and biotechnological processes. Thirty autochthonous fungal strains were harvested from Bornova-Izmir in Turkiye.
In the fresh fruitbody extracts laccase, manganese peroxidase and lignin peroxidase activities, which are the principal enzymes
responsible for ligninocellulose degradation by Basidiomycetes, were screened. Spores of some of the basidiomycetes species
such as Cortinarius sp., Trametes versicolor, Pleurotus ostreatus, Abortiporus biennis, Lyophyllum subglobisporium, Ramaria
stricta, Ganoderma carnosum, Lactarius delicious ve Lepista nuda were isolated and investigated optimum cultivation conditions
in...
Tipo: Journal article
Palavras-chave: Basidiomycetes; Ligninolytic enzymes; Decolorization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200026
Measuring bacterial cells size with AFM
Provedor de dados: 58
Autores: Osiro,Denise; Bernardes Filho,Rubens; Assis,Odilio Benedito Garrido; Jorge,Lúcio André de Castro; Colnago,Luiz
Alberto.
Atomic Force Microscopy (AFM) can be used to obtain high-resolution topographical images of bacteria revealing surface details
and cell integrity. During scanning however, the interactions between the AFM probe and the membrane results in distortion of
the images. Such distortions or artifacts are the result of geometrical effects related to bacterial cell height, specimen curvature
and the AFM probe geometry. The most common artifact in imaging is surface broadening, what can lead to errors in bacterial
sizing. Several methods of correction have been proposed to compensate for these artifacts and in this study we describe a simple
geometric model for the interaction between the tip (a pyramidal shaped AFM probe) and the bacterium (Escherichia coli
JM-109...
Tipo: Journal article
Palavras-chave: Atomic force microscopy (AFM); Escherichia coli; Cell dimensions; Bacteria visualization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100040
Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela
Provedor de dados: 58
Autores: Huang,Haibin; Unver,Ahmet; Perez,Miriam J.; Orellana,Nelson G.; Rikihisa,Yasuko.
Blood specimens from clinically normal military dogs and their trainers, in Lara, Venezuela were screened for Anaplasma platys,
A. phagocytophilum, or Ehrlichia ewingii using 16S rRNA PCR tests. Sixteen percent (7/43) of dog specimens were positive by
A. platys PCR test followed by sequencing of the PCR products, and all human blood specimens [25] were negative. All
specimens from these dogs and humans were PCR negative for E. ewingii or A. phagocytophilum. Twelve Rhipicephalus
sanguineus ticks removed from these dogs were negative for A. platys by reverse transcription PCR test. Almost the entire 16S
rRNA gene (1,364 bp) and groESL operon (1,646 bp) sequences of A. platys isolated from a dog were determined, revealing that
both sequences were closely...
Tipo: Journal article
Palavras-chave: 16S rRNA; GroESL operon; Anaplasma platys; Dogs; Venezuela.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300002
Homology among extra-cryptic DNA bands and the typical plasmids in Brazilian Yersinia pestis
strains
Provedor de dados: 58
Autores: Leal,Nilma Cintra; Sobreira,Marise; Leal,Tereza Cristina Arcanjo; Almeida,Alzira Maria Paiva de.
Yersinia pestis, the etiologic agent of plague, harbors three well-characterized plasmids: pFra (90-110kb), pYV (70 kb) and pPst
(9.5 kb). Furthermore, some extra-cryptic DNA bands have been observed in a number of wild strains from several foci of the
world. Additional bands have also been reported in Brazilian strains. Looking for any relationship among these cryptic DNA
bands and the three-prototypical plasmids, we analyzed twelve strains displaying different plasmid content. The DNA bands were
hybridized by southern blot with probes directed at the genes caf1, lcrV and pla located respectively on the plasmids pFra, pYV
and pPst. The probes were constructed by PCR amplification and labeled with digoxigenin. The Pla probe hybridized with its
target...
Tipo: Journal article
Palavras-chave: Y. pestis; Plasmids; Probes; Virulence genes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100006
Epidemiology and phospholipase activity of oral Candida spp. among patients with central nervous
system diseases before and after dental cleaning procedure
Provedor de dados: 58
Autores: Ribeiro,Aurélia Silva; Silva,Dágma Aparecida; Silva,Francislene Pereira; Santos,Gleicy Carla; Campos,Lívia Maria
Soares; Oliveira,Lorena Vivien Neves; Santos,Daniel Assis.
Patients suffering of diseases that affect central nervous system may be considered more susceptible to the infectious diseases of
mouth. Sixty-nine patients suffering of cerebral palsy, Down's syndrome and metal retardation were submitted to saliva
examination for the presence of Candida spp. before and after a procedure of dental cleaning. The isolates were submitted to assay
for verifying phospholipase production. 55.10% of the patients provided isolation of Candida spp. The frequency of isolation
obtained before dental procedure was: C. albicans (83.33%), C. krusei (8.33%) and C. kefyr, C. parapsilosis and C. glabrata
(2.78% each). The frequency after the procedure was: C. albicans (68.57%), C. parapsilosis (11.43%), C. krusei and C. kefyr
(8.57% each)...
Tipo: Journal article
Palavras-chave: Candida spp.; Cerebral palsy; Down's syndrome; Metal retardation; Phospholipase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100004
A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species
Provedor de dados: 58
Autores: Radhika,M.; Saugata,Majumder; Murali,H.S.; Batra,H.V.
Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal
diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous
detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1,
ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica
respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella
spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae...
Tipo: Journal article
Palavras-chave: Salmonella; Shigella; Multiplex PCR; Spiking.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200041
First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in
Southern Brazil
Provedor de dados: 58
Autores: Gusatti,Carolina de Souza; Bertholdo,Lauren Martins; Otton,Letícia Muner; Marchetti,Desirée Padilha;
Ferreira,Alessandra Einsfeld; Corção,Gertrudes.
This is the first report of an Acinetobacter baumannii from clinical origin carrying the blaOXA-58 gene in Brazil. The isolate
included in this study was from a patient during an outbreak in Porto Alegre, RS, Southern Brazil, in 2007. It was resistant to most
of the beta-lactams tested, it has also the blaOXA-65 gene and the ISAba1 sequence located upstream to both blaOXA genes
detected and it has a MIC of imipenem of 64 μg/mL.
Tipo: Journal article
Palavras-chave: BlaOXA-58; ISAba1; BlaOXA-65; Carbapenemases; Acinetobacter baumannii.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100027
Gram-negative intestinal indigenous microbiota from two Siluriform fishes in a tropical reservoir
Provedor de dados: 58
Autores: Duarte,Silvana; Silva,Flávia Cristina de Paula e; Zauli,Danielle Alves Gomes; Nicoli,Jacques Robert; Araújo,Francisco
Gerson.
The Gram-negative intestinal microbiota of Hypostomus auroguttatus and Pimelodus maculatus, a detritivorous and an
omnivorous fish species, respectively, were compared between fishes from the reservoir and the stretch of the river below the dam
of the Funil hydroelectric plant, Rio de Janeiro, Brazil. Four selective culture media were used under aerobic and two under
anaerobic conditions. The omnivorous species had microbiota with higher population levels compared to the detritivorous species.
The number of morphotypes and population levels of total bacteria, vibrio and Bacteroides tended to be higher in summer and
autumn in the reservoir, and not different in the river. The number of morphotypes of enterobacteria and total bacteria were higher
in the...
Tipo: Journal article
Palavras-chave: Alimentary regimen; Environment; Gut microbiology; Hypostomus auroguttatus; Pimelodus maculatus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400019
Immune response elicited by the oral administration of an intermediate strain of IBDV in chickens
Provedor de dados: 58
Autores: Carballeda,Juan Manuel; Zoth,Silvina Chimeno; Gómez,Evangelina; Lucero,María Soledad; Gravisaco,María José;
Berinstein,Analía.
The immune response elicited by the oral inoculation of an intermediate strain of infectious bursal disease virus was studied in
chickens. A strong over expression of IL-6, IL-8, IFNα and IFNγ was observed in bursa at 3 days post
inoculation together with an increase in splenic NO2 release. An influx of T-lymphocytes was also detected.
Tipo: Journal article
Palavras-chave: Infectious Bursal Disease virus; Innate immunity; Avian cytokines; Flow cytometry; RT-qPCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400049
Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci
isolated from nosocomial specimens
Provedor de dados: 58
Autores: Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de.
Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin),
collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and
API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin
susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6
µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S.
epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental
isolates was similar...
Tipo: Journal article
Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010
Intralaboratory optimization and validation of a method for patulin determination in grapes by
Thin-Layer Chromatography
Provedor de dados: 58
Autores: Oliveira,Renata Cabrera de; Bando,Érika; Machinski Junior,Miguel.
The aim of this work was to optimize and validate, by intralaboratorial procedures, a method for the determination of patulin in
grapes by thin-layer chromatography. The steps of extraction, cleanup, detection and quantification were optimized. For the
validation of the method, recovery assays with standard solutions and artificially contaminated samples were carried out. The
mean recovery and the variation coefficient were 65.4% and 7.58%, respectively. The optimized conditions were: 50 mL of grape
juice, three extraction stages (with 100 mL of ethyl acetate in the first stage and 50 mL in second and third stages), and 100 µL of
ethanol to solubilize the extract. The solvent-system used was toluene - ethyl acetate - formic acid (6:3:1), and 0.5% MBTH in...
Tipo: Journal article
Palavras-chave: Patulin; Mycotoxins; Grape; Thin-layer chromatography.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200023
Validation of a predictive model describing growth of Salmonella in enteral feeds
Provedor de dados: 58
Autores: Silva,Roberta Ribeiro; Moraes,Célia Alencar; Bessan,Josefina; Vanetti,Maria Cristina Dantas.
The growth of Salmonella enterica subs. enterica sorovar Typhimurium at 25°C was monitored in industrialized and hospital
formulated enteral feeds and the results were used to validate the mathematical model of Salmonella growth presented by the
Pathogen Modeling Program (PMP) 7.0 (USDA-USA). The generation time of Salmonella in enteral feeds ranged from 21 to 34.8
min and, the maximum growth rate (µmax) varied from 1.28 to 1.95 h-1, resulting in a population increase from 5 to 6 log10
cycles within 14 to 24 h incubation. Growth was faster in the hospital formulated feed containing vegetables and eggs. The growth
kinetic's parameters as lag phase; µmax and maximum population density (MPD) were similar to those predicted by the PMP 7.0,
with exception of...
Tipo: Journal article
Palavras-chave: Predictive microbiology; Salmonella growth; Enteral diets.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100026
The application of PCR in the detection of mycotoxigenic fungi in foods
Provedor de dados: 58
Autores: Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and
carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The
highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated
clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds
themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying
fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Journal article
Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
Characterization of alkaline xylanases from Bacillus pumilus
Provedor de dados: 58
Autores: Duarte,Marta Cristina Teixeira; Pellegrino,Ana Carolina Alcazar; Portugal,Edilberto Princi; Ponezi,Alexandre Nunes;
Franco,Telma Teixeira.
Alkaline xylanases produced by four different strains of Bacillus pumilus were characterized. The optimal pH and temperature
were pH 9.0 and 60ºC for strain 13a, and pH 8.0 and 55ºC for strains 5(2), 5(14), and 4a. Under these conditions the following
activities were found after 10 min in the presence of 1% xylan (birchwood): 328 U.ml-1, 131 U.ml-1, 90 U.ml-1, and 167 U.ml-1,
respectively, for the four strains. The enzymes were stable at 40ºC, with 40% of the xylanase activity remaining after 2 hours for
the enzymes of strain 5(2) and 60% for the other three strains. Stability at 50ºC was improved by addition of glycerol. Taking into
account the conditions under which kraft pulps are bleached during the manufacture of paper, xylanases from B. pumilus...
Tipo: Journal article
Palavras-chave: Xylan xylanase; B pumilus kraft pulp bleaching.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200005
Salmonella spp. in raw broiler parts: occurrence, antimicrobial resistance profile and phage typing of
the Salmonella Enteritidis isolates
Provedor de dados: 58
Autores: Ribeiro,Aldemir Reginato; Kellermann,Aline; Santos,Luciana Ruschel dos; Bessa,Marjo Cadó; Nascimento,Vladimir
Pinheiro do.
The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and to determine the
antimicrobial resistance profile of the isolated strains. Twenty-four (39.3%) broiler parts samples were positive for Salmonella and
twenty-five Salmonella strains were isolated, since two different serovars were detected in one single positive sample. Salmonella
Enteritidis was the most prevalent serovar. Among Salmonella Enteritidis isolates, 95.2% belonged to Phage Type 4 (PT4) (20/21)
and 4.8% to PT7 (1/21). Twenty-two (88%) strains of Salmonella were resistant to at least one antimicrobial agent, generating
eight different resistance patterns. The S. Typhimurium (n: 1) and S. Hadar (n: 3) isolates presented multiple resistance. Three...
Tipo: Journal article
Palavras-chave: Salmonella; Broiler parts; Antimicrobial resistance; Phage typing.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200021
Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Provedor de dados: 58
Autores: Puentes,R; Eliopulos,N; Pérez,R; Franco,G; Sosa,K; Bianchi,P; Furtado,A; Hübner,S.O.; Esteves,P.A.
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was
detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to
infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic
region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and
Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The
comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells
cultures and on CRFK line cells,...
Tipo: Journal article
Palavras-chave: Canine Parvovirus 2c; Isolation; Molecular characterization; South America.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300022
Growth rate inhibition of phytopathogenic fungi by characterized chitosans
Provedor de dados: 58
Autores: Oliveira Junior,Enio N.; Gueddari,Nour E. El; Moerschbacher,Bruno. M.; Franco,Telma T.
The inhibitory effects of fifteen chitosans with different degrees of polymerization (DP) and different degrees of acetylation (F A)
on the growth rates (GR) of four phytopathogenic fungi (Alternaria alternata, Botrytis cinerea, Penicillium expansum, and
Rhizopus stolonifer) were examined using a 96-well microtiter plate and a microplate reader. The minimum inhibitory
concentrations (MICs) of the chitosans ranged from 100 µg × mL-1 to 1,000 µg × mL-1 depending on the fungus tested and the
DP and F A of the chitosan. The antifungal activity of the chitosans increased with decreasing F A. Chitosans with low F A and
high DP showed the highest inhibitory activity against all four fungi. P. expansum and B. cinerea were relatively less susceptible
while A....
Tipo: Journal article
Palavras-chave: Chitosan; Antifungal activity; Fungal morphology; Phytopathogenic fungi.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200046
Quantitative evaluation of Listeria monocytogenes in fresh and processed surubim fish
(Pseudoplatystoma sp)
Provedor de dados: 58
Autores: Souza,V.M. de; Alves,V.F.; Destro,M.T.; De Martinis,E.C.P.
L. monocytogenes is a foodborne psychrotrophic bacterial pathogen of special importance for minimally processed foods. In this
work, it was enumerated in samples of surubim fish by MPN technique. The population of L. monocytogenes was estimated as
< 0.012 MPN/cm² in fresh and < 0.03 MPN/g in minimally processed fish.
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; MPN; Fish; Surubim.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300023
Epidemiology and antimicrobial resistance of staphylococci isolated from different infectious diseases
Provedor de dados: 58
Autores: Gad,Gamal Fadl M.; El-Ghafar,Abd El-Ghafar F. Abd; El-Domany,Ramadan A. A.; Hashem,Zeinab Shawky.
A total of 187 isolates from 470 clinical specimens were collected from three hospitals in El-Minia governorate and identified as
132 Staphylococcus aureus strains and 55 coagulase-negative staphylococci (CoNS) strains. Susceptibility of isolates to
antimicrobial agents was tested by the agar dilution method. The isolated S. aureus strains showed low resistance to vancomycin
(1.5%), amikacin (2.3%) and gatifloxacin (3.8%). Vancomycin was the most effective antibiotic against CoNS. The
ampicillin-resistant isolates were tested for β-lactamase production where, 61.7% of S. aureus and 42.9% of CoNS
were positive for β-lactamase enzyme. Beta-lactamase producing strains were screened for their plasmid profile using
alkaline lysis method. Some...
Tipo: Journal article
Palavras-chave: β -lactamase CCCP fluorometric assay efflux DAA technique.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200012
Extended-spectrum beta-lactamase-producing bacteria isolated from hematologic patients in Manaus,
State of Amazonas, Brazil
Provedor de dados: 58
Autores: Ferreira,Cristina Motta; Ferreira,William Antunes; Almeida,Nayanne Cristina Oliveira da Silva; Naveca,Felipe Gomes;
Barbosa,Maria das Graças Vale.
Antibiotic therapy in hematologic patients, often weak and susceptible to a wide range of infections, particularly nosocomial
infections derived from long hospitalization periods, is a challenging issue. This paper presents ESBL-producing strains isolated
from such hematologic patients treated at the Amazon Hematology and Hemotherapy Foundation (HEMOAM) in the Brazilian
Amazon Region to identify the ESBL genes carried by them as well as the susceptibility to 11 antimicrobial agents using the
E-test method. A total of 146 clinical samples were obtained from July 2007 to August 2008, when 17 gram-negative strains were
isolated in our institution. The most frequent isolates confirmed by biochemical tests and 16S rRNA sequencing were E. coli
(8/17), Serratia...
Tipo: Journal article
Palavras-chave: ESBL; Beta-lactams; Nosocomial infection; TEM; SHV; OXA; CTX-M.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300028
Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential
Provedor de dados: 58
Autores: Oliveira,Maíra Maciel Mattos de; Brugnera,Danilo Florisvaldo; Alves,Eduardo; Piccoli,Roberta Hilsdorf.
An experimental model was proposed to study biofilm formation by Listeria monocytogenes ATCC 19117 on AISI 304 (#4)
stainless steel surface and biotransfer potential during this process. In this model, biofilm formation was conducted on the surface
of stainless steel coupons, set on a stainless steel base with 4 divisions, each one supporting 21 coupons. Trypic Soy Broth was
used as bacterial growth substrate, with incubation at 37 ºC and stirring of 50 rpm. The number of adhered cells was determined
after 3, 48, 96, 144, 192 and 240 hours of biofilm formation and biotransfer potential from 96 hours. Stainless steel coupons were
submitted to Scanning Electron Microscopy (SEM) after 3, 144 and 240 hours. Based on the number of adhered cells and SEM, it
was...
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Biofilm; Biotransfer potential.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100016
Triple inoculation with Bradyrhizobium, Glomus and Paenibacillus on cowpea (Vigna unguiculata
[L.] walp.) development
Provedor de dados: 58
Autores: Lima,André Suêldo Tavares de; Xavier,Terezinha Ferreira; Lima,Cláudia Elizabete Pereira de; Oliveira,José de Paula;
Mergulhão,Adália Cavalcanti do Espírito Santo; Figueiredo,Márcia do Vale Barreto.
The use of microorganisms to improve the availability of nutrients to plants is of great importance to agriculture. This study aimed
to evaluate the effect of triple inoculation of cowpea with arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria
(PGPB) and rhizobia to maximize biological nitrogen fixation (BNF) and promote plant growth. The experiment was conducted in
a greenhouse using cowpea plants (Vigna unguiculata L. Walp cv. IPA 206). The treatments included inoculation with strains of
Bradyrhizobium sp. (BR 3267 and EI - 6) individually and as a mixture, an absolute control (AC) and mineral nitrogen control
(NC), all combined with the presence or absence of native AMF (Glomus etunicatum) and PGPB (Paenibacillus brasilensis - 24)
in a...
Tipo: Journal article
Palavras-chave: Arbuscular mycorrhiza; Biological nitrogen fixation; PGPB; Rhizobia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300010
Humic fractions of forest, pasture and maize crop soils resulting from microbial activity
Provedor de dados: 58
Autores: Tavares,Rose Luiza Moraes; Nahas,Ely.
Humic substances result from the degradation of biopolymers of organic residues in the soil due to microbial activity. The
objective of this study was to evaluate the influence of three different ecosystems: forest, pasture and maize crop on the formation
of soil humic substances relating to their biological and chemical attributes. Microbial biomass carbon (MBC), microbial
respiratory activity, nitrification potential, total organic carbon, soluble carbon, humic and fulvic acid fractions and the rate and
degree of humification were determined. Organic carbon and soluble carbon contents decreased in the order: forest > pasture
> maize; humic and fulvic acids decreased in the order forest > pasture=maize. The MBC and respiratory activity were
not...
Tipo: Journal article
Palavras-chave: Humification; Microbial biomass carbon; Potential nitrification; Respiratory activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300028
Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth
promoting activity of Leclercia sp. QAU-66
Provedor de dados: 58
Autores: Naveed,Muhammad; Ahmed,Iftikhar; Khalid,Nauman; Mumtaz,Abdul Samad.
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline
quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere
of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus
vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root
lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam,
InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline
quinone domains...
Tipo: Journal article
Palavras-chave: Glucose dehydrogenase; Pyrroloquinoline quinone; Leclercia sp; PGPR; Bioinformatics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200031
Comparative evaluation of the Nitrate Reductase Assay and the Resazurin Microtitre Assay for drug
susceptibility testing of Mycobacterium tuberculosis against first line anti-tuberculosis drugs
Provedor de dados: 58
Autores: Sanchotene,Karine O.; von Groll,Andrea; Ramos,Daniela; Scholante,Ana B.; Honscha,Gunther; Valença,Mariana;
Scaini,Carlos J.; Silva,Pedro E.A. da.
Tuberculosis remains as a serious infection disease of worldwide distribution, with high morbidity and mortality, mainly in low
socio-economic condition countries. The state of emergency of tuberculosis caused by the resistant and multidrug-resistant (MDR)
strains, became the main threat to the tuberculosis treatment and control programs. A fast detection method for the resistant strains
will allow the implementation of an adequate treatment and contribute for controlling the dissemination of these resistant strains.
This study evaluated the performance of the nitrate reductase assay in solid (NRA-LJ) and liquid (NRA-7H9) media, to determine
the susceptibility to first line anti-tuberculosis drugs: isoniazid (INH), rifampicin (RMP), ethambutol (EMB) and...
Tipo: Journal article
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; Nitrate reductase assay; Susceptibility tests; Drug resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100004
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera:
tephritidae)
Provedor de dados: 58
Autores: Marcon,Helena Sanches; Coscrato,Virgínia Elias; Selivon,Denise; Perondini,André Luiz Paranhos; Marino,Celso Luis.
Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and
60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male
killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using
primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer
used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR
conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In
this...
Tipo: Journal article
Palavras-chave: Anastrepha; Cross reaction; FtsZ; Wsp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200046
A toxic cyanobacterial bloom in an urban coastal lake, Rio Grande do Sul state, Southern Brazil
Provedor de dados: 58
Autores: Carvalho,Luciana Retz de; Pipole,Fernando; Werner,Vera Regina; Laughinghouse IV,Haywood Dail; Camargo,Antonio
Carlos M. de; Rangel,Marisa; Konno,Katsuhiro; Sant' Anna,Célia Leite.
Reports of cyanobacterial blooms developing worldwide have considerably increased, and, in most cases, the predominant toxins
are microcystins. The present study reports a cyanobacterial bloom in Lake Violão, Torres, Rio Grande do Sul State, in January
2005. Samples collected on January 13, 2005, were submitted to taxonomical, toxicological, and chemical studies. The
taxonomical analysis showed many different species of cyanobacteria, and that Microcystis protocystis and Sphaerocavum cf.
brasiliense were dominant. Besides these, Microcystis panniformis, Anabaena oumiana,Cylindrospermopsis raciborskii, and
Anabaenopsis elenkinii f. circularis were also present. The toxicity of the bloom was confirmed through intraperitoneal tests in
mice, and chemical...
Tipo: Journal article
Palavras-chave: Cyanobacteria; Blooms; Anabaenopeptins; Microcystins.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400031
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against
Aanticarsia gemmatalis (Lepidoptera:Noctuidae)
Provedor de dados: 58
Autores: Bobrowski,Vera Lucia; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Fiuza,Lidia Mariana.
The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during
sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants
or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most
important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A.
gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity
to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in...
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Velvetbean caterpillar; Anticarsia gemmatalis; Cry1 gene; Delta-endotoxin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200006
Inhibitory effect of essential oils against Trichosporon ovoides causing Piedra Hair Infection
Provedor de dados: 58
Autores: Saxena,Seema; Uniyal,Veena; Bhatt,R.P.
Piedra, is an asymptomatic fungal infection of the hair shaft, resulting in the formation of nodules of different hardness on the
infected hair. The infection also known as Trichomycosis nodularis is a superficial fungal infection arising from the pathogen
being restricted to the stratum corneum with little or no tissue reaction. The nodules are a concretion of hyphae and fruiting bodies
of the fungus. Two varieties of Piedra may be seen, Black Piedra and White Piedra. The fungus Trichosporon ovoides is involved
in the occurrence of both types of Piedras. The purpose of this study was to examine the effectiveness of selected essential oils for
the control of growth of the fungus and to determine whether the antifungal effect was due to the major compounds...
Tipo: Journal article
Palavras-chave: Essential oils; Piedra hair infection; Antifungal activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400016
Trichosporon pullulans (Lidner) Diddens & Lodder isolated from the oral cavity of AIDS
patient
Provedor de dados: 58
Autores: Neves,Rejane Pereira; Cavalcanti,Maria Auxiliadora de Queiroz; Chaves,Guilherme Maranhão; Magalhães,Oliane
Maria Correia.
Trichosporon has been reported as a remarkable emerging pathogen and usually occurs in imunodrepressed patients. An
oropharyngeal secretion sample was collected from a patient with AIDS, at the Sector of Infectious and Parasitic Diseases,
Osvaldo Cruz University Hospital, Pernambuco State, Brazil. No yeast cells or arthroconidia were observed in the direct exam,
but a culture was positive for Trichosporon pullulans, which is being reported in an AIDS patient for the first time.
Tipo: Journal article
Palavras-chave: Trichosporon pullulans; AIDS.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300010
Treatment with some anti-inflammatory drugs reduces germ tube formation in Candida albicans
strains
Provedor de dados: 58
Autores: Rusu,Elena; Radu-Popescu,Manuela; Pelinescu,Diana; Vassu,Tatiana.
Candida albicans is an opportunistic dimorphic fungus that inhabits various host mucosal sites. It can cause both superficial and
serious systemic disease. Conversion from the yeast to the hyphal form has been associated with increased virulence and mucosal
invasiveness. The aim of this study was to investigate the effect of sodium diclofenac and aspirin on germs tube formation of
different Candida albicans strains. Prostaglandins may play an important role in fungal colonization. Nonsteroidal
anti-inflammatory drugs are inhibitors of the cyclooxygenase isoenzymes. These drugs specifically block the biosynthesis of
mammalian prostaglandins by inhibiting one or both of cyclooxygenase isoenzymes. In tests for germ tube formation sodium
diclofenac reduced the...
Tipo: Journal article
Palavras-chave: Candida; Germ tube; Diclofenac; Aspirin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400031
Drug resistance profiles and clonality of sporadic Shigella sonnei isolates in Ankara, Turkey
Provedor de dados: 58
Autores: Kacmaz,Birgul; Unaldi,Ozlem; Sultan,Nedim; Durmaz,Riza.
The aims of this study were to investigate drug resistance rates, types of extended spectrum beta lactamases (ESBLs), and
molecular epidemiological characteristics of 43 Shigella sonnei isolates. Ampicillin-sulbactam, amoxicillin-clavulanate,
chloramphenicol, and ciprofloxacin were the most active antibiotics. Five isolates harbored blaSHV-12, blaTEM-1 and
blaCTX-M-15. More than 90% of the isolates had an indistinguishable pulsotype.
Tipo: Journal article
Palavras-chave: Shigella sonnei; Extended spectrum beta-lactamase; Plasmid profile; Pulsed-field gel electrophoresis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300013
Incidence and multiplex PCR based detection of trichothecene chemotypes of Fusarium culmorum
isolates collected from freshly harvested Maize kernels in Southern India
Provedor de dados: 58
Autores: Venkataramana,M.; Shilpa,P.; Balakrishna,K.; Murali,H.S.; Batra,H.V.
Hundred Fusarium culmorum strains, isolated from freshly harvested maize grain samples from Southern parts of India, were
incubated in czapek-dox medium and analyzed for trichothecene (DON/NIV) production. The mPCR assay was standardized
targeting trichothecene metabolic pathway genes viz., Tri6, Tri7, Tri13 for detection of trichothecene (DON/NIV) chemotypes
and rDNA gene for specific detection of F. culmorum species. Primers for targeted genes were designed and used to predict
whether these isolates could produce deoxynivalenol/nivalenol, 94 isolates were able to produce DON/NIV by mPCR assay.
Chemical analysis of DON/NIV was carried out for mPCR positive isolates by high performance-thin layer chromatography
(HPTLC). To check the practical usefulness of...
Tipo: Journal article
Palavras-chave: Deoxynivalenol; Nivalenol; Multiplex PCR assay; HPTLC.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200009
Distribution of prokaryotic organisms in a tropical estuary influenced by sugar cane agriculture in
northeast Brazil
Provedor de dados: 58
Autores: Wolf,Lars; Schwalger,Berit; Knoppers,Bastiaan A.; Silva,Luiz Antonio Ferreira da; Medeiros,Paulo Ricardo Petter;
Pollehne,Falk.
In a joint Brazilian-German case study, distribution patterns of microorganisms were compared with environmental variables in
the tropical coastal Manguaba lagoon in northeast Brazil, which is situated downstream of several sugar cane processing plants .
16S rDNA and 16S rRNA single strand conformation polymorphism (SSCP) gene fingerprinting were used to follow the
composition and distribution of microorganisms throughout the salinity gradient of the lagoon. Potentially abundant
microorganisms were identified by sequencing representative SSCP bands. It could be demonstrated that the distribution of
microbes was in close relation to the physico-chemical environmental settings and followed a common scheme. In the in- and
outlet areas of the lagoon rather...
Tipo: Journal article
Palavras-chave: Tropical lagoon; Estuarine gradient; Microbial diversity; Sugar cane.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400007
Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system
Provedor de dados: 58
Autores: Dambros,Régia Maria Feltrin; Ribeiro,Bergman Moraes; Aguiar,Raimundo Wagner de S.; Schaefer,Rejane;
Esteves,Paulo Augusto; Perecmanis,Simone; Simon,Neide Lisiane; Silva,Nayara Cavalcante; Coldebella,Michele;
Ciacci-Zanella,Janice Reis.
Aujeszky' s disease (AD) is an infectious disease causing important economic losses to the swine industry worldwide. The disease
is caused by an alpha-herpesvirus, Aujeszky' s disease virus (ADV), an enveloped virus with a double stranded linear DNA
genome. The ADV genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to
the infection. The glycoprotein E (gE) is a non-essential protein and deletion of the gE gene has been used for the production of
marker vaccines. Aiming to develop molecular reagents for the production of a gE specific ELISA test, the gE gene was amplified
by PCR, cloned and expressed into a baculovirus expression system. The recombinant DNA vector pFastBac-gE-ADV was used
for site-specific...
Tipo: Journal article
Palavras-chave: Aujeszky; Glycoprotein E; Baculovírus; Recombinant.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300021
"A comparison between sugar consumption and ethanol production in wort by immobilized
Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent
Grain"
Provedor de dados: 58
Autores: Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.
The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii
DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were
investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for
brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one
with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts
studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae,
1.7% for S....
Tipo: Journal article
Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii;
Saccharomyces rouxii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025
Antifungal properties of Brazilian cerrado plants
Provedor de dados: 58
Autores: Souza,Lúcia Kioko Hasimoto e; Oliveira,Cecília Maria Alves de; Ferri,Pedro Henrique; Santos,Suzana Costa; Oliveira
Júnior,Juldásio Galdino de; Miranda,André Thiago Borges; Lião,Luciano Morais; Silva,Maria do Rosário Rodrigues.
Ethanolic extracts from leaves of Hyptis ovalifolia, H. suaveolens, H. saxatilis, Hyptidendrum canum, Eugenia uniflora, E.
dysenterica, Caryocar brasiliensis and Lafoensia pacari were investigated for their antifungal activity against dermatophytes. The
most effective plants were H. ovalifolia and E. uniflora, while Trichophyton rubrum was the most sensitive among the four
dermatophytes species evaluated. This study has demonstrated antifungal properties of Brazilian Cerrado plant extracts in "in
vitro" assays.
Tipo: Journal article
Palavras-chave: Dermatophytes; Antifungal activity; Plant extracts; Susceptibility tests.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300012
Impairments of mecA gene detection in bovine Staphylococcus spp.
Provedor de dados: 58
Autores: Melo,Dayanne Araújo de; Coelho,Irene da Silva; Motta,Cássia Couto da; Rojas,Anna Carolina Coelho Marín;
Dubenczuk,Felipe Carlos; Coelho,Shana de Mattos de Oliveira; Souza,Miliane Moreira Soares de.
Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd
environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the
conserved regions from distinct host origins and also investigated the presence of homologue mecA LGA251 in bovine strains. A
total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine
coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin
agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA LGA251 gene. For mecA gene PCR, new
primers were designed based on...
Tipo: Journal article
Palavras-chave: Antimicrobial resistance; Bovine mastitis; Staphylococccus; MecA gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300041
Water quality and diversity of yeasts from tropical lakes and rivers from the Rio Doce basin in
Southeastern Brazil
Provedor de dados: 58
Autores: Medeiros,Adriana O.; Missagia,Beatriz S.; Brandão,Luciana R.; Callisto,Marcos; Barbosa,Francisco A. R.; Rosa,Carlos
A.
Yeast communities were assessed in 14 rivers and four lakes from the Doce River basin in Brazil, during the rainy and dry seasons
of the years 2000 and 2001. Water samples were collected at the subsurface in all sites. The following physical and chemical
parameters were measured: temperature, dissolved oxygen, pH, electrical conductivity, total phosphorus, ortho-phosphate,
ammonium, nitrate, nitrite and total nitrogen and the counts of faecal coliforms and heterotrophic bacteria were carried out to
characterize the aquatic environmental sampled. The yeast counts were higher in aquatic environments with the highest counts of
coliform and heterotrophic bacteria. These environments receive a high influx of domestic and industrial waste. A total of 317...
Tipo: Journal article
Palavras-chave: Tropical freshwater; Yeast diversity and water quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400043
DROUGHT-INDUCED EFFECTS AND RECOVERY OF NITRATE ASSIMILATION AND
NODULE ACTIVITY IN COWPEA PLANTS INOCULATED WITH BRADYRHIZOBIUM SPP.
UNDER MODERATE NITRATE LEVEL
Provedor de dados: 58
Autores: Silveira,Joaquim Albenísio Gomes da; Costa,Roberto Cezar Lobo da; Oliveira,José Tadeu Abreu.
This study was carried out to establish comparative effects of drought and recovery on the nitrate assimilation and nodule activity
related to N2 fixation in cowpea plants [Vigna unguiculata L. (Walp.)] previously inoculated with Bradyrhizobium spp. BR-3256
(CB-756) strain in the presence of 5 mol m-3 NO-3. Twenty-eight-day-old nodulated plants were submitted to water deprivation
during 4 consecutive days and afterwards resupplied with nutrient solution during 2 days. The water deprivation caused a rapid
increase in the nitrate content in root and a marked reduction in leaf nitrate reductase (NR) activity. In contrast nodule NR activity
was slightly increased by water deprivation. Concomitantly, in nodules of water stressed plants, leghemoglobin and...
Tipo: Journal article
Palavras-chave: Glutamine synthetase; Leghemoglobin; Nitrate reductase; N2 fixation; Water stress; Vigna unguiculata (L.)
Walp.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300005
Effect of surfactants and temperature on germination and vegetative growth of Beauveria bassiana
Provedor de dados: 58
Autores: Mwamburi,Lizzy A.; Laing,Mark D.; Miller,Ray M.
Three non-ionic surfactants: Tween20, Tween80 and Breakthru® were screened for their effects on spore germination and
mycelial growth rates and for their influence on three isolates of Beauveria bassianaspore germination at various temperatures.
Tween20 and Tween80 were compatible with all the B. bassiana isolates in the germination studies, but inhibited germination at
higher surfactant concentrations, irrespective of the conidial concentrations. Breakthru® had an inhibitory effect on germination
even at the lowest concentration of 0.1% on all the B. bassiana isolates. The effects of the surfactants on spore germination did not
correspond with their effects on colony growth. Conidial viability within the same formulation declined significantly with...
Tipo: Journal article
Palavras-chave: Beauveria bassiana; Surfactant; Temperature; Conidia germination; Mycelial growth.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100067
Detection of salmonella sp in chicken cuts using immunomagnetic separation
Provedor de dados: 58
Autores: Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos;
Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the
time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of
a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional
plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against
lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from
pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S....
Tipo: Journal article
Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034
Lectin staining patterns in human gastric mucosae with and without exposure to Helicobacter pylori
Provedor de dados: 58
Autores: Melo-Junior,Mario R.; Cavalcanti,Carmelita L.B.; Pontes-Filho,Nicodemos T.; Carvalho Jr.,Luiz B.; Beltrão,Eduardo I.
C.
The aim of the present study was to evaluate qualitative changes in the glycoconjugate expression in human gastric tissue of
positive and negative patients for Helicobacter pylori, through lectins: Wheat Germ Agglutinin (WGA) and Concanavalin A (Con
A). The lectins recognized differently the glycoconjugates in the superficial mucous layer at the gastric tissues. The results suggest
a significant change in the carbohydrate moieties present on the surface of the gastric cells during infection.
Tipo: Journal article
Palavras-chave: Lectin; Gastric mucosae; Helicobacter pylori.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200007
Aflatoxins, ochratoxin A and zearalenone in maize-based food products
Provedor de dados: 58
Autores: Sekiyama,Beatriz Leiko; Ribeiro,Alessandra Braga; Machinski,Paulo André; Machinski Junior,Miguel.
The occurrence of aflatoxins B1 (AFB1), B2 (AFB2 ), G1 (AFG1 ) and G2 (AFG2 ), ochratoxin A (OA) and zearalenone (ZEA)
was evaluated in 121 maize-based food samples collected in the commerce of Maringá City, Paraná State, Brazil. The study was
carried out between January 2002 and February 2003. Thin-layer chromatographic method was used to determine the mycotoxins.
The recovery averages were 106.6%, 109.4%, 106.6%, 109.4%, 101.8% and 101.7% to AFB1, AFB2, AFG1, AFG2, OA and
ZEA, respectively. Three samples (2.5%) were positive to AFB1 (8 to 59 µg/kg), two (1.7%) to AFB2 (2.4 µg/kg), one (0.8%) to
OA (64 µg/kg) and one (0.8%) to ZEA (448 µg/kg). The greater frequency of positive samples and also the highest concentration
of AFB1 were found in popcorn...
Tipo: Journal article
Palavras-chave: Mycotoxins; Risk; Brazilian products derived from maize.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300016
Batch ethanol fermentation: the correlation between the fermentation efficiency and the biomass
initial concentration depends on what is considered as produced ethanol
Provedor de dados: 58
Autores: Borzani,Walter.
Although numerous studies have examined many of the factors that affect the efficiency of batch ethanol fermentation, little
attention has been paid to the influence of the biomass concentration on this efficiency. This paper shows that the influence of the
biomass initial concentration on the fermentation efficiency depends on what is considered "produced ethanol". If only the ethanol
present in the medium aqueous phase at fermentation completion is considered, the fermentation efficiency linearly decreases
when the biomass initial concentration increases. If, however, the intracellular ethanol is also considered as produced ethanol, the
fermentation efficiency is not affected by the biomass concentration.
Tipo: Journal article
Palavras-chave: Ethanol fermentation efficiency; Intracellular ethanol; Biomass concentration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100016
Factors affecting the thermal activation of Neosartorya fischeri in pineapple and papaya nectars
Provedor de dados: 58
Autores: Slongo,Adriana Paula; Aragão,Gláucia Maria Falcão de.
In this research factors that influence the activation of Neosartorya fischeri ascospores (heating medium, temperature of
production and age of the ascospores) were studied. The heating medium used was pineapple and papaya nectars at different ratios
(ºBrix/acidity). Lower activation times were observed for the N. fischeri ascospores in pineapple and papaya nectars under the
conditions of temperature and production age of 25ºC for 1 month and using ratios of the heating medium of 10 for pineapple
nectar and 26 for papaya nectar. In these cases, the activation time was 5 minutes for the pineapple nectar and 10 minutes for the
papaya nectar at 85ºC. For the ascospores produced at 35ºC over three months, heated in pineapple nectar at a ratio of 38 and in...
Tipo: Journal article
Palavras-chave: Ascospores; Ratio; Neosartorya fischeri; Activation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300021
Influence of bacteriocins produced by Lactobacillus plantarum BN in the shelf-life of refrigerated
bovine meat
Provedor de dados: 58
Autores: Fiorentini,Ângela M.; Sant'Anna,Ernani S.; Porto,Anna C.S.; Mazo,Jaciara Z.; Franco,Bernadette D.G.M.
Sugar cane molasses is a cheap by-product of the sugar cane industry. This product was used for growth and production of
bacteriocins by Lactobacillus plantarum BN and evaluated for its potential application in the extension of the shelf-life of raw
meat. Bovine meat cubes were dipped in the filtered and neutralized supernatant of the fermented broth (Treatment A) and stored
at 5ºC. Counts of psychrotrophic and mesophilic aerobic microorganisms, pH determination and total acidity were performed on
meat cubes after 0, 3, 6, 9, 12 and 15 days. These determinations were also done in cubes dipped in a 6% lactic acid solution
(treatment B) and distilled water (treatment C). After 3 days, the counts of psychrotrophic microorganisms in cubes submitted to...
Tipo: Journal article
Palavras-chave: Lactobacillus plantarum BN; Bacteriocins; Bovine meat; Shelf-life.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100010
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review
Provedor de dados: 58
Autores: Valones,Marcela Agne Alves; Guimarães,Rafael Lima; Brandão,Lucas André Cavalcanti; Souza,Paulo Roberto
Eleutério de; Carvalho,Alessandra de Albuquerque Tavares; Crovela,Sergio.
Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad
range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these
methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an
excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR
techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical
diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is
considered a fast and accurate...
Tipo: Journal article
Palavras-chave: Conventional PCR; Real-Time PCR; Molecular Biology; Methods of molecular detection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001
Synergistic effects of tacrolimus and azole antifungal compounds in fluconazole-susceptible and
fluconazole-resistant Candida glabrata isolates
Provedor de dados: 58
Autores: Denardi,Laura Bedin; Mario,Débora Alves Nunes; Loreto,Érico Silva; Santurio,Janio Morais; Alves,Sydney Hartz.
In vitro interaction between tacrolimus (FK506) and four azoles (fluconazole, ketoconazole, itraconazole and voriconazole)
against thirty clinical isolates of both fluconazole susceptible and -resistant Candida glabrata were evaluated by the checkerboard
microdilution method. Synergistic, indifferent or antagonism interactions were found for combinations of the antifungal agents
and FK506. A larger synergistic effect was observed for the combinations of FK506 with itraconazole and voriconazole (43%),
followed by that of the combination with ketoconazole (37%), against fluconazole-susceptible isolates. For fluconazole-resistant
C. glabrata, a higher synergistic effect was obtained from FK506 combined with ketoconazole (77%), itraconazole (73%),
voriconazole...
Tipo: Journal article
Palavras-chave: Candida glabrata; Azole resistance; FK506; Combination therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100125
Vegetative compatibility and heterokaryon formation between different isolates of Colletotrichum
lindemuthianum by using the nit mutant system
Provedor de dados: 58
Autores: Carvalho,Camila Rodrigues de; Mendes-Costa,Maria Cristina.
Colletotrichum lindemuthianum, the causative agent of bean anthracnose, is one of the most common pathogens leading to
expressive damage to plants beyond presenting noticeable variability. The knowledge on vegetative compatibility groups (VCGs)
is of particular interest in asexual fungi as they subdivide the population in groups that can exchange genetic information via
heterokaryosis and the parasexual cycle. Among the techniques used in studies about vegetative compatibility groups, the
obtainment of nit mutants is apparent. This paper is aimed at obtaining heterokaryons between different isolates of C.
lindemuthianum, grouping them in VCGs and evaluating their genetic variability by using the nit mutants system. Nit mutants
were obtained from 20 single...
Tipo: Journal article
Palavras-chave: Colletotrichum lindemuthianum; Anthracnose; Heterokaryosis; Nit mutants; VCG.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100044
Aspergillosis: a limiting factor during recovery of captive magellanic penguins
Provedor de dados: 58
Autores: Xavier,Melissa O.; Soares,Mauro P.; Meinerz,Ana Raquel M.; Nobre,Márcia O.; Osório,Luiza G.; Silva Filho,Rodolfo
P. da; Meireles,Mário Carlos A.
The article describes the epidemiology, macroscopic and histological lesions as well as the isolation of Aspergillus flavus and A.
fumigatus from Magellanic penguins (Spheniscus magellanicus) during recovery in the Center for Recovery of Marine Animals
(CRAM - 32ºS/52ºW), over a period of two years. From January 2004 to December 2005 the Center received 52 Magellanic
penguins, and 23% (12/52) died. Necropsies were performed and tissue samples were collected for histological and
microbiological examination. From 12 dead animals, aspergillosis was confirmed in five animals, corresponding to 42% of the
mortality. Granulomatous nodules were observed mainly on air sacs and lungs. Histologically, septate and branching hyphae,
measuring 3-5 µm and PAS positive...
Tipo: Journal article
Palavras-chave: Aspergillosis; Rehabilitation; Penguins; Captivity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300018
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils
Provedor de dados: 58
Autores: Millezi,Alessandra Farias; Cardoso,Maria das Graças; Alves,Eduardo; Piccoli,Roberta Hilsdorf.
This study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or
reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the
reduction effect of sanitizing detergents containing essential oils of Thymus vulgaris (thyme) and Cymbopogon citratus
(lemongrass) on biofilm formed by A. hydrophila on AISI 304 stainless steel coupons, using UHT skimmed milk as substratum.
There was adhesion and biofilm formation by A. hydrophila at 28 ºC, presenting 7.60 log cfu.cm-2 after the fourth day of
cultivation. There was no significant difference between the lemongrass treatment and that of the thyme oil (p < 0.05).
However, both treatments...
Tipo: Journal article
Palavras-chave: Thymus vulgaris; Cymbopogon citratus; Natural disinfectants; Sanitation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100010
Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for
fast molecular identification and genotyping
Provedor de dados: 58
Autores: Miyata,Marcelo; Santos,Adolfo Carlos Barreto; Mendes,Natália Helena; Cunha,Eunice Atsuko; Melo,Fernando Augusto
Fiúza de; Leite,Clarice Queico Fujimura.
We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular
identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that
with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
Tipo: Journal article
Palavras-chave: DNA extraction; Mycobacterium tuberculosis; Thermolysis; CTAB.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
Production of humic substances through coal-solubilizing bacteria
Provedor de dados: 58
Autores: Valero,Nelson; Gómez,Liliana; Pantoja,Manuel; Ramírez,Ramiro.
In this paper, the production of humic substances (HS) through the bacterial solubilization of low rank coal (LRC) was evaluated.
The evaluation was carried out by 19 bacterial strains isolated in microenvironments with high contents of coal wastes. The
biotransformed LRC and the HS produced were quantified in vitro in a liquid growth medium. The humic acids (HA) obtained
from the most active bacterial strain were characterized via elemental composition (C, H, N, O), IR analyses, and the E4/E6 ratio;
they were then compared with the HA extracted chemically using NaOH. There was LRC biotransformation ranged from 25 to
37%, and HS production ranged from 127 to 3100 mg.L-1. More activity was detected in the isolated strains of Bacillus
mycoides,...
Tipo: Journal article
Palavras-chave: Low rank coal; Microbacterium sp; Bacillus mycoides; Acinetobacter baumannii; Enterobacter aerogenes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300021
Detection of trypsin inhibitor in seeds of Eucalyptus urophylla and its influence on the in vitro growth
of the fungi Pisolithus tinctorius and Rhizoctonia solani
Provedor de dados: 58
Autores: Tremacoldi,Célia Regina; Pascholati,Sérgio Florentino.
Inhibitors of plant proteases can regulate the hydrolysis of proteins inside the cells and also participate in the mechanisms of plant
defense against herbivore insects and pathogens. Here, we demonstrated that seeds of Eucalyptus urophylla exhibit activities of
trypsin and papain inhibitors, two proteases commonly found in living cells. Low amounts of proteins of the crude protein extract
of seeds and fractions partially purified by gel filtration, with inhibitory activity against trypsin, inhibited in vitro the mycelial
growth of a compatible isolate of the ectomycorrhizal fungus Pisolithus tinctorius and allowed an unsatisfactory growth of another
isolate from Pinus taeda, considered incompatible for this eucalyptus species. The same amounts of...
Tipo: Journal article
Palavras-chave: Protease; Inhibitor; Trypsin; Pisolithus tinctorius; Rhizoctonia solani.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400001
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric
Fever
Provedor de dados: 58
Autores: Cury,Gisele Cristiane Gentile; Pereira,Rafaella Fabiana Carneiro; Hollanda,Luciana Maria de; Lancellotti,Marcelo.
The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually
preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated
exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were
fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae
strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells
line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular
study...
Tipo: Journal article
Palavras-chave: Brazilian purpuric fever; Haemophilus influenzae biogroup aegyptius; Genetic transfer; Neisseria meningitidis;
Haemophilus influenzae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040
Antimicrobial resistance and serotyping of Streptococcus pneumoniae isolated from pediatric patients
in Belo Horizonte, MG, Brazil
Provedor de dados: 58
Autores: Magalhães,Ana Paula Gomes de Oliveira; Pinto,Artur da Silveira.
Thirty one Streptococcus pneumoniae invasive strains were isolated from a pediatric population in Belo Horizonte from June,
1999 to May, 2001. Penicillin, trimethoprim-sulfamethoxazole, tetracycline and chloramphenicol resistance rates for the isolates
were 41.9, 58.1, 25.8 and 3.2%, respectively. Intermediate penicillin resistant (MICs between 0.1 and 1.0 µg/ml) and resistant
(MICs > 2.0 µg/ml) isolates occured at rates of 38.7 and 3.2%, respectively. Resistance to erythromycin, ofloxacin, rifampin or
vancomicyn was not detected. Ten S. pneumoniae serotypes (14, 5, 10 A, 6B, 15B, 18C, 6 A, 18 A, 19 A and 19 F) were
identified. Serotype 14 (12 out of 31) was predominant among the isolates. Penicillin and trimethoprim-sulfamethoxazole
resistance was more...
Tipo: Journal article
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotyping.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300005
Antifungal susceptibility of clinical and environmental Cryptococcus neoformans and Cryptococcus
gattii isolates in Jabalpur, a city of Madhya Pradesh in Central India
Provedor de dados: 58
Autores: Gutch,Ruchi Sethi; Nawange,Shesh Rao; Singh,Shankar Mohan; Yadu,Ruchika; Tiwari,Aditi; Gumasta,Richa;
Kavishwar,Arvind.
In this study, we present antifungal susceptibility data of clinical and environmental isolates of Central Indian Cryptococcus
neoformans (Serotype A, n = 8 and n = 50 respectively) and Cryptococcus gattii (Serotype B, n = 01 and n = 04 respectively).
Susceptibilities to fluconazole, itraconazole and ketoconazole were determined by using NCCLS broth micro-dilution
methodology. The total number of resistant strains for fluconazole in case of C. neoformans and C. gattii showed a significant
difference by using chi-square test (p < 0.05*), while considering fisher's exact p value was nonsignificant (p > 0.05).
However, the total number of resistant strains for itraconazole and ketoconazole was not found statistically significant. A
comparison of...
Tipo: Journal article
Palavras-chave: Cryptococcus neoformans; Cryptococcus gattii; Minimum inhibitory concentration; Azoles; Central India.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401125
Dermatophytosis caused by Microsporum canis and Microsporum gypseum in free-living Bradypus
variegatus (Schiz, 1825) in the state of Pernambuco, Brazil
Provedor de dados: 58
Autores: Xavier,Gileno Antônio Araújo; Silva,Leonildo Bento Galiza da; Silva,Davi Rubem da; Peixoto,Rodolfo de Moraes;
Lino,Gileno Câmara; Mota,Rinaldo Aparecido.
Three cases of dermatophytosis in free living brown-throated three-toed sloths (Bradypus variegatus) in the Zona da Mata, North
of Pernambuco State, Brazil, were studied. Two animals presented areas of alopecia on the pelvic member and thorax and one
animal on the pelvic member only. The three animals presented scabs. Hair and scabs samples were submitted to microscopical
examination after treatment with a 30 % KOH and cultivated in Mycosel Agar. The direct examination indicated the presence of
arthrospores in the hair. Colonies grown after seven days of culture were confirmed as Microsporum based on examination of the
structure of the macroconidia. This is the first observation of dermatophytosis caused by Microsporum canis and Microsporum
gypseum in free...
Tipo: Journal article
Palavras-chave: Microsporum canis; Microsporum gypseum; Bradypus variegatus; Dermatophytosis; Sloths.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300018
Use of P450 cytochrome inhibitors in studies of enokipodin biosynthesis
Provedor de dados: 58
Autores: Ishikawa,Noemia Kazue; Tahara,Satoshi; Namatame,Tomohiro; Farooq,Afgan; Fukushi,Yukiharu.
Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes,
an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D
attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic
intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three
less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and
epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a
new compound.
Tipo: Journal article
Palavras-chave: Antimicrobial compound cuparene-1; 4-quinone edible mushroom enokitake Flammulina velutipes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400037
Live bacterial vaccine vectors: An overview
Provedor de dados: 58
Autores: Silva,Adilson José da; Zangirolami,Teresa Cristina; Novo-Mansur,Maria Teresa Marques; Giordano,Roberto de
Campos; Martins,Elizabeth Angélica Leme.
Genetically attenuated microorganisms, pathogens, and some commensal bacteria can be engineered to deliver recombinant
heterologous antigens to stimulate the host immune system, while still offering good levels of safety. A key feature of these live
vectors is their capacity to stimulate mucosal as well as humoral and/or cellular systemic immunity. This enables the use of
different forms of vaccination to prevent pathogen colonization of mucosal tissues, the front door for many infectious agents.
Furthermore, delivery of DNA vaccines and immune system stimulatory molecules, such as cytokines, can be achieved using
these special carriers, whose adjuvant properties and, sometimes, invasive capacities enhance the immune response. More
recently, the unique...
Tipo: Journal article
Palavras-chave: Bacterial vector; Vaccine delivery system; DNA vaccine; Cancer vaccine; Antigen presentation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400001
Campylobacter jejuni in commercial eggs
Provedor de dados: 58
Autores: Fonseca,Belchiolina Beatriz; Beletti,Marcelo Emílio; Melo,Roberta Torres de; Mendonça,Eliane Pereira; Coelho,Letícia
Ríspoli; Nalevaiko,Priscila Christen; Rossi,Daise Aparecida.
This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and
colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the
survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C.
jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the
viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we
used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after...
Tipo: Journal article
Palavras-chave: Campylobacteriosis; Commercial eggs; Penetration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100011
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through
nested-PCR
Provedor de dados: 58
Autores: Araújo,Cristina P.; Osório,Ana Luiza A.R.; Jorge,Klaudia S.G.; Ramos,Carlos A.N.; Souza Filho,Antonio F.;
Vidal,Carlos E.S.; Vargas,Agueda P.C.; Roxo,Eliana; Rocha,Adalgiza S.; Suffys,Philip N.; Fonseca Júnior,Antônio A.;
Silva,Marcio R.; Barbosa Neto,José D.; Cerqueira,Valíria D.; Araújo,Flábio R.
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine
tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable
results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with
conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms
directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA
samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA
samples...
Tipo: Journal article
Palavras-chave: Bovine and bubaline tuberculosis; Nested-PCR; Real-time PCR; Tissue; Sanitary inspection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200035
Genetic diversity of the VP1/VP2 gene of canine parvovirus type 2b amplified from clinical specimens
in Brazil
Provedor de dados: 58
Autores: Pereira,Cesar A. D.; Durigon,Edison Luiz.
We evaluated the genetic diversity in the VP1/VP2 gene of CPV type 2b isolates from symptomatic dogs in Brazil. A total of 21
isolates collected from 1990 through 1995 previously typed as CPV2b by PCR assay were studied. Overall we found a high
degree of similarity among sequences from different CPV clinical isolates collected. Genetic analysis of this selected region gave
no indication of a specific Brazilian parvovirus lineage.
Tipo: Journal article
Palavras-chave: Genetic diversity; Canine parvovirus (CPV); VP1/VP2 gene.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400014
Biocontrol potential of salinity tolerant mutants of Trichoderma harzianum against Fusarium
oxysporum
Provedor de dados: 58
Autores: Mohamed,Hassan Abdel-Latif A.; Haggag,Wafaa Mohamed.
Exposing a wild-type culture of Trichoderma harzianum to gamma irradiation induced two stable salt-tolerant mutants (Th50M6
and Th50M11). Under saline conditions, both mutants greatly surpassed their wild type strain in growth rate, sporulation and
biological proficiency against Fusarium oxysporum, the causal agent of tomato wilt disease. Tolerant T. harzianum mutants
detained a capability to grow and convinced sporulation in growth media containing up to 69 mM NaCl. In comparison with their
parent strain, characterization of both mutants confirmed that they have reinforced contents of proline and hydroxyproline,
relatively higher sodium content compared to potassium, calcium or magnesium contents, higher level of total phenols.
Electrophoretic analysis of...
Tipo: Journal article
Palavras-chave: Antifungal metabolites; Mutagenesis; Gamma-irradiation; Salt stress; Trichoderma harzianum; Fusarium
oxysporium.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200016
Contagious agalactia by Mycoplasma agalactiae in small ruminants in Brazil: first report
Provedor de dados: 58
Autores: Azevedo,Edisio Oliveira de; Alcântara,Maria Dalva Bezerra de; Nascimento,Elmiro Rosendo do; Tabosa,Ivon Macedo;
Barreto,Maria Lúcia; Almeida,Juliana Ferreira de; Araújo,Marília D'Oliveira; Rodrigues,Ana Rosa Oliveira; Riet-Correa,Franklin;
Castro,Roberto Soares de.
Two outbreaks of contagious agalactia by Mycoplasma agalactiae occurred in Paraíba State, Northeastern Region of Brazil are
reported. The disease was characterized by mastitis, agalactia and polyarthritis in does and polyarthritis and conjunctivitis in kids
and lambs. Fever and anorexia were also observed. Morbidy was from 26.1% to 100% in does, 36.5 to 100% in kids and 49% in
lambs. In one farm 14.3% of the lactating goats and 6.4% of the kids died or were euthanized. In the other, 3.3% of the does,
36.5% of the kids and 22.9% of the lambs died and 84 affected goats were euthanized to control the disease. M. agalactiae was
isolated from milk, joint exudates, nasal swabs and ear washings. The colonies were characteristic of Mycoplasma and the agent
did not...
Tipo: Journal article
Palavras-chave: Contagious agalactia; Mycoplasma agalactiae; Small ruminants.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400033
Gastric Helicobacter spp. infection in captive neotropical Brazilian feline
Provedor de dados: 58
Autores: Camargo,Pedro Luiz de; Uenaka,Simone Akemi; Motta,Maitê Bette; Adania,Cristina Harumi; Yamasaki,Letícia;
Alfieri,Amauri A; Bracarense,Ana Paula F. R. L.
Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus,
corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay
with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions
were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter
heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was
observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant
association...
Tipo: Journal article
Palavras-chave: Helicobacter; Feline; Gastric diseases; PCR; Leopardus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100037
Application of response surface methodology to enhancement of biomass production by Lactobacillus
rhamnosus E/N
Provedor de dados: 58
Autores: Polak-Berecka,Magdalena; Waśko,Adam; Kordowska-Wiater,Monika;
Targoński,Zdzisław; Kubik-Komar,Agnieszka.
Response surface methodology (RSM) was employed to study the effects of various medium components on biomass production
by Lactobacillus rhamnosus E/N. This strain is commonly used in the pharmaceutical and food industries due to its beneficial
effect on the human gut and general health. The best medium composition derived from RSM regression was (in g/l) glucose
15.44, sodium pyruvate 3.92, meat extract 8.0, potassium phosphate 1.88, sodium acetate 4.7, and ammonium citrate 1.88. With
this medium composition biomass production was 23 g/l of dry cell weight after 18 h of cultivation in bioreactor conditions,
whereas on MRS the yield of biomass was 21 g/l of dry cell weight. The cost of 1 g of biomass obtained on MRS broth was
calculated at the level of...
Tipo: Journal article
Palavras-chave: Response surface methodology; Lactobacillus rhamnosus; Medium selection; Biomass production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400035
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical
approach
Provedor de dados: 58
Autores: Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M.
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158
grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the
Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further
optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors
(soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found
to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
Tipo: Journal article
Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design
(CCD).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil
Provedor de dados: 58
Autores: Morillo,Simone Guadagnucci; Audrey,Cilli; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do Carmo
Sampaio Tavares.
Norovirus (NoV), previously called Norwalk-like virus, represents an important group of human pathogens associated with
outbreaks of nonbacterial gastroenteritis. Epidemiological surveys of outbreaks have shown that the most important routes of
transmission are person-to-person contacts and contaminated food and water, with the virus affecting adults and children. NoV is
classified into genogroups, being genogroups GI, GII and GIV found in humans. In view of the high genetic diversity of NoV and
the lack of information about this virus in Brazil, the aim of the present study was the molecular characterization of NoV isolated
from diarrheic stool samples of patients from São Paulo State. In this study, 204 stool specimens collected during diarrhea
outbreaks...
Tipo: Journal article
Palavras-chave: Norovirus; Genogroup GII; Molecular characterization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400004
Production of laccase by Pynoporus sanguineus using 2,5 - Xylidine and ethanol
Provedor de dados: 58
Autores: Valeriano,Viviane S.; Silva,Anna Maria F.; Santiago,Mariângela F.; Bara,Maria T. F.; Garcia,Telma A.
Enzyme application in biotechnological and environmental processes has had increasing interest due to its efficiency, selectivity
and mainly for being environmentally healthful, but these applications require a great volume of enzymes. In this work the effect
of different concentrations of ethanol and 2,5 - xylidine on growth and production of laccase by Pycnoporus sanguineus was
investigated. In a medium containing 200 mg.L-1 of 2,5 - xylidine or 50 g.L-1 of ethanol, the maximum activity of laccase was
2019 U.L-1 and 1035 U.L-1, respectively. No direct correlation between biomass and activity of laccase was observed for any of
the inducers used during the tests. Ethanol concentrations, larger than or equal to 20 g.L-1, inhibited the radial growth of P....
Tipo: Journal article
Palavras-chave: Laccase Pycnoporus sanguineus ethanol 2; 5-xylidine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400009
Nosocomial outbreaks due to Pseudomonas aeruginosa and Acinetobacter baumannii in a Neonatal
Intensive Care Unit (NICU) of the Uberlândia Federal University Hospital
Provedor de dados: 58
Autores: Brito,Denise Von Dolinger de; Oliveira,Elias Jose; Darini,Ana Lúcia da Costa; Abdallah,Vânia Olivetti Sttephen;
Gontijo-Filho,Paulo Pinto.
The study documents the occurrence of two subsequent outbreaks in the NICU of HC-UFU, caused by epidemic strains of
multiresistant Pseudomonas aeruginosa and Acinetobacter baumannii, that occurred between March/01 and September and
between October/01 and March/02, respectively. The P. aeruginosa outbreak included seven neonates with conjunctivitis and three
with bacteremia. A case-control study was conducted for the A. baumannii outbreak, with 11 and 22 neonates, respectively. The
isolates of A. baumannii were resistant to gentamacin and ciprofloxacin. P. aeruginosa isolates were resistant to
ampicillin/sulbactam gentamicin and ciprofloxacin. The hands of healthcare workers and environmental cultures were negative.
The outbreak of P. aeruginosa resulted in...
Tipo: Journal article
Palavras-chave: Outbreak; Neonate; Acinetobacter baumannii; Pseudomonas aeruginosa.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500009
Assessing the sources of high fecal coliform levels at an urban tropical beach
Provedor de dados: 58
Autores: Davino,Aline Mendonça Cavalcante; Melo,Milena Bandeira de; Caffaro Filho,Roberto Augusto.
Recreational water quality is commonly assessed by microbial indicators such as fecal coliforms. Maceió is the capital of Alagoas
state, located in tropical northeastern Brazil. Its beaches are considered as the most beautiful urban beaches in the country. Jatiúca
Beach in Maceió was found to be unsuitable for bathing continuously during the year of 2011. The same level of contamination
was not observed in surrounding beaches. The aim of this study was to initiate the search for the sources of these high coliform
levels, so that contamination can be eventually mitigated. We performed a retrospective analysis of historical results of fecal
coliform concentrations from 2006 to 2012 at five monitoring stations located in the study region. Results showed that...
Tipo: Journal article
Palavras-chave: Recreational water quality; Fecal indicators; Coastal pollution.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401019
Comparison of different selective enrichment steps to isolate Salmonella sp. from feces of finishing
swine
Provedor de dados: 58
Autores: Michael,Geovana Brenner; Simoneti,Roselis; Costa,Marisa da; Cardoso,Marisa.
A two-phase study was conducted to compare the efficacy of several enrichment selective-broth steps associated to different
plating media for recovery of Salmonella sp. from finishing swine feces. In a first phase, Rappaport-Vassiliadis broth (RV)
incubated at 42ºC, Tetrathionate Müller-Kauffmann broth at 37ºC (TMK37) and 42ºC (TMK42), and Selenite Cystine broth (SC)
at 37ºC, in combination with three selective plating media Rambach agar (RA), Xylose-Lysine-Tergitol 4 agar (XLT4), and
Brilliant-Green Phenol-Red Lactose Sucrose agar (VB) were compared for recovery of Salmonella from artificially contaminated
swine feces. In a second phase, RV, TMK37, and TMK42, associated with XLT4 and VB , were tested with naturally
contaminated swine feces. In this study...
Tipo: Journal article
Palavras-chave: Salmonella sp.; Selective enrichment; Swine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200009
DesinFix TM 135 in fermentation process for bioethanol production
Provedor de dados: 58
Autores: Barth,Dorothee; Monteiro,Ana Raquel de Souza; Costa,Marcelo Moreira da; Virkajärvi,Ilkka; Sacon,Vera;
Wilhelmsom,Annika.
Brazil has the world's largest ethanol production from sugarcane, but bacterial contamination decreases the ethanol yields. It was
shown that the biocide DesinFixTM 135 can reduce the contamination without decreasing the yeasts' viability or negatively
affecting the ethanol production.
Tipo: Journal article
Palavras-chave: Ethanol production; Fermentation process; Sugar cane feedstock; Biocides; Bioethanol.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100046
Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method
Provedor de dados: 58
Autores: Abdullah,J.; Saffie,N.; Sjasri,F.A.R.; Husin,A.; Abdul-Rahman,Z.; Ismail,A.; Aziah,I.; Mohamed,M.
An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity
in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two
outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was
optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes
of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further
evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold
standard culture...
Tipo: Journal article
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Salmonella Typhi.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400032
Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary
symptoms
Provedor de dados: 58
Autores: Zarrinfar,Hossein; Kaboli,Saeed; Dolatabadi,Somayeh; Mohammadi,Rasoul.
Abstract Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one
of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic
consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important
information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar
lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were
identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain
reaction–restriction...
Tipo: Journal article
Palavras-chave: Candida; Pulmonary; PCR-RFLP.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100172
Epidemiologically relevant antimicrobial resistance phenotypes in pathogens isolated from critically
ill patients in a Brazilian Universitary Hospital
Provedor de dados: 58
Autores: Carvalho,Rodolfo Henriques de; Gontijo Filho,Paulo P.
Antimicrobial resistance is a threat to public health worldwide and is associated with higher mortality and morbidity. Despite the
extensive knowledge about this problem, drug resistance has continued to emerge, especially in intensive care units (ICUs). The
objective of this study was to evaluate the frequencies of epidemiologically relevant resistance phenotypes in pathogens isolated
from ventilator-associated pneumonia (VAP), bloodstream infections (BSI) and urinary tract infections (UTI) in patients admitted
in the adult intensive care unit (AICU) of the Clinical Hospital of Federal University of Uberlândia, during an one year period.
Additionally, at the period of the study, the antibiotic consumption in AICU was verified. Coagulase-negative...
Tipo: Journal article
Palavras-chave: Nosocomial infection; Epidemiology; Multiresistant microorganisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400005
Antifungal suscepitibility profile of candida spp. oral isolates obtained from denture wearers
Provedor de dados: 58
Autores: Lyon,J.P.; Moreira,L.M.; Cardoso,M.A.G.; Saade,J.; Resende,M.A.
Denture stomatitis is an inflammatory condition that occurs in denture wearers and is frequently associated with Candida yeasts.
Antifungal susceptibility profiles have been extensively evaluated for candidiasis patients or immunosupressed individuals, but
not for healthy Candida carriers. In the present study, fluconazole, itraconazole, voriconazole, terbinafine and 5-flucytosin were
tested against 109 oral Candida spp. isolates. All antifungal agents were effective against the samples tested except for terbinafine.
This work might provide epidemiological information about Candida spp. drug susceptibility in oral healthy individuals.
Tipo: Journal article
Palavras-chave: Denture wearers; Candida carriage; Normal oral mucosa; Antifungal agents.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400013
Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures
and concentrations of sodium chloride
Provedor de dados: 58
Autores: Lee,H.Y.; Chai,L.C.; Pui,C.F.; Mustafa,S.; Cheah,Y.K.; Nishibuchi,M.; Radu,S.
Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns.
Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and
its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L.
monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1-10%
(w/v) and incubated at different temperature of 4 ºC, 30 ºC and 45 ºC for up to 60 h. Absorbance reading of crystal violet staining
showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4 ºC. The
formation of biofilm...
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Biofilm; Sodium chloride; Temperature; Microtiter plate.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100007
In vitro synergistic activity of antibiotic combinations against Brucella melitensis using E-test
methodology
Provedor de dados: 58
Autores: Kilic,Selcuk; Dizbay,Murat; Hizel,Kenan; Arman,Dilek.
The treatment of brucellosis is still problematic, because of high rates of treatment failure or relapses. As the microorganism is an
intracellular pathogen, treatment requires combined regimens. However, limited existing data on in vitro combinations are
avaliable for Brucellae. The aim of this study was to investigate the in vitro efficacy of various traditional and new antibiotic
combinations against 16 Brucella melitensis strains. The combination effect of antimicrobial agents was evaluated by E-test
synergy method to obtain a fractional inhibitory concentration (FIC) index. Co-Trimoxazole (SXT) and moxifloxocin (MXF)
exhibited the lowest MIC, while Rifampin (RIF) had the highest MIC in the study. Combinations with RIF showed the best
synergistic...
Tipo: Journal article
Palavras-chave: Brucella melitensis; Antibiotics; Synergism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200006
Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis
Provedor de dados: 58
Autores: Fornari,Gheniffer; Vicente,Vania Aparecida; Gomes,Renata Rodrigues; Muro,Marisol Dominguez; Pinheiro,Rosangela
Lameira; Ferrari,Carolina; Herkert,Patricia Fernanda; Takimura,Marcos; Carvalho,Newton Sérgio de; Queiroz-Telles,Flavio.
Abstract Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15–25% of
vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of
clinical symptoms. The isolates were subjected to in vitro susceptibility profile and characterization by molecular markers, which
intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar,
API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicans strains were genotyped by the ABC system
and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyr
and...
Tipo: Journal article
Palavras-chave: Vulvovaginal candidiasis; In vitro susceptibility; Variability genetic.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200373
Ochrobactrum anthropi bacteremia in a preterm infant with cystic fibrosis
Provedor de dados: 58
Autores: Menezes,Fernando Gatti; Abreu,Maria Gabriela Ballalai; Kawagoe,Julia Yaeko; Warth,Arno Norberto; Deutsch,Alice
D'Agostini; Dornaus,Maria Fernanda P S; Martino,Marines Dalla Valle; Correa,Luci.
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and
unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report
the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Tipo: Journal article
Palavras-chave: Bacteremia; Cystic fibrosis; Newborn; Meconium; Premature birth.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200026
Use of pcr-rflp of thefla a gene for detection and subtyping of Campylobacter jejuni strains
Potentially related to Guillain-barré syndrome, isolated from humans and animals
Provedor de dados: 58
Autores: Scarcelli,E.; Piatti,R.M.; Harakava,R.; Miyashiro,S.; Campos,F.R.; Souza,M.C.A.; Cardoso,M.V.; Teixeira,S.R.;
Genovez,M.E.
The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and
different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN
O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human
feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses
(n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction
endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I;
and seven when...
Tipo: Journal article
Palavras-chave: Campylobacter jejuni; PCR-RFLP; Subtyping; Guillain-Barré Syndrome.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029
Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their
potential PGPR characteristics
Provedor de dados: 58
Autores: Calvo,Pamela; Ormeño-Orrillo,Ernesto; Martínez-Romero,Esperanza; Zúñiga,Doris.
Bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (PGP) activities that
include biocontrol capacity against some phytopatogenic fungi. Potato crops in the Andean Highlands of Peru face many
nutritional and phytophatogenic problems that have a significant impact on production. In this context is important to investigate
the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising PGP
strains. In this study, sixty three Bacillus strains isolated from the rhizosphere of native potato varieties growing in the Andean
highlands of Peru were screened for in vitro antagonism against Rhizoctonia solani and Fusarium solani. A high prevalence (68%)
of...
Tipo: Journal article
Palavras-chave: Bacillus; Plant growth-promoting rhizobacteria; Potato; Antagonism; Phosphate solubilization; 16S rRNA gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400008
Aminotransferase and glutamate dehydrogenase activities in lactobacilli and streptococci
Provedor de dados: 58
Autores: Peralta,Guillermo Hugo; Bergamini,Carina Viviana; Hynes,Erica Rut.
ABSTRACT Aminotransferases and glutamate dehydrogenase are two main types of enzymes involved in the initial steps of
amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, glutamate dehydrogenase
and aminotransferase activities were screened in twenty one strains of lactic acid bacteria of dairy interest, either cheese-isolated
or commercial starters, including fifteen mesophilic lactobacilli, four thermophilic lactobacilli, and two streptococci. The strains
of Streptococcus thermophilus showed the highest glutamate dehydrogenase activity, which was significantly elevated compared
with the lactobacilli. Aspartate aminotransferase prevailed in most strains tested, while the levels and specificity of other...
Tipo: Journal article
Palavras-chave: Aminotransferase activity; Glutamate dehydrogenase activity; Lactobacilli; Streptococci.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300741
Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in
Portugal
Provedor de dados: 58
Autores: Ragonezi,Carla; Caldeira,A. Teresa; Rosário Martins,M.; Salvador,Cátia; Santos-Silva,Celeste; Ganhão,Elsa;
Klimaszewska,Krystyna; Zavattieri,Amely.
Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in
natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM
sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during
acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure
cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro
co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes.
To...
Tipo: Journal article
Palavras-chave: Pisolithus arhizus; Lactarius deliciosus; Pinus pinea; M13-PCR; ITS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047
Emergence of VanB phenotype-vanA genotype in vancomycin-resistant enterococci in Brazilian
hospital
Provedor de dados: 58
Autores: Zanella,Rosemeire Cobo; Lima,Marisa de Jesus Castro; Tegani,Luciana Soares; Hitomi,Adriana; Brandileone,Maria
Cristina de Cunto; Palazzo,Izabel Cristina V.; Darini,Ana Lúcia da Costa.
In Brazil, vancomycin-resistant enterococci (VRE) have been reported as nosocomial pathogens since 1998. Recently, in a VRE
surveillance in a hospital, we detected two Enterococcus faecalis isolates with vanA genotype and susceptible to teicoplanin. This
is the first report of VanB phenotype-vanA genotype enterococci isolated from humans in Brazil.
Tipo: Journal article
Palavras-chave: Enterococci; VanA genotype; VanB phenotype; Vancomycin; VRE.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200004
Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica
Provedor de dados: 58
Autores: Cotârleţ,Mihaela; Negoiţă,Teodor Gh; Bahrim,Gabriela E; Stougaard,Peter.
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to
characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and
to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that
the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands.
The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased
after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while
alpha-amylase and...
Tipo: Journal article
Palavras-chave: Streptomyces sp; East Antarctica; Cold-adapted strain; Cold active proteases and amylase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005
Feather degradation by strains of Bacillus isolated from decomposing feathers
Provedor de dados: 58
Autores: Swetlana,Nagal; Jain,P. C.
Feather waste is generated in large amounts as a by-product of commercial poultry processing. This residue is almost pure keratin,
which is not easily degradable by common proteolytic enzymes. Eight strains of Bacillus, isolated from decomposing feathers
were tested for the hydrolysis of feather wastes in the laboratory. Among these strains, Bacillus cereus KB043 was the best feather
degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. It caused
78.16 ± 0.4 % degradation with a significant release of soluble protein (1206.15 ± 14.7 µg mL-1) and cysteine (20.63 ± 0.4 µg
mL-1) in the cultivation fluid. The strain also showed the highest level of keratinase activity (39.10 ± 0.4 U mL-1). These data...
Tipo: Journal article
Palavras-chave: Bacillus; Feather degradation; Poultry waste; Soluble protein; Keratinase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100028
Biofilm formation on polystyrene under different temperatures by antibiotic resistant Enterococcus
faecalis and Enterococcus faecium isolated from food
Provedor de dados: 58
Autores: Marinho,A.R.; Martins,P.D.; Ditmer,E.M.; d'Azevedo,P.A.; Frazzon,J.; Sand,S.T.Van Der; Frazzon,A.P.G.
The ability of antibiotic resistant E. faecalis and E. faecium isolated from food to form biofilm at different temperatures in the
absence or presence of 0.75% glucose was evaluated. A synergistic effect on biofilm at 10 °C, 28 °C, 37 °C and 45 °C and glucose
was observed for E. faecalis and E. faecium.
Tipo: Journal article
Palavras-chave: Enterococci; Resistant; Food; Temperatures; Biofilm; Polystyrene.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200012
Phylogenetic analysis of Dengue virus 1 isolated from South Minas Gerais, Brazil
Provedor de dados: 58
Autores: Drumond,Betania Paiva; Fagundes,Luiz Gustavo da Silva; Rocha,Raissa Prado; Fumagalli,Marcilio Jorge; Araki,Carlos
Shigueru; Colombo,Tatiana Elisa; Nogueira,Mauricio Lacerda; Castilho,Thiago Elias; Silveira,Nelson José Freitas da;
Malaquias,Luiz Cosme Cotta; Coelho,Luiz Felipe Leomil.
Abstract Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world.
Primary infection with a single Dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. However, a
subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease,
called dengue hemorrhagic fever. The four Dengue virus serotypes (1–4) are antigenically and genetically distinct and
each serotype is composed of multiple genotypes. In this study we isolated one Dengue virus 1 serotype, named BR/Alfenas/2012,
from a patient with dengue hemorrhagic fever in Alfenas, South Minas Gerais, Brazil and molecular identification was...
Tipo: Journal article
Palavras-chave: Dengue virus-1; Alfenas; Minas Gerais; Phylogenetic analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100251
Nasal, oral and rectal microbiota of Black lion tamarins (Leontopithecus chrysopygus)
Provedor de dados: 58
Autores: Carvalho,Vania M.; Vanstreels,Ralph E.T.; Paula,Cátia D.; Kolesnikovas,Cristiane K.M.; Ramos,Maria Christina C.;
Coutinho,Selene D.; Martins,Cristiana S.; Pissinatti,Alcides; Catão-Dias,José L.
Black lion tamarins (Leontopithecus chrysopygus) are endangered callithrichids. Their conservation may require future
translocations or reintroductions; however these approaches involve risks of pathogen introduction in the environment and
stress-related opportunistic infections in these animals. In order to screen for opportunistic and potential pathogenic bacterial and
fungal microbiota, ten free-ranging and ten captive Black lion tamarins were studied and the results compared. Nasal, oral and
rectal swabs were collected and cultured for aerobic and facultative anaerobic bacteria and fungi, and a total 203 bacterial and 84
fungal isolates were obtained. Overall, the most frequent organisms were Staphylococcus spp., Bacillus spp., Candida spp. and...
Tipo: Journal article
Palavras-chave: Microbiota; Bacteria; Fungi; Black lion tamarins; Leontopithecus chrysopygus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400051
Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial
residue based solid-state cultivation
Provedor de dados: 58
Autores: Heck,Júlio X.; Hertz,Plinho F.; Ayub,Marco A.Z.
In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max) protein production. This material,
which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and
enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the
Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were
cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing
the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase,...
Tipo: Journal article
Palavras-chave: Solid-State cultivation; Cellulase; Xylanase; Soybean residue; Amazon biodiversity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300005
Antifungal activity of lectins against yeast of vaginal secretion
Provedor de dados: 58
Autores: Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson
Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina
Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they
have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according
to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its
fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of
leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out
in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Journal article
Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
Effect of gamma radiation on the inactivation of aflatoxin B1 in food and feed crops
Provedor de dados: 58
Autores: Ghanem,I.; Orfi,M.; Shamma,M.
Samples of food crops (peanut, peeled pistachio, unpeeled pistachio, rice, and corn) and feed (barley, bran, corn) were
autoclave-sterilized, and inoculated with 10(6) of spore suspension of an isolate of Aspergillus flavus fungus known to produce
aflatoxin B1 (AFB1) . Following a 10-day period of incubation at 27 C to allow for fungal growth, food and feed samples were
irradiated with gamma radiation at the doses 4, 6, and 10 kGy. Results indicated that degradation of AFB1 was positively
correlated with the increase in the applied dose of gamma ray for each tested sample. At a dose of 10 kGy percentages of AFB1
degradation reached highest values at 58.6, 68.8, 84.6, 81.1 and 87.8% for peanuts, peeled pistachios, unpeeled pistachios, corn
and rice samples,...
Tipo: Journal article
Palavras-chave: Aflatoxin B1; Food feed; Gamma radiation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400035
Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia
culture and protection against pathogens
Provedor de dados: 58
Autores: Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and
total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella
haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri
and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted
that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida
utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Journal article
Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017
Selection and characterization of carotenoid-producing yeasts from Campinas region, Brazil
Provedor de dados: 58
Autores: Maldonade,Iriani R.; Scamparini,Adilma R. P.; Rodriguez-Amaya,Délia B.
The objective of the present study was to select and identify yeasts from Brazil capable of producing carotenoids. Pigmented
yeasts were isolated from soil, leaves, fruits, flowers and a processed product. The samples were incubated at 30ºC in Erlenmeyer
flasks, containing YM broth. After 48 hours, they were inoculated in Petri dishes with YM agar, and incubated at 30ºC during 120
hours. The yeast colonies, which presented yellow to red coloration, were transferred to culture tubes containing YM agar, and
incubated at 30ºC for 72 hours. Out of 242 samples, only five had yellow to red color at high intensity. These highly pigmented
yeasts were re-isolated in Petri dishes with YM agar and then transferred to tubes with GPYM agar. Identification through...
Tipo: Journal article
Palavras-chave: Pigmented yeasts; Identification; Rhodotorula; Carotenoid; Fermentation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100014
Characterization of quinolone resistance in Salmonella spp. isolates from food products and human
samples in Brazil
Provedor de dados: 58
Autores: Pribul,Bruno Rocha; Festivo,Marcia Lima; Souza,Miliane Moreira Soares de; Rodrigues,Dalia dos Prazeres.
Abstract Non-typhoidal salmonellosis is an important zoonotic disease caused by Salmonella enterica. The aim of this study was
to investigate the prevalence of plasmid-mediated quinolone resistance in Salmonella spp. and its association with fluoroquinolone
susceptibility in Brazil. A total of 129 NTS isolates (samples from human origin, food from animal origin, environmental, and
animal) grouped as from animal (n = 62) and human (n = 67) food were evaluated between 2009 and 2013. These isolates were
investigated through serotyping, antimicrobial susceptibility testing, and the presence of plasmid-mediated quinolone resistance
(PMQR) genes (qnr, aac(6')-Ib) and associated integron genes (integrase, and conserved integron region). Resistance to
quinolones...
Tipo: Journal article
Palavras-chave: Foodborne diseases; Salmonella spp.; Quinolone resistance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100196
Cancer drugs inhibit morphogenesis in the human fungal pathogen, Candida albicans
Provedor de dados: 58
Autores: Routh,Madhushree M; Chauhan,Nitin M; Karuppayil,S Mohan.
Candida infections are very common in cancer patients and it is a common practice to prescribe antifungal antibiotics along with
anticancer drugs. Yeast to hyphal form switching is considered to be important in invasive candidiasis. Targeting morphogenetic
switching may be useful against invasive candidiasis. In this study, we report the antimorphogenetic properties of thirty cancer
drugs.
Tipo: Journal article
Palavras-chave: Candida albicans; Morphogenetic switching; Cancer drugs; Antifungal antibiotics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300029
Antibacterial activity of bacteriocin-like substance P34 on Listeria monocytogenes in chicken sausage
Provedor de dados: 58
Autores: Sant'Anna,Voltaire; Quadros,Deoni A.F.; Motta,Amanda S.; Brandelli,Adriano.
The antimicrobial activity of the bacteriocin-like substance (BLS) P34 against Listeria monocytogenes was investigated in
chicken sausage. The BLS was applied to chicken sausages (256 AU g-1) previously inoculated with a suspension of 10² cfu g-1
of L. monocytogenes. BLS P34 inhibited the indicator microorganism in situ in all incubation times for up to 10 days at 5 °C. The
effectiveness of BLS P34 was increased when it was added in combination with nisin. The bacteriocin was also tested in natural
eatable natural bovine wrapping (salty semi-dried tripe) against the same indicator microorganism, also showing inhibitory
capability in vitro. BLS P34 showed potential to control L. monocytogenes in refrigerated meat products.
Tipo: Journal article
Palavras-chave: Bacteriocin; Chilled chicken sausage; Listeria monocytogenes; Biopreservation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400019
Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with
Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki
Provedor de dados: 58
Autores: Knaak,Neiva; Fiuza,Lidia Mariana.
The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase
in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar
kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest
insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode
of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae
digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and...
Tipo: Journal article
Palavras-chave: Virus; Bacteria; Biological control; Lepidoptera.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200017
Growth of enterotoxin producing Bacillus cereus in meat substrate at 10ºC and 30ºC
Provedor de dados: 58
Autores: Soares,Celina Mara; Kabuki,Dirce Yorika; Kuaye,Arnaldo Yoshiteru.
The behaviour of enterotoxin-producing Bacillus cereus in meat was investigated by inoculating spore suspensions of five cultures
into meat substrate (pH 5.8) and incubating at 10ºC and 30ºC. The bacterial populations were evaluated after different times by
plate counts in nutrient agar. All the cultures presented growth at 30ºC with the generation time varying from 28.8 to 36.0
minutes. Three cultures also presented growth at 10ºC with generation times between 10.16 and 28.38 h. Considering the results,
it was concluded that meat kept at abusive temperatures would be subject to development of this microorganism.
Tipo: Journal article
Palavras-chave: Bacillus cereus; Meat; Growth.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400022
Molecular analysis of the iap gene of Listeria monocytogenes isolated from cheeses in Rio Grande do
Sul, Brazil
Provedor de dados: 58
Autores: Mello,Jozi Fagundes de; Einsfeldt,Karen; Frazzon,Ana Paula Guedes; Costa,Marisa da; Frazzon,Jeverson.
The polymorphic region sequences in the iap gene were analyzed in 25 strains of Listeria monocytogenes isolated from cheeses in
the state of Rio Grande do Sul, and compared with reference strains. This investigation distinguished two clusters of L.
monocytogenes: I (20 strains) and II (5 strains).
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Iap gene; Cheese; TN amino acids.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100033
Extracellular polysaccharide production by a strain of Pleurotus djamor isolated in the south of
Brazil and antitumor activity on Sarcoma 180
Provedor de dados: 58
Autores: Borges,Gisele Martini; De Barba,Fabiana Figueredo Molin; Schiebelbein,Ana Paula; Pereira,Bruna Parmezzani;
Chaves,Mariane Bonatti; Silveira,Marcia Luciane Lange; Pinho,Mauro Souza Leite; Furlan,Sandra Aparecida; Wisbeck,Elisabeth.
Polysaccharides with medicinal properties can be obtained from fruiting bodies, mycelium and culture broth of several fungus
species. This work was carried out in batch culture using a stirred tank reactor with two different initial glucose concentrations
(40-50 g/L) and pH values (3.0-4.0) to enhance extracellular polysaccharides production by Pleurotus djamor UNIVILLE 001 and
evaluate antitumor effect of intraperitonial administration of Pleurotus djamor extract on sarcoma 180 animal model. According to
factorial design, the low pH value (pH 3.0) led to a gain of 1.6 g/L on the extracellular polysaccharide concentration, while
glucose concentration in the tested range had no significant effect on the concentration of polysaccharide. With 40 g/L initial...
Tipo: Journal article
Palavras-chave: Pleurotus djamor; Extracellular polysaccharides production; Antitumor activity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400006
Search for endophytic diazotrophs in barley seeds
Provedor de dados: 58
Autores: S. Zawoznik,Myriam; C. Vázquez,Susana; Díaz Herrera,Silvana M.; D. Groppa,María.
Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features
were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were
investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro
and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.
Tipo: Journal article
Palavras-chave: Azospirillum; Bacillus; Pseudomonas; PGPR; RAPD.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200033
Engyodontium album fungaemia: the first reported case
Provedor de dados: 58
Autores: Macêdo,Danielle Patrícia Cerqueira; Neves,Rejane Pereira; Souza-Motta,Cristina Maria de; Magalhães,Oliane M.
Correia.
Opportunistic mycoses have been increasingly observed among immunocompromised patients. We describe a case in which
Engyodontium album was isolated and cultured from the blood of a patient with the Acquired Immunodeficiency Syndrome. E.
album grew at 37ºC and showed proteinase activity, both indicators of pathogenicity. This is the first time that this organism has
been reported as agent of fungaemia.
Tipo: Journal article
Palavras-chave: Engyodontium album; Fungaemia; Acquired Immunodeficiency Syndrome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100023
Arbuscular mycorrhizal fungi in papaya plantations of Espírito Santo and Bahia, Brazil
Provedor de dados: 58
Autores: Trindade,Aldo Vilar; Siqueira,José Oswaldo; Stürmer,Sidney Luiz.
The aim of this study was to obtain knowledge on arbuscular mycorrhizal (AM) associations in papaya (Caricapapaya, L.) in field
soils and nursery conditions. Sixty seven soil and root samples were taken in February and May of 1996, from 47 commercial
plantations in the North of Espirito Santo State and the West and South of Bahia State, in Brazil. Samples were used for direct
spore counts, root colonization assessment and for trap culture with Sorghumbicolor (L.) Moench and Crotalariajuncea L.
Additional sampling was done in commercial nurseries to evaluate mycorrhizal colonization. Although papaya cropping systems
are usually under high input of fertilizers and pesticides, papaya roots showed considerable arbuscular mycorrhizal (AM)
colonization, ranging...
Tipo: Journal article
Palavras-chave: Glomeromycota; Agroecosystems; Ecology; Caricapapaya.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300016
ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine
Provedor de dados: 58
Autores: Moreno,Luisa Zanolli; Castilla,Karina Salvagni; Gobbi,Débora Dirani Sena de; Coutinho,Tania Alen; Ferreira,Thais
Sebastiana Porfida; Moreno,Andrea Micke.
Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in
piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the
molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to
characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed
serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the
strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been
related to...
Tipo: Journal article
Palavras-chave: Haemophilus parasuis; Glässer disease; Genotyping; ERIC-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189
Provedor de dados: 58
Autores: Naz,Sehar Afshan; Jabeen,Nusrat; Sohail,Muhammad; Rasool,Sheikh Ajaz.
Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an
opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as,
bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a
clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification,
produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically
relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH.
Furthermore, the pyocin also...
Tipo: Journal article
Palavras-chave: Bacteriocin; Pyocin; P. aeruginosa.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147
Candida albicans morphologies revealed by scanning electron microscopy analysis
Provedor de dados: 58
Autores: Staniszewska,M.; Bondaryk,M.; Swoboda-Kopec,E.; Siennicka,K.; Sygitowicz,G.; Kurzatkowski,W.
Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical
isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/ or CPH1 (strains HLC54 and
Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphologies.
Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and
morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain's and null mutants'
morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed
in...
Tipo: Journal article
Palavras-chave: Candida albicans; Pleomorphism; Ultrastructure; Virulence.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300023
Cross protection by heat and cold shock to lethal temperatures in Clostridium perfringens
Provedor de dados: 58
Autores: García,Santos; Limón,Julio C.; Heredia,Norma L.
A heat shock pre-treatment of Clostridium perfringens provides protection against lethality from subsequent exposure to cold
shock and vice versa. Heat shocked cells were more cold tolerant (one log reduction in 65 min) than control cells (one log
reduction in 34 min). On the other hand, cold-shocked cells were more thermotolerant (D55= 17 min) than the control (D55= 6.5
min). The addition of chloramphenicol (an inhibitor of protein synthesis) in the experiments indicates that synthesis of new
proteins was necessary for cross protection between both treatments.
Tipo: Journal article
Palavras-chave: Clostridium perfringens; Heat shock; Cold shock.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200007
Evaluation of biofilm production by Pseudomonas Aeruginosa isolates recovered from cystic fibrosis
and non-cystic fibrosis patients
Provedor de dados: 58
Autores: Perez,L.R.R.; Costa,M.C.N.; Freitas,A.L.P.; Barth,A.L.
Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that
many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from
sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Biofilm; Cystic fibrosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200011
Toxicological evaluation of vegetable oils and biodiesel in soil during the biodegradation process
Provedor de dados: 58
Autores: Tamada,Ivo S.; Montagnolli,Renato N.; Lopes,Paulo R. M.; Bidoia,Ederio D.
Vegetable oils and their derivatives, like biodiesel, are used extensively throughout the world, thus posing an environmental risk
when disposed. Toxicity testing using test organisms shows how these residues affect ecosystems. Toxicity tests using earthworms
(Eisenia foetida. are widespread because they are a practical resource for analyzing terrestrial organisms. For phytotoxicological
analysis, we used seeds of arugula (Eruca sativa and lettuce (Lactuca sativa. to analyze the germination of seeds in contaminated
soil samples. The toxicological experiment was conducted with four different periods of biodegradation in soil: zero days, 60
days, 120 days and 180 days. The studied contaminants were soybean oil (new and used) and biodiesel (B100). An...
Tipo: Journal article
Palavras-chave: Bioremediation; Toxicity; Soybean oil; Soil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400042
Yield of four Agaricus bisporus strains in three compost formulations and chemical composition
analyses of the mushrooms
Provedor de dados: 58
Autores: Andrade,Meire Cristina Nogueira de; Zied,Diego Cunha; Minhoni,Marli Teixeira de Almeida; Kopytowski Filho,João.
Three compost formulations, consisting of two varieties of Cynodom dactylon (L.) Pers. (Coast-cross and Tyfton) and oat (Avena
sativa) straw were tested for the cultivation of A. bisporus strains ABI-01/01, ABI-04/02, ABI-05/03, and ABI-06/04. A
completely randomized experimental design in a factorial scheme was adopted, with 12 treatments (4 A. bisporus strains × 3 types
of compost) and 8 replicates. Each experimental unit corresponded to one box containing 12 12.5 kg fresh wet compost. The data
were submitted to analysis of variance and the means were compared by Tukey test. According to the results, productivity of
mushrooms was influenced by strain and/or compost type. It was also verified that crude protein, ash, and crude fiber contents in
the...
Tipo: Journal article
Palavras-chave: Agaricus bisporus; Strains; Straw; Nutritional composition; Productivity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300034
Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk
Provedor de dados: 58
Autores: Corrêa,Ana Paula F.; Daroit,Daniel J.; Velho,Renata V.; Brandelli,Adriano.
The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a
Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic
enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High levels of
α-glucosidase were produced in skim milk broth. Hydrolytic enzymes detected in skim milk broth are of particular
concern, indicating that these enzymes could be produced by Pseudomonas sp. during the cold storage of raw milk, contributing to
the spoilage problem in milk and dairy products.
Tipo: Journal article
Palavras-chave: Pseudomonas; Hydrolases; Protease; Lipase; Glycosidase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400034
Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains
Provedor de dados: 58
Autores: Šuranská,Hana; Vránová,Dana; Omelková,Jiřina.
Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them
for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts.
The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South
Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing
were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and
relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and
grouped...
Tipo: Journal article
Palavras-chave: Saccharomyces genus; Oenological properties; ITS-PCR-RFLP; Interdelta PCR typing; Species-specific
primers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100181
Properties of catechol 1,2-dioxygenase in the cell free extract and immobilized extract of
Mycobacterium fortuitum
Provedor de dados: 58
Autores: Silva,A.S.; Jacques,R.J.S.; Andreazza,R.; Bento,F.M.; Roesch,L.F.W.; Camargo,F.A.O.
Polycyclic aromatic hydrocarbons (PAH) are carcinogenic compounds which contaminate water and soil, and the enzymes can be
used for bioremediation of these environments. This study aimed to evaluate some environmental conditions that affect the
production and activity of the catechol 1,2-dioxygenase (C12O) by Mycobacterium fortuitum in the cell free and immobilized
extract in sodium alginate. The bacterium was grown in mineral medium and LB broth containing 250 mg L-1 of anthracene
(PAH). The optimum conditions of pH (4.0-9.0), temperature (5-70 ºC), reaction time (10-90 min) and the effect of ions in the
enzyme activity were determined. The Mycobacterium cultivated in LB shown higher growth and the C12O activity was two-fold
higher to that in the mineral...
Tipo: Journal article
Palavras-chave: Anthracene; Enzyme activity; Enzyme immobilization; Biodegradation; Waste treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100043
Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain
Aspergillus niger B03
Provedor de dados: 58
Autores: Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova.
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The
enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration.
Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values
for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate
specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase
displayed maximum activity in the case...
Tipo: Journal article
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008
Production of ß-Glucosidase by Penicillium purpurogenum
Provedor de dados: 58
Autores: Dhake,A.B.; Patil,M.B.
The fungus Penicillium purpurogenum was found to produce intracellular ß-glucosidase. Maximum activity of ß-glucosidase was
observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of ß-glucosidase were
optimized. Maximum enzyme content was observed after 96 hours of cultivation at 30º;C. Addition of amino acids histidine and
cysteine induced ß-glucosidase synthesis to certain extent. The optimum temperature and pH for ß-glucosidase activity was 50º;C
and 5.5 respectively. ß-glucosidase of P.purpurogenum shows stability at pH 2 thus it could be an ideal enzyme for debittering in
fruit juice and wine industries.
Tipo: Journal article
Palavras-chave: SS-glucosidase; Penicillium.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200013
Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum
gloeosporioide
Provedor de dados: 58
Autores: Yong,H.Y.; Bakar,F.D.A.; Illias,R.M.; Mahadi,N.M.; Murad,A.M.A.
The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and
plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of
MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2
open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function,
a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion.
Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When
grown under...
Tipo: Journal article
Palavras-chave: Colletotrichum gloeosporioides; MAP kinase; Pathogenicity; Appressorium.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400031
Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from
diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb
degradation
Provedor de dados: 58
Autores: Ramya,Shanivarsanthe Leelesh; Venkatesan,Thiruvengadam; Srinivasa Murthy,Kottilingam; Jalali,Sushil Kumar;
Verghese,Abraham.
Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is
resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic
degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify
esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in
India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S
rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates,...
Tipo: Journal article
Palavras-chave: Plutella xylostella; Bacteria; 16S rRNA; Phylogeny; Carboxylesterase; Indoxacarb.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200327
Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
Provedor de dados: 58
Autores: Lüdtke,Charli Beatriz; Coutinho,Mariana Loner; Jouglard,Sandra Denise Dorneles; Moreira,Cecília Nunes;
Fernandes,Cláudia Hartleben Pinho; Brod,Claudiomar Soares; Haake,David A.; Ko,Albert Icksang; Dellagostin,Odir Antonio;
Aleixo,José Antonio Guimarães.
Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of
pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant
LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb
produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and
immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and
from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted
with non-pathogenic Leptospira. The MAbs...
Tipo: Journal article
Palavras-chave: Leptospirosis; Monoclonal antibodies; LipL32.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500001
Bovine herpesvirus 5 detection by virus isolation in cell culture and multiplex-PCR in central nervous
system from cattle with neurological disease in Brazilian herds
Provedor de dados: 58
Autores: Claus,Marlise Pompeo; Alfieri,Alice Fernandes; Médici,Kerlei Cristina; Lunardi,Michele; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 (BoHV-5) is an important cause of meningoencephalitis in young and adult cattle. The multiple etiology of
neurological disturbances in cattle makes the quick and conclusive diagnosis of BoHV-5 infection important for animal and public
health, mainly because of herbivore rabies that is endemic in Brazilian cattle herds. The objective of this retrospective study was
to use a multiplex-polymerase chain reaction (multiplex-PCR) for BoHV-5 and BoHV-1 glycoprotein C gene detection from
stored central nervous system (CNS) tissue fragments of cattle with neurological clinical signs. Forty-seven frozen CNS samples
of young and adult cattle from 31 herds in three Brazilian geographical regions (South, Southeast, and Center-west) were
evaluated....
Tipo: Journal article
Palavras-chave: Cattle; Meningoencephalitis; Bovine herpesvirus 5; Multiplex-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300019
Evaluation of the biological nitrogen fixation contribution in sugarcane plants originated from seeds
and inoculated with nitrogen-fixing endophytes
Provedor de dados: 58
Autores: Canuto,Erineudo de Lima; Oliveira,André Luis Martinez de; Reis,Verônica Massena; Baldani,José Ivo.
The inoculation technique with endophytic diazotrophic bacteria in sugarcane has been shown as an alternative practice to plant
growth promotion. The aim of this work was to evaluate the biological nitrogen fixation (BNF) contribution by different strains of
Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus in sugarcane plant inoculated from seeds. The seeds were
planted in pots filled with non-sterile soil, inoculated with the bacterial strains and grown 10 months outside of the greenhouse.
The BNF contribution of the inoculated bacteria varied depending on the plant species used as a control. The highest BNF
contribution as well as the highest populations of reisolated bacteria was observed with inoculation of H. seropedicae strains.
The...
Tipo: Journal article
Palavras-chave: Endophytic diazotrophic bacteria; Sugarcane; Biological nitrogen fixation; Herbaspirillum seropedicae;
Gluconacetobacter diazotrophicus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500021
Distribution of virulence genes sefC, pefA and spvC in Salmonella Enteritidis phage type 4 strains
isolated in Brazil
Provedor de dados: 58
Autores: Castilla,Karina Salvagni; Ferreira,Claudete Serrano Astolfi; Moreno,Andrea Micke; Nunes,Iolanda Aparecida;
Ferreira,Antônio José Piantino.
The distribution of virulence genes, sefC, pefA and spvC, was investigated in 110 Salmonella Enteritidis phage type 4 strains by
polymerase chain reaction. Their influence in the caecal colonization and invasion of liver and spleen of one-day-old chickens was
studied. Eight isolates were negative for the spvC gene, three for the pefA gene and one, for the sefC gene. These results allowed
grouping the strains into four genotypes. Presence of these genes did not influence bacteria invasion in the liver and spleen of the
chickens ten days after infection, although the presence of more than one fimbrial gene can be related to caecal colonization.
Tipo: Journal article
Palavras-chave: Salmonella; Virulence genes; Colonization; Invasion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200007
Mutations in the quinolone resistance-determining regions of gyrA and parC in Enterobacteriaceae
isolates from Brazil
Provedor de dados: 58
Autores: Minarini,Luciene A. R.; Darini,Ana Lucia C.
Mutations in the quinolone resistance-determining regions (QRDR) in chromosomal gyrA and parC genes and fluoroquinolone
susceptibility profiles were investigated in quinolone-resistant Enterobacteriaceae isolated from community and hospitalized
patientsin the Brazilian Southeast region. A total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to
2005 were investigated for mutations in the topoisomerases genes gyrA and parC by amplifying and sequencing the QRDR
regions. Susceptibility to fluoroquinolones was tested by the agar dilution method. Amongst the 112 enterobacterial isolates, 81
(72.3%) were resistant to ciprofloxacin and 5 (4.5%) showed reduced susceptibility. Twenty-six (23.2%) were susceptible to
ciprofloxacin....
Tipo: Journal article
Palavras-chave: Quinolone resistance-determining region; Mutations; GyrA; ParC; Enterobacteriaceae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400010
Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites
by Polyporus tricholoma Mont.
Provedor de dados: 58
Autores: Vieira,Gladys Rosane Thomé; Liebl,Mariane; Tavares,Lorena Benathar Ballod; Paulert,Roberta; Smânia Júnior,Artur.
Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial
compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that
could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on
biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The
initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth
rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence...
Tipo: Journal article
Palavras-chave: Polyporus tricholoma; Specific growth rate; Antibacterial activity; Isodrimenediol.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300029
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp.
AB68
Provedor de dados: 58
Autores: Aygan,Ashabil; Arikan,Burhan; Korkmaz,Hatice; Dinçer,Sadik; Çolak,Ömer.
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme
synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which
was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at
50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over
90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of
remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The
activity of the enzyme was...
Tipo: Journal article
Palavras-chave: Bacillus sp. α -amylase alkaliphilic thermostable enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027
The use of acetonitrile as the sole nitrogen and carbon source by Geotrichum sp. JR1
Provedor de dados: 58
Autores: Rezende,Rachel Passos; Dias,João Carlos Teixeira; Monteiro,Andrea Souza; Carraza,Fernando; Linardi,Valter Roberto.
A yeast strain identified as Geotrichum sp. JR1 was able to use acetonitrile as the sole carbon and nitrogen source. The strain grew
in 0.5 to 2M acetonitrile. Ammonia generation as enzymatic product during the strain growth indicates the presence of an
acetonitrile degrading enzyme. Acetic acid and acetamide were detected during assays with the resting cells cultivated in
acetonitrile, indicating the presence of nitrile and amide degrading enzymes. This paper is the first to describe the use of
acetonitrile as the sole carbon and nitrogen source by a yeast.
Tipo: Journal article
Palavras-chave: Geotrichum sp.; Acetonitrile; Degrading enzymes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100019
Leptospires detection in kidney, liver and uterus of cows slaughtered in Paraná State, Brazil
Provedor de dados: 58
Autores: Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson;
Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda.
The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary
status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field
microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH
modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and
indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14),
from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct
examination detected...
Tipo: Journal article
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008
Antimicrobial properties of sclerotiorin, isocHromophilone VI and pencolide, metabolites from a
Brazilian cerrado isolate of Penicillium sclerotiorum van Beyma
Provedor de dados: 58
Autores: Lucas,Esther M.F.; Castro,Mateus C. Monteiro de; Takahashi,Jacqueline A.
As a part of a research program that aims to identify antibacterial and antifungal substances from fungus specimen of Brazilian's
cerrado soil samples, Penicillium sclerotiorum was identified as a source of secondary metabolites possessing antibiotic activities.
This microorganism was cultured in a liquid medium rich in glucose for fifteen days. The resulting ethyl acetate extract was
chemically fractionated leading to the isolation of three metabolites pencolide, sclerotiorin and isochromophilone VI. The
antimicrobial disc assay activity of these substances towards Candida albicans, Streptomyces pyogenes, Staphylococcus aureus,
Salmonella typhimurium and Escherichia coli was performed. Minimum inhibitory concentration (MIC) of the compounds was...
Tipo: Journal article
Palavras-chave: Sclerotiorin; Isocromophilone VI; Pencolide; Antibiotics; Penicillium sclerotiorum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400036
Echovirus 6 associated to aseptic meningitis outbreak, in São Joaquim da Barra, São Paulo, Brazil
Provedor de dados: 58
Autores: Luchs,Adriana; Russo,Denise Hage; Cilli,Audrey; Costa,Fernanda Faria; Morillo,Simone Guadagnucci;
Machado,Bráulio Caetano; Pellini,Alessandra Cristina Guedes; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do
Carmo Sampaio Tavares.
An aseptic meningitis outbreak occurred during a period from February to May 2004 in São Joaquim da Barra, a town in the
northern region of São Paulo State. A total of 40 cases were reported to the Epidemiological Surveillance Center of São Paulo
State. Cerebrospinal fluid samples obtained from 23 patients were sent to the Adolfo Lutz Institute for isolation of the virus. These
samples were inoculated into RD, HEp2 and Vero cell lineages and those presenting a cytopathogenic effect were selected for
analysis by indirect immunofluorescence assay (IFA), neutralization testing (Nt) and reverse transcriptase-polymerase chain
reaction (RT-PCR). Cytopathogenic effects were observed in 52.2% (12/23) of these samples. All isolated viruses were identified
as human...
Tipo: Journal article
Palavras-chave: Enterovirus surveillance; Echovirus 6; Aseptic meningitis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100007
Growth, fatty acid profile in major lipid classes and lipid fluidity of Aurantiochytrium mangrovei
Sk-02 as a function of growth temperature
Provedor de dados: 58
Autores: Chodchoey,Kanokwan; Verduyn,Cornelis.
Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15
g/L) at temperatures ranging from 12 to 35°C. The fastest growth (µmax= 0.15 h-1) and highest fatty acid content of 415 mg/g-dry
cell weight were found in the cells grown at 30°C. However, the cells grown at 12°C showed the highest percentage of
polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic
acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA
(C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly
affected by the...
Tipo: Journal article
Palavras-chave: Anisotropy measurement; Aurantiochytrium sp.; Docosahexaenoic acid; Lipid class composition; Membrane
fluidity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100020
Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a
microprocessor controlled bioreactor
Provedor de dados: 58
Autores: Mukhtar,Hamid; Ikram-ul-Haq,.
We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled
laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two
industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of
alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in
the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum
production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The
optimum...
Tipo: Journal article
Palavras-chave: Simultaneous; Sporulation; Kinetics; Fermentation; Agitation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033
Phage amplification assay as rapid method for Salmonella detection
Provedor de dados: 58
Autores: Siqueira,Regina Silva de; Dodd,Christine E.R.; Rees,Catherine E.D.
The application of rapid methods is crucial for the HACCP program implantation in food industry. In this context, Phage
Amplification Assay is a good candidate because is based on the interactions of phage and their host bacteria. This method using
phage P22 was applied with to detect Salmonella cells in chicken breast. Samples of 25 g of chicken breast were diluted and the
appropriate dilutions were used in phage amplification assay for Salmonella detection. After 3-4 h of incubation, it was observed a
phage titre of approximately 10(4) pfu mL-1, indicating that there were Salmonella cells which were naturally present in the meat.
The presence of Salmonella cells were verified by using direct plating on XLD agar and by conventional enrichment procedure....
Tipo: Journal article
Palavras-chave: Salmonella; Rapid method; Bacteriophage; P22.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500040
Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of
southeast Brazil
Provedor de dados: 58
Autores: Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da.
Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of
shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children
aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains
being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to
investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in
the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated...
Tipo: Journal article
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034
Distribution of aflatoxins in corn fractions visually segregated for defects
Provedor de dados: 58
Autores: Piedade,Fabiana Segatti; Fonseca,Homero; Gloria,Eduardo Micotti da; Calori-Domingues,Maria Antonia; Piedade,Sônia
Maria Stefano; Barbin,Décio.
The aflatoxin distribution in corn fractions obtained after visual segregation for defects in 30 samples, known to be contaminated,
was studied. Each sample was passed through a 5.0 mm round holes sieve, graded for defects and then segregated in sound kernels
(regular kernels) and non-sound kernels (injured, germinated, fermented, moldy, heated, insect damaged, immature, broken,
hollow, fermented up to ¼, discolored, extraneous materials, and injured by other causes), as defined by the Brazilian Official
Grading rules for corn. The non-sound kernels showed the highest contamination levels in all samples. The contamination levels
of non-sound kernels (20% of total weight) ranged from 23 to 1,365 µg/kg of aflatoxins (B1, B2, G1 and G2) and were higher
than...
Tipo: Journal article
Palavras-chave: Aflatoxins; Corn; Contamination distribution; Visual segregation; Fraction.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300013
Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil
Provedor de dados: 58
Autores: Sartoratto,Adilson; Machado,Ana Lúcia M.; Delarmelina,Camila; Figueira,Glyn Mara; Duarte,Marta Cristina T.;
Rehder,Vera Lúcia G.
Essential oils from aerial parts of Mentha piperita, M. spicata, Thymus vulgaris, Origanum vulgare, O. applii, Aloysia triphylla,
Ocimum gratissimum, O. basilicum were obtained by steam destillation using a Clevenger-type system. These oils were screened
for antibacterial and anti-Candida albicans activity using bioautographic method. Subsequently, minimal inhibitory concentration
from oils was determined by microdilution method. Most essential oil studied were effective against Enterococcus faecium and
Salmonella cholerasuis. Aloysia triphylla and O. basilicum presented moderate inhibition against Staphylococcus aureus while
only A. tryphila and M. piperita were able to control the yeast Candida albicans. The oils were analyzed by GC and GC-MS
techniques...
Tipo: Journal article
Palavras-chave: Essential oil; Medicinal plants; Antimicrobial activity; Minimal inhibitory concentration.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300001
Optimization of exopolysaccharides production from a novel strain of Ganoderma lucidum CAU5501
in submerged culture
Provedor de dados: 58
Autores: Yuan,Baojing; Chi,Xiaoyan; Zhang,Rijun.
This study aimed at optimizing the medium of a new Ganoderma lucidum strain CAU5501 to enhance the yield of
exopolysaccharides (EPS) and mycelial growth. Firstly, the suitable level of glucose, magnesium, phosphate and C/N ratio was
determined by single factor experiment. Subsequently, the optimum concentrations of these medium components were
investigated using the orthogonal matrix method. The results indicated that the higher levels of EPS were correlated with the level
of cell growth when glucose concentration was studied (data no show). The optimum medium for EPS yield was found to be 70
g/l glucose, 5 C/N ratio, 2.5 g/l KH2PO4, 0.75 g/l MgSO4·7H2O, and for mycelial growth was 50 g/l glucose, 5 C/N ratio, 1.5 g/l
KH2PO4, 0.5 g/l MgSO4·7H2O. When...
Tipo: Journal article
Palavras-chave: Optimization; Exopolysaccharides; Ganoderma lucidum; Orthogonal matrix method; Submerged culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200009
Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with
freshwater ornamental fish culture systems: a possible threat to public health
Provedor de dados: 58
Autores: Sreedharan,Krishnan; Philip,Rosamma; Singh,Isaac Sarojani Bright.
Aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including
fishes and humans. In the present study, water samples from two ornamental fish culture systems were analyzed for the presence
of Aeromonas. Nutrient agar was used for Aeromonas isolation, and colonies (60 No) were identified through biochemical
characterization. Seven clusters could be generated based on phenotypic characters, analyzed by the programme NTSYSpc,
Version 2.02i, and identified as: Aeromonas caviae (33.3%), A. jandaei (38.3%) and A. veronii biovar sobria (28.3%). The strains
isolated produced highly active hydrolytic enzymes, haemolytic activity and slime formation in varying proportions. The isolates
were also...
Tipo: Journal article
Palavras-chave: Aeromonas; Antibiotic susceptibility; Ornamental fish culture systems; Virulence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200040
Evaluating most probable number method to count and isolate viable methylotrophs
Provedor de dados: 58
Autores: Kashyap,S.
Nine different receptacles were tested with the MPN method to determine which receptacle was most reliable and economical for
MPN counts. Results showed that 96 well PCR plate were the best vessels for this type of analysis and facilitated the isolation of
viable Methylotrophs.
Tipo: Journal article
Palavras-chave: Methylotrophs; MPN; Receptacle.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100006
Pseudomonas aeruginosa KUCd1, a possible candidate for cadmium bioremediation
Provedor de dados: 58
Autores: Sinha,Sangram; Mukherjee,Samir Kumar.
A cadmium (8 mM) resistant Pseudomonas aeruginosa strain KUCd1 exhibiting high Cd accumulation under in vitro aerobic
condition has been reported. The isolate showed a significant ability to remove more than 75% and 89% of the soluble cadmium
during the active growth phase from the growth medium and from Cd-amended industrial wastewater under growth supportive
condition. Transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDXS) suggest the presence of
Cd in the cells from mid-stationary phase. The cell fractionation study revealed membrane and periplasm to be the major
accumulating site in this strain. The chemical nature of the accumulated Cd was studied by X-ray powder diffraction analysis.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Cadmium; Bioremediation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300030
Use of Bioscreen C for growth of Mucor hiemalis in different carbon and nitrogen sources
Provedor de dados: 58
Autores: Tauk-Tornisielo,Sâmia Maria; Vieira,Joelma Maurício; Govone,José Silvio.
The automated Bioscreen C system was used for growth of four Mucor hiemalis strains isolated from the soil in the Juréia-Itatins
Ecology Station (JIES), São Paulo state, in liquid culture media containing different carbon (2%) and nitrogen (1%) sources, pH
5.0, at 25ºC, and agitated at 150 rpm for 5 days. The medium containing only one nitrogen source had been added with 2%
glucose. The readings were taken at lambda = 540 nm, at 2-hour intervals, up to five days. The results were compared using the
Friedman Test (alpha = 5%). The best growth was obtained for strains M1, M2 and M3, reaching the log phase in 60 hours. The
best carbon sources varied according to the strain, and yeast extract proved to be the best nitrogen source. Sodium acetate
inhibited the...
Tipo: Journal article
Palavras-chave: Mucor hiemalis; Zygomycetes; Automated systems; Carbon and nitrogen sources.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100024
Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and
Salmonella enterica subsp enterica serovar Gallinarum
Provedor de dados: 58
Autores: Ribeiro,Simone Alves Mendes; Paiva,Jaqueline Boldrin de; Zotesso,Fábio; Lemos,Manoel Victor Franco; Berchieri
Júnior,Ângelo.
S. Pullorum (SP) and S. Gallinarum (SG) are very similar. They are the agents of pullorum disease and fowl typhoid, respectively,
and the two diseases are responsible for economic losses in poultry production. Although SP and SG are difficult to be
differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve
this aim. While SP is able to decarboxylate this amino acid, SG is not. However, the isolation of strains showing atypical
biochemical behavior has made this differentiation difficult. One of the genes associated with the metabolization of the amino acid
ornithine is called speC, and is found in both serovars. The analysis of 21 SP and 15 SG strains by means of PCR did not enable...
Tipo: Journal article
Palavras-chave: Salmonella Pullorum; Salmonella Gallinarum; Differentiation; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100032
Endophytic fungi from Myrcia guianensis at the Brazilian Amazon: distribution and bioactivity
Provedor de dados: 58
Autores: Banhos,Elissandro Fonseca dos; Souza,Antonia Queiroz Lima de; Andrade,Juliano Camurça de; Souza,Afonso Duarte
Leão de; Koolen,Hector Henrique Ferreira; Albuquerque,Patrícia Melchionna.
Beneficial interactions between plants and microorganisms have been investigated under different ecological, physiological,
biochemical, and genetic aspects. However, the systematic exploration of biomolecules with potential for biotechnological
products from this interaction still is relatively scarce. Therefore, this study aimed the evaluation of the diversity and antimicrobial
activity of the endophytic fungi obtained from roots, stems and leafs of Myrcia guianensis (Myrtaceae) from the Brazilian
Amazon. 156 endophytic fungi were isolated and above 80% were identified by morphological examination as belonging to the
genera Pestalotiopsis, Phomopsis, Aspergillus, Xylaria, Nectria, Penicillium and Fusarium. Fermented broth of those fungi were
assayed for...
Tipo: Journal article
Palavras-chave: Secondary metabolites; Fungus/plant interaction; Antibiosis; Amazonian endophytic fungi.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100021
Effectiveness of Metarhizium anisopliae against immature stages of Anastrepha fraterculus fruitfly
(Diptera : Tephritidae)
Provedor de dados: 58
Autores: Destéfano,Ricardo Henry Rodrigues; Bechara,Ivanira José; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico.
The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales) strain E9, isolated
from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae), against larvae, prepupae and pupae stage and emergent
adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on
autoclaved (AS) and non-autoclaved (NAS) soil from typical citrus orchards in the State of São Paulo, Southeastern region of
Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult
emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10) for AS and 2.52 x 10(10) for NAS. The lethal
concentration...
Tipo: Journal article
Palavras-chave: Microbial control; Metarhizium anisopliae; Anastrepha fraterculus; Fruitfly; Bioassay.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100018
Modification of c and n sources for enhanced production of cyclosporin 'a' by Aspergillus Terreus
Provedor de dados: 58
Autores: Tanseer,Sundas; Anjum,Tehmina.
Most of the studies regarding cyclosporin 'A' production through fungi concentrate around Tolypocladium inflatum. This is
mainly due to lower reported production of this drug in other fungi. The present study was therefore conducted to explore
indigenous isolates of Aspergillus terreus for synthesis of this drug and defining a production medium for obtaining high yield of
cyclosporin 'A'. For this purpose carbon and nitrogen sources were optimized for the selected best strain of A. terreus. Overall
results depicted that the best cyclosporin 'A' yield from selected Aspergillus terreus (FCBP58) could be obtained by using
production medium containing glucose 10% as carbon source and peptone 0.5% as nitrogen source. This modification in
production medium...
Tipo: Journal article
Palavras-chave: Aspergillus terreus; Cyclosporin 'A'; Carbon; Nitrogen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400019
Mode of action and in vitro susceptibility of mastitis pathogens to macedocin ST91KM and
preparation of a teat seal containing the bacteriocin
Provedor de dados: 58
Autores: Pieterse,Renee; Todorov,Svetoslav D.; Dicks,Leon M.T.
Mastitis is considered to be the most economically costly disease affecting the dairy industry. Regular dosage of animals with
antibiotics, including use of prophylactic concentrations, may select for resistant strains. The purpose of this study was to
determine the mode of action of a new bacteriocin (macedocin ST91KM), to evaluate the antimicrobial resistance of mastitis
pathogens to antibiotics commonly used in treatment remedies, and to introduce the possible use of an alternative antimicrobial
agent. The bacteriocin macedocin ST91KM, produced by Streptococcus gallolyticus subsp. macedonicus ST91KM, is bactericidal
to Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Staphylococcus aureus associated with
mastitis...
Tipo: Journal article
Palavras-chave: Macedocin ST91KM; Activity against mastitis pathogens; Teat seal preparation; Milk.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100020
Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in
mules
Provedor de dados: 58
Autores: Silva,Karla Patrícia Chaves da; Takaki,Galba Maria de Campos; Silva,Leonildo Bento Galiza da; Saukas,Tomoe Noda;
Santos,André Souza; Mota,Rinaldo Aparecido.
To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders.
These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no
clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation
with trichlo-roa-cetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by
Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of
PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control
group showed no...
Tipo: Journal article
Palavras-chave: Mallein test; Equus asinus; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100027
Correlations between symptoms and DAS - Elisa values in two sources of resistance against tomato
spotted wilt virus
Provedor de dados: 58
Autores: Resende,Luciane V.; Maluf,Wilson R.; Figueira,Antônia dos R.; Resende,Juliano T.V.
The commercial tomato, Lycopersicon esculentum, cultivars Ângela Gigante I-5100 and Santa Clara (tospovirus susceptible), the
Stevens cultivar, lines and experimental hybrids (tospovirus resistant), were used: a) to evaluate two sources of resistance to
tospoviruses in tomato (one from L. esculentum/Rey de Los Tempranos, and another one from L. peruvianum/Stevens); and b) to
verify statistically significant correlation between virus concentration determined through DAS-ELISA and symptomatology. In
the Ângela Gigante I-5100 and the Santa Clara cultivars, the correlation coefficient was highly significant and the symptom
average level was high, showing that in susceptible cultivars the symptom evolution is related to the virus multiplication in the
plant. As...
Tipo: Journal article
Palavras-chave: Tospovirus; Breed lines; Viruses infection.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200014
Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in
Cochin, India
Provedor de dados: 58
Autores: Parvathi,Ammini; Krishna,Kiran; Jose,Jiya; Joseph,Neetha; Nair,Santha.
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study,
Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical
methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus
pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus
isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for
known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and
lipase...
Tipo: Journal article
Palavras-chave: Bacillus pumilus; FAME; 16S rDNA; CesB; AP-PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200012
Bioconversion of ginsenoside Rb1 into compound K by Leuconostoc citreum LH1 isolated from
kimchi
Provedor de dados: 58
Autores: Quan,Lin-Hu; Piao,Jin-Ying; Min,Jin-Woo; Yang,Dong-Uk; Lee,Hee Nyeong; Yang,Deok Chun.
About 40 different types of ginsenoside (ginseng saponin), a major pharmacological component of ginseng, have been identified
along with their physiological activities. Among these, compound K has been reported to prevent the development of and the
metastasis of cancer by blocking the formation of tumors and suppressing the invasion of cancerous cells. In this study,
ginsenoside Rb1 was converted into compound K via interaction with the enzyme secreted by β-glucosidase active
bacteria, Leuconostoc citreum LH1, extracted from kimchi. The optimum time for the conversion of Rb1 to compound K was
about 72 hrs at a constant pH of 6.0 and an optimum temperature of about 30ºC. Under optimal conditions, ginsenoside Rb1 was
decomposed and converted into...
Tipo: Journal article
Palavras-chave: Bioconversion; Compound K; Ginsenoside Rb1; Leuconostoc citreum LH1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300049
High incidence of oncogenic HPV genotypes found in women from Southern Brazil
Provedor de dados: 58
Autores: Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.;
Collares,Tiago; Seixas,Fabiana K.
Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to
determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations.
Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a
cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used
for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV
infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were
considered significant at p <...
Tipo: Journal article
Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043
Effect of culture medium on biocalcification by Pseudomonas Putida, Lysinibacillus Sphaericus and
Bacillus Subtilis
Provedor de dados: 58
Autores: Shirakawa,Márcia Aiko; Cincotto,Maria Alba; Atencio,Daniel; Gaylarde,Christine C.; John,Vanderley M.
The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus,
Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade
em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture
media were studied: modified B4 containing calcium acetate and 295 with calcium chloride. Calcium consumption in the two
media after incubation with and without the bacterial inoculum was determined by atomic absorption analysis. Modified B4 gave
the best results and in this medium Pseudomonas putida INQCS 113 produced the highest calcium carbonate precipitation,
followed by...
Tipo: Journal article
Palavras-chave: Pseudomonas putida; Lysinibacillus sphaericus; Bacillus subtilis; Calcium carbonate; Biocalcification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200014
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices
Provedor de dados: 58
Autores: Dellagnezze,Bruna Martins; Vasconcellos,Suzan Pantaroto de; Melo,Itamar Soares de; Santos Neto,Eugênio Vaz dos;
Oliveira,Valéria Maia de.
ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge
throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude
oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from
environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different
matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments
using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization
was carried out...
Tipo: Journal article
Palavras-chave: Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712
Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens
of dyspeptic patients
Provedor de dados: 58
Autores: Ramis,Ivy Bastos; Moraes,Ernani Pinho de; Fernandes,Márcia Silveira; Mendoza-Sassi,Raul; Rodrigues,Obirajara;
Juliano,Carlos Renan Varela; Scaini,Carlos James; Silva,Pedro Eduardo Almeida da.
Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of
gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for
detecting H. pylori, however they all have limitations, thus it is recommend a combination of at least two methods. The aim of this
study was to evaluate diagnostic methods, such as in-house urease test, culture and Polymerase Chain Reaction (PCR), for the
detection of the H. pylori in gastric biopsy specimens of 144 dyspeptic patients, using as gold standard the association between
histology and rapid urease test. According to the gold standard used in this study, 48 (33.3%) patients were infected with...
Tipo: Journal article
Palavras-chave: Helicobacter pylori; Histology; Culture; Urease test; Polymerase Chain Reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300008
Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization
Provedor de dados: 58
Autores: Barbosa,Maria Angélica G.; Rehn,Kurt Georg; Menezes,Maria; Mariano,Rosa de Lima R.
The verrucose caused by Cladosporium herbarum reduces production and quality of Passion fruit (Passiflora edulis Sims.), a
largely consumed tropical fruit. This work aimed to investigate the antagonism of Trichoderma species (T. polysporum, T.
koningii, T. viride and T. harzianum) against Cladosporium herbarum, and to study the production of extracellular hydrolytic
enzymes by the pathogen and the antagonists. The results showed considerable antagonistic potential for the biocontrol of C.
herbarum isolates by all Trichoderma species, except T. koningii. The most distinguished effect was observed for T. polysporum.
In relation to the pattern of esterase obtained by electrophoresis in poliacrylamyde gel, the major activity presented by the isolates
was...
Tipo: Journal article
Palavras-chave: Passion fruit; Biological control; Extracellular enzymes; Fungi.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200005
Gordonia polyisoprenivorans from groundwater contaminated with landfill leachate in a subtropical
area: characterization of the isolate and exopolysaccharide production
Provedor de dados: 58
Autores: Fusconi,Roberta; Godinho,Mirna Januária Leal; Hernández,Isara Lourdes Cruz; Bossolan,Nelma Regina Segnini.
A strain of Gordonia sp. (strain Lc), from landfill leachate-contaminated groundwater was characterized by polyphasic taxonomy
and studied for exopolysaccharide (EPS) production. The cells were Gram-positive, catalase-positive, oxidase-negative and
non-motile. The organism grew both aerobically and, in anoxic environment, in the presence of NaNO3. Rods occured singly, in
pairs or in a typical coryneform V-shaped. The organism had morphological, physiological and chemical properties consistent
with its assignment to the genus Gordonia and mycolic and fatty acid pattern that corresponded to those of G. polyisoprenivorans
DSM 44302T. The comparison of the sequence of the first 500 bases of the 16S rDNA of strain Lc gave 100% similarity with the
type strain of...
Tipo: Journal article
Palavras-chave: Gordonia polyisoprenivorans; Exopolysaccharide; Groundwater; Landfill.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200014
Copper influence on polyphosphate metabolism of Cunninghamella elegans
Provedor de dados: 58
Autores: Souza,Patrícia Mendes de; Marinho,Petrusk Homero; Lima,Marcos Antônio Barbosa de; Nascimento,Aline Elesbão do;
Takaki,Galba Maria de Campos.
The aim of this work was to evaluate the physiological aspects of polyphosphate metabolism of Cunninghamella elegans grown in
presence of copper. The growth profile was obtained by means of biomass yields, orthophosphate consumption, polyphosphate
accumulation and phosphatases activities. The results revealed the influence of copper on the growth, observed by biomass yields.
Orthophosphate consumption was faster in cells grown in the presence of copper. The presence of copper in the culture medium
induced polyphosphate accumulation. The polyphosphate level was almost constant in the beginning of control culture growth,
and could be related to the exponential growth phase. On the other hand, the copper treated cultures exhibited a significant
reduction in...
Tipo: Journal article
Palavras-chave: Cunninghamella elegans; Copper; Polyphosphate; Phosphatases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400002
Occurrence of subtilase cytotoxin and relation with other virulence factors in verocytotoxigenic
Escherichia coli isolated from food and cattle in Argentina
Provedor de dados: 58
Autores: Velandia,Claudia V. Granobles; Mariel Sanso,A.; Krüger,Alejandra; Suárez,Lorena V.; Lucchesi,Paula M. A.;
Parma,Alberto E.
We investigated the presence of the gene of subtilase cytotoxin (SubAB), described in certain highly virulent verocytotoxigenic E.
coli strains, in isolates from Argentina and its relation with other virulence factors. The gene subA was present in eae-negative
strains mostly associated with saa, vt2 and ehxA genes.
Tipo: Journal article
Palavras-chave: Subtilase; VTEC; Megaplasmid; Verotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200037
Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces
clavuligerus ATCC 27064
Provedor de dados: 58
Autores: Vasconcelos,Eliton da Silva; Lima,Vanderlei Aparecido de; Goto,Leandro Seiji; Cruz-Hernández,Isara Lourdes;
Hokka,Carlos Osamu.
Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and
cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and
nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement
using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of
bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability
phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are
compared with the...
Tipo: Journal article
Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Mutation; Lipase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400005
Prokaryotic communities of acidic peatlands from the southern Brazilian Atlantic Forest
Provedor de dados: 58
Autores: Etto,R. M.; Cruz,L. M.; Jesus,E. C.; Galvão,C. W.; Galvão,F.; Souza,E. M.; Pedrosa,F. O.; Steffens,M. B. R.
The acidic peatlands of southern Brazil are ecosystems essential for the maintenance of the Atlantic Forest, one of the 25 hot-spots
of biodiversity in the world. In this work, we investigated the composition of prokaryotic communities in four histosols of three
acidic peatland regions by constructing small-subunit (SSU) rRNA gene libraries and sequencing. SSU rRNA gene sequence
analysis showed the prevalence of Acidobacteria (38.8%) and Proteobacteria (27.4%) of the Bacteria domain and Miscellaneous
(58%) and Terrestrial (24%) groups of Crenarchaeota of the Archaea domain. As observed in other ecosystems, archaeal
communities showed lower richness than bacterial communities. We also found a limited number of Euryarchaeota and of known
methanotrophic...
Tipo: Journal article
Palavras-chave: Acidic peatland; Prokaryotic communities; SSU rRNA gene; Microbial ecology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200031
Evaluation of different cryoprotective agents in maintenance of viability of Helicobacter pylori in
stock culture media
Provedor de dados: 58
Autores: Oskouei,Daryoush Davoudi; Bekmen,Neslihan; Ellidokuz,Hülya; Yilmaz,Özlem.
Four different cryoprotective supplemented stock media were evaluated for maintaining better survival and recovery of H. pylori
type strain NCTC 11637 at two different maintenance temperatures of -20°C and -80°C after one month preservation as frozen
stocks. The spread plate colony count method was used to investigate the recovery rate of H. pylori from equally inoculated
bacterial suspensions in differently prepared stock cultures. After the preservation of H. pylori for one month in different
cryoprotectant-supplemented stock media, the recovery rates for -20°C obtained for stock cultures supplemented with dimethyl
sulfoxide (DMSO), polyethylene glycol (PEG), glycerol and glycerol+sucrose, as well as controls with and without human serum
alone were 7.13,...
Tipo: Journal article
Palavras-chave: Helicobacter pylori; Recovery rate; Stock culture media; Cryoprotective agents.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400023
Fumonisins in corn cultivars in the state of São Paulo
Provedor de dados: 58
Autores: Camargos,Simone M.; Soares,Lucia M. Valente; Sawazaki,Eduardo; Bolonhezi,Denizart; Castro,Jairo L.;
Bortolleto,Nelson.
Twenty three samples, belonging to 19 corn cultivars with distinct types of germoplasms, endosperm and length of vegetative
cycle, were analyzed for fumonisins B1 and B2. The cultivars were grown in experimental fields in three locations (Votuporanga,
Ribeirão Preto and Capão Bonito) within the State of São Paulo, Brazil, during the 97/98 crop. All samples were contaminated
with fumonisins with concentrations ranging from 1.63 µg/g to 25.69 µg/g with an average of 5.61 µg/g for FB1 and from 0.38
µg/g to 8.60 µg/g with an average of 1.86 µg/g for FB2. In terms of fumonisins, these high levels put the corn cultivated in São
Paulo among the most contaminated in the world reported to date.
Tipo: Journal article
Palavras-chave: Mycotoxins; Fumonisins; Fusarium; Corn.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300015
Comparison of radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa forel in two
culture media
Provedor de dados: 58
Autores: Miyashira,C.H; Tanigushi,D.G; Gugliotta,A.M; Santos,D.Y.A.C.
In vitro culture of the mutualistic fungus of leaf-cutting ants is troublesome due to its low growth rate, which leads to storage
problems and contaminants accumulation. This paper aims at comparing the radial growth rate of the mutualistic fungus of Atta
sexdens rubropilosa Forel in two different culture media (Pagnocca B and MEA LP). Although total MEA LP radial growth was
greater all along the bioassay, no significant difference was detected between growth efficiencies of the two media. Previous
evidences of low growth rate for this fungus were confirmed. Since these data cannot point greater efficiency of one culture
medium over the other, MEA LP medium is indicated for in vitro studies with this mutualistic fungus due its simpler composition
and...
Tipo: Journal article
Palavras-chave: Leaf-cutting ants; Mutualistic fungus growth; Mycelial growth; Leucoagaricus; Atta sexdens.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200035
Detection of Rickettsia bellii and Rickettsia amblyommii in Amblyomma longirostre (Acari: Ixodidae)
from Bahia state, Northeast Brazil
Provedor de dados: 58
Autores: McIntosh,Douglas; Bezerra,Rodrigo Alves; Luz,Hermes Ribeiro; Faccini,João Luiz Horacio; Gaiotto,Fernanda Amato;
Giné,Gastón Andrés Fernandez; Albuquerque,George Rego.
Studies investigating rickettsial infections in ticks parasitizing wild animals in the Northeast region of Brazil have been confined
to the detection of Rickettsia amblyommii in immature stages of Amblyomma longirostre collected from birds in the state of
Bahia, and in immatures and females of Amblyomma auriculariumcollected from the striped hog-nosed skunk (Conepatus
semistriatus) and armadillos (Euphractus sexcinctus) in the state of Pernambuco. The current study extends the distribution of R.
amblyommii (strain Aranha), which was detected in A. longirostre collected from the thin-spined porcupine Chaetomys
subspinosus and the hairy dwarf porcupine Coendou insidiosus. In addition, we report the first detection of Rickettsia bellii in
adults of A....
Tipo: Journal article
Palavras-chave: Rickettsia amblyommii; Rickettsia bellii; Porcupine; Bahia; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300879
Bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggregation
under salt stress
Provedor de dados: 58
Autores: Qurashi,Aisha Waheed; Sabri,Anjum Nasim.
To compensate for stress imposed by salinity, biofilm formation and exopolysaccharide production are significant strategies of
salt tolerant bacteria to assist metabolism. We hypothesized that two previously isolated salt-tolerant strains Halomonas variabilis
(HT1) and Planococcus rifietoensis (RT4) have an ability to improve plant growth, These strains can form biofilm and accumulate
exopolysacharides at increasing salt stress. These results showed that bacteria might be involved in developing microbial
communities under salt stress and helpful in colonizing of bacterial strains to plant roots and soil particles. Eventually, it can add
to the plant growth and soil structure. We investigated the comparative effect of exopolysacharide and biofilm formation...
Tipo: Journal article
Palavras-chave: Biofilm; Cicer arietinum; Exopolysaccharide; Salinity; Soil aggregates.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300046
Scanning electron microscopy of conidia of Trichoderma stromaticum, a biocontrol agent of witches'
broom disease of cocoa
Provedor de dados: 58
Autores: Melo,Itamar Soares de; Faull,Jane L.
A field emission electron microscope (SEM) was used to study the conidia surface of Trichoderma stromaticum, a biocontrol of
witches broom disease of cocoa. Surface features of conidia were difficult distinguish by light microscope. Conidia appeared to be
verrugose and minutely roughened, but the nature of the roughening was not easy to discern. It was common to observe
sheath-like structures that completely covered groups of conidia, and also details of wide cells that form the pustules.
Tipo: Journal article
Palavras-chave: Biological control; Cocoa; Crinipellis pernisiosa; Morphology of conidia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300011
Optimizing a culture medium for biomass and phenolic compounds production using Ganoderma
lucidum
Provedor de dados: 58
Autores: Zárate-Chaves,Carlos Andrés; Romero-Rodríguez,María Camila; Niño-Arias,Fabián Camilo; Robles-Camargo,Jorge;
Linares-Linares,Melva; Rodríguez-Bocanegra,María Ximena; Gutiérrez-Rojas,Ivonne.
The present work was aimed at optimizing a culture medium for biomass production and phenolic compounds by using
Ganoderma lucidum. The culture was optimized in two stages; a Plackett-Burman design was used in the first one for identifying
key components in the medium and a central composite design was used in the second one for optimizing their concentration.
Both responses (biomass and phenolic compounds) were simultaneously optimized by the latter methodology regarding
desirability, and the optimal concentrations obtained were 50.00 g/L sucrose, 13.29 g/L yeast extract and 2.99 g/L olive oil.
Maximum biomass production identified in these optimal conditions was 9.5 g/L and that for phenolic compounds was 0.0452
g/L, this being 100% better than that...
Tipo: Journal article
Palavras-chave: Medium optimization; Ganoderma lucidum; Plackett-Burman; Central composite design; Phenolic compounds.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100032
Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions
Provedor de dados: 58
Autores: Gou,Yafeng; Li,Jianhua; Zhu,Junbao; Xu,Wanyun; Gao,Jianfeng.
A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke collected from Shihezi (Xinjiang,
China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an Aspergillus niger strain by analysis of
16S rRNA. To improve inulinase production, this fungus was subjected to mutagenesis induced by 60Co γ-irradiation.
A genetically stable mutant (designated E12) was obtained and it showed 2.7-fold higher inulinase activity (128 U/mL) than the
parental strain in the supernatant of a submerged culture. Sequential methodology was used to optimize the inulinase production
of stain E12. A screening trial was first performed using Plackett-Burman design and variables with statistically significant...
Tipo: Journal article
Palavras-chave: Inulin; Ethanol fermentation; Inulinase; Central composite design; Response surface methodology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300911
Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil
Provedor de dados: 58
Autores: Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto
Fernandes.
The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located
in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from
individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in
18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk
samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic
heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential
transmission of...
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018
Hypocholesterolemic effects of Kluyveromyces marxianus M3 isolated from Tibetan mushrooms on
diet-induced hypercholesterolemia in rat
Provedor de dados: 58
Autores: Xie,Yuanhong; Zhang,Hongxing; Liu,Hui; Xiong,Lixia; Gao,Xiuzhi; Jia,Hui; Lian,Zhengxing; Tong,Nengsheng;
Han,Tao.
To investigate the effects of Kluyveromyces marxianus M3 isolated from Tibetan mushrooms on diet-induced
hypercholesterolemia in rats, female Wistar rats were fed a high-cholesterol diet (HCD) for 28 d to generate hyperlipidemic
models. Hyperlipidemic rats were assigned to four groups, which were individually treated with three different dosages of K.
marxianus M3+HCD or physiological saline+HCD via oral gavage for 28 d. The total cholesterol (TC), triglycerides (TG),
high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels in the serum and liver of
the rats were measured using commercially available enzyme kits. In addition, the liver morphology was also examined using
hematoxylin and eosin staining and optical...
Tipo: Journal article
Palavras-chave: Kluyveromyces marxianus; Hypercholesterolemia; High-cholesterol diet.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200389
Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil
Provedor de dados: 58
Autores: Lima,Lorena A.; Cruz Filho,Raimundo F.; Santos,Januário G. dos; Silva,Wilson C.
Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic
activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point
was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on
the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production
conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed
optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C)...
Tipo: Journal article
Palavras-chave: Collagenolytic protease; Bacillus sp; Factorial design; Amazonian soil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401217
In vitro antagonistic growth effects of Lactobacillus fermentum and Lactobacillus salivarius and their
fermentative broth on periodontal pathogens
Provedor de dados: 58
Autores: Chen,Ling-Ju; Tsai,Hsiu-Ting; Chen,Wei-Jen; Hsieh,Chu-Yang; Wang,Pi-Chieh; Chen,Chung-Shih; Wang,Lina;
Yang,Chi-Chiang.
As lactobacilli possess an antagonistic growth property, these bacteria may be beneficial as bioprotective agents for infection
control. However, whether the antagonistic growth effects are attributed to the lactobacilli themselves or their fermentative broth
remains unclear. The antagonistic growth effects of Lactobacillus salivarius and Lactobacillus fermentum as well as their
fermentative broth were thus tested using both disc agar diffusion test and broth dilution method, and their effects on periodontal
pathogens, including Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalisin vitro at different
concentrations and for different time periods were also compared. Both Lactobacillus salivarius and Lactobacillus fermentum and
their...
Tipo: Journal article
Palavras-chave: Lactobacillus fermentum; Lactobacillus salivarius; Streptococcus mutans; Streptococcus sanguis;
Porphyromonas gingivalis; Periodontitis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400019
Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli,
Salmonella, Listeria monocytogenes and Aeromonas hydrophila
Provedor de dados: 58
Autores: Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.
Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium
cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of
Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all
the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and
Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger
showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and...
Tipo: Journal article
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas
hydrophila.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011
Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their
viability
Provedor de dados: 58
Autores: Winkelströter,Lizziane Kretli; Martinis,Elaine C.P. De.
Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in
food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface,
after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Chain Reaction (qPCR).
Biofilms of L. monocytogenes were obtained in stainless steel coupons immersed in Brain Heart Infusion Broth, under agitation at
37 °C for 24 h. The methods selected for this study were based on plate count, microscopic count with the aid of viability dyes
(CTC-DAPI), and qPCR. Results of culture method showed that peroxyacetic acid was efficient to kill sessile L. monocytogenes...
Tipo: Journal article
Palavras-chave: Biofilms; L. monocytogenes; QPCR; Peroxyacetic acid; Sodium hypochlorite.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100231
DIPHTHERIA IN A VACCINATED ADULT IN RIO DE JANEIRO, BRAZIL
Provedor de dados: 58
Autores: Mattos-Guaraldi,Ana Luiza; Formiga,Luiz Carlos Duarte; Marques,Elizabeth Andrade; Pereira,Gabriela Andrade;
Moreira,Lílian Oliveira; Pimenta,Fabrícia Pires; Camello,Thereza Cristina Ferreira; Oliveira,Elsa Fuchshuber.
In 1999, a case of diphtheria in a 32-year-old woman was reported. The patient developed a sore throat immediately after
participating of a five-day meeting with European workers in Rio de Janeiro. Her history included complete pediatric
immunization (DTP) and three doses of adult formulation tetanus and diphtheria toxoid (dT) two years earlier. Clinical diagnosis
of diphtheria was not made until microbiologic examination of specimens confirmed toxigenicity of Corynebacterium diphtheriae
var. gravis, a biotype currently found circulating within Europe where diphtheria remains epidemic. This case reinforces the
potential susceptibility of Brazilian adults to epidemic diphtheria in the vaccine era.
Tipo: Journal article
Palavras-chave: Corynebacterium diphtheriae; DT immunization; Adult diphtheria patient.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300015
Influence of wearing time on efficacy of disposable surgical masks as microbial barrier
Provedor de dados: 58
Autores: Barbosa,Maria Helena; Graziano,Kazuko Uchikawa.
The present study evaluated the efficacy of disposable surgical masks as a microbial barrier with 95% of Bacteria Filtration
Efficacy (BFE) according to the wearing time (1, 2, 4 and 6 hours). The masks showed a decrease in efficacy after a 4-hour
wearing time.
Tipo: Journal article
Palavras-chave: Surgical masks; Hospital infection; Microbial barrier.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300003
An investigation on in vitro and in vivo antimicrobial properties of the antidepressant: amitriptyline
hydrochloride
Provedor de dados: 58
Autores: Mandal,Anurup; Sinha,Chandrima; Jena,Aditya Kumar; Ghosh,Soma; Samanta,Amalesh.
The antidepressant drug amitriptyline hydrochloride was obtained in a dry powder form and was screened against 253 strains of
bacteria which included 72 Gram positive and 181 Gram negative bacteria and against 5 fungal strains. The minimum inhibitory
concentration (MIC) was determined by inoculating a loopful of an overnight peptone water culture of the organism on nutrient
agar plates containing increasing concentrations of amitriptyline hydrochloride (0, 10 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL,
200 µg/mL). Amitriptyline hydrochloride exhibited significant action against both Gram positive and Gram negative bacteria at
25-200 µg/mL. In the in vivo studies it was seen that amitriptyline hydrochloride at a concentration of 25 µg/g and 30 µg/g body
weight of...
Tipo: Journal article
Palavras-chave: Amitriptyline hydrochloride; Antimicrobial activity; Non antibiotics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300014
Detection of virulence genes in Salmonella Enteritidis isolated from different sources
Provedor de dados: 58
Autores: Oliveira,Sílvia Dias de; Rodenbusch,Carla Rosane; Michael,Geovana B.; Cardoso,Marisa I.R.; Canal,Cláudio Wageck;
Brandelli,Adriano.
The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry,
pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC.
There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results
indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized.
Tipo: Journal article
Palavras-chave: Salmonella Enteritidis; Virulence; Spv; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500042
New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract
Provedor de dados: 58
Autores: Silva,Debora Danielle Virginio da; Cândido,Elisangela de Jesus; Arruda,Priscila Vaz de; Silva,Silvio Silvério da;
Felipe,Maria das Graças de Almeida.
The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of
microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable
medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia)
stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen
sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments
were carried out with hydrolysate supplemented with rice bran extract and (NH4)2SO4; peptone and yeast extract; (NH4)2SO4,...
Tipo: Journal article
Palavras-chave: Candida guilliermondii; Hemicellulosic hydrolysate; Scheffersomyces stipitis; Rice bran extract; Sugarcane
bagasse.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400043
Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
Provedor de dados: 58
Autores: Saxena,Rajshree; Singh,Rajni.
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from
soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g
of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The
optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be
thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and
Co++ enhanced the enzyme activity.
Tipo: Journal article
Palavras-chave: Solid State Fermentation; Agro-industrial wastes; Amylase; Enzyme optimization; Stability; Bacillus sp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
Antibiotic susceptibility profiles of Mycoplasma hominis and Ureaplasma urealyticum isolated during
a population-based study concerning women infertility in northeast Romania
Provedor de dados: 58
Autores: Mihai,Mareş; Valentin,Năstasă; Bogdan,Doroftei; Carmen,Chifiriuc Mariana;
Coralia,Bleotu; Demetra,Socolov.
The study was carried out on 1068 infertile women under initial evaluation. For Mycoplasma hominis, the highest resistance rates
were registered for ciprofloxacin (72.22%), followed by macrolides and ofloxacin. For Ureaplasma urealyticum, the ciprofloxacin
resistance was also high (51.72%), while the resistance rates to other tested antibiotics were significantly lower.
Tipo: Journal article
Palavras-chave: Infertility; Fluoroquinolones resistance; Mycoplasma hominis; Ureaplasma urealyticum.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100032
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification
process using different sources of carbon
Provedor de dados: 58
Autores: Marchetto,Margarida; Gianotti,Eloisa Pozzi; Campos,José Roberto; Pires,Roberto Cleto; Moraes,Elizabeth de Mattos.
A study of the kinetics of denitrification was carried out in the laboratory based on the quantification of N2O, the final product of
the activity of denitrifying microorganisms, when the enzymatic reduction of N2O to N2 was blocked by acetylene. Concentrated
mixed liquor (sludge from a reactor with intermittent aeration used for sewage treatment) was used as the inoculum, while
methanol, acetic acid, glucose, effluent sewage from an anaerobic fluidized bed reactor and synthetic substrate simulating
domestic sewage were used as carbon sources. The mean concentration of nitrate was 20 mg/L. Maxima of N2O production and
NO3- consumption occurred between 0.5h and 2.0h of incubation using all the carbon sources, which characterized the
denitrification process....
Tipo: Journal article
Palavras-chave: Denitrifying activity; Denitrifying bacteria; Denitrification; Tertiary treatment of domestic sewage.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200003
Detection of extended spectrum beta lactamases, ampc beta lactamases and metallobetalactamases in
clinical isolates of ceftazidime resistant Pseudomonas Aeruginosa
Provedor de dados: 58
Autores: Umadevi,Sivaraman; Joseph,Noyal M; Kumari,Kandha; Easow,Joshy M; Kumar,Shailesh; Stephen,Selvaraj;
Srirangaraj,Sreenivasan; Raj,Sruthi.
We studied the prevalence of ceftazidime resistance in Pseudomonas aeruginosa and the rates of extended-spectrum
β-lactamase (ESBL), AmpC β-lactamase (AmpC) and metallo-β-lactamase (MBL) production
among the ceftazidime resistant Pseudomonas aeruginosa. A very high rate of MBL production was observed, which suggested it
to be an important contributing factor for ceftazidime resistance among Pseudomonas aeruginosa.
Tipo: Journal article
Palavras-chave: Extended-spectrum β -lactamases AmpC metallo-β -lactamases ceftazidime resistance
Pseudomonas aeruginosa.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400006
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of
vaginal lactobacilli in isolates
Provedor de dados: 58
Autores: Brolazo,Eliane Melo; Leite,Domingos Silva; Tiba,Monique Ribeiro; Villarroel,Marina; Marconi,Camila; Simoes,Jose
Antonio.
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability
of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification
of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical
profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex
polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa
index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii
(25%)...
Tipo: Journal article
Palavras-chave: Carbohydrate fermentation test; Multiplex PCR; Identification; Vaginal lactobacilli; Vaginal ecosystem.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028
Field persistence of Bacillus thuringiensis on maize leaves (Zea mays L.)
Provedor de dados: 58
Autores: Haddad,Marinéia de Lara; Polanczyk,Ricardo Antonio; Alves,Sergio Batista; Garcia,Marcelo de Oliveira.
The persistence slope of Bacillus thuringiensis (Bt) based products in the field is an important parameter to evaluate their efficacy.
The half-life, estimated based on persistence slope parameters, is one of the most effective tools to select microbial pesticides. The
aim of this research was to study the relationship between viability loss of Bt spores on maize leaves and their concentration,
comparing it with field persistence. The experimental design was split-plot on time, composed by maize plants, in which three
concentrations (half, normal and double doses) of a Dipel commercial formulation were applied. In each plot three leaves in the
upper part of three plants where randomly selected. Samples of these leaves were collected 3 to 72 hours after...
Tipo: Journal article
Palavras-chave: Entomopathogen; Dipel; Maize leaves; Bacillus thuringiensis; Field persistence.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400001
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs
with urinary tract infections
Provedor de dados: 58
Autores: Maluta,Renato Pariz; Stella,Ariel Eurides; Riccardi,Kátia; Rigobelo,Everlon Cid; Marin,José Moacir;
Carvalho,Marileda Bonafim; Ávila,Fernando Antonio de.
Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and
companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these
strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H
antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and
genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial
that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%),...
Tipo: Journal article
Palavras-chave: Biomarkers; Biotyping; ERIC-PCR; UPEC.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100045
First isolation of leptospires from dairy goats in Brazil
Provedor de dados: 58
Autores: Lilenbaum,Walter; Morais,Zenaide M.; Gonçales,Amane Paldês; Souza,Gisele O. de; Richtzenhain,Leonardo;
Vasconcellos,Silvio A.
Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro
and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive
reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%),
Shermani (30.8%), Icterohaemorrhagiae (9.6%), Grippotyphosa (9.6%), Autumnalis (5.8%), Castellonis (3.8%) and Bratislava
(3.8%). Two strains of Leptospira sp. were isolated, both in the same region, but from different flocks. Presumptive identification
based on serologic methods suggests those strains to be from Grippotyphosa serogroup.
Tipo: Journal article
Palavras-chave: Goats; Leptospirosis; Leptospira sp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300023
Quantification and molecular characterization of Salmonella isolated from food samples involved in
salmonellosis outbreaks in Rio Grande do Sul, Brazil
Provedor de dados: 58
Autores: Mürmann,Lisandra; Santos,Maria Cecília dos; Longaray,Solange Mendes; Both,Jane Mari Corrêa; Cardoso,Marisa.
Data concerning the prevalence and populations of Salmonella in foods implicated in outbreaks may be important to the
development of quantitative microbial risk assessments of individual food products. In this sense, the objective of the present
study was to assess the amount of Salmonella sp. in different foods implicated in foodborne outbreaks in Rio Grande do Sul
occurred in 2005 and to characterize the isolated strains using phenotypic and genotypic methods. Nineteen food samples involved
in ten foodborne outbreaks occurred in 2005, and positive on Salmonella isolation at the Central Laboratory of the Health
Department of the State of Rio Grande do Sul, were included in this study. Food samples were submitted to estimation of
Salmonella using the Most...
Tipo: Journal article
Palavras-chave: Salmonella; Foodborne outbreak; Quantification; PFGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300024
Screening for antimicrobial activity among bacteria isolated from the Amazon Basin
Provedor de dados: 58
Autores: Motta,Amanda S.; Cladera-Olivera,Florencia; Brandelli,Adriano.
Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon
Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was
mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances
produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed
thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to
pronase E at 2 mg ml-1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels...
Tipo: Journal article
Palavras-chave: Antimicrobial activity; Listeria monocytogenes; Bacteriocins; Amazon Basin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300007
Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS
754.68
Provedor de dados: 58
Autores: Samadlouie,Hamid-Reza; Hamidi-Esfahani,Zohreh; Alavi,Seyed-Mehdi; Varastegani,Boshra.
The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes
involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the
arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred
presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently
compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The
results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the
early...
Tipo: Journal article
Palavras-chave: Mortierella alpina; Arachidonic acid; Lipid; Gene expression; Real time PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010
A COMPARATIVE STUDY OF BACTERIOSTATIC ACTIVITY OF SYNTHETIC
HYDROXYLATED FLAVONOIDS
Provedor de dados: 58
Autores: Olivella,Mónica S.; Zarelli,Valeria E.P.; Pappano,Nora B.; Debattista,Nora B.
Among other properties, flavonoids present a notable bacteriostatic activity. In this paper, minimal inhibitory concentrations
(MICs) of 5,7,4'-trihydroxyflavanone (naringenin), 5,7-dihydroxyflavone and 2',4',4- trihydroxychalcone (isoliquitirigenin)
against Staphylococcus aureus ATCC 25 923 were determined and compared to values obtained for other chalcones and
flavanones previously investigated. Specific growth rates and MICs were determined by a turbidimetric kinetic method. The
observed sequence MICflavanone (inactive) >MIC7-hidroxyflavanone (197.6 µgml-1)>MIC5,7,4'-trihydroxyflavanone
(120 µgml-1) showed that the introduction of an electron donating group (-OH) causes an increase in bioactivity. On the other
hand, the comparisons...
Tipo: Journal article
Palavras-chave: Flavanone; Flavone; Chalcone; Bacteriostatic activity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300013
Kinetic properties of Streptomyces canarius L- Glutaminase and its anticancer efficiency
Provedor de dados: 58
Autores: Reda,Fifi M.
Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It
has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase
showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide
range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for
L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase
showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets
after 10 days...
Tipo: Journal article
Palavras-chave: L-glutaminase; Anti-cancer; Cytotoxicity; MTT assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400957
2(5H)-Furanone: a prospective strategy for biofouling-control in membrane biofilm bacteria by
quorum sensing inhibition
Provedor de dados: 58
Autores: Ponnusamy,Kannan; Paul,Diby; Kim,Young Sam; Kweon,Ji Hyang.
Biofouling of membranes demands costly periodic cleaning and membrane replacement. A sustainable and environmentally
friendly solution for maintenance is not available and would be of great interest for many purposes including economical. As
complex biofilm formation by environmental strains is the major cause of biofouling and biofilm formation in most cases are
controlled by N-Acylhomoserine lactone (AHL)mediated Quorum Sensing (QS). An effort was made to understand the
appropriateness of 2(5H)-furanone, to use against biofouling of membranes. QS inhibition activity by 2(5H)-furanone was studied
using bioindicator strains and known AHLs of different acyl chain lengths. The biofilm inhibition was studied by growth analysis
on polystyrene plate of...
Tipo: Journal article
Palavras-chave: Furanone; Biofilm; Water treatment; Quorum sensing; N-Acyl homoserine lactone.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100032
Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes
Provedor de dados: 58
Autores: Blanco,Alina Sánchez; Durive,Osmar Palacios; Pérez,Sulema Batista; Montes,Zoraida Díaz; Guerra,Nelson Pérez.
ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery
wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions
(T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for
high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under
these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was
achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained
after 15 h of incubation...
Tipo: Journal article
Palavras-chave: Amylases; Bacillus subtilis; Brewery wastes; Proteases; Response surface methodology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300665
Occurrence of adhesin-encoding operons in Escherichia coli isolated from breeders with salpingitis
and chicks with omphalitis
Provedor de dados: 58
Autores: Knöbl,Terezinha; Gomes,Tânia A. Tardelli; Vieira,Mônica A. Midolli; Bottino,José A.; Ferreira,Antonio J. Piantino.
The occurrence of fim, pap and sfa operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis
was evaluated. Analysis of 100 E. coli isolates, by colony hybridization tests, showed that 78 (78%) were fim+, one (1%) was
sfa+, seven (7%) were fim+ associated with pap+, eigth (8%) were fim+ and sfa+, one (1%) was fim+pap+sfa+ and five (5%)
isolates did not hybridize with any probe. These results suggest that fim adhesion-encoding operon plays an important role in
pathogenesis of E. coli infection in chickens with salpingitis and omphalitis.
Tipo: Journal article
Palavras-chave: Escherichia coli; Adhesion-encoding operon; Salpingitis; Omphalitis; Poultry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200008
Employing pharmacokinetic and pharmacodynamic principles to optimize antimicrobial treatment in
the face of emerging resistance
Provedor de dados: 58
Autores: Santos Filho,Lauro; Kuti,Joseph L.; Nicolau,David P.
Antimicrobial efficacy in vivo is not exclusively defined by the activity of an antibiotic as determined in the in vitro susceptibility
test. Knowledge of the pharmacokinetics and pharmacodynamics of antimicrobials and all phenomena occurring between
antimicrobial agents and microorganisms is imperative. The pharmacodynamic (PD) parameters most often used in studies of
antibiotic effect include the following relationships: the maximum free concentration (fCmax) to minimum inhibitory
concentration (MIC) ratio, the free area under the curve (fAUC/MIC) ratio and the duration of time the free concentration exceeds
the MIC (fT>MIC). Utilization of known pharmacokinetic/ pharmacodynamic surrogate relationships should help to optimize
treatment outcome,...
Tipo: Journal article
Palavras-chave: Antibiotic; Bacteria; Pharmacodynamics; Resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200001
Activity of passion fruit (Passiflora edulis) and guava (Psidium guajava) pulps on Lactobacillus
acidophilus in refrigerated mousses
Provedor de dados: 58
Autores: Buriti,Flávia C. A.; Komatsu,Tiemy R.; Saad,Susana M.I.
Five pilot-scale mousse-making trials were produced and supplemented with Lactobacillus acidophilus La-5 probiotic culture: M1
with passion fruit concentrated juice (PJ); M2 with PJ and prebiotic fibre inulin; M3 with frozen passion fruit pulp (PP); M4 with
frozen guava pulp (GP); M5 with GP and lactic acid. The products were stored refrigerated (4ºC) and M2 and M5 were also stored
frozen (-18ºC). Viability of L. acidophilus decreased up to 4.7 log cfu.g-1 in the 21st day for refrigerated mousses containing
passion fruit (M1, M2 and M3), whereas the probiotic population remained above 6 log cfu.g-1 in the mousses containing guava
(M4 and M5). Inhibition due to acidity was discharged. The addition of fruits to probiotic products should be carefully planned...
Tipo: Journal article
Palavras-chave: Lactobacillus acidophilus; Passiflora edulis; Psidium guajava; Mousse; Refrigeration; Freezing.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200025
Bioethanol production from rice straw residues
Provedor de dados: 58
Autores: Belal,Elsayed B.
A rice straw -cellulose utilizing mold was isolated from rotted rice straw residues. The efficient rice straw degrading
microorganism was identified as Trichoderma reesei. The results showed that different carbon sources in liquid culture such as
rice straw, carboxymethyl cellulose, filter paper, sugar cane bagasse, cotton stalk and banana stalk induced T. reesei cellulase
production whereas glucose or Potato Dextrose repressed the synthesis of cellulase. T. reesei cellulase was produced by the solid
state culture on rice straw medium. The optimal pH and temperature for T. reesei cellulase production were 6 and 25 ºC,
respectively. Rice straw exhibited different susceptibilities towards cellulase to their conversion to reducing sugars. The present
study...
Tipo: Journal article
Palavras-chave: Cellulase; Trichoderma reesei; Rice straw residues; Enzymatic hydrolysis; Bioethanol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100033
Salmonellosis in Rio Grande do Sul, Brazil, 1997 to 1999
Provedor de dados: 58
Autores: Costalunga,Suzana; Tondo,Eduardo Cesar.
Salmonella spp. was the major cause of reported foodborne diseases in the last years in the State of Rio Grande do Sul, South of
Brazil. Epidemiological data on salmonellosis, occurred in the period of 1997 to 1999 and supplied by the Division of Sanitary
Surveillance (DVS/RS), were analysed according to the following factors: total number of confirmed outbreaks, number of people
involved, outbreaks incidence according to the period of the year, age and sex of involved people, food vehicle, food storage
conditions, local where the disease occurred, and possible causes of the outbreaks. The results indicated that 8217 people were
involved, and 1557 had to be hospitalized. The highest number of outbreaks occurred during springtime and the principal age
group...
Tipo: Journal article
Palavras-chave: Spp.; Foodborne disease; Salmonellosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400013
Subtyping of Chilean Methicillin-Resistant Staphylococcus aureus strains carrying the staphylococcal
cassette chromosome mec type I
Provedor de dados: 58
Autores: Medina,Gustavo; Otth,Carola; Otth,Laura; Fernández,Heriberto; Muñoz,Celeste; Cruz,María; Zaror,Ángela;
Henriquez,Ruby; Arce,Maria; Wilson,Myra.
The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential
components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J
regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec
type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec
type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were
analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene.
MRSA strains were classified into seventeen...
Tipo: Journal article
Palavras-chave: Subtypified MRSA; Polymorphism MRSA; SCCmec MRSA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300006
Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions
Provedor de dados: 58
Autores: Yamanaka,Renata; Soares,Clarissa F.; Matheus,Dácio R.; Machado,Kátia M.G.
The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics,
mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven
biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The
influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of
manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase
activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within
the first...
Tipo: Journal article
Palavras-chave: Laccase; Metals; MnP; Vegetable oil; Surfactants.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100019
Immobilized Saccharomyces cerevisiae as a potential aflatoxin decontaminating agent in pistachio
nuts
Provedor de dados: 58
Autores: Rahaie,S.; Emam-Djomeh,Z.; Razavi,S. H.; Mazaheri,M.
In this study, we investigated the binding ability of Saccharomayces cerevisiae to aflatoxin in pistachio nuts. The obtained results
indicate that S. cerevisiae has an aflatoxin surface binding ability of 40% and 70% (with initial aflatoxin concentrations of 10 and
20 ppb) in the exponential phase. Acid treatments increase this ability to approximately 60% and 73% for the two concentrations
of aflatoxin, respectively. Heat treatments also enhance surface binding to 55% and 75%, respectively. Binding appears to be a
physical phenomenon that saturates within the first 2-3 hours of the process. The obtained results indicate that yeast
immobilization for toxin reduction on aflatoxin-contaminated pistachios had no effect on qualitative characteristics, such as...
Tipo: Journal article
Palavras-chave: Aflatoxin; Pistachio nut; Saccharomayces cerevisiae; Surface binding.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100014
Immunological assays employed for the elucidation of an histoplasmosis outbreak in São Paulo, SP
Provedor de dados: 58
Autores: Passos,Angela Noronha; Kohara,Valdelene Sayuri; Freitas,Roseli Santos de; Vicentini,Adriana Pardini.
Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or
researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the
histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients
with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the
cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum
examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions
in...
Tipo: Journal article
Palavras-chave: Histoplasma; Histoplasmosis; Immunoassay; Double immunodiffusion; Immunoblotting.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400028
Utilization of agroindustrial residues for lipase production by solid-state fermentation
Provedor de dados: 58
Autores: Damaso,Mônica Caramez Triches; Passianoto,Moisés Augusto; Freitas,Sidinéa Cordeiro de; Freire,Denise Maria
Guimarães; Lago,Regina Celi Araujo; Couri,Sonia.
The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue
supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first
steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the
Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two
substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with
wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated
as...
Tipo: Journal article
Palavras-chave: Lipase; Solid state fermentation; Agroindustrial residues; Corn oil by-products.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400015
Dominant culturable bacterial microbiota in the digestive tract of the American black vulture
(Coragyps atratus Bechstein 1793) and search for antagonistic substances
Provedor de dados: 58
Autores: Carvalho,Lydston Rodrigues de; Farias,Luiz Macêdo; Nicoli,Jacques Robert; Silva,Maria Clara Fernandes;
Corsino,Andrea Teresa Santos Meira; Lima,Luiz André de; Redondo,Rodrigo Aparecido Fernandes; Ferreira,Paulo César
Peregrino; Pinto,Maria Elizabeth Bernardes Margutti.
Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus
Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate
possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted
to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for
enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them.
The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37...
Tipo: Journal article
Palavras-chave: Culture; Microbiota; Digestive tract; Antagonistic substance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300007
Regulation of Saccharomyces cerevisiae maltose fermentation by cold temperature and CSF1
Provedor de dados: 58
Autores: Hollatz,Claudia; Stambuk,Boris Ugarte.
We studied the influence of cold temperature (10ºC) on the fermentation of maltose by a S. cerevisiae wild-type strain, and a
csf1delta mutant impaired in glucose and leucine uptake at low temperatures. Cold temperature affected the fermentation kinetics
by decreasing the growth rate and the final cell yield, with almost no ethanol been produced from maltose by the wild-type cells at
10ºC. The csf1delta strain did not grew on maltose when cultured at 10ºC, indicating that the CSF1 gene is also required for
maltose consumption at low temperatures. However, this mutant also showed increased inhibition of glucose and maltose
fermentation under salt stress, indicating that CSF1 is probably involved in the regulation of other physiological processes,
including...
Tipo: Journal article
Palavras-chave: Refrigerated dough; Maltose fermentation; Baker's yeast; Salt stress.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500034
Media components and amino acid supplements influencing the production of fruity aroma by
Geotrichum candidum
Provedor de dados: 58
Autores: Pinotti,T.; Carvalho,P.M.B.; Garcia,K.M.G.; Silva,T.R.; Hagler,Allen Norton; Leite,S.G.F.
The ability of Geotrichum candidum to produce fruity aroma in food grade sucrose, molasses, corn steep liquor and peptone based
culture media was tested by sensory evaluation and analyzed by gas chromatography mass spectrometry. A strong and sweet
fruity aroma was produced from molasses, with peptone or corn steep liquor stimulating aroma production. Molasses with peptone
supplemented with leucine, valine, or alanine yielded better fruity aroma production and the presence of many esters was
consistent with the fruity aroma production.
Tipo: Journal article
Palavras-chave: Geotrichum candidum; Aroma; Corn steep liquor; Molasses; Amino acids.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400017
Model of experimental infection in healthy and immunosuppressed swiss albino mice (Mus musculus)
using Candida albicans strains with different patterns of enzymatic activity
Provedor de dados: 58
Autores: Chaves,Guilherme M.; Cavalcanti,Maria Auxiliadora de Q.; Carneiro-Leão,Ana Maria dos A.; Lopes,Silvia L.
With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and
proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the
URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL-1) activity. The
other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase
(Pz=0.482) and proteinase (0.780 U.mL-1) activity. The strains were inoculated via intraperitoneum in immunosuppressed and
non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and
kidneys were...
Tipo: Journal article
Palavras-chave: Candida albicans; Extracellular enzymes; Virulence; Experimental infection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300010
Microbiological biodiversity in poultry and paddy straw wastes in composting systems
Provedor de dados: 58
Autores: Devi,Sunita; Sharma,C.R; Singh,Kamlesh.
Immense quantity of waste is generated in association with poultry meat egg and crop production. The potential risks due to
disposal of these wastes are magnified as a result of dense refinement of poultry production and the decreasing amount of land
available for waste disposal. The study aims at studying the microbiological biodiversity of poultry waste and paddy straw based
co-composting system. The predominant microflora of the poultry manure were bacteria, fungi, enteric bacteria and spore forming
bacteria whose population was high at the initiation of composting but decreased significantly as the compost approached
maturity. The initial load of inherent enteric groups of bacteria in poultry waste, that also includes some pathogenic ones, is...
Tipo: Journal article
Palavras-chave: Composting; Microbial succession; Organic waste; Microbiological biodiversity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100034
Methods to preserve potentially toxigenic fungi
Provedor de dados: 58
Autores: Guimarães,Lucas Costa; Fernandes,Ana Paula; Chalfoun,Sara Maria; Batista,Luís Roberto.
Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and
animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained
characteristic is of paramount importance, so the process does not need to be repeated. Fungi are eukaryotic, achlorophyllous,
heterotrophic organisms, usually filamentous, absorb their food, can be either macro or microscopic, propagate themselves by
means of spores and store glycogen as a source of storage. Fungi, while infesting food, may produce toxic substances such as
mycotoxins. The great genetic diversity of the Kingdom Fungi renders the preservation of fungal cultures for many years relevant.
Several...
Tipo: Journal article
Palavras-chave: Mycotoxins; Toxigenic fungi; Methods for fungal conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100007
The use of standard methodology for determination of antifungal activity of natural products against
medical yeasts Candida sp and Cryptococcus sp
Provedor de dados: 58
Autores: Scorzoni,Liliana; Benaducci,Tatiane; Almeida,Ana Marisa Fusco; Silva,Dulce Helena Siqueira; Bolzani,Vanderlan da
Silva; Gianinni,Maria José Soares Mendes.
Methodology for testing natural compounds for determination of antifungal activity had been developed with adaptations. The
most used are bioautography and agar diffusion with a complex and no defined media. In this study, different methods for
determination of antifungal activity of natural products are discussed and the use of M27-A2 microdilution test from CLSI
(Clinical and Laboratory Standards Institute, 2002), as general standard methodology for testing plant extracts activity is
recommended.
Tipo: Journal article
Palavras-chave: Natural products; Antifungal activity; Candida sp; Cryptococcus sp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300001
Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from
India
Provedor de dados: 58
Autores: Agoramoorthy,G.; Chandrasekaran,M.; Venkatesalu,V.; Hsu,M.J.
Fatty acids are widely occurring in natural fats and dietary oils and they are known to have antibacterial and antifungal properties.
However, little is known on the antibacterial and antifungal properties of the blind-your-eye mangrove (Excoecaria agallocha) and
this study for the first time determines the fatty acid composition and the antibacterial and antifungal activities of Fatty Acid
Methyl Esters (FAME) of the blind-your-eye mangrove plant found along the coastal areas of south India.
Tipo: Journal article
Palavras-chave: Blind-your-eye mangrove; Excoecaria agallocha; Antibacterial activity; Antifungal activity; Fatty acid methyl
esters.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400028
Physiological studies on carboxymethyl cellulase formation by Aspergillus terreus DSM 826
Provedor de dados: 58
Autores: Abdel-Fatah,Osama M.; Hassan,Mohamed M.; Elshafei,Ali M.; Haroun,Bakry M.; Atta,Housam M.;
Othman,Abdelmageed M.
Physiological studies were conducted to determine the optimum cultural conditions for maximal carboxymethyl cellulase
(CMCase) formation by Aspergillus terreus DSM 826. Shaking condition at 150 rpm is favorable for the production of CMCase
from rice straw and sugar cane bagasse. The highest enzyme yield was obtained at the third day of incubation at 30ºC for both
cases; however CMCase formation occurred at a broad range of pH values, with maximal formation of A. terreus DSM 826
CMCase at pH 4.5 and 5.0 when rice straw and sugar cane bagasse were used as sole carbon source, respectively. Carboxymethyl
cellulose (CMC) was found to be a good inducer for CMCase formation in both agricultural wastes with CMC concentrations of
0.5 and 1.0 % (w/v) in case of rice...
Tipo: Journal article
Palavras-chave: Aspergillus terreus; Carboxymethyl cellulase (CMCase); Rice straw; Sugar cane bagasse.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100001
Antifungal activity of topical microemulsion containing a thiophene derivative
Provedor de dados: 58
Autores: Guimarães,Geovani Pereira; Reis,Mysrayn Yargo de Freitas Araújo; Silva,Dayanne Tomaz Casimiro da; Mendonça
Junior,Francisco Jaime Bezerra; Converti,Attílio; Pessoa Jr,Adalberto; Damasceno,Bolívar Ponciano Goulart de Lima; Silva,José
Alexsandro da.
Fungal infections have become a major problem of worldwide concern. Yeasts belonging to the Candida genus and the pathogenic
fungus Cryptococcus neoformans are responsible for different clinical manifestations, especially in immunocompromised patients.
Antifungal therapies are currently based on a few chemotherapeutic agents that have problems related to effectiveness and
resistance profiles. Microemulsions are isotropic, thermodynamically stable transparent systems of oil, water and surfactant that
can improve the solubilization of lipophilic drugs. Taking into account the need for more effective and less toxic drugs along with
the potential of thiophene derivatives as inhibitors of pathogenic fungi growth, this study aimed to evaluate the antifungal...
Tipo: Journal article
Palavras-chave: Antifungal susceptibility; Cryptococcus neoformans; Microemulsion; Thiophene derivatives.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200024
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides
brasiliensis
Provedor de dados: 58
Autores: Bianchini,A.A.C.; Petroni,T.F.; Fedatto,P.F.; Bianchini,R.R.; Venancio,E.J.; Itano,E.N.; Ono,M.A.
The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous
disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector
component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the
dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken
and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to
activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs
and the...
Tipo: Journal article
Palavras-chave: Paracoccidioidomycosis; Dog; Complement.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005
Expression of virulence genes by Listeria monocytogenes J0161 in natural environment
Provedor de dados: 58
Autores: Tirumalai,Prem Saran; Prakash,Soam.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our
objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are
different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the
expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a
saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other
virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different
from...
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; PrfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200050
Epicoccum nigrum and Cladosporium sp. for the treatment of oily effluent in an air-lift reactor
Provedor de dados: 58
Autores: Queissada,Daniel Delgado; Silva,Flávio Teixeira da; Penido,Juliana Sundfeld; Siqueira,Carolina Dell'Aquila;
Paiva,Tereza Cristina Brazil de.
The metalworking industry is responsible for one of the most complex and difficult to handle oily effluents. These effluents
consist of cutting fluids, which provide refrigeration and purification of metallic pieces in the machining system. When these
effluents are biologically treated, is important to do this with autochthonous microorganisms; the use of these microorganisms
(bioaugmentation) tends to be more efficient because they are already adapted to the existing pollutants. For this purpose, this
study aimed to use two indigenous microorganisms, Epicoccum nigrum and Cladosporium sp. for metalworking effluent treatment
using an air-lift reactor; the fungus Aspergillus niger (laboratory strain) was used as a reference microorganism. The original...
Tipo: Journal article
Palavras-chave: Epicoccum nigrum; Cladosporium sp.; Metalworking effluent; Air-lift reactor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200041
Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain
Provedor de dados: 58
Autores: Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva;
Paroul,Natália; Luccio,Marco Di; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen.
Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions.
After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated,
'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty
acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP'
enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their
activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary...
Tipo: Journal article
Palavras-chave: Sporidiobolus pararoseus; 'synthetic lipase'; Submerged fermentation; Enzyme characterization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100043
Kinetic characterization of glucose aerodehydrogenase from Aspergillus niger EMS-150-F after
optimizing the dose of mutagen for enhanced production of enzyme
Provedor de dados: 58
Autores: Umbreen,Huma; Zia,Muhammad Anjum; Rasul,Samreen.
In the present study enhanced production of glucose aerodehydrogenase from Aspergillus niger has been achieved after
optimizing the dose of chemical mutagen ethyl methane sulfonate (EMS) that has not been reported earlier. Different doses of
mutagen were applied and a strain was developed basing upon the best production. The selected strain Aspergillus niger
EMS-150-F was optimized for nutrient requirements in order to produce enzyme through fermentation and the results showed the
best yield at 2% corn steep liquor (CSL), 36 hours fermentation time, pH 5, 30°C temperature, 0.3% KH2PO4, 0.3% urea and
0.06% CaCO3. The enzyme was then purified and resulted in 57.88 fold purification with 52.12% recovery. On kinetic
characterization, the enzyme showed optimum...
Tipo: Journal article
Palavras-chave: Glucose aerodehydogenase; Mutagenesis; Production; Purification; Thermal stability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400012
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
Provedor de dados: 58
Autores: Aguiar,Daniel M.; Hagiwara,Mitika K.; Labruna,Marcelo B.
An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo
Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial
genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains,
respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28
partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis
strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is
the...
Tipo: Journal article
Palavras-chave: Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
Quick stimulation of Alcanivorax sp. by bioemulsificant EPS2003 on microcosm oil spill simulation
Provedor de dados: 58
Autores: Cappello,Simone; Genovese,Maria; Denaro,Renata; Santisi,Santina; Volta,Anna; Bonsignore,Martina;
Mancini,Giuseppe; Giuliano,Laura; Genovese,Lucrezia; Yakimov,Michail M.
Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on
quick stimulation of hydrocarbonoclastic bacteria. Early hours of oil spill, were stimulated using an experimental seawater
microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003); this system was monitored for 2 days and compared
to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and
hydrocarbon-degrading bacteria were carried out. Community composition of marine bacterioplankton was determined by 16S
rRNA gene clone libraries. Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial
abundance and, in particular,...
Tipo: Journal article
Palavras-chave: Alcanivorax; Bioemulsificant exopolysaccharide (EPS2003); Oil spill.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400023
Distribution, detection of enterotoxigenic strains and antimicrobial drug susceptibility patterns of
Bacteroides fragilis group in diarrheic and non-diarrheic feces from Brazilian infants
Provedor de dados: 58
Autores: Ferreira,Débora Paula; Silva,Vânia Lúcia; Guimarães,Danielle Aparecida; Coelho,Cíntia Marques; Zauli,Danielle Alves
Gomes; Farias,Luiz Macêdo; Carvalho,Maria Auxiliadora Roque; Diniz,Claudio Galuppo.
Despite the importance of gastrointestinal diseases and their global distribution, affecting millions of individuals around the world,
the role and antimicrobial susceptibility patterns of anaerobic bacteria such as those in the Bacteroides fragilis group (BFG) are
still unclear in young children. This study investigated the occurrence and distribution of species in the BFG and enterotoxigenic
strains in the fecal microbiota of children and their antimicrobial susceptibility patterns. Diarrheic (n=110) and non-diarrheic
(n=65) fecal samples from children aged 0-5 years old were evaluated. BFG strains were isolated and identified by conventional
biochemical, physiological and molecular approaches. Alternatively, bacteria and enterotoxigenic strains were...
Tipo: Journal article
Palavras-chave: Bacteroides fragilis group; ETBF; Antimicrobial susceptibility; Diarrhea.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300010
Comparative growth of trichoderma strains in different nutritional sources, using bioscreen c
automated system
Provedor de dados: 58
Autores: Rossi-Rodrigues,Bianca Caroline; Brochetto-Braga,Márcia Regina; Tauk-Tornisielo,Sâmia Maria; Carmona,Eleonora
Cano; Arruda,Valeska Marques; Chaud Netto,José.
Trichoderma is one of the fungi genera that produce important metabolites for industry. The growth of these organisms is a
consequence of the nutritional sources used as also of the physical conditions employed to cultivate them. In this work, the
automated Bioscreen C system was used to evaluate the influence of different nutritional sources on the growth of Trichoderma
strains (T. hamatum, T. harzianum, T. viride, and T. longibrachiatum) isolated from the soil in the Juréia-Itatins Ecological Station
(JIES), São Paulo State - Brazil.The cultures were grown in liquid culture media containing different carbon- (2%; w/v) and
nitrogen (1%; w/v) sources at 28ºC, pH 6.5, and agitated at 150 rpm for 72 h. The results showed, as expected, that glucose is...
Tipo: Journal article
Palavras-chave: Bioscreen C system; Growth; Trichoderma; Tryptone; Yeast extract.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200035
Candida glabrata among Candida spp. from environmental health practitioners of a Brazilian
Hospital
Provedor de dados: 58
Autores: Savastano,Catarina; Silva,Elisa de Oliveira; Gonçalves,Lindyanne Lemos; Nery,Jéssica Maria; Silva,Naiara Chaves;
Dias,Amanda Latercia Tranches.
Abstract The incidence of the species Candida albicans and non-albicans Candida was evaluated in a Brazilian Tertiary Hospital
from the environment and health practitioners. In a 12-month period we had a total positivity of 19.65% of Candida spp. The most
recurring non-albicans Candida species was C. glabrata (37.62%), generally considered a species of low virulence, but with a
higher mortality rate than C. albicans. Subsequently, C. parapsilosis (25.74%) and C. tropicalis (16.86%) were the second and
third most commonly isolated species. Considering the total samples collected from the emergency room and from the inpatient
and the pediatric sector, 19.10% were positive for Candida spp., with the predominance of non-albicans Candida species
(89.42%). The...
Tipo: Journal article
Palavras-chave: Candida glabrata; Candida spp.; Non-albicans Candida species; Hospital environment; Antifungal drugs.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200367
Production, purification and characterization of L-asparaginase from Streptomyces gulbargensis
Provedor de dados: 58
Autores: Amena,S.; Vishalakshi,N.; Prabhakar,M.; Dayanand,A.; Lingappa,K.
L-asparaginase is an anti-neoplastic agent used in the lymphoblastic leukaemia chemotherapy. In the present study a novel strain,
Streptomyces gulbargensis was explored for the production of extra-cellular L-asparaginase using groundnut cake extract. The
optimum pH, temperature, inoculum size and agitation speed for enzyme production were pH 8.5, 40ºC, 1x10(8)spores/ml and
200 rev/min respectively. Maltose (0.5%) and L-asparagine (0.5%) proved to be the best carbon and nitrogen sources respectively.
The enzyme was purified 82.12 fold and the apparent molecular weight of the enzyme was found to be 85 kDa. The optima pH
and temperature for the enzyme were 9.0 and 40ºC respectively. The enzyme was more stable at the alkaline pH than at the acidic
one and it...
Tipo: Journal article
Palavras-chave: L-asparaginase; Streptomyces gulbargensis; Groundnut cake extract; Optimization; Purification.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100025
Resistance of Klebsiella Pneumoniae clinical isolates: linkage of outer membrane proteins (omps) with
production esbls
Provedor de dados: 58
Autores: Marques,Lívia Érika Carlos; Oliveira,Danielle Ferreira de; Marques,Márcia Maria Mendes; Silva,Ana Raquel Araújo
da; Alves,Carlucio Roberto; Guedes,Maria Izabel Florindo.
Three isolates of Klebsiella pneumoniae, collected from the University Hospital in Fortaleza, Brazil, were analyzed to determine
their resistance to multiple antibiotics. The results of this study showed that the resistance of the clinically isolated bacteria is
associated with the production of extended-spectrum beta-lactamases (ESLBs) and loss of outer membrane proteins.
Tipo: Journal article
Palavras-chave: Klebsiella pneumoniae β -lactamase and porin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200009
Symptomatic hydrocephalus in a newborn infected with listeria monocytogenes
Provedor de dados: 58
Autores: Laciar,Analía L.; Hasuoka,Raúl P.; Correa,Silvia M.; Miranda,Alicia M.; Centorbi,Olga N.P.
Central nervous system infections caused by Listeria monocytogenes produce a wide range of clinical symptoms which include
cerebral abscesses, meningitis and nonmeningitic parenchymal cerebritis. A case study is presented of early listeriosis with signs
of meningitis accompanied with septicemia and complicated with severe hydrocephalus.
Tipo: Journal article
Palavras-chave: Listeria monocytogenes; Meningitis; Septicemia; Hydrocephalus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100003
Bacteriophage: laboratorial diagnosis and phage therapy
Provedor de dados: 58
Autores: Silva,Joas L. Da; Hirata,Rosario D.C.; Hirata,Mario H.
Bacteriophages have been researched as a new alternative to antibiotics. These viruses inject their genetic material into bacteria
and use their host machinery to multiply themselves. The research of bacteriophages in Brazil will certainly provide low-cost
treatment of multidrug resistant bacteria, new microbiological diagnosis and advantages for the Brazilian food industry.
Tipo: Journal article
Palavras-chave: Phage-based therapy; Bacterial diseases; Antibiotic resistant bacteria.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300017
Diversity of group A rotavirus genes detected in the Triângulo Mineiro region, Minas Gerais, Brazil
Provedor de dados: 58
Autores: Dulgheroff,Ana Carolina Bernardes; Silva,George Allan Villarouco da; Naveca,Felipe Gomes; Oliveira,Adriana
Gonçalves de; Domingues,André Luiz da Silva.
ABSTRACT Group A rotaviruses are the main causative agent of infantile gastroenteritis. The segmented nature of the viral
genome allows reassortment of genome segments, which can generate genetic variants. In this study, we characterized the
diversity of the VP7, VP4 (VP8*), VP6, NSP4, and NSP5 genes of the rotaviruses that circulated from 2005 to 2011 in the
Triângulo Mineiro (TM) region of Brazil. Samples with genotypes G2 (sublineages IVa-1 and IVa-3), G1 (sublineage I-A), G9
(lineage III), G12 (lineages II and III), G8 (lineage II), G3 (lineage III), P[4] (sublineages IVa and IVb), P[8] (sublineages
P[8]-3.6, P[8]-3.3, and P[8]-3.1), I2 (lineage VII), E2 (lineages VI, XII, and X), and H2 (lineage III) were identified. The
associations found in the...
Tipo: Journal article
Palavras-chave: Rotavirus; Infantile diarrhea; Genotype; Genetic variation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300731
New hosts of Myrothecium spp. in Brazil and a preliminary in vitro assay of fungicides
Provedor de dados: 58
Autores: Quezado Duval,A.M.; Henz,G.P.; Paz-Lima,M.L.; Medeiros,A.R.; Miranda,B.E.C.; Pfenning,L.H.; Reis,A.
Myrothecium roridum and M. verrucaria are two plant pathogenic species causing foliar spots in a large number of cultivated
plants. This paper aims to study the causal agents of foliar spots in vegetable crops (sweet pepper, tomato and cucumber),
ornamental plants (Spathiphyllum wallisii, Solidago canadensis, Anthurium andreanum, Dieffenbachia amoena) and a
solanaceous weed plant (Nicandra physaloides). Most of the isolates were identified as M. roridum; only the isolate 'Myr-02' from
S. canadensis was identified as M. verrucaria. All the isolates were pathogenic to their original plant hosts and also to some other
plants. Some fungicides were tested in vitro against an isolate of M. roridum and the mycelial growth recorded after seven days.
Fungicides...
Tipo: Journal article
Palavras-chave: Myrothecium roridum; M. verrucaria; Vegetable crops; Ornamentals; Weeds; Etiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100034
MONOCLONAL ANTIBODIES TO IDENTIFY TOMATO MOSAIC TOBAMOVIRUS (TOMV)
Provedor de dados: 58
Autores: Duarte,Keila M.R.; Gomes,Luiz Humberto; Gesztesi,Jean-Luc; Lopes,José Daniel; Tavares,Flávio C.A.
Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV) isolated in Brazil. One antibody (8G7G2)
isotyped as IgG2b (kappa light chain) showed strong specificity and very low cross reaction with the Tobacco mosaic virus
(TMV). It can be used in identification of tomato mosaic virus (ToMV).
Tipo: Journal article
Palavras-chave: Tomato mosaic tobamovirus; ELISA; Monoclonal antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300016
The influences of fish infusion broth on the biogenic amines formation by lactic acid bacteria
Provedor de dados: 58
Autores: Küley,Esmeray; Özogul,Fatih; Balikçi,Esra; Durmus,Mustafa; Ayas,Deniz.
The influences of fish infusion decarboxylase broth (IDB) on biogenic amines (BA) formation by lactic acid bacteria (LAB) were
investigated. BA productions by single LAB strains were tested in five different fish (anchovy, mackerel, white shark, sardine and
gilthead seabream) IDB. The result of the study showed that significant differences in ammonia (AMN) and BA production were
observed among the LAB strains in fish IDB (p < 0.05). The highest AMN and TMA production by LAB strains were observed
for white shark IDB. The all tested bacteria had decarboxylation activity in fish IDB. The uppermost accumulated amines by LAB
strains were tyramine (TYM), dopamine, serotonin and spermidine. The maximum histamine production was observed in sardine
(101.69 mg/L)...
Tipo: Journal article
Palavras-chave: Biogenic amines; Lactic acid bacteria; Starter cultures; Fish infusion broth.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200010
The use of ozonized oil in the treatment of dermatophitosis caused by Microsporum canis in rabbits
Provedor de dados: 58
Autores: Daud,Fernanda Vasquez; Ueda,Suely Mitoi Ykko; Navarini,Alessandra; Mímica,Lycia Mara Jenné.
The ozone is effective against most microorganisms due to its high oxidant power. Low concentrations and short-term contact are
sufficient to inactivate bacteria, mold, yeast, parasites, seaweeds, protozoa and fungi. Microsporum canis is an important agent of
dermatophitosis in human and animal. The aim of the current study was to assess the efficacy of ozonized oil over Microsporum
canis in rabbits. Eighteen male New Zealand white rabbits, weight ranging from 2 to 3.2 kg were depilated in the cranial
dorso-lateral and right caudal, and cranial and left caudal regions. The regions were inoculated with Microsporum canis, excepting
the right caudal region, and were denominated TM, O, OM and M, respectively. After seven days, the treatment of lesions in
TM...
Tipo: Journal article
Palavras-chave: Fungi; Microsporum; Oils; Ozone; Rabbits.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100035
Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans
Provedor de dados: 58
Autores: Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira;
Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients
with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much
potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C.
albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type
apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was
determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed
presence of...
Tipo: Journal article
Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004
Survey of molds, yeast and Alicyclobacillus spp. from a concentrated apple juice productive process
Provedor de dados: 58
Autores: Salomão,Beatriz de Cássia Martins; Muller,Chalana; Amparo,Hudson Couto do; Aragão,Gláucia Maria Falcão de.
Bacteria and molds may spoil and/or contaminate apple juice either by direct microbial action or indirectly by the uptake of
metabolites as off-flavours and toxins. Some of these microorganisms and/or metabolites may remain in the food even after
extensive procedures. This study aim to identify the presence of molds (including heat resistant species) and Alicyclobacillus spp.,
during concentrated apple juice processing. Molds were isolated at different steps and then identified by their macroscopic and
microscopic characteristics after cultivation on standard media at 5, 25 and 37ºC, during 7 days. Among the 19 isolated found,
63% were identified as Penicillium with 50% belonging to the P. expansum specie. With regards to heat resistant molds, the
species...
Tipo: Journal article
Palavras-chave: Concentrated apple juice; Alicyclobacillus acidoterrestris; Heat resistant mold; Penicillium expansum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100008
Thymic atrophy and fungal virulence during experimental paracoccidioidomycosis
Provedor de dados: 58
Autores: Brito,Vânia Nieto; Souto,Paula Cristina de Souza; Gameiro,Jacy; Cruz-Höfling,Maria Alice; Verinaud,Liana.
The immunosuppression observed in systemic mycosis can be related to primary lymphoid organs damage. Thus, our laboratory
has studied the effects of the Paracoccidioides brasiliensis infection on the thymus of mice. Here, thymuses of susceptible and
resistant mice were evaluated after inoculation with highly and slightly virulent isolates of the fungus. All groups presented
thymic atrophy, loss of corticomedullary delimitation and increase of apoptotic index. However, mice inoculated with high
virulent strain showed earlier and stronger alterations suggesting that thymic atrophy can be directly related to the fungal virulence
and to the immunosuppression.
Tipo: Journal article
Palavras-chave: Paracoccidioides brasiliensis; Thymus; Fungal virulence.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500005
Bio-preservation of ground beef meat by Enterococcus faecalis CECT7121
Provedor de dados: 58
Autores: Sparo,M.D.; Confalonieri,A.; Urbizu,L.; Ceci,M.; Sánchez Bruni,S.F.
Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main
goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different
pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated
in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat
samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (10³ CFU/g)) and E. faecalis
CECT7121 (10(4) CFU/g) simultaneously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the
pathogens. Study III: E....
Tipo: Journal article
Palavras-chave: Bio-preservation; Probiotic; Ground beef meat; Enterococcus faecalis CECT7121.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100006
Effect of rosemary essential oil and modified-atmosphere packaging (MAP) on meat quality and
survival of pathogens in poultry fillets
Provedor de dados: 58
Autores: Kahraman,Tolga; Issa,Ghassan; Bingol,Enver Baris; Kahraman,Beren Basaran; Dumen,Emek.
The effect of rosemary (Rosmarinus officinalis L.) essential oil (REO) and modified-atmosphere packaging (MAP) on the survival
of certain pathogens (Salmonella Typhimurium and Listeria monocytogenes) in poultry fillets and on their meat quality during 7
days of refrigerated storage were investigated. Because REO at 0.05% and 0.1% had weak antibacterial activity and REO at 0.3%,
0.5% and 1.0% imparted unacceptable organoleptic properties, only REO at 0.2% was used to treat the poultry meat. The results
showed that adding 0.2% REO to poultry fillets did not reduce the size of the population of S. Typhimurium and L.
monocytogenes. However, REO treatment significantly decreased the L* (lightness) value and increased the a* (redness) value of
stored fillets,...
Tipo: Journal article
Palavras-chave: Rosemary; Essential oil; Modified-atmosphere packaging; Poultry; Pathogens.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200591
Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia
calcarata (Haw.) Roscoe
Provedor de dados: 58
Autores: Sunitha.,V. H.; Ramesha.,A.; Savitha.,J.; Srinivas.,C.
Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch
hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries. Considering these facts, endophytic fungi
isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone
agar (GYP) medium. Among thirty isolates of endophytic fungi, isolate number seven identified as Cylindrocephalum sp. (Ac-7)
showed highest amylolytic activity and was taken for further study. Influence of various physical and chemical factors such as pH,
temperature, carbon and nitrogen sources on amylase production in liquid media were studied. The maximal amylase production
was...
Tipo: Journal article
Palavras-chave: Alpinia calcarata Roscoe; Endophytic fungi; Cylindrocephalum sp.; Amylase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300049
Susceptibility testing of Candida albicans isolated from oropharyngeal mucosa of HIV+ patients to
fluconazole, amphotericin B and Caspofungin: killing kinetics of caspofungin and amphotericin B
against fluconazole resistant and susceptible isolates
Provedor de dados: 58
Autores: Lemos,Janine de Aquino; Costa,Carolina Rodrigues; Araújo,Crystiane Rodrigues de; Souza,Lúcia Kioko Hasimoto e;
Silva,Maria do Rosário Rodrigues.
A clear understanding of the pharmacodynamic properties of antifungal agents is important for the adequate treatment of fungal
infections like candidiasis. For certain antifungal agents, the determination of Minimal Fungicidal Concentration (MFC) and time
kill curve could be clinically more relevant than the determination of the Minimal Inhibitory Concentration (MIC). In this study,
MIC and MFC to fluconazole, amphotericin B and caspofungin against C. albicans isolates and the killing patterns obtained with
caspofungin and amphotericin B against susceptible and resistant strains to fluconazole were determined. The results of MICs
showed that all C. albicans isolates were highly susceptible to amphotericin B, but two isolates were fluconazole resistant. The...
Tipo: Journal article
Palavras-chave: Antifungal susceptibility; Candida albicans; Time kill curves.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100028
Molecular identification of methanogenic archaea from Surti buffaloes (bubalus bubalis), reveals
more hydrogenotrophic methanogens phylotypes
Provedor de dados: 58
Autores: Singh,K.M; Pandya,P.R; Parnerkar,S; Tripathi,A.K; Rank,D.N; Kothari,R.K; Joshi,C.G.
Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouses gases contributing
to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an
approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, a thorough knowledge of
the diversity of these microbes in breeds of buffaloes, as well as in response to geographical location and different diets, is
required. Therefore, molecular diversity of rumen methanogens in Surti buffaloes was investigated using 16S rRNA gene libraries
prepared from pooled rumen contents from three Surti buffaloes. A total of 171 clones were identified revealing 23 different
sequences...
Tipo: Journal article
Palavras-chave: Methanomicrobiales; Methanobacteriales; Surti buffaloes; Phylotypes; 16s DNA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100017
Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its
immunoglobulin and non-immunoglobulin fractions
Provedor de dados: 58
Autores: Oliveira,Inaiara R. de; Bessler,Heidi C.; Bao,Sonia N.; Lima,Renato de L.; Giugliano,Loreny G.
Enterotoxigenic Escherichia coli (ETEC) is the most common cause of diarrhea in children in developing countries and among
travelers to ETEC endemic areas. ETEC diarrhea is caused by colonization of the small intestine mediated by colonization factor
(CF) antigens, and subsequent elaboration of enterotoxins. Breast feeding has been related to protection against enteric infections.
The protective effect of human milk can be ascribed to its immunoglobulin content, specially secretory immunoglobulin A (sIgA),
and to non-immunoglobulin components such as free oligosaccharides, glycoproteins and glycolipids. In this study we
investigated the effect of whole human milk and its fractions immunoglobulin and non-immunoglobulin on the adherence of
ETEC strains...
Tipo: Journal article
Palavras-chave: Diarrhea; Adherence inhibition; Escherichia coli enterotoxigenic; Colonization factor antigen; Human milk.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100018
Detection of Legionella pneumophila in water and biofilm samples by culture and molecular methods
from man-made systems in São Paulo - Brazil
Provedor de dados: 58
Autores: Carvalho,Fábio R.S.; Foronda,Annette S.; Pellizari,Vivian H.
Legionella pneumophila is a pathogenic bacteria associated to aquatic habitat of natural and artificial environments. Clinical cases
of legionellosis have been reported in Brazil but there is a lack of information about the incidence and concentration of this
bacterium in environmental sources. Thus, the present study was designed to evaluate the occurrence of legionellae in São Paulo
city, Brazil, using different methods of detection and identification. Sixty-seven water and biofilm samples from natural reservoirs
and man-made systems were collected and analyzed for the presence of Legionella spp by culturing onto a selective medium,
coculture in axenic free-living amoebae and direct fluorescent antibody (DFA) assay. Results showed that freshwater of...
Tipo: Journal article
Palavras-chave: Legionella pneumophila; Free-living amoebae; Man-made systems.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400029
Viability of Staphylococcus xylosus isolated from artisanal sausages for application as starter cultures
in meat products
Provedor de dados: 58
Autores: Fiorentini,Ângela Maria; Sawitzki,Maristela Cortez; Bertol,Teresinha Marisa; Sant'Anna,Ernani S.
Viability of Staphylococcus xylosus isolated from artisanal sausages for application as starter cultures in meat products Viability
of Staphylococcus xylosus strains AD1 and U5isolated from natural fermented sausages was investigated as starter cultures in
fermented sausages produced in the South Region of Brazil. The study demonstrated that the Staphylococcus xylosus strains AD1
and U5showed significant growth during fermentation, stability over freeze-dried process, negative reaction for staphylococcal
enterotoxins and viability for using as a single-strain culture or associated with lactic acid bacteria for production of fermented
sausages.
Tipo: Journal article
Palavras-chave: Staphylococcus xylosus; Starter cultures; Sausages.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100022
Killer yeasts inhibit the growth of the phytopathogen Moniliophthora perniciosa, the causal agent of
Witches' Broom disease
Provedor de dados: 58
Autores: Cabral,Anderson de Souza; Carvalho,Patricia Maria Barroso de; Pinotti,Tatiana; Hagler,Allen Norton;
Mendonça-Hagler,Leda Cristina Santana; Macrae,Andrew.
Fruit and soil yeasts isolated from the Amazon, Atlantic Rainforests and an organic farm were screened for killer activity against
yeasts. Killer yeasts were then tested against the phytopathogen Moniliophthora perniciosa (syn. Crinipellis perniciosa) and a
Dipodascus capitatus strain and a Candida sp strain inhibited its growth.
Tipo: Journal article
Palavras-chave: Biocontrol; Killer yeast; Witches' Broom; Moniliophthora perniciosa; Crinipellis perniciosa.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100018
Nutritional requirement among Pseudomonas aeruginosa isolates recovered from respiratory clinical
specimens at a tertiary hospital from South of Brazil
Provedor de dados: 58
Autores: Perez,Leandro Reus Rodrigues; Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luís.
We screened 349 isolates of P. aeruginosa from cystic fibrosis (CF+) and non-cystic fibrosis (CF-) patients for the auxotrophy.
Fourteen (4.0%) were auxotrophic and among them only one was recovered from CF-patient showing that this characteristic is
strongly associated with cystic fibrosis. In total, a requirement for 5 different compounds (or combination) was verified and, of
these, methionine was the most common single amino acid required. Only one auxotrophic isolate was no able to produce biofilm
in vitro.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Auxotrophy; Cystic fibrosis; Biofilm.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300026
Comparative efficacies of Zataria multiflora essential oil and itraconazole against disseminated
Candida albicans infection in BALB/c mice
Provedor de dados: 58
Autores: Khosravi,A.R.; Shokri,H.; Tootian,Z.; Alizadeh,M.; Yahyaraeyat,R.
Disseminated candidiasis is a serious problem in public health that results from the invasion of Candida species, in particular
Candida albicans. The aim of this study was to compare the efficacies of Zataria multiflora essential oil and itraconazole in
clearing C. albicans from the visceral organs of BALB/c mice suffered from disseminated candidiasis. Zataria multiflora essential
oil was extracted using Clevenger-type apparatus and analyzed by gas chromatography mass spectrometry (GC-MS). For
clearance experiment, mice (20-25 g, N=8 per group) received essential oil at doses of 30, 48 and 64 mg/kg and itraconazole at
dose of 200 mg/kg intraperitoneally (IP) 2 days before and after intravenous inoculation of 0.5×10(6) C. albicans blastospores.
The treated...
Tipo: Journal article
Palavras-chave: Zataria multiflora essential oil; Candida albicans; Disseminated candidiasis; Carvacrol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300003
Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24
h fermentative cycles during the production of artisanal Brazilian cachaça
Provedor de dados: 58
Autores: Pataro,Carla; Guerra,Juliana B.; Gomes,Fátima C.O.; Neves,Maria J.; Pimentel,Patrícia F.; Rosa,Carlos A.
Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles
during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates,
70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over
40ºC. Lower temperatures (<40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera
japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and
accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol...
Tipo: Journal article
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Trehalose; Invertase; Fermentation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300003
Specific plant induced biofilm formation in Methylobacterium species
Provedor de dados: 58
Autores: Rossetto,Priscilla B; Dourado,Manuella N; Quecine,Maria C; Andreote,Fernando D; Araújo,Welington L;
Azevedo,João L; Pizzirani-Kleiner,Aline A.
Two endophytic strains of Methylobacterium spp. were used to evaluate biofilm formation on sugarcane roots and on inert
wooden sticks. Results show that biofilm formation is variable and that plant surface and possibly root exudates have a role in
Methylobacterium spp. host recognition, biofilm formation and successful colonization as endophytes.
Tipo: Journal article
Palavras-chave: Methylobacterium; Endophytes; Biofilm; Sugarcane.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300006
Draft genome sequence of blaVeb-1, blaoxa-10producing multi-drug resistant (MDR) Pseudomonas
aeruginosastrain VRFPA09 recovered from bloodstream infection
Provedor de dados: 58
Autores: Murugan,Nandagopal; Malathi,Jambulingam; Umashankar,Vetrivel; Madhavan,Hajib NarahariRao.
Pseudomonas aeruginosa (P. aeruginosa) bacteremia causes significant mortality rate due to emergence of multidrug resistant
(MDR) nosocomial infections. We report the draft genome sequence of P. aeruginosa strain VRFPA09, a human bloodstream
isolate, phenotypically proven as MDR strain. Whole genome sequencing on VRFPA09, deciphered betalactamase encoding
blaveb-1 and blaOXA-10genes and multiple drug resistance, virulence factor encoding genes.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Extended spectrum betalactamases; Next generation sequencing; Blood.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300639
A biotechnology perspective of fungal proteases
Provedor de dados: 58
Autores: Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de;
Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa
Junior,Adalberto; Magalhães,Pérola Oliveira.
Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are
essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial
processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in
industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce
production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters
such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development
of...
Tipo: Journal article
Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337
HIV-1 anti-retroviral drug effect on the C. albicans hyphal growth rate by a Bio-Cell Tracer system
Provedor de dados: 58
Autores: Melo,Nadja Rodrigues de; Vilela,Maria Marluce Santos; Jorge Junior,Jacks; Kamei,Katsuhiko; Miyaji,Makoto;
Fukushima,Kazutaka; Nishimura,Kazuko; Groeneveld,Philip; Kelly,Steven L.; Taguchi,Hideaki.
Declining incidence of oropharyngeal candidosis and opportunistic infections over recent years can be attributed to the use of
highly active anti-retroviral therapy (HAART). Infection with C. albicans generally involves adherence and colonization of
superficial tissues. During this process, budding yeasts are able to transform to hyphae and penetrate into the deep tissue. Using
the biocell tracer system, C. albicans hyphal growth was dynamically observed at the cellular level. Ritonavir was effective in the
inhibition of hyphal growth with growth rate of 0.8 mum/min. This study showed the in vitro effect of HIV anti-retroviral drug on
the growth rate of the C. albicans hyphae.
Tipo: Journal article
Palavras-chave: Candida; Hyphal; Protease inhibitor.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300006
Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and
sensitivity to sanitizers
Provedor de dados: 58
Autores: Souza,Evandro Leite de; Meira,Quênia Gramile Silva; Barbosa,Isabella de Medeiros; Athayde,Ana Júlia Alves Aguiar;
Conceição,Maria Lúcia da; Siqueira Júnior,José Pinto de.
This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated
from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7 ºC.
It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the
bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and
incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of
cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium...
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Food processing environment; Cross-contamination; Biofilm; Sanitizers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100010
Invasive Trichosporon cutaneum infection in an infant with wilms' tumor
Provedor de dados: 58
Autores: Carvalho,Ana Maria Rabelo de; Melo,Luciana Resende Bandeira de; Moraes,Vera Lúcia; Neves,Rejane Pereira.
Fungal infections are increasingly being reported in immuno-compromised patients. In this study we report a case of systemic
Trichosporon cutaneum infection in an infant with Wilms' tumor. This is the first time that an invasive infection for T. cutaneum
has been reported in a Wilms' tumor patient.
Tipo: Journal article
Palavras-chave: Fungal infections; Trichosporon cutaneum; An infant; Diagnosis; Wilms' tumor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100014
The neem [Azadirachta indica a: juss (meliaceae)] oil reduction in the in vitro production of
zearalenone by Fusarium graminearum
Provedor de dados: 58
Autores: Geraldo,Márcia Regina Ferreira; Costa,Christiane Luciana da; Arrotéia,Carla Cristina; Kemmelmeier,Carlos.
Zearalenone, a mycotoxin produced by fungi of the genus Fusarium, including F. graminearum, triggers reproduction disorders in
certain animals and hyperestrogen syndromes in humans. Current research investigates three concentrations of neem oil extract
(0.1, 0.25 and 0.5%) in reducing the production of zearalenone. Neem oil extract decreased zearalenone amount in the three
concentrations but highest inhibition (59.05%) occurred at 0.1%.
Tipo: Journal article
Palavras-chave: Zearalenone; Fusarium graminearum; Neem oil extract.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200036
Sensitivity of AMPLICOR MTB on direct detection of Mycobacterium tuberculosis in smear-negative
specimens from outpatients in Rio de Janeiro
Provedor de dados: 58
Autores: Anjos Filho,Luciano dos; Oelemann,Walter M.R.; Barreto,Clara E.N.; Kritski,Afranio Lineu; Fonseca,Leila de Souza.
We evaluated the performance of the AMPLICOR MTB assay for detection of M. tuberculosis in clinical specimens obtained
from 98 smear-negative tuberculous patients (43 with positive and 55 with negative culture results). The sensitivity of the
AMPLICOR MTB was 44.9% (44/98) and that of culture was 43.8% (43/98). No significant difference was observed between the
results obtained by AMPLICOR MTB and by culture. We conclude that amplification assays could be used for testing
smear-negative specimens because a rapid diagnosis will benefit those patients.
Tipo: Journal article
Palavras-chave: Tuberculosis; AMPLICOR MTB; Smear-negative specimens.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200013
Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.)
Provedor de dados: 58
Autores: Hayat,Sikandar; Christias,Christos.
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion
technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum
protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively. Osmotic stabilizer
sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from
strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and
F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on
carrots, beans and...
Tipo: Journal article
Palavras-chave: Protoplast; Fusion; Sclerotium rolfsii; Pathogenicity and osmotic stabilizer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100035
Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato
pomace
Provedor de dados: 58
Autores: Umsza-Guez,Marcelo A.; Díaz,Ana B.; Ory,Ignacio de; Blandino,Ana; Gomes,Eleni; Caro,Ildefonso.
In this work, tomato pomace, a waste abundantly available in the Mediterranean and other temperate climates agro-food
industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase,
exo-polygalacturonase (exo-PG), cellulase (CMCase) and α-amylase. The principal step of the process is the solid state
fermentation (SSF) of this residue by Aspergillus awamori. In several laboratory experiments, maximum xylanase and exo-PG
activities were measured during the first days of culture, reaching values around 100 and 80 IU/gds (international units of enzyme
activity per gram of dried solid), respectively. For CMCase and α-amylase production remained almost constant along
fermentation, with average values...
Tipo: Journal article
Palavras-chave: Solid state fermentation (SSF); Tomato pomace; Hydrolytic enzymes; Plate-type bioreactor; Xylanase
production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400046
Lectin activity in mycelial extracts of Fusarium species
Provedor de dados: 58
Autores: Bhari,Ranjeeta; Kaur,Bhawanpreet; Singh,Ram S.
ABSTRACT Lectins are non-immunogenic carbohydrate-recognizing proteins that bind to glycoproteins, glycolipids, or
polysaccharides with high affinity and exhibit remarkable ability to agglutinate erythrocytes and other cells. In the present study,
ten Fusarium species previously not explored for lectins were screened for the presence of lectin activity. Mycelial extracts of F.
fujikuroi, F. beomiformii, F. begoniae, F. nisikadoi, F. anthophilum, F. incarnatum, and F. tabacinum manifested agglutination of
rabbit erythrocytes. Neuraminidase treatment of rabbit erythrocytes increased lectin titers of F. nisikadoi and F. tabacinum
extracts, whereas the protease treatment resulted in a significant decline in agglutination by most of the lectins. Results of...
Tipo: Journal article
Palavras-chave: Fusarium; Lectin; Hemagglutination; Carbohydrate specificity; Culture age.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300775
Effect of benzene compounds from plants on the growth and hyphal morphology in Neurospora
crassa
Provedor de dados: 58
Autores: Neves,Fabrícia Mendonça; Kawano,Cristina Yoshiko; Said,Suraia.
The effects of the benzene compounds from plants, respectively cinnamic acid, coumaric acid, ferulic acid, caffeic acid, and
cinnamic aldehyde on growth and hyphal morphology of Neurospora crassa, were investigated. Cinnamic acid, ferulic acid and
cinnamic aldehyde inhibited colony growth, but produced no visible alterations on hyphae. Caffeic acid and coumaric acid did not
inhibit growth, but changed hyphal morphology. The results suggest that caffeic and coumaric acids probably affect polarity
maintenance (the continued deposition of wall material at the extending tip), while cinnamic aldehyde, ferulic and cinnamic acids
decrease growth rate, but did not change hyphal polarity. The actin cytoskeleton and the Spitzenkörper appeared diffuse and not
clearly...
Tipo: Journal article
Palavras-chave: Cinnamic acid; Ferulic acid; Cinnamic aldehyde.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200016
Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of
indigenous Pseudomonas sp. LDC-5 using molasses
Provedor de dados: 58
Autores: Saranya,V; Shenbagarathai,R.
Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria
as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived
plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper
attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from
agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ±
0.05-75.5%) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1%). Properties of the polymer produced from molasses and
sucrose were analyzed...
Tipo: Journal article
Palavras-chave: Polyhydroxyalkanoates; Molasses; Cost effective production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300032
Teat papillomatosis associated with bovine papillomavirus types 6, 7, 9, and 10 in dairy cattle from
Brazil
Provedor de dados: 58
Autores: Tozato,Claudia C.; Lunardi,Michele; Alfieri,Alice F.; Otonel,Rodrigo A.A.; Di Santis,Giovana W.; Alcântara,Brígida K.
de; Headley,Selwyn A.; Alfieri,Amauri A.
This study describes the clinical, histopathological, and virological characterization of teat papillomatosis from Brazilian dairy
cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10); one of these (BPV7) is being detected for
the first time in Brazilian cattle.
Tipo: Journal article
Palavras-chave: Papilloma; BPV; Genotyping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300039
Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
Provedor de dados: 58
Autores: Zepeda,Andrea B.; Figueroa,Carolina A.; Abdalla,Dulcineia S.P.; Maranhão,Andrea Q.; Ulloa,Patricio H.; Pessoa
Jr,Adalberto; Farías,Jorge G.
Pichia pastoris is methylotrophic yeast used as an efficient expression system for heterologous protein production. In order to
evaluate the effects of temperature (10 and 30 °C) and methanol (1 and 3% (v/v)) on genetically-modified Pichia pastoris,
different biomarkers were evaluated: Heat stress (HSF-1 and Hsp70), oxidative stress (OGG1 and TBARS) and antioxidant
(GLR). Three yeast cultures were performed: 3X = 3% methanol-10 °C, 4X = 3% methanol-30 °C, and 5X = 1% methanol-10°C.
The expression level of HIF-1α, HSF-1, HSP-70 and HSP-90 biomarkers were measured by Western blot and in situ
detection was performed by immunocytochemistry. Ours results show that at 3% methanol -30 °C there is an increase of
mitochondrial OGG1 (mtOGG1), Glutathione...
Tipo: Journal article
Palavras-chave: Oxidative stress; Recombinant yeast; Heat stress protein; DNA damage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200014
Effect of ingestion of soy yogurt on intestinal parameters of rats fed on a beef-based animal diet
Provedor de dados: 58
Autores: Bedani,Raquel; Pauly-Silveira,Nadiége Dourado; Cano,Veridiana Soares Pereira; Valentini,Sandro Roberto;
Valdez,Graciela Font de; Rossi,Elizeu Antonio.
The aim of this study was to investigate whether the ingestion of soy yogurt fermented with Enterococcus faecium CRL 183
would modify the intestinal count of enterococci, fecal pH and ammonia content in rats fed on a diet containing red meat. The rats
were placed in 4 groups: for 60 days, group I was given a standard casein-based rodent feed and groups II-IV, the beef-based feed.
From day 30, groups III-IV also received the following products: III) soy yogurt; IV) suspension of E. faecium CRL 183. At the
start and on days 30 and 60, feces were collected for the determination of pH, ammonia content, count of enterococci and
identification of their species. On day 60, rats were sacrificed and their colons also removed for count of enterococci and...
Tipo: Journal article
Palavras-chave: Soy yogurt; Enterococcus faecium CRL 183; Fecal pH; Fecal ammonia content; Rats.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300050
Comparative evaluation of pyrogens tests in pharmaceutical products
Provedor de dados: 58
Autores: Silveira,Rosimar L.; Andrade,Simone S.; Schmidt,Cleber A.; Casali,Renata G.; Dalmora,Sérgio L.
A comparison of methodologies for detection of pyrogens in pharmaceutical products was performed. The rabbit pyrogen test was
optimized and the dose-response curve was obtained for the 2nd International Standard for bacterial endotoxins, establishing
13.81 EU/mL/kg as the concentration of endotoxin necessary to induce a temperature rise of 0.5ºC. The 0.5ºC cut-off was shown
to give results that were more compatible with the pyrogenic doses for humans. The Limulus amoebocyte lysate test (LAL) was
standardized with gel-clot and chromogenic endpoints, and used for the comparative evaluation of pharmaceutical products
showing good agreement. The use of beta-glucan-reactive and non-reactive LAL reagents identified some products with
false-positive results. The...
Tipo: Journal article
Palavras-chave: Bacterial endotoxin; Limulus amoebocyte lysate; Pyrogens; Rabbit pyrogen test.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100007
Susceptibility to β-lactams and quinolones of Enterobacteriaceae isolated from urinary
tract infections in outpatients
Provedor de dados: 58
Autores: Marchisio,Martín; Porto,Ayelén; Joris,Romina; Rico,Marina; Baroni,María R.; Di Conza,José.
Abstract The antibiotic susceptibility profile was evaluated in 71 Enterobacteriaceae isolates obtained from outpatient urine
cultures in July 2010 from two health institutions in Santa Fe, Argentina. The highest rates of antibiotic resistance were observed
for ampicillin (AMP) (69%), trimethoprim/sulfamethoxazole (TMS) (33%), and ciprofloxacin (CIP) (25%). Meanwhile, 21% of
the isolates were resistant to three or more tested antibiotics families. Thirty integron-containing bacteria (42.3%) were detected,
and a strong association with TMS resistance was found. Third generation cephalosporin resistance was detected in only one
Escherichia coli isolate, and it was characterized as a blaCMY-2 carrier. No plasmid-mediated quinolone resistance (PMQR) was
found....
Tipo: Journal article
Palavras-chave: Urine tract infection outpatient β -lactam resistance fluoroquinolone resistance integrons.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401155
Enhanced alkaline cellulases production by the thermohalophilic Aspergillus terreus AUMC 10138
mutated by physical and chemical mutagens using corn stover as substrate
Provedor de dados: 58
Autores: Isaac,George Saad; Abu-Tahon,Medhat Ahmed.
Abstract A thermohalophilic fungus, Aspergillus terreus AUMC 10138, isolated from the Wadi El-Natrun soda lakes in northern
Egypt was exposed successively to gamma and UV-radiation (physical mutagens) and ethyl methan-sulfonate (EMS; chemical
mutagen) to enhance alkaline cellulase production under solid state fermentation (SSF) conditions. The effects of different carbon
sources, initial moisture, incubation temperature, initial pH, incubation period, inoculum levels and different concentrations of
NaCl on production of alkaline filter paper activity (FPase), carboxymethyl cellulase (CMCase) and β-glucosidase by
the wild-type and mutant strains of A. terreus were evaluated under SSF. The optimum conditions for maximum production of
FPase, CMCase...
Tipo: Journal article
Palavras-chave: Thermohalophilic fungus; Aspergillus terreus; Alkaline cellulase; Solid state fermentation; Corn stover.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401269
Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their
modulation by pH and carbon source
Provedor de dados: 58
Autores: Martínez-Trujillo,Aurora; Aranda,Juan S.; Gómez-Sánchez,Carlos; Trejo-Aguilar,Blanca; Aguilar-Osorio,Guillermo.
Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic
acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced
exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as
constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and
stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when
pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The
first enzyme to be...
Tipo: Journal article
Palavras-chave: Aspergillus flavipes; Pectinolytic enzymes; Constitutive enzymes; Inducible enzymes; Pectin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100006
Characterization of recombinant MSP5 Anaplasma marginale Havana isolate
Provedor de dados: 58
Autores: Corona,B.; Machado,H.; Rodríguez,M.; Martínez,S.
Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface
proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic
characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the
genus Anaplasma and in all isolates of A. marginale. The aim of the present work was to carry out the cloning, sequencing and
characterization of the recombinant MSP5 Anaplasma marginale Havana isolate. The sequence of the msp5 gene of Anaplasma
marginale Havana isolate with a size of 633 pb was determined (Acc. No. AY527217). This gene was cloned into pRSETB vector
and expressed...
Tipo: Journal article
Palavras-chave: Anaplasma marginale; Major surface proteins; Characterization; Havana isolate; HMSP5.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400032
Yields from blood cultures of patients with suspected paratyphoid fever A
Provedor de dados: 58
Autores: Wang,S.k.; Chu,C.J.; Shan,D.S.; Sun,P.S.; Wu,Q.
The yield and speed of detection of Salmonella enterica serotype Paratyphi A from the blood of patients with suspected
paratyphoid fever A in 13 500 paired aerobic and anaerobic bottles (AEB, ANB) that were each filled with 5 ml of blood by the
BacT/ALERT 3D system were compared, and the blood bacterial counts of 1 000 probable patients were estimated by pour plate
method. A total of 4 060 isolates were recovered, of these, 3 149 were recovered from both AEB and ANB, 461 from the AEB
only, and 450 from the ANB only. The estimating median bacterial count in blood from 400 patients was 0.5 CFU/ml. The
research findings demonstrate that the blood volume drawn is an important factor determining the yields from blood cultures.
Growth of significantly more...
Tipo: Journal article
Palavras-chave: Paratyphoid fever; Salmonella enterica serotype Paratyphi A; Blood Culture; Bacterial counts in blood.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200003
Prevalence of resistance to aminoglycosides and fluoroquinolones among Pseudomonas aeruginosa
strains in a University Hospital in Northeastern Poland
Provedor de dados: 58
Autores: Michalska,Anna Diana; Sacha,Pawel Tomasz; Ojdana,Dominika; Wieczorek,Anna; Tryniszewska,Elzbieta.
The present study was conducted to investigate the prevalence of genes encoding resistance to aminoglycosides and
fluoroquinolones among twenty-five Pseudomonas aeruginosa isolated between 2002 and 2009. In PCR, following genes were
detected: ant(2")-Ia in 9 (36.0%), aac(6')-Ib in 7 (28.0%), qnrB in 5 (20.0%), aph(3")-Ib in 2
(8.0%) of isolates.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Plasmid-mediated resistance to aminoglycosides and fluoroquinolones;
Aminoglycoside-modifying enzymes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400041
Bioconversion of R-(+)-limonene to perillic acid by the yeast Yarrowia lipolytica
Provedor de dados: 58
Autores: Ferrara,Maria Antonieta; Almeida,Débora S.; Siani,Antonio C.; Lucchetti,Leonardo; Lacerda,Paulo S.B.; Freitas,André;
Tappin,Marcelo R.R.; Bon,Elba P.S.
Perillyl derivatives are increasingly important due to their flavouring and antimicrobial properties as well as their potential as
anticancer agents. These terpenoid species, which are present in limited amounts in plants, may be obtained via bioconversion of
selected monoterpene hydrocarbons. In this study, seventeen yeast strains were screened for their ability to oxidize the exocyclic
methyl group in the p-menthene moiety of limonene into perillic acid. Of the yeast tested, the highest efficiency was observed for
Yarrowia lipolytica ATCC 18942. The conversion of R (+)-limonene by Y. lipolytica was evaluated by varying the pH (3 to 8)
and the temperature (25 to 30 ºC) in a reaction medium containing 0.5% v/v limonene and 10 gµL of stationary phase cells...
Tipo: Journal article
Palavras-chave: Yarrowia lipolytica; Bioconversion; Limonene; Perillic acid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400008
Epidemiology of oral HPV in the oral mucosa in women without signs of oral disease from Yucatan,
Mexico
Provedor de dados: 58
Autores: Gonzalez-Losa,María del Refugio; Barrera,Ernesto Soria; Herrera-Pech,Verónica; Conde-Ferráez,Laura;
Puerto-Solís,Marylin; Ayora-Talavera,Guadalupe.
High-risk human papillomaviruses (HR-HPV) are considered necessary for the development of cervical cancer. Furthermore,
there is no doubt that some types of oral squamous cell carcinoma are associated with HR-HPV. The epidemiology of oral HPV
infections in healthy subjects remains unclear due to a lack of knowledge. The objective of this study was to investigate the
epidemiology of human papillomavirus infections of the oral mucosa without pathology. A cross-sectional study was performed;
samples from 390 women seeking prenatal care, Pap smears, family planning or gynecological diseases were studied. Oral cells
were collected by direct swab sampling. Information regarding sociodemographic status, sexual behavior, infectious diseases,
contraceptive history...
Tipo: Journal article
Palavras-chave: Papillomavirus; Oral mucosa; HPV13; Mexico.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100301
Fusarium lateritium (NEES) as an agent of fungemia in a patient infected with the human
immunodeficiency virus (HIV)
Provedor de dados: 58
Autores: Cambuim,Idalina Inês Fonsêca Nogueira; Neves,Rejane Pereira; Queiroz,Lusinete Aciole de; Magalhães,Oliane Maria
Correia; Lima,Débora Maria Massa.
Emerging fungal pathogens are associated with significant morbidity and mortality in the immunocompromised host. The
association of fungi from the Fusarium genus with human infection in uncommon. The objective of this paper is to report the first
case of fungaemia caused by Fusarium lateritium in a 42-year-old HIV-infected patient.
Tipo: Journal article
Palavras-chave: Fungaemia; Fusarium lateritium; HIV-infection; Immunocompromissed.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200018
Hemibeltrania urbanodendrii sp. nov. and Pseudobeltrania angamosensis: new fungal records from
the brazilian tropical seasonal semi-deciduous montane forest
Provedor de dados: 58
Autores: Fernandes,Ronaldo de Castro; Lustosa,Denise Castro; Barreto,Robert Weingart; Bezerra,José Luiz.
The new species Hemibeltrania urbanodendrii, associated to leaf-spots on Urbanodendron verrucosum (Lauracea) and
Pseudobeltrania angamosensis, associated with leaf-spots on Virola gardneri (Myristicaceae), are recorded for the first time in
Brazil. They represent additions to the mycobiota of the Tropical Seasonal Semi-Deciduous Montane Forest in Viçosa (Minas
Gerais, Brazil), a highly threatened ecosystem.
Tipo: Journal article
Palavras-chave: Beltrania-like fungi; Urbanodendron verrucosum; Virola gardneri; Biodiversity; Fungal diversity; Mycobiota.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400031
Diversity of leaf endophytic fungi in mangrove plants of Northeast Brazil
Provedor de dados: 58
Autores: Costa,Isabella P. M. Wanderley; Maia,Leonor Costa; Cavalcanti,Maria Auxiliadora.
With the aim of increasing the knowledge about endophytic fungi, a group of microorganisms with high biotechnological potential
and a valuable source of useful metabolites, a survey in leaves of mangrove plants (Avicennia schaueriana, Laguncularia
racemosa, and Rhizophora mangle) was performed at the Itamaracá Island, PE, Brazil. Leaves were collected, during two seasons,
dry and rainy, superficially sterilized and fragments maintained in Petri dishes with Potato dextrose agar (PDA) at 28º ± 2º C until
isolation of the fungi. Fourty taxa were isolated: 25 species representing 19 genera and 15 morphotypes determined as Mycelia
sterilia. Leaves of L. racemosa hosted the highest number of colony forming units (CFU) and taxa. Guignardia sp. and
Colletotrichum...
Tipo: Journal article
Palavras-chave: Aviccenia; Conidial fungi; Laguncularia; Rhizophora.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300044
Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP
Provedor de dados: 58
Autores: Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.;
Chanda,S.V.
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In
the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of
Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully
discriminate among the ‘A’, ‘B’ and ‘G’ group A.
flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could
be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed
within a district...
Tipo: Journal article
Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673
Quality control for microbiological culture media: Is it enough to follow the NCCLS M22-A2
procedures?
Provedor de dados: 58
Autores: Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza;
Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas.
Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of
organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect
medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The
detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some
fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised.
Tipo: Journal article
Palavras-chave: Quality control; Culture medium; NCCLS.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003
Dissolving mechanism of strain P17 on insoluble phosphorus of yellow-brown soil
Provedor de dados: 58
Autores: Chuan-qing,Zhong; Guang-xiang,Cao; Wei-yi,Huang; Xing-she,Luan; Yi-fei,Yang.
Strain P17 was a bacterial strain identified as Bacillus megaterium isolated from ground accumulating phosphate rock powder.
The fermentation broth of strain P17 and the yellow-brown soil from Nanjing Agricultural University garden were collected to
conduct this study. The simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into
yellow-brown soil was performed in pots, while available P and total P of soil were extremely positive correlative with those of
groundwater. Then the dissolving effect of strain P17 on insoluble P of yellow-brown soil was studied. Results showed that
Bacillus megaterium strain P17 had notable solubilizing effect on insoluble phosphates formed when too much water-soluble
phosphorous fertilizer...
Tipo: Journal article
Palavras-chave: Calcium superphosphate; P-solubilizing microbes; Acid phosphatase; Alkaline phosphatase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300025
Aeration effect on Spirulina platensis growth and γ-linolenic acid production
Provedor de dados: 58
Autores: Ronda,Srinivasa Reddy; Bokka,Chandra Sekhar; Ketineni,Chandrika; Rijal,Binod; Allu,Prasada Rao.
The influence of aeration on algal growth and gamma-linolenic acid (GLA) production in a bubble column photobioreactor was
investigated. Studies were performed in a 20-L reactor at different aeration rates (0.2-2.5 vvm). Static, continuous, and periodic
operation of air resulted in 41.9%, 88.4%, and 108% air saturation of dissolved oxygen, for which the corresponding values of
GLA were 2.3, 6.5, and 7.5 mg·g-1 dry cell weight, respectively. An increase in the aeration rate from 0.2 to 2.5 vvm enhanced
both the specific growth rate and GLA content under periodic sparging in the bicarbonate medium. With a 6-fold increase in the
aeration rate, the GLA content of the alga increased by 69.64% (5.6-9.5 mg· g-1 dry cell weight). In addition, the total fatty acid...
Tipo: Journal article
Palavras-chave: Spirulina platensis; Aeration; Photobioreactor; Gamma-linolenic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100002
Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station
Provedor de dados: 58
Autores: Mariano,Adriano Pinto; Kataoka,Ana Paula de Arruda Geraldes; Angelis,Dejanira de Franceschi de; Bonotto,Daniel
Marcos.
The purpose of the present study was to investigate possible methods to enhance the rate of aerobic biodegradation of
hydrocarbons (ex-situ treatments). In this work, the bioremediation processes were applied to a sandy soil with a high level of
contamination originated from the leakage of a diesel oil underground storage tank at a petrol station. Laboratory scale
experiments (Bartha biometer flasks) were used to evaluate the biodegradation of the diesel oil. Enhancement of biodegradation
was carried out through biostimulation (addition of nitrogen and phosphorus solutions or Tween 80 surfactant) and
bioaugmentation (bacterial consortium isolated from a landfarming system). To investigate interactions between optimizing
factors, and to find the right...
Tipo: Journal article
Palavras-chave: Bioremediation; Soil; Diesel Oil; Petrol Station.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200030
Microbial modeling of thermal resistance of Alicyclobacillus acidoterrestris CRA7152 spores in
concentrated orange juice with nisin addition
Provedor de dados: 58
Autores: Peña,Wilmer Edgard Luera; Massaguer,Pilar Rodriguez de; Teixeira,Luciano Quintão.
The nisin effect on thermal death of Alicyclobacillus acidoterrestris CRA 7152 spores in concentrated orange juice (64ºBrix) was
studied. Concentrations of 0, 50, 75 and 100 IU of nisin/ml juice, at temperatures of 92, 95, 98 and 102ºC were evaluated. The
quadratic polynomial model was used to analyze the effects of the factors and their interaction. Verification of surviving spores
was carried out through plating in K medium (pH 3.7). The results showed that the D values without nisin addition were 25.5,
12.9, 6.1 and 2.3 min for 92, 95, 98 and 102ºC respectively. With addition of nisin into the juice there was a drop of heat
resistance as the concentration was increased at a same temperature. With 30, 50, 75, 100 and 150 IU/ml at 95ºC, the D values
were...
Tipo: Journal article
Palavras-chave: Alicyclobacillus acidoterrestris; Orange juice concentrate; Modeling.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300024
Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD
Provedor de dados: 58
Autores: Colauto,Nelson Barros; Dias,Eustáquio Souza; Gimenes,Marcos Aparecido; Eira,Augusto Ferreira da.
The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little
genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to
characterize this fungus.
Tipo: Journal article
Palavras-chave: Agaricus blazei; Basidiomycete; Genetic diversity; Mushroom; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200006
Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with
aggressive periodontitis
Provedor de dados: 58
Autores: Rosalem Junior,Wilson; Souza,Rodrigo Carvalho de; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has
also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease.
The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in
patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP
subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment
level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects....
Tipo: Journal article
Palavras-chave: Generalized aggressive periodontitis; PCR; Actinobacillus actinomycetemcomitans; Saliva; Leukotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
Antifungal activity against Cryptococcus neoformans strains and genotoxicity assessment in human
leukocyte cells of Euphorbia tirucalli L.
Provedor de dados: 58
Autores: Oliveira,Luís Flávio Souza de; Fuentefria,Alexandre Meneghello; Klein,Fernanda da Silva; Machado,Michel Mansur.
In the last times, focus on plant research has increased all over the world. Euphorbia tirucalli L., a plant known popularly as
Aveloz, and originally used in Africa, has been drawing attention for its use in the United States and Latin America, both for use
as an ornamental plant and as a medicinal plant. E. tirucalli L. is a member of the family Euphorbiaceae and contains many
diterpenoids and triterpenoids, in particular phorbol esters, apparently the main constituent of this plant, which are assumed to be
responsible for their activities in vivo and in vitro. The in vitro antifungal activities of Euphorbia tirucalli (L.) against
opportunistic yeasts were studied using microbroth dilution assay. The results showed that aqueous extract and latex...
Tipo: Journal article
Palavras-chave: Euphorbia tirucalli L.; Aveloz; Antifungal; Cryptococcus neoformans.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400027
Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp.,
Scleroderma sp. and Suillus sp.
Provedor de dados: 58
Autores: Silva,R.F.; Lupatini,M.; Trindade,L.; Antoniolli,Z.I.; Steffen,R.B.; Andreazza,R.
Environments contaminated with heavy metals negatively impact the living organisms. Ectomy-corrhizal fungi have shown
important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates
Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25
mmol L-1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L-1 in the liquid medium. Copper was amended as copper
sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The
fungal isolates were incubated...
Tipo: Journal article
Palavras-chave: Ectomycorrhizal fungus; Copper-resistance; Pisolithus microcarpus; Pisolithus sp.; Scleroderma sp.; Suillus sp.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200042
Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or
hematopoietic stem cell transplantation patients
Provedor de dados: 58
Autores: Dieamant,Débora de Campos; Bonon,Sandra Helena Alves; Prates,Liliane Cury; Belangelo,Vera Maria Santoro;
Pontes,Erika R.; Costa,Sandra Cecília Botelho.
A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or
hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different
gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the
monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients
studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different
HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone
marrow transplantation...
Tipo: Journal article
Palavras-chave: Cytomegalovirus; Pediatric transplantation; GB genotypes; PCR; Antigenemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100010
Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis
on strains of Staphylococcus aureus
Provedor de dados: 58
Autores: Pamplona-Zomenhan,Lucila Coelho; Pamplona,Beatriz Coelho; Silva,Cely Barreto da; Marcucci,Maria Cristina;
Mimica,Lycia Mara Jenné.
Staphylococcus aureus (S. aureus) is one of the most frequent causes of hospital acquired infections. With the increase in multiple
drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims of this study were: to
determine the in vitro antimicrobial activity of an ethanol extract of propolis; to define the MIC50 and MIC90 (Minimal Inhibitory
Concentration - MIC) against 210 strains of S. aureus; to characterize a crude sample of propolis and the respective ethanol extract
as to the presence of predetermined chemical markers. The agar dilution method was used to define the MIC and the high
performance liquid chromatography (HPLC) method was used to characterize the samples of propolis. MIC results ranged...
Tipo: Journal article
Palavras-chave: Propolis; Staphylococcus aureus; Antimicrobial Activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400002
Enhancement of soil DNA extraction by the use of a hand held mixer
Provedor de dados: 58
Autores: Costa,Jefferson Luis da Silva; Oliveira,Virgínia Carla de.
The efficiency of soil DNA extraction using a bead beater homogenizer (Biospec Products - Germany) was compared to that
obtained with a hand held mixer (Moulinex - Brazil). The hand held mixer costs 100 times less and extracted seven times more
crude DNA than the bead beater.
Tipo: Journal article
Palavras-chave: Molecular tools; DNA extraction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400004
Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans
cultivated in brazilian cerrado soils
Provedor de dados: 58
Autores: Oliveira,Isnia Aparecida de; Vasconcellos,Maria José; Seldin,Lucy; Paiva,Edilson; Vargas,Milton Alexandre; Sá,Nadja
Maria Horta de.
Efficient bean nodulating Rhizobium strains, isolated from different Brazilian cerrado soils, were characterized by RAPD. This
study showed great genetic heterogeneity among R. tropici and R. leguminosarum bv. phaseoli strains and allowed the
constitution of genetic clusters, besides indicating the most suitable primers for this characterization. The groups of genetically
distinct strains can be used in competitiveness studies to select appropriate Rhizobium strains for bean inoculation in cerrado soils.
Tipo: Journal article
Palavras-chave: Rhizobium tropici; Rhizobium leguminosarum bv. phaseoli; Bean plants; Cerrado soils.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100010
Isolation and characterization of a Pichia anomala strain: a promising candidate for bioethanol
production
Provedor de dados: 58
Autores: Tao,Nengguo; Gao,Yumei; Liu,Yuejin.
A yeast strain designated as Y-1 was isolated and characterized from wine yeast ("Jiuqu"). Based on the morphological and
biochemical results, along with the rDNA internal transcribed spacer region (ITS), Y-1 was identified to be a Pichia anomala
strain. Y-1 is an ethanol-tolerant strain, enduring ethanol concentrations of up to 14 %. Y-1 growth medium conditions were
optimized, results showing good growth in medium with pH ranges from 3.5-6.5, temperature ranges from 25-30 °C, and
inoculums range of 8 %-12 %, while optimum growth conditions were reached at a temperature of 30 °C, pH 5.0, and inoculums
of 10 %. Furthermore, when the alkaline hydrolyzed Shatian pummelo peel solutions were inoculated with 10 % Y-1 and
fermented at 30 °C for 6 d, 4.7 % pure...
Tipo: Journal article
Palavras-chave: Bioethanol; Gas chromatography analysis; Jiuqu; Pichia anomala.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200031
Iron bioaccumulation in mycelium of Pleurotus ostreatus
Provedor de dados: 58
Autores: Almeida,Sandra M.; Umeo,Suzana H.; Marcante,Rafael C.; Yokota,Meire E.; Valle,Juliana S.; Dragunski,Douglas C.;
Colauto,Nelson B.; Linde,Giani A.
Pleurotus ostreatus is able to bioaccumulate several metals in its cell structures; however, there are no reports on its capacity to
bioaccumulate iron. The objective of this study was to evaluate cultivation variables to increase iron bioaccumulation in P.
ostreatusmycelium. A full factorial design and a central composite design were utilized to evaluate the effect of the following
variables: nitrogen and carbon sources, pH and iron concentration in the solid culture medium to produce iron bioaccumulated in
mycelial biomass. The maximum production of P. ostreatus mycelial biomass was obtained with yeast extract at 2.96 g of nitrogen
L−1 and glucose at 28.45 g L−1. The most important variable to bioaccumulation was the iron...
Tipo: Journal article
Palavras-chave: Iron sulfate; Functional food; Hemoglobin; Anemia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100195
Mycological contamination in dental unit waterlines in Istanbul, Turkey
Provedor de dados: 58
Autores: Kadaifciler,Duygu Göksay; Ökten,Suzan; Sen,Burhan.
Studies on dental units (DUs) are conducted either for the prevention or the reduction of the density of bacterial contamination in
dental unit waterlines (DUWLs). However, the existence of fungi in the these systems requires more attention. During dental
treatment, direct contact with water contaminated with fungi such as Candida, Aspergillus, or inhalation of aerosols from
high-speed drill may cause various respiratory infections, such as asthma, allergies, and wounds on mucose membranes, especially
on immunocompromised patients and dentists. The aims of this study are to investigate the number and colonization of fungi in
DUWLs in the city of Istanbul, Turkey. Water samples were collected from air-water syringes, high-speed drills, and inlet waters
from...
Tipo: Journal article
Palavras-chave: Dental unit waterlines; Dental equipments; Filamentous fungi; Mycological contamination; Yeast.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300049
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State,
Brazil
Provedor de dados: 58
Autores: Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide
Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda.
With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts)
and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August
2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar
Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive
tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of
two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,...
Tipo: Journal article
Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017
The influence of ripening period length and season on the microbiological parameters of a traditional
Brazilian cheese
Provedor de dados: 58
Autores: Cardoso,Valéria M.; Dias,Ricardo S.; Soares,Barbara M.; Clementino,Letícia A.; Araújo,Cristiano P.; Rosa,Carlos A.
The ripening process of Serro Minas cheese, one of the most popular cheeses produced with raw milk in Brazil, was studied over
the course of 60 days of ripening during dry and rainy seasons. Brazilian legislation prohibits the production of cheese from raw
milk unless it was submitted to a maturation period greater than 60 days. However Minas Serro cheese is sold within a few days of
ripening. A total of 100 samples of Serro cheese were obtained from five farms; 50 samples were collected during the dry season
(winter in Brazil) and 50 samples were collected during the rainy season (summer in Brazil). From each farm, ten cheeses were
collected during each season after two days of ripening. Our results showed high levels of total and fecal coliforms at the...
Tipo: Journal article
Palavras-chave: Traditional Minas cheese; Ripening; Total and fecal coliforms; Coagulase-positive staphylococci;
Staphylococcal enterotoxins.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300012
New antimicrobial combinations: substituted chalcones- oxacillin against methicillin resistant
Staphylococcus aureus
Provedor de dados: 58
Autores: Talia,Juan Manuel; Debattista,Nora Beatriz; Pappano,Nora Beatriz.
Staphylococcus aureus, the most virulent Staphylococcus species, is also the prevalent pathogen isolated from hospitalized
patients and the second most common from patients in outpatient settings. In general, bacteria have the genetic ability to transmit
and acquire resistance to drugs, which are utilized as therapeutic agents. Related studies of antimicrobial activity indicate that
crude extracts containing flavonoids, triterpenes and steroids have showed significative activity against several Staphylococcus
aureus strains. Combination effects between flavonoids and antibiotics also have been reported. The aim of the present work was
to investigate in vitro synergism between several chalcones substituted in combination with oxacillin, an antibiotic used...
Tipo: Journal article
Palavras-chave: Substituted chalcones; Oxacillin; Combinations; Antimicrobial; S. aureus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200010
Vaginal lactobacilli as potential probiotics against Candida spp
Provedor de dados: 58
Autores: Gil,Natalia F.; Martinez,Rafael C.R.; Gomes,Bruna C.; Nomizo,Auro; Martinis,Elaine C. P. De.
Urogenital infections affect millions of people every year worldwide. The treatment of these diseases usually requires the use of
antimicrobial agents, and more recently, the use of probiotic lactic acid bacteria (LAB) cultures for the management of vaginal
infections has been extensively studied. In this work, 11 vaginal lactobacilli isolates, previously obtained from healthy patients,
were studied to screen microorganisms with probiotic properties against Candida spp. The LAB were tested for their ability of
auto-aggregation, coaggregation with C. albicans, C. glabrata, C. krusei, and C. tropicalis, adhesion to Caco-2 epithelial cells and
production of lactic acid and hydrogen peroxide (H2O2). All lactobacilli isolates tested were able to auto-aggregate...
Tipo: Journal article
Palavras-chave: Lactobacillus spp.; Probiotic; Candida spp.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100002
A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Provedor de dados: 58
Autores: Qiu,Shanlian; Chen,Jichen; Lin,Si; Lin,Xinjian.
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed
important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low
background, including the Bassam et al. methods, the manufacturer method and our improved method.
Tipo: Journal article
Palavras-chave: Backed gel; DNA; Silver staining.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029
Increase of stress resistance in Lactococcus lactis via a novel food-grade vector expressing a shsp gene
from Streptococcus thermophilus
Provedor de dados: 58
Autores: Tian,Hongtao; Tan,Jianxin; Zhang,Lifang; Gu,Xinxi; Xu,Wentao; Guo,Xinghua; Luo,Yunbo.
The effects of the expression of a small heat shock protein (shsp) gene from Streptococcus thermophilus on stress resistance in
Lactococcus lactis under different environmental stresses were investigated in this study. pMG36e-shsp, an expression vector, was
first constructed by inserting a shsp open reading frame (ORF) cloned from S. thermophilus strain St-QC into pMG36e. Then, a
food-grade expression vector, pMG-shsp, was generated by deleting the erythromycin resistance gene from pMG36e-shsp. The
transformation rate of pMG-shsp was comparable to that of pMG36e-shsp when each of these two vectors was introduced into L.
lactis. These results demonstrated that the shsp ORF could successfully used as a food-grade selection marker in both pMG-shsp
and...
Tipo: Journal article
Palavras-chave: Shsp gene; Streptococcus thermophilus; Lactococcus lactis; Food-grade vector; Stress resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300043
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans
191
Provedor de dados: 58
Autores: Fleuri,Luciana F.; Kawaguti,Haroldo Y.; Sato,Hélia H.
This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans
strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L
fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and
using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose
CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form
protoplasts.
Tipo: Journal article
Palavras-chave: Chitinase; Cellulosimicrobium cellulans; Fungal lysis; Protoplasts.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026
Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility
patterns
Provedor de dados: 58
Autores: Castellano-Filho,Didier Silveira; Silva,Vânia Lúcia da; Nascimento,Thiago César; Vieira,Marcel de Toledo;
Diniz,Cláudio Galuppo.
Group B Streptococcus (GBS) is still not routinely screened during pregnancy in Brazil, being prophylaxis and empirical
treatment based on identification of risk groups. This study aimed to investigate GBS prevalence in Brazilian pregnant women by
culture or polymerase chain reaction (PCR) associated to the enrichment culture, and to determine the antimicrobial susceptibility
patterns of isolated bacteria, so as to support public health policies and empirical prophylaxis. After an epidemiological survey,
vaginal and anorectal specimens were collected from 221 consenting laboring women. Each sample was submitted to enrichment
culture and sheep blood agar was used to isolate suggestive GBS. Alternatively, specific PCR was performed from enrichment
cultures....
Tipo: Journal article
Palavras-chave: Perinatal disease; Group B streptococci; Antimicrobial drug susceptibility.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400024
Invasive infection due to Saprochaete capitata in a young patient with hematological malignancies
Provedor de dados: 58
Autores: Parahym,Ana Maria Rabelo de Carvalho; Rolim Neto,Pedro José; Silva,Carolina Maria da; Domingos,Igor de Farias;
Gonçalves,Sarah Santos; Leite,Edinalva Pereira; Morais,Vera Lúcia Lins de; Macêdo,Danielle Patrícia Cerqueira; Lima
Neto,Reginaldo Gonçalves de; Neves,Rejane Pereira.
We report a case of invasive infection due to Saprochaete capitata in a patient with hematological malignancies after
chemotherapy treatment and empiric antifungal therapy with caspofungin. Although severely immunocompromised the patient
survived been treated with amphotericin B lipid complex associated with voriconazole.
Tipo: Journal article
Palavras-chave: Invasive infection; Saprochaete capitata; Geotrichum capitatum; Hematological malignancies; Antifungal
susceptibility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200527
Production of bioethanol using agricultural waste: banana pseudo stem
Provedor de dados: 58
Autores: Ingale,Snehal; Joshi,Sanket J.; Gupte,Akshaya.
India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available
agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source
for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains
Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used
under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars.
The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to
produce...
Tipo: Journal article
Palavras-chave: Bioethanol; Banana pseudostem; Aspergillus ellipticus; Aspergillus fumigatus; Saccharomyces cerevisiae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300018
Evaluation of antimicrobial activity of extracts of Tibouchina candolleana (melastomataceae), isolated
compounds and semi-synthetic derivatives against endodontic bacteria
Provedor de dados: 58
Autores: Santos,Fernanda M. dos; Souza,Maria Gorete de; Crotti,Antônio E. Miller; Martins,Carlos H. G.; Ambrósio,Sérgio R.;
Veneziani,Rodrigo C. S.; Silva,Márcio L. Andrade e; Cunha,Wilson R.
This work describes the phytochemical study of the extracts from aerial parts of Tibouchina candolleana as well as the evaluation
of the antimicrobial activity of extracts, isolated compounds, and semi-synthetic derivatives of ursolic acid against endodontic
bacteria. HRGC analysis of the n-hexane extract of T. candolleana allowed identification of b-amyrin, a-amyrin, and b-sitosterol
as major constituents. The triterpenes ursolic acid and oleanolic acid were isolated from the methylene chloride extract and
identified. In addition, the flavonoids luteolin and genistein were isolated from the ethanol extract and identified. The
antimicrobial activity was investigated via determination of the minimum inhibitory concentration (MIC) using the broth...
Tipo: Journal article
Palavras-chave: Tibouchina candolleana; Ursolic acid; Antimicrobial activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200045
Habituation of enterotoxigenic Staphylococcus aureus to Origanum vulgare L. essential oil does not
induce direct-tolerance and cross-tolerance to salts and organic acids
Provedor de dados: 58
Autores: Tavares,Adassa Gama; Monte,Daniel Farias Marinho do; Albuquerque,Allan dos Reis; Sampaio,Fábio Correia;
Magnani,Marciane; Siqueira Júnior,José Pinto de; Souza,Evandro Leite de.
Enterotoxigenic Staphylococcus aureus strains that were isolated from foods were investigated for their ability to develop
direct-tolerance and cross-tolerance to sodium chloride (NaCl), potassium chloride (KCl), lactic acid (LA) and acetic acid (AA)
after habituation in sublethal amounts (1/2 of the minimum inhibitory concentration - 1/2 MIC and 1/4 of the minimum inhibitory
concentration - 1/4 MIC) of Origanum vulgare L. essential oil (OVEO). The habituation of S. aureus to 1/2 MIC and 1/4 MIC of
OVEO did not induce direct-tolerance or cross-tolerance in the tested strains, as assessed by modulation of MIC values.
Otherwise, exposing the strains to OVEO at sublethal concentrations maintained or increased the sensitivity of the cells to the
tested...
Tipo: Journal article
Palavras-chave: Staphylococcus; Adaptation; Essential oil; Oregano.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300835
Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12
Provedor de dados: 58
Autores: Farrokh,Parisa; Yakhchali,Bagher; Asghar Karkhane,Ali.
A mesophilic Enterobacter sp. Bn12 producing an alkaline thermostable lipase was isolated from soil in Tehran, Iran. The lipase
gene (ELBn12) was identified from a genomic library. Sequence analysis of the DNA fragment revealed an open reading frame of
879 bp encoding a lipase with a molecular mass of 31.3 kDa. The deduced amino acid sequence showed 96% identity with a
lipase of Enterobacter sp. Ag1 and the identity of their DNA sequences was 88.9%. ELBn12 belongs to the lipase subfamily I.1
and its catalytic triad consists of Ser82, Asp237 and His259. The lipase was expressed in Escherichia coli (BL21) pLysS and
partially purified by anion exchange chromatography. The maximum activity of ELBn12 was obtained at temperature of 60 °C
and pH 8.0 towards...
Tipo: Journal article
Palavras-chave: Enterobacter; Thermostable lipase; Alkaline; Organic solvent.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200042
Levels of infection and colonization of some oral bacteria after use of naf, chlorhexidine and a
combined chlorhexidine with naf mouthrinses
Provedor de dados: 58
Autores: Lima,Kenio Costa; Neves,Aline A.; Beyruth,Janine B.; Magalhães,Fernando A.C.; Uzeda,Milton de.
This study was carried out to evaluate levels of infection of bacteria involved in caries and oral streptococci and their colonization
after use of mouthrinses with NaF, chlorhexidine and chlorhexidine plus NaF. Combination of chlorhexidine and fluoride was the
only agent that reduced levels of Streptococcus mutans (group) for 30 days.
Tipo: Journal article
Palavras-chave: Chlorhexidine; Sodium fluoride; Cariogenic bacteria; Oral streptococci.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200018
Identification of lactic acid bacteria associated with traditional cachaça fermentations
Provedor de dados: 58
Autores: Gomes,Fatima C. O.; Silva,Carol L. C.; Vianna,Cristina R.; Lacerda,Inayara C. A.; Borelli,Beatriz M.; Nunes,Álvaro C.;
Franco,Gloria R.; Mourão,Marina M.; Rosa,Carlos A.
During the production of traditional cachaça (alembic´s cachaça), contamination of the fermented must is one of the
factors leading to economic losses in the beverage manufacturing industry. The diversity of bacterial populations and the role of
these microorganisms during the cachaça production process are still poorly understood in Brazil. In our work, the fermentation
process was followed in two distilleries located in the state of Minas Gerais. The objective of this work was to identify the
populations of lactic acid bacteria present during cachaça fermentation using physiological and molecular methods. Lactic acid
bacteria were isolated in high frequencies during all of the fermentative processes, and Lactobacillus plantarum and L. casei...
Tipo: Journal article
Palavras-chave: Lactic acid bacteria; Fermentation; Cachaça.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200031
A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri
Provedor de dados: 58
Autores: Dantas,Giordanni C.; Martins,Paula M.M.; Martins,Daniela A.B.; Gomes,Eleni; Ferreira,Henrique.
Abstract Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most
devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is
usually achieved by the elimination of affected plants along with that of asymptomatic neighbors. An in depth understanding of
the pathogen is the keystone for understanding of the disease; to this effect we are committed to the development of strategies to
ease the study of Xac. Genome sequencing and annotation of Xac revealed that ∼37% of the genome is composed of
hypothetical ORFs. To start a systematic characterization of novel factors encoded by Xac, we constructed integrative-vectors
for...
Tipo: Journal article
Palavras-chave: Citrus canker; Expression vectors; TAP-tag.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200518
Biological control of Phytophthora root rot of avocato with microorganisms grown in organic mulches
Provedor de dados: 58
Autores: Costa,Jefferson L. da S.; Menge,John A.; Casale,Willian L.
Organic mulches colonized with microbial biocontrol agents, termed bioenhanced mulches, were tested for their ability to reduce
Phytophthora root rot of avocado (Persea americana Mill.). Benomyl-resistant mutants of Gliocladium virens (KA 230-1) and
Trichoderma harzianum (KA 159.2) isolated from suppressive soils and selected as efficient antagonists of P. cinnamomi were
evaluated for their ability to colonize different mulches under controlled laboratory conditions. Sudangrass and a coarse yardwaste
were found to be better substrates than a fine yardwaste, woodwaste or rice hulls for biocontrol agents propagules production. The
most suitable conditions for colonization were an optimum temperature of 24°C, a moisture content of 20% for sudangrass and
30%...
Tipo: Journal article
Palavras-chave: Phytophthora cinnamoni; Gliocladium virens; Trichoderma harzianum; Avocado.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400002
Performance comparison between broilers positive and negative for antibodies against the chicken
anemia virus
Provedor de dados: 58
Autores: Rubin,Lauricio Librelotto; Ávila,Luiz Antônio Faccenda de; Ribeiro,Andréa Machado Leal; Wald,Vera; Canal,Cláudio
Wageck.
The chicken anemia virus (CAV) is present in virtually every country investigated, Brazil including. The aim of this study was to
determine what the difference in performance is between positive (progeny of breeders vaccinated or with natural infection) and
negative broilers to the presence of antibodies against the CAV in currently intensive raising systems. As a result, it was observed
that negative broilers were significantly heavier than positive broilers. Negative males had a final weight 5.43% higher than
positive males. There was no significant difference among different treatments in relation to parameters as mortality and feeding
conversion. These study indicated that the presence of antibodies against CAV in broilers - may it be through...
Tipo: Journal article
Palavras-chave: Chicken anemia virus; Performance; Broiler; Vaccine; Antibody.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500030
Optimization of the production of mycorrhizal inoculum on substrate with organic fertilizer
Provedor de dados: 58
Autores: Coelho,Ieda R; Pedone-Bonfim,Maria VL; Silva,Fábio SB; Maia,Leonor C.
The system for production of inoculum of arbuscular mycorrhizal fungi (AMF) using sand and vermiculite irrigated with nutrient
solution is promising. However, organic amendments added to the substrate can stimulate sporulation of AMF and replace the
nutrient solution. The aim of this study was to maximize the production of AMF (Acaulospora longula, Claroideoglomus
etunicatum, Dentiscutata heterogama and Gigaspora albida) using selected organic substrates (vermicompost, coir dust and
Tropstrato) together with sand and vermiculite. The production of spores varied among the tested AMF and according to the
organic source added to the substrate. The vermicompost promoted higher sporulation of A. longula in relation to the other AMF
and substrates. The...
Tipo: Journal article
Palavras-chave: AMF; Biofertilizer; Vermicomposto; Inoculum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400007
Microcolony detection in thin layer culture as an alternative method for rapid detection of
Mycobacterium tuberculosis in clinical samples
Provedor de dados: 58
Autores: Silva,Pedro Eduardo Almeida da; Wiesel,Fernanda; Boffo,Maria Marta Santos; von Groll,Andréa; Mattos,Ivo Gomes
de; Mejia,Glória; Robledo,Jaime.
Tuberculosis remains one of the major causes of mortality worldwide. The early detection of new cases is an important goal in the
program of tuberculosis control. Several methodologies for rapid and accurate laboratorial diagnosis have been developed,
however, some of these techniques are expensive or cumbersome, making their implementation in low-income regions unfeasible.
In this study, the thin layer culture method was compared with conventional culture method and it was observed that it provides
earlier results and a presumptive species identification, being adequate alternative method for rapid laboratory diagnosis.
Tipo: Journal article
Palavras-chave: Tuberculosis; Rapid diagnosis; Mycobacterium tuberculosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300007
Total lipids and fatty acids of strains of Metarhizium anisopliae
Provedor de dados: 58
Autores: Pupin,Antonio Marcos; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico; Roberts,Donald Wilson.
Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were
compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var. majus were similar in percentages of total lipids.
Tukey test for average of lipid content in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media
showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid
production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety
anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p= 1%)
with the highest...
Tipo: Journal article
Palavras-chave: Lipids; Fatty acids; Metarhizium anisopliae; Entomopathogenic fungi; Microbial control.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200012
Effect of ultrasound on survival and growth of Escherichia coli in cactus pear juice during storage
Provedor de dados: 58
Autores: Cruz-Cansino,Nelly del Socorro; Reyes-Hernández,Isidro; Delgado-Olivares,Luis; Jaramillo-Bustos,Diana Pamela;
Ariza-Ortega,José Alberto; Ramírez-Moreno,Esther.
Abstract The aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of
Escherichia coli inoculated into cactus pear juices (green and purple). Total soluble solids, pH, titratable acidity, and the kinetics
of E. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5 min) were
evaluated over 5 days. Total inactivation was observed in both fruit juices after 5 min of ultrasound treatment at most amplitude
levels (with the exception of 60% and 80%). After one and two days of storage, the recovery of bacteria counts was observed in
all cactus pear juices. Ultrasound treatment at 90% amplitude for 5 min resulted in non-detectable levels of E. coli in...
Tipo: Journal article
Palavras-chave: Ultrasound; Growth; Escherichia coli; Cactus pear juice; Storage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200431
Formulation of economical microbial feed using degraded chicken feathers by a novel Streptomyces
sp: mitigation of environmental pollution
Provedor de dados: 58
Autores: Ramakrishnan,Jayapradha; Balakrishnan,Hariram; Raja,Selvaraj Thirupathi Kumara; Sundararamakrishnan,Natarajan;
Renganathan,Sadagoban; Radha,Venkatesh Nagarajan.
A new Streptomyces sp. IF 5 was isolated from the feather dumped soil and found to have a tremendous keratinase activity. The
strain enabled the degradation of the chicken feathers very effectively in 60 h. The 16S rRNA sequence of 1474 bp long was
submitted to the National centre for Biotechnological information. The keratinolytic activity in the culture medium was 1181
U/ml. The release and analyses of sulphydryl groups in the culture medium evident the degradation activity by the Streptomyces
sp. IF 5. The idea of the present study was to use the degraded chicken feathers as the substrate for the growth and cultivation of
microorganisms. We have designed a very economical culture medium that includes the usage of some basal salts alone and
degraded...
Tipo: Journal article
Palavras-chave: Keratinase activity; Streptomyces sp; Microbial feed; Chicken feather.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300001
Different resistance patterns of reference and field strains of Brucella abortus
Provedor de dados: 58
Autores: Miranda,Karina L.; Dorneles,Elaine M. S.; Poester,Fernando P.; Martins Filho,Paulo S.; Pauletti,Rebeca B.;
Lage,Andrey P.
The aim of this study was to evaluate the growth of the B. abortus reference strains and field isolates on media containing
different inhibitor agents. Reference strains were seeded on tryptose agar containing: i-erythritol (1.0 mg/mL), fuchsin (20
μg/mL and 80 μg/mL), thionin (2.5 μg/mL and 10 μg/mL), rifampicin (200
μg/mL) and safranin O (200 μg/mL). Field isolates were tested only on media containing i-erythritol,
rifampicin and thionin. Furthermore, each suspension was also inoculated on tryptose agar incubated in air, to test its ability to
grow without CO2. Sensitivity to fuchsin was similar among reference strains evaluated. Growth of S19, 544 and 2308 but not
RB51 were inhibited on media...
Tipo: Journal article
Palavras-chave: B. abortus; Thionin; I-erythritol; Rifampicin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100265
Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
Provedor de dados: 58
Autores: Rall,Vera Lúcia Mores; Rall,Ricardo; Aragon,Lina Casale; Silva,Márcia Guimarães da.
We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV)
broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella Agar (SS), Brilliant Green Agar
(BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella
(CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT
broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with
less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23
(79.3%) of the 29 positive...
Tipo: Journal article
Palavras-chave: Salmonella; Poultry; Plating media; Selective enrichment.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200009
Effects of growth temperature on polystyrene adhesion of Pseudomonas aeruginosa ATCC 27853
Provedor de dados: 58
Autores: Cappello,Simone; Guglielmino,Salvatore P.P.
The importance of avoiding bacterial adhesion to plastic medical devices is recognized. To better understand the interaction
between bacteria and surface, we studied the influence of different growth temperatures (15, 30 and 47ºC) on the polystyrene
adhesion capacity of Pseudomonas aeruginosa ATCC 27853.
Tipo: Journal article
Palavras-chave: Pseudomonas aeruginosa; Temperature; Hydrophobicity; Adhesion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300001
Role of brown-rot fungi in the bioremoval of azo dyes under different conditions
Provedor de dados: 58
Autores: Ali,Naeem; Hameed,Abdul; Ahmed,Safia.
The present study is vital to the understanding of bioremediation of structurally different azo dyes by some unusual Brown-rot
fungi. Bioremoval of each dye (20 mg l-1) was tested in two different culture media under static and shaking conditions by taking
inocula from different fungi. Fungal strains showed varying dyes removal abilities, though considerable high in case of Acid Red
(AR) 151(di-azo) as compared to Orange (Or) II (mono-azo). With an exception of Aspergillus tereus SA3, all the fungal isolates
showed higher removal of dyes in SDB. Under static condition, the maximum decolorizing fungal strains were; Aspergillus flavus
SA2 (67%) and Alternaria spp. SA4 (57%) in AR 151, while Penicillium spp. (34 and 33 %) in Orange II, in SDB and STE,...
Tipo: Journal article
Palavras-chave: Azo dyes; Bio-removal; Bio-decolorization; Culture conditions; Fungi.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400009
Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region
Provedor de dados: 58
Autores: Guadarrama-Mendoza,P.C.; Valencia del Toro,G.; Ramírez-Carrillo,R.; Robles-Martínez,F.; Yáñez-Fernández,J.;
Garín-Aguilar,M.E.; Hernández,C.G.; Bravo-Villa,G.
Two native Pleurotus spp. strains (white LB-050 and pale pink LB-051) were isolated from rotten tree trunks of cazahuate
(Ipomoea murucoides) from the Mexican Mixtec Region. Both strains were chemically dedikaryotized to obtain their symmetrical
monokaryotic components (neohaplonts). This was achieved employing homogenization time periods from 60 to 65 s, and 3 day
incubation at 28 °C in a peptone-glucose solution (PGS). Pairing of compatible neohaplonts resulted in 56 hybrid strains which
were classified into the four following hybrid types: (R1-n xB1-n, R1-n xB2-1, R2-n xB1-n and R2-n xB2-1). The mycelial
growth of Pleurotus spp. monokaryotic and dikaryotic strains showed differences in texture (cottony or floccose), growth (scarce,
regular or abundant),...
Tipo: Journal article
Palavras-chave: Pleurotus; Mycelial growth; Mycelial morphology; Hybrid strains; Monokaryotization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300016
Selection and characterization of brivudin resistant bovine herpesvirus type 5
Provedor de dados: 58
Autores: Brum,Mário Celso Sperotto; Santos,Cyndia Mara Bezerra dos; Weiblen,Rudi; Flores,Eduardo Furtado.
Bovine herpesvirus type 5 (BoHV-5) is the agent of meningoencephalitis, an important disease of cattle in South America. The
neuropathogenesis of BoHV-5 infection is poorly understood and most previous research focused on the role of envelope
glicoproteins in neurovirulence. Thymidine kinase (TK) is a viral enzyme necessary for virus replication in neurons and, therefore,
represents a potential target for virus attenuation. The selection and characterization of BoHV-5 variants resistant to the
nucleoside analog brivudin (BVDU), which selects TK-defective viruses is here described. Several BVDU-resistant clones were
obtained after multiple passages in tissue culture in the presence of BVDU and one clone (BoHV-5/R-27) was further
characterized. The selected...
Tipo: Journal article
Palavras-chave: BoHV-5; Thymidine kinase; Drug resistant; Brivudin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100019
The reaction of dental pulp to Escherichia coli lipopolysaccharide and Enterococcus faecalis
lipoteichoic acid
Provedor de dados: 58
Autores: Oliveira,Laudimar Alves de; Barbosa,Sérgio Valmor.
This research evaluates the effects of the lipopolysaccharides (LPS) from Escherichia coli and lipoteichoic acid (LTA) from
Enterococcus faecalis on dental pulp. These molecules are components of the Gram-negative and Gram-positive bacteria cell wall,
respectively. Ten dogs were used in the experiment. Inoculation in surgically opened pulp and coronal restoration with glass
ionomer was the method chosen. The evaluation times were 1, 7, 15, 30 and 60 days. The results showed that the LPS and LTA, at
150 µg/ml, produced a negative interference in the pulp leading to destruction. LTA caused less damage than LPS.
Tipo: Journal article
Palavras-chave: Lipopolysaccharide; Endotoxin; Lipoteichoic acid; Dental pulp; Bacteria.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200018
Poly R decolorization and APPL production by Streptomyces violaceoruber and Streptomyces
spiroverticillatus
Provedor de dados: 58
Autores: Abou-Dobara,M.I.; Omar,N.F.
Two mesophilic streptomycetes (S. violaceoruber and S. spiroverticillatus) were selected to study their Poly R-478 decolorization
ability and lignocellulose solubilizing activity. Both strains were able to degrade Poly R-478 dye and ferulic acid during growth
on a minimal salts medium. The Poly R-478 decolorizing activities of both strains were induced by adding different carbon
sources to the culture media. S. violaceoruber could decolorize 63% of Poly R-478 after 24 h. Both strains could
solubilize straw and produce acid-precipitable polymeric lignin (APPL) with different efficiency. From the major extracellular
enzymes recovery of both strains on rice and wheat straw, we can predicate that the biodegradation process was partial indicating
a...
Tipo: Journal article
Palavras-chave: Streptomyces; APPL; Poly-R decolorization; Ferulic acid degradation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400008
Infleunce of pH on the partition of glucose-6-phosphate dehydrogenase and hexokinase in aqueous
two-phase system
Provedor de dados: 58
Autores: Silva,Daniel Pereira da; Pontes,Marcela Zanella Ribeiro; Souza,Maria Aparecida de; Vitolo,Michele; Silva,João Batista
de Almeida e; Pessoa-Junior,Adalberto.
Glucose-6-phosphate dehydrogenase (G6PDH) and hexokinase (HK) are important enzymes used in biochemical and medical
studies and in several analytical methods. Aqueous two-phase system (ATPS) formed by a polymer solution and an electrolyte
solution provides a method for the separation and purification of enzymes with several advantages, including biocompatibility and
easy scale up of the process. In this work, the effects of different pH values on the storage stability and partitioning behavior (K,
partition coefficient) of the enzymes G6PDH and HK from baker's yeast extract were investigated in ATPS. The results, obtained
from the 17.5% PEG 400 : 15.0% phosphate system, showed that when the pH was increased from 5.0 to 8.8, the K HK increased
26-fold and...
Tipo: Journal article
Palavras-chave: Aqueous two-phase systems; Partition coefficient; Protein purification; Hexokinase; Glucose 6-phosphate
dehydrogenase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300002
Comparative study between yeasts immobilized on alumina beads and on membranes prepared by
two routes
Provedor de dados: 58
Autores: Kiyohara,Pedro K.; Lima,Urgel de Almeida; Santos,Helena de Souza; Santos,Persio de Souza.
Alumina channeled beads and rough surface membranes prepared from aqueous sols of fibrillar pseudoboehmite are able to
immobilize yeasts for ethanol fermentation of sugar solutions. This paper describes comparative results of assays carried out with
yeasts immobilized onto alpha-alumina beads and membranes prepared under two different conditions of processing and firing.
The fermentation tests evaluated by the decrease of fermentable sugars, referred as Brix degrees per hour, indicated that the yeasts
immobilized on beads had similar performance, probably because their surfaces, even being morphologically different, presented
the same value of open porosity. One type of membrane (asymmetrical; precursor: pseudoboehmite; firing temperature 1,150ºC;
crystal...
Tipo: Journal article
Palavras-chave: Porous alumina; Channeled alumina; Immobilized yeast; Ethanol production.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200008
Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation
Provedor de dados: 58
Autores: Ramos,Aline de Souza; Fiaux,Sorele Batista; Leite,Selma Gomes Ferreira.
Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the
present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in
solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A
Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within
the experimental domain studied were (100 g support)-1: sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g;
inoculum concentration, 2.2 x 10(6) spores; moisture level, 55%. The temperature established was 28ºC. The fermentation under
the selected conditions led...
Tipo: Journal article
Palavras-chave: 6-pentyl-α -pyrone solid-state fermentation experimental design.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400022
Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application
Provedor de dados: 58
Autores: Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling;
Peng,Hongjun; Shi,Weifeng.
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli
BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with
serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases,
IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The
results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM
antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of
IgG antibodies in...
Tipo: Journal article
Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Provedor de dados: 58
Autores: Leite,D.C.A.; Balieiro,F.C.; Pires,C.A.; Madari,B.E.; Rosado,A.S.; Coutinho,H.L.C.; Peixoto,R.S.
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few
studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples
requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial
species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high
quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the
extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three
commercial DNA extraction...
Tipo: Journal article
Palavras-chave: Biochar; DNA extraction; PCR-DGGE; Microbial communities; DNA purity indices.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100023
Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates
in a university hospital located in the Northeast of Brazil
Provedor de dados: 58
Autores: Sousa Júnior,Francisco Canindé de; Néri,Gildelane da Silva; Silva,Ana Karine; Araújo,Bibiana Priscila Rodrigues
Câmara de; Guerra,Myrian Júlia de Paiva Dourado; Fernandes,Maria José de Britto Costa; Milan,Eveline Pipolo; Melo,Maria
Celeste Nunes de.
Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the
heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to
evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the
Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level
heterogeneous resistance strains. Both the 30 µg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be
good options for the detection of MRSA in the clinical laboratory.
Tipo: Journal article
Palavras-chave: MRSA; Methicillin resistance; Heterogeneous resistance; Cefoxitin; PBP2a.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200009
Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates
Provedor de dados: 58
Autores: Hou,Xiang-hua; Song,Xiu-yu; Ma,Xiao-bo; Zhang,Shi-yang; Zhang,Jia-qin.
Klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. Selective pressure, the extensive use
of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the
emergence of multidrug-resistant (MDR) K. pneumoniae. Here, we examined the occurrence, phenotypes and genetic features of
MDR K. pneumoniae isolated from patients in intensive care units (ICUs) at the First Affiliated Hospital of Xiamen University in
Xiamen, China, from January to December 2011. Thirty-eight MDR K. pneumoniae strains were collected. These MDR K.
pneumoniae isolates possessed at least seven antibiotic resistance determinants, which contribute to the high-level resistance of
these...
Tipo: Journal article
Palavras-chave: Klebsiella pneumoniae; Multidrug resistance; Molecular characterization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300759
Microbiological evaluation of precooked goat "buchada"
Provedor de dados: 58
Autores: Costa,Roberto Germano; Santos,Neube Michel dos; Medeiros,Ariosvaldo Nunes de; Queiroga,Rita de Cássia Ramos do
Egypto; Madruga,Marta Suely.
The objective of the present work was to evaluate the microbiological characteristics of the precooked goat "buchada", a typical
dish in North East of Brazil, composed mainly by goat viscera such as heart, lungs, liver, spleen, intestines, stomach and blood.
Samples of "buchada" produced at the different areas of the State of Paraíba, Brazil, presented a high number of aerobic
mesophilic bacteria, with values ranging from 5.5 to 6.9 log10 CFU/g. All samples presented total coliforms, and 96.6% presented
fecal coliforms, with values between 2.3 and 5.0 log10 NMP/g. The occurrence of Staphylococcus aureus was observed in one
sample only, at a proportion of 4.0 log10 CFU/g. Salmonella was absent in all samples. Water activity was around 0.98 and pH
close to...
Tipo: Journal article
Palavras-chave: Organs; Viscera; Hygienic-sanitary conditions; Buchada.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300029
Sources of poultry meat contamination with thermophilic Campylobacter before slaughter
Provedor de dados: 58
Autores: Franchin,Paulo Rogério; Aidoo,Kofi E.; Batista,Cleide Rosana Vieira.
The possible sources of Campylobacter spp. in poultry meat before slaughter were studied by examining samples of feathers,
cloaca swabs, litter swabs, transport coops, rinse water from coop washing equipment, and chicken breast supports in the slaughter
line just before stunning. The samples were collected from 8 broiler houses and from 8 different producers, from a poultry
integration system in southern Brazil. The study was carried out over a 12-month period, and each broiler house was sampled in
three consecutive flocks, for a total of 24 flocks/broiler house. Campylobacter was found in 79.2% of the feather samples,
followed by cloacal swabs (75.0%) and transport coop (50.0%), litter (37.5%), breast support (33.3%) and coop rinse water
(25.0%) samples....
Tipo: Journal article
Palavras-chave: Campylobacter; Poultry processing.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200011
Arcobacter butzleri and A. cryaerophilus in human, animals and food sources, in southern Chile
Provedor de dados: 58
Autores: Fernandez,Heriberto; Villanueva,Maria Paz; Mansilla,Ingrid; Gonzalez,Mario; Latif,Fadua.
The isolation frequency of Arcobacter species in children with diarrhea, fowls, mammals and food of avian and marine origin was
established. In all the samples it was possible to isolate Arcobacter species corresponding 201 (39.4%) to A. butzleri and 24 (4.7)
to A. cryaerophilus. Both species were simultaneously isolated in 19 (3.7%) being A. butzleri the most frequently isolated species.
Tipo: Journal article
Palavras-chave: Arcobacter diarrhea; Reservoirs; Food.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100145
Effect of arbuscular mycorrhizal fungi on young vines in copper-contaminated soil
Provedor de dados: 58
Autores: Ambrosini,Vítor Gabriel; Voges,Joana Gerent; Canton,Ludiana; Couto,Rafael da Rosa; Ferreira,Paulo Ademar Avelar;
Comin,Jucinei José; Melo,George Wellington Bastos de; Brunetto,Gustavo; Soares,Cláudio Roberto Fonsêca Sousa.
Abstract High copper (Cu) levels in uprooted old vineyard soils may cause toxicity in transplanted young vines, although such
toxicity may be reduced by inoculating plants with arbuscular mycorrhizal fungi (AMF). The objective of this study was to
evaluate the effects of AMF on the plant growth, chlorophyll contents, mycorrhizal colonization, and Cu and phosphorus (P)
absorption in young vines cultivated in a vineyard soil contaminated by Cu. Commercial vineyard soil with high Cu levels was
placed in plastic tubes and transplanted with young vines, which were inoculated with six AMF species (Dentiscutata heterogama,
Gigaspora gigantea, Acaulospora morrowiae, A. colombiana, Rhizophagus clarus, R. irregularis) and a control treatment on
randomized blocks...
Tipo: Journal article
Palavras-chave: Mycorrhizae; Trace elements; Phosphorus; Vitis sp.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401045
Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
Provedor de dados: 58
Autores: Sá,Rafael A. de; Castro,Frederico A.V. de; Eleutherio,Elis C.A.; Souza,Raquel M. de; Silva,Joaquim F.M. da;
Pereira,Marcos D.
Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial,
antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae
as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against
oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆,
gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during
60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25
µg/mL of alcoholic...
Tipo: Journal article
Palavras-chave: Propolis; Antioxidant; Oxidative stress; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300051
Beijerinckia derxii stimulates the viability of non-N2-fixing bacteria in nitrogen-free media
Provedor de dados: 58
Autores: Barbosa,Heloiza R.; Thuler,Daniela Strauss; Shirakawa,Márcia Aiko; Miyasaka,Natália R. S.
The interactions between the nitrogen-fixing microorganism Beijerinckia derxii with two non-diazotrophic bacteria, either
Escherichia coli or a facultative sulphur-oxidizing chemolitotroph, were studied in mixed cultures. Direct and indirect contact
between B. derxii and E. coli were tested. B. derxii increased CFU numbers and/or maintained the viability of the
non-diazotrophic bacteria, but neither growth nor nitrogenase activity of the nitrogen-fixing bacterium were affected by either
partner.
Tipo: Journal article
Palavras-chave: Beijerinckia derxii; Coculture; Viability.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300004
Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some
potentially pathogenic Aspergillus species
Provedor de dados: 58
Autores: Carmo,Egberto Santos; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Sousa,Frederico Barbosa de.
Cinnamomum zeylanicum Blume is known for a wide range of medicinal properties. This study aimed to assess the interference
of C. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. The essential
oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth
inhibition zones. MIC50 and MIC90 values were 40 and 80 µL/mL, respectively. 80, 40 and 20 µL/mL of the oil strongly
inhibited the radial mycelial growth of A. niger, A. flavus and A. fumigatus along 14 days. 80 and 40 µL/mL of the oil caused a
100% inhibition of the fungal spore germination. Main morphological changes observed under light microscopy provided by the
essential oil in the...
Tipo: Journal article
Palavras-chave: Aspergillus; Cinnamomum zeylanicum Blume; Essential oil; Antifungal activity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100021
Etiology of canine otitis media and antimicrobial susceptibility of coagulase-positive Staphylococci in
Fortaleza city, Brazil
Provedor de dados: 58
Autores: Oliveira,Lis Christina de; Leite,Carlos Artur Lopes; Brilhante,Raimunda Sâmia N.; Carvalho,Cibele Barreto M.
This study evaluated the middle ear microbiota and antimicrobial susceptibility patterns from strains isolated from dogs with otitis
media. A total of 62 dogs obtained from Zoonoses Control Center of Fortaleza City Ceará State / Brazil were studied over a
10-month period (August/2003 to June/2004). Of the total, 46.8% (n=30) of the animals were positive for otitis media and the
infection was monomicrobial in 76.6% of them. The most frequent isolated agents were coagulase-positive Staphylococci
(CPS-55%) and Pseudomonas sp (10%). For S. intermedius (n=13) and S. aureus subsp aureus (n=9), respectively, the greater
resistance rates were observed using penicillin G (30.76% and 44.44%), ampicillin (7.69% and 44.44%), erythromycin (23.07%
and 44.44%),...
Tipo: Journal article
Palavras-chave: Otitis media; Dogs; Staphylococci; Antimicrobials.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200009
Early changes in arbuscular mycorrhiza development in sugarcane under two harvest management
systems
Provedor de dados: 58
Autores: Azevedo,Lucas Carvalho Basilio de; Stürmer,Sidney Luiz; Lambais,Marcio Rodrigues.
Sugarcane (Saccharum spp.) is grown on over 8 million ha in Brazil and is used to produce ethanol and sugar. Some sugarcane
fields are burned to facilitate harvesting, which can affect the soil microbial community. However, whether sugarcane pre-harvest
burning affects the community of arbuscular mycorrhizal fungi (AMF) and symbioses development is not known. In this study,
we investigated the early impacts of harvest management on AMF spore communities and root colonization in three sugarcane
varieties, under two harvest management systems (no-burning and pre-harvest burning). Soil and root samples were collected in
the field after the first harvest of sugarcane varieties SP813250, SP801842, and RB72454, and AMF species were identified based
on spore...
Tipo: Journal article
Palavras-chave: Glomeromycota; Slash-and-burn; Sugarcane harvest management; No-burning.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300032
Optimization of biodegradable plastic production on sugar cane molasses in Enterobacter sp. SEL2
Provedor de dados: 58
Autores: Naheed,Nighat; Jamil,Nazia.
Contaminated environments have a large number of bacteria which can accumulate PHA as their energy reserves. Out of 54
isolated bacterial strains from three groups of contaminated sites 48 were found PHA positive. The sites were grouped on the basis
of the type of carbon sources i.e. sugars, fatty acids and much diverse type. Strains MFD5, MFD11, UML3, USL2, SEL2, SEL3,
SEL10 and PFW1 produced 69.9 ± 0.29, 75.27 ± 0.45, 65.43 ± 0.1, 72.54 ± 0.27, 76.61 ± 0.28, 61.81 ± 0.05, 71.16 ± 0.09 and
74.92 ± 0.5 percent of PHA to their constant cell weight (CCW) respectively in PHA detection media supplemented with 2%
glucose. Molasses, whey, crumbs hydrolysate and palm oil were checked as inexpensive carbon sources. Molasses alone could
supply the required...
Tipo: Journal article
Palavras-chave: Enterobacter sp. SEL2; Contaminated environments; Molasses.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200008
Solving ethanol production problems with genetically modified yeast strains
Provedor de dados: 58
Autores: Abreu-Cavalheiro,A.; Monteiro,G.
The current world demand for bioethanol is increasing as a consequence of low fossil fuel availability and a growing number of
ethanol/gasoline flex-fuel cars. In addition, countries in several parts of the world have agreed to reduce carbon dioxide emissions,
and the use of ethanol as a fuel (which produces fewer pollutants than petroleum products) has been considered to be a good
alternative to petroleum products. The ethanol that is produced in Brazil from the first-generation process is optimized and can be
accomplished at low cost. However, because of the large volume of ethanol that is produced and traded each year, any small
improvement in the process could represent a savings of billions dollars. Several Brazilian research programs are investing in...
Tipo: Journal article
Palavras-chave: Brazilian ethanol production; Yeast improvement; Resistance to stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300001
Photodynamic inactivation of four Candida species induced by photogem®
Provedor de dados: 58
Autores: Dovigo,Lívia Nordi; Pavarina,Ana Cláudia; Ribeiro,Daniela Garcia; Adriano,Cynthia Sanchez; Bagnato,Vanderlei
Salvador.
This study evaluated the in vitro susceptibility of C. albicans, C. dubliniensis, C. tropicalis and C. krusei to photodynamic therapy
(PDT) induced by Photogem® and light emitting diode (LED). Suspensions of each Candida strain were treated with three
photosensitizer (PS) concentrations (10, 25 and 50 mg/L) and exposed to 18.0, 25.5 and 37.5 J/cm² LED light fluences
(λ ~ 455 nm). Control suspensions were treated only with PS concentrations, only exposed to the LED light fluences or
not exposed to LED light or PS. Sixteen experimental conditions were obtained and each condition was repeated three times.
From each sample, serial dilutions were obtained and aliquots were plated on Sabouraud Dextrose Agar. After incubation of plates
(37 ºC for 48...
Tipo: Journal article
Palavras-chave: Photochemotherapy; Candida; Hematoporphyrin Derivative.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100009
New host record of five Flavobacterium species associated with tropical fresh water farmed fishes
from North India
Provedor de dados: 58
Autores: Verma,Dev Kumar; Rathore,Gaurav.
Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated
with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy
freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three
farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were
identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and
phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different
formally...
Tipo: Journal article
Palavras-chave: Flavobacterium; Fish; Species diversity; 16S rDNA; Phylogenetic analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400969
Bacterial consortium for copper extraction from sulphide ore consisting mainly of chalcopyrite
Provedor de dados: 58
Autores: Romo,E.; Weinacker,D.F.; Zepeda,A.B.; Figueroa,C.A.; Chavez-Crooker,P.; Farias,J.G.
The mining industry is looking forward for bacterial consortia for economic extraction of copper from low-grade ores. The main
objective was to determine an optimal bacterial consortium from several bacterial strains to obtain copper from the leach of
chalcopyrite. The major native bacterial species involved in the bioleaching of sulphide ore (Acidithiobacillus ferrooxidans,
Acidithiobacillus thiooxidans, Leptospirillum ferrooxidans and Leptospirillum ferriphilum) were isolated and the assays were
performed with individual bacteria and in combination with At. thiooxidans. In conclusion, it was found that the consortium
integrated by At. ferrooxidans and At. thiooxidans removed 70% of copper in 35 days from the selected ore, showing significant
differences...
Tipo: Journal article
Palavras-chave: Bioleaching; Acidithiobacillus ferrooxidans; Acidithiobacillus thiooxidans.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200032
Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated
in Porto Alegre, Brazil
Provedor de dados: 58
Autores: Bender,Eduardo André; Freitas,Ana Lúcia Peixoto de; Reiter,Keli Cristine; Lutz,Larissa; Barth,Afonso Luís.
In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In
the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered
from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by
conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level
aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion.
The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was:
2.5% to ampicillin,...
Tipo: Journal article
Palavras-chave: Enterococcus; Resistance; HLAR; PFGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300035
Fluorescent Pseudomonads associated with the rhizosphere of crops: an overview
Provedor de dados: 58
Autores: Botelho,Gloria R.; Mendonça-Hagler,Leda Cristina.
Environmental concerns have led to the need of sustainable use of natural resources. The conventional agriculture caused
considerable impacts on soils and waters. It is important to change certain agricultural managements to environmental cleaner
techniques. The sustainable agriculture has pointed many approaches and techniques to reduce environmental impact. One of
those strategies is the utilization of the soil microbiota to induce plant growth, control of plant diseases and biodegradation of
xenobiotic compounds. Studies on the relationship between roots and microbiota are essential to achieve viable agricultural
applications. These studies indicate that one of the most abundant microorganisms in the rhizosphere (area around the roots) is
fluorescent...
Tipo: Journal article
Palavras-chave: Plant growth promoting; Biological control; Fluorescent Pseudomonads; Rhizosphere.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400001
Concomitant production of detergent compatible enzymes by Bacillus flexus XJU-1
Provedor de dados: 58
Autores: Niyonzima,Francois N.; More,Sunil S.
A soil screened Bacillus flexus XJU-1 was induced to simultaneously produce alkaline amylase, alkaline lipase and alkaline
protease at their optimum levels on a common medium under submerged fermentation. The basal cultivation medium consisted of
0.5% casein, 0.5% starch and 0.5% cottonseedoil as an inducer forprotease, amylase, and lipase, respectively. The casein also
served as nitrogen source for all 3 enzymes. The starch was also found to act as carbon source additive for both lipase and
protease. Maximum enzyme production occurred on fermentation medium with 1.5% casein, 1.5% soluble starch, 2% cottonseed
oil, 2% inoculum size, initial pH of 11.0, incubation temperature of 37 °C and 1% soybean meal as a nitrogen source supplement.
The analysis of time...
Tipo: Journal article
Palavras-chave: Bacillus flexus; Alkaline enzyme; Concomitant production; Optimization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300020
Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine
mastitis
Provedor de dados: 58
Autores: Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap.
This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex
PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin
resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was
performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to
be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were
slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of
59 strains...
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009
Optimization of growth medium for protease production by Haloferax Lucentensis VKMM 007 by
response surface methodology
Provedor de dados: 58
Autores: Manikandan,Muthu; Kannan,Vijayaraghavan; Velikonja,Blagajana Herzog; Pašic,Lejla.
The production of halophilic thermostable protease by Haloferax lucentensis VKMM 007 was optimized using a statistical
approach. In accordance with factorial design, soluble starch, gelatin, KCl and MgSO4 were selected among 27 variables tested.
Next, a second-order quadratic model was estimated and optimal medium concentrations were determined based on quadratic
regression equation generated by model. These were 5.14 g L-1 of KCl, 6.57 g L-1of MgSO4, 9.05 g L-1of gelatin and 5.27 g
L-1of soluble starch in high salts media supplemented with 0.5% (w/v) of beef extract and peptone, respectively. In these optimal
conditions, the obtained protease concentration of 6.80 U mL-1 was in agreement with the predicted protease concentration and
was further improved...
Tipo: Journal article
Palavras-chave: Cultivation media; Haloferax lucentensis; Response surface methodology; Optimization; Halophilic protease.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200050
Aerobic bacterial microflora of Broad-snouted caiman (Caiman latirostris) oral cavity and cloaca,
originating from Parque Zoológico Arruda Câmara, Paraíba, Brazil
Provedor de dados: 58
Autores: Silva,J.S.A.; Mota,R.A.; Pinheiro Júnior,J.W.; Almeida,M.C.S.; Silva,D.R.; Ferreira,D.R.A.; Azevedo,J.C.N.
The objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's
samples, collected from Broad-snouted caiman (Caiman latirostris), born and bred in captivity at Parque Zoológico Arruda
Câmara, João Pessoa, Paraíba, Brazil. The most common bacteria were Staphylococcus sp. (14.74%), Corynebacterium sp.
(13.68%), Escherichia coli (13.68%) and Shigella sp. (11.58%), and the less common were Citrobacter sp. (1.05%), Klebsiella
pneumoniae (1.05%) and Salmonella sp. (1.05%).This emphasizes the importance of these microorganisms' participation in
infectious processes (sepsis) and injuries caused by crocodilians.
Tipo: Journal article
Palavras-chave: Caiman latirostris; Cloaca; Crocodilians; Microbiology; Oral cavity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100034
Detection of Staphylococcus aureus with an intermediate profile to vancomycin (VISA) isolate from
Santa Maria, RS
Provedor de dados: 58
Autores: Marques,Jaciane Baggiotto; Dalmolin,Tanise Vendruscolo; Bonez,Pauline Cordenonsi; Agertt,Vanessa Albertina;
Campos,Marli Matiko Anraku de; Santos,Roberto Christ Vianna.
Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA
through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the
presence of VISA in clinical isolates in the city of Santa Maria-RS.
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Vancomycin; Microdilution.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100040
The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe
FM4-64
Provedor de dados: 58
Autores: Lv,Yuntong; Xia,Zhikuan; Wang,Wenling.
Abstract The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The
amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within
growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake
experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers
were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that
Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin
polymerization, leads...
Tipo: Journal article
Palavras-chave: Trichosporon asahii; Spitzenkörper; Polarized growth; Cytochalasin D.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100266
Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.
Provedor de dados: 58
Autores: Thomas,Roshmi; Janardhanan,Anju; Varghese,Rintu T.; Soniya,E.V.; Mathew,Jyothis; Radhakrishnan,E.K.
Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical,
catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the
synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for
nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the
present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been
attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was
found...
Tipo: Journal article
Palavras-chave: Biosynthesis; Silver nanoparticles; Ochrobactrum anhtropi; Purification; Antibacterial activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400012
Degradation of 2,4-D herbicide by microorganisms isolated from Brazilian contaminated soil
Provedor de dados: 58
Autores: Silva,Tatiane M.; Stets,Maria I.; Mazzetto,André M.; Andrade,Fabiana D.; Pileggi,Sônia A. V.; Fávero,Paulo R.;
Cantú,Marcelo D.; Carrilho,Emanuel; Carneiro,Paulo I.B.; Pileggi,Marcos.
The aim of this work was to isolate microorganisms from Brazilian soil contaminated with 2,4-D herbicide, and analyze the
efficiency for 2,4D degradation, using high-performance liquid chromatography (HPLC). Serratia marcescens and Penicillium sp
had never been reported as able to degrade 2,4-D. The isolated strains represent a great potential for bioremediation.
Tipo: Journal article
Palavras-chave: Bioremediation Biodegradation 2; 4-dichlorophenoxyacetic acid.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300026
Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and
vegetables in São Paulo, Brazil
Provedor de dados: 58
Autores: Carvalho,Aline Feola de; Silva,Daniela Martins da; Azevedo,Sergio Santos; Piatti,Rosa Maria; Genovez,Margareth
Elide; Scarcelli,Eliana.
Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in
animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence
of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the
international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study
were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables
(lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent...
Tipo: Journal article
Palavras-chave: Campylobacter spp.; Cytolethal distending toxin (CDT); Broiler carcasses; Vegetables; Multiplex-PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300005
Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy
dairy cows in Inner Mongolia, China
Provedor de dados: 58
Autores: Huasai,Simujide; Chen,Aorigele; Wang,Chun-jie; Li,Yu; Tongrige,Bai.
Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex
PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting
for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution
detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%)
was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.
Tipo: Journal article
Palavras-chave: E. coli; Virulence gene; Multiplex PCR; Serogroup; Healthy dairy cow.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013
Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone
Provedor de dados: 58
Autores: Kurbanoglu,Esabi Basaran; Ozdal,Murat; Ozdal,Ozlem Gur; Algur,Omer Faruk.
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged
culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper
(Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA
classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile
(FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was
investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of
prodigiosin. A concentration...
Tipo: Journal article
Palavras-chave: Prodigiosin; Serratia marcescens; Ram horn peptone; Agricultural waste.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200631
The occurrence of molds, yeasts and mycotoxins in pre-cooked pizza dough sold in Southern Rio
Grande do Sul
Provedor de dados: 58
Autores: Pinho,Beatriz Helena; Furlong,Eliana Badiale.
The quality of pre-cooked pizza dough was investigated by assessing the occurrence of molds, yeasts and mycotoxins. Random
sampling of commercial pre-cooked pizza cakes was done in different stores in the cities of Rio Grande and Pelotas, RS, between
1996 and 1997. The products were analysed on the sampling day and after storage at room (22-30ºC) or refrigerated temperature
(7ºC) following the shelf life stated by the manufacturer (25,30 and 45 days). The results showed that mold and yeast
contamination was frequently above the maximum limits (10³CFU/g-1) established by Brazilian guide lines, even in samples kept
at refrigerated temperatures up to the end of shelf life. Although no mycotoxin contamination was detected, a strain of the
Penicillium genus,...
Tipo: Journal article
Palavras-chave: Pre-cooked pizza dough; Fungi; Mycotoxins.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200007
ERRATA - vol.42 no.1 São Paulo Jan./Mar. 2011
Provedor de dados: 58
Tipo: Journal article
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200051
Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in
Escherichia coli
Provedor de dados: 58
Autores: Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying.
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological
characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with
other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the
complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced.
The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with
a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11
of glycosyl...
Tipo: Journal article
Palavras-chave: Xylanase; Gene cloning; Fusariumsp.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049
Improved diagnosis of central nervous system tuberculosis by MPB64-Target PCR
Provedor de dados: 58
Autores: Dil-Afroze,; Mir,Abdul Waheed; Kirmani,Altaf; Shakeel-ul-Rehman,; Eachkoti,Rafiqa; Siddiqi,Mushtaq A.
Central nervous system (CNS) tuberculosis is a serious clinical problem, the treatment of which is sometimes hampered by
delayed diagnosis. Clearly, prompt laboratory diagnosis is of vital importance as the spectrum of disease is wideand abnormalities
of the cerebrospinal fluid (CSF) are incredibly variable. Since delayed hypersensitivity is the underlying immune response,
bacterial load is very low. The conventional bacteriological methods rarely detect Mycobacterium tuberculosis in CSF and are of
limited use in diagnosis of tuberculous meningitis (TBM). This double blind study was, therefore, directed to the molecular
analysis of CNS tuberculosis by an in-house-developed PCR targeted for amplification of a 240bp nucleotidesequence coding for
MPB64...
Tipo: Journal article
Palavras-chave: CNS tuberculosis; Tuberculous meningitis (TBM); Intracranial tuberculomas; PCR; Mycobacterium
tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200002
Effect of sub-minimum inhibitory concentrations of ciprofloxacin, amikacin and colistin on biofilm
formation and virulence factors of Escherichia coli planktonic and biofilm forms isolated from human
urine
Provedor de dados: 58
Autores: Wojnicz,Dorota; Tichaczek-Goska,Dorota.
The aim of this study was to determine the effect of subinhibitory concentrations (sub-MICs) of ciprofloxacin, amikacin and
colistin on biofilm formation, motility, curli fimbriae formation by planktonic and biofilm cells of E. coli strains isolated from the
urine of patients with various urinary system infections. Quantification of biofilm formation was carried out using a microtiter
plate assay and a spectrophotometric method. Bacterial enumeration was used to assess the viability of bacteria in the biofilm.
Curli expression was determined by using YESCA agar supplemented with congo red. Using motility agar the ability to move was
examined. All the antibiotics used at sub-MICs reduced biofilm formation in vitro, decreased the survival of bacteria, but...
Tipo: Journal article
Palavras-chave: Biofilm; Sub-MIC; Ciprofloxacin; Amikacin; Colistin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100037
Production of xylan-degrading enzymes by a Trichoderma harzianum strain
Provedor de dados: 58
Autores: Cacais,André O.Guerreiro; Silveira,Fabiane Q. de Paula; Ferreira Filho,Edivaldo Ximenes.
Trichoderma harzianum strain 4 produced extracellular xylan-degrading enzymes, namely beta-xylanase, beta-xylosidase and
alpha-arabinofuranosidase, when grown in liquid medium cultures containing oat spelt xylan as inducer. Cellulase activity was not
detected. The pattern of xylan-degrading enzymes induction was influenced by the form of xylan present in the medium. They
were detected in different incubation periods. Electrophoretic separation of the proteins from liquid culture filtrates by
SDS-PAGE showed a variety of bands with high and low molecular weights.
Tipo: Journal article
Palavras-chave: Xylan; T. harzianum; Xylanolytic activity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200014
Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp.
isolated from Brazilian foods
Provedor de dados: 58
Autores: Gomes,Bruna C.; Esteves,Carolina T.; Palazzo,Izabel C.V.; Darini,Ana Lúcia C.; Franco,Bernadette D.G.M.;
Martinis,Elaine C.P. de.
We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian
foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained
for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of
the isolates.
Tipo: Journal article
Palavras-chave: Enterococcus spp.; Identification; API 20 STREP; PCR multiplex; Foods.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400007
Xenobiotics enhance laccase activity in alkali-tolerant γ-proteobacterium JB
Provedor de dados: 58
Autores: Singh,Gursharan; Batish,Mona; Sharma,Prince; Capalash,Neena.
Various genotoxic textile dyes, xenobiotics, substrates (10 µM) and agrochemicals (100 µg/ml) were tested for enhancement of
alkalophilic laccase activity inγ-proteobacterium JB. Neutral Red, Indigo Carmine, Naphthol Base Bordears and
Sulphast Ruby dyes increased the activity by 3.7, 2.7, 2.6 and 2.3 fold respectively. Xenobiotics/substrates like p-toluidine,
8-hydroxyquinoline and anthracine increased it by 3.4, 2.8 and 2.3 fold respectively. Atrazine and trycyclozole pesticides
enhanced the activityby 1.95 and 1.5 fold respectively.
Tipo: Journal article
Palavras-chave: γ -proteobacterium laccase xenobiotics dyes agrochemicals.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100004
PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS
USING AN INEXPENSIVE MEDIUM
Provedor de dados: 58
Autores: Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile;
Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de.
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was
investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum
activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium
sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC.
Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The
optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and
8.4 and 8.0 for...
Tipo: Journal article
Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010
Commercially laid eggs vs. discarded hatching eggs: contamination by Salmonella spp
Provedor de dados: 58
Autores: Kottwitz,Luciana B.M.; Leão,Joice Aparecida; Back,Alberto; Rodrigues,Dalia dos P.; Magnani,Marciane;
Oliveira,Tereza C.R.M. de.
Salmonella enterica is frequently associated with outbreaks of human salmonellosis, and products of avian origin, such as eggs
and chicken meat, are the main vehicles of its transmission. The present study describes the occurrence of different serovars of
Salmonella enterica and phagotypes of S. enterica serovar Enteritidis in eggs destined for human consumption. Four thousand
eggs obtained from commercial egg laying farms and one thousand discarded hatching eggs from broiler farms, which were
acquired at farmers' markets and informal shops, were analyzed. Salmonella spp. was isolated from 52.0% of the discarded
hatching eggs, in which the predominant serovar was Enteritidis (84.6%), and the predominant Salmonella Enteritidis phagotype
(PT) was PT7...
Tipo: Journal article
Palavras-chave: Discarded eggs; Salmonella Enteritidis; Antimicrobial resistance; Salmonellosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200004
Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from
honey stomach of honeybee
Provedor de dados: 58
Autores: Tajabadi,Naser; Mardan,Makhdzir; Saari,Nazamid; Mustafa,Shuhaimi; Bahreini,Rasoul; Manap,Mohd Yazid Abdul.
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were
collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two
isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA
of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and
entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most
closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely
related to...
Tipo: Journal article
Palavras-chave: Honey bee; Honey stomach; Lactobacillus; Probiotic; 16S rDNA gene sequences.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300008
A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida
albicans and its closely related Candida dubliniensis
Provedor de dados: 58
Autores: Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da.
Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close
similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C.
dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and
discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically
digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of
other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C.
dubliniensis. Thus two species were...
Tipo: Journal article
Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification;
Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004
Antimicrobial activity of Anonna mucosa (Jacq.) grown in vivo and obtained by in vitroculture
Provedor de dados: 58
Autores: de Souza Barboza,Thiago José; Ferreira,Andréa Fonseca; de Paula Rosa Ignacio,Ana Claudia; Albarello,Norma.
Brazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture
offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances.
Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit antimicrobial activities. The widespread
use of antibiotics has led to an increase in multidrug-resistant bacteria, which represents a serious risk of infection. In view of this
problem, the aim of this work was to evaluate the antibacterial potential of extracts of A. mucosa obtained by in vitro techniques
and also cultured under in vivo conditions. Segments from seedlings were inoculated onto different culture media...
Tipo: Journal article
Palavras-chave: Antibacterial properties; Annonaceae; Medicinal plant; Callus cultures; Cell suspension cultures.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300785
Pathogenicity characteristics of stocked and fresh yeasts strains
Provedor de dados: 58
Autores: Chaves,Guilherme Maranhão; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo.
In order to evaluate the pathogenicity of yeasts of medical importance, 15 strains stocked in mineral oil at the URM Culture
Collection of the Department of Mycology, Biological Sciences Centre, Federal University of Pernambuco and 15 fresh strains
isolated from AIDS patients interned at the Clinical Hospital, Centre of Health Sciences, Federal University of Pernambuco, were
selected. As pathogenicity characteristics, the ability to grow at 37ºC and production of phospolipase and proteinase were tested.
All samples grew at 37ºC and exhibited proteinase activity. However when tested on solid medium, the proteinase activity was
negative. Among 15 stocked samples, 13 (86.66%) were phospholipase positive and two (13.34%) were phospholipase negative.
From the...
Tipo: Journal article
Palavras-chave: Yeasts; Pathogenicity; Temperature; Phospholipase; Proteinase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300003
New bioemulsifiers produced by Candida lipolytica using D-glucose and babassu oil as carbon sources
Provedor de dados: 58
Autores: Vance-Harrop,Mabel H.; Gusmão,Norma B. de; Campos-Takaki,Galba Maria de.
Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil
and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was
used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium
sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept
under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were
0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158
units, respectively....
Tipo: Journal article
Palavras-chave: Bioemulsifier; Candida lipolytica; Seawater; Babassu oil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200006
Prevalence and phenotypic characterization of Enterococcus spp. isolated from food in Brazil
Provedor de dados: 58
Autores: Camargo,Carlos Henrique; Bruder-Nascimento,Ariane; Lee,Sarah Hwa In; Fernandes Júnior,Ary; Kaneno,Ramon;
Rall,Vera Lúcia Mores.
We evaluated the frequency of enterococci from food and found 95.2% of positivity, being E. faecium and E. faecalis the most
frequent species. High-level streptomycin resistance was observed, as well as gelatinase and hemolysis activity, showing the
potential role of environmental strains as reservoir of virulence and resistance traits.
Tipo: Journal article
Palavras-chave: Enterococcus spp.; Food; Antimicrobial resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100016
Multiple organ dysfunction syndrome associated with Mycoplasma pneumoniae infection
Provedor de dados: 58
Autores: Zhai,Shu-Bo; Cao,Dian-Bo; Xu,Hui-; Li,Xin-Xiang; Yang,Si-Rui.
In this study, we report one case of a three-year-old boy infected with Mycoplasma pneumonia (MP) and presenting concomitant
multiple organ damage of the heart, kidney, lung and liver, among others, together with a brief review for the diagnosis and
treatment of MP infection with multiple organ dysfunction syndrome (MODS).
Tipo: Journal article
Palavras-chave: Mycoplasma pneumonia (MP); Multiple organ dysfunction syndromes (MODS).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100025
Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation
Provedor de dados: 58
Autores: Kwiecinska-Piróg,Joanna; Bogiel,Tomasz; Skowron,Krzysztof; Wieckowska,Ewa; Gospodarek,Eugenia.
Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm
formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total
number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in
Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and
qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride) and CV (crystal violet) application. The obtained results
confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the...
Tipo: Journal article
Palavras-chave: Proteus mirabilis; Biofilm; Colorimetric methods.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400037
Comparison of teicoplanin disk diffusion and broth microdilution methods against clinical isolates of
Staphylococcus aureus and S. epidermidis
Provedor de dados: 58
Autores: Camargo,Carlos Henrique; Mondelli,Alessandro Lia; Bôas,Paulo José Fortes Villas.
The CLSI M100-S19 document has recommended the disuse of vancomycin disks for staphylococci and informed that studies on
the action of teicoplanin in disk-diffusion testing should be performed. We describe the comparison of two methods, disk
diffusion and broth microdilution, for determining teicoplanin susceptibility in clinical isolates of staphylococci. Overall results
showed an aggregation rate of 96.8%; Staphylococcus aureus showed total agreement while S. epidermidis showed 93.8% of
agreement. According to these local results, disk diffusion can still be employed to teicoplanin susceptibility determination for
staphylococci in our institution.
Tipo: Journal article
Palavras-chave: Teicoplanin; Susceptibility tests; S. aureus; S. epidermidis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400003
Bacteria isolated from Korean black raspberry vinegar with low biogenic amine production in wine
Provedor de dados: 58
Autores: Song,Nho-Eul; Cho,Hyoun-Suk; Baik,Sang-Ho.
Abstract A high concentration of histamine, one of the biogenic amines (BAs) usually found in fermented foods, can cause
undesirable physiological side effects in sensitive humans. The objective of this study is to isolate indigenous Acetobacter strains
from naturally fermented Bokbunja vinegar in Korea with reduced histamine production during starter fermentation. Further, we
examined its physiological and biochemical properties, including BA synthesis. The obtained strain MBA-77, identified as
Acetobacter aceti by 16S rDNA homology and biochemical analysis and named A. aceti MBA-77. A. aceti MBA-77 showed
optimal acidity % production at pH 5; the optimal temperature was 25 °C. When we prepared and examined the BAs synthesis
spectrum during the...
Tipo: Journal article
Palavras-chave: Biogenic amines; Histamine; Acetic acid bacteria; Vinegar; Wine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200452
Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes)
against Oncometopia facialis (signoret) (hemiptera: cicadellidae)
Provedor de dados: 58
Autores: Pria Júnior,Wolney Dalla; Lacava,Paulo Teixeira; Messias,Claudio Luiz; Azevedo,João Lúcio; Lacava,Pedro
Magalhães.
Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa
and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the
sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension
containing 5 X 10(7) conidia mL-1. Adults captured in the field were treated in groups of 10 in a total of 11 replications pe