Download Consulta: subject:"Biotechnology" Registros recuperados: 913

Consulta: subject:"Biotechnology"
Registros recuperados: 913
Data/hora: 08/06/2017 23:35:54
Melhoramento genético de trigo via linhagens duplo-haplóides, na Embrapa Trigo, no período
1995-1998.
Provedor de dados: 119
Autores: SCHEEREN, P. L.; FERNANDES, M. I. B. de M.; BRAMMER, S. P.; ZANATTA, A. C. A.; IORCZESKI, E. J.; SÓ e
SILVA, M.; MOREIRA, J. C. S.; SOUSA, C. N. A. de; DEL DUCA, L. de J. A.; SILVA, S. D. dos A. e; DOTTO, S. R.;
BRUNETTA, D.; PANDOLFI, V.; MARTINS, L. F.; GABRIEL, N. E.; LUZA, J.
Visando a aceleração no processo de obtenção de linhagens de trigo e a melhoria das condições de seleção, em material
geneticamente fixo (homozigoto), foi implementada a produção de novas linhagens duplo-haplóides (DH), via gimnogenese
(DHM), no projeto melhoramento genético de trigo para o Brasil, conduzido na Embrapa Trigo, em Passo Fundo, Rio Grande do
Sul. Com a utilização dessa metodologia, que permite a criação de linhagens geneticamente uniformes, em menos de dois anos, a
partir do cruzamento trigo X milho, o tempo para a criação de novas linhagens e cultivares foi reduzido de dois a quatro anos,
considerando para comparação, respectivamente, o método convencional e o método rápido, que utiliza o avanço de geração no
verão. No período 1995-1998...
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Biotechnology.
Ano: 1999
URL: http://www.alice.cnptia.embrapa.br/handle/doc/825291
A unique avenue to control drug resistant pathogens: Experimental evidences
Provedor de dados: 17
Autores: Sribir Sen.
Drug resistant bacterial pathogens are increasing in the environment due to multiple reasons. If any drug resistant pathogen grows
in a host, the host will face death treat because there is no way to resist their growth in the host system. Eventuality is death in
almost all cases. Reports on the mechanisms of drug resistance in bacteria are many. However, there is no solution for these cases.
The present work is providing an important understanding to fight against drug resistant pathogens and that can be used in
gastrointestinal diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4934/version/1
An integrated solution for rapid biosensing combining linker free binding, freeze drying and high
sensitivity ellipsometric detection
Provedor de dados: 17
Autores: Yongbai Yin; David R. McKenzie; Keith Fisher; Chuan Guo; Neil Nosworthy; Marcela M. Bilek.
A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma
polymer to achieve linker-free binding of biorecognition molecules that allows the sensor surface to be freeze dried for long shelf
life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost,
versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the
methodology were demonstrated using three antigen-antibody pairs.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6764/version/1
Generation of "backbone" free, low transgene copy plants by launching
T-DNA from the _Agrobacterium_ chromosome
Provedor de dados: 17
Autores: Heiko Oltmanns; Bronwyn Frame; Lan-Ying Lee; Susan Johnson; Bo Li; Kan Wang; Stanton Gelvin.
In both applied and basic research, _Agrobacterium_-mediated transformation is commonly used to introduce genes into plants.
We investigated the effect of three _Agrobacterium tumefaciens_ strains and five T-DNA origins of replication on transformation
frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector
("backbone") into the genome of _Arabidopsis thaliana_ and _Zea mays_. Launching T-DNA from the
_picA_ locus of the _Agrobacterium_ chromosome increases the frequency of single transgene integration events and almost
eliminates the presence of vector backbone sequences in transgenic plants. Along with novel _Agrobacterium_ strains we have
developed, our findings are useful...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3497/version/1
Effect of Escherichia coli endotoxin on Archachatina marginata haemolymph coagulation system
Provedor de dados: 17
Autores: Musa Salawu; Oyelola Oloyede; Adenike Oladiji; Nasiru Muhammad; Musa Yakubu.
The effect of _E. coli_ endotoxin on the heamolymph coagulation response of _Archachatina marginata_ was studied. Heamocyte
Lysate(HL), Haemocyte Lysate Supernatant(HLS) and Haemocyte Lysate Debris (HLD) were exposed to _Escherichia coli_
endotoxin. Controls were prepared with endotoxin-free water(<0.025 EU/ml). The differential protein coagulation was
estimated in each mixture. Fractions of the haemolymph exposed to endotoxin produced higher protein coagulates than
endotoxin-free fractions when incubated at 37 °C for 1 h (p<0.05). The results showed significantly higher
(p<0.05) concentrations of protein coagulated when HL/plasma mixture were used than when either fraction was used.
At a ratio 1:1 of HL:Plasma, highest...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4552/version/1
Skin Typing and High Resolution MRI of Human Skin: Multiple Contrast Approach for Image
Characterization
Provedor de dados: 17
Autores: Rakesh R. Sharma.
Background and objectives:
characteristics
are
reported

The ex vivo magnetic resonance microimaging (MRM) image
in
human
skin
excised
samples.

Design
and
methods:
Human excised skin samples were imaged using custom coil placed inside 500 MHz NMR imager
for high resolution microimaging. Skin in vivo- and ex vivo MRI images were processed for characterization of different skin
structures. Contiguous cross-sectional T1-weighted 3D spin echo MRI, T2-weighted 3D spin echo MRI and proton density images
were compared with skin histopathology and NMR peaks. In all skin specimens, epidermis and dermis thickening and hair follicle
size was measured using MRM. 
Results:...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4161/version/2
Diamond nanowires, a new approach towards next generation electrochemical gene sensor platforms
Provedor de dados: 17
Autores: Nianjun Yang; Hiroshi Uetsuka; Jiuhong Yu; Eiji Osawa; Norio Tokuda; Christoph Nebel.
A novel bio-sensing platform is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned
diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Ultra-hard
vertically aligned diamond nanowires are electrochemically modified to bond phenyl linker-molecules to their tips which provide
mesospacing for DNA molecules on the transducer. The nano-wires are generated by reactive ion etching of metallically boron
doped atomically smooth single crystalline CVD diamond. Surface properties are characterized by atomic force, scanning electron
and scanning tunneling microcopy. Electro- and biochemical sensor properties are investigated using cyclic and differential
pulse...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1714/version/1
Genetic variability among _Coleus sp_ studied by RAPD banding pattern analysis
Provedor de dados: 17
Autores: Muthusamy Govarthanan; Arunapriya S; Guruchandar A; Selvankumar T; Gnanasekaran N; Manoharan Koildhasan.
Genetic improvement of the medicinal plants depends upon the existence, nature and extent of the genetic variability available for
manipulation. Genetic analysis with RAPD markers has been extensively used to determine genetic diversity among _Coleus sp_
and to identify the best quality for human consumption for its medicinal purpose. The objectives of the present study were to
assess molecular variation among _C.amboinicus_, _C.aromaticus_ and _C.forskohlii_. and to determine the level of genetic
similarity among them. We performed random amplification of polymorphic DNA (RAPD) analysis on three strains of _Coleus
sp_. Random primers were used for the PCR. Electrophoresis on denaturing acrylamide gels improved RAPD reproducibility and
increased the band...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5631/version/1
Open Notebook Science: Perspectives from a newbie
Provedor de dados: 17
Autores: Cameron Neylon.
My group is using an electronic lab notebook based on a Blog format that is being developed in collaboration with the group of
Professor Jeremy Frey. Here I discuss how this led to the adoption of an open notebook science approach in my group as well as
some of the consequences, both positive and negative, of adopting such an approach.
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1130/version/1
Skin Typing and High Resolution MRI of Human Skin: Multiple Contrast Approach for Image
Characterization
Provedor de dados: 17
Autores: Rakesh R. Sharma.
Background and objectives:
characteristics
are
reported

The ex vivo magnetic resonance microimaging (MRM) image
in
human
skin
excised
samples.

Design
and
methods:
Human excised skin samples were imaged using custom coil placed inside 500 MHz NMR imager
for high resolution microimaging. Skin in vivo- and ex vivo MRI images were processed for characterization of different skin
structures. Contiguous cross-sectional T1-weighted 3D spin echo MRI, T2-weighted 3D spin echo MRI and proton density images
were compared with skin histopathology and NMR peaks. In all skin specimens, epidermis and dermis thickening and hair follicle
size was measured using MRM. 
Results:...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4161/version/1
Newer Methods of Nanoparticle Synthesis: Nitroimidazole properties with Nanometal oxides in
Polymer Cages as Drug-Biomarker Monitors
Provedor de dados: 17
Autores: Rakesh Sharma.
Nitroimidazoles are radiosensitizers and hypoxia detecting chemosensitizers. Intially nitroimidazoles were single dose antibiotic
drugs.
Recently,
nitroimidazole
derivatives
have
emerged
as
multifunctional
“drug-biomarker
monitors” chemical compounds with importance in treatment of tumors, monitoring hypoxia and safer imaging
contrast agents to monitor the therapeutic progress. Additionally, the number of nitroimidazole derivatives is growing and
identified their importance as multifunctional nanoparticles. The nanoparticle synthesis scheme of nitroimidazole was proposed as
nitroimidazole caged with paramagnetic metal oxide inside polymer coating and labeled with marker radiolebels or biomolecules.
We report the step by step...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3952/version/1
21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound
Nanoparticles
Provedor de dados: 17
Autores: Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH. After injecting
SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart
including cardiac arteries, veins, cordate tendons attached with valves.
The measurement accuracy of ventricles, aorta,
vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2643/version/2
Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10
degrees Centigrade
Provedor de dados: 17
Autores: Uttam Datta; M Chandrasekar; Manik Lal Hembram; Raju Dasgupta.
Electrolyte free mediums prepared with soybean lecithin-glycerol,Coenzyme Q10–glycerol and soybean
lecithin-Coenzyme Q10-glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degree
centigrade, wrapped with aluminium foils along with testis and freezed at -10 degree centigrade.Spermatozoan characters were
evaluated 7 and 21 days after thawing at 38.5 degree centigrade in a water bath for 5 min. Spermatozoan characters were
diminished gradually and significantly (P<0.001, P<0.05) between the mediums and observation days.
Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive
motility, livability, hypo-osmotic swelling...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3377/version/1
Screening Lactic Acid Bacteria for Antimicrobial Compound Production
Provedor de dados: 17
Autores: Khalisanni Khalid; Lee Hung Kiong.
Lactic Acid Bacteria was known as potential probiotic used in food industries and dairy products and probable to produce
antimicrobial compound that inhibit variety of microorganisms. The objectives of the research are to determine the optimum
condition and glucose utilization in relation to antimicrobial compound production. Two species of Lactic Acid Bacteria namely
Lactococcus and Lactobacillus were used as probiotic. The Lactic Acid Bacteria were fermentated in different medium, initial
substrate pH and incubation temperature for the production of antimicrobial compound. The test organisms such as E.coli and
Salmonella were selected as test organisms. Amongst the two species of Lactic Acid Bacteria, Lactococcus produced the highest
amount of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3326/version/1
In-Vivo Lipidomics using Single-Cell Raman Spectroscopy
Provedor de dados: 17
Autores: Huawen Wu; Joanne Volponi; Ann Oliver; Atul Parikh; Blake Simmons; Seema Singh.
We describe a method for direct, quantitative, in vivo lipid profiling of oil producing microalgae using single-cell laser-trapping
Raman spectroscopy (LTRS). This approach is demonstrated in the quantitative determination of the degree of unsaturation and
transition temperatures of constituent lipids within microalgae. These properties are important markers for determining engine
compatibility and performance metrics of algal biodiesel. We show that these factors can be directly measured from a single living
microalgal cell held in place with an optical trap while simultaneously collecting Raman data. Cellular response to different
growth conditions is monitored in real time. Our approach circumvents the need for lipid extraction and analysis that is both...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4428/version/1
Co-detection: Ultra-reliable Nanoparticle-Based Electrical Detection of Biomolecules in the Presence
of Large Background Interference
Provedor de dados: 17
Autores: Yang Liu; Evangelyn Alocilja; Shantanu Chakrabartty.
Contrary to popular belief, we report that background interference in conductimetric biochips can be exploited using a novel
"co-detection" principle to significantly improve the reliability of detecting trace quantities of
biomolecules. The technique called "co-detection" exploits the non-linear redundancy amongst
synthetically patterned biomolecular logic circuits for deciphering the presence or absence of target biomolecules in a sample. In
this paper, we demonstrate the "co-detection" principle on gold-nanoparticle based conductimetric
soft-logic circuits which uses a silver-enhancement technique for signal amplification. Using co-detection, we have been able to
measure a 1000 times improvement in...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4389/version/1
A comparative study on the production of ethanol from lignocellulosic biomass by chemical and
biological method
Provedor de dados: 17
Autores: Shaheed SM; Rohit JP; Madhan R; Selvakumar K.
Ethanol derived from non-edible biomass is renewable and a clean source of energy. It is independent of the food industry and it is
economically feasible. The first generation biofuel or bioethanol is still not a very convenient source of energy as it prominently
depends on the availability of grains. The main objective of this work is to develop an industrious efficient process to produce
ethanol from lignocellulosic biomasses like wood and leaf in a lab scale. Two processes were compared. The first process
involved an alkaline pre-treatment of the powdered biomass followed by dilute acid hydrolysis. The second process involved an
alkaline treatment followed by direct hydrolysis of the biomass by use of a fungal species obtained from rotting wood....
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6579/version/1
Margarine products quality monitoring using reflectance UV-VIS-SWNIR spectroscopy
Provedor de dados: 17
Autores: Rui C. Martins; Vitor V. Lopes; António A. Vicente.
Margarine is a term that can indicate any of a wide range of butter substitutes. Due to the great diversity of the raw material,
margarine end-product quality characteristics are expected to be highly diversified. This article proposes the use of reflectance
UV-VIS-SWNIR spectroscopy to monitor the margarine end-product quality properties. The main effort in this work was the
development of a fast monitoring procedure to assess the quality of the manufactured margarines. 
The study
was performed on two margarine products: regular margarine (less than 80% fat) and reduced-fat margarine (less than 60% fat).
The nine product samples were collected during the production line normal operating conditions on different days. The samples
had the...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2293/version/1
Micro-magnetic resonance imaging (micro-MRI) study on the sepsis effected eyeball of zebrafish
Provedor de dados: 17
Autores: Bimal Kumar Sarkar; Wu Zen; Shangwu Ding; C. H. Hsu; Z.H. Wen; C. S. Lin; Chiranjib Chakraborty.
We have studied the normal and sepsis effected zebrafish eyeball with micro-magnetic resonance imaging (micro-MRI). T2
weighted image was studied and pixel-wise T2 maps were co registered among the normal and sepsis effected eye. From the
micro-image the sepsis effect in the eye has been demonstrated. The pixel-wise brightness distribution is not so scattered in the
normal eyeball image, whereas it is very scattered in case of sepsis affected eyeball image. Also the T2 mapping on the eye has
given valuable information that would be a potential tool for the study of diseased organ in the micro level. From T2 mapping, it
has shown that the T2 in normal eyeball have low values in comparison to the sepsis affected eyeball.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2694/version/1
Intestinal Electric Stimulation Accelerates Whole Gut Transit and Promotes Fat Excrement in
Conscious Rats
Provedor de dados: 17
Autores: Ying Sun; Jiande Chen.
*_Introduction:_* Intestinal electric stimulation (IES) is proposed as a potential tool for the treatment of morbid obesity. Our
previous study showed that IES with one pair of electrodes accelerated intestinal transit and decreased fat absorption in a segment
of the jejunum in the anesthetized rats. The aims of this study were to assess the effects of IES on the whole gut transit and fat
absorption in conscious rats, to examine the effects of multi-channel IES, and to explore the cholinergic mechanism behind the
effects of IES. 
*_Methods:_* Thirty-eight male rats implanted with serosal electrodes were randomized
into five groups: control without IES, 2/3 channel IES with short pulses, atropine and atropine plus IES. The whole gut transit...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2854/version/1
Causal Stability Ranking
Provedor de dados: 17
Autores: Daniel J. Stekhoven; Lars Hennig; Gardar Sveinbjörnsson; Izabel Moraes; Marloes H. Maathuis; Peter
Bühlmann.
Genotypic causes of a phenotypic trait are typically determined via randomized controlled intervention experiments. Such
experiments are often prohibitive with respect to durations and costs. We therefore consider inferring stable rankings of genes,
according to their causal effects on a phenotype, from observational data only. Our method allows for efficient design and
prioritization of future experiments, and due to its generality it is useable for a broad spectrum of applications.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6716/version/1
Rapid bioassay for evaluating enzyme production in fungal isolates from environmental sources
Provedor de dados: 17
Autores: Eleonora Bordo; Francesca Luziatellie; Silvia Crognale; Sara Marinari; Stefano Grego; Maurizio Ruzzi.
Fungal hydrolytic enzymes have a great potential due to the rapid development of enzyme technology and their industrial
applications. A fluorimetric microplate assay has been developed for measuring activity of β-D-exoglucanase,
β-D-glucosidase, α-D-glucosidase, β-N-acetyl-hexosaminidase, β-D-xylosidase
in diluted culture broth samples. The substrates used are conjugates of the highly fluorescent compounds 4-methylumbelliferone
(MUB) and thus product formation can be measured directly in the microplate without previous extraction and purification of the
product. The developed system was evaluated on eight fungal strains isolated from shrub species (Tamarix) that can be used in
phytoremediation. Depending...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6217/version/1
Where Is Scientific Evidence in Support of Refuge Size Reduction for Pyramided Bt Crops?
Provedor de dados: 17
Autores: Andrei Alyokhin.
Genetically modified plants expressing insecticidal Cry proteins originating from a soil-dwelling bacterium Bacillus thuringiensis
(Bt) provide a powerful tool for managing insect pests. Unfortunately, insect ability to develop resistance to insecticidal proteins
potentially jeopardizes its long-term efficiency. Review of registration materials submitted by plant biotech industry to the U.S.
Environmental Protection Agency and the existing scientific literature shows that currently available evidence in support of
reducing refuge for the pyramided genetically modified corn plants to 5% of the total crop area is essentially limited to predictions
of a single unpublished mathematical model developed “in-house” by the industry...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6563/version/1
Recent trends in US patent grants and issues to be considered
Provedor de dados: 17
Autores: George M. Garrity; Charles T. Parker.
The life sciences have changed radically since the Convention on Biological Diversity first opened for signatures in 1992.
Traditional organism-based approaches to discovery and use of genetic resources have been supplanted by molecular approaches.
Biodiversity prospecting is more likely to be a programmatic bioinformatics activity rather than an activity conducted by field
scientists. Access to genetic resources is no longer centered on a hunt for novel species. Rather, the hunt is for novel genes and
metabolic pathways that can be cloned into well-understood expression systems and readily scaled-up for industrial production.
Information about contemporary research, development, and manufacturing practices needs to be addressed, especially when
those...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4998/version/1
Genetic Analysis of ABO and Rh Blood Groups in Brahmin Population of Uttar Pradesh,
India”
Provedor de dados: 17
Autores: Vandana V. Rai.
A series of glycoprotein and glycolipids on the red blood cell surface constitute blood group antigens. These are A, B, AB and O
in ABO blood group system and Rh in rhesus blood group system. These antigens are genetically controlled. Certain diseases
have been shown to be associated with certain blood groups. In the present study an attempt is made to study the distribution of
ABO and Rh. (D) blood group systems among the Brahmin caste population of Uttar Pradesh State. A total of 200 unrelated
Brahmin individuals from Uttar Pradesh were studied for the phenotype and allele frequency distribution of ABO and Rh (D)
blood groups. The order of occurrence of ABO phenotypes is B>O>A>AB. The corresponding
allele frequencies of O, A,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5931/version/1
High energy-charged cell factory for heterologous protein synthesis
Provedor de dados: 17
Autores: Pil Kim; Hye Jung Kim; Yeong Deok Kwon; Sang Yup Lee.
Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli
to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide
biosynthesis. To investigate the effect of a high intracellular ATP concentration on heterologous protein synthesis, we studied the
expression of a foreign gene product, enhanced green fluorescence protein (eGFP), under control of the T7 promoter in E. coli
BL21(DE3) strain overexpressing PCK. This strain was able to maintain twice as much intracellular ATP and to express two times
more foreign protein than the control strain. These results indicate that a high energy-charged cell can be beneficial as a...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3953/version/1
Dual expression recombinase based (DERB) single vector system for high throughput screening and
verification of protein interactions in living cells
Provedor de dados: 17
Autores: JianPing Lu; Laura Beatty; Jehonathan Pinthus.
Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary
for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial,
however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of
protein interactions in living cells. The platform's series of Dual Expression Recombinase Based (DERB) destiny
vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of
interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1550/version/1
Deterministic Separation of Cancer Cells from Blood at 10 mL/min
Provedor de dados: 17
Autores: Kevin Loutherback; Joseph D'Silva; Liyu Liu; Amy Wu; Robert Austin; James Sturm.
Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients
with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and
possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate,
propagate and characterize viable CTCs and clusters. Here, we present a microfluidic device for mL/min flow rate,
continuous-flow capture of viable CTCs from blood using deterministic lateral displacement arrays. We show here that a
deterministic bump array can be designed such that it will isolate with efficiency greater than 85% CTCs over a large range in
sizes from...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6861/version/1
Nanotoxicity Assessment toward the Applications of Carbon Nanotubes as a Small Biomolecule
Carrier in Drug Delivery Systems
Provedor de dados: 17
Autores: Rakesh Sharma; Soonjo Kwon; R Baktur; Avdhesh Sharma.
Carbon Nanotube (CNT) materials have superior properties in electric current carrying capacity, thermal conductivity, and
thermal stability. Due to their structure with high aspect ratio, they have structural unusual toxicity and complicate safety issue in
a target tissue.
In optimized quantities with limited functionality, special type of CNT assembly such as
“buckyball” can be used as a potential drug carrier of bioactive molecules and display with increased
circulating time and acceptable functionality. We analyzed cytoxicity and inflammatory response following exposure of different
size, mass, shape, and functionality of CNTs. We monitored the transport across skin, physiological perturbation of transepithelial
electrical...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3948/version/1
Invasive monitoring of the clinical effects of high intra-abdominal pressure for insertion of the first
trocar.
Provedor de dados: 17
Autores: Octavio H. M. Hypolito; Joao-Luiz Azevedo; Fernanda
Afonso C. C. G. Machado; Susana A. Miyahira; Glicia
A. B. Gama; Otavio C. Azevedo; Otavio M. Becker Junior;
C. Azevedo; Bianca Marigliani; José F. Borborema; Linda
Bernardes; Gustavo P. S. Miguel.
Background: To analyze the effects of transitory, high intra-abdominal pressure on clinical, hemodynamic, blood gas and
metabolic parameters.

Methods: Sixty-seven laparoscopic patients were divided into
groups P12 (n = 30, maximum intra-abdominal pressure of 12 mmHg) and P20 (n = 37, maximum intra-abdominal pressure of 20
mmHg). Through radial artery cannulation, mean arterial pressure (MAP) was assessed and blood gas analysis – pH,
arterial oxygen tension (PaO2), arterial carbon dioxide tension (PaCO2), bicarbonate (HCO3) and base excess (BE)
– was performed. These parameters were evaluated in both groups at time point zero, before CO2 insufflation; at
time point one (TP1), when intra-abdominal...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6536/version/1
Cryopreservation of a whole liver
Provedor de dados: 17
Autores: Zohar Gavish; Amir Arav; Menahem Ben-Haim.
Preservation of vascularized organs such as the liver is limited to 24hrs. before destructive processes disqualify it for
transplantation. This narrow time window prevents surgeons from performing optimal pathogen screening and matching tests
which often lead to re-transplantation. Numerous problems are associated with viably freezing and thawing a whole liver:
complicated geometry, poor heat transfer, release of latent heat and the difficulty of generating a uniform cooling rate. Our past
success led us to apply our novel freezing technique to a larger solid organ, the liver. Whole livers were frozen/thawed using a
directional solidification apparatuses; viability was tested by means of integrity and functionality in vitro and in auxiliary liver...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1352/version/1
_In vivo_ photoacoustic molecular imaging with simultaneous multiple selective targeting using
antibody-conjugated gold nanorods
Provedor de dados: 17
Autores: Pai-Chi Li; Dar-Bin Shieh; Churng-Ren Wang; Chen-Wei Wei; Chao-Kang Liao; Ann-Ann Ding; Ya-Na Wu; Carolina
Poe; Suwen Jhan.
The use of gold nanorods for photoacoustic molecular imaging in vivo with simultaneous multiple selective targeting is reported.
The extravasation of multiple molecular probes is demonstrated, and used to probe molecular information of cancer cells. This
technique allows molecular profiles representing tumor characteristics to be obtained and a heterogeneous population of cancer
cells in a lesion to be determined. The results also show that the image contrast can be enhanced by using a mixture of different
molecular probes. In this study, HER2, EGFR, and CXCR4 were chosen as the primary target molecules for examining two types
of cancer cells, OECM1 and Cal27. OECM1 cells overexpressed HER2 but exhibited a low expression of EGFR, while Cal27
cells showed...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1687/version/1
Structural analysis of outer membrane beta-stranded porins using B-factor
Provedor de dados: 17
Autores: Abhishek Kumar; S Krishnaswamy.
Computational and statistical analysis has formed a large component of the biophysical efforts put forth to understand protein
structure and function, due to the diversity and complexity of their structure. Outer membrane proteins form a diverse and
complex set of proteins. Of these, porins that allow passage of molecules across the membrane interface have been analyzed here
from a biophysical and structural perspective. The objective of this study is to analyze the structural organization of porins using
atomic temperature factor as a parameter. Generally atomic temperature factors of molecules from crystal structures indicate the
degree of mobility or disorder seen in the crystal structure. As good crystal structures have lesser possibilities of errors...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2803/version/1
A quantitative model for efficient construction of lentiviral vectors with a unique clone site
Provedor de dados: 17
Autores: Gang Zhang; Anurag Tandon.
Lentiviral vectors (LVs) were a powerful tool for transgene expression in vivo and in vitro. However, the construction of LVs is
of low efficiency, due to the large sizes and lack of proper clone sites. Therefore, it is critical to develop efficient strategies for
cloning LVs. Here, we reported a combinatorial strategy to efficiently construct LVs using EGFP, hPlk2 wild type (WT) and
mutant genes as inserts. Firstly, site-directed mutagenesis (SDM) was performed to create BamH I site for the inserts; secondly,
pWPI LV was dephosphorylated after BamH I digestion; finally, the amounts and ratios of the insert and vector DNA were
optimized to increase monomeric ligation. Our results showed that the total percentage of positive clones was approximately...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6876/version/1
The legacy of bio-molecules as a bio-fertilizer: Context of single cell fertilizer Isolation and partial
characterization of an amylolytic bacterium
Provedor de dados: 17
Autores: Sribir Sen; Anwesha Banerjee; Sanghamitra Sanyal.
Repeated cultivation of crop plants is the reason for the depletion of nutrients in an agricultural land. Therefore, modern
procedures of agriculture cascaded with the addition of organic and inorganic fertilizers, the use of insecticides and pesticides, the
addition of proper water, etc. Various inorganic molecules are used as fertilizers. However, the use of organic manures is also in
practice. They have many roles such as improving soil porosity, air holding capacity, water holding capacity, structure, texture,
etc. Agricultural scientists suggest using organic molecules for many reasons. Bio-fertilizers of many kinds are used by farmers of
all nations. However, these fertilizers are unable to cause tremendous effects on the growth and development of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6774/version/1
Aqueous synthesis of CdTe quantum dot as biological fluorescent probe for monitoring methyl
parathion by fluoro-immunosensor
Provedor de dados: 17
Autores: Raghuraj Chouhan; Aaydha Vinayaka; Munna Thakur.
Bioconjugation of quantum dots (QDs) provide high resolution in biological fluorescent labelling as a result of physical and
optical properties of QDs. This intrinsic property of QDs can be made use of for sensitive detection of target analytes including
food and environmental monitoring. In this investigation, we report the bioconjugation of thiol stabilized CdTe QD for the
sensitive detection of methyl parathion (MP) at picogram level. The specificity in the analysis was attributed by highly specific
competitive immunological reactions between free MP and CdTe QD bioconjugated MP (MP-BSA-CdTe) for immobilized
anti-MP IgY antibodies in a simple flow injection system. We also report the possible resonance energy transfer phenomenon as a
result of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3451/version/1
Exploiting Nonlinear Recurrence and Fractal Scaling Properties for Voice Disorder Detection
Provedor de dados: 17
Autores: Max A. Little; Patrick E. McSharry; Stephen A. Roberts; Declan A. E. Costello; Irene M. Moroz.
Background: Voice disorders affect patients profoundly, and acoustic tools can potentially measure voice function objectively.
Disordered sustained vowels exhibit wide-ranging phenomena, from nearly periodic to highly complex, aperiodic vibrations, and
increased "breathiness". Modelling and surrogate data studies have shown significant nonlinear and
non-Gaussian random properties in these sounds. Nonetheless, existing tools are limited to analysing voices displaying near
periodicity, and do not account for this inherent biophysical nonlinearity and non-Gaussian randomness, often using linear signal
processing methods insensitive to these properties. They do not directly measure the two main biophysical symptoms of disorder:
complex...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/326/version/1
An intelligent liposome that may deliver drug molecules in a well controlled fashion
Provedor de dados: 17
Autores: Dumitru Popescu; Alin Gabriel Popescu.
The passage of molecules, especially large ones, through the cellular membrane is a very important problem for some
biotechnological applications, such as drug delivery. The appearance of pores in the lipid bilayer following some controlled
mechanisms may be an adequate and interesting way. Some pores, named stochastic pores, can appear due to structural and
dynamic properties of lipid bilayer, but others may be favored by mechanical tension induced by different ways. Recently, a
sequence of 30-40 pores was observed in the same vesicle, a pore at a time, which can appear in vesicles stretched by optical
induced mechanical tension. There are two very interesting biotechnological applications that require the increase of membrane
permeability: gene therapy...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1220/version/1
Single cell fertilizer (SCF): Evidence to prove that bio-molecules are potent nutrient for plant growth
Provedor de dados: 17
Autores: Sribir Sen; Amit Kr. Roy; Sayan Choudhury; Shibu Ghosh; Somenath Das; Sohini Roy; Rajib Ghosh; Dipankar Dey;
Deben Pathak; Sanjeev Upadhyay; Isita Chattopadhya.
Fertilizers of various kinds are used for the cultivation of crop plants for hyper production of plant based food materials. The study
used bio-molecules made in a bacterial cell. The experimental results showed tremendous effect on plant growth. These cellular
molecules were made by treating the bacterial cells with
lysozyme and protenase K. The
wet/weight was increased in
multiple folds compared to that of control sets. The fold of increase was 4.79 for rice, 2.77 for wheat, 1.89 for gram and 1.89 for
pea when bacterial cellular molecules were used as fertilizer.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2816/version/1
The Proteomics World is Flat: Dependence of Regulation upon Abundance
Provedor de dados: 17
Autores: David Good; Roman Zubarev.
The long-standing assumption that proteins divide into house-keeping and cell-specific groups was recently struck down after
discovering that the vast majority of expressed proteins are shared across cell lines. Here, we tested a related hypothesis that
abundant proteins are less regulated than low-abundance ones. Meta-analysis of published data revealed that protein regulation is
only weakly dependent upon the protein abundance, thus providing support to cell characterization by the
"top" proteome.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6412/version/1
Nucleic acid - protein fingerprints. Novel protein classification based on nucleic acid - protein
recognition
Provedor de dados: 17
Autores: Alexander Krylov; Renad Zhdanov.
Protein chemistry uses protein description and classification based on molecular mass and isoelectric point as general features.
Enzymes are also compared by enzymatic reaction constants, namely Km and kcat values. Proteins are also studied by binding to
different oligonucleotides. Here we suggest a simple experimental method for such a comparison of DNA binding proteins, which
we call "nucleic acid-protein fingerprints". The experimental design of the method is based on an use of
short oligonucleotides immobilized inside microarray of hydrogel cells - biochip. As a first stage, we solved a simple experimental
task: what is the shortest single strand oligonucleotide to be recognized by protein? We tested binding of oligonucleotides from
2...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6955/version/1
Substrate entering and product leaving trajectories predict an engulfing dynamic for the major
conformational change of the β-lactam acylase
Provedor de dados: 17
Autores: Xi Huang; Jun Yin; Wei-Hong Jiang; Zixin Deng; Guo-Ping Zhao.
It is still a major challenge to acquire insight into the conformational changes between the ground state and the transition state of
an enzyme, although conformational fluctuation within interconverting conformers has been widely investigated (1-4). Here, we
utilize different enzymatic reactions in b-lactam acylase to figure out the substrate/product trajectories in the enzyme, thereby
probing the overall conformational changes in transition state. First, an auto-proteolytic intermediate of cephalosporin acylase (EC
3.5.1.11) with partial spacer segment was identified. As a final proteolytic step, the deletion of this spacer segment was revealed
to be a first-order reaction, suggesting an intramolecular Ntn mechanism for the auto-proteolysis. Accordingly,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2514/version/1
Role of intellectual property rights in biotechnology and pharmaceutical industries
Provedor de dados: 17
Autores: Ajeet Ajeet.
The main objective of this review is to highlight and explore the inter-relationship and the functioning of the intellectual property
right in the pharmaceutical and biotechnology industry. The rising tide of patent applications can be witnessed globally in this
industry as the need for such protection and licensing has become imperative so as to safeguard the rights of the inventor and also
to encourage and promote new talents, inventions and innovations which can be a boon for the economy. The field of
biotechnology is an upcoming science which is still at the initial stage of establishing a foundation but it promises a revolution in
the fields of medicine, agriculture, pharmaceuticals and industrial sector amongst other sectors of the economy along...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/7065/version/1
Rapid pre-gel visualization of proteins with mass spectrometry compatibility
Provedor de dados: 17
Autores: Marco A. Mata-Gomez; Matthew T. Yasui; Robert Winkler.
Despite all of the prophecies of doom, gel electrophoresis is still prevalent in modern proteomic workflows. However, the
currently used protein staining methods represent a serious bottleneck for a quick subsequent protein analysis using mass
spectrometry. Substituting traditional protein stains by pre-gel derivatization with visible and mass spectrometry compatible
reagents eliminates several processing steps and drastically reduces the sample preparation time. A defined chemistry permits
seamless integration of such covalent protein staining methods into standardized bioinformatic pipelines. Using Uniblue A we
could covalently stain simple to complex protein samples within 1 minute. Protein profiles on the gels were not compromised and
MS/MS based...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/5163/version/1
The study on estimation of time since death with proton magnetic resonance spectroscopy under
different temperature
Provedor de dados: 17
Autores: Tiantong Yang; Li Zhenwei; Wu Yue; Liu Liang; Zheng Na.
Estimation of the time of death (post-mortem interval, PMI) is occasionally a critical issue in adjudication of a criminal or civil
action. But no objective and precise means has been derived for estimating PMI.One reason for this outcome may be the fact that
most of these methods attempted to characterize PMI by a few or even just one specific parameter's variable in
constant ambient temperature. It is crucial that we reduce or eliminate the effect of temperature variation in the estimation of PMI.
Here we show the first results by using proton magnetic resonance spectroscopy (1H-MRS) technique to determine the order of
degradation of certain substances, present in the brain tissue of a rabbit model, under different temperatures; our goal is to...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1363/version/1
Resveratrol Increases Intracellular NAD+ Levels Through Up regulation of The NAD+ Synthetic
Enzyme Nicotinamide Mononucleotide Adenylyltransferase
Provedor de dados: 17
Autores: Ross S. Grant.
Resveratrol is a polyphenol with major health benefits that is thought to operate through direct activation of the
'anti-aging'
enzyme
SIRT1.
However
recent
reports
have
challenged
this
'direct-activation' hypothesis, suggesting the mechanism by which resveratrol increases SIRT1 function is
still unknown. We report for the first time that resveratrol induces a dose dependent increase in activity of the NAD+ synthetic
enzyme nicotinamide mononucleotide adenylyl transferase (NMNAT1). Activation of NMNAT1 by resveratrol in cultured
primary human astrocytes and neurons increased NAD+ levels by up to 5 fold. As SIRT1 requires NAD+ as a substrate to perform
its gene silencing function, higher NAD+ levels will enhance SIRT1...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4421/version/1
A database of naturally occurring human urinary peptides and proteins for use in clinical
applications
Provedor de dados: 17
Autores: Petra Zürbig; Joshua Coon; Hartwig Bauer; Georg Behrens; Mohammed Dakna; Anna Dominiczak; Stephane
Decramer; Jochen Ehrich; Danilo Fliser; Moritz Frommberger; Arnold Ganser; Mark Giolami; Igor Golovko; David Good;
Wilfried Gwinner; Marion Haubitz; Stefan Herget-Rosenthal; Holger Jahn; George Jerums; Bruce Julian; Markus Kellmann;
Volker Kliem; Walter Kolch; Andrzej Krolewski; Mario Luppi; Ziad Massy; Michael Melter; Christian Neusüss; Jan
Novak; Karlheinz Peter; Kasper Rossing; Harald Rupprecht; Joost Schanstra; Eric Schiffer; Jens-Uwe Stolzenburg; Lise Tarnow;
Dan Theodorescu; Visith Thongboonkerd; Raymond Vanholder; Eva Weissinger; Harald Mischak; Philippe Schmitt-Kopplin.
Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical
proteomics. However, the lack of comparable datasets from large cohorts has greatly hindered development in this field. Here we
report the establishment of a high resolution proteome database of naturally occurring human urinary peptides and proteins ranging from 800-17,000 Da - from over 3,600 individual samples using capillary electrophoresis coupled to mass spectrometry,
yielding an average of 1,500 peptides per sample. All processed data were deposited in an SQL database, currently containing
5,010 relevant unique urinary peptides that serve as classifiers for diagnosis and monitoring of diseases, including kidney and...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1219/version/1
Co-transformation of Oil Palm Using Agrobacterium-Mediated Transformation
Provedor de dados: 17
Autores: Vu Le.
Agrobacterium-mediated transformation is being used as an alternative method for improving the conventional breeding. In this
study, immature embryos (IEs) of oil palm are chosen as explants for transformation. β-glucuronidase (gus) and green
fluorescence protein (GFP) were harbored by the two plasmids pCAMBIA 1304 and pCAMBIA 1302 respectively. The results
were obtained at least four days after transformation. The IEs placing in the N6O medium for 7 days were then co-cultivated with
A.tumefaciens suspension within 30 mins and then incubated in the dark for 1 day. In single transformation, GUS assay revealed
that about 64% transient transformation frequency was obtained for IEs two weeks after co-cultivation, GFP activity was recorded
as 41.67%...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/5441/version/1
Temperature factor analysis of outer membrane β-stranded porin crystal structures
suggests the pore is not uniformly rigid
Provedor de dados: 17
Autores: S. Krishnaswamy; Abhishek Kumar.
Outer membrane beta-stranded porins form a diverse and complex set of proteins which allow passage of molecules across the
membrane interface have been analyzed here from a biophysical and structural perspective using atomic temperature factors or
B-factors. Generally atomic temperature factors of molecules from crystal structures indicate the degree of mobility or disorder
seen in the crystal structure. Structures of six porins (four 16 stranded beta barrel porins and two 8 stranded beta barrel porins)
were taken from the PDB for the analysis based on resolution (better than 3.0 Å) and R-factor (< 0.23). The
residue distribution and mobility distribution was found to be characteristic of each of the porins. The mobility and residue...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2495/version/1
From aptamer-based biomarker discovery to diagnostic and clinical applications: an aptamer-based,
streamlined multiplex proteomic assay
Provedor de dados: 17
Autores: Stephan Kraemer; Jonathon D. Vaught; Christopher Bock; Larry Gold; Evaldas Katilius; Tracy R. Keeney; Nancy
Kim; Nicholas A. Saccomano; Sheri K. Wilcox; Dom Zichi; Glenn M. Sanders.
Recently, we reported an aptamer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless
transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science
applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust,
sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that
quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our
platform, further expanding the breadth of proteomic applications for a wide user community.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4642/version/1
Relationship of arterial and exhaled CO2 during elevated artificial pneumoperitoneum pressure for
introduction of the first trocar.
Provedor de dados: 17
Autores: Octavio H. M. Hypolito; Joao-Luiz Azevedo; Susana A. Miyahira; Fabiana Scarpelli; Otavio M. Becker Junior; Afonso
C. C. G. Machado; Otavio C. Azevedo; Glicia C. Azevedo; Bianca Marigliani; Jose F. Borborema; Linda Bernardes; Gustavo P.
S. Miguel.
The present study evaluated the correlation between arterial CO2
and exhaled CO2 during brief high-pressure
pneumoperitoneum. Patients were randomly distributed into two groups: P12 group (n=30) received a maximum intraperitoneal
pressure of 12mmHg, and P20 group (n=37) received a maximum intraperitoneal pressure of 20mmHg. Arterial CO2 was
evaluated by radial arterial catheter and exhaled CO2 was measured by capnometry at the following time points: before
insufflation, once intraperitoneal pressure reached 12mmHg , 5 minutes after intraperitoneal pressure reached 12mmHg for the
P12 group or 20mmHg for the P20 group, and 10 minutes after intraperitoneal pressure reached 12mmHg for the P12 group or
when intraperitoneal pressure had decreased from 20mmHg...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6538/version/1
Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of
infection and protective immune responses
Provedor de dados: 17
Autores: Yong Keun Park; Yoon Won Suck; Lee Seung Seok.
IL-12 is known to be an essential cytokine which appears to provide protective immunity against intracellular bacteria, such as
Salmonella. In this study, we investigated the possibility of developing a vaccine using IL-12 against virulent Salmonella. We
used the host defense system activated by cytokine IL-12. The highly virulent Salmonella strain (Salmonella typhimurium UK-1)
was transformed with cytokine-expressing plasmids. These live, wild-type pathogens were used as vaccine strains without
undergoing any other biological or genetic attenuating processes. The newly developed strains induced partial protection from
infections (30-40%). Of note, the interleukin-12 transformed pathogen was safe upon immunization with low doses (103 CFU),
induced IgG...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3413/version/1
A Paradigm for Biology's Next Revolution
Provedor de dados: 17
Autores: Fredric D. R. Young.
A recent essay on biology's next revolution described attributes of a future theory of biology that would replace post
hoc modeling with an interplay between quantitative prediction and experimental test, more characteristic of the physical
sciences1. The article suggested that flux from the environment whether it be energy, chemicals metabolites, or genes is a defining
characteristic of life. The essay also suggests that an interdisciplinary approach to collective phenomena centered on statistical
mechanics and dynamical systems theory will be required to make use of this perspective. Over the last 30 years we have
developed an approach to drug discovery and translational research that is based on a paradigm for complex systems modeling
that fits...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1770/version/1
Milk is for Children, Colostrum silage is for calves.
Provedor de dados: 17
Autores: Mara Helena Saalfeld; Daniela Brayer Pereira; Kathleen Krüguer Silveira; Marcia Gularte; Julia Valente;
Renata Schramm; Fábio Leivas Leite.
One sixth of the world population is starving. In the meantime, producers from all over the world daily disdain billions of liters of
bovine colostrum, which is seen as rich in nutrients, immunoglobulin and bioactive substances. The milk is the most expensive
component in the final costs of calves breeding. Considering the impossibility of substituting the milk to feed the calf, different
ways to use the colostrum have been studied however with controversial results. We have developed colostrum silage. This
product is economical and possible to store in the environment for up to eighteen months. Being efficient for calf breeding,
yielding income and profit to the dairy business. The colostrum silage keeps the necessary physicochemical characteristics for...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6553/version/1
21 Tesla MRI Microscopy of Mice Kidney, Heart and Skin: Quantitation of MRI Visible Features
Provedor de dados: 17
Autores: S Sharma; Rakesh Sharma.
Excised mice kidney, heart and skin MRI visible features were quantitated using ex vivo 21 Tesla MRI microimager. The fast
MRI acquisition protocols of FLASH, gradient echo flow compensated (GEFC) techniques were used to visualize
microvasculature of mice kidney, mice heart including cardiac arteries, veins, cordate tendons and mice skin epidermis with hair
follicle features.
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3484/version/1
The anti-angiogenic herbal composition Ob-X inhibits adipose tissue growth in obese mice
Provedor de dados: 17
Autores: Min-Young Kim; Jong Cheon Hahm; Byung-Young Park; Hee Suk Lee; Eun kyu Park; Michung Yoon; Jinmi Lee;
Yeonhee Hong; Seolwha Choi; Hyunghee Lee.
Objective: The growth and development of adipose tissue are thought to be associated with angiogenesis and extracellular matrix
remodeling. Since the composition of the herbal extract called Ob-X has been shown to have both anti-angiogenic and matrix
metalloproteinase (MMP)-inhibiting activities, we hypothesized that growth of adipose tissue can be regulated by Ob-X. Materials
and Methods: The effects of Ob-X on angiogenesis and extracellular matrix remodeling were measured using in vitro and ex vivo
assays. The effects of Ob-X on adipose tissue growth were investigated with nutritionally obese mice. Results: Ob-X inhibited
angiogenesis in a dose-dependent manner in the human umbilical vein endothelial cell (HUVEC) tube formation assay in vitro and
the rat...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2970/version/1
Guided Tissue Remineralization of Resin-Bonded Acid-Etched Dentin
Provedor de dados: 17
Autores: Franklin R. Tay; David H. Pashley.
Contemporary biomineralization strategies incorporate non-classical crystallization pathways of fluidic amorphous nanoprecursors
and mesoscopic transformation. Using two functional biomimetic molecules, we previously regenerated mineralized dentin from
acid-etched dentin using the Guided Tissue Remineralization (GTR) approach, with definitive intrafibrillar remineralization of
type-I collagen. Degradation of denuded collagen within dentin adhesive resin-infiltrated dentin is a pertinent problem in dentin
bonding. Here, we show that GTR provides a means of salvaging these degrading bonds by remineralizing resin-dentin interfaces.
The GTR medium consists of a Portland cement/simulated body fluid that includes polyacrylic acid and polyvinylphosphonic
acid...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2022/version/1
Yeast surface 2-hybrid to detect protein-protein interactions via the secretory pathway as a platform
for antibody discovery
Provedor de dados: 17
Autores: Xuebo Hu; Sungkwon Kang; Xiaoyue Chen; Charles B. Shoemaker; Moonsoo M. Jin.
High throughput methods to measure protein-protein interactions will facilitate uncovering pairs of unknown interactions as well
as designing new interactions. We have developed a platform to detect protein interactions on the surface of yeast, where one
protein (bait) is covalently anchored to the cell wall and the other (prey) is expressed in secretory form. The prey is released either
outside of the cells or remains on the cell surface by its binding to the bait. The strength of their interaction is measured by
antibody binding to the epitope tag fused to the prey or direct readout of split fluorescence protein complementation. Our novel
'yeast surface 2-hybrid' system was found to differentiate 6-log difference in binding affinities...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2067/version/1
Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique
Provedor de dados: 17
Autores: Xiaohong Wang; Huirong Xu; Yongnian Yan; Renji Zhang.
Organ manufacturing products hold the promise to be used widely in the future for complex organ failings. The cryopreservation
of the product is a very important step in the commercialization activities. In this article, a new cell cryopreservation technique,
whereby, cryoprotectants were directly incorporated into the cell/hydrogel constructs, prototyped according to the predesigned
structure and then subjected to a special freezing/thawing process. The rheological and hydration properties of the
cryopreservation systems indicated that the hydratabilities of the gelatin/alginate hydrogels were greatly increased while the
eutectic temperatures were greatly decreased by the addition of glycerol. Dextran-40 was found to be effective to improve the
cell...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3722/version/1
Allele-specific primer based identification of dimeric alpha-amylase inhibitor
Provedor de dados: 17
Autores: Pradeep Sharma; Pooja Sharma; Manoj Saini; S. S. Singh.
Wheat is one of the most important staple food crops cultivated over 200 mha in the range of environment throughout the world.
Wheat production must continue to increase by 2% annually, more particularly in developing world including south-east Asia.
Besides increasing the inherent productivity of wheat, it is important to minimize the losses caused to production by various
abiotic and biotic factors. Alpha–amylase inhibitors are attractive candidates for the control of seed weevils as these
insects are highly dependent on starch as the energy source. They play an important role in the carbohydrate metabolism of many
heterotrophic and autotrophic organisms. In this study, we aimed to make sequence comparison and phylogenetic relationship
among...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5596/version/1
21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound
Nanoparticles
Provedor de dados: 17
Autores: Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH. After injecting
SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart
including cardiac arteries, veins, cordate tendons attached with valves.
The measurement accuracy of ventricles, aorta,
vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2643/version/1
Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their
reprogramming in mouse chimeras
Provedor de dados: 17
Autores: Bing Huang; KaiJing Li; Jie Yu; Min Zhang; YongPing Li; XiaoPing Xu; Ting Luo; JianFa Huang; LiPing Guan;
WeiHua Li; WenXin Zhang; ShaoChun Lin; XinTao Huang; LiPing Lin; YongLiang Lin; YiChi Zhang; WenCong Wang;
ZhiChong Wang; QianYing Gao; XiGu Chen; XinMing Song; Jian Ge.
Stem cells can be derived from the embryo (embryonic stem cells, ESCs), from adult tissues (adult stem cells, ASCs), and by
induction of fibroblasts (induced pluripotent stem cells, iPSs). Ethical problems, immunological rejection, and difficulties in
obtaining human tissues limit the use of ESCs in clinical medicine. Induced pluripotent stem cells are difficult to maintain in vitro
and carry a greater risk of tumor formation. Furthermore, the complexity of maintenance and propagation is especially difficult in
the clinic. Adult stem cells can be isolated from several adult tissues and present the possibility of self-transplantation for the
clinical treatment of a variety of human diseases. Recently, several ASCs have been successfully isolated and...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6016/version/1
Role of the porous structure of the bioceramic scaffolds in bone tissue engineering
Provedor de dados: 17
Autores: Wang Zhen; Chang Jiang; Bai Feng; Sun Xiaojiang; Lu Jianxi; Lin Li; Lei Chen; Dai Rong.
The porous structure of biomaterials plays a critical role in improving the efficiency of biomaterials in tissue engineering. Here we
fabricate successfully porous bioceramics with accurately controlled pore parameters, and investigate the effect of pore parameters
on the mechanical property, the cell seeding proliferation and the vascularization of the scaffolds. This study shows that the
porosity play an important role on the mechanical property of the scaffolds, which is affected not only by the macropores size, but
also by the interconnections of the scaffolds. Larger pores are beneficial for cell growth in scaffolds. In contrast, the
interconnections do not affect cell growth much. The interconnections appear to limit the number of blood vessels...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4148/version/1
Low Cardiorespiratory Fitness is an Independent Predictor of Metabolic Syndrome in Young Korean
Adults
Provedor de dados: 17
Autores: Hyun-Sik Kang; Soo-Hyun Park; Shinuk Kim.
Objective: To investigate the relationship between cardio/respiratory fitness (CRF) and metabolic syndrome (MS) in young
Korean men. Design: In a cross-sectional study, we examined 909 young Korean men (mean±SD age,
24.0±2 years) who were healthy and not taking any medications affecting blood pressure, glucose, or lipids
concentrations. Body fatness, resting blood pressures, and fasting blood levels of lipids, glucose, and insulin were measured with
our standardized laboratory protocols. CRF was quantified as the maximum volume of minute oxygen consumption measured
during a graded treadmill test. Metabolic syndrome was defined with the National Cholesterol Education Program Adult
Treatment Panel III criteria and a modified cut-off value...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2236/version/1
Clean Coal and Spirulina maxima (Cyanobacteria) Production through Alkaline-Seawater
Provedor de dados: 17
Autores: Md. Abu Affan; Dae-Won Lee; Heung-Sik Park; Jae-Hoon Noh; Soo-Jin Heo; Chulhong Oh; Seon-Mi Jeon; Joo-Won
Son; Seung-Gyu Son; Hyi-Seung Lee; Hyeon Yong Lee; Do-Hyung Kang.
Carbon capture and storage have involved high cost and an earthquake threat. Coal in NaOH-natural seawater (NSW) can produce
clean coal, and organic carbon enriched pretreated sweater (PSWS) can be used to grow Spirulina maxima. However, with
freshwater shortage, high cost CO2 feedstock incurs S. maxima biomass production, and using PSWS should be considered as an
alternative. NaHCO3 and Na2CO3 were used to sediment Mg2+ and Ca2+ of NSW to resolve turbidity and decreasing phosphate
solubility. Here, we show that pre-combustion coal and NaOH concentration calculated by our devised formulae for any salinity
could dilute volatile matter 19.8 Kg plus CO2 116.50 to 168.89 Kg Ton^-1^ coal in NaOH-NSW by sedimentation Mg2+ and
Ca2+ of 99.91% and 32.52%,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6930/version/1
Rapid antigen detection using the liquid sample as a lens and self-mixer for light scattering detection
Provedor de dados: 17
Autores: Antonio Garcia; John Schneider.
Superhydrophobic surfaces, both natural and artificial, cause aqueous drops to bead on them. The most water-repellent surfaces
produce nearly perfect spheres with very little contact between water, solutes, and surface due to the roughness of the surface ^1^.
However, a related phenomenon that has not been well appreciated until recently, is that colloidal particles in the aqueous drop
remain highly mobile, and smaller-sized colloids migrate to the drop surface due to both the driving force of evaporation and the
expanded liquid/air interface ^2-3^. A newly identified, third useful optical property resulting from the suspension of an aqueous
drop by the superhydrophobic surface is that a spheroidal lens is created. Without any additional energy or...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4754/version/1
Ultrasound-targeted microbubble destruction enhances AAV mediated gene transfection: human
RPE cells in vitro and the rat retina in vivo
Provedor de dados: 17
Autores: Hongli Li; Xiaozhi Zheng; Huiping Wang; Fan Li; Ying Wu; Lianfang Du.
The present study was performed to investigate the efficacy and safety of Ultrasound-targeted microbubble destruction (UTMD)
mediated rAAV2-EGFP to cultured human retinal pigment epithelium (RPE) cells _in vitro_ and the rat retina _in vivo_. _In
vitro_ study, cultured human RPE cells were exposed to US under different conditions with or without microbubbles.
Furthermore, the effect of UTMD to rAAV2-EGFP itself and the cells were evaluated. _In vivo_ study, gene transfer was
examined by injecting rAAV2-EGFP into the subretinal space of the rats with or without microbubbles and then exposed to US.
We investigated EGFP expression _in vivo_ via stereomicroscopy and performed quantitative analysis by Axiovision 3.1
software. HE staining and frozen sections...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2898/version/1
Trichoscopy criteria for diagnosing female androgenic alopecia.
Provedor de dados: 17
Autores: Adriana Rakowska; Monika Slowinska; Elzbieta Kowalska-Oledzka; Malgorzata Olszewska; Lidia Rudnicka.
Differential diagnosis of chronic hair loss remains a challenge in dermatology. The aim of the study was to evaluate the value of a
new scalp visualization technique, trichoscopy, in differential diagnosis of hair loss. Trichoscopy was performed in 131 females
(59 with androgenic alopecia, 33 with chronic telogen effluvium, 39 healthy controls). Based study results, a standardized
trichoscopy report was developed and diagnostic criteria for female androgenic alopecia were established. Major criteria:
increased number of yellow dots and thin hairs, as well as decreased average hair thickness in frontal area. Minor criteria:
increased frontal area to occiput ratio of single-hair units (>2:1), vellus hairs (>1.5:1) and follicles with...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1913/version/1
21 Tesla Micro-MRI of Rat Skin
Provedor de dados: 17
Autores: Rakesh Sharma; Suniket Fulzele; Kiran Shetty; Mandeep Sachdeva; Bruce R. Locke.
*Aim:* A rapid ultra high resolution ex vivo MRI imaging method was evaluated to visualize rat skin structure. The main
objectives were:1.qualitative assessment of viable epidermis, hair, oil and fat-rich skin features by multi-contrast
approach;2.achievement of spatial resolution up to 15 microns; 3.comparison of skin histology with MRI skin features obtained by
21 Tesla magnetic resonance imaging (MRI) imager. 

*Introduction:* The ultrawide
bore 900 MHz magnet has high resolution NMR magnet has a central field of 21.1 T. The high resolution MRI technique has
potential for both quantitative and non-invasive morphological evaluation at micrometer level. The MRI signal was sensitive to
water protons and glycolipid...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2639/version/1
Optimising Blue Fluorescent Protein (BFP) for use as a mammalian reporter gene in parallel with
Green Fluorescent Protein (GFP).
Provedor de dados: 17
Autores: Etienne Joly.
To obtain an optimised form of BFP for use as a reporter gene in mammalian cells, the brightest available GFP form, EGFP, was
mutated at 5 different positions, yielding 8 different mutagenised forms of BFP. The intensity of the fluorescent signals attained in
mammalian cells with all these various versions of BFP was analysed by flow cytometry of transiently transfected COS 7 cells.
The best mutant obtained can be detected readily both by flow cytometry and fluorescence microscopy, even when expressed
together with GFP. To explore whether cellular localization could enhance the fluorescence signals any further, plasmid constructs
were made to target optimised versions of GFP and BFP to the nucleus, the endoplasmic reticulum (ER) and the cell surface....
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1259/version/1
Inhibition of pteridine reductase 1 (PTR1) expression in Leishmania promastigotes using a full-length
antisense construct
Provedor de dados: 17
Autores: Farnaz Kheirandish; Mojgan Bandehpour; Nushin Davoudi; Nariman Mosaffa; Fereydoun Mahboudi; Bahram Kazemi;
Ali Haghighi; Mehdi Mohebali.
Leishmania exhibit many unusual features, one of which is the pteridine metabolic pathway essential for the growth for these
parasites. Leishmania have evolved a complex and versatile pteridine salvage network capable of scavenging a wide array of
conjugated and unconjugated pteridines. The L. major PTR1 gene was cloned into pcDNA3 digested with KpnI and BamHI. The
gene was cloned antiparallel to the promoter and named pcDNA-Rptr. L. major promastigotes were divided into two groups. One
group was transfected with 50 μg of pcDNA-Rptr, whereas the other group was electroporated with pcDNA3.
Mentioned cells were cultured and plated onto semi-solid media.Western blotting was performed on extracts from transfected
promastigotes of L. major using an...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2807/version/1
Intelligence-based medicine
Provedor de dados: 17
Autores: Pierre Squara; Didier Journois.
Despite seven hundred thousand new medical references last year, the relationship between a given set of medical features and
specific pathophysiology, treatment, and criteria of improvement is often weak. Moreover, the generalization of evidences
obtained in specific settings may lead to under-treat or to over-treat a significant proportion of patients. We expose an application
of the cybernetic loop, based on traditional medical steps: nosology, semeiology, pathophysiology, therapy and on the four
transitions between these steps. This approach leads to formulate eight basic questions evaluating the steps in terms of
reproducibility and the transitions in terms of predictivity. We detail two practical applications: 1) the evaluation of a medical
decision...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3196/version/1
Phagocytic Activity and Hexose Monophosphate Shunt Activity of Cultured Human Kupffer Cells
upon Zymosan, Erythrocytes, Amoebic Trophozoits, Latex Beads and Nitroimidazole
Provedor de dados: 17
Autores: Rakesh Sharma; Vijay S. Singh.
Phagocytic function of human Kupffer cells was altered by the addition of nitroimidazole and amoebic trophozoits. The
hypothesis was that phagocytic activity was dependent on the nature of additives in culture. The Kupffer cells were fractionated
and lysosomes were isolated for enzyme estimations in presence of additives. Phagocytic activity function of human Kupffer cells
was altered by the addition of nitroimidazole and amoebic trophozoits in Kupffer cell culture. The human Kupffer cells showed
take up of zymosan particles larger than 1 micron in diameter and inert interaction with latex particles. The liver endothelial cells
or phagocytes constitute a second line of defense in the liver for the phagoctosis function. The nitroimidazole slowed down the...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4300/version/1
A novel class of endogenous shRNAs in human cells
Provedor de dados: 17
Autores: Tongjun Gu; James Q. Yin; Yanwei Xu; ZhengHua Dai; Zhengge Qiu; Shenzhong Feng; Xiang Yi; Ling Jiang; Hongjie
Zhang.
Until now it is still not clear how many types and amounts of small RNAs (sRNAs) exist in humans. Here we report the
identification of 1258 distinct sRNAs derived from intronic regions of protein-coding genes in human with a new approach. These
endogenous short hairpin RNAs (shRNAs) appear to be similar to exogenous shRNAs in structure, have a broad distribution in the
stem length, and function as microRNAs (miRNAs), small interfering RNAs (siRNA) and/or piwi-interacting RNAs (piRNAs).
Except for a few shRNAs, the majority of shRNAs are not phylogenetically conserved. They are differentially expressed in
different cells and at diverse developmental stages. Overall, their expression levels are lower than miRNAs', but can
be detected by quantitative...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1531/version/1
Regulating Nanomedicine: A European perspective
Provedor de dados: 17
Autores: Joel D'Silva; Geert Van Calster.
The potential impact of nanomedicine is foreseen to radically change health care; however it also challenges existing perceptions,
dynamics and standards relating to ethics, safety and governance. This commentary briefly highlights aspects and approaches
pertaining to nanomedical regulation in the European Union.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2388/version/1
Cell Proteomic Footprint
Provedor de dados: 17
Autores: Petr Lokhov; Elena Balashova; Maxim Dashtiev.
The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell
therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and
straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low
sample
cost,
and
high
sensitivity.
We
discovered
that
cell
cultures
treated
with
protease
at
soft,
“non-killing” conditions release fragments of cell surface proteins, which composition is a strong
characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass
spectrum with the mass spectrum of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2091/version/1
Consortium Building For PEM MFC Using Synthetic Media As Substrate
Provedor de dados: 17
Autores: Muralidharan Ayyappan; Dimpal Parida; Arti Yadav; Ramya Mohandass.
Microbial production of electricity is an important form of bioenergy since Microbial Fuel cells (MFC) offer the possibility of
extracting electric current from a wide range of organic wastes and renewable biomass. Factors affecting the MFC operational
effectiveness are the MFC design and the bacterial metabolism and electron transfer. The purpose of this study is to identify
species which are responsible for electricity generation so as to build a suitable consortium and to investigate the relative
efficiencies between the microbial consortiums. Enrichment by repeated transfer of a bacterial consortium harvested from the
anode compartment of a MFC with synthetic media as a substrate increased the output from an initial level of 34 mA to a maximal
level of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6632/version/1
Cell-free Embryonic Stem Cell Extract-mediated Derivation of Multi-potent Stem Cells from NIH3T3
Fibroblasts for Functional and Anatomical Ischemic Tissue Repair
Provedor de dados: 17
Autores: Johnson Rajasingh; Hiromichi Hamada; Evelyn Bord; Tina Thorne; Ilona Goukassian; Erin Lambers; Prasanna
Krishnamurthy; Gangjian Qin; Karen Schlauch; Kenneth M. Rosen; Deepali Ahluwalia; Yan Zhu; Douglas W. Losordo; Raj
Kishore.
The oocyte-independent generation of multipotent stem cells is one of the goals in regenerative medicine. We report that upon
exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by
stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant
differences between NIH3T3 and ESC-extract treated NIH3T3 cells including re-activation of ESC specific transcripts.
Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased
lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial
infarction (AMI)...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1208/version/1
Biological robotics and nanorobotred cells: Characterization and applications
Provedor de dados: 17
Autores: Rakesh Sharma; Soonjo Kwon; Ching J. Chen.
In human body, nanorobot-like red blood cells get exposed to human body fluids and many physico-chemical changes inside
while these nanorobots (fullerene structure coated hemoglobin embedded iron inorganic elements) remain safe and perform killer
action against bacteria or virus. We propose this killer action as ‘nanorobot driven nanomedical
treatment’ to combat a bacterial or viral infection. The red cells also act as imaging agent due to oxygen binding
character. Our experiments were focused on the application of nanorobot (red cells) to make them immune resistant against
viruses and making them as useful contrast agents in magnetic resonance imaging use based on their behavior in nanomagnetic
fields. It consists of an injection of...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4262/version/1
Oilseed Rape straw for Cultivation of Oyster Mushroom
Provedor de dados: 17
Autores: Gholamali Peyvast; Ahmad Nowroozi; Jamal-Ali Olfati.
Oyster mushroom [_Pleurotus ostreatus_ var. _sajor caju_ (Fr.) Singer] cultivation can play an important role in managing organic
waste. It can be cultivated on a wide rang of substrates containing lignin and cellulose. Oyster mushroom was grown on five
substrates: Rise straw (Oryza sativa L. var. Alikazemi), Rise straw + Oilseed Rape straw (Brassica napus var. Hyola 401) (75:25
dw /dw), Rise straw + Oilseed Rape straw (50:50 dw /dw), Rise straw + Oilseed Rape straw (25:75 dw /dw) and Oilseed Rape
straw alone. Oilseed Rape straw alone and Rise straw + Oilseed Rape straw (25:75 dw /dw) were best for fruit body production of
P. ostreatus. Time to fruiting for P. ostreatus was also shorter on Oilseed Rape straw. Protein content of the fruit bodies obtained...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2069/version/1
Different Approaches For Protein Engineering In Industrial Biotechnology
Provedor de dados: 17
Autores: Israr Khan; Muhammad Waheed Akhtar.
Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and
research. It may apply rational design or non rational design or both. Site directed mutagenesis is a classical approach involving
the protein folding principles and as such different techniques involving multidisciplinary research and broad knowledge is
required involving biocomputing of complex data obtained from various sequencing projects and prediction of the future protein
structure either chemically or genetically modified. Non rational mutagenesis or directed evolution involves random mutations in
the gene encoding protein or shuffling the genes encoding different domains producing a random set of numerous large libraries
of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5601/version/1
Local and global modes of drug action in biochemical networks
Provedor de dados: 17
Autores: Jean-Marc Schwartz; Jose C. Nacher.
It becomes increasingly accepted that a shift is needed from the traditional target-based approach of drug development to an
integrated perspective of drug action in biochemical systems. We here present an integrative analysis of the interactions between
drugs and metabolism based on the concept of drug scope. The drug scope represents the set of metabolic compounds and
reactions that are potentially affected by a drug. We constructed and analyzed the scopes of all US approved drugs having
metabolic targets. Our analysis shows that the distribution of drug scopes is highly uneven, and that drugs can be classified into
several categories based on their scopes. Some of them have small scopes corresponding to localized action, while others have
large scopes...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2516/version/1
Comparison of Plasmid yield between L.B. and using Molasses as a medium
Provedor de dados: 17
Autores: Nitin Sharma; Purshottum Kaushik.
During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate
the C/N ratio as per requirement of cell. Molasses is one of the best alternatives as it is cheap and can be easily manipulated.
Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals. it is possible to produce
vaccine by molasses medium. The paper is opening a new face of study.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3897/version/1
Composite genetic structure of rice land races revealed by STMS markers
Provedor de dados: 28
Autores: Kohli, Sudha; Mohapatra, T; Das, S R; Singh, A K; Tandon, V; Sharma, R P.
Land races adapted to a variety of agro-ecological niches constitute a rich source of rice biodiversity. Large-scale cultivation of
high-yielding varieties has resulted in the replacement of the land races, except a few that are still being cultivated by the
resource-poor farmers practising subsistence farming. From the viewpoints of their conservation and utilization, it is important to
understand the genetic structure of the present-day land races. The present study was desig- ned to explore if the present-day land
races of rice still retain a composite genetic structure that forms the basis of their stability. Two land races, which are being grown
by the rural farmers in the coastal regions of Orissa over several generations, were characterized and...
Tipo: Article
Palavras-chave: Biotechnology; Genetics.
Ano: 2004
URL: http://eprints.iari.res.in/133/1/850.pdf
21 Tesla Rat Heart Magnetic Resonance Microimaging by Paramagnetic Anti-Troponin Bound
Polyethylene Based Iron-Oxide Nanoparticles and Image Processing
Provedor de dados: 17
Autores: Rakesh Sharma.
21T MR microimaging with the first use of troponin nanoparticles enhances the visualization of cardiac muscle fibers and offers
technical advancement for the future. Diffusion weighting offers fiber tracking and functional analysis. Image processing offers
heart
probabilistic
atlas
and
maps.

http://www.ismrm.org/10/program/ELECTRONICPOSTERS.htm
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6650/version/1
Recombinant Mitochondrial Transcription Factor A with N-terminal Mitochondrial Transduction
Domain Increases Respiration and Mitochondrial Gene Expression in G11778A Leber's
Hereditary Optic Neuropathy Cybrid Cells
Provedor de dados: 17
Autores: Shilpa Iyer; Ravindar R. Thomas; Francisco R. Portell; Lisa D. Dunahm; Caitlin K. Quigley; James P. Bennett.
Diseases involving mitochondrial defects usually manifest themselves in high-energy, post-mitotic tissues such as brain, retina,
skeletal and cardiac muscle and frequently cause deficiencies in mitochondrial bioenergetics. We have developed a scalable
procedure to produce recombinant human mitochondrial transcription factor A (TFAM) modified with an N-terminal protein
transduction domain (PTD) and mitochondrial localization signal (MLS) that allow it to cross membranes and enter mitochondria
through its "mitochondrial transduction domain" (MTD,=PTD+MLS). _In vitro_ studies in a classic
mitochondrial disease cell model demonstrated that Alexa488-labeled MTD-TFAM rapidly entered the mitochondrial
compartment. MTD-TFAM treatment of these...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2084/version/1
The impact of Bt cotton on poor households in rural India
Provedor de dados: 17
Autores: Arjunan Subramanian; Matin Qaim.
The impact of genetically modified (GM) crops on the poor in developing countries is still the subject of controversy. While
previous studies have examined direct productivity effects of _Bacillus thuringiensis_ (Bt) cotton and other GM crops, little is
known about wider socioeconomic outcomes. We develop a microeconomic modeling approach and use comprehensive survey
data from India to analyze welfare and distribution effects in a typical village economy. Bt cotton adoption increases aggregate
employment with interesting gender implications. Likewise, aggregate household incomes rise, including for poor and vulnerable
farmers, suggesting that Bt cotton contributes to poverty reduction and rural development.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2008
URL: http://precedings.nature.com/documents/1812/version/1
Systems Biology Graphical Notation: Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas Le Novere; Emek Demir; Huaiyu Mi; Falk Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without
electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialized
notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields.
With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions
of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few
detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/3721/version/3
Donor mesenchymal stem cells trigger chronic graft-versus-host disease following minor
antigen-mismatched bone marrow transplantation
Provedor de dados: 17
Autores: Yoko Ogawa; Satoru Morikawa; Hideyuki Okano; Yo Mabuchi; Sadafumi Suzuki; Tomonori Yaguchi; Saori Yaguchi;
Takaaki Inaba; Shinichiro Okamoto; Yutaka Kawakami; Kazuo Tsubota; Shigeto Shimmura; Yumi Matsuzaki.
Chronic graft-versus-host disease (cGVHD) is a complication after minor antigen mismatched bone marrow transplantation
(BMT) characterized by an autoimmune-type reaction in various organs. Aberration in T cell regulation is involved, with donor
mesenchymal stem cells (MSCs) playing a possible role in immunomodulation. In a minor-antigen mismatched mouse BMT
model, transplantation of mismatched, but not syngeneic MSCs triggered the onset of cGVHD, and was associated with fibrosis,
increased IL-6 secretion, decreased Foxp3+ regulatory T cells and increased Th17 in the peripheral blood. Mismatched MSCs
alone were sufficient to induce cGVHD, while removal of donor MSCs rescued mice from cGVHD. RAG2 knockout recipient
mice did not suffer cGVHD, indicating that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology.
Ano: 2012
URL: http://precedings.nature.com/documents/6843/version/1
SBGN-ED - a tool for editing, validating, and translating of SBGN maps
Provedor de dados: 17
Autores: Tobias Czauderna; Christian Klukas; Astrid Junker; Falk Schreiber.
The recently proposed Systems Biology Graphical Notation (SBGN) provides a standard for the visual representation of
biochemical and cellular processes. SBGN helps to communicate biological knowledge more efficient and accurate between
different research communities in the life sciences. However, to support SBGN, methods and tools for editing, validating, and
translating of SBGN maps are necessary.

We present methods for these tasks, the tool
SBGN-ED, and an application example. SBGN-ED allows to create all three types of SBGN maps from scratch, to validate these
maps for syntactical and semantical correctness, to translate maps from the KEGG and MetaCrop databases into SBGN, and to
export SBGN maps into several file and...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4973/version/1
Enzyme activity and dynamics in near-anhydrous conditions
Provedor de dados: 17
Autores: Murielle Lopez; Vandana Kurkal-Siebert; Rachel Dunn; Moeava Tehei; John Finney; Jeremy Smith; Roy Daniel.
Water is widely assumed to be essential for life 1, although the exact molecular basis of this requirement is unclear 2-4. Water
facilitates protein motions 5-9 and although enzyme activity has been demonstrated at low hydrations in organic solvents 10-13,
such non-aqueous solvents may allow the necessary motions for catalysis. To examine enzyme function in the absence of
solvation and bypass diffusional constraints we have tested the ability of an esterase to catalyse alcoholysis as an anhydrous
powder, using a closed reaction system in which the substrates and products of the enzyme reaction are gaseous 14-15, and where
the water content can be well defined 16. At hydrations equivalent to 3 (±2) molecules of water per molecule of
enzyme,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3884/version/1
Arbuscular mycorrhizal fungus spores host bacteria and their biofilm efficient in nutrient
biodynamics and soil-borne plant pathogen suppression
Provedor de dados: 17
Autores: Andre Cruz; Takaaki Ishii.
The combination of bacteria and arbuscular mycorrhizal fungi (AMF) would be ideal for nutrient biodvnamics in soils and
protection from soil-borne plant pathogens (SBPP). During the process of plant colonization AMF interact with bacteria where
spores cytoplasm and hvphae provide specific niches for certain populations of bacteria. The clarification of the relationship
AMF-bacteria has great potential for sustainable agriculture. The currently research aimed to isolate and characterize bacteria.
Which we have designated most probable endobacteria (MPE) from an AMF spore. Three bacterial strains were taken from about
500 spores by using a 200 µm diameter hvpodermic needle. These bacteria were identified by DNA and morphological
methods. Antagonism...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/5544/version/1
Peptides as potent antimicrobials tethered to a solid surface: Implications for medical devices
Provedor de dados: 17
Autores: Ivan A. Gonzalez; XiXi Wong; Dilhari DeAlmeida; Raymond B. Yurko; Simon C. Watkins; Kazi Islam; Ronald C.
Montelaro; Ahmed El-Ghannam; Timothy A. Mietzner.
Medical devices are an integral part of therapeutic management; despite their importance, they carry a significant risk of microbial
infection. Bacterial attachment to a medical device is established by a single, multiplying organism, leading to subsequent biofilm
formation. To date, no preventative measures have impacted the incidence of device-related infection. We report the bidirectional
covalent coupling of an engineered cationic antimicrobial peptide (eCAP), WLBU2, to various biological surfaces is
accomplished. These surfaces included (i) a carbohydrate-based gel matrix, (ii) a complex polymeric plastic bead, and (iii) a
silica-calcium phosphate nanocomposite associated with bone reconstruction. WLBU2-conjugated surfaces are shown to retain
potent...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/1967/version/1
Methanogen consortium metabolism characteristic analysis of Illinois basin by real time monitor
method
Provedor de dados: 17
Autores: Dong Xiao; Enyuan Wang.
Microbially Enhance Coalbed Methane technology is used to improve methane content in coalbeds. The methanogen consortium
metabolism process is the basic avenue for MECoM to yield methane with some organic compounds in coal. Detailed information
about the metabolism process is needed in studying the microbial consortium mechanism. Traditional gas analysis is limited by
gas source and test complexity. The real time monitor with Infrared gas sensor breaks the gas analysis limitation. It shows much
more detailed information than the traditional analysis method including information about microbial growth factor changes,
reaction speeds for environmental changes, the detailed relationship between CH4 and CO2, methane yield and the consumption
factor by real...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/5426/version/1
Exchanging Experimental Kinetic Data via SabioML
Provedor de dados: 17
Autores: Martin Golebiewski.
The simulation of quantitative biochemical models not only requires qualitative information about the stoichiometry of the
described networks, but also kinetic data describing their dynamics. Such kinetic data have to be experimentally measured,
collected, systematically structured and stored to finally make them accessible. However, the dataflow from the experiment to the
model is still a bottleneck, calling for systems that capture the data directly from the instrument, process and normalize it to
agreed
standards
and
finally
transfer
the
data
to
publicly
available
databases.

SABIO-RK (http://sabio.h-its.org) is a curated database system which we
have developed for bundling data referring to biochemical reactions and...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5030/version/1
Nanofiber fabrication in a temperature and humidity controlled environment for improved fibre
consistency
Provedor de dados: 17
Autores: Oliver Hardick; Bob Stevens; Daniel G. Bracewell.
To fabricate nanofibers with reproducible characteristics, an important demand for many applications, the effect of controlled
atmospheric conditions on resulting electrospun cellulose acetate (CA) nanofibers was evaluated for temperature ranging 17.5 35°C and relative humidity ranging 20% - 70%. With the potential application of nanofibers in many industries,
especially membrane and filter fabrication, their reproducible production must be established to ensure commercially
viability.
Cellulose acetate (CA) solution (0.2 g/ml) in a solvent mixture of acetone/DMF/ethanol (2:2:1)
was
electrospun
into
nonwoven
fibre
mesh
with
the
1µm.
The resulting nanofibers were...
fibre
diameter
ranging
from
150nm
to
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4524/version/1
Hybrid Extraction Method; Innovative Method to Produce Non Oxidative Tuna Oil
Provedor de dados: 17
Autores: Yoshinori Ito.
Summary: The function of Polyunsaturated Fatty Acid such as DHA, EPA are now well known world widely through strong
scientific evidences and the importance of nutritional value and popularity are increasing every day all over the world with the
name of omega 3 fatty acids.^1,2,3^ However, the fish oil, the source of omega 3 fatty acid had been considered as very
vulnerable to oxidation. Many papers say the reason why is because its very easy to oxidize fish oil. The greater the
Polyunsaturated Fatty Acid contents in the fish oil is, the faster oxidation takes place.^4,5^ Here I show that tuna oil extracted
from Albacore Tuna Head in closed circuit under low temperature, at first decompressing then pressurizing and heating in a
container, is very stable...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2009
URL: http://precedings.nature.com/documents/3110/version/1
Ontology for Biomedical Investigations
Provedor de dados: 17
Autores: Bjoern Peters; The OBI Consortium.
The goal of OBI is to enable a formal representation of biomedical investigations that captures the experimental evidence on
which their findings are based. The scope of OBI includes: materials made in and produced for investigations, research objectives,
experimental protocols, roles of people in investigations and processing and publication of data gathered in investigations. Use of
OBI will allow comparison of experimental data from the wide array of scientific disciplines represented by domain experts in the
OBI consortium. OBI follows the principles laid out by the OBO foundry, and integrates tightly with other foundry candidate
ontologies, such as GO (www.geneontology.org) and ChEBI (www.ebi.ac.uk/chebi/) whose terms are used to describe
biological...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3623/version/1
Apply of Textmining Method to Study the Roles in Improving the Health by Lactoferrin, a
Multi-Functional Milk Protein
Provedor de dados: 17
Autores: Kei-ichi K. Shimazaki; Tatsuya T. Kushida; Koji K. Yamauchi; Toshihisa T. Takagi.
Lactoferrin is a metal-binding glycoprotein found in milk, blood and other exocrine secretions. This is a multi-functional protein
that exhibits many activities such as: anti-microbial, anti-viral, immunomodulatory, anti-inflammatory, anti-tumor,
anti-metastatic, cell growth-promoting, and anti-oxidant activities, as well as regulation of granulopoiesis and iron absorption, etc.
To date, a number of academic reports concerning the biological activities of lactoferrin have been published and are easily
accessible through public databases. In order to overcome the information overload associated with lactoferrin information, we
have applied the text mining method to the accumulated lactoferrin literature. To this end, we used the information extraction...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/5100/version/1
ECoG-based short-range recurrent stimulation techniques to stabilize tissue at risk of progressive
damage: Theory based on clinical observations
Provedor de dados: 17
Autores: Markus A. Dahlem; Felix M. Schneider; Jens P. Dreier; Anthony J. Strong; Eckehard Schoell.
We introduce theoretical concepts based on chaos control to stabilize in acute stroke the tissue at risk of progressive damage by
preventing adverse effects of waves of mass neuronal depolarization. Moreover, we present clinical electrocorticography (ECoG)
recordings of relevant signals suggested for the feedback control. The recordings are performed in combination with novel
subdural opto-electrode technology for simultaneous laser-Doppler flowmetry in patients with aneurysmal subarachnoid
haemorrhage (aSAH). In aSAH patients waves of spreading depolarization (SD) have a high incidence and cause hypoxia in tissue
at risk, and, importantly, the haemodynamic response is the inverse of that seen in healthy tissue. In previous clinical studies,
clusters of...
Tipo: Poster
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3408/version/1
Topological Characterization of Protein-Protein Interaction Networks in Human and Mouse
Provedor de dados: 17
Autores: Bipin Singh.
The elucidation of the cell's large-scale organization is a primary challenge for post-genomic biology, and
understanding the structure and topological properties of protein-protein interaction networks offers an important starting point for
such studies. We compare the protein-protein interaction network of the human and mouse, aiming to uncover the
network's generic large-scale properties and the impact of the protein's function and cellular localization
on the network topology. We show that both human and mouse protein-protein interaction supports a scale-free, topology with
hierarchical modularity, indicating that these features represent a robust and generic property of the protein interactions network.
We also find strong...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6126/version/1
DNALinux Virtual Desktop Edition
Provedor de dados: 17
Autores: Sebastian Bassi; Virginia V. C. Gonzalez.
The new version of DNALinux (VDE) is presented. DNALinux VDE is a departure from traditional distributions since it uses a
virtual machine to bundle together the operating system and bioinformatics applications. The main advantage of this approach is
that a virtualized environment doesn't affect a installed system. With a virtual machine a Linux system can be run
under a Windows system, provided that the virtual machine player is installed. The included programs are listed and specifications
to add more programs are explained. We believe that DNALinux could be used as a standardized virtual machine for learning,
using, developing and testing bioinformatics applications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/670/version/1
Laparoscopy Pneumoperitoneum Fuzzy Modeling
Provedor de dados: 17
Autores: Otavio M. Becker Jr.; Jose E. Araujo Jr.; Joao L. M. C. Azevedo.
Abstract: Gas volume to intra-peritoneal pressure fuzzy modeling for evaluating pneumoperitoneum in videolaparoscopic surgery
is proposed in this paper. The proposed approach innovates in using fuzzy logic and fuzzy set theory for evaluating the accuracy
of the prognosis value in order to minimize or avoid iatrogenic injuries due to the blind needle puncture. In so doing, it
demonstrates the feasibility of fuzzy analysis to contribute to medicine and health care. Fuzzy systems is employed here in
synergy with artificial neural network based on backpropaga tion, multilayer perceptron architecture for building up numerical
functions. Experimental data employed for analysis were collected in the accomplishment of the pneumoperitoneum in a random
population of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6060/version/1
Migraine aura: retracting particle-like waves in weakly susceptible cortex
Provedor de dados: 17
Autores: Markus A. Dahlem; Nouchine Hadjikhani.
Cortical spreading depression (SD) has been suggested to underlie migraine aura. Despite a precise match in speed, the
spatio-temporal patterns of SD and aura symptoms on the cortical surface ordinarily differ in aspects of size and shape. We show
that this mismatch is reconciled by utilizing that both pattern types bifurcate from an instability point of generic reaction-diffusion
models. To classify these spatio-temporal pattern we suggest a susceptibility scale having the value [sigma]=1 at the instability
point. We predict that human cortex is only weakly susceptible to SD ([sigma]<1), and support this prediction by
directly matching visual aura symptoms with anatomical landmarks using fMRI retinotopic mapping. We discuss the increased...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2070/version/1
Tendril, adhesive disc and super adhesive effect of climbing plant
Provedor de dados: 17
Autores: Wenli Deng.
Understanding the super adhesion mechanism of biological system is of great scientific interest and a prerequisite for bioinspired
design of adhesive systems. To investigate the versatile climbing plant requires the development of new methods. Here I present
strategies to fully study the super adhesive effect of climbing plant _Parthenocissus tricuspidata_ by the first time measuring the
mass and the attached area of single adhesive disc, by further determining the microscopic structure and the adhesive strength of
single adhesive disc and by finally elucidating the adhesion mechanism in cellular and molecular level using the classical theories
and the proposed new hypothesis and model. I have measured that a single mature adhesive disc has an average mass...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1513/version/2
SSR - Identification from EST
Provedor de dados: 17
Autores: Aikkal Riju.
Tutorial of Identification of SSR from EST resources using online Tools
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3908/version/1
Photoelectrochemical properties of melanin
Provedor de dados: 17
Autores: Arturo Solis; Maria E. Lara; Luis E. Rendon.
Melanin is to the animal kingdom like chlorophyll to the vegetal kingdom(1). Melanin collects energy from lower-energy
radiation sources, kicks electrons into excited states, initiating a process that would end up producing chemical energy, similar to
the way in which photosynthesis supplies energy to plants. However, the precise roles of melanin during this process are
unknown. Here we show that the increase in the electron-transfer properties of melanin is independent of the energy of the
incident photons. We found in controlled in vivo assays that melanin has the remarkable capability of converting lower-energy
radiation towards a more useful form of energy. Furthermore, we found that melanin can break up water molecules and giving up
energy suggesting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1312/version/1
Simcluster: clustering enumeration gene expression data on the simplex space
Provedor de dados: 17
Autores: Ricardo Z. N. Vêncio; Leonardo A. B. Varuzza; Carlos Pereira; Helena Brentani; Ilya Shmulevich.
Transcript
enumeration
methods
such
as
SAGE,
MPSS,
and
sequencing-by-synthesis
EST
"digital
northern", are important high-throughput techniques for digital gene expression measurement. As other counting or
voting processes, these measurements constitute compositional data exhibiting properties particular to the simplex space where the
summation of the components is constrained. These properties are not present on regular Euclidean spaces, on which
hybridization-based microarray data is often modeled. Therefore, pattern recognition methods commonly used for microarray data
analysis may be non-informative for the data generated by transcript enumeration techniques since they ignore certain
fundamental properties of this...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/202/version/1
Overnight weight loss: relationship with sleep structure and heart rate variability
Provedor de dados: 17
Autores: Walter Moraes; Dalva Poyares; Christian Guilleminault; Agostinho Rosa; Marco Tulio Mello; Adriana Rueda; Sergio
Tufik.
Background: Weight loss can be caused by a loss of body mass due to metabolism and by water loss as unsensible water loss,
sweating, or excretion in feces and urine. Although weight loss during sleep is a well-known phenomenon, it has not yet been
studied in relation to sleep structure or autonomic tonus during sleep. Our study is proposed to be a first step in assessing the
relationship between overnight weight loss, sleep structure, and HRV (heart rate variability) parameters.Methods: Twenty-five
healthy volunteers received a 487 kcal meal and 200 ml water before experiment. Volunteers were weighed before and after
polysomnography. Absolute and relative weight indices were calculated. Time and frequency domain analysis of heart rate
variability was...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2342/version/1
First reports of computed tomographic colonography for the screening of colorectal polyps in
acromegalic patients
Provedor de dados: 17
Autores: Odery Ramos Jr; César Luiz Boguszewski; Sandra Teixeira; Anke Boeving; Ricardo De Bem; Benito
Parolim; Júlio César Pisani; João Carlos Prolla.
*Aim:*
To
analyze
the
CTC
performance
for
the
screening
of
colorectal
polyps
in
acromegalic
patients.


*Materials and Methods:* A prospective study of 21 acromegalic patients, 12 male
and 9 female, average age 49, who underwent CTC and CC. CTC was performed with a GE Helical Multislice Computed
Tomography Apparatus. The colonoscopy was performed, in the same day, without previous knowledge of the CTC diagnostics.
The study evaluated the capacity of CTC to detect patients with colorectal polyps and identify each colorectal lesion described by
CC.


*Results:* In two patients (2/21), CC was incomplete. However, in all
patients CTC was complete. In Phase I (“Per...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2323/version/1
Higher accuracy protein Multiple Sequence Alignment by Stochastic Algorithm
Provedor de dados: 17
Autores: M. S. Jeevitesh; Narayan Behera; Justin Jose; Krishna Kant; Alpana Dey.
Multiple Sequence Alignment gives insight into evolutionary, structural and functional relationships among the proteins. Here, a
novel Protein Alignment by Stochastic Algorithm (PASA) is developed. Evolutionary operators of a genetic algorithm, namely,
mutation and selection are utilized in combining the output of two most important sequence alignment programs and then
developing an optimized new algorithm. Efficiency of protein alignments is evaluated in terms of Total Column score which is
equal to the number of correctly aligned columns between a test alignment and the reference alignment divided by the total
number of columns in the reference alignment. The PASA optimizer achieves, on an average, significant better alignment over the
well known...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4258/version/1
FDserver: A web service for protein folding research
Provedor de dados: 17
Autores: Bin-Guang Ma; Ling-Ling Chen; Hong-Yu Zhang.
*Summary:* To facilitate the study of protein folding, we have developed a web service for protein folding rate and folding type
prediction as well as for the calculation of a variety of topological parameters of protein structure, which is freely available to the
community.
*Availability:* http://sdbi.sdut.edu.cn/FDserver
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2349/version/1
Medicine beyond magic bullets: a formal case for multilevel interventions
Provedor de dados: 17
Autores: Rodrick Wallace.
Western medicine's paradigmatic search for 'magic bullet' interventions is facing increasing
difficulty: Between 1950 and 2010 the inflation-adjusted cost per USFDA-approved drug has increased exponentially in time, a
draconian inverse of the famous Moore's Law of computing. A sequence of empirically-oriented statistical models
suggests that carefully designed synergistic multifactorial and multiscale strategies might evade this relationship.
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6780/version/1
Systems Biology Graphical Notation: Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas Le Novere; Anatoly Sorokin; Huaiyu Mi; Falk Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without
electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised
notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields.
With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions
of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few
detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3721/version/1
Description of a method for localizing swarming mosquitoes and other insects in 3D space with
visualizations
Provedor de dados: 17
Autores: Nicholas C. Manoukis.
Male mosquitoes swarm to find mates, but the characteristics of these swarms have not yet been quantitatively analyzed in the
field. This paper describes a simple method used recently to localize individual Anopheles gambiae in a swarm via stereoscopic
image analysis of video footage swarms recorded in Doneguebougou, Mali. The methods described here represent a streamlining
of simple triangulation for insect localization in the hope that other researchers will be able to apply the method for studying
fundamental questions about flying insects or other taxa.

Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2008
URL: http://precedings.nature.com/documents/2599/version/1
On Real-Time Synthetic Primate Vision
Provedor de dados: 17
Autores: Andrew A. Dankers; Nick Barnes; Walter F. Bischof; Alex Zelinsky.
The primate vision system exhibits numerous capabilities. Some important basic visual competencies include: 1) a consistent
representation of visual space across eye movements; 2) egocentric spatial perception; 3) coordinated stereo fixation upon and
pursuit of dynamic objects; and 4) attentional gaze deployment. We present a synthetic vision system that incorporates these
competencies.We hypothesize that similarities between the underlying synthetic system model and that of the primate vision
system elicit accordingly similar gaze behaviors. Psychophysical trials were conducted to record human gaze behavior when
free-viewing a reproducible, dynamic, 3D scene. Identical trials were conducted with the synthetic system. A statistical
comparison of synthetic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2007
URL: http://precedings.nature.com/documents/1241/version/1
Trichoderma reesei derived cellulase activity in three N,N-dimethylethanolammonium
akylcarboxylate ionic liquids
Provedor de dados: 17
Autores: Sierra Rayne; Giuseppe Mazza.
The activity and denaturation extent of cellulase from Trichoderma reesei (E.C. # 3.2.1.4) was investigated in three representative
N,N-dimethylethanolammonium akylcarboxylate ionic liquids. Significant cellulase activity and absence of enzyme unfolding was
found in all concentrations of N,N-dimethylethanolammonium acetate (DMEAA), including the pure liquid. Activities in 20% and
40% (v/v) solutions of DMEAA were equal to citrate buffer controls. Lower enzymatic activities and denaturation were observed
in solutions of the corresponding formate and octanoate ionic liquids, although cellulose hydrolysis still proceeded at a substantial
rate. The results provide the first proof-of-principle that cellulose can be enzymatically hydrolyzed in the presence of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/632/version/1
Systems Biology Graphical Notation: Process Diagram Level 1
Provedor de dados: 17
Autores: Nicolas Le Novere; Stuart Moodie; Anatoly Sorokin; Michael Hucka; Falk Schreiber; Emek Demir; Huaiyu Mi; Yukiko
Matsuoka; Katja Wegner; Hiroaki Kitano.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without
electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised
notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields.
With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions
of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few
detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2320/version/1
A Systematic Approach to Identifying Protein-Ligand Binding Profiles on a Proteome Scale
Provedor de dados: 17
Autores: Li Xie; Nancy Buchmeier; Lei Xie; Philip Bourne.
Identification of protein-ligand interaction networks on a proteome scale is crucial to address a wide range of biological problems
such as correlating molecular functions to physiological processes and designing safe and efficient therapeutics. We have
developed a novel computational strategy to identify ligand binding profiles of proteins across gene families and applied it to
predicting protein functions, elucidating molecular mechanisms of drug adverse effects, and repositioning safe pharmaceuticals to
treat different diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2207/version/1
Facile Synthesis and Versatilities of Polyanthraquinoylamine Nanofibril Bundles with Self Stability
and High Carbon Yield
Provedor de dados: 17
Autores: Xin-Gui Li; Mei-Rong Huang; Hu Li; Mark G. Moloney.
A facile synthesis for nanosized conducting polymers with inherent self-stability and multi-functionalities is a main challenge.
Here we simply synthesize intrinsically self-stabilized nanofibril bundles of poly(1-anthraquinoylamine) (PAQ) by a template-free
method. The critical polymerization parameters were studied to significantly optimize the synthesis, size, properties, and
functionalities of the resulted fine nanofibrils with a diameter of ca. 30 nm and length of ~6 μm. The PAQ obtained
with ammonium persulfate possesses higher polymerization yield, purer composition, higher conductivity, better melting
behaviour, higher thermostability, lower burning enthalpy, and slower degradation than that with other oxidants. Furthermore, the
polymer...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2396/version/1
The Cognitive Atlas: Employing Interaction Design Processes to Facilitate Collaborative Ontology
Creation
Provedor de dados: 17
Autores: Eric Miller; Christian Seppa; Aniket Kittur; Fred W. Sabb; Russell A. Poldrack.
The Cognitive Atlas is a collaborative knowledge-building project that aims to develop an ontology that characterizes the current
conceptual framework among researchers in cognitive science and neuroscience. The project objectives from the beginning
focused on usability, simplicity, and utility for end users. Support for Semantic Web technologies was also a priority in order to
support interoperability with other neuroscience projects and knowledge bases. Current off-the-shelf semantic web or semantic
wiki technologies, however, do not often lend themselves to simple user interaction designs for non-technical researchers and
practitioners; the abstract nature and complexity of these systems acts as point of friction for user interaction, inhibiting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4532/version/1
The Nuclear Imaging Uncertainty Principle. Do Nuclear Cameras Really Work?
Provedor de dados: 17
Autores: Richard Fleming; Gordon Harrington.
The introduction of the Heisenberg Uncertainty principle and Nuclear Cardiology occurred simultaneously in 1925-1927. Thirty
years later the Anger gamma camera would allow for a more sophisticated radioactive isotope counting to determine the presence
or absence of disease. When employed with technetium-99m isotopes, ischemic heart disease can be inferred by differences in
visual appearance of cardiac images. These gestalts of imaging results have been separated from the quantitative information
recorded by the cameras computer. We investigated whether current camera and computer systems are sophisticated enough to
quantify
differences
between
images
to
be
clinically
relevant.
Our
study
demonstrated
that
efforts
to
"sharpen" image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/5013/version/1
Present and future of proteomics data curation at the PRIDE database
Provedor de dados: 17
Autores: Juan Antonio Vizcaino; Henning Hermjakob; Lennart Martens.
Significant progress has been made in improving the accessibility and utility of the large amounts of generated high-throughput
proteomics data by the introduction of publicly available proteomics repositories. One such repository is PRIDE (the
‘PRoteomics
IDEntifications’
database,
"http://www.ebi.ac.uk/pride":http://www.ebi.ac.uk/pride). PRIDE stores mass spectrometry related data,
including
peptide
and
protein
identifications,
mass
spectra
and
valuable
additional
metadata.

At present, data curation in PRIDE is limited to data submission support.
The format in which all submissions need to take place is PRIDE XML. Mass spectrometry derived data is very heterogeneous in
terms of...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3222/version/1
Discovery of transcription factor binding sites through integration of generic motif finders
Provedor de dados: 17
Autores: Edward Wijaya; Siu-Ming Yiu; Thanh Son Ngo; Rajaraman Kanagasabai; Wing-Kin Sung.
Locating transcription factor binding sites is a key step in understanding gene regulation. Due to its importance, many _de novo_
motif-finding methods have been proposed. Individually, these motif finders perform unimpressively overall based on
Tompa's benchmark datasets. Moreover, these motif finders vary in their definitions of what constitute a motif, and in
their methods for finding statistically overrepresented motifs. There is no clear way for biologists to choose the motif finder that is
most suitable for their task. The purpose of this work is to describe a method called MotifVoter to identify transcription factor
binding sites by integrating the results found by motif finders of different models. Validation of our method on...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1251/version/1
Detection of genetic diversity among Indian strains of _Xanthomonas campestris_ pv.
_mangiferaeindicae_ using PCR-RAPD
Provedor de dados: 17
Autores: Ram Kishun; Vijai Kumar Gupta.
The randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of
_Xanthomonas campestris_ pv. _mangiferaeindicae_ (_Xcmi_), the causal pathogen of mango bacterial canker disease (MBCD).
The RAPD analysis was also intended to identify molecular markers, specific to the species to develop PCR-based markers for
detection of _Xcmi_ in mango field and planting materials. Twenty RAPD primers (CP 1-CP 20) were evaluated to establish
molecular characters and genetic variability in the genome of _Xcmi_. Among these, only 4 were found efficient for development
of reproducible banding pattern. It has been observed that the largest and smallest amplified RAPD products were of 2.036 and
0.201 kbp. A total of 136...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2403/version/1
Open Science: Tools, approaches, and implications
Provedor de dados: 17
Autores: Shirley Wu; Cameron Neylon.
The Pacific Symposium on Biocomputing is an annual meeting whose topics are determined by proposals submitted by members
of the community. This document is the proposal for a session on Open Science, submitted for consideration for the PSB meeting
in 2009.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1633/version/1
Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells.
Provedor de dados: 17
Autores: Nathan P. Gabrielson; Daniel W. Pack.
Most in vivo gene therapies will require cell-specific targeting. Although vector targeting through ligand attachment has met with
success in generating gene delivery particles that are capable of specific cellular interactions, little attention has been given to the
possible effects of such ligands on subsequent intracellular processing. In this study, we examine the impact of targeting two
distinct endocytic routes-the caveolar and clathrin pathways-on polyethylenimine-mediated gene delivery in HeLa cells. Targeting
complexes to the caveolar pathway with folic acid and the clathrin pathway with transferrin yields enhanced gene delivery relative
to unmodified polyethylenimine. Colocalization studies with caveolin-1 and clathrin heavy chain indicate that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2262/version/1
Reductive Biotransformation of Ethyl Acetoacetate: A Comparative Studies using Free and
Immobilized Whole Yeast Cells
Provedor de dados: 17
Autores: Amit R. Kumbhar; Vaibhav V. Kulkarni; Madhav B. Pande; Sachin S. Laddha.
Bioreduction of ethyl acetoacetate with free and immobilized yeast whole cell was achieved by using water and sucrose
combination.
After
detachment
from
immobilized
beads
under
basic
condition,
the
corresponding
ethyl(S)-(+)-3-hydroxybutanoate was isolated with 98 to 100% yield. Immobilized beads of yeast whole cell were prepared at
different temperature which affects the morphology and physiology of the beads for the diffusion of the enzyme, which shown the
maximum conversion of the substrate to products as compared to the free yeast whole cell.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2087/version/1
An approach able to escape the most common problem during protein Crystallization i.e. formation of
salt crystal
Provedor de dados: 17
Autores: K. Awaneesh Singh; Patrick Celie; G. R. K. Rao; M. V. Jagannatham.
Formation and differentiation of salt crystal is a most common as well as challenging problem during protein crystallography.
Although there are some methods used for their differentiation i.e. physical manipulation, birefringence, dye absorption and run a
gel but all of them are totally depends on own perception and expertise as well as also require a good crystal (final stage crystal).
To escape this problem the screening was done with PACT, JCSG and other automated screens as everyone do. But during
optimization the additives/salts were ignored at first and optimization screen was made just based on different buffers and
precipitant. After selecting suitable buffer, the type and percentage of precipitant was optimized. We got some ugly crystals and
with...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4725/version/1
Multifunctional Hybrid Silica Particles for Multicolor Imaging and Multiplex Tasking
Provedor de dados: 17
Autores: Sang Man koo; Hae Sung Kim; Chan Yoon Jung; Ha Young Kim; Hoe Jin Hah.
Multifunctional submicron particles have recently emerged as great promises for biological applications such as bio-labeling,
medical diagnostics, and drug delivery. These particles are non-toxic and have size comparable to biomolecules, becoming very
suitable for biomedical applications. The particle structures can be engineered to perform multicolor imaging, as well as multiplex
tasking such as site-selective binding, detection, and separation. In addition, these particles can load a large amount of fluorescent
dye for signal amplification. Several synthetic techniques have been developed for a variety of submicron particles, including
core-shell synthesis, layer-by-layer techniques, multi-block polymer emulsifications, and surface modifications. Silica...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/1621/version/1
Transparent Exopolymer Particles (TEP): an overlooked factor in the process of biofilm formation in
aquatic environments
Provedor de dados: 17
Autores: Tom Berman; Uta Passow.
We hypothesize that transparent exopolymer particles (TEP), present in high concentrations in most sea and freshwaters, are
critical agents for biofilm initiation and development in many natural and anthropogenic aquatic environments. These gel-like
particles appear in many forms; amorphous blobs, clouds, sheets, filaments or clumps ranging in size from ~2 to ~200
µm. TEP are mostly polysaccharide, negatively charged, very sticky and are frequently colonized by bacteria. TEP
may be considered a "planktonic" subgroup of exopolymeric substances (EPS), widely studied in biofilm
research. Recognition of TEP involvement in biofilm formation has important implications for a comprehensive understanding of
the complexities of this...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1182/version/1
Understanding Hydrogen-Bond Patterns in Proteins using a Novel Statistical Model
Provedor de dados: 17
Autores: Ofer Rahat; Uri Alon; Yaakov Levy; Gideon Schreiber.
Proteins are built from basic structural elements and their systematic characterization is of interest. Searching for recurring
patterns in protein contact maps, we found several network motifs, patterns that occur more frequently in experimentally
determined protein contact maps than in randomized contact maps with the same properties. Some of these network motifs
correspond to sub-structures of alpha helices, including topologies not previously recognized in this context. Other motifs
characterize beta-sheets, again some of which appear to be novel. This topological characterization of patterns serves as a tool to
characterize proteins, and to reveal a high detailed differences map for comparing protein structures solved by X-ray
crystallography, NMR and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1490/version/1
An albumin-derived peptide scaffold capable of binding and catalysis
Provedor de dados: 17
Autores: Immacolata Luisi; Silvia Pavan; Giampaolo Fontanive; Alessandro Tossi; Fabio Benedetti; Adriano Savoini; Elisa
Maurizio; Riccardo Sgarra; Daniele Sblattero; Federico Berti.
We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin.
The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues to
form disulfide bridges, which stabilize the polypeptide structure. Seventy-four amino acids are located in six [alpha]-helical
regions, with the remaining amino acids forming six connecting coil/loop regions. Codon usage optimization was used to express
a GST fusion protein in E. coli in yields as high as 4 mg/l. This fusion protein retains its structural integrity and aldolase activity,
the ability to direct the stereochemical outcome of a diketone reduction, and its binding capacity to warfarin and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2012
URL: http://precedings.nature.com/documents/6894/version/1
Affymetrix probes containing runs of contiguous guanines are not gene-specific
Provedor de dados: 17
Autores: Graham J. Upton; William B. Langdon; Andrew P. Harrison.
High Density Oligonucleotide arrays (HDONAs), such as the Affymetrix HG-U133A GeneChip, use sets of probes chosen to
match specified genes, with the expectation that if a particular gene is highly expressed then all the probes in the designated probe
set will provide a consistent message signifying the gene's presence. However, we demonstrate by data mining
thousands of CEL files from NCBI's GEO database that 4G-probes (defined as probes containing sequences of four or
more consecutive guanine (G) bases) do not react in the intended way. Rather, possibly due to the formation of G-quadruplexes,
most 4G-probes are correlated, irrespective of the expression of the thousands of genes for which they were separately intended. It
follows that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1825/version/1
Structural and functional glycosphingolipidomics by glycoblotting with aminooxy-functionalized gold
nanoparticle
Provedor de dados: 17
Autores: Noriko Nagahori; Midori Abe; Shin-Ichiro Nishimura.
Glycosphingolipids (GSLs) synthesized in Golgi apparatus by sequential transfer of sugar residues to a ceramide lipid anchor are
ubiquitously distributing on vertebrate plasma membranes. Standardized method allowing for high throughput structural profiling
and functional characterization of living cell surface GSLs is of growing importance because they function as crucial signal
transduction molecules in various processes of dynamic cellular recognitions. However, methods are not available for
amplification of GSLs, while the genomic scale PCR amplification permits large-scale mammalian proteomic
analysis.　Here we communicate such an approach to a novel "omics", namely
glycosphingolipidomics based on the glycoblotting method....
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2034/version/1
On optical detection of densely labeled synapses in neuropil and mapping connectivity with
combinatorially multiplexed fluorescent synaptic markers
Provedor de dados: 17
Autores: Yuriy Mishchenko.
We propose a new method for mapping neural connectivity optically, by utilizing Cre/Lox system Brainbow to tag synapses of
different neurons with random mixtures of different fluorophores, such as GFP, YFP, etc., and then detecting patterns of
fluorophores at different synapses using light microscopy (LM). Such patterns will immediately report the pre- and post-synaptic
cells at each synaptic connection, without tracing neural projections from individual synapses to corresponding cell bodies. We
simulate fluorescence from a population of densely labeled synapses in a block of hippocampal neuropil, completely reconstructed
from electron microscopy data, and show that high-end LM is able to detect such patterns with over 95% accuracy. We
conclude,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2010
URL: http://precedings.nature.com/documents/4144/version/1
Systems Biology Graphical Notation: Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas Le Novere; Emek Demir; Huaiyu Mi; Falk Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without
electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised
notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields.
With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions
of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few
detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/3721/version/2
Targeted delivery of anti-inflammatory therapy to rheumatoid tissue by fusion proteins containing an
IL-4-linked synovial targeting peptide
Provedor de dados: 17
Autores: Sarah E. Wythe; Danielle DiCara; Taher E. I. Taher; Ciara M. Finucane; Rita Jones; Y. K. Stella Man; Michele
Bombardieri; Ahuva Nissim; Stephen J. Mather; Yuti Chernajovsky; Costantino Pitzalis.
We provide first-time evidence that the synovial endothelium-targeting peptide (SyETP) CKSTHDRLC successfully delivers
conjugated IL-4 to human rheumatoid synovium transplanted into SCID mice. SyETP, previously isolated by in vivo phage
display and shown to preferentially localize to synovial xenografts, was linked by recombinant technology to hIL-4 via an
MMP-cleavable sequence. Both IL-4 and the MMP-cleavable sequence were shown to be functional. IL-4-SyETP augmented
production of IL-1ra by synoviocytes stimulated with IL-1[beta] in a dose-dependent manner. In vivo imaging confirmed
increased retention of SyETP-linked-IL-4 in synovial grafts which was enhanced by increasing number of copies (one to three) in
the constructs. Strikingly, SyETP delivered...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology.
Ano: 2011
URL: http://precedings.nature.com/documents/6399/version/1
Fatty acids composition of some freshwater fish
Provedor de dados: 17
Autores: Chukwuemeka Ugoala; George Ndukwe; Tokunbo Audu.
The aims of this paper were to investigate the component fatty acids of some freshwater fish species by comparing the levels of
essential fatty acids present. The FAME were determined by GC-MS and were identified using retention time locked methods and
retention time databases. The fatty acids profiles include minor amounts of odd-number, branched-chain, and even-number fatty
acids as well as saturated components, the MUFA and PUFA. The major SFA were C14:0 and C16:0. The C18:1 was the
prominent MUFA. The dominant PUFA are of the omega-6 series and are found chiefly in C18:2 fatty acids. The essential fatty
acids compositions showed prominence in C18:3n-3 and C18:2n-6. The branched chain fatty acids identified C15:0, C16:0,
C17:0, C18:2 and C20:0. The...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3239/version/1
Worm-like carbon shell chains produced from wood
Provedor de dados: 17
Autores: Kyoko Suzuki; Hidetoshi Kita; Yukie Saito; Tsutomu Suzuki; Kazuhisa Sato; Toyohiko Konno.
Large-scale utilization of wood which command absolute majority in biomass for functional carbon precursors contributes to
reduce greenhouse effect. Wood char generally has a limit on material usage because of its non-graphitic structure^1^, so we
developed a new functional wood char by iron-, or nickel-catalyzed carbonization, which has a graphite-like structure with
mesopores good for electroconductivity and liquid phase adsorption capacity for macro molecules^2-5^. However the fine
structure of the wood char is still not clear. Here we report more than 70 wt % of iron-catalyzed wood char is filled with chained
carbon shells formed by 3~20 defective stacking layers of carbon hexagonal planes, which look like nanometer-sized worms
swarm. We name them...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6519/version/1
Evaluation of protein surface roughness index using its heat denatured aggregates
Provedor de dados: 17
Autores: Hrishikesh Mishra; Tapobrata Lahiri.
Recent research works on potential of different protein surface describing parameters to predict protein surface properties gained
significance for its possible implication in extracting clues on protein's functional site. In this direction, Surface
Roughness Index, a surface topological parameter, showed its potential to predict SCOP-family of protein. The present work
stands on the foundation of these works where a semi-empirical method for evaluation of Surface Roughness Index directly from
its heat denatured protein aggregates (HDPA) was designed and demonstrated successfully. The steps followed consist, the
extraction of a feature, Intensity Level Multifractal Dimension (ILMFD) from the microscopic images of HDPA, followed by the
mapping of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3693/version/1
Antiviral activity of (E)-cinnamaldehyde revisited with nanoscience tools
Provedor de dados: 17
Autores: Arunava Goswami; Ayesha Rahman.
Contradictory results have been reported regarding the anti-viral activity of (E)-cinnamaldehyde, a major constituent (~69%) of
cinnamon. Here we show that (E)-cinnamaldehyde alone has very low antiviral property contrary to the belief of commoners.
There are early sporadic reports in ancient medicinal practices that fine sand was used for increasing the efficacy of antiviral
drugs. Can we increase the efficacy of (E)-cinnamaldehyde marginally by using one of the major constituents of sand like silica?
Yes, when nanosilica is used as a carrier during (E)-cinnamaldehyde administration, the antiviral efficacy of the resultant cocktail
increases marginally. Therefore, (E)-cinnamaldehyde consumed for centuries in tribal therapy as well as in alternative...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/5043/version/1
The Reproducibility of Lists of Differentially Expressed Genes in Microarray Studies
Provedor de dados: 17
Autores: Leming D. Shi; Wendell D. Jones; Roderick V. Jensen; Stephen C. Harris; Roger G. Perkins; Federico M. Goodsaid; Lei
Guo; Lisa J. Croner; Cecilie Boysen; Hong Fang; Shashi Amur; Wenjun Bao; Catalin C. Barbacioru; Vincent Bertholet; Xiaoxi
Megan Cao; Tzu-Ming Chu; Patrick J. Collins; Xiao-hui Fan; Felix W. Frueh; James C. Fuscoe; Xu Guo; Jing Han; Damir
Herman; Huixiao Hong; Ernest S. Kawasaki; Quan-Zhen Li; Yuling Luo; Yunqing Ma; Nan Mei; Ron L. Peterson; Raj K. Puri;
Feng Qian; Richard Shippy; Zhenqiang Su; Yongming Andrew Sun; Hongmei Sun; Brett Thorn; Yaron Turpaz; Charles Wang;
Sue-Jane Wang; Janet A. Warrington; James C. Willey; Jie Wu; Qian Xie; Liang Zhang; Lu Zhang; Sheng Zhong; Russell D.
Wolfinger; Weida Tong.
Reproducibility is a fundamental requirement in scientific experiments and clinical contexts. Recent publications raise concerns
about the reliability of microarray technology because of the apparent lack of agreement between lists of differentially expressed
genes (DEGs). In this study we demonstrate that (1) such discordance may stem from ranking and selecting DEGs solely by
statistical significance (P) derived from widely used simple t-tests; (2) when fold change (FC) is used as the ranking criterion, the
lists become much more reproducible, especially when fewer genes are selected; and (3) the instability of short DEG lists based
on P cutoffs is an expected mathematical consequence of the high variability of the t-values. We recommend the use of FC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/306/version/2
Building Block and Building Rule: Dual Descriptor Method for Biological Sequence Analysis
Provedor de dados: 17
Autores: Bin-Guang Ma.
The emergence of “Systems Biology” in recent years highlights the systematic viewpoint of
bio-system modeling. Building on such a background, Dual Descriptor Method, a generic methodology for biological sequence
analysis is proposed. From a systematic perspective, Dual Descriptor is defined as a two element set of Composition Weight Map
and Position Weight Function which aim at reflecting the composition and permutation information of a sequence. An alternate
training algorithm is provided to get an optimum description of the building patterns of the sequences. In this paper, dual
descriptor method has been applied to the analysis of two typical problems of molecular biology: gene identification and the
prediction of protein function....
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2223/version/1
Screening Of Antibiotic Producing Microorganism From Rhizoshphere Soil, Their Antibiotic
Production And Characterization
Provedor de dados: 17
Autores: Vishwambhar V. Bhandare; Sandip Mahadev Chavre.
Rhizosphere soil samples were preferred as it contains most of the prospective antibiotic producers. In an attempt to screen out
new potent antibiotic producers from soil, the Rhizobium species was isolated. Antibiotic produced by shake flask culture is found
to be effective against both gram positive and gram negative organisms. This product was found to be a very promising antibiotic
when assayed biologically. UV treatment showed positive effect on antibiotic production. The molecular structure of the antibiotic
was identified by using advanced analytical techniques like UV Visible spectroscopy, FTIR, GCMS, NMR spectroscopy.
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/6101/version/1
Fracture Toughness of Human Solid Cancers
Provedor de dados: 17
Autores: Nagesh Ragavendra; William Klug; J Woody Ju.
Human solid cancers typically are harder and firmer than surrounding normal tissue upon clinical palpation. This characteristic
has been linked to the presence of abundant collagen in the tumor stroma, referred to as the desmoplastic reaction. Recent
evidence indicates that tumor-associated desmoplastic stroma is critical to cancer’s growth and progression.
However, how best to measure, interpret, and/or model the mechanical attributes of tumor microenvironment in vivo remain
unclear. 

During routine sonographically-guided fine needle biopsy of solid tumors of
the human thyroid gland, it was serendipitously observed by the lead author that the nature and strength of the haptic force
feedback cues varied among...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2461/version/1
Secondary water pore formation for proton transport in a ClC exchanger revealed by an atomistic
molecular dynamics simulation
Provedor de dados: 17
Autores: Youn Jo Ko; Won Ho Jo.
Several prokaryotic ClC proteins have been demonstrated to function as exchangers that transport both chloride ions and protons
simultaneously in opposite directions. However, the path of the proton through the ClC exchanger and how the protein brings
about the coupled movement of both ions are still unknown. In the present work, we demonstrate that a previously unknown
secondary water pore is formed inside a ClC exchanger by using an atomistic molecular dynamics (MD) simulation. From the
systematic simulations, it was determined that the glutamate residue exposed to the intracellular solution, E203, plays an
important role as a trigger for the formation of the secondary water pore. Based on our simulation results, we conclude that
protons in the ClC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2168/version/1
Geometric bionics: Lotus effect helps polystyrene nanotube films get good blood compatibility
Provedor de dados: 17
Autores: Chun Mao; Wen-Bo Zhao; Wen-Ping Luo; Cun-Xia Liang; Xiao-Mei Hou; Xiao-Hua Huang; Hong-Ke Liu; Ying-Hong
Xiao; Jian-Chun Bao; Jian Shen.
Various biomaterials have been widely used for manufacturing biomedical applications including artificial organs, medical
devices and disposable clinical apparatus, such as vascular prostheses, blood pumps, artificial kidney, artificial hearts, dialyzers
and plasma separators, which could be used in contact with blood^1^. However, the research tasks of improving
hemocompatibility of biomaterials have been carrying out with the development of biomedical requirements^2^. Since the
interactions that lead to surface-induced thrombosis occurring at the blood-biomaterial interface become a reason of familiar
current complications with grafts therapy, improvement of the blood compatibility of artificial polymer surfaces is, therefore a
major issue in biomaterials...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3002/version/1
Coincidence between transcriptome analyses on different microarray platforms using a parametric
framework
Provedor de dados: 17
Autores: Tomokazu Konishi; Fumikazu Konishi; Shigeru Takasaki; Kohei Inoue; Kouji Nakayama; Akihiko Konagaya.
A parametric framework for the analysis of transcriptome data is demonstrated to yield coincident results when applied to data
acquired using two different microarray platforms. Discrepancies among transcriptome studies are frequently reported, casting
doubt on the reliability of collected data. The inconsistency among observations can be largely attributed to differences among the
analytical frameworks employed for data analysis. The existing frameworks normalizes data against a standard determined from
the data to be analyzed. In the present study, a parametric framework based on a strict model for normalization is applied to data
acquired using an in-house printed chip and GeneChip. The framework is based on a common statistical characteristic of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1746/version/1
The Systems Biology Markup Language (SBML) Level 2 Version 2
Provedor de dados: 17
Autores: Michael Hucka; Sarah M. Keating.
The Systems Biology Markup Language (SBML) is a machine-readable model representation language for software tools in
computational systems biology. By supporting SBML as an input/output format, different tools can all operate on an identical
representation of a model, removing opportunities for translation errors and assuring a common starting point for analyses and
simulations. SBML is by no means a perfect format, but it has achieved widespread acceptance as a de facto standard. It is
supported worldwide by over 100 software systems (both open-source and commercial). The broad acceptance of a common, open
format for exchanging models between software tools is a crucial step towards wider use of quantitative modeling in biology,
because it allows...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/19/version/1
Site-Specific Modification Of Proteins Mediated By Transglutaminase
Provedor de dados: 17
Autores: Abhijeet Satwekar.
Transglutaminase (TGase) catalyzes an acyl transfer reaction between the [gamma]-carboxamide group of glutamine (Gln) and
the [epsilon]-amino group of lysine (Lys) residues to form a stable amide bond. The TGase reaction can be used for
bioconjugation of an amino-derivative of poly-ethylene glycol (PEG) to protein drugs, leading to PEGylated proteins that display
increased bioactivity and stability. The procedure was shown to lead to site-specific bioconjugation of few proteins, thus offering
a valid alternative to the chemical methods of PEGylation in current use (1). Moreover, TGase can be used for site-specifically
labeling proteins with fluorescent groups at the level of Gln or Lys for diagnostic applications. The TGase attack is not entirely
dependent...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6000/version/1
Development of Java based graphical user interface for Diagnosis of Hepatitis UsingI Mixture of
Expert
Provedor de dados: 17
Autores: M Shankaracharya; Suchitra Kumari; Ambarish S. Vidyarthi.
Hepatitis is deadly, and the fifth leading cause of death after heart disease, stroke, chest disease and cancer. Worldwide, 1.5
million deaths per year have been estimated. Detection of hepatitis is a big problem for general practitioners. An expert doctor
commonly makes decisions by evaluating the current test results of a patient or by comparing the patient with others with the
same condition with reference to the previous decisions. Many machine learning and data mining techniques have been designed
for the automatic diagnosis of hepatitis. However, no one tool is available to the general population for the diagnosis of Hepatitis.
Hence, a graphical user interface-enabled tool needs to be developed, through which medical practitioners can feed patient...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7093/version/1
Homology Modeling of Human α~2A~-Adrenoceptor
Provedor de dados: 17
Autores: Jignesh Patel; Gourja G; Vivek Kumar; Yogesh Narkhede; Shikhar Gupta; C. Gopi Mohan.
α~2A~-adrenoceptor (ADA2A) is a membrane bound receptor which has been classified as the member of larger
superfamily G-protein coupled receptors (GPCRs); also known as seven transmembrane (7-TM) domains receptor. Around 50%
drugs currently available in the market exert their effects through GPCRs. Membrane proteins are difficult to crystallize as
compared to soluble proteins. There is a need of 3D-structure of ADA2A to understand
binding modes of various agonists and
antagonists and hence homology model of ADA2A was developed. Homology model of ADA2A constructed based on crystal
structure of β~2~-adrenoceptor. The crystal structure of β~2~-adrenoceptor (PDB ID: 2RH1) was used as
template, which has good sequence identity...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4106/version/1
Emerging Trends of Nanotechnology towards Picotechnology: Energy and Biomolecules
Provedor de dados: 17
Autores: Rakesh Sharma; Avdhesh Sharma; Ching J. Chen.
In nature, metal oxide particles display their existence at the level of picomolecules in solution state and bioactive states in the
body. We present evidence of picomolar behavior of molecules different than nanomolar behavior of particles. These particles can
be encapsulated in polymers and can be functionalized with protein, nucleotides, and drugs to develop as smart intracellular
targeting pico-devices. The preparation technique and physiological conditions decide the size and functionality of these
pico-carrier devices. Their usable success rate, feasibility and potentials are yet to be proven or we do not know. The major
difference between nanodevices and pico-devices is their intermolecular and intramolecular thermodynamics in medium and
their...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4525/version/1
Experimental and theoretical study of DNA oxidation
Provedor de dados: 17
Autores: Yuanzhi Song.
The electrochemical oxidation of DNA and bases at a glassy carbon electrode were investigated using cyclic and square-wave
voltammetry analysis. The predicted oxidative potentials for DNA and bases using DFT method (B3LYP) are in agreement with
the experimental ones.The DNA oxidation ascribes to N-protonated guanine and adenine. The calculated oxidative peaks indicate
that the initial potential of DNA oxidation is around 0.432V for N-protonated guanine and 0.368V (vs.hydrogen standard
electrode) at pH 7.00, and DNA as well as bases in acidity solution are more stable than those in alkalescent solution.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/1797/version/1
Estimation of extracellular lipase enzyme produced by thermophilic bacillus sp. isolated from arid
and semi-arid region of Rajasthan, India
Provedor de dados: 17
Autores: Deeksha
Gaur; Pankaj Kumar
Jain; Yamini Singh Sisodia; Vivek Bajpai.
Thermophilic organisms can be defined as microorganisms which are adapted to live at high temperatures. The enzymes produce
by thermophilic bacteria are capable of catalyzing biochemical reactions at high temperatures. Thermophilic bacteria are able to
produce thermostable lipase enzymes capable of degradation of lipid at temperatures higher than those of mesophilic bacteria.
Therefore, the isolation of thermophilic bacteria from natural sources and their identification are quite useful in terms of
discovering thermophilic lipase enzymes. Due to great temperature fluctuation in hot arid and semi-arid regions of Rajasthan, this
area could serve as a good source for new thermophilic lipase producing bacteria with novel industrially important properties.
The...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7072/version/1
The Importance of Patents for Scientists
Provedor de dados: 17
Autores: Sue Scott.
Patents have become a hot topic and the subject of much media attention. If your main source of information about patents is the
newspapers, you could be forgiven for having an extremely distorted view of what patents are, what they do, and what benefits
and problems are associated with them.
All scientists want to be able to improve the world in some way, whether by
contributing in a fundamental way to the fund of human knowledge or by delivering new and valuable technology. If you are a
scientist making inventions, it is increasingly important that you should have an understanding of the importance of patents in the
modern world, and should have sufficient understanding of the patent system to make sure that your aims for your inventions are...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/1974/version/1
Establishing a multicellular model by three-dimensional cell-assembly technique for metabolic
syndrome
Provedor de dados: 17
Autores: Mingen Xu; Yongnian Yan; Renjie Zhang; Ri Yao; Haixia Liu; Xiaohong Wang.
One of the major obstacles in developing multifunctional drugs for the metabolic syndrome (MS) is lack of in vitro models that
capture more complex features of the disease. Here we give the first report that establishes an energy metabolic system model
using cell-assembly technique which can assemble cells into designated places to form complex three-dimensional structures.
Adipose-derived stromal cells were assembled and induced differentiation into adipocytes and endothelial cells; pancreatic islets
were then deposited at designated locations and constituted adipoinsular axis with adipocytes. Analysis of the factors involved in
energy metabolism showed our system could capture more physiological and pathophysiological features of the in vivo energy...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/1496/version/1
A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and
treatment strategies.
Provedor de dados: 17
Autores: Carl Gregory; Narae Lee; Angela Smolarz; Scott Olson; Odile David; Jacob Reiser; Robert Kutner; Najat Daw; Darwin
Prockop; Edwin Horwitz.
Canonical Wnt signaling is an osteo-inductive signal that promotes bone repair through acceleration of osteogenic differentiation
by progenitors. Dkk-1 is a secreted inhibitor of canonical Wnt signaling and thus inhibits osteogenesis. To examine a potential
osteo-inhibitory role of Dkk-1 in osteosarcoma (OS), we measured serum Dkk-1 in pediatric patients with OS (median age, 13.4
years) and found it to be significantly elevated. We also found that Dkk-1 was maximally expressed by the OS cells at the tumor
periphery and _in vitro_ Dkk-1 and RANKL are co-expressed by rapidly proliferating OS cells. Both Dkk-1 and conditioned
media from OS cells reduces osteogenesis by human mesenchymal cells and by immuno-depletion of Dkk-1, or by adding a
GSK3[beta]...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2007
URL: http://precedings.nature.com/documents/130/version/1
Transforming the Axiomisation of Ontologies: The Ontology Pre-Processor Language
Provedor de dados: 17
Autores: Mikel Egaña Aranguren; Robert Stevens; Erick Antezana.
As ontologies are developed there is a common need to transform them, especially from those that are axiomatically lean to those
that are axiomatically rich. Such transformations often require large numbers of axioms to be generated that affect many different
parts of the ontology. This paper describes the Ontology Pre-Processor Language (OPPL), a domain-specific macro language,
based in the Manchester OWL Syntax, for manipulating ontologies written in OWL. OPPL instructions can add/remove entities,
and add/remove axioms (semantics or annotations) to/from entities in an OWL ontology. OPPL is suitable for applying the same
change to different ontologies or at different development stages, and for keeping track of the changes made (e.g. in pipelines). It
is...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4006/version/1
Adding automated data analysis and biological evaluation to affyanalysisQC
Provedor de dados: 17
Autores: Anwesha Dutta.
Almost every cell in the body contains a full set of chromosomes and identical genes. However, only a fraction of these genes are
turned on, at any given time and it is this subset of genes that are expressed, which confers unique properties to each cell type.
Gene expression is the term used to describe the transcription of the information contained within the DNA into messenger RNA
(mRNA) molecules that are then translated into proteins which perform most of the critical functions of cells.


Gene expression is a highly complex and tightly regulated process that allows a cell
to respond dynamically both to environmental stimuli and to its own changing needs. This mechanism acts as both an on/off
switch to control which...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5969/version/1
The Reproducibility of Lists of Differentially Expressed Genes in Microarray Studies
Provedor de dados: 17
Autores: Leming Shi; Wendell D. Jones; Roderick V. Jensen; Stephen C. Harris; Roger G. Perkins; Federico M. Goodsaid; Lei
Guo; Lisa J. Croner; Cecilie Boysen; Hong Fang; Shashi Amur; Wenjun Bao; Catalin C. Barbacioru; Vincent Bertholet; Xiaoxi
Megan Cao; Tzu-Ming Chu; Patrick J. Collins; Xiao-hui Fan; Felix W. Frueh; James C. Fuscoe; Xu Guo; Jing Han; Damir
Herman; Huixiao Hong; Ernest S. Kawasaki; Quan-Zhen Li; Yuling Luo; Yunqing Ma; Nan Mei; Ron L. Peterson; Raj K. Puri;
Feng Qian; Richard Shippy; Zhenqiang Su; Yongming Andrew Sun; Hongmei Sun; Brett Thorn; Yaron Turpaz; Charles Wang;
Sue-Jane Wang; Janet A. Warrington; James C. Willey; Jie Wu; Qian Xie; Liang Zhang; Lu Zhang; Sheng Zhong; James J. Chen;
Russell D. Wolfinger; Weida Tong.
Reproducibility is a fundamental requirement in scientific experiments and clinical contexts. Recent publications raise concerns
about the reliability of microarray technology because of the apparent lack of agreement between lists of differentially expressed
genes (DEGs). In this study we demonstrate that (1) such discordance may stem from ranking and selecting DEGs solely by
statistical significance (P) derived from widely used simple t-tests; (2) when fold change (FC) is used as the ranking criterion, the
lists become much more reproducible, especially when fewer genes are selected; and (3) the instability of short DEG lists based
on P cutoffs is an expected mathematical consequence of the high variability of the t-values. We recommend the use of FC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/306/version/1
Multifunctional Nanoparticles for Imaging Guided Interventions
Provedor de dados: 17
Autores: Yousef Haik; Basel al-Ramadi; Bashar Issa; Shahnaz Qadri; Saleh Hayek; Hassan Hijaze.
We describe multifunctional magnetic nanoparticles (MNPs) encapsulated in thermosensitive, drug-bearing shells and delivered to
the tumor site by genetically modified and non-pathogenic strains of bacteria with known affinity to tumors for an effective and
minimally invasive protocol for tumor management. The magnetic nanoparticles also serve as a non-invasive imaging contrast
agent, heating agent as well as thermometry monitoring agents. We have shown an efficient tumor management on a mouse model
utilizing the MNPs. Our studies showed that these novel MNPs significantly reduce the progress of tumor and prolong the animal
life and function as an imaging contrast to visually monitor the tumor treatment and evolution.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2190/version/1
Objective dysphonia quantification in vocal fold paralysis: comparing nonlinear with classical
measures
Provedor de dados: 17
Autores: Max A. Little; Declan A. E. Costello; Meredydd L. Harries.
Clinical acoustic voice recording analysis is usually performed using classical perturbation measures including jitter, shimmer and
noise-to-harmonic ratios. However, restrictive mathematical limitations of these measures prevent analysis for severely dysphonic
voices. Previous studies of alternative nonlinear random measures addressed wide varieties of vocal pathologies. Here, we analyze
a
single
vocal
pathology
cohort,
testing
the
performance
of
these
alternative
measures
alongside
classical
measures.

We present voice analysis pre- and post-operatively in unilateral vocal fold
paralysis (UVFP) patients and healthy controls, patients undergoing standard medialisation thyroplasty surgery, using jitter,
shimmer and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3106/version/1
Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral
restoration of vision
Provedor de dados: 17
Autores: Ling Gu; Shivaranjani Shivalingaiah; Mathew Ficinski; Edward Wong; Samarendra Mohanty.
Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for
restoration of vision for treatment of retinitis pigmentosa (RP). Here, we report non-viral in-vivo electroporation of degenerated
retina of adult RP-mice with ChR2-plasmids and subsequent in-vivo imaging of retina to confirm expression. Further, we
demonstrate that in addition to efficient non-viral delivery of ChR2 to a specific retinal layer, threshold level of stimulation light
needs to be delivered onto the retina for achieving successful behavioral outcome. Measurement of intensity of light reaching the
retina of RP-mouse models along with geometrical optics simulation of light propagation in the eye is reported in order to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/6869/version/1
Detection systems cross reacting with peptides - Analysis at the amino acid level
Provedor de dados: 17
Autores: Carsten C. Mahrenholz; Victor Tapia; Rolf Stigler; Rudolf Volkmer.
There are several methods commonly used to measure protein-protein interactions and binding affinities. Quite contrary to most of
these methods, protein- and peptide arrays on cellulose membranes or glass slides are suitable for high-throughput measurement,
as they provide a higher density of probes and a multitude of peptide-protein interactions can be measured in parallel [1]. The
most important application of the SPOT synthesis technique is to simultaneously detect a high number of peptides that have a
strong binding affinity to defined targets. The validity of the results, however, depends on the ability of the detection system to
indicate binding events whilst not interfering with the experiment itself through cross reaction. We tested three common...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4448/version/1
Simulation of Two Dimensional Ultraviolet (2DUV) Spectroscopy of Amyloid Fibrils
Provedor de dados: 17
Autores: Jun Jiang; Darius Abramavicius; Cyril Falvo; Benjamin Bulheller; Jonathan Hirst; Shaul Mukamel.
Revealing the structure and aggregation mechanism of amyloid fibrils is essential for the treatment of over 20 diseases related to
protein misfolding. Coherent two dimensional (2D) infrared spectroscopy is a novel tool that provides a wealth of new insight into
the structure and dynamics of biomolecular systems. Recently developed ultrafast laser sources are extending multidimensional
spectroscopy into the ultraviolet (UV), and this opens up new opportunities for probing fibrils. In a simulation study, we show that
2DUV spectra of the backbone of a 32-residue β-amyloid (Aβ(9-40)) fibril associated with
Alzheimer's disease, and two intermediate prefibrillar structures carry characteristic signatures of fibril size and
geometry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4369/version/1
Expression profile of genes involved in hydrogen sulphide liberation by _Saccharomyces cerevisiae_
grown under different nitrogen concentrations
Provedor de dados: 17
Autores: Ana Mendes-Ferreira; Catarina Barbosa; Marcel.lí del Olmo; Arlete Mendes-Faia; Cecília
Leão.
The present work aims to elucidate molecular mechanisms underlying hydrogen sulphide production in _S. cerevisiae_ associated
to nitrogen deficiency. To assess, at a genome-wide level, how the yeast strain adapted to the progressive nitrogen depletion and
to nitrogen re-feeding, gene expression profiles were evaluated during fermentation at different nitrogen concentrations, using the
DNA array technology. The results showed that most MET genes displayed higher expression values at the beginning of both
control and N-limiting fermentation, just before the time at which the release of sulphide was observed. MET genes were
downregulated when yeast stopped growing which could associate MET gene expression levels with cell growth. The over
expression of MET...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2736/version/1
MIREOT: the Minimum Information to Reference an External Ontology Term
Provedor de dados: 17
Autores: Mélanie Courtot; Frank Gibson; Allyson L. Lister; James Malone; Daniel Schober; Ryan R. Brinkman; Alan
Ruttenberg.
While the Web Ontology Language (OWL) provides a mechanism to import ontologies, this mechanism is not always suitable.
First, given the current state of editing tools and the issues they have working with large ontologies, direct OWL imports have
sometimes proven impractical for day-to-day development. Second, ontologies chosen for integration may be under active
development and not aligned with the chosen design principles. Importing heterogeneous ontologies in their entirety may lead to
inconsistencies or unintended inferences. In this paper we propose a set of guidelines for importing required terms from an
external resource into a target ontology. We describe the guidelines, their implementation, present some examples of application,
and outline...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3576/version/1
The Simulation Experiment Description Markup Language - Update
Provedor de dados: 17
Autores: Frank T. Bergmann.
This presentation gives a brief overview of the Simulation Experiment Description Markup Language L1V1 as well as an
introduction to LibSedML (http://libsedml.sf.net) a library for reading and writing SED-ML documents.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5589/version/1
The Systems Biology Markup Language (SBML): Where It's Been and Where
It's Going
Provedor de dados: 17
Autores: Michael Hucka.
A cornerstone of systems biology is the use of computational modeling, by which hypotheses can be cast into a quantitative form
that can be tested systematically. The use of computational modeling by biologists promises to pave the way for more rigorous
analyses
of
biological
functions,
and
ultimately
will
lead
to
new
and
better
treatments
for
disease.

A crucial enabler for more widespread use of computational modeling in
biology is reaching agreement on how to represent, store, and communicate models between software tools. The Systems Biology
Markup Language (SBML) project is an effort to create a machine-readable format for representing computational models in
biology. By supporting SBML as an input and output...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/21/version/1
Fluorescence Correlation Spectroscopy as a Quantitative Tool Applied to Drug Delivery Model
Systems
Provedor de dados: 17
Autores: Christoph Jung; Sigrun Polier; Markus Schoeffel; M Drechsler; Valerie Jerome; Ruth Freitag.
The delivery of drugs to cells is a very active area of research. Drugs are commonly believed to ameliorate illnesses. The targeted,
controlled and/or enhanced uptake of the drug into cells is facilitated by loading the drug into carrier vehicles (nanoparticles, e.g..
micelles).The distribution of a drug over the nanoparticles used to deliver the drug to the targeted cells is of vital importance. It
makes a difference, whether all nanoparticles carry the same amount of drug or whether a little amount of the nanoparticles carries
a high proportion of the drug material and most nanoparticles carry no material. This depends on the method used to load the
drugs into the carriers. Furthermore, the absolute amount of drug present in the nanoparticles and the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4140/version/1
Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio
neocaledonicus sp nov., from New Caledonia
Provedor de dados: 5
Autores: Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir,
H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle.
Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the
techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and
Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection
of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify
NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration
process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic
Resonance and HPLC-size...
Tipo: Text
Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf
A nationwide biotechnology outreach and awareness program for Malaysian high schools
Provedor de dados: 69
Autores: Firdaus-Raih,Mohd; Senafi,Sahidan; Munir Murad,Abdul; Marzuki Sidik,Nik; Kiew Lian,Wan; Daud,Fauzi; Zainal
Ariffin,Shahrul Hisham; Zamrod,Zulkeflie; Chon Seng,Tan; Sofiman Othman,Ahmad; Azni Harmin,Sharr; Radzuan Saad,Mohd
Yusof; Mohamed,Rahmah.
Biotechnology education in developing nations remains one of the rate limiting factors in achieving optimal human resource
capacity to drive and tap the bio-resources of these nations. Many developing countries are situated within rich bio-diversity
enclaves. Biotechnology offers the promise of tapping these bio resources towards due process of developing these nations. While
there may be a steady stream of biology and biotechnology based graduates, from Malaysian as well as foreign universities
contributing to the human resource base for these countries, the numbers and knowledge diversity produced, still lack the capacity
to optimally power research and development as well as supply the industrial biotechnology sectors of these countries. Realizing
the...
Tipo: Journal article
Palavras-chave: Biotechnology awareness; Biotechnology education; Capacity building.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100004
Strategic approaches to informing the public about biotechnology in Latin America
Provedor de dados: 69
Autores: Traynor,Patricia L; Adonis,Marta; Gil,Lionel.
Abstract
<a
href="http://fbpe/img/ejb/v10n2/a01/reprint.html">Reprint
(PDF)</a>
The
benefits
of
today's
biotechnology products are not evident to consumers. The public will accept biotechnology only when individuals decide for
themselves that biotec products will contribute to their personal well-being. To make such a decision, people will need greater
awareness and understanding of how biotechnology will affect the environment, human health, local and national economies, and
the well-being of society. A low level of awareness and understanding about biotechnology is characteristic of Latin America and
the Caribbean countries, as elsewhere, efforts to remedy poor public perception often seem inadequate and do not reflect a
well-designed...
Tipo: Journal article
Palavras-chave: Biotechnology; GMOs; Public perception.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200001
Plant biotechnology
Provedor de dados: 28
Autores: S, Nagarajan.
Realizing the importance of agriculture to the Indian economy and in accelerat- ing the process of development, a special section
of the Current Science1 was re- cently brought out on the application of plant biotechnology in crop improve- ment. The
reviewers have made several useful observations explaining the insti- tutional framework and codal proce- dures, review of the
progress, indexing the plant breeder’s perceptions on the is- sues to be tackled, advances in the engi- neering of host plant
resistance, the awaited improvements in the plant vari- ety act, and positioning India in the context of the ongoing global
R&D envi- ronment. In fact, all those who were assigned the job have done an exceed- ingly good job. My attempt here is to
do the...
Tipo: Article
Palavras-chave: Agricultural Sciences and Technology; Biotechnology.
Ano: 2003
URL: http://eprints.iari.res.in/68/1/121a.pdf
Molecular marker based linkage map of chickpea (Cicer arietinum)developed from desi × kabuli
cross
Provedor de dados: 28
Autores: BHARADWAJ, C; CHAUHAN , S K; SHUBHA , YADAV; TARA SATYAVATHI, C; RAKESH , SINGH; KUMAR,
J; RACHANA , SRIVASTAVA; GAYATRI , RAJGURU.
BGD 112’ and ‘FLIP 90–166’ using Sequence-tagged Microsatellite (STMS) Markers. Both the parents representing the
cultivated chickpea, the map loci marked indicates usable polymorphism for genetic as well as loci studies. A total of 250 STMS
markers were used to study the parental polymorphism and 49 of which had showed polymorphism were used for genotyping F2
lines. Linkage analysis revealed 8 linkage groups mapped by 33 loci by these markers covering a distance of 471.1 cM of map
distance with an average marker density of 14.2 cM at a LOD of 3.0. The molecular map using desi × kabuli cross throws insights
into variability and diversity that can be utilized directly by the breeders unlike that generated using wide crosses as the loci that
this map has...
Tipo: Article
Palavras-chave: Agricultural Sciences and Technology; Biotechnology.
Ano: 2011
URL: http://eprints.iari.res.in/320/1/Mol_map_chb.pdf
“Molecular Marker-Assisted Crop Breeding: Principles, Methods and Applications”
Provedor de dados: 28
The science of genetics that surfaced in the 19th century is the core of all biological researches now. It is still growing and the new
developments are accumulating at an unprecedented rate. Its core principles have been effectively utilized for genetic
improvement of the crop species resulting in increased harvest with good quality. However, to meet the increasing demand for
food crops, it becomes imperative for interdisciplinary approaches where genetics and plant breeding shall play the key role. With
the recent advances in biotechnology, information technology, software-assisted quantitative genetics, crop modeling, etc. have
opened new vistas for genetic dissection of complex traits of economic importance in crop plants. Similarly, the information...
Tipo: Book
Palavras-chave: Agricultural Sciences and Technology; Biotechnology.
Ano: 2010
URL: http://eprints.iari.res.in/327/1/Molecular_Breeding_Manual.doc
NÚCLEO de Biotecnologia Aplicada a Cereais de Inverno: NBAC - Embrapa Trigo.
Provedor de dados: 14
Genética molecular; Biologia celular; Cultivo de trigo in vitro para obtenção de plantas duplo-haplóides; Tranformação genética;
Citogenética molecular; Bioinformática.
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Biotechnology; Planta transgênica.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/842836
Glossario de biotecnologia.
Provedor de dados: 14
Autores: TORRES, A. C.; FERREIRA, A. T.; BUSO, J. A.; ROMANO, E.
1999
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Biotecnologia; Glossario; Biotechnology; Glossary.
Ano: 1999
Biotechnology international agricultural research: proceedings of the Inter-Center Seminar on
International Agricultural Research Center (IARCs) and Biotechnology (1984 : IRRI)
Provedor de dados: 34
Autores: International Rice Research Institute.; Inter-Center Seminar on International Agricultural Research Centers (IARCs) and
Biotechnology (1984 : IRRI).
455 p. : ill.
Tipo: Book
Palavras-chave: Biotechnology; Research; World.
Ano: 1985
URL: http://hdl.handle.net/10269/282
Harnessing Aquatic Yeasts For Developing A New Generation of Sunscreens
Provedor de dados: 17
Autores: Hycienth Braganca; Nandkumar Kamat.
Around 100 yeast genera and 800 species have been isolated and characterized. Nagahama (2006) has reviewed yeast biodiversity
in freshwater, marine and deep-Sea Environments. Our work aims at systematic biodiversity assessment of freshwater aquatic
yeasts from Goa with major focus on carotenogenic basidiomycetes yeasts which on exposure to potentially damaging levels of
solar ultraviolet radiation (UVR) accumulate photoprotective compounds (PPC) such as mycosporine-glutaminol-glucoside
(myc-glu-glu, peak absorption at 309-310nm) that server as sunscreens and /or antioxidant. These yeasts are a potential source of
a particularly diverse family of such substances, collectively referred to as mycosporine- like amino-acids (MAAs). This paper
presents the...
Tipo: Poster
Palavras-chave: Biotechnology; Ecology; Microbiology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4249/version/1
Slug-based epithelial-mesenchymal transition gene signature is associated with prolonged time to
recurrence in glioblastoma
Provedor de dados: 17
Autores: Wei-Yi Cheng; Jessica J. Kandel; Darrell J. Yamashiro; Peter Canoll; Dimitris Anastassiou.
Background
We previously identified a precise stage-associated gene expression signature of coordinately
expressed genes, including the transcription factor Slug (SNAI2) and other epithelial mesenchymal transition (EMT) markers,
present in samples from publicly available gene expression datasets in multiple cancer types. The expression levels of the
co-expressed genes vary in a continuous and coordinate manner across the samples, ranging from absence of expression to strong
co-expression of all genes. These data suggest that tumor cells may pass through an EMT like process of mesenchymal transition
to varying degrees. 

Findings
Here we show that this signature
in glioblastoma multiforme...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6544/version/1
The History, Development and Impact of Computed Imaging in Neurological Diagnosis and
Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed
diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the
advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities,
forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama
of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/2
Enhancing RNAi efficiency by inserting Nuclear factor-κB binding sequence into SiRNA
expression cassette
Provedor de dados: 17
Autores: Jianzhong Yi; Chenqian Liu.
RNAi based applications have been widely used to manipulate cellular phenotypes, analyze gene function and as a promising
molecular therapy, however, there are rare reports in how to target the SiRNA expression constructs from the cytoplasm to
nucleus. In this study, we inserted NF-κB binding sequence into the SiRNA expression cassette to enhance RNAi
efficiency, tested this propose by use of tRNAval and human H1 promoters, and eGFP and luciferase as reporter protein. The
results showed that inserting NF-κB binding sequence at the 3’-terminal of the SiRNA expression
construct could enhance RNAi efficiency.
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4283/version/1
Detection of Metabolites by Proton Ex Vivo NMR, in Vivo MR Spectroscopy Peaks and Tissue
Content Analysis: Biochemical-Magnetic Resonance Correlation: Preliminary Results
Provedor de dados: 17
Autores: Rakesh Sharma; Madan Rehani; Arun Agrawala.
*Aim*: Metabolite concentrations by in vivo magnetic resonance spectroscopy and ex vivo NMR spectroscopy were compared
with
excised
normal
human
tissue
relaxation
times
and
tissue
homogenate
contents.

*Hypothesis*: Biochemical analysis combined with NMR and MR
spectroscopy defines better tissue analysis.

*Materials and Methods*: Metabolites
were measured using peak area, amplitude and molecular weights of metabolites in the reference solutions. In normal brain and
heart autopsy, muscle and liver biopsy tissue ex vivo NMR peaks and spin-lattice (T1) and spin-spin (T2) relaxation times, were
compared with diseased tissue NMR data in meningioma brain, myocardial infarct heart,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4121/version/1
Design of selective peptide antibiotics by using the sequence moment concept
Provedor de dados: 17
Autores: Davor Juretic; Nada Ilic; Damir Vukicevic; Nikolinka Antcheva; Alessandro Tossi.
New antibiotics against multidrug-resistant bacteria are urgently needed, but rapid acquisition of resistance limits their usefulness.
Endogenous antimicrobial peptides (AMPs) with moderate selectivity, but multimodal mechanism of action, have remained
effective against bacteria for millions of years. Their therapeutic application, however, requires optimizing the balance between
antibacterial activity and selectivity, so that rational design methods for increasing selectivity are highly desirable. We have
created training (n=36) and testing (n=37) sets from frog-derived AMPs with determined therapeutic index (TI). The
'sequence moments' concept then enabled us to find a one-parameter linear model resulting in a good
correlation between...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3068/version/1
Structure-based design of novel pyrazoles as aurora kinase A inhibitors.
Provedor de dados: 17
Autores: Mohane Coumar; Paritosh Shukla; Ajay Kumar Dixit; Jiun-Shyang Leou; John T. A. Hsu; Su-Ying Wu; Hsing-Pang
Hsieh.
Inhibition of Aurora kinase, a member of serine/threonine kinase involved in the regulation of cell division is emerging as a new
molecular targeted cancer treatment option. Three isoforms of Aurora kinase, A, B and C are known. Both Aurora A and B are
over expressed in many human cancers and are linked to chromosome instability, oncogenic transformation, tumour progression
and development of chemoresistance. Inhibitors of Aurora kinase, regardless of their kinase specificity spectrum have shown to
promote cancer cell death by induction of apoptosis and mitotic catastrophe. Based on the current success of Aurora kinase
inhibitors in the development of kinase-based cancer therapy, we have initiated a structure-based virtual screening for the
identification...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/2769/version/1
Failure of Abeta(1-40) amyloid fibrils under tensile loading
Provedor de dados: 17
Autores: Raffaella Paparcone; Markus J. Buehler.
Amyloid fibrils and plaques are detected in the brain tissue of patients affected by Alzheimer’s disease, but have
also been found as part of normal physiological processes such as bacterial adhesion. Due to their highly organized structures,
amyloid proteins have also been used for the development of novel nanomaterials, for a variety of applications including
biomaterials for tissue engineering, nanolectronics, or optical devices. Past research on amyloid fibrils resulted in advances in
identifying their mechanical properties, revealing a remarkable stiffness. However, the failure mechanism under tensile loading
has not been elucidated yet, despite its importance for the understanding of key mechanical properties of amyloid fibrils and
plaques...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5174/version/1
A call for disruptive innovation in science publishing with a new open data-sharing platform for the
life sciences
Provedor de dados: 17
Autores: Donald C. Cooper.
“A disruptive innovation is an innovation that helps create a new market and value network, and eventually goes on
to disrupt an existing market and value network (over a few years or decades), displacing an earlier technology. The term is used
in business and technology literature to describe innovations that improve a product or service in ways that the market does not
expect.”
-Wikipedia
On
April
3rd,
2012
Nature
Precedings,
Nature
Publishing
Group’s experiment in free pre-print publishing was shut down and no longer accepts submissions. According to the
Nature Precedings website it was created in 2007 as “a place for researchers to share documents, including
presentations, posters, white...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7151/version/1
An innovative method for the biological control of Alternanthera philoxeroides
Provedor de dados: 17
Autores: Yusong Cao; Huiming Wang.
Biological invasion has become a serious global environmental problems and been considered as an important component of
global change^1-2^. Invasions by alien species can have an impact at several levels of ecological complexity from genes to
ecosystems^3-4^. Invasive alien species had seriously threated the biodiversity and natural ecosystems in China^5^, and caused
economic losses by more than US$7 billion every year^6^. Alternanthera philoxeroides, commonly known as Alligator weed, is
an invasive amphibious plant^7^. The effect of physical methods such as mechanical or artificial salvage, removal of A.
philoxeroides were very limited. Moreover, these measure should be carried out very carefully or would easy lead to its
rebound^8^. The mainly biological...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5267/version/1
Mobile Image Ratiometry: A New Method for Instantaneous Analysis of Rapid Test Strips
Provedor de dados: 17
Autores: Donald C. Cooper; Bryan Callahan; Phil Callahan; Lee Burnett.
Here we describe Mobile Image Ratiometry (MIR), a new method for the automated quantification of standardized rapid
immunoassay strips using consumer-based mobile smartphone and tablet cameras. To demonstrate MIR we developed a
standardized method using rapid immunotest strips directed against cocaine (COC) and its major metabolite, benzoylecgonine
(BE). We performed image analysis of three brands of commercially available dye-conjugated anti-COC/BE antibody test strips in
response to three different series of cocaine concentrations ranging from 0.1 to 300 ng/ml and BE concentrations ranging from
0.003 to 0.1 ng/ml. These data were then used to create standard curves to allow quantification of COC/BE in biological samples.
MIR quantification of COC and BE...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6827/version/1
VO: Vaccine Ontology
Provedor de dados: 17
Autores: Yongqun He; Lindsay Cowell; Alexander D. Diehl; Harry Mobley; Bjoern Peters; Alan Ruttenberg; Richard H.
Scheuermann; Ryan R. Brinkman; Melanie Courtot; Chris Mungall; Zuoshuang Xiang; Fang Chen; Thomas Todd; Lesley
Colby; Howard Rush; Trish Whetzel; Mark A. Musen; Brian D. Athey; Gilbert S. Omenn; Barry Smith.
Vaccine research, as well as the development, testing, clinical trials, and commercial uses of vaccines involve complex processes
with various biological data that include gene and protein expression, analysis of molecular and cellular interactions, study of
tissue and whole body responses, and extensive epidemiological modeling. Although many data resources are available to meet
different aspects of vaccine needs, it remains a challenge how we are to standardize vaccine annotation, integrate data about
varied vaccine types and resources, and support advanced vaccine data analysis and inference. To address these problems, the
community-based Vaccine Ontology (VO,...
Tipo: Poster
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3552/version/1
Life fingerprints of nuclear reactions in the body of animals
Provedor de dados: 17
Autores: Jun Zeng; Ji-hui Han; Tao Sun; Ji-hui Su; Qi-yin Sun; Quan-shi Zhang; Wen-hui Xie; Ci-yi Liu; Xiao-jia Cai.
Nuclear reactions are a very important natural phenomenon in the universe. On the earth, cosmic rays constantly cause nuclear
reactions. High energy beams created by medical devices also induce nuclear reactions in the human body. The biological role of
these nuclear reactions is unknown. Here we show that the in vivo biological systems are exquisite and sophisticated by nature in
influence on nuclear reactions and in resistance to radical damage in the body of live animals. In this study, photonuclear reactions
in the body of live or dead animals were induced with 50-MeV irradiation. Tissue nuclear reactions were detected by positron
emission tomography (PET) imaging of the induced beta+ activity. We found the unique tissue...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4897/version/1
Transgene effects on rhizodeposition: Evidence from molecular-chemical screening by Pyrolysis-Field
Ionisation Mass Spectrometry (Py-FIMS)
Provedor de dados: 17
Autores: Andre Schlichting; Christel Baum; Dana Zimmer; Peter Leinweber.
Rhizodeposition plays an important role in the C and N cycle of ecosystems, since it essentially reflects the interaction between
plant, soil and soil microorganisms. The molecular-chemical characterisation of rhizodeposition is often limited to selected
compound classes (e.g., carbohydrates and amino acids). A more comprehensive analytical approach is based on the
molecular-chemical “fingerprint” revealed by Pyrolysis-Field Ionisation Mass Spectrometry
(Py-FIMS).

The presented results give evidence of specific effects in conjunction
with soil type, crop, variety, transgenes and mycorrhizal colonisation on the molecular-chemical composition of rhizodeposition.
The experiments included various crops...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4440/version/1
_Streptomyces_ sp. as predators of bacteria
Provedor de dados: 17
Autores: Charushila A. Kumbhar; Milind G. Watve.
Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood.

Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in
soil. 
Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis
accompanying
growth.
Circumstantial
evidence
for
the
involvement
of
antimicrobials
along
with
enzymes.
Implications: This finding may open up a new source of novel secondary metabolites from the
genus.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1263/version/1
BioSharing: Standards, Policies and Communication in Bioscience
Provedor de dados: 17
Autores: Dawn Field; Susanna-Assunta Sansone; Philippe Rocca-Serra.
BioSharing works at the global level to build stable linkages between journals, funders, implementing data sharing policies, and
well-constituted standardization efforts in the biosciences domain, to expedite the communication and the production of an
integrated standards-based framework for the capture and sharing of high-throughput genomics and functional genomic bioscience
data.
Tipo: Presentation
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6436/version/1
Antibody-antigen interactions: What is the required time to equilibrium?
Provedor de dados: 17
Autores: Karl Andersson; Hanna Björkelund; Magnus Malmqvist.
The use of antibodies is widespread in many areas including in-vivo and in-vitro diagnostics, quantitative analysis in research
laboratories and as therapeutic substances. Since the methods for generation of antibodies has improved and regularly results in
high-affinity interactions, the standard assays used for quantification of the interaction properties should be revisited because they
do not necessarily produce accurate results. Here we show that in several cases, the affinity determination of strongly binding
antibodies will be inherently difficult when using standard procedures, due to impractically long incubation times. Real-time
kinetic analysis is often the only realistic alternative for affinity determination.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/5218/version/1
Intra-mammary single infusion of casein hydrolyzate in dairy cows as alternative dry period
treatment: Curing bacterial infection and increasing milk yield in the subsequent lactations
Provedor de dados: 17
Autores: Nissim Silanikove; Shamay Yacoby; Gabriel Leitner.
Conclusions: This study sustain previous results that treatment with CNH is a powerful mean to eradicated bacterial infection, and
strongly highlight the potential of this method to serve as a viable non-antibiotic dry cow therapy treatment. The increase in milk
yield is a valuable bonus and its effectiveness is comparable with that achieved with growth hormone treatment, albeit in much
simple mode of treatment and without the problematic of hormonal treatment.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2130/version/1
A prelude report on molecular docking of HER2 protein towards comprehending anti-cancer
properties of saponins from Solanum tuberosum
Provedor de dados: 17
Autores: Puneet K. Singh; Pratyoosh Shukla.
Saponins are extensively known for many biological activities e.g. antimicrobial, anti-palatability, anti-cancer and hemolytic. As
cancer cells have a more cholesterol-like compound in their membrane structure the saponins bind cholesterol due to their natural
affinity to bind cancer cell membrane. This prevents them from entering the body through the intestinal tract, where they have the
ability to attach themselves to vital organs and grow. This study reports the effective use of lower dose saponins like immunotoxin
so that they can inhibit the proliferation of cancerous pancreatic cells. The investigation of pancreatic cancer metabolic pathway it
was found that proteins 3H3B produced by genes HER-2 are involved in the enhancement of this type of cancer....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7147/version/1
Induced fit, conformational selection and independent dynamic segments: an extended view of
binding events
Provedor de dados: 17
Autores: Peter Csermely; Robin Palotai; Ruth Nussinov.
Single molecule and NMR measurements of protein dynamics increasingly uncover the complexity of binding scenarios. Here we
describe an extended conformational selection model which embraces a repertoire of selection and adjustment processes. Induced
fit can be viewed as a subset of this repertoire, whose contribution is affected by the bond-types stabilizing the interaction and the
differences between the interacting partners. We argue that protein segments whose dynamics are distinct from the rest of the
protein (‘discrete breathers’) can govern conformational transitions and allosteric propagation that
accompany binding processes, and as such may be more sensitive to mutational events. Additionally, we highlight the dynamic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4422/version/1
_Streptomyces_ sp. as predators of bacteria
Provedor de dados: 17
Autores: Charushila A. Kumbhar; Milind G. Watve.
Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood.

Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in
soil. 
Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis
accompanying
growth.
Circumstantial
evidence
for
the
involvement
of
antimicrobials
along
with
enzymes.
Implications: This finding may open up a new source of novel secondary metabolites from the
genus.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1263/version/2
Development of a sampling regime for research with environmental relevance based on variability of
transgene expression in plants
Provedor de dados: 17
Autores: Carla Struzyna-Schulze; Heike Mikschofsky; Wenke Mönkemeyer; Jörg Schmidtke; Kerstin
Schmidt; Inge Broer.
Transgenic plants often produce proteins that are unknown in conventional crops. Therefore, it requires a fundamental analysis
before approval of a new genetically modified plant is given. Since the EFSA-guidelines for risk assessment on transgenic plants
provide only recommendations but no detailed instructions, we wanted to develop a valid test scheme that defines sample size and
sampling organ to enable a statically valid and efficient analysis of transgene expression and its variability. The scheme will be
based on data obtained for the variability in expression i. of different recombinant proteins in one variety; ii. of different
integration sites of one transgene in one variety; iii. of the same recombinant protein between different varieties and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4443/version/1
Trypsin digest of cancer cells surface stimulates anti-tumor immune response better than cancer cells
themselves
Provedor de dados: 17
Autores: Petr Lokhov; Balashova Elena.
Antigens expressed on the surface of cancer cells are accessible targets for both humoral and cell-mediated immune responses,
and are therefore potential candidates for vaccine development. Treating surface of live human breast adenocarcinoma cells
(MCF-7) with trypsin yields a digest that contains 0.7% of total cell protein. Despite this difference, the trypsin digest stimulates
in cytotoxicity assays anti-tumor response which kills 10-40% more cancer cells than those stimulated with cells themselves.
From these results, we concluded that trypsin digest obtained from live cancer cells contains the essential antigens to induce an
immune-mediated anti-tumor effect, and therefore, is candidate for anti-tumor vaccine development.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2010
URL: http://precedings.nature.com/documents/4895/version/1
A new outlook towards kidney injuries
Provedor de dados: 17
Autores: Mahesh Satwekar; Abhijeet Satwekar.
Acute and chronic progression of injury to the kidney leads to the failure of the renal system and has become an increasingly
important cause of morbidity and mortality. Present diagnosis detects the condition only after irreversible loss of 70 percent of
kidney function. Current research is focused only on the clinical manifestations after the kidney injuries and not towards the exact
cause of the condition. Here we propose a new outlook- that there is an involvement of a pathogen in the pathogenesis of kidney
injuries. Basis for our proposal is given by the similarity of the pathogenesis events occurring between a classical example of
hepatitis and kidney injuries. Furthermore, literature regarding the role of early kidney injury biomarkers in innate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7021/version/1
Tendril, adhesive disc and super adhesive effect of climbing plant
Provedor de dados: 17
Autores: Wenli Deng.
Understanding the super adhesion mechanism of biological system is of great scientific interest and a prerequisite for bioinspired
design of adhesive systems. To investigate the versatile climbing plant requires the development of new methods. Here I present
strategies to fully study the super adhesive effect of climbing plant _Parthenocissus tricuspidata_ by the first time measuring the
mass and the attached area of single adhesive disc, by further determining the microscopic structure and the adhesive strength of
single adhesive disc and by finally elucidating the adhesion mechanism in cellular and molecular level using the classical theories
and the proposed new hypothesis and model. I have measured that a single mature adhesive disc has an average mass...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1513/version/1
Evolving standards and infrastructure for systems biology: SBML, SBGN, and BioModels.net
Provedor de dados: 17
Autores: Michael Hucka.
Systems biology has arisen through the convergence of theoretical, computational, and mathematical modeling of systems and the
need to understand the wealth of information being rapidly generated in biology. Systems biology by its nature requires
collaborations between scientists with expertise in biology, chemistry, computer sciences, engineering, mathematics, and physics.
Successful integration of these disciplines depends on bringing to bear both social and technological tools: namely, consortia that
help forge collaborations and common understanding, software tools that permit analysis of vast and complex data, and
agreed-upon standards that enable researchers to communicate and reuse each other's results in practical and
unambiguous ways....
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2007
URL: http://precedings.nature.com/documents/20/version/1
The History, Development and Impact of Computed Imaging in Neurological Diagnosis and
Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed
diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the
advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities,
forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama
of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/4
Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and
Photoactivation of cyan fluorescent proteins at YFP-Excitation
Provedor de dados: 17
Autores: Johannes Schmid; Naila Malkani.
Background
The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or
yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific
proteins or cells or to determine protein interactions. In the latter case, the quantum physical phenomenon of fluorescence
resonance
energy transfer
(FRET)
is exploited
by specific
microscopy techniques to
visualize
proximity of
proteins.

Methodology/Principal Findings
When we applied a
commonly used FRET microscopy technique - the increase in donor (CFP)-fluorescence after bleaching of acceptor fluorophores
(YFP), we obtained good signals in live cells, but very...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/4517/version/2
HBsAg-vectored DNA vaccines elicit concomitant protective responses to multiple CTL epitopes
relevant in human disease.
Provedor de dados: 17
Autores: Dekun Chen; Allan Gould; Michael Mather; Oscar Haigh; Melanie Barnes; Jacqueline Kattenbelt; Scott Thomson;
Robert R. Tindle.
Vaccines capable of controlling neoplastic and infectious diseases which depend on the cellular immune response for their
resolution, have proven difficult to develop. We, and others, have previously demonstrated that the potent immunogenicity of
hepatitis B surface antigen (HBsAg), the already- licensed human vaccine for hepatitis B infection, may be exploited to deliver
foreign antigens for cytotoxic T-lymphocyte (CTL) induction. In this study we demonstrate that recombinant (r) HBsAg DNA
delivering a CTL polyepitope appended at the C' terminus elicits concomitant responses to multiple epitopes restricted
through a diversity of MHC class I haplotypes, which are relevant in a number of human diseases. We show that the rHBsAg
DNA vaccine elicits...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2008
URL: http://precedings.nature.com/documents/1910/version/1
Controlling sap-sucking insect pests with recombinant endophytes expressing plant lectin
Provedor de dados: 17
Autores: Xiuyun Zhao; Gaofu Qi; Xianfang Zhang; Nan Lan; Xinrong Ma.
We developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins. Fungal endophyte of
Chaetomium globosum YY-11 with anti-fungi activities was isolated from rape seedlings, and bacterial endophytes of SJ-10
(Enterobacter sp.) and WB (Bacillus subtilis) were isolated from rice seedlings. Pinellia ternate agglutinin gene was cloned into
SJ-10 and WB for expression by a shuttle vector, and YY-11 was mediated by Agrobacterium tumefaciens. Positive transformants
were evaluated using PCR and Western blot assay. Recombinant endophytes colonized most of crops, and resistance of rice
seedlings, which were inoculated with the recombinant endophytic bacteria, to white backed planthoppers was dramatically
enhanced by decreasing...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4862/version/1
Detection strategies for non-authorized GMO products on the European market
Provedor de dados: 17
Autores: Patrick Gürtler; Esther Meissner; Ulrich Busch.
Import and planting of biotech crops is strictly regulated in Europe. In order to observe these strict regulations, an efficient
surveillance of the European market is required. In Europe, data on authorised biotech crops from foreign countries are on hand,
however, only scarce information on non-authorised biotech crops is available. Experience of the last years has shown that
non-authorized biotech crops (e.g. biotech-papaya, Bt10-maize, LL601-rice and Bt63-rice) were detected on the European market.
The Bavarian Health and Food Safety Authority is responsible for the detection of illegal imports and for labelling control of Food
and Feed in Bavaria (Germany). The quantitative real-time PCR is the method of choice for the detection of genetic...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4444/version/1
We Need A Web For Data
Provedor de dados: 17
Autores: John Wilbanks.
The Web has many forums for sharing personal data, but not for scientific data and not in a way that allows the data to be
accessed by "machines as users." A Web of data could add tremendous value by integrating disparate
disciplines or conduct data-driven queries. Doing this is very complex and requires more robust standards than currently exist. The
intended user for most data is not a person; it is a software application that can manipulate the data into something useful for
humans. Such software could be "search engines, analytic software, visualization tools, database back ends, and
more." This need creates a much different requirement for standards than those that were developed for displaying web
data to people....
Tipo: Manuscript
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5499/version/1
Increased regeneration efficiency of _Brassica napus_ L. cultivars Star, Westar and Cyclone from
hypocotyle and cotyledonary explants
Provedor de dados: 17
Autores: Israr Khan; Waqar Ali; Zahir Ali Takar; Arfa Farooqi; Sikandar Khan; Muhammadwaheed Akhtar.
The comparative organogenesis of _Brassica napus_ L cultivars Cyclone, Star and Westar was studied. The cotyledonary explants
gave a higher response to all the combinations of 0.5 mg/L 2,4-D and BAP (0.5, 1.0,1.5 and 2.0 mg/L} used for optimizing the
conditions for callus induction. The best mean weight and mean length of callus was obtained at 0.5 mg/L 2,4-D and 1.5mg/L
BAP for Star cotyledonary explants. For the complete plant regeneration the new method of exposing the explants culture to
Growth regulator free medium was performed. The method was applicable to both hypocotyl and cotyledonary explants. The
Shoot Induction Frequency for hypocotyl (6-34%) in the three cultivars is higher than the cotyledonary explants (3-23%). The
method is speedy and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4781/version/1
Density alteration in non-physiological cells
Provedor de dados: 17
Autores: Yu Liu; Yuan-Chang Zhu; Bin-Bin Wu; Xiao-Hua Wu; Fei-Fei Lan; Jian-Ge Wei; Ming Wang; Hai-Cheng Li; Lin-Na
Li; Ju-Xiang Li; Jia Fei.
In the present study an important phenomenon of cells was discovered: the change of intracellular density in cell's
response to drug and environmental factors. For convenience, this phenomenon is named as "density alteration in
non-physiological cells" ( DANCE). DANCE was determined by discontinuous sucrose gradient centrifugation
(DSGC), in which cells were separated into several bands. The number and position of the bands in DSGC varied with the change
of cell culture conditions, drugs, and physical process, indicating that cell's response to these factors was associated
with alteration of intracellular density. Our results showed that the bands of cells were molecularly different from each other, such
as the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6541/version/1
The History, Development and Impact of Computed Imaging in Neurological Diagnosis and
Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed
diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the
advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities,
forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama
of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/5
Purification and physicochemical studies of metalloprotease, cotinifolin from Euphorbia cotinifolia
Provedor de dados: 17
Autores: Reetesh Kumar.
A detailed procedure for the purification of cotinifolin from the latex of Euphorbia cotinifolia using ion exchange chromatography
and hydrophobic interaction chromatography is described.
Various biochemical characteristics of cotinifolin
and other enzymatic properties along with spectroscopic parameters like circular dichorism and fluorescence have been reported.
Cotinifolin is stable over a wide range of pH and under extreme conditions of chaotrops and organic solvents where most proteins
lost their functions. Further studies on the identification of physiological substrates, biophysical and structural studies will give
insight into the utility of the enzyme in the biotechnological industries and protein engineering.
Tipo: Presentation
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6397/version/1
Cost effective mass multiplication of Gymnema sylvestre in hydroponic system
Provedor de dados: 17
Autores: Raju Karthic; Sundaram Seshadri.
Present experiment was designed with an objective to develop a cost efficient mass multiplication method for Gymnema sylvestre
using hydroponic system. A plastic tub, with polyethylene cover, containing 1/10 strength of MS salts supplemented with Indole
butyric acid (IBA) at different concentrations (0.5, 1.0, 2.5 mg/L) was studied. Medium containing 0.5mg/L of IBA produced
highest rooting (66%) with 96 % survival. This protocol will serve as an alternative to the existing in vitro and clonal
multiplication protocols.
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3770/version/1
Novel approaches, including systems biology, to HIV vaccine research and development: Report from
a Global HIV Vaccine Enterprise Working Group
Provedor de dados: 17
Autores: Bali Pulendran; Rino Rappuoli; Alan Aderem; the Working Group convened by the Global HIV Vaccine Enterprise.
The Global HIV Vaccine Enterprise convened a two-day workshop on August 10-11 2009, at the Fred Hutchison Cancer Research
Center offices in Seattle, WA, to discuss the application of novel approaches,including systems biology, to HIV vaccine research
and development. The goals of this Working Group were to identify key scientific issues and opportunities that have emerged
since the Enterprise Scientific Strategic Plan1 was published in 2005, and to make recommendations to Enterprise stakeholders.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4798/version/2
Cellular delivery of antibodies: effective targeted subcellular imaging and new therapeutic tool
Provedor de dados: 17
Autores: Marzia Massignani; Irene Canton; Nisa Patikarnmonthon; Nicholas J. Warren; Steven P. Armes; Andrew L. Lewis;
Giuseppe Battaglia.
It is already more than a century since the pioneering work of the Nobel Laureate Ehrlich gave birth to the side chain theory1,
which helped to define antibodies and their ability to target specific biological sites. However, the use of antibodies is still
restricted to the extracellular space due to the lack of a suitable delivery vehicle for the efficient transport of antibodies into live
cells without inducing toxicity. In this work, we report the efficient encapsulation and delivery of antibodies into live cells with no
significant loss of cell viability or any deleterious affect on the cell metabolic activity. This delivery system is based on
poly(2-(methacryloyloxy)ethyl phosphorylcholine)-block-(2-(diisopropylamino)ethyl methacrylate), (PMPC-PDPA),...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Immunology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4427/version/1
Development of a Decision Support System (DSS) for an integrated test system towards the effective
and competitive risk assessment on transgenic plants
Provedor de dados: 17
Autores: Christine Höflich; Andreas Müller; Jörg Schmidtke; Kerstin Schmidt; Inge
Broer.
Transgenic plants need specific approaches to analyse their potential impact on environment and consumer. The procedures used
to date are often too extensive and time-consuming and associated with high costs. The BioOK network was established to
develop an effective and competitive risk assessment on transgenic plants based on interdisciplinary research. New and effective
methods for the risk assessment were established which focus on transgene-specific effects measured in in vitro systems in the
laboratory or greenhouse whenever possible. Nevertheless event-specific field experiments have to be carried out in addition to
implement
environmental
effects
e.g.
on
expression.

The...
non-targets
or
the
variability
of
transgene
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4438/version/2
Quality decay and shelf-life study of fresh celery (Apium graveolens L.) grown under different
nitrogen fertilization
Provedor de dados: 17
Autores: Valeria Rizzo; Giuseppe Muratore; Marco Antonio Russo; Riccardo Iacona; Adalgisa Belligno.
Nitrogen fertilization is useful and widespread to obtain higher growth and quality of productions. To evaluate the influence of
nitrogen-rich fertilizers on the quality characteristics, shelf-life tests of celery grown with two levels (80-120 kg ha-1) of two
different nitrogen fertilizers were performed. Celery plants were selected and packed in two plastic films (anti-fog polyolefin and
micro-perforated polypropylene). Nitrate contents, weight loss, hardness, changes in colour parameters and total phenols, were
studied in both packaging solutions during storage. Results showed that celery fertilized with organic nitrogen and packed in
anti-fog polyolefin reached a shelf-life of 37 days. Not treated sample packed in micro-perforated polypropylene was not...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4180/version/1
Polyethylenimine-Modified Multiwalled Carbon Nanotubes for Plasmid DNA Gene Delivery
Provedor de dados: 17
Autores: Bo-Zhang Yu; Ji-Fei Ma; Wen-Xin Li.
An efficient molecular delivery technique based on the transporting high-molecular-weight PEI 600K-modified multiwalled
carbon nanotubes (PEI 600K-MWCNTs) into cell membranes is reported. The PEI 600K-MWCNTs exhibit low cytotoxicity and
its associated plasmid DNA (pDNA) is delivered to cells efficiently, and the green fluorescent protein (GFP) levels up to 18 times
higher than that of naked DNA were observed.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/2753/version/1
Three Dimensional Quantitative Structure-Activity Relationships of Sulfonamides Binding
Monoclonal Antibody by Comparative Molecular Field Analysis
Provedor de dados: 17
Autores: Zhanhui Wang; Xingyuan Cao; Zhenpeng Kai; Ross C. Beier; Congming Wu; Haiyang Jiang; Linli Cheng; Jianzhong
Shen; Xinling Yang.
The three-dimensional quantitative structure-activity relationship (3D-QSAR) model of sulfonamide analogs binding a
monoclonal antibody (MabSMR) produced against sulfamerazine, was carried out by comparative molecular field analysis
(CoMFA). The affinities of MabSMR, expressed as Log10IC50, for 17 sulfonamide analogs were determined by competitive
fluorescence polarization immunoassay (FPIA). Removal of two outliers from the initial set of 17 sulfonamide analogs improved
the predictability of the models. The 3D-QSAR model of 15 sulfonamides resulted in q2cv values of 0.600, and r2 values of
0.995, respectively. This novel study combining FPIA with CoMFA demonstrates that multidisciplinary research can be used as a
useful tool to investigate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2646/version/1
The use of coral as a graft in a large cortical bone defect
Provedor de dados: 17
Autores: Hassan Z. Eskandar; Wan Zulmi; Abdul Hamid Suzina; Abd Rani Samsudin.
The use of coral as space filler in a large cortical bone defect was studied. Twelve Australian White Rabbit were divided into 8
and 12 week group. All rabbits were operated to create a 2.5cm defect in right tibia and replaced with coral; partially covered with
the free autologous non-vascularized periosteum harvested from contralateral tibia. The coral was transfixed and the leg was
immobilized with Plaster of Paris. At the end of 8th and 12th week, all the rabbits were assessed clinically, radiologically and
sacrified for histology evaluation. Bony union achieved in 3 out of 5 rabbits at 8th week while 3 out of 4 at 12th supported by
radiological studies. Histological examination showed healing at coral-cortical junction by callus formation with...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2264/version/1
Modelling Hepatic Endoderm Development: Highly Efficient Differentiation of Human Embryonic
Stem Cells to Functional Hepatic Endoderm Requires ActivinA and Wnt3a Signalling.
Provedor de dados: 17
Autores: David Hay; Judy Fletcher; Catherine Payne; John D. Terrace; Ronald C. J. Gallagher; Jan Snoeys; Jim Black; Davina
Wojtacha; Kay Samuel; Zara Hannoun; Anne Pryde; Celine Filippi; Ian S. Currie; Stuart J. Forbes; James A. Ross; Philip
Newsome; John Iredale.
Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. However, their homogeneous cellular
differentiation to specific cell types _in vitro_ has proven difficult thus far. Wnt signalling has been shown to play important roles
in coordinating development and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver
development _in vivo_. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our _in vitro_
model, demonstrating the importance of a physiological approach to cellular differentiation. Our studies provide compelling
evidence that Wnt3a signaling is important for coordinated hepato-cellular function _in vitro_ and _in vivo_. In addition, we
demonstrate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1454/version/1
Consensus virtual screening approaches to predict protein ligands
Provedor de dados: 17
Autores: Andreas Kukol.
In order to exploit the advantages of receptor-based virtual screening, namely time/cost saving and specificity, it is important to
rely on algorithms that predict a high number of active ligands at the top ranks of a small molecule database. Towards that goal
consensus methods combining the results of several docking algorithms were developed and compared against the individual
algorithms. Furthermore, a recently proposed rescoring method based on drug efficiency indices was evaluated. Among AutoDock
Vina 1.0, AutoDock 4.2 and GemDock, AutoDock Vina was the best performing single method in predicting high affinity ligands
from a database of known ligands and decoys. The rescoring of predicted binding energies with the water/butanol partition
coeffcient...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5979/version/1
Prediction of Evolutionarily important catalytic amino acid of Mycobacterium tuberculosis
O-Succinylbenzoate synthase through in silico mutational analysis
Provedor de dados: 17
Autores: Babajan B; Chaitanya M; Anuradha CM; Suresh Kumar Chitta.
The emergence of tuberculosis resistant to multiple, first- and second-line antibiotics poses challenges to a global control strategy
that relies on standard drug treatment regimens. The high drug-resistant strains of Mycobacterium tuberculosis (Mtb) have been
implicated in outbreaks and have been found throughout the world; a comprehensive understanding, the magnitude of this threat
requires an accurate assessment of the worldwide burden of resistance. In an attempt to design anti-TB drugs, the target chosen is
a key enzyme of Mtb, O-Succinylbenzoate synthase (OSBS), which is an attractive target for its role in electron transport chain as
OSBS is not available in humans. An attempt has been to built the 3-D structure of Mtb-OSBS using online Swiss model...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3776/version/1
X-ray phase contrast imaging of biological specimens with tabletop synchrotron radiation
Provedor de dados: 17
Autores: Stefan Kneip; Chris McGuffey; Franklin Dollar; Michael S. Bloom; Vladimir Chvykov; Galina Kalintchenko; Karl
Krushelnick; Anatoly Maksimchuk; Stuart P. D. Mangles; Takeshi Matsuoka; Zulfikar Najmudin; Charlotte A. J. Palmer; Joerg
Schreiber; Will Schumaker; Alexander G. R. Thomas; Victor Yanovsky.
Since their discovery in 1896, x-rays have had a profound impact on science, medicine and technology. Here we show that the
x-rays from a novel tabletop source of bright coherent synchrotron radiation can be applied to phase contrast imaging of biological
specimens, yielding superior image quality and avoiding the need for scarce or expensive conventional sources.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/5946/version/1
Post-market monitoring: legal framework in Brazil and first results
Provedor de dados: 17
Autores: Marcia A. Melo; Ederson A. Kido; Paulo P. Andrade.
Brazil is presently (2010) the second largest producer of Genetically Modified (GM) crops. The legal framework to support both
research activities and the commercial release of Genetically Modified Organisms (GMOs) is, however, very recent, as the
Biosafety Law dates back from 2005. After the indispensable risk analysis, done by National Technical Committee for Biosafety
(CTNBio), a GM plant can be approved for commercial release. Nevertheless, as stated in the Normative Resolution nr. 5 (NR-5),
2008, the post-market monitoring is mandatory. However, the first GM crop in Brazil, the herbicide tolerant soybean, was
harvested in 2005. The CTNBio asked, then, for a monitoring plan and the applicant designed a complex study focused on
case-specific monitoring....
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4528/version/1
ESG: Extended Similarity Group method for automated protein function prediction
Provedor de dados: 17
Autores: Meghana Chitale; Troy Hawkins; Changsoon Park; Daisuke Kihara.
We present here the Extended Similarity Group (ESG) method, which annotates query sequences with Gene Ontology (GO) terms
by assigning probability to each annotation computed based on iterative PSI-BLAST searches. Conventionally sequence
homology based function annotation methods, such as BLAST, retrieve function information from top hits with a significant score
(E-values). In contrast, the PFP method, which we have presented previously, goes one step ahead in utilizing a PSI-BLAST result
by considering very weak hits even an E-value of up to 100 and also by incorporating the functional association between GO
terms (FAM matrix) computed using term co-occurrence frequencies in the UniProt database. PFP is very successful which is
evidenced by the top rank...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2193/version/1
Improving cellular cancer vaccines
Provedor de dados: 17
Autores: Petr Lokhov.
Immunization with cancer cells is of great demand in anti-cancer therapy. However, current cellular vaccines are inefficient and
there are questions regarding their overall safety. We report a simple and straightforward approach for improving of cellular
cancer vaccines. Through treatment of cancer cell cultures with purified protease, it is possible to make preparations of
cell-surface antigens that are free of intracellular content and contain two orders-of-magnitude less protein than the whole lysate
of an equivalent number of cancer cells. Despite this difference in total protein content, protease-generated preparations stimulate
anti-cancer responses from immune cells better those stimulated with cancer cells themselves. The composition of collected...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4356/version/1
Optimal control circuitry design for the digital p53 dynamics in cancer cell and apoptosis
Provedor de dados: 17
Autores: Rosario M. Ardito Marretta.
Experimental work and theoretical models deduce a "digital" response of the p53 transcription factor when
genomic integrity is damaged. The mutual influence of p53 and its antagonist, the Mdm2 oncogene, is closed in feedback. This
paper proposes an aerospace architecture for translating the p53/Mdm2/DNA damage network into a digital circuitry in which the
optimal control theory is applied for obtaining the requested dynamic evolutions of some considered cell species for repairing a
DNA damage. The purpose of this paper is not to improve the analysis of the actual mathematical models but to demonstrate the
usefulness of such digital circuitry design capable to predict and detect the cell species dynamics for finding more information...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2870/version/1
Production of Transgenic Cloned Miniature Pigs with Membrane-bound Human Fas Ligand (FasL)
by Somatic Cell Nuclear Transfer
Provedor de dados: 17
Autores: Jae Goo Seol; Soo Hyun Kim; Dong-Il Jin; Seung Pyo Hong; Ji Yeon Yoo; Ki Myung Choi; Young Chul Park; Yun Jin
Yun; Kwang Wook Park; Jae Young Heo.
Cell-mediated xenograft rejection, including NK cells and CD8+ CTL, is a major obstacle in successful pig-to-human
xenotransplantation. Human CD8+ CTL and NK cells display high cytotoxicity for pig cells, mediated at least in part by the
Fas/FasL pathway. To prevent cell-mediated xenocytotoxicity, a membrane-bound form of human FasL (mFasL) was generated as
an inhibitor for CTL and NK cell cytotoxicity that could not be cleaved by metalloproteinase to produce putative soluble FasL.
We produced two healthy transgenic pigs harboring the mFasL gene via somatic cell nuclear transfer (SCNT). In a cytotoxicity
assay using transgenic clonal cell lines and transgenic pig ear cells, the rate of CD8+ CTL-mediated cytotoxicity was significantly
reduced in transgenic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology.
Ano: 2010
URL: http://precedings.nature.com/documents/4539/version/1
The Human Virome in Children and its Relationship to Febrile Illness
Provedor de dados: 17
Autores: Gregory A. Storch; George M. Weinstock; Erica J. Sodergren; Kristine M. Wylie; Max Q. Arens; Richard S. Buller;
David M. Jaffe; Jared T. Muenzer.
This study investigates the relationship of viruses to febrile illness in children. Subjects are normal children 2-36 months of age
with fever along with normal children of the same age without fever, plus immunocompromised children with fever along with
immunocompromised children without fever.
Specimens obtained include blood, nasopharyngeal secretions, and feces.
Specimens are analyzed using a panel of virus-specific PCR assays and also by high throughput sequencing using 454 and
Illumina platforms.
Tipo: Marker Paper / Data Plan
Palavras-chave: Biotechnology; Microbiology; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5319/version/1
Structural Analysis of Outer Membrane Beta-stranded Porins using Temperature Factor
Provedor de dados: 17
Autores: Abhishek Kumar.
Computational and statistical analysis has formed a large component of the biophysical efforts put forth to understand protein
structure and function, due to the diversity and complexity of their structure. Outer membrane proteins form a diverse and
complex set of proteins. Of these, porins which allow passage of molecules across the membrane interface have been analyzed
here from a biophysical and structural perspective. The objective of this study is to analyze the structural organization of porins
using atomic temperature factor as a parameter. Generally atomic temperature factors of molecules from crystal structures indicate
the degree of mobility or disorder seen in the crystal structure. As good crystal structures have fewer possibilities of errors...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7016/version/1
Glutathione (GSH) conjugates with dopamine (DA)-derived quinones to form reactive or non-reactive
GSH-conjugates
Provedor de dados: 17
Autores: Zhidong Zhou; Tit Meng Lim.
In this study we demonstrate for the first time that GSH could rapidly conjugate with dopamine (DA)-derived DA-o-quinones
without
enzymatic
catalysis
to
form
short-lived
intermediate
GSH-conjugates
(2-S-GSH-DA-o-quinone
and
5-S-GSH-DA-o-quinone). These intermediate GSH-conjugates are unstable and would finally form reactive or non-reactive
GSH-conjugates dependent on ambient reductive forces. Under insufficient reductive forces, the intermediate GSH-conjugates
could cyclize spontaneously to form reactive 7-S-GSH-aminochrome (7-S-GSH-AM). The 7-S-GSH-AM is so reactive that it
could further react with another GSH to form 4,7-bi-GSH-5,6-dihydroindole. Its reactivity could also abrogate tyrosinase activity
in solutions. In addition, the 7-S-GSH-AM could...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/2963/version/1
MRI and MRSI Application in Neurodegeneration
Provedor de dados: 17
Autores: Rakesh Sharma.
MRI combined with MR spectroscopy generates better information of both brain tissue composition and mesurement of
metabolites or neurochemicals in multiple sclerosis(MS), Alzheimer's disease and epilepsy. New fast imaging
techniques are technical advance in visualizing accurate anatomy and measurement of absolute neurochemicals in brain. Gray
metter seated MS lesions is a new form of disease and lesion may appear normal on MRI but show spectroscopy peaks. High
magnetic field scanners are available for fast imaging.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4317/version/1
Computational binding mechanism of Mycobacterium tuberculosis UDP-NAG enolpyruvyl
transferase (MurA) with inhibitors fosfomycin, cyclic disulfide analog RWJ-3981, pyrazolopyrimidine
analog RWJ-110192, purine analog RWJ-140998, 5-sulfonoxy-anthranilic aci
Provedor de dados: 17
Autores: C. M. Anuradha; B. Babajan; M. Chaitanaya; C. Rajasekhar; Suresh Kumar Chitta.
Worldwide, tuberculosis (TB) remains the most frequent and important infectious disease causing morbidity and death. One-third
of the world's population is infected with Mycobacterium tuberculosis (Mtb), the etiologic agent of TB. In this
context, TB is in the top three, with malaria and HIV being the leading causes of death from a single infectious agent, and about
two million deaths are attributable to TB annually. The bacterial enzyme MurA catalyzes the transfer of enolpyruvate from
phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG), which is the first committed step of bacterial
cell wall biosynthesis. In this work, 3D structural model of Mtb-MurA enzyme has been developed, for the first time, by
homology modeling and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3731/version/1
Mass Cultivation of _Chroococcus turgidus_ and _Oscillatoria sp._ and Effective Harvesting of
Biomass by Low-cost methods
Provedor de dados: 17
Autores: Mohan N; Hanumantha Rao P; Ranjith Kumar R; Sivasubramanian V.
The potential of microalgal biomasses for the production of biochemicals such as pigments, vitamins, antibiotics, polysaccharides,
proteins, essential fatty acids, bio-flocculants, enzymes, etc. is well known. The purpose of this study was to cultivate two
cyanobacterial species namely _Chroococcus turgidus_ and _Oscillatoria sp._, isolated from industrial effluents, using a suitable
growth medium in a large-scale High Rate Algal (HRA) pond. The biomolecules such as total protein, total carbohydrate and total
lipid, and the pigments chlorophyll, phycobilins, β-carotene, were analysed at regular intervals during cultivation. In
addition, the total bacterial cell numbers were enumerated during the study and their influence on algal growth was...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4331/version/1
Quantification of Cell Movement Reveals Distinct Edge Motility Types During Cell Spreading
Provedor de dados: 17
Autores: Benjamin J. Dubin-Thaler; Jake M. Hofman; Harry V. Xenias; Ingrid A. Spielman; Anna H. Shneidman; Lawrence
David; Hans-Guenther Dobereiner; Chris Wiggins; Michael P. Sheetz.
Actin-based motility is central to cellular processes such as migration, bacterial engulfment, and cancer metastasis, and requires
precise spatial and temporal regulation of the cytoskeleton. We studied one such process, fibroblast spreading, which involves
three temporal phases: early, middle, and late spreading, distinguished by differences in cell area growth. In these studies, aided
by improved algorithms for analyzing edge movement, we observed that each phase was dominated by a single, kinematically and
biochemically distinct cytoskeletal organization, or motility type. Specifically, early spreading was dominated by periodic
blebbing; continuous protrusion occurred predominantly during middle spreading; and periodic contractions were prevalent in...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/391/version/1
MMsPred: a bioactivity and toxicology predictive system
Provedor de dados: 17
Autores: Joel Masciocchi; Luca Pireddu; Ricardo Medda; Piergiorgio Palla; Matteo Floris; Luca Pani; Patricia Rodriguez-Tome.
In the last decade, the development and use of new methods in combinatorial chemistry and high-throughput screening has
dramatically increased the number of known biologically active compounds. Paradoxically, the number of drugs reaching the
market has not followed the same trend, often because many of the candidate drugs present poor qualities in absorption,
distribution, metabolism, excretion, and toxicological properties (ADME-Tox). The ability to recognize and discard bad
candidates early in the drug discovery steps would save lost investments in time and money. Machine learning techniques could
provide solutions to this problem.
The goal of my research is to develop classifiers that accurately
discriminate between active and inactive...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4188/version/1
Changes in Antioxidant Enzymes Activity and Oxidative Damage in Four Argania spinosa Ecotypes
Under Water Stress Conditions
Provedor de dados: 17
Autores: Abdelghani Chakhchar; Abderrahim Ferradous; Mouna
Lamaoui; Said Wahbi; Cherkaoui El Modafar.
_Argania spinosa_ L. Skell, a tree endemic of Morocco, grows in semiarid and arid areas. Biochemical response to drought in four
ecotypes of the _A. spinosa_ was compared. Choice of ecotypes was based on evident differences in geographical features in
south-west Morocco: two coastal ecotypes (Essaouira and Agadir) and two paralittorale ecotypes (Aoulouz and Lakhssas). Lipid
peroxidation (malonyldialdehyde (MDA)), content of endogenous hydrogen peroxide (H2O2) and antioxidant enzyme system
(Catalase (CAT), Ascorbate peroxidase (APX), Total peroxidase (POD) and Polyphenoloxidase (PPO) were measured in control
(100% relative humidity (RH) at field capacity (FC)) and water-stressed plants (50 and 25% RH at FC). For all ecotypes, drought
stress significantly...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6189/version/1
Design of selective peptide antibiotics by using the sequence moment concept
Provedor de dados: 17
Autores: Davor Juretic; Nada Ilic; Damir Vukicevic; Nikolinka Antcheva; Alessandro Tossi.
Withdrawn

Incomplete data
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3068/version/2
MIREOT: the Minimum Information to Reference an External Ontology Term
Provedor de dados: 17
Autores: Melanie Courtot; Frank Gibson; Allyson L. Lister; James Malone; Daniel Schober; Ryan R. Brinkman; Alan
Ruttenberg.
While the Web Ontology Language (OWL) provides a mechanism to import ontologies, this mechanism is not always suitable.
First, given the current state of editing tools and the issues they have working with large ontologies, direct OWL imports have
sometimes proven impractical for day-to-day development. Second, ontologies chosen for integration may be under active
development and not aligned with the chosen design principles. Importing heterogeneous ontologies in their entirety may lead to
inconsistencies or unintended inferences. In this paper we propose a set of guidelines for importing required terms from an
external resource into a target ontology. We describe the guidelines, their implementation, present some examples of application,
and outline...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2009
URL: http://precedings.nature.com/documents/3574/version/1
Open Standards and Resources in Systems Biology: collaborative scale-up toward virtual life
Provedor de dados: 17
Autores: Nicolas Le Novere.
The practise of Systems Biology relies on interfaces. Interfaces
between the entities we study: the paradigm
moved
from
a
physical
object
centric
view
toward
a
relationship-centric
one;
interfaces
between tools: From the retrieval of the primary data to the fine
analysis of
a model's behaviour, one uses many tools, more or less
well connected; interfaces between
individuals:
To
build
any
non-trivial
mechanistic
model
requires
to
merge
existing
work
and
gather external expertise.

If we want these interfaces to be
generic enough to allow for anybody
to leverage on existing...
Tipo: Presentation
Palavras-chave: Biotechnology; Data Standards.
Ano: 2006
URL: http://precedings.nature.com/documents/10/version/1
Dual expression recombinase based (DERB) single vector system for high throughput screening and
verification of protein interactions in living cells
Provedor de dados: 17
Autores: Jian-Ping Lu; Laura Beatty; Jehonathan Pinthus.
Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary
for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial,
however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of
protein interactions in living cells. The platform’s series of Dual Expression Recombinase Based (DERB) destiny
vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of
interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1550/version/2
New fungal sources for α-L-Rhamnosidase: an important enzyme used in the synthesis of
drugs and drug precursors
Provedor de dados: 17
Autores: Vinita Yadav; Kapil Deo Singh Yadav.
Two fungal strains were isolated and tentatively identified as Penicillium VY and Aspergillus VY. All the isolated species show
the maximum production on third day in a liquid culture media. The pH optimum was found to be 10.0 for Penicillium VY and
11.0 for Aspergillus VY. The temperature optima were 50ºC in both the cases. The enzyme produced by Penicillium
VY was found to be stable in the pH range 3.0-7.0 and 3.0–6.0 in case of Aspergillus VY. The enzyme does not
loose activity up to 40º C in case of Penicillium VY and 40ºC in case of Aspergillus VY if exposed for 1
h.

Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2560/version/1
A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with
Unprecedented Stereoselectivity
Provedor de dados: 17
Autores: Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for
the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties
and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate
ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a
unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia
coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/1697/version/1
Getting The Most Out of Your Research
Provedor de dados: 17
Autores: Folarin A. Erogbogbo.
Research is the cornerstone of invention, facilitator of innovation, and mother of discovery. Even though many students are
competent in advancing through the class room and getting good grades, excellent research requires a different set of attitudinal
and academic skills. This workshop, "Get the most out of your research" is an outstanding program
developed from extensive time spent with brilliant scientists and a wide variety of mentored students. Familiarize yourself with
the fine points of this workshop and arm yourself for a successful research career.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/5702/version/1
SABIO-RK: Access to Biochemical Reaction Kinetics Data
Provedor de dados: 17
Autores: Martin Golebiewski.
SABIO-RK (http://sabio.h-its.org/) is a curated database for biochemical reaction kinetics data drawn from different sources: from
literature or direct submission by experimenters. The system offers standardized data by the use of controlled vocabularies and
annotations pointing to other resources and biological ontologies. SABIO-RK can be accessed either manually via a web-based
search interface or automatically via web services that allow direct data access by other tools. Both interfaces support the export
of the data together with its annotations in SBML (Systems Biology Markup Language) complying with the MIRIAM (Minimal
Information Requested In the Annotation of biochemical Models) standard. New RESTful web services, as well as a new
browser...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6506/version/1
Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense
oligonucleotide
Provedor de dados: 17
Autores: Xiaolong Wang; Deming Gou.
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to
scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process. Here we report a novel
thermocyclic reaction, polymerase-endonuclease amplification reaction (PEAR), for the amplification of oligonucleotides. A
target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation
and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and
thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides. Each round of PEAR achieves
>100-fold...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/3711/version/1
Universality of a mesenchymal transition signature in invasive solid cancers
Provedor de dados: 17
Autores: Dimitris Anastassiou.
In this brief communication, additional computational validation is provided consistent with the unifying hypothesis that a shared
biological mechanism of mesenchymal transition, reflected by a precise gene expression signature, may be present in all types of
solid cancers when they reach a particular stage of invasiveness.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6862/version/1
Identity in research infrastructure and scientific communication: Report from the 1st IRISC
workshop, Helsinki Sep 12-13, 2011
Provedor de dados: 17
Autores: Gudmundur A. Thorisson; Mikael Linden; Anthony J. Brookes; Myles Byrne; Juha Muilu; Tommi Nyronen.
Motivation for the IRISC workshop came from the observation that identity and digital identification are increasingly important
factors in modern scientific research, especially with the now near-ubiquitous use of the Internet as a global medium for
dissemination and debate of scientific knowledge and data, and as a platform for scientific collaborations and large-scale e-science
activities.

The 1 1/2 day IRISC2011 workshop sought to explore a series of
interrelated topics under two main themes: i) unambiguously identifying authors/creators & attributing their scholarly
works, and ii) individual identification and access management in the context of identity federations. Specific aims of the
workshop...
Tipo: Manuscript
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6609/version/1
In vitro shoot regeneration from cotyledon of redgram
Provedor de dados: 17
Autores: Telugu Raghavendra; Palagiri Sudhakar; K Balakrishna Reddy; Keerthi Venkaiah; Putta Latha; Tollamadugu NVKV
Prasad; Lakkireddi Prasanthi.
Pigeonpea is one of the most popular legume grains in the world, especially in the Indian sub-continent. Keeping in view the
importance of the crop, an efficient protocol for rapid in vitro plant regeneration from cotyledon of redgram has been reported in
this paper. The concentraion of the hormones played an important role in shoot and root regeneration.
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7041/version/1
Hierarchical nanomechanics of collagen microfibrils
Provedor de dados: 17
Autores: Alfonso Gautieri; Simone Vesentini; Alberto Redaelli; Markus J. Buehler.
Collagen constitutes one third of the human proteome, providing mechanical stability, elasticity and strength to connective tissues.
Collagen is also the dominating material in the extracellular matrix (ECM) and is thus crucial for cell differentiation, growth and
pathology. However, fundamental questions remain with respect to the origin of the unique mechanical properties of collagenous
tissues, and in particular its stiffness, extensibility and nonlinear mechanical response. By using x-ray diffraction data of a
collagen fibril reported by Orgel et al. (Proceedings of the National Academy of Sciences USA, 2006) in combination with protein
structure identification methods, here we present an experimentally validated model of the nanomechanics of a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4995/version/1
The Mass-Action-Law Based GPS Concept for Bio-Informatics
Provedor de dados: 17
Autores: Ting-Chao Chou.
The unified theory of dose and effect, as indicated by the median-effect equation for single and multiple entities and for the first
and higher order kinetic/dynamic, has been established by T.C. Chou and it is based on the physical/chemical principle of the
mass-action law (_J. Theor. Biol._ 59: 253-276, 1976; _Pharmacological Rev._ 58: 621-681, 2006). Rearrangements of the
median-effect
equation
lead
to
“median”
Michaelis-Menten,
serves
as
the
Hill,
Scatchard,
and
“universal
Henderson-Hasselbalch
reference
equations.
point”
and
The
the
“common link” for the relationship of all entities at all dynamic orders, and is also the
“harmonic mean” of kinetic dissociation...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2064/version/1
Effect of Stimulating Magenetic Field on Plants
Provedor de dados: 17
Autores: R. V. K. Charan.
A plant's metabolism contains 90-95 percent of diamagnetic water and rest contains several apra, ferro and dia metals
and non-metals.The present work is to study stimulating magnetic effect on plants. In result I find abrupt rising of the pulse rate
which may enhance the growth of the plant resulting in removing of necessity of using fertilizers and chemicals for plant growth if
it is for human consumption. A relation can also be established in between plant pulsation and the applied field.
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3300/version/1
Tales From the Code Front: Translating Modularity
Provedor de dados: 17
Autores: Lucian P. Smith.
In translating modular Antimony models to and from CellML, two differences appeared:
CellML's
'black box' approach vs. Antimony's 'model composition' approach,
and CellML's math-based encoding vs. Antimony's object and rule-based encoding. The latter turned out
to have a much larger impact on the translation than anticipated.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5132/version/1
Predicting Prostate Cancer Aggressiveness through a Nanoparticle Test
Provedor de dados: 17
Autores: Qun Huo; Shannon Sullivan; Hillari Hallquist; Sally Litherland; David Decker; Inoel Rivera-Ramirez.
Prostate cancer (PCa) is the most common malignancy and the second leading cause of cancer death in American men. Due to the
lack of accurate tests to distinguish aggressive cancer from indolent tumor, prostate cancer is often over-treated. Post-surgery
pathology analysis revealed that 30% of tumors removed by radical prostatectomy are deemed clinically insignificant and would
not have required such invasive treatment.^1^ Over-diagnosis and treatment of low-risk prostate cancer has serious and
long-lasting side effect: as high as 70% of the patients who receive radical prostatectomy treatment will suffer a loss of sexual
potency that cannot be remedied by drugs such as sildenafil citrate.^2^ We herein report a simple nanoparticle-serum protein
adsorption...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6409/version/1
Nano-enabled Biological Materials
Provedor de dados: 17
Autores: Bradly J. Alicea.
This talk is an attempt to define a new field called “Nano-enabled Biological Tissues”. As such, this
talk
serves
as
a
review
of
both


The
the
theoretical
presentation
underpinnings
is
divided
and
relevant
into
recent
several
results.
parts:


* in the first section (slides 3-4), the concept of nano-enabled tissues are introduced
as a complex system that can be engineered at multiple scales.

* the second section
(slides 5-12) contains three essential ingredients to achieve the technological vision. Current examples of each ingredient are
introduced separately.

* in the third section (slides 13-17),...
Tipo: Presentation
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5448/version/1
Systems Pharmacology
Provedor de dados: 17
Autores: Ravi Iyengar.
The slides are from a presentation given by Professor Ravi Iyengar from Mount Sinai School of Medicine at the Drug Forum
Meeting #9 that took place in Washington, DC on February 20-21, 2008. The slides describe two projects: one that was published
last year, and the other unpublished. These projects used network analysis to explore the relationships between FDA approved
drugs and a human protein-protein interaction network.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1749/version/1
Modelling Hepatic Endoderm Development: Highly Efficient Differentiation of Human Embryonic
Stem Cells to Functional Hepatic Endoderm Requires ActivinA and Wnt3a Signalling.
Provedor de dados: 17
Autores: David C. Hay; Judy Fletcher; Catherine Payne; John D. Terrace; Ronald C. J. Gallagher; Jan Snoeys; Jim Black; Davina
Wojtacha; Kay Samuel; Zara Hannoun; Anne Pryde; Celine Filippi; Ian S. Currie; Stuart J. Forbes; James A. Ross; Philip
Newsome; John P. Iredale.
Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. However, their homogeneous cellular
differentiation to specific cell types _in vitro_ has proven difficult thus far. Wnt signalling has been shown to play important roles
in coordinating development and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver
development _in vivo_. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our _in vitro_
model, demonstrating the importance of a physiological approach to cellular differentiation. Our studies provide compelling
evidence that Wnt3a signaling is important for coordinated hepato-cellular function _in vitro_ and _in vivo_. In addition, we
demonstrate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1454/version/2
Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy
Provedor de dados: 17
Autores: Jiehua Zhou; Haitang Li; Shirley Li; John Zaia; John Rossi.
The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific
cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The
envelope glycoprotein is
expressed on the surface of HIV-1 infected cells, allowing binding and interalization of the
aptamer-siRNA chimeric molecules. We demonstrate that the anti-gp120 aptamer-siRNA chimera is specifically taken up by cells
expressing HIV-1 gp120, and the appended siRNA is processed by Dicer, releasing an anti-tat/rev siRNA which in turn inhibits
HIV replication. We show for the first time a dual functioning aptamer-siRNA chimera in which both the aptamer and the siRNA
portions have...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1299/version/1
An Olfactory Receptor Pseudogene whose Function emerged in Humans
Provedor de dados: 17
Autores: Peter Lai; Gautam Bahl; Maryse Gremigni; Valery Matarazzo; Olivier Clot-Faybesse; Catherine Ronin; Chiquito J.
Crasto.
Human olfactory receptor, hOR17-210, is identified as a pseudogene in the human genome. Experimental data has shown
however, that the gene product of cloned hOR17-210 cDNA was able to bind an odorant-binding protein and is narrowly tuned for
excitation by cyclic ketones. Supported by experimental results, we used the bioinformatics methods of sequence analysis,
computational protein modeling and docking, to show that functionality in this receptor is retained due to sequence-structure
features not previously observed in mammalian ORs. This receptor does not possess the first two transmembrane helical domains
(of seven typically seen in GPCRs). It however, possesses an additional TM that has not been observed in other human olfactory
receptors. By...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1290/version/1
SNIPER SHOT PEGylation: TGase mediated site-specific conjugation of PEG to proteins
Provedor de dados: 17
Autores: Abhijeet Satwekar.
Commercially available recombinant protein drugs often cause immune reactions in the body which reduces their efficiency.
Protein drugs can be PEGylated, (attachment of polyethylene glycol) to overcome this problem. PEGylation increases
bioavailability by reduced immune reactions and decreased renal clearance [1]. So far the traditional approaches for PEGylation
involve harsh reaction conditions which provide a heterogeneous product (PEG attached randomly to different sites) along with
the formation of several byproducts. Due to heterogeneity, the PEGylated protein drug faces challenge for FDA approval.
Therefore, there is an immense need to develop an approach which could generate a homogeneous PEGylated protein drug.

The...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/5671/version/1
Hierarchical nanomechanics of collagen microfibrils
Provedor de dados: 17
Autores: Alfonso Gautieri; Simone Vesentini; Alberto Redaelli; Markus J. Buehler.
Collagen constitutes one third of the human proteome, providing mechanical stability, elasticity and strength to organisms and is
thus the prime construction materials in biology. Collagen is also the dominating material in the extracellular matrix and its
stiffness controls cell differentiation, growth and pathology. However, the origin of the unique mechanical properties of
collagenous tissues, and in particular its stiffness, extensibility and nonlinear mechanical response remains unknown. By using
x-ray diffraction data of a collagen fibril (Orgel et al., PNAS, 2006) here we present an experimentally validated model of the
nanomechanics of a collagen microfibril that incorporates the full biochemical details of the amino acid sequence of constituting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4995/version/2
Systems Biology Graphical Notation: Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas Le Novere; Emek Demir; Huaiyu Mi; Alice Villeger.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without
electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialized
notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields.
With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions
of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few
detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/3721/version/4
Long-term Benefits of GM crops: Potential for Diabrotica Suppression in Europe using Bt Maize
Provedor de dados: 17
Autores: Nicholas P. Storer; Peter Schlotter; Kevin L. Steffey.
Transgenic crops producing insecticidal proteins from _Bacillus thuringiensis_ (Bt) have been widely adopted since 1996 in the
United States of America to combat important pests of maize and cotton. There is growing evidence that several target pest
populations have been dramatically reduced in areas where the Bt crops have been most intensively adopted over multiple years.
The evidence is most dramatic for nonmigratory monophagous and oligophagous species that show high mortality on Bt crops,
such as European corn borer (_Ostrinia nubilalis_), and tobacco budworm (_Heliothis virescens_). Bt cotton is currently being
used in the southwestern USA as part of an area-wide eradication program for pink bollworm (_Pectinophora gossypiella_).
Single, stacked, and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4447/version/1
Quantitative MRI and MR Spectroscopy of Multiple Sclerosis, Alzheimer’s Disease,
Epilepsy and Cancer Microimaging Techniques
Provedor de dados: 17
Autores: Rakesh Sharma.
Clinical MRI and MRS have promise in molecular imaging. Microimaging techniques offer a promising research tool to get
noninvasive data in monitoring the effect of drugs.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3485/version/1
Robustness of self-organised systems to changes in individual level behaviour: an example from real
and simulated self-organised snail aggregations
Provedor de dados: 17
Autores: Richard Stafford; Mark S. Davies; Gray A. Williams.
Perfect behaviours that are optimal to the environment an agent operates within rarely exist in real animals or in robotic systems.
The costs (be they biological or economic) of building sensors and processing the information they capture become excessive
compared to the small advantages that occur from the modifications of behaviour. Many self-organised systems are thought to
change their properties as a result of changes in individual behaviour. Here, using both natural systems and computer simulations,
we demonstrate that intertidal snail aggregations slightly decrease in size when individuals forage for shorter periods due to hotter
and more desiccating conditions – a non-optimal behaviour for the snails since aggregation reduces desiccation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3922/version/1
Novel approaches, including systems biology, to HIV vaccine research and development: Report from
a Global HIV Vaccine Enterprise Working Group
Provedor de dados: 17
Autores: Bali Pulendran; Rino Rappuoli; Alan Aderem; the Working Group convened by the Global HIV Vaccine Enterprise.
The Global HIV Vaccine Enterprise convened a two-day workshop on August 10-11 2009, at the Fred Hutchison Cancer Research
Center offices in Seattle, WA, to discuss the application of novel approaches,including systems biology, to HIV vaccine research
and development. The goals of this Working Group were to identify key scientific issues and opportunities that have emerged
since the Enterprise Scientific Strategic Plan1 was published in 2005, and to make recommendations to Enterprise stakeholders.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4798/version/1
Critical Concentration Gradient Model of Bacterial Cell Division
Provedor de dados: 17
Autores: Aijaz A. Rashid; Perminder P. Sing.
Critical concentration gradient model is helpful in understanding the mechanism of Z-ring formation. The distribution of positive
and negative regulators of Z-ring formation in cell is not random but well programmed. The positive and negative regulators in
cell lead to a critical concentration gradient from mid cell to the poles. The critical concentration of FtsZ required for Z-ring
assembly at the centre of cell is less than that at the poles. So FtsZ starts polymerizing at cell centre and afterwards by cooperative
association of more and more monomers leads to the Z-ring formation. Simultaneously during Z-ring formation the recruitment of
other accessory proteins takes place, leading to the formation of a stable divisome complex.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4659/version/1
General updates about SBML and SBML Team activities
Provedor de dados: 17
Autores: Michael Hucka.
In this presentation, I summarize very briefly some recent updates to SBML.org, SBML development, and software from the
SBML Team.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6345/version/1
Allergological and toxicological risk assessment of genetically modified (GM) plants - Development of
a model system
Provedor de dados: 17
Autores: Alain Steinmann; Kristin Entzian; Birgit Grümmer; Udo Meyer.
Technical advances in biotechnology lead to a rising number of genetically modified plants and derived food and feed. Therefore,
the international interest in adequate and standardized analytical methods increases to assess health risks in a rapid and low cost
manner.
To address this issue a decision support system (DSS), which will be based on baseline data and
thresholds of key parameters, is going to be established in the framework of a joint project (BioOK). Determination of the
potential allergenicity or toxicity of novel proteins caused by the genetic modification compared to near isogenic variants (NIV) is
a key issue in this context. For the development of DSS the model plant “potato” was used to
compare...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4441/version/1
Amino acid features: a missing compartment of prediction of protein function
Provedor de dados: 17
Autores: Esmaeil Ebrahimie; Mansour Ebrahimi; Mahdi Ebrahimi.
Enormous computational efforts have been carried out to predict structure and function of protein. However, nearly all of these
efforts have been focused on prediction of function based on primary nucleic acid sequence or modeling 3D structure of protein
from its nucleic acid sequence. In fact, it seems that amino acid attributes, which is an intermediate phase between DNA/RNA and
advanced protein structure, has been missed.
From 2010, we examined the possibility of precise prediction
of structural protein function based on amino acid features by improving the following three aspects of amino acid research: (1)
Increasing the number of computationally calculated amino acid features, (2) Testing different feature selection (attribute...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6693/version/1
The Mass-Action-Law Based GPS Concept for Bio-Informatics
Provedor de dados: 17
Autores: Ting-Chao Chou.
The unified theory of dose and effect, as indicated by the median-effect equation for single and multiple entities and for the first
and higher order kinetic/dynamic, has been established by T.C. Chou and it is based on the physical/chemical principle of the
mass-action law (_J. Theor. Biol._ 59: 253-276, 1976; _Pharmacological Rev._ 58: 621-681, 2006). Rearrangements of the
median-effect
equation
lead
to
“median”
Michaelis-Menten,
serves
as
the
Hill,
Scatchard,
and
“universal
Henderson-Hasselbalch
reference
equations.
point”
and
The
the
“common link” for the relationship of all entities at all dynamic orders, and is also the
“harmonic mean” of kinetic dissociation...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2064/version/2
Copper induced peroxidase, CCPP form Caribbean copper plant
Provedor de dados: 17
Autores: Reetesh Kumar.
Plant peroxidase exhibit broad substrate specificity and is active in a wide range of pH and temperature, and in the presence of
organic compounds as well as other additives. The peroxidase is known to be involved in many physiological and regulatory
processes in plants. This ppt presents an introduction on plant peroxidase and their applications. A purification protocol has been
developed and the purified protein, named as CCPP is biochemically and physiologically characterized. The physiochemical
properties such as molecular weight, isoelectric point have been experimentally determined. The enzymatic efficiency has been
evaluated through temperature optimum, pH optimum, Km and other enzymatic properties along with spectroscopic parameters
like circular...
Tipo: Presentation
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6410/version/1
A new potential radiosensitizer: ammonium persulfate modified WCNTs
Provedor de dados: 17
Autores: Jian-She Yang; Bo-Zhang Yu; Wen-Xin Li.
Radiotherapy plays a very important role in cancer treatment. Radiosensitizers have been widely used to enhance the
radiosensitivity of cancer cells at given radiations. Here we fabricate multi-walled carbon nanotubes with ammonium persulfate,
and get very short samples with 30-50 nanometer length. Cell viability assay show that f-WCNTs induce cell death significantly.
We hypothesize that free radicals originated from hydroxyl and carbonyl groups on the surface of f-WCNTs lead cell damage.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1421/version/1
Multi-Cancer Computational Analysis Reveals Metastasis-Associated Variant of Desmoplastic
Reaction Involving INHBA and THBS2
Provedor de dados: 17
Autores: Hoon Kim; John Watkinson; Vinay Varadan; Dimitris Anastassiou.
Despite extensive research, the details of the metastasis-associated biological mechanisms are largely unknown. Here, we analyze
data from multiple cancers using a novel computational method identifying sets of genes whose coordinated overexpression
indicates the presence of a particular phenotype. We conclude that there is one shared “core”
metastasis-associated gene expression signature corresponding to a specific variant of desmoplastic reaction, present in a large
subset of samples that have exceeded a threshold of invasive transition specific to each cancer, indicating that the biological
mechanism is triggered at that point. For example this threshold is reached at stage IIIc in ovarian cancer and at stage II in
colorectal...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4503/version/1
The effects of anti-sense interleukin-5 gene transferred by recombinant adeno-associated virus in
allergic rats
Provedor de dados: 17
Autores: Daxiong Zeng; Yong Cao; Qingfeng Song; Chao Cao; Xiansheng Liu; Yongjian Xu; Weining Xiong.
The accumulation and infiltration of eosinophils in airways is one of the most important characteristics of asthma, and is mediated
partly by secretion of IL-5 from Th2 lymphocytes. It is well known that interleukin-5 (IL-5) played an important role in the
regulation of eosinophils. In this study, an anti-sense IL-5 gene transferred by recombinant adeno-associated virus
(rAAV-ASIL-5) was prepared to transfect allergic rats. It was found that the expression of IL-5 protein in plasma and BALF were
inhibited significantly. The rAAV-ASIL-5-mediated suppression of total cell counts in peripheral blood and BALF were also
observed. Moreover, rAAV-ASIL-5 remarkably reduced the eosinophil counts in peripheral blood and BALF, as well as the
expression of ECP protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2036/version/1
Magnetic Resonance imaging (MRI) in detection of _Bifidobacterium longum_ and _Clostridium
novyi-NT_ labeled with superparamagnetic iron oxide (SPIO) nanoparticle
Provedor de dados: 17
Autores: Jianhua Wang; Xuebin Zhang; Dayong Zhou; Qingguo Wang; Dan Li.
*Purpose:* To investigate the MR imaging of _Bifidobacterium longum_ and _Clostridium novyi-NT_ labeling with
superparamagnetic iron oxide (SPIO) nanoparticles.

*Materials and methods:* Tubes
containing _B. longum_-SPIO, Free-SPIO, _B. longum_ and PYG Medium were incubated under anaerobic condition in _in
vitro_ experiment. Transmission electron microscope and Prussian blue staining were used to demonstrate intra-bacteria
nanoparticles. R~2~^*^ mapping and R~2~ mapping were reconstructed after MR scanning. _B. longum_-SPIO and _C.
novyi_-NT-SPIO
were
injected
respectively
_in
vivo_
to
show
whether
it
might
be
traced
by
MR
imaging.

*Results:* Magnetosomes in bacteria were observed by...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2009
URL: http://precedings.nature.com/documents/3435/version/1
GO faster ChEBI with Reasonable Biochemistry
Provedor de dados: 17
Autores: Duncan Hull.
Chemical Entities of Biological Interest (ChEBI) is a database and ontology that represents biochemical knowledge about small
molecules. Recent changes to the ontology have created new opportunities for automated reasoning with description logic, that
have not previously been fully exploited in Chemistry. These changes open up the possibility of building an improved chemical
semantic web, by making more use of necessary and sufficient conditions, allowing reasoning about chemical structure,
highlighting ambiguous inconsistencies and improving alignment with the Gene Ontology (GO). This paper briefly discusses
some of the problems with reasoning over the current version of ChEBI, to tackle these issues, and their potential solutions.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2329/version/1
A Reaction Route Approach to Flux Balance Analysis
Provedor de dados: 17
Autores: Ilie Fishtik.
Background: Flux balance and network-based pathway analyses are theoretical tools aimed to find optimal steady state flux
distributions in a metabolic network subject to additional constraints on the rates of the reaction steps. Although these methods are
mathematically accurate, there are several physicochemical and computational aspects that are questionable and misleading. In
particular, it is well known that the flux balance analysis may result in multiple flux distributions for the same objective function.


Results: The flux balance and network-based pathway analyses are reformulated in
terms of reaction routes (RRs), a theoretical framework that has been developed by Horiuti over 50 years ago. Not only does the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/2788/version/1
Iron (FeSO4) Bioavailability in Obese Subjects Submitted to Bariatric Surgery
Provedor de dados: 17
Autores: Luciana Bueno; Juliana Pizzo; José Ernesto dos Santos; Julio Sérgio Marchini; José
Eduardo Dutra de Oliveira.
Objective: To determine iron bioavailability in obese subjects after the ingestion of a nutritional supplement containing multiple
nutrients. Methods: Fourteen persons were studied before and after bariatric surgery after the ingestion of a nutritional formulation
containing 25 mg iron, 25 g fiber and 800 mg calcium. Serum iron levels were measured basally and every hour after the ingestion
of 200 mL of the formulation, and serum ferritin, unsaturated iron binding capacity (UIBC), hemoglobin, albumin, total proteins,
total triglycerides, total cholesterol and fractions (LDL-cholesterol and HDL-cholesterol) sere determined at the same time
points.Results: The following ferremia values (median/minimum - maximum) were obtained before and after bariatric...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3787/version/1
Analysis of plant metabolites – the baseline-threshold principle
Provedor de dados: 17
Autores: Katja Neubauer; Jenny Bandomir; Anett Grunenberg; Andreas Müller; Udo Kragl; Kerstin Schmidt.
With the environmental risk assessment (ERA) for genetically modified plants (GMP) the identification of equivalence or
differences in the composition of ingredients of GMPs and non-GMPs has to be investigated. The strategy is based upon the idea
that traditionally cultivated crops have been used safely for many years, thus every divergence within the compounds of a GMP in
contrast
to
its
non-GMP
counterpart
could
refer
to
a
risk
and
has
to
be
further
analysed.

The BioOK company offers the opportunity to analyse very different
aspects from the ERA of GMPs in one hand. Beside toxicological analysis and the determination of environmental effects via soil
analysis, we can look into several plant metabolites to identify...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4439/version/1
Development of a Decision Support System (DSS) for an integrated test system towards the effective
and competitive risk assessment on transgenic plants
Provedor de dados: 17
Autores: Christine Höflich; Andreas Müller; Jörg Schmidtke; Kerstin Schmidt; Inge
Broer.
Transgenic plants need specific approaches to analyse their potential impact on environment and consumer. The procedures used
to date are often too extensive and time-consuming and associated with high costs. The BioOK network was established to
develop an effective and competitive risk assessment on transgenic plants based on interdisciplinary research. New and effective
methods for the risk assessment were established which focus on transgene-specific effects measured in in vitro systems in the
laboratory or greenhouse whenever possible. Nevertheless event-specific field experiments have to be carried out in addition to
implement
environmental
effects
e.g.
on
non-targets
or
the
variability
of
transgene
expression.

The...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4438/version/1
Artificial Antigen Presenting Cells With Preclustered anti-CD28/-CD3/-LFA-1 Monoclonal Antibodies
Are Highly Effective To Induce The Ex-Vivo Expansion Of Functional Human Antitumor T Cells
Provedor de dados: 17
Autores: Roberta Zappasodi; Massimo Di Nicola; Carmelo Carlo-Stella; Roberta Mortarini; Alessandra Molla; Claudia Vegetti;
Lorena Passoni; Salvatore Albani; Andrea Anichini; Alessandro M. Gianni.
Effective adoptive T cell therapy requires the _ex vivo_ generation of functional T lymphocytes with a long lifespan _in vivo_.
We evaluated _in vitro_ T cell expansion by artificial antigen presenting cells (aAPC) generated with activating (human
anti-CD3), co-stimulating (human anti-CD28) and adhesion (human anti-LFA-1) monoclonal antibodies pre-clustered in
microdomains (MDs) held by a liposome scaffold. The co-localization of T cell ligands in MDs and the targeting of an adhesion
protein, increasing the efficiency of immunological synapse formations, represent the novelties of our system. These aAPCs
allowed increased expansion of polyclonal CD4^+^ and CD8^+^ T cells and of tumor antigen-specific CD8^+^ T cells compared
to anti-CD28- and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2007
URL: http://precedings.nature.com/documents/1250/version/1
The History, Development and Impact of Computed Imaging in Neurological Diagnosis and
Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed
diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the
advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities,
forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama
of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/3
A novel strategy for the targeted analysis of protein and peptide metabolites
Provedor de dados: 17
Autores: Nicholas A. Williamson; Charles Reilly; Chor-Teck Tan; Sri-Harsha Ramarathinam; Alun Jones; Christie L. Hunter;
Francis R. Rooney; Anthony W. Purcell.
The detection and quantitation of exogenously administered biological macromolecules (e.g. vaccines, peptide and protein
therapeutics) and their metabolites is frequently complicated by the presence of a complex endogenous mixture of closely related
compounds. We describe a method that incorporates stable isotope labeling of the compound of interest allowing the selective
screening of the intact molecule and all metabolites using a modified precursor ion scan. This method involves monitoring the low
molecular weight fragment ions produced during MS/MS that distinguish isotopically labelled material from related endogenous
compounds. All isotopically labelled substances can be selected using this scanning technique for further analysis whilst other...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/3812/version/1
PMEM of carabid beetles in the agro-ecosystem: Prospects and problems
Provedor de dados: 17
Autores: Kai U. Priesnitz; Ullrich Benker.
The comparison of carabid communities sampled at three different field sites during 5 years of ecological biosafety research
provides useful information for the design of a post market environmental monitoring of this arthropod family. Each evaluated
field site demonstrated a unique composition of carabid species. A baseline for the carabid beetle communities would therefore be
necessary for each field site to detect any changes in its composition over time. Aside from the influence of the location, the
impacts of weather, the date of sampling and of course the chosen sampling method have to be considered. A standardized method
is a basic requirement for a replicable assessment. The number of traps per square meter, and the size and the design of the traps...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4445/version/1
SBML Level 3 Hierarchical Model Composition
Provedor de dados: 17
Autores: Lucian P. Smith; Michael Hucka.
A new proposal for incorporating modularity (or 'model composition') into SBML Level 3.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5133/version/1
Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular
lesions
Provedor de dados: 17
Autores: Hiroyuki Koyama; Hideo Kagaya; Makoto Oba; Yutaka Miura; Takehiko Ishii; Tsuyoshi Takato; Kazunori Kataoka;
Tetsuro Miyata.
Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of
appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK)
peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units
(PEG-PAsp(DET)). cRGD recognizes αvβ3 and αvβ5 integrins, which are
abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(DET) (cRGD-PEG-PAsp(DET)) formed polyplex
micelles through complexation with plasmid DNA (pDNA), and the cRGD-PEG-PAsp(DET) micelles achieved significantly more
efficient gene expression and cellular uptake as compared with...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5020/version/1
The Hepatocellular Hypoxia Criteria:2’Nitroimidazole Effect on Hepatocyte
Carbohydrate Metabolizing Enzymes
Provedor de dados: 17
Autores: Rakesh Sharma.
Aim:
to understand the 2’-nitroimidazole induced hypoxia and liver cell interaction, we proposed a
“Hapatocellular Hypoxia Criteria”. Hypothesis: The nitroimidazole induced metabolic energy loss and
oxygen depletion (hypoxia) in liver cell mitochondria causes the phagocytosis.
Based on it, ten control subjects with
2’-nitroimidazole therapy were studied for their carbohydrate metabolizing enzymes in serum and hepatocellular
enzymes in liver biopsy tissues. Materials and Methods: Proven ten control subjects were studied for hypoxia by enzyme assays.
The 2’nitroimidazole treated paired ten subjects were studied for hypoxia using enzyme assays and hepatocellular
cytomorphology by electron microscopy....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2738/version/1
Is there a Weed Shift in Roundup Ready Maize?
Provedor de dados: 17
Autores: Arnd Verschwele.
The cultivation of transgenic glyphosate-resistant crop varieties can simplify weed control in crops such as maize and others. On
the other hand, the repeated use of broadly effective herbicides like glyphosate, especially in the same crop, can have a negative
effect on biodiversity. Additionally there is a risk for a spread of more tolerant or even resistant weed
species.

It was the aim of a 6-years field study (2003-2008) to investigate weed
effects of use of glyphosate in a continuous transgenic herbicide-resistant maize rotation at three sites in Germany. These sites
differed significantly in terms of soil, climate and initial weed spectrum. The studies focussed on the comparison between local
herbicide standards and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4527/version/1
On the status of the Michaelis-Menten equation and its implications for enzymology
Provedor de dados: 17
Autores: Sosale Chandrasekhar.
The Michaelis-Menten equation (MME) is considered to be the fundamental equation describing the rates of enzyme-catalysed
reactions, and thus the 'physicochemical key' to understanding all life processes. It is the basis of the
current view of enzymes as generally proteinaceous macromolecules that bind the substrate reversibly at the active site, and
convert it to the product in a relatively slow overall sequence of bonding changes ('turnover'). The
manifested 'saturation kinetics', by which the rate of the enzymic reaction (essentially) increases linearly
with the substrate concentration ([S]) at low [S] but reaches a plateau at high [S], is apparently modelled by the MME. However,
it is argued herein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Evolutionary Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1637/version/1
Why not "do simple things in a simple way": Use of the Pap test as the first
step in screening genetic stability for human cultured stem cell therapy?
Provedor de dados: 17
Autores: Katherine Athayde Teixeira de Carvalho; Ana Carolina Irioda; Larissa Zocche; Carolina Maria Costa de Oliveira Souza;
Reginaldo J. Ferreira; Eduardo Aliprandini; Ricardo C Cunha; Julio C Francisco; Luiz Cesar Guarita-Souza; Mariester Malvezzi;
Miriam Beltrame; Lismary Mesquita; Diogo Kuczera; Rafael Vargas.
The aim of this study was to analyze adipose tissue-derived mesenchymal stem cells (AT-MSCs) using the Pap test as a first
screening step to evaluate genetic stability. Human adipose tissue from six healthy female donors was obtained from elective
liposuction procedures. The cells were isolated, cultivated at P2/P3, characterized by flow cytometric analysis, and differentiation
induced. The AT-MSCs were stained by Papanicolaou staining and analyzed according to the Bethesda classification, and
viability-apoptosis relationships were evaluated. The results of the Pap test for Sample I indicated high-grade alterations
consistent with genetic instability; for Samples II-V, atypical cells of undetermined significance; and for Sample VI, normal cells.
These...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5676/version/1
HMGB1 release by C5a anaphylatoxin is an effective target for sepsis treatment
Provedor de dados: 17
Autores: Hidechika Okada; Noriko Okada; Masaki Imai; Alan Okada; Fumiko Ono.
Antibodies to C5a have proven to be effective in treating experimental septic primate models. A 17 amino acid peptide
(ASGAPAPGPAGPLRPMF) named PepA binds to C5a and prevents complement-mediated lethal shock in rats. AcPepA
harboring an acetyl group at the N-terminal alanine showed increased inhibitory activity against C5a. Cynomolgus monkeys
destined to expire from a lethal dose of bacterial endotoxin (4mg/kg) were rescued by intravenous administration of AcPepA.
AcPepA could have interfered with the ability of C5a to stimulate C5L2 which is responsible for HMGB1 release and stimulation
of TLR4 as an endogeneous ligand with LPS behavior. The suppression of HMGB1 release by AcPepA administration to
LPS-shock monkeys is likely responsible for rescuing the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/5727/version/1
Sequence-specific Solution Structures of the Four Isosequential Pairs of Single-stranded DNAs and
RNAs
Provedor de dados: 17
Autores: Subhrangsu Chatterjee; Wimal Pathmasiri; Jyoti Chattopadhyaya.
The role of the sequence-context in the self-organization of four single-stranded (ss) isosequential pairs of DNAs (1
– 4) and RNAs (5 – 8), [d/r-(5'C^1^A^2^X^3^G^4^Y^5^A^6^C^7^): X^3^ = A or C,
Y^5^ = A or C; sequence variations: 2^2^ = 4], has been elucidated by NMR-constrained Molecular Dynamics (MD) simulations
(2 ns). Following sequence-specific observations have been made from the solution NMR and the NMR constrained MD
simulation study: (i) Analysis of the NOESY footprints, mainly (H8/H6)~n~ to (H1' and H3')~n-1~
contacts, of ssDNAs (1 - 4) and ssRNAs (5 – 8) in the aqueous medium have shown that all ssDNAs (1 - 4) and
ssRNAs (5 - 8) adopt right handed stacked helical structures in the NMR...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1685/version/1
Phytochemical Approach and Bioanalytical Strategy to Develop Chaperone-Based Medications
Provedor de dados: 17
Autores: Bernd B. K. Kastenholz.
Currently, no pharmaceuticals for the etiological treatment of degenerative protein-misfolding diseases (e.g., ALS,
Alzheimer’s or prion diseases) are commercially available. Therefore, in this technical note theoretical
considerations and practical approaches concerning the development of chaperone-based medications from medicinal plants (e.g.,
Ginkgo biloba) are reviewed and discussed in detail. Phytochaperones and other agents isolated from medicinal plants are
proposed to serve as the general basis of drug development in protein-misfolding diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Pharmacology.
Ano: 2007
URL: http://precedings.nature.com/documents/1431/version/1
Analysis of plant metabolites – the baseline-threshold principle
Provedor de dados: 17
Autores: Katja Neubauer; Jenny Bandomir; Anett Grunenberg; Andreas Müller; Udo Kragl; Kerstin Schmidt.
With the environmental risk assessment (ERA) for genetically modified plants (GMP) the identification of equivalence or
differences in the composition of ingredients of GMPs and non-GMPs has to be investigated. The strategy is based upon the idea
that traditionally cultivated crops have been used safely for many years, thus every divergence within the compounds of a GMP in
contrast
to
its
non-GMP
counterpart
could
refer
to
a
risk
and
has
to
be
further
analysed.

The BioOK company offers the opportunity to analyse very different
aspects from the ERA of GMPs in one hand. Beside toxicological analysis and the determination of environmental effects via soil
analysis, we can look into several plant metabolites to identify...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4439/version/2
Biodegradable Luminescent Silicon Quantum Dots for Two Photon Imaging Applications
Provedor de dados: 17
Autores: Chen-An Tien; Folarin Erogbogbo; Ching-Wen Chang; Priscilla Adjei-Baffour; Wing-Chueng Law; Mark T.
Swihart.
Cadmium- and lead-based quantum dots are normally coated for biological applications, because their degradation may result in
the release of toxic heavy metal ions. Here, we synthesize silicon quantum dots that are expected to biodegrade to non-toxic
products. A chitosan coating is used to render the silicon quantum dots stable in storage conditions and biodegradable at
physiological conditions. The applications of these particles are demonstrated in cellular imaging with single and two-photon
excitation. These results open the door for a new generation of silicon quantum dots that may have a wide variety of applications
derived from the flexibility of chitosan.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2012
URL: http://precedings.nature.com/documents/7012/version/1
Standardising bioresource acknowledgement in papers
Provedor de dados: 17
Autores: Dr Robert Hewitt.
This presentation describes the speakers experience of gathering performance indicator data, for biobank assessment. Problems
encountered in collecting data on the number of publications resulting from supported studies are described. These include
difficulty in obtaining a complete record of all the resulting publications and also frequent misnaming of the biobank in the
acknowledgment section of papers. The presentation also includes a discussion of approaches to providing credit to all the
contributors in the biobanking effort.



Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5607/version/1
Iridium complex, a phosphorescent light-emitting diode material, serves as a novel chemical probe for
imaging hypoxic tumor tissues
Provedor de dados: 17
Autores: Shaojuan Zhang; Masahiro Hosaka; Toshitada Yoshihara; Yasuhiko Iida; Keigo Endo; Seiji Tobita; Toshiyuki
Takeuchi.
Iridium complex, a promising organic light-emitting diode for next generation television displays, emits phosphorescence.
Phosphorescence is quenched by oxygen. We used this oxygen-quenching feature for imaging tumor hypoxia. Red light-emitting
iridium complex Ir(btp)~2~(acac) (BTP) presented hypoxia-dependent light emission in culture cell lines, whose intensity was in
parallel with HIF-1[alpha] expression. BTP was further applied to imaging five tumors (four from human origin and one from
mouse origin) transplanted in athymic mice. All tumors presented a bright BTP-emitting image even 5 min after the injection. The
BTP-dependent tumor image peaked at 1 to 2 h after the injection, and was then cleared from tumors within 24 h. The minimal
BTP image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1443/version/1
CFP and YFP photostabilities are differentially affected by common mounting fluids
Provedor de dados: 17
Autores: Johannes A. Schmid.
The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP,
respectively) is very common in all different fields of life sciences, e.g. for marking and tracing of specific proteins or cells or to
determine protein interactions. In the later case, the quantum physical phenomenon of fluorescence resonance energy transfer
(FRET) is visualized by specific microscopy techniques. When we applied a commonly used FRET microscopy technique - the
increase in CFP-fluorescence after bleaching of YFP, we noticed that it worked well for live cells, but that most of the
FRET-signal was lost in fixed cells mounted in commercial microscopy mounting fluids. Subsequently, we could show that CFP
bleached much faster...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4517/version/1
A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with
Unprecedented Stereoselectivity
Provedor de dados: 17
Autores: Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for
the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties
and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate
ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a
unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia
coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/1697/version/2
The History, Development and Impact of Computed Imaging in Neurological Diagnosis and
Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed
diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the
advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities,
forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama
of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/1
Biotechnology research in Venezuela: Five case studies
Provedor de dados: 69
Autores: Morales,Rosa.
The organization of biotechnology differs across countries. The structure of the industry, the academia, and the links between
these two sectors vary according to which country is studied. Biotechnology is organized in Venezuela mainly around the research
done by special research and development units at national universities. The paper describes some of these units in order to shed
light about their performance. Two economic approaches are used to explain the academic production of these biotech research
organizations: the technological and organizational approaches. A case study methodology is used in this research. The results can
be summarized as: The percentage of researchers who are doctors is less than seventy percent in each research and...
Tipo: Journal article
Palavras-chave: Human capital; Latin American biotechnology; Research and development units; Organization of biotechnology.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500001
Date and their processing byproducts as substrates for bioactive compounds production
Provedor de dados: 52
Autores: Tang,Zhen-Xing; Shi,Lu-E; Aleid,Salah Mohammed.
Date is the most popular fruit in middle-east countries. However, the date production process is accompanied by a substantial
increase of loss during picking, storage, commercialization and conditioning process. Date and their byproducts have many
essential elements for the growth of microorganisms. Thus, they can be converted into value-added compounds through
biotechnology. In this paper, date and their processing byproducts used as substrates for producing value-added products such as
organic acids, exopolysaccharide, antibiotics, date flavored probiotic fermented dairy, bakery yeast, etc, were reviewed.
Tipo: Journal article
Palavras-chave: Date; Byproduct; Biotechnology; Fermentation; Review.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500706
Riverina high school students’ views of biotechnology
Provedor de dados: 69
Autores: Cavanagh,Heather; Hood,Jennie; Wilkinson,Jenny.
A survey investigating the knowledge and perception of biotechnology by high school students living in the rural Riverina region
of New South Wales, Australia, was undertaken. Data relating to the student’ preferred and trusted information sources was also
gathered. This study has demonstrated that at least two-thirds of students had a good knowledge of medical biotechnology issues,
however, a significant proportion of the students did have concerns about the use and/or safety of biotechnology. Nearly 90% of
the respondents would like further information on biotechnology. In agreement with other surveys, the students reported an
apparent lack of trust in articles from the lay press (newspapers, television, radio) and yet still report that these media as...
Tipo: Journal article
Palavras-chave: Biotechnology; Education; Science; Secondary school.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200001
Prediction of miRNAs in Bombyx mori through Computational Approaches
Provedor de dados: 28
Autores: Mishra, A K; Lobiyal, D K.
In this paper, we have used rule-based approach to predict mature miRNA from pre-miRNAs of Bombyx moori (Silk worm). For
rule based approach, it is important to have a set of rules. Unfortunately, to the best of our knowledge, miRNA related literature
does not provide well-defined set of rules for miRNA prediction. Therefore, the challenge in this research was to derive a set of
rules from known data set of related species. We have made an attempt to derive a set of rules from the known miRNA and
pre-miRNA data sets of Drosophila melanogaster and Apis mellifera. The derivation of rules is based on the dominating features
identified using PCA and infogain method applied by authors in their previous work. Based on these rule set a PERL script is
written to...
Tipo: Article
Palavras-chave: Agricultural Sciences and Technology; Biotechnology; Bioinformatics.
Ano: 2010
URL: http://eprints.iari.res.in/54/1/149-G237.pdf
STMS-based DNA fingerprints of the new plant type wheat lines
Provedor de dados: 28
Autores: Mohapatra, T; Krishanpal, Krishanpal; Singh, S S; Swain, S C; Sharma, R K; Singh, N K.
New plant type wheat lines have been developed at the Indian Agricultural Research Institute, New Delhi, by combining some of
the negatively correlated yield con- tributing traits such as more grains per panicle, lar- ger grain size and optimum tillers per plant
through inter-varietal hybridization followed by a modified- bulk pedigree method of selection. Some of these lines have been
found to give 15–22% higher yield in ex- perimental plots than the most popular national check varieties being cultivated in the
north-western part of India and thus constitute a valuable source of genetic variation for breaking yield barriers in wheat. With the
objective of precise differentiation and identifica- tion of new plant type wheat lines, 33 sequence tagged...
Tipo: Article
Palavras-chave: Genetics and Plant Breeding; Biotechnology; Genetics.
Ano: 2003
URL: http://eprints.iari.res.in/135/1/1125.pdf
A three-dimensional stochastic spatio-temporal model of cell spreading
Provedor de dados: 17
Autores: Yuguang P. Xiong; Padmini Rangamani; Benjamin Dubin-Thaler; Michael Sheetz; Ravi Iyengar.
Cell motility is important for many physiological processes and the underlying
biochemical reactions
motility have been well characterized. Mathematical models, using
the biochemical reactions and focused
on different types of spreading behavior have been
constructed and analyzed. In this study, we build on
these previous models to develop a
three-dimensional stochastic model of isotropic spreading of mammalian
fibroblasts. The model is composed of three actin remodeling reactions that occur stochastically in space and time and are
regulated by membrane resistance forces. Numerical simulations indicate that the model qualitatively captures the experimentally
observed isotropic cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/62/version/2
Identification of a highly polymorphic tetranucleotide repeat locus (DXpS) at Xp and development of
a DXpS/HUMARA biplex methylation-based PCR assay that enhances detection of X-chromosome
inactivation
Provedor de dados: 17
Autores: Filipe B. Machado; Ester S. Ramos; Pamela C. Radic; Carlos D. de Brasi; Enrique Medina-Acosta.
The methylation-based PCR assay at the polymorphic (CAG)n repeat in exon 1 of the human androgen receptor _AR_ gene
(HUMARA) is a standard method for determination of the methylation state of alleles in active X (Xa) and inactive X (Xi)
chromosomes. HUMARA assay is endowed with heterozygosity rates ~85% worldwide. This means that in a proportion of
females it is uninformative. The HUMARA genotype is not neutral, being linked to Kennedy´s disease. Moreover,
allele designation and quantification from trinucleotide repeats demand normalizing for minor (stutter) products differing from the
original template by multiples of the repeat unit. Here, we report on the identification of a highly polymorphic tetranucleotide
repeat (named DXpS) mapping to...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics.
Ano: 2011
URL: http://precedings.nature.com/documents/6633/version/1
Analysis of acceptance of bacillus to various heavy metals and antibiotics
Provedor de dados: 17
Autores: Yamini Singh Sisodia; Vivek Bajpai; Deeksha Gaur; Pankaj Kumar Jain.
The present study was carried out to systematically examine the pathogenic microorganisms in sewage samples isolated from the
Shekhawati region of Rajasthan. In this survey, 26 bacterial strains were isolated and identified using biochemical and molecular
methods. Molecular identification of isolates was done by DNA isolation, PCR amplification using 16S rDNA primer and partial
sequencing of purified product after sequencing, out of 26, 10 sequences were submitted to NCBI. Out of these 10 isolates
Bacillus pumilis, Bacillus subtilis and Bacillus licheniformis were taken for further studies. There is growing awareness of the
need for development of new antimicrobial agents for the treatment of human, animal and plant diseases. Keeping this fact in
mind, we...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7073/version/1
pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic
Resistance Markers
Provedor de dados: 17
Autores: Corinne Marie; Magali Richard; Gaelle Vandermeulen; Mickael Quiviger; Veronique Preat; Daniel Scherman.
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of
dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Resistance genes, called pFARs. The strategy is based on the suppression of a chromosomal nonsense mutation by a
plasmid-borne function. The amber mutation was introduced into the Escherichia coli thyA gene that encodes a thymidylate
synthase required for dTMP synthesis, resulting in thymidine auxotrophy. In parallel, a small plasmid vector that carries an amber
suppressor t-RNA gene was entirely synthesised. The introduction of pFAR plasmids into an optimised thyA mutant restored
normal growth to the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics.
Ano: 2008
URL: http://precedings.nature.com/documents/2395/version/1
Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE
Provedor de dados: 17
Autores: Bernd Kastenholz; David E. Garfin.
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native
continuous polyacrylamide gel electrophoresis (QPNC-PAGE)
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4617/version/1
A Targeted Therapeutic Rescues Botulinum Toxin-A Poisoned Neurons
Provedor de dados: 17
Autores: Prabhati Ray; Bal Ram Singh; Radharaman Ray; Peng Zhang; Dan Li; James Keller; Michael Adler.
Botulinum neurotoxin (BoNT), a Category A biothreat agent, is the most potent poison known to mankind. Currently no antidote
is available to rescue poisoned synapses. BoNT acts specifically by blocking neurotransmission primarily at peripheral
nerve-muscle junctions causing severe flaccid muscle paralysis, which is fatal if proper medical care is not provided. The
neurotoxin acts by specifically entering the presynaptic nerve endings where it interferes with the biochemical machinery involved
in the process of neurotransmitter release, i.e., neuroexocytosis. Most serotypes of BoNT are known to remain active for weeks to
months after entering the nerves, but BoNT/A is the most potent and long lasting in causing muscle paralysis. An effective
medical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2149/version/1
Herbal Drugs in Mirror of Alzheimer’s Disease
Provedor de dados: 17
Autores: Bernd Kastenholz; Kerstin Nagel; David E. Garfin; Jürgen Horst.
Commercially available Ginkgo biloba leaf extracts may lack specificity as to the binding of copper and zinc ions in human cells.
This might be the reason why Ginkgo extracts have no predictable and clinically significant benefit for people with dementia or
cognitive impairment. A novel generation of herbal drugs is proposed as antioxidant and metal chelator in the treatment of
Alzheimer's disease.
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Pharmacology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3334/version/1
Proof of principle for shotgun DNA mapping by unzipping
Provedor de dados: 17
Autores: Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch.
Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on
key cellular processes. Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates
this importance, but key information is elusive in these ensemble methods. We’re pursuing a new single-molecule
chromatin mapping method based on unzipping native chromatin molecules with optical tweezers. The first step we are taking
towards this ability is shotgun DNA mapping (SDM). This is the ability to identify the genomic location of a random DNA
fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome. We show
that ~32 separate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics.
Ano: 2010
URL: http://precedings.nature.com/documents/2808/version/2
The Entropic Landscape of proteins revealing protein folding mechanism
Provedor de dados: 17
Autores: Kentaro Onizuka.
It has long since been a mystery why most proteins fold within a flash of time into particular structures out of astronomically large
numbers of possible conformations. Even more confusing is that protein folding in vivo is played out in rich solution containing
various organic and non-organic, big and small molecules and ions which would potentially bind the protein molecules and
prevent them folding. A possible answer to these mysteries might be, "Nature have favoured such proteins that quickly
fold in rich solution through natural selection". Then what mechanism of folding has been favoured? Here I show how
to decipher protein sequences to reveal the folding mechanism. The entropic landscape of a protein sequence tells which region
of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2719/version/1
Cancer invasion associated gene expression signature is present in differentially expressed genes in the
reprogramming of fibroblasts into stem cells
Provedor de dados: 17
Autores: Wei-yi Cheng; Hoon Kim; Jessica Kandel; Dimitris Anastassiou.
Tumors become invasive by penetrating adjacent connective tissue, but the underlying biological mechanisms remain obscure. We
recently identified a precise gene expression signature of fibroblastic origin associated with cancer invasion, the first step of the
metastatic cascade. The signature contains many coordinately overexpressed genes, prominent among which are COL11A1,
THBS2 and INHBA. Here we show that there is a striking similarity between the set of expressed genes in this
metastasis-associated fibroblastic (MAF) signature and the set of genes that are downregulated when fibroblasts are
reprogrammed to induced pluripotent stem cells (iPSCs). Because it is known that fibroblast reprogramming involves a
mesenchymal epithelial transition (MET), the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5924/version/1
Evaluation of effective parameters on fabrication of BSA nanoparticles
Provedor de dados: 17
Autores: Mostafa Rahimnejad; Ghasem Najafpour; Mohsen Jahanshahi.
Coacervation technique was implemented in fabrication of BSA nanoparticle for the purpose of drug delivery system.
Nanoparticle was purified with 48,800 g centrifuge, and then followed by dialysis, micro and ultra filtration. The fabricated
nanoparticles were subjected to physical, morphological and biochemical characterization. The analyzed samples by SEM and
AFM proved the semi-spherically shape of the particles whilst the size distribution was measured by photon correlation
spectroscopy. Fourier transforms infra-red spectroscopy (FTIR) spectrum indicated that the fabricated nanoparticles were pure.
The impact of various parameters upon the particle size was extensively investigated. The concentration of glutaraldehyde at fixed
temperature had absolutely...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2966/version/1
Lego DNA assembling, a simple in vitro method for constructing DNA molecule
Provedor de dados: 17
Autores: Mo Xian; Xinglin Jiang; Jianming Yang; Haibo Zhang; Huaiwei Liu; Yanning Zheng; Cong Wang; Sizheng Su.
Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in
molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can
radically simplify some of the same task. The lego DNA assembling, based on strand annealing, allows in vitro assembly of multi
DNA fragments in one step with precise junctions and excludes the need for any enzyme. As a proof of concept, we rapidly
constructed plasmids from 4, 6 and 8 fragments with very high efficiencies (100%). And we found this method a powerful tool for
synthetic biology, constructing a partial isoprene biosynthesis pathway (consisting of four genes) in 2 days. We also assembled a
customized...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics.
Ano: 2010
URL: http://precedings.nature.com/documents/5147/version/1
Development of Novel Calibrations for FT-NIR Analysis of Protein, Oil, Carbohydrates and
Isoflavones in Foods
Provedor de dados: 17
Autores: I Baianu; Jun Guo.
The development of calibration methodology for novel FT-NIRS analysis of soybean-based foods is presented together with
high-precision NIRS spectra and composition measurements in terms of proteins, oil and carbohydrates in soybean-based
foods/soy foods.

Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6317/version/1
Spiral Tissue Microarrays as Next Evolutionary Step in the High-density Tissue Microarray
Technology
Provedor de dados: 17
Autores: Junya Fukuoka; Matthias Hofer; Takashi Hori; Tomonori Tanaka; Sayaka Tominaga; Ryo Ohsawa; Takeshi Uemura;
Lucian Chirieac.
Tissue microarray (TMA) is a well-established technique that connects basic research with clinical applications that allow the
validation of many pathobiologic events from gene expression dysregulation to genomic aberrations. However, conventional
TMAs have several limitations such as limited representation of tissue heterogeneity, destruction of donor tissue blocks due to
coring and usage of particular specimens that have limited evaluable material (tissue from thin specimens or needle biopsies). We
have developed a novel method, which we termed "Spiral TMA" that generates TMAs that allow for
improved representation of the donor tissue while keeping the architectural details of the donor block intact. This technology is
ideal for...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4831/version/1
Serine biosynthesis with one carbon catabolism represents a novel pathway for ATP generation in
cells using alternative glycolysis with zero net ATP production
Provedor de dados: 17
Autores: Alexei Vazquez; Elke K. Markert; Zoltan N. Oltvai.
Recent experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP
production, implying that upregulation of glycolysis in these cells may not be related to the generation of ATP. Here we use a
genome-scale model of human cell metabolism to investigate the potential metabolic alterations in cells using net zero ATP
glycolysis. We uncover a novel pathway for ATP generation that involves reactions from the serine biosynthesis and one-carbon
metabolism pathways. This pathway has a predicted two-fold higher flux rate in cells using net zero ATP glycolysis than those
using standard glycolysis and generates twice as much ATP with significantly lower rate of lactate- but higher rate of alanine
secretion. Thus,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6123/version/2
Empirical predictions of an intrinsically disordered protein theory approach to glycan/lectin reaction
kinetics
Provedor de dados: 17
Autores: Rodrick Wallace.
Newly-developed methods from the theory of intrinsically disordered proteins can be applied to the flexible glycan structures that
coat cellular surfaces and provide rich channels for biological information transmission. Extension of a mechanistic
'arm-in-sleeve' model via a nonrigid molecule symmetry analysis leads to expectation of empirical
observation of punctuated 'spectral' classifications in glycan/lectin interaction, parameterized by an
appropriate index of glycan frond length or other index of topological complexity, possibly requiring groupoid classifications
analogous to quasicrystals.
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6752/version/1
Molecular Static and Dynamic Analyses reveal Flaw in Murine Model used by US FDA to Detect
Drug Carcinogenicity
Provedor de dados: 17
Autores: Trevor Marshall.
The US FDA currently accepts carcinogenicity studies of pharmaceutical drugs based on murine models. In addition to 6 month
studies with p53(+/-) and ras.H2 transgenic mice, lifetime studies (typically 2 years) in WT mice or rats are also considered as
evidence that a drug lacks carcinogenic activity. This model is not always exhaustive. For example, during the acceptance testing
of the ARB Olmesartan[1], possible carcinogenicity observed in hamsters was not able to be duplicated in rats, or in transgenic
mice. We have previously used the static molecular modeling of AutoDock to demonstrate that Olmesartan has agonostic activity
in the PDB:1DB1 model of the human VDR Nuclear Receptor[2], while it has antagonistic activity in the PDB:1RK3 model of the
rat...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Immunology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/52/version/1
PROM Khad an efficient source of P to replace the costly chemical Phosphatic Fertilizer
Provedor de dados: 17
Autores: DMR Sekhar; MK Katewa; MS Shaktawat.
The subsidy on phosphate fertilizers rose to an unacceptable level while alternate cost effective technologies are available.
Fertilizer industry needs to develop innovative products using the recent advances in the agricultural sciences. Manure producing
industries to be supplied rock phosphate mineral (at subsidized costs) as being supplied to chemical fertilizers industries suitable
to produce PROM Khad (Phosphate Rich Organic Manure).
Tipo: Manuscript
Palavras-chave: Biotechnology; Earth & Environment.
Ano: 2012
URL: http://precedings.nature.com/documents/6848/version/1
Genes2Networks: Connecting Lists of Proteins by Using Background Literature-based Mammalian
Networks
Provedor de dados: 17
Autores: Jeremy I. Posner; Seth Berger; Avi M. Ma'ayan.
In recent years, in-silico literature-based mammalian protein-protein interaction network datasets have been developed. These
datasets contain binary interactions extracted manually from legacy experimental biomedical research literature. Placing lists of
genes or proteins identified as significantly changing in multivariate experiments, in the context of background knowledge about
binary interactions, can be used to place these genes or proteins in the context of pathways and protein
complexes.
Genes2Networks is a software system that integrates the content of ten mammalian
literature-based interaction network datasets. Filtering to prune low-confidence interactions was implemented. Genes2Networks is
delivered as a web-based service using...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/35/version/2
Proof of principle for shotgun DNA mapping by unzipping
Provedor de dados: 17
Autores: Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch.
Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on
key cellular processes. Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates
this importance, but key information is elusive in these ensemble methods. We’re pursuing a new single-molecule
chromatin mapping method based on unzipping native chromatin molecules with optical tweezers. The first step we are taking
towards this ability is shotgun DNA mapping (SDM). This is the ability to identify the genomic location of a random DNA
fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome. We show
that ~32 separate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics.
Ano: 2009
URL: http://precedings.nature.com/documents/2808/version/1
Serine biosynthesis with one carbon catabolism represents a novel pathway for ATP generation in
cells using alternative glycolysis with zero net ATP production
Provedor de dados: 17
Autores: Alexei Vazquez; Zoltan N. Oltvai.
Recent experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP
production, implying that upregulation of glycolysis in these cells may not be related to the generation of ATP. Here we use a
genome-scale model of human cell metabolism to investigate the potential metabolic alterations in cells using net zero ATP
glycolysis. We uncover a novel pathway for ATP generation that involves reactions from the serine biosynthesis and one-carbon
metabolism pathways. This pathway has a predicted two-fold higher flux rate in cells using net zero ATP glycolysis than those
using standard glycolysis and generates twice as much ATP with significantly lower rate of lactate- but higher rate of alanine
secretion. Thus,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6123/version/1
Genes2Networks: Connecting Lists of Proteins by Using Background Literature-based Mammalian
Networks
Provedor de dados: 17
Autores: Avi Ma'ayan.
In recent years, in-silico literature-based mammalian protein-protein interaction network datasets have been developed. These
datasets contain binary interactions extracted manually from legacy experimental biomedical research literature. Placing lists of
genes or proteins identified as significantly changing in multivariate experiments, in the context of background knowledge about
binary interactions, can be used to place these genes or proteins in the context of pathways and protein
complexes.
Genes2Networks is a software system that integrates the content of ten mammalian
literature-based interaction network datasets. Filtering to prune low-confidence interactions was implemented. Genes2Networks is
delivered as a web-based service using...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/35/version/1
Molecular crowding defines a common origin for the Warburg effect in proliferating cells and the
lactate threshold in muscle physiology
Provedor de dados: 17
Autores: Alexei Vazquez.
Aerobic glycolysis is a seemingly wasteful mode of ATP production that is seen both in rapidly proliferating mammalian cells and
highly active contracting muscles, but whether there is a common origin for its presence in these widely different systems is
unknown. To study this issue, here we develop a model of human central metabolism that incorporates a solvent capacity
constraint of metabolic enzymes and mitochondria, accounting for their occupied volume densities, while assuming glucose
and/or fatty acid utilization. The model demonstrates that activation of aerobic glycolysis is favored above a threshold metabolic
rate in both rapidly proliferating cells and heavily contracting muscles, because it provides higher ATP yield per volume density
than...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5784/version/1
A Three dimesional stochastic spatio-temporal model of cell spreading
Provedor de dados: 17
Autores: Yuguang P. Xiong; Padmini Rangamani; Benjamin Dubin-Thaler; Michael Sheetz; Ravi Iyengar.
Cell motility is important for many physiological processes and the underlying
biochemical reactions
motility have been well-characterized. Mathematical models, using
the biochemical reactions and focused
on different types of spreading behavior have been
constructed and analyzed. In this study, we build on
these previous models to develop a
three-dimensional stochastic model of isotropic spreading of mammalian
fibroblasts. The model is composed of three actin remodeling reactions that occur stochastically in space and time and are
regulated by membrane resistance forces. Numerical simulations indicate that the model qualitatively captures the experimentally
observed isotropic cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/62/version/1
Generalized Theory of Life
Provedor de dados: 17
Autores: Vyacheslav L. Kalmykov.
The paper is mathematically, physically and biologically inspired - it is a deductive attempt of the biologist to find the most
appropriate language for modelling of life. Applications of mathematical structures - groupoids, groups, categories, functors and
monads, which are the most effective for the description of the complex objects, are discussed. Some new conceptions and
interpretations were introduced: unified classification of complex systems; the generalized conception about stationary states of
any autonomous agent ("Statology"); the total quantitative criterions of evolutionary direction; the
generalized group of symmetry. The definitions of information, entropy, organization, behavior, evolution, creation, life, culture,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7108/version/1
Visualization of the Phosphoproteomic Data from AfCS with the Google Motion Chart Gadget
Provedor de dados: 17
Autores: Huilei Xu; Avi Ma'ayan.
Results from multivariate molecular biological experiments become increasingly complex. Hence, the challenge of projecting
high-dimensional data onto few dimensions for effective data visualization is becoming increasingly important in Systems
Biology. Effective data visualization can summarize the activity of many variables over time as well as display relationships
between variables. Dynamic interactive visualization tools can provide scientists with ways of visually identifying relationship
and patterns, and improve communication of results on the web and in presentations. For this, interactive systems with animation
have great potential since they add dimensions to static images limited to two dimensions. Interactivity and animation is
particularly...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3074/version/1
Integration of the scientific literature into the Semantic Web: Facts from biomedical data resources
Provedor de dados: 17
Autores: Dietrich Rebholz; Samuel Croset; Christoph Grabmueller.
Scientific literature is the primary resource for relevant and innovative information. The integration of the literature with other
data resources in the biomedical research community generates overhead that can be avoided through the used of Semantic Web
Technology generating openly accessible data. Projects such as SESL and CALBC have producted significant amount of data that
that are ready for exploitation.
The tutorial will teach different approaches on how to integrate the scientific
literature with the content from biomedical databases, and will discuss the inferences that can be achieved. Furthermore, the
tutorial will point to the resources that are ready for use and enable integration of the literature at your discretion. A good...
Tipo: Presentation
Palavras-chave: Biotechnology; Immunology; Bioinformatics; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7011/version/1
Potential and environmental concerns of ethanol production from sugarcane molasses in Pakistan
Provedor de dados: 17
Autores: Tahir Rashid; Zafar Altaf.
Energy security and climate change imperatives require large-scale substitution of petroleum-based fuels as well as improved
vehicle efficiency4. Biofuels have become one of the fastest growing markets in the world - at 15% growth a year. In fact, until
recently, Pakistan was the second largest exporter of sugarcane ethanol to the European Union - a preferential status we have since
lost because of WTO obligations and dumping complaints. Several distilleries have planned to close down in light of this fact.
Instead of curbing production of fuel ethanol, however, we should redirect it to the domestic market. Pakistan produces around 2
million tons molasses annually and over 90% is exported earning only $47 million. This quantity of molasses will produce...
Tipo: Manuscript
Palavras-chave: Biotechnology; Earth & Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/1499/version/1
BioBrick Promoter Measurement Kit Instruction Sheets
Provedor de dados: 17
Autores: Jason Kelly.
Instructions for inserting a test promoter into the measurement kit plasmid for characterization as well as instructions for
measuring the activity of the test promoter in Standard Promoter Units (SPUs). These instructions were distributed to the teams
participating in the International Genetically Engineered Machines competition (iGEM).
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2028/version/1
Blueberry production in Chile: current status and future developments
Provedor de dados: 94
Autores: Retamales,Jorge B.; Palma,María J.; Morales,Yohanna A.; Lobos,Gustavo A.; Moggia,Claudia E.; Mena,Carlos A.
Chile has become a major actor in the blueberry industry as the most important supplier of off-season fresh fruit for the northern
hemisphere. Blueberry exports passed from US$ 30 million (around 4,000 tons) in 2000 to US$ 380 million (94,000 tons) in 2011.
The characteristics of the major blueberry growing regions (North, Central, South-central and South) are presented in terms of
acreage, varieties, management practices, extension of the harvest season, and soil and climatic conditions. Most fruit is from
highbush varieties, picked by hand and exported fresh by boat to United States. Largest proportion of fruit is exported from mid
December to late January, which coincides with lowest prices. The south-central region (latitudes 34º50' to 38º15' S) was in...
Tipo: Journal article
Palavras-chave: Biotechnology; Growth regulator and plant science.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000100008
Management of the appropriate agricultural biotechnology for small producers: Bolivia case study
Provedor de dados: 69
Autores: Avila,Teresa; Izquierdo,Juan.
The Bolivia study is part of an ongoing multiple case study organized by the FAO's Regional Office for Latin America and the
Caribbean as part of the activities of the Technical Cooperation Network on Agricultural Biotechnology (REDBIO/FAO). The
basic information was obtained through a survey and/or direct interview to a significant number of experts from Bolivian
institutions involving management, regulation, research, innovation, application and education of agricultural biotechnology. At
national level, the most relevant applied agri-biotechnologies are presently in vitro plant culture, genomics, molecular genetics,
assisted reproduction techniques in animals, and techniques supporting plant and animal disease diagnosis. They have been
developed and...
Tipo: Journal article
Palavras-chave: Andean crops; Appropriate biotechnology; Biotechnology administration; Bolivia; Genetic resources; Policy;
REDBIO/FAO; Regulations; Technical cooperation network on plant biotechnology.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100002
Avanços biotecnológicos na agricultura mundial.
Provedor de dados: 119
Autores: PAIVA, E.; CARNEIRO, N. P.
2000
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Agricultura; Mundial; Biotecnologia; Agricultural; Biotechnology; World.
Ano: 2000
Selecting regenerable maize genotypes with high activity of the gamma-zein gene promoters.
Provedor de dados: 119
Autores: LOPES, M.A.; VASCONCELOS, M. J. V.; ANTUNES, M.S.; BARDUCHE, D.; CARVALHO, M.E.; PAIVA, E.
1998
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; Zea mays; Maize; Biotechnology.
Ano: 1998
Seed germination inhibition of angico vermelho [Anadenanthera peregrina (Benth.) Speg.] by
desication and ABA treatment.
Provedor de dados: 119
Autores: BARDUCHE, D.; PAIVA, R.; ANDREOLI, R.; PAIVA, E.
1995
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Biotecnologia; Sementes; Angico; Biotechnology; Seed; Anadenanthera peregrina.
Ano: 1995
Avaliação de procedimentos de isolamento e purificação de DNA de milho (Zea mays L.).
Provedor de dados: 119
Autores: SOUZA JUNIOR, M.T.; PAIVA, E.
Foram avaliados 5 procedimentos de isolamento e purificacao de DNA genomico de milho. O procedimento A, que utiliza Nlauroylsarcosine e Proteinase K, mostrou-se como o que mais se aproxima do ideal, porem, apresenta a utilizacao e 1 mg de
Proteinase K por amostras como fator limitante. O uso de uma proteinase de amplo espectro se mostrou necessario ao processo de
purificacao de DNA.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Milho; Biotecnologia; DNA; Zea mays; Maize; Biotechnology.
Ano: 1992
Axillary shoot induction of mahogany (Swietenia macrophylla King).
Provedor de dados: 119
Autores: PINTO, F.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M. G. G.
2013
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Swietenia macrophylla; Mogno; Biotecnologia; Mahogany; Biotechnology; Chlorosis.
Ano: 2013
Proceedings...
Provedor de dados: 119
Autores: THE PLANT BIOTECHNOLOGY AND BIOSAFETY WORKSHOP, 2014, Brasília, DF.
2014
Tipo: Outras publicações científicas (ALICE)
Palavras-chave: Scientific Researches; Agricultural Biotechnology; Biosafety; Laws and regulations.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1012859
Riscos e beneficios.
Provedor de dados: 119
Autores: GAZZONI, D.L.
2001
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Agricultura; Transgenicos; Biotecnologia; Agriculture; Transgenics; Biotechnology.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/handle/doc/462663
Milho transgênico.
Provedor de dados: 119
Autores: CARNEIRO, A. A.; CARNEIRO, N. P.; CARVALHO, C.H.S.; VASCONCELOS, M. J. V.; PAIVA, E.; LOPES,M.A.
2000
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Milho transgenico; Biotecnologia; Transgenic maize; Biotechnology.
Ano: 2000
Effectiveness of both synthetic compounds and biological extracts against powdery mildew (Blumeria
graminis f. sp. tritici) on winter wheat
Provedor de dados: 115
Autores: Věchet,Lubomír; Šerá,Božena.
The objective of this study was to evaluate the effectiveness of foliar application of various synthetic compounds and biological
extracts against powdery mildew on wheat. Seed coats of winter wheat were treated by synthetical compounds (benzothiadiazole,
salicylic acid, and glycine betaine) or by the extracts from plants (oak, giant knotweed, curcuma, and ginger). Then the seeds were
planted using small-field experiments with powdery mildew infection (repetitions over a two-year period; experimental field in
Ruzyne, Prague). Leaf area infection was measured and the Cumulative Proportion of Leaf Area Diseased (CPLAD) was
calculated. Data were analysed using ANOVA (Tukey test, p≤0.05). Effectiveness of preparations varied 30 % to 72
% in 2008, and...
Tipo: Journal article
Palavras-chave: Biotechnology; Curcuma; Giant knotweed; Ginger; Infection; Oak.
Ano: 2015
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000100006
Intellectual property rights related to the genetically modified glyphosate tolerant soybeans in Brazil
Provedor de dados: 42
Autores: Rodrigues,Roberta L; Lage,Celso L. S; Vasconcellos,Alexandre G.
The present work analyzes the different modalities of protection of the intellectual creations in the biotechnology agricultural
field. Regarding the Brazilian legislations related to the theme (the Industrial Property Law - no. 9. 279/96 and the Plant Variety
Protection Law - no. 9. 456/97), and based in the international treaties signed by Brazil, the present work points to the inclusions
of each of them, as well as to their interfaces using as reference the case study of glyphosate tolerant genetically modified
soybean. For this case study, Monsanto's pipelines patents were searched and used to analyze the limits of patent protection in
respect to others related to the Intellectual Property (IP) laws. Thus, it was possible to elucidate the complex...
Tipo: Journal article
Palavras-chave: Biotechnology; GMO; Intellectual property; Patent; Pipeline; Soybean.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200029
Synthetic Biology: opportunities for Chilean bioindustry and education
Provedor de dados: 48
Autores: Federici,Fernán; Rudge,Timothy J; Pollak,Bernardo; Haseloff,Jim; Gutiérrez,Rodrigo A.
In an age of pressing challenges for sustainable production of energy and food, the new field of Synthetic Biology has emerged as
a promising approach to engineer biological systems. Synthetic Biology is formulating the design principles to engineer
affordable, scalable, predictable and robust functions in biological systems. In addition to efficient transfer of evolved traits from
one organism to another, Synthetic Biology offers a new and radical approach to bottom-up engineering of sensors, actuators,
dynamical controllers and the biological chassis they are embedded in. Because it abstracts much of the mechanistic details
underlying biological component behavior, Synthetic Biology methods and resources can be readily used by interdisciplinary
teams to...
Tipo: Journal article
Palavras-chave: Synthetic biology; Bioengineering; Education; Engineering; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400010
Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures
Provedor de dados: 52
Autores: Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants,
explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA
and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an
increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and
haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a
constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to...
Tipo: Journal article
Palavras-chave: Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020
Bark Extract of Bathysa cuspidata in the Treatment of Liver Injury Induced by Carbon
Tetrachloride in Rats
Provedor de dados: 52
Autores: Gonçalves,Reggiani Vilela; Matta,Sérgio Luis Pinto da; Novaes,Rômulo Dias; Leite,João Paulo Viana; Peluzio,Maria
do Carmo Gouveia; Vilela,Emerson Ferreira.
The aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic
parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of
CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for
another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or
400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity.
In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high
tissue content of...
Tipo: Journal article
Palavras-chave: Antioxidant defense; Biotechnology; Hepatotoxicity; Phytotherapy; Pathology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400504
Patent application in biotechnology at subclass C12N in Brazil at the period of 2001 to 2005
Provedor de dados: 52
Autores: Buttow,Mônica Elisa; Steindel,Mário.
An analysis of Instituto Nacional de Propriedade Industrial (INPI) patent database from 2001 to 2005 showed that less than 5% of
the total patent deposited in the subclass C12N, which referred to "Microorganisms or Enzymes, Compositions thereof,
Propagating, Preserving or Maintaining Microorganisms, Mutation or Genetic Engineering, Culture Media", were from national
depositors. In contrast, more than 34% of all these deposits came from the United States of America during the same period.
Among the national depositors, public universities and government research institutions contributed with 83% over all the
deposits, demonstrating that research and development in the field of Biotechnology in Brazil has been concentrated within
Brazilian public...
Tipo: Journal article
Palavras-chave: Patents; Biotechnology; Intellectual Property; Technologic Innovation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300003
A conceptual and practical overview of cDNA microarray technology: implications for basic and
clinical sciences
Provedor de dados: 56
Autores: Mello-Coelho,V. de; Hess,K.L.
cDNA microarray is an innovative technology that facilitates the analysis of the expression of thousands of genes simultaneously.
The utilization of this methodology, which is rapidly evolving, requires a combination of expertise from the biological,
mathematical and statistical sciences. In this review, we attempt to provide an overview of the principles of cDNA microarray
technology, the practical concerns of the analytical processing of the data obtained, the correlation of this methodology with other
data analysis methods such as immunohistochemistry in tissue microarrays, and the cDNA microarray application in distinct areas
of the basic and clinical sciences.
Tipo: Journal article
Palavras-chave: Biotechnology; CDNA microarray; Gene expression; Genomics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000011
Exploring the biotechnologial applications in the archaeal domain
Provedor de dados: 58
Autores: Alquéres,S.M.C.; Almeida,R.V.; Clementino,M.M.; Vieira,R.P.; Almeida,W.I.; Cardoso,A.M.; Martins,O.B.
Archaea represent a considerable fraction of the prokaryotic world in marine and terrestrial ecosystems, indicating that organisms
from this domain might have a large impact on global energy cycles. The extremophilic nature of many archaea has stimulated
intense efforts to understand the physiological adaptations for living in extreme environments. Their unusual properties make
them a potentially valuable resource in the development of novel biotechnological processes and industrial applications as new
pharmaceuticals, cosmetics, nutritional supplements, molecular probes, enzymes, and fine chemicals. In the present mini-review,
we show and discuss some exclusive characteristics of Archaea domain and the current knowledge about the biotechnological
uses of...
Tipo: Journal article
Palavras-chave: Archaea; Biotechnology; Extremozymes; Genomic; Molecular Phylogeny.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300002
Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea
[Cajanus cajan (L.) Millsp.]
Provedor de dados: 58
Autores: Fernandes Júnior,Paulo Ivan; Lima,Andréa Aparecida de; Passos,Samuel Ribeiro; Gava,Carlos Alberto Tuão;
Oliveira,Paulo Jansen de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro.
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt
and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the
majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated
at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very
diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total
of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The...
Tipo: Journal article
Palavras-chave: Strain selection; Biological nitrogen fixation; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400045
Influence of carbon source, pH, and temperature on the polygalacturonase activity of Kluyveromyces
marxianus CCMB 322
Provedor de dados: 116
Autores: Oliveira,Rodrigo de Queiroz; Valasques Junior,Gildomar Lima; Uetanabaro,Ana Paula Trovatti; Koblitz,Maria Gabriela
Bello; Góes-Neto,Aristóteles; Rosa,Carlos Augusto; Assis,Sandra Aparecida de.
Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. Two kinds
of yeast can be discerned regarding the production of enzymes. One group includes those which can produce enzymes in the
absence of an inducer, and the other group comprises the yeasts that produce enzymes in the presence of an inducer. The objective
of this study was to investigate the influence of pectic substances, glucose, pH, and temperature on the polygalacturonase activity
by Kluyveromyces marxianus CCMB 322. The yeast was grown in a fermentation broth containing different concentrations of
glucose and pectic substances. The polygalacturonase activity was determined by the DNS method, and the pH and temperature
were optimized...
Tipo: Journal article
Palavras-chave: Yeasts; Enzymes; Thermostability; Pectic substances; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300012
Island Communities and Biotechnology
Provedor de dados: 69
Autores: DaSilva,Edgar J.; Taylor,Mary.
In the run-up to the year 2000 and beyond, several island countries, especially in the Caribbean and Pacific regions, are confronted
by the challenges and threats of globalization. In response, several island countries are initiating self-reliant strategies aimed at
national and regional endogenous development. Amongst these strategies, the potential of biotechnology for economic
development and technological growth is being tapped.
Tipo: Journal article
Palavras-chave: Biotechnology; Pacific islands; Caribbean region; MIRCENS.
Ano: 1998
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100001
Transgenic trees and forestry biosafety
Provedor de dados: 69
Autores: Valenzuela,Sofía; Balocchi,Claudio; Rodríguez,Jaime.
The benefits from the development of transgenic trees are expected from the improvement of traits as growth and form, wood
quality, industrial processes, disease and insect resistance, herbicide tolerance, ecological restoration, rooting ability, etc. One of
the first reported field trials with genetically modified forest trees was established in Belgium in 1988 and the characteristic
evaluated was herbicide tolerance in poplars. Since then, there have been more than 200 reported trials, involving at least 15 forest
species. The majority of the field trials have been carried out in the USA (64%). More than 50% of the field trials are done with
Populus species and the main target traits are herbicide tolerance (31%), followed by marker genes (23%) and...
Tipo: Journal article
Palavras-chave: Biosafety; Forestry biotechnology; GMOs; GM trees.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300031
Knowledge about and acceptance of genetically modified organisms among pre-service teachers: a
comparative study of Turkey and Slovenia
Provedor de dados: 69
Autores: Sorgo,Andrej; Ambrozic-Dolinsek,Jana; Usak,Muhammet; Özel,Murat.
Genetically modified organisms cannot be regarded as merely a topic for academic debate, since these have serious implications
as a research field and for production based on genetic engineering. Public debates rarely base their arguments on elements rooted
in scientific arguments and knowledge but are heavily loaded with emotions, opinions and informal reasoning. This study aimed
to investigate the knowledge and acceptance of genetically modified organisms among prospective teachers in Slovenia and
Turkey. Knowledge of genetic modification was measured with a two-tier instrument. The level of acceptance of genetic
modification was measured with a 17-item instrument. Findings revealed that knowledge of genetics and biotechnology barely
influenced the...
Tipo: Journal article
Palavras-chave: Biotechnology; Genetic engineering; Genetic modifications; Teaching.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400005
The challenges of introducing a new biofungicide to the market: A case study
Provedor de dados: 69
Autores: Galindo,Enrique; Serrano-Carreón,Leobardo; Gutiérrez,Carlos Roberto; Allende,Raúl; Balderas,Karina; Patiño,Martín;
Trejo,Mario; Wong,Miguel Angel; Rayo,Emma; Isauro,Darío; Jurado,Carlos.
Background: Scientific literature contains many reports documenting the isolation and antagonist-testing of microorganisms with
the potential for biological control in agriculture, however, very few have addressed all aspects involved in the long process that
occurs before a potential strain, found and tested in laboratory, can reach commercialization. Results: After a multi-institutional
and multi-disciplinary effort to develop a biological control agent with remarkable technical characteristics and performance, the
fungicide Fungifree AB® was recently introduced in Mexican market. This product that has no chemical residues and is
environmentally friendly which can be used by mango, avocado, papaya and citrus fruits (and other crops in the short term)...
Tipo: Journal article
Palavras-chave: Biofungicides; Fungicides; Fungifree AB®; Process biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300005
Biosafety education relevant to genetically engineered crops for academic and non-academic
stakeholders in East Africa
Provedor de dados: 69
Autores: Sengooba,Theresa; Grumet,Rebecca; Hancock,James; Zawedde,Barbara; Kitandu,Lazaro; Weebadde,Cholani;
Karembu,Margaret; Kenya,Eucharia; Meredia,Karim; Nampala,Paul; Ochanda,James O; Quemada,Hector;
Rubindamayugi,Mugassa.
Development and deployment of genetically engineered crops requires effective environmental and food safety assessment
capacity. In-country expertise is needed to make locally appropriate decisions. In April 2007, biosafety and biotechnology
scientists, regulators, educators, and communicators from Kenya, Tanzania, and Uganda, met to examine the status and needs of
biosafety training and educational programs in East Africa. Workshop participants emphasized the importance of developing
biosafety capacity within their countries and regionally. Key recommendations included identification of key biosafety curricular
components for university students; collaboration among institutions and countries; development of informational materials for
non-academic...
Tipo: Journal article
Palavras-chave: Biotechnology; Environmental safety; Kenya; Tanzania; Uganda.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100001
Alternativas para a redução de custos na produção de mudas in vitro.
Provedor de dados: 14
Autores: RIBEIRO, J. M.; PINTO, M. dos S. T.; TEIXEIRA, S. L.
Este trabalho apresenta alternativas que podem contribuir para a redução de custos no processo de produção de mudas in vitro.
Substituição de componentes do meio nutritivo, substituição da autoclavagem pela esterilização química do meio nutritivo e
utilização de biorreatores de imersão temporária são algumas das alternativas abordadas na publicação. Trata-se de uma boa fonte
informação para aqueles que desejam ampliar seus conhecimentos acerca do tema, que deve ser amplamente discutido, pois é
aderente a um dos nossos maiores desafios: a produção de alimentos em complexos cenários climáticos
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Micropropagação; Cultura de tecido; Produção de mudas; Biotecnologia; Biotechnology.
Ano: 2013
Manual de biossegurança da Embrapa Soja.
Provedor de dados: 14
Autores: MARCELINO-GUIMARÃES, F. C.; ARIAS, C. A. A.; HUNGRIA, M.
Definição. Tipos de Risco. Riscos de acidentes. Riscos Físicos. Riscos químicos. Riscos biológicos. Classificação de risco
biológico. Níveis de contenção física para riscos biológicos. Classificação de risco biológico de organismos geneticamente
modificados (OGMs). Níveis de biossegurança para trabalhos com OGMs. Métodos de controle de agentes de risco.
Recomendações gerais. Barreiras de proteção. Procedimentos para descarte dos resíduos. Instalações físicas e procedimentos em
contenção para atividades e projetos com vegetais geneticamente modificados. Manuseio dos materiais GM. Rotinas de
esterilização. Anexo 1 - Procedimento para uso de cabines de biossegurança. Anexo 2 - Legislação referentes às atividades
descritas neste Manual.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biotecnologia; Biossegurança; Organismo transgênico; Biotechnology; Biosecurity.
Ano: 2014
Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high
lipid-accumulating mutant strains of Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret,
Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and
biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic
analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured
in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according
to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed
between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf
Review of advances in plant biotechnology, 1985-88: [proceedings]
Provedor de dados: 34
Autores: International Symposium on Genetic Manipulation in Crops (2nd : 1988 : Mexico).; International Rice Research
Institute.; Mujeeb-Kazi, A.; Sitch, L.A.
iv, 329 p. : ill., maps.
Tipo: Book
Palavras-chave: Genetic engineering; Biotechnology; Tissue culture; Plant breeding.
Ano: 1989
URL: http://hdl.handle.net/123456789/677
Breaking the yield barrier: proceedings of the workshop on rice yield potential in favorable
environments (1993 : IRRI).
Provedor de dados: 34
Autores: International Rice Research Institute.; Workshop on Rice Yield Potential in Favorable Environments (1993 : IRRI).
141 p. : ill.
Tipo: Book
Palavras-chave: Research; Yields; Constraints; Biotechnology; Genetic engineering; Ideotypes.
Ano: 1994
URL: http://hdl.handle.net/10269/283
The gene revolution
Provedor de dados: 34
Autores: Macintosh, D.
p.14-18
Palavras-chave: Genomes; Biotechnology; Research; Public sector; Private sector.
Ano: 2006
URL: http://hdl.handle.net/10269/40
Multivalent ligands of CD22 for targeting of B cells
Provedor de dados: 17
Autores: James C. Paulson.
Multivalent ligands of CD22 for active targeting of B cells. Mary O’Reilly, Wei Hsu Chen, Gladys Completo, Ying
Zeng, Satoshi Futakawa and Cory Rillahan and James C. Paulson, Departments of Chemical Physiology and Molecular Biology,
The
Scripps
Research
Institute,
10550
N.
Torrey
Pines
Road,
La
Jolla,
CA,
92037

The siglecs comprise 13 members of the immunoglobulin superfamily that
recognize sialic acid containing glycans, and are differentially expressed on leukocytes and glial cells.
The natural ligands of
siglecs typically occur on the same cell (in cis) and/or on adjacent cells (in trans). Cis ligands mask the binding of multivalent
synthetic sialoside ligands and are thought to regulate the activity of...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Molecular Cell Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3549/version/1
A Proposal of Genomic Analytical Workflow in a Bacterial Pathogen Outbreak Investigation
Provedor de dados: 17
Autores: Hoi Shan Kwan; Chun Hang Au; Chi Keung Cheng; Man Kit Cheung; Qianli Huang; Lei Li; Wenyan Nong; Man Chun
Wong.
The German Escherichia coli O104:H4 outbreak causing Hemolytic Uremic Syndrome (HUS) started in early May 2011.
Conventional typing methods including serotyping, multi-locus sequence typing (MLST) and pulse-field gel electrophoresis
(PFGE) seemed to yield only limited information about the causative agent. The advantages of the Genome sequencing approach
are apparent compared to conventional methods. Genome sequencing and analyses with an appropriate workflow would be the
new paradigm in bacterial outbreak investigation.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/6663/version/1
The biotechnological perspective of Beta-Glucosidases
Provedor de dados: 17
Autores: Israr Khan; Muhammad waheed Akhtar.
Biotechnology is the art and science of making and technology to process biological materials for the welfare of human being.
This discussion includes the beta glucosidase classification, its structure, application, and recent research interests in relation to its
significance and comparison with eukaryotic Beta glucosidases including plant, animal and yeast as well. The study also comprise
of the various stress factors and modifications thereby the organism to cope it. The focus of the study is on the various kinetics
principles and their full potential exploitation to increase the activity of the enzyme. As such making the enzyme a potent vehicle
for various industrial, pharmaceutical and research purposes. It also include the various glycosylhydrolases...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4945/version/1
CloVR-16S: Phylogenetic microbial community composition analysis based on 16S ribosomal RNA
amplicon sequencing – standard operating procedure, version1.0
Provedor de dados: 17
Autores: James R. White; Cesar Arze; Malcolm Matalka; The CloVR Team; Samuel V. Angiuoli; W. Florian Fricke.
The CloVR-16S pipeline employs several well-known phylogenetic tools and protocols for the analysis of 16S rRNA sequence
datasets:

A) Mothur [1] – a C++ - based software package used for
clustering 16SrRNA sequences into operational taxonomic units (OTUs). Mothur creates OTUs using a matrix that describes
pairwise distances between representative aligned sequences and subsequently estimates within-sample diversity (alpha
diversity);
B)The Ribosomal Database (RDP) naive Bayesian classifier [2] assigns each 16S sequence to a
reference taxonomy with associated empirical probabilities based on oligonucleotide frequencies;
C) Qiime
[3] – a python-based workflow package,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5888/version/1
Bogoch Replikins Pandemic Prevention: Increase of Strain-Specific Influenza Genomic Replikin
Counts, Having Predicted Outbreaks and their Location Seven Times Consecutively, Up to Two Years
in Advance, Provides Time for Prevention of Pandemics
Provedor de dados: 17
Autores: Samuel
Bogoch; Elenore S. Bogoch.
Earlier studies have shown that the increased concentration of a new class of virus genomic peptides, Replikins, precedes and
predicts virus outbreaks. We now find that the area in the genome of the highest concentration of Replikins, and the country in
which this peak exists in scout viruses, have permitted in the past five years seven consecutive accurate predictions of the
geographic localization of coming outbreaks, including those now realized in Mexico for H1N1, and in Cambodia for H5N1.
Real-time Replikin analysis of the evolution of the virus genome identified both mutations and structural reorganization of the
hemagglutinin and p B1 genes over several years before each outbreak. This information, together with the specific Replikin
sequences so...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6952/version/1
Reversal of an immunity associated plant cell death program by the growth regulator auxin
Provedor de dados: 17
Autores: Suresh Gopalan.
One form of plant immunity against pathogens involves a rapid host programmed cell death at the site of infection accompanied
by resistance, termed the hypersensitive response (HR). Here it is shown that the HR programmed cell death program initiated by
the bacterial type III secretion system dependent proteinaceous elicitor harpin from Erwinia amylovora can be reversed till very
late in the process by the plant growth regulator auxin. Early inhibition or late reversal of this cell death program does not affect
marker genes tightly correlated with local and systemic resistance. Cross-regulation between cell death programs and growth
regulators is prevalent in different kingdoms. Thus, the concept that cell death program can be reversed till late provides a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology; Plant Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1347/version/1
Successful use of axonal transport for drug delivery by synthetic molecular vehicles
Provedor de dados: 17
Autores: Aaron G. Filler; Garth Whiteside; Mark Bacon; Martyn Frederickson; Franklyn A. Howe; Miri K. Rabinowitz; Alan J.
Sokoloff; Terrence Deacon; Chris Abell; Raj Munglani; John R. Griffiths; B. Anthony Bell; Andrew Lever.
We report the use of axonal transport to achieve intraneural drug delivery. We constructed a novel tripartite complex of an axonal
transport facilitator conjugated to a linker molecule bearing up to a hundred reversibly attached drug molecules. The complex
efficiently enters nerve terminals after intramuscular or intradermal administration and travels within axonal processes to neuron
cell bodies. The tripartite agent provided 100-fold amplification of saturable neural uptake events, delivering multiple drug
molecules per complex. _In vivo_, analgesic drug delivery to systemic and to non-targeted neural tissues was greatly reduced
compared to existing routes of administration, thus exemplifying the possibility of specific nerve root targeting and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2164/version/1
Allelic Frequency of ABO And Rh D Blood Group Among The Banjara Caste Population of Akola
District, Maharashtra , India
Provedor de dados: 17
Autores: Aravind B. Chavhan.
The distribution of ABO blood groups and Rh (D) factor has been studied among the Banjara Caste population of Akola District
(Maharashtra). The O, A, B, and AB blood group percentage were recorded as 27.64%, 22.91%, 37.45% and 12% respectively.
The Rh negative incidence was recorded as 02.55%. The allele frequencies of O, A, B and AB groups in the combined data were
found to be 0.5196, 0.2880, and 0.1924 respectively and Rh (D) positive allelic frequency was 0.8405.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5771/version/1
CloVR-Microbe: Assembly, gene finding and functional annotation of raw sequence data from single
microbial genome projects – standard operating procedure, version 1.0
Provedor de dados: 17
Autores: Kevin Galens; James R. White; Cesar Arze; Malcolm Matalka; Michelle Gwinn Giglio; The CloVR Team; Samuel V.
Angiuoli; W. Florian Fricke.
The CloVR-Microbe pipeline performs the basic processing and analysis steps required for standard microbial single-genome
sequencing projects: A) Whole-genome shotgun sequence assembly; B) Identification of protein and RNA-coding genes; and C)
Functional gene annotation. B) and C) are based on the IGS Annotation Engine (http://ae.igs.umaryland.edu/), which is described
elsewhere (K Galens et al. submitted). The assembly component of CloVR-Microbe can be executed independently from the gene
identification and annotation components. Alternatively, pre-assembled sequence contigs can be used to perform gene
identifications and annotations. The pipeline input may consist of unassembled raw sequence reads from the Sanger, Roche/454
GS FLX or Illumina GAII or...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5887/version/1
Flaw-tolerance in silk fibrils explains strength, extensibility and toughness of spider silk
Provedor de dados: 17
Autores: Melis Arslan; Markus J. Buehler.
Silk is an ancient but remarkably strong, extensible and tough material made from simple protein building blocks. Earlier work
has shown that the particular molecular geometry of silk with a composite of semi-amorphous and nanocrystalline beta-sheet
protein domains provides the structural basis for its characteristic softening-stiffening behavior and remarkable strength at the
nanoscale. Yet, an open question remains as to how these nanoscale properties are upscaled so effectively to create strong,
extensible and tough silk fibers. Here we discover that the geometric confinement of fibrils to ≈50-100 nm width
and arranged in bundles to form larger-scale silk fibers, is the key to explaining the upscaling of the mechanical properties of silk
from...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics; Earth & Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5916/version/1
Characterization of a pig skeletal muscle microarray to study pork quality: the GenmascqChip 15K
Provedor de dados: 17
Autores: Marie Damon; Frédéric Herault; Annie Vincent; Pascale Le Roy; Pierre Cherel.
Pork quality is highly variable and availability of relevant predictors of fresh meat quality at slaughter is critical to optimize
carcass use and improve its valuation. High throughput gene expression studies have been widely used to describe biological
mechanisms underlying variation of several traits but data are scarce regarding their use as tools for development of biomarkers.
The GENMASCQ (GENomics enabled Marker Assisted Selection and Certification of Quality in Pork products ) research
program was set up to select, using high-throughput gene expression screening, markers of the biological variation mostly
appropriate to describe, and ultimately forecast, the sensory quality of an important meat product, accounting for nearly 40 % of
meat protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5730/version/1
Acute Toxicity of Perfluorooctane Sulfonic Acid on Early Life Stages of Daphnia Magna, Siriella
Armata, Paracentrotus Lividus Sea Urchin, and Turbot (Scophthalmus Maximus L.).
Provedor de dados: 17
Autores: Lazhar Mhadhbi; Sara Pérez Fernandez; Diego Real Conde; Moncef Boumaiza; Ricardo Beiras.
Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant whose potential toxicity is causing great concern. In the present
study, we used two crustaceans (_Daphnia magna_, _Siriella armata_), echinoid embryos (_Paracentrotus lividus_) and turbot
(_Scophthalmus maximus L._) embryos and larvae to investigate the acute toxicity of this compound. The marine species _S.
armata_ exhibited greater sensitivity than the freshwater species _D. magna_. In the 48-h acute toxicity test the median lethal
concentration was 10.96 mg/L for _S. armata_ and 87.46 mg/L for _D. magna_. In the 96h toxicity test, turbot showed the lowest
EC50 (0.155 mgL-1), whilst the EC50 for _S. armata_ was 7.92 mgL-1 which was approximately three times lower than that for
sea urchin...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Ecology; Earth & Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4657/version/1
Artificial Photosynthesis Would Unify the Electricity-Carbohydrate-Hydrogen Cycle for
Sustainability
Provedor de dados: 17
Autores: Yi-Heng Percival Zhang.
Sustainable development requires balanced integration of four basic human needs – air (O2/CO2), water, food, and
energy. To solve key challenges, such as CO2 fixation, electricity storage, food production, transportation fuel production,
water conservation or maintaining an ecosystem for space travel, we wish to suggest the electricity-carbohydrate-hydrogen
(ECHo) cycle, where electricity is a universal energy carrier, hydrogen is a clean electricity carrier, and carbohydrate is a
high-energy density hydrogen (14.8 H2 mass% or 11-14 MJ electricity output/kg)carrier plus a food and feed source. Each
element of this cycle can be converted to the other reversibly & efficiently depending on resource availability, needs,
and costs. In...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Earth & Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4167/version/1
Human Cancer Cells Express Slug-based Epithelial-Mesenchymal Transition Gene Signature
Obtained in Vivo
Provedor de dados: 17
Autores: Dimitris Anastassiou; Viktoria Rumjantseva; Wei-yi Cheng; Jianzhong Huang; Peter D. Canoll; Darrell J. Yamashiro;
Jessica J. Kandel.
The biological mechanisms underlying cancer cell motility and invasiveness remain unclear, although it has been hypothesized
that they involve some type of epithelial-mesenchymal transition (EMT). Here we show that human cancer cells express in vivo a
precise multi-cancer invasion-associated gene expression signature characterized by the prominent presence of collagen
COL11A1 and thrombospondin THBS2. The signature is present in the expression of all solid tumor datasets that we analyzed and
includes numerous EMT markers, among them the transcription factor Slug, fibronectin, and α SMA. Using xenograft
models of human cancer cells in immunocompromised mice and profiling the harvested tumors separately with species specific
probes, we found that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6243/version/1
Study of Fish Weight Loss in Solar Dryer Across Different Agro-Ecological Zones of Nigeria
Provedor de dados: 17
Autores: Julius Olokor; Emeka Ugoala; Adenike Ibitoye.
Drying sessions were conducted across Nigeria to study the interaction between fish weight loss and the meteorological
parameters. Correlation analyses using weight loss values show that latitude is strongly related (r =+0.96) to weight loss of fish
inside the dryer while altitude had a weak relationship (r = +0.24) with weight loss. The results show that New Bussa recorded
more weight loss of fish than Jos, despite their uniform latitude. Weight loss records in Jos, was however better than those of
Warri and Ibadan.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth & Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/3018/version/1
Extracting Functional Modules from Biological Pathways
Provedor de dados: 17
Autores: Sihai D. Zhao; Yong Li.
It has been proposed that functional modules are the fundamental units of cellular function. Methods to identify these modules
have thus far relied on gene expression data or protein-protein interaction (PPI) data, but have a few limitations. We propose a
new method, using biological pathway data to identify functional modules, that can potentially overcome these limitations. We
also construct a network of these modules using functionally relevant PPI data. This network displays the flow and integration of
information between modules and can be used to map cellular function.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1457/version/1
A subset of co-expressed genes in Slug-based cancer mesenchymal transition signature remains
coexpressed in normal samples in a tissue-specific manner
Provedor de dados: 17
Autores: Weiyi Cheng; Dimitris Anastassiou.
A recently identified gene expression signature of EMT markers containing the transcription factor Slug was found present in
samples from many publicly available cancer gene expression datasets of multiple cancer types except leukemia. We also found
many of these genes co-expressed in human cancer xenografted cells, but not in mouse stroma cells, suggesting that the signature
is largely produced by cancer cells undergoing some type of EMT. Here we report that a partial signature consisting of a subset of
the co-expressed genes of the full signature, including at least Slug (SNAI2), collagens COL1A1, COL1A2, COL3A1, COL6A3
and genes DCN and LUM, is also present in leukemia, in which case it is also strongly associated with the chemokine CXCL12
(aka SDF1)....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6813/version/1
Genetic incorporation of D-Lysine into diketoreductase in Escherichia coli cells
Provedor de dados: 17
Autores: Zhi Zhi Liu; Xin Yang; Denghuan Yi; Shuzhen Wang; Yijun Chen.
D-Lysine has been genetically introduced into diketoreductase in E. coli cells by utilization of an orthogonal Ph tRNA
/Lysyl-tRNA synthetase pair. This is the first report on the genetic incoporation of D-amino acids into proteins, which may be
generally applicable to a wide variety of applications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology; Molecular Cell Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6777/version/1
ProbCD: enrichment analysis accounting for categorization uncertainty
Provedor de dados: 17
Autores: Ricardo Vêncio; Ilya Shmulevich.
As in many other areas of science, systems biology makes extensive use of statistical association and significance estimates in
contingency tables, a type of categorical data analysis known in this field as enrichment (also over-representation or enhancement)
analysis. In spite of efforts to create probabilistic annotations, especially in the Gene Ontology context, or to deal with uncertainty
in high throughput-based datasets, current enrichment methods largely ignore this probabilistic information since they are mainly
based on variants of the Fisher Exact Test. We developed an open-source R package to deal with probabilistic categorical data
analysis, ProbCD, that does not require a static contingency table. The contingency table for
the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/369/version/1
High-Throughput Transcriptome Profiling Of Human Mesenchymal Stem Cells Reveals A Role For
Wnt/GSK-3 Signaling In Their Hypoimmunomodulation
Provedor de dados: 17
Autores: Gurudutta Gangenahalli; Neeraj Satija; Farhat Afrin; Rajender Prasad Tripathi.
Mesenchymal stem cells (MSCs) are gaining importance among clinicians following recent demonstration of safe allogeneic
transplantation due to their ability to modulate the immune response. However,the molecular machinery regulating the expression
of immunomodulatory factors in MSCs is unknown. We, therefore, inhibited glycogen synthase kinase-3 (GSK-3),a Wnt signaling
inhibitor, to elucidate the role of Wnt signaling in mediating immunoregulatory effects of human MSCs using gene expression
profiling. Our results highlight enhanced ability of GSK-3 inhibitor (lithium) treated MSCs to evade immune response as a result
of decreased expression of immune stimulatory cytokines and chemokines.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5512/version/1
Emergence and Fixing of Antiviral Resistance in Influenza A Via Recombination and Hitch Hiking
Provedor de dados: 17
Autores: Henry L. Niman.
The dramatic rise of oseltamivir resistance in the H1N1 serotype in the 2007/2008 season and the fixing of H274Y in the
2008/2009 season has raised concerns regarding individuals at risk for seasonal influenza, as well as development of similar
resistance in the H5N1 serotype. Previously, oseltamivir resistance produced changes in H1N1 and H3N2 at multiple positions in
treated patients.
In contrast, the recently reported resistance involved patients who had not recently taken oseltamivir.
Moreover, the resistance was limited to the H1N1 which had acquired H274Y. Using phylogenetic analysis I show that the
fixing of H274Y was due to hitch hiking on a genetic background that acquired key changes from another circulating sub-clade.
H274Y jumped from...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology.
Ano: 2009
URL: http://precedings.nature.com/documents/2832/version/1
BioenergyProduction From Plant Biomass: Bioethanol From Concept To Reality
Provedor de dados: 17
Autores: Israr Khan; Muhammad Waheed Akhtar.
Natural energy reserves like petrol and diesel etc are depleting. As such, it is of paramount importance to find alternative fuels.
Solar energy is trapped as biomass in plants. Biomass provide an excellent reserve for trapping solar energy on field. Complex
enzymatic machinery comprising of hydrolytic reactions and consolidated bioprocessing is needed to make viable bioenergy,
mostly biofuels like bioethanol for locomotives, industries and general use in the future.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6286/version/1
Towards a genome-wide transcriptogram: the Saccharomyces cerevisiae case
Provedor de dados: 17
Autores: José Luiz Rybarczyk; Mauro A. A. Castro; Rodrigo J. S. Dalmolin; José C. F. Moreira; Leonardo
G. Brunnet; Rita M. C. de Almeida.
A genome modular classification that associates cellular processes to modules could lead to a method to quantify the differences
in gene expression levels in different cellular stages or conditions: the transcriptogram, a powerful tool for assessing cell
performance, would be at hand. Here we present a computational method to order genes on a line that clusters strongly interacting
genes, defining functional modules associated with gene ontology terms. The starting point is a list of genes and a matrix
specifying their interactions, available at large gene interaction databases. Considering the Saccharomyces cerevisiae genome we
produced a succession of plots of gene transcription levels for a fermentation process. These plots discriminate the fermentation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3869/version/1
Direct fluorophore conjugation to genomic DNA for microarray-based epigenomic profiling
Provedor de dados: 17
Autores: Vineet Gupta.
A methodology for microarray based epigenomic profiling is presented. The method relies on platinum-based fluorescence
labeling reagents for direct (non-enzymatic) labeling of DNA and RNA. This is a work in progress and only preliminary data is
presented here.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/444/version/1
Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants
Provedor de dados: 17
Autores: Hany A. El-Shemy; Mutasim M. Khalafalla; Masao Ishimoto.
The green fluorescent protein (GFP) of jellyfish (_Aequorea victoria_) has significant advantages over other reporter genes,
because expression can be detected in living cells without any substrates. Recently, epigenetic phenomena are important to
consider in plant biotechnology experiments for elucidate unknown mechanism. Therefore, soybean immature cotyledons were
generated embryogenesis cells and engineered with two different gene constructs (pHV and pHVS) using gene gun method. Both
constructs contain a gene conferring resistance to hygromycin (_hpt_) as a selective marker and a modified glycinin (11S
globulin) gene (_V3-1_) as a target. However, sGFP(_S65T_) as a reporter gene was used only in pHVS as a reporter gene for
study the relation between...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Plant Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1845/version/1
Paraoxonase 1 Polymorphism p.Q192R in Patients With Dementia
Provedor de dados: 17
Autores: Tomasz Krzywkowski.
PON1 PCR-RFLP polymorphism frequency and enzyme activity were determined in 223 patients with dementia (94 with AD, 55
with VaD and 74 with MD) and in 100 age and sex matched controls without dementia. I found no statistical significance of
genotype frequencies between analyzed groups. 
Paraoxonase 1 activity was lower in patients carrying the R
allele in locus 192 as compared with the R allele non-carriers. The results showed statistically significant association between
PON1 polymorphism and enzyme activity and thus can suggest an important relationship between different isoforms of PON1,
enzyme activity and dementia.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Neuroscience.
Ano: 2011
URL: http://precedings.nature.com/documents/6333/version/1
Optogenetic control of genetically-targeted pyramidal neuron activity in prefrontal cortex
Provedor de dados: 17
Autores: Michael V. Baratta; Shinya Nakamura; Peter Dobelis; Matthew B. Pomrenze; Samuel D. Dolzani; Donald C. Cooper.
A salient feature of prefrontal cortex organization is the vast diversity of cell types that support the temporal
integration
routing
of
of
electrical
events
required
information
activity
for
among
sculpting future
prefrontal
responses.
neuronal
A
subtypes
major
is
the
obstacle
inability
and
in
to
understanding
the
manipulate
the
of genetically defined cell types over behaviorally relevant timescales and activity patterns. To address
these constraints, we present here a simple approach for selective activation of prefrontal excitatory
vitro
in
vivo
preparations.
Rat
prelimbic pyramidal neurons
were
genetically
neurons
in
targeted
to
both
in
express
a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/7102/version/1
CloVR-Metagenomics: Functional and taxonomic microbial community characterization from
metagenomic whole-genome shotgun (WGS) sequences – standard operating procedure,
version 1.0
Provedor de dados: 17
Autores: James R. White; Cesar Arze; Malcolm Matalka; The CloVR Team; Samuel V. Angiuoli; W. Florian Fricke.
The CloVR-Metagenomics pipeline employs several well-known tools and protocols for the analysis of metagenomic
whole-genome shotgun (WGS) sequence datasets:

A) UCLUST [1] – a
C++-based software package for clustering redundant DNA sequences and removing artificial 454 replicates [1];

B)
BLASTX
and
BLASTN
[2]
for
functional
and
taxonomic
assignment
of
sequences,
respectively;
C) Metastats [3] and custom R scripts to generate additional statistical and graphical
evaluation.

The CloVR-Metagenomics pipeline accepts as input multiple fasta files (1
sample per file) and a corresponding tab-delimited metadata file that specifies features associated...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5886/version/1
Nanoconfinement of spider silk fibrils begets superior strength, extensibility and toughness
Provedor de dados: 17
Autores: Tristan Giesa; Melis Arslan; Nicola Pugno; Markus J. Buehler.
Silk is an exceptionally strong, extensible and tough material made from simple protein building blocks. The molecular structure
of dragline spider silk repeat units consists of semi-amorphous and nanocrystalline beta-sheet protein domains. Here we show by a
series of computational experiments how the nanoscale properties of silk repeat units are scaled up to create macroscopic silk
fibers with outstanding mechanical properties despite the presence of cavities, tears and cracks. We demonstrate that the
geometric confinement of silk fibrils to diameters of 50±30 nm width is critical to facilitate a powerful mechanism by
which hundreds of thousands of protein domains synergistically resist deformation and failure to provide enhanced strength,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics; Earth & Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5916/version/2
Weight matrix based identification of terpene synthases conserved motifs in Arabidopsis thaliana
proteome
Provedor de dados: 17
Autores: Suchita Srivastava; Suaib Luqman; Feroz Khan.
Terpenes comprise the most diverse collection of natural products. Out of more than 30,000 individual terpenoids identified, at
least half are synthesized by plants. A relatively small, but quantitatively significant, number of terpenoids are involved in primary
plant metabolism. However, the vast majorities are classified as secondary metabolites; compounds not required for plant growth
and development but presumed to have an ecological function in communication or defense and are widely used in industrial
applications. Terpene hydrocarbon scaffolds are generated by the action of the mechanistically intriguing family of mono-,
sesqui-, and diterpene synthases collectively termed as terpene synthases, that catalyze multistep reactions with
diphosphorylated...
Tipo: Poster
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6909/version/1
A Simple, Rapid Method for Extracting Large Plasmid DNA from Bacteria
Provedor de dados: 17
Autores: Spencer D. Heringa; Jonathan D. Monroe; James B. Herrick.
We are studying the lateral transfer of transmissible antibiotic resistance plasmids among stream bacteria impacted by fecal runoff
from poultry and cattle. Such plasmids are typically large (ca. 40 – 100 kb) and occur in low copy numbers in the
cell and have therefore typically been difficult to isolate and therefore to study. Traditional protocols, based upon variations of the
standard alkaline-lysis method, are long (ca. 1 1/2 to 2 days) and difficult. Commercial kits designed for the isolation of Baterial
Artificial Chromosomes (BACs) can be used and are an improvement; however, these are expensive and still require hours of
sustained effort. We have adapted a method published by Rondon et al. (1999), originally designed for the isolation of...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1249/version/1
Connecting Seed Lists of Mammalian Proteins Using Steiner Trees
Provedor de dados: 17
Autores: Amelia G. White; Avi Ma'ayan.
Multivariate experiments and genomics studies applied to mammalian cells often produce lists of genes or proteins altered under
treatment/disease vs. control/normal conditions. Such lists can be identified in known protein-protein interaction networks to
produce subnetworks that “connect” the genes or proteins from the lists. Such subnetworks are
valuable for biologists since they can suggest regulatory mechanisms that are altered under different conditions. Often such
subnetworks are overloaded with links and nodes resulting in connectivity diagrams that are illegible due to edge overlap. In this
study, we attempt to address this problem by implementing an approximation to the Steiner Tree problem to connect seed lists of
mammalian...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1768/version/1
Structure and function prediction of human homologue hABH5 of _E. coli_ ALKB5 using in silico
approach
Provedor de dados: 17
Autores: Mr. Shankaracharya; Saibal Das; Ambarish Sharan Vidyarthi.
Newly discovered human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer
therapy. Little is known about the structure and function of hABH5, one of the members of this superfamily. Therefore, in the
present study we intend to predict its structure and function using various bioinformatics tools. Modeling was done with modeler
9v7 to predict the 3D structure of the hABH5 protein. 3-D model of hABH5, ALKBH5.B99990005.pdb was predicted and
evaluated. Validation results showed 96.8% residues in favor and an additional allowed region of the Ramachandran plot. Ligand
binding residues prediction showed four ligand clusters, having 25 ligands in cluster 1. Importantly, conserved pattern of
Pro158-X-Asp160-Xn-His266 in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5597/version/2
PharmGKB: Capturing knowledge to catalyze pharmacogenomics research
Provedor de dados: 17
Autores: Russ B. Altman.
This is a powerpoint presentation made initially at the Cold Spring Harbor meeting on Pharmacogenomics, November 2006. It
discusses the PharmGKB (http://www.pharmgkb.org/) and how we are building a pipeline for annotation of knowledge. In
particular, we are focusing on pathway knowledge, "Very important Pharmacogene" annotations, and
curation of the pharmacogenomics literature. We are building an ontology-based system to capture and aggregate knowledge, for
use by our curators.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Pharmacology.
Ano: 2006
URL: http://precedings.nature.com/documents/8/version/1
Tracing blastomere fate choices of early embryos in single cell culture
Provedor de dados: 17
Autores: Yunhan Hong; Haobin Zhao; Ni Hong; Zhendong Li; Meisheng Yi; Rong Liu; Mingyou Li; Yan Yan; Yongming Yuan;
Chang Ming Li; Ruowen Ge; Jianxin Song.
Blastomeres of early vertebrate embryos undergo numerous fate choices for division, motility, pluripotency maintenance and
restriction culminating in various cell lineages. Tracing blastomere fate choices at the single cell level in vitro has not been
possible because of the inability to isolate and cultivate early blastomeres as single cells. Here we report the establishment of
single cell culture system in the fish medaka, enabling the isolation and cultivation of individual blastomeres from 16- to 64-cell
embryos for fate tracing at the single cell level in vitro. Interestingly, these blastomeres immediately upon isolation exhibit
motility, lose synchronous divisions and even stop dividing in ≥50% cases, suggesting that the widely accepted...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4907/version/1
The Molecular In Silico Dissection of Cancer using TCLASS and its Application in the Identification
of the Tissue of Origin of Cancers of Unknown Primary Site
Provedor de dados: 17
Autores: Torik Ayoubi.
Traditional cancer diagnosis relies on a combination of clinical and histo-pathological data. In many cases, however, the
morphology of in particular metastatic cancer is not discriminative enough for correct diagnosis. In addition, the morphology of
tumor cells do not provide information on aberrant molecular pathways that may be used to specifically target the tumor like the
application of Herceptin to breast cancers with amplification in the ERBB2 gene. With the completion of the human genome
project, molecular pathology has dramatically increased its arsenal of novel molecular biomarkers that can be used to determine
the phenotype of tumors. Gene expression profiling using high-density microarray technology is an extremely powerful tool to...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5300/version/1
Hierarchical coexistence of universality and diversity controls robustness and multi-functionality in
intermediate filament protein networks
Provedor de dados: 17
Autores: Theodor Ackbarow; Markus J. Buehler.
Proteins constitute the elementary building blocks of a vast variety of biological materials such as cellular protein networks, spider
silk or bone, where they create extremely robust, multi-functional materials by self-organization of structures over many lengthand time scales, from nano to macro. Some of the structural features are commonly found in a many different tissues, that is,
they are highly conserved. Examples of such universal building blocks include alpha-helices, beta-sheets or tropocollagen
molecules. In contrast, other features are highly specific to tissue types, such as particular filament assemblies, beta-sheet
nanocrystals in spider silk or tendon fascicles. These examples illustrate that the coexistence of universality and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/826/version/1
Technical Comments on “Greater Transportation Energy and GHG Offsets from
BioelectricityThan Ethanol”
Provedor de dados: 17
Autores: Y-H Percival Zhang; Joe Rollin.
Campbell et al. [Report in Science 2009 (May 22), 324, 1055] analyzed two scenarios where bioenergy crops were used for
transportation – (1) cellulosic ethanol production by biorefineries for internal combustion engines and (2)
bioelectricity generation by power stations for electric motors. Their study suggests the latter approach offers more efficient
energy utilization than the former. We argue that the evaluation of a transportation system must be based on complicated metrics
rather than only one or two factors. We suggest another out-of-the-box solution biomass carbohydrate-to-hydrogen-to-electricity
as a key component of the future transportation sector.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Earth & Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/3725/version/1
Biomimetic assemblage of nucleobase 5-fluorouracil into nano-size three-dimensional particles
Provedor de dados: 17
Autores: Arun Chauhan; Swaleha Zubair; Qamar Zia; Saba Tufail; Asif Sherwani; Mohammad Sajid; Mohammad Owais.
Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple
components with sub-nanometer precision. Recently, extract from cells of microorganism/plant origin have emerged as a simple
and viable route for the synthesis of metal and inorganic material nano-particles. In the present study, we have extended biological
synthesis of nano-particles to organic molecules, namely the anticancer agent, 5-fluorouracil (5-FU) using Aloe vera leaf extract.
The formation of 5-FU nano- particles was assessed by UV, FT-IR and fluorescence spectroscopic techniques. Transmission
electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6182/version/1
Control in technological systems and physical intelligence: an emerging theory
Provedor de dados: 17
Autores: Bradly Alicea.
The transduction and processing of physical information is becoming important in a range of research fields, from the design of
materials and virtual environments to the dynamics of cellular microenvironments. Previous approaches such as morphological
computation/soft robotics, neuromechanics, and embodiment have provided valuable insight. This work approaches haptic,
proprioception, and physical sensing as all part of the same subject. In this presentation, three design criteria for applying physical
intelligence to engineering applications will be presented. These criteria have several properties in common, which inspires two
types of end-effector model: stochastic (based on a spring) and deterministic (based on a piezomechanical array). The
generalized...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Neuroscience.
Ano: 2011
URL: http://precedings.nature.com/documents/5910/version/1
Development and comparison of ECC-rtPCR with in vivo method to determine the viability and
infectivity of Giardial cysts
Provedor de dados: 17
Autores: M. Khalid Ijaz; Absar Alum; Basel Sbai; Hamas Asaad; Joseph Rubino.
Globally, Giardia is one of the major cause of diarrheal illnesses and rapid diagnostic methods differentiating infectious cysts are
critical for developing intervention strategies through contaminated surfaces, food and water. This is currently hampered by lack
of an in vitro model. We evaluated mRNA expression in trophozoites and their attachment to a CaCo2 (C2bb) cell monolayer
and changes in trans-cellular resistance as an indicator of Giardia viability and infectivity. Heat shock mRNA in Giardia cysts
and variant specific protein (VSP) mRNA in trophozoites were quantified by RT-PCR. When compared with neonatal mice
infectivity, the attachment of trophozoites to cell monolayers, expression of VSP, and changes in the trans-cellular resistance,
the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Earth & Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5501/version/1
Genome Replikin Count Predicts Increased Lethality of Resistant Tuberculosis
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
The genomic Replikin Count of all the sequences on Pubmed of different strains of tuberculosis were analyzed. The lowest
Counts occurred with species within the lowest drug resistance, the highest Counts with sequences of the highest drug resistance
and lethality.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7141/version/1
Biotransformation of artemisinin mediated through fungal strains for obtaining derivatives with novel
activities
Provedor de dados: 17
Autores: Suchita Srivastava; Suaib Luqman; Atiya Fatima; Mahendra P. Darokar; Arvind S. Negi; J K. Kumar; Karuna
Shanker; Chandan S. Chanotiya; Sudeep Tandon; Suman P. S. Khanuja.
Artemisinin, a sesquiterpene lactone, is the active antimalarial constituent of Artemisia annua. Several fungal strains
Saccharomyces cerevisiae, Aspergillus flavus, Aspergillus niger and Picchia pastoris were used to biotransform artemisinin.
Among these strains, A. flavus was the only microorganism capable of transforming artemisinin to deoxyartemisinin in higher
yields than the previous reports. The structure of deoxyartemisinin was elucidated by spectroscopy. Deoxyartemisinin showed
antibacterial activity against Staphylococcus aureus, S. epidermidis and S. mutans at a minimum inhibitory concentration (MIC)
of 1 mg/mL compared to artemisinin whose MIC was >2 mg/mL.
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Microbiology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6925/version/1
The Modified Pharaoh Approach: Stingless bees mummify beetle parasites alive
Provedor de dados: 17
Autores: Mark K. Greco; Dorothee Hoffmann; Anne Dollin; Michael Duncan; Robert Spooner-Hart; Peter Neumann.
Social insect colonies usually live in nests, which are often invaded by parasitic species. Workers from these colonies use different
defence strategies to combat invaders. Nevertheless, some parasitic species are able to bypass primary colony defences due to
their morphology and behaviour. In particular, some beetle nest invaders cannot be killed or removed by workers of social bees,
thus creating the need for alternative social defence strategies to ensure colony survival. Here we show, using Diagnostic
Radioentomology, that stingless bee workers _Trigona carbonaria_, immediately mummify invading destructive nest parasites
_Aethina tumida_ alive, with a mixture of resin, wax and mud, thereby preventing severe damage to the colony. In sharp contrast
to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth & Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/2591/version/2
Bioconversion of eugenol into food flavouring agent vanillin
Provedor de dados: 17
Autores: Suaib Luqman; Sudeep Tandon; Alok Somvanshi; Suchita Srivastava; Mahendra P. Darokar; Suman P. S.
Khanuja.
Microorganisms have the ability to chemically modify a wide variety of organic compounds by a process referred to as biological
or microbial transformation, or in general, bioconversion. The microbial cells and their catalytic machinery (enzymes) accept a
wide array of complex molecules as substrates, yielding products with unparallel chiral (enantio-), positional (region-) and
chemical (chemo-) selectivity through various biochemical reactions. The present study was formulated on the objective of the
conversion of abundantly available phytomolecules eugenol into vanillin, a compound of industrial importance, using
microorganisms Aspergillus flavus, Aspergillus niger and Pseudomonas aeruginosa. These microbes were found to be capable of
converting eugenol...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6926/version/1
Genetic and Immune Predictors for Hypersensitivity Syndrome to Antiepileptic Drugs
Provedor de dados: 17
Autores: Manuela G. Neuman; Radu M. Nanau; Lawrence Cohen; Paul A. Hwang.
Hypersensitivity syndrome reactions (HSR) to antiepileptic drugs (AED) are associated with severe clinical cutaneous adverse
reactions (SCAR).Our aims are: to assess HSRs to AEDs using the in vitro lymphocyte toxicity assay (LTA) in patients who
manifested HSRs clinically, to correlate LTA results with the clinical syndrome, to correlate LTA results with the human
leukocyte antigen (HLA) allele B*1502 (HLA-B*1502) positivity in a Han Chinese-Canadian population, and to determine the
cytokine network in this population. HSR patients developed fever and cutaneous eruptions in the presence or absence of organ
involvement within 8 weeks of exposure to carbamazepine (CBZ), phenytoin (PHY) or lamotrigine (LTG). Control patients
received AEDs without presenting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6477/version/1
Simple reverse genetics approach to elucidating the biosynthetic pathway of complex thiopeptide
nocathiacin
Provedor de dados: 17
Autores: Maochen Wei; Jing Deng; Shuzhen Wang; Nan Liu; Yijun Chen.
Biothythetic pathway of the most drugable thiopeptide nocathiacin has been elucidated by applying reverse genetics method based
on its structural features. The present study provides an efficient approach for an easy access to the biosynthetic gene clusters of
complex bioactive peptides that are ribosomally synthesized with extensive posttranslational modifications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4740/version/1
Highly Efficient Gene Expression in B Lymphocytes Mediating by Lentivirus Vector
Provedor de dados: 17
Autores: Akira Tempaku.
Gene transduction and expression efficiencies among several type cell lines were compared by using vesicular stomatitis
virus-glycoprotein (VSV-G) pseudotyped human immunodeficiency virus type-1 (HIV-1) based lentivirus vector. Large
discrepancies of the efficiencies were shown among them. B lymphocytes showed high susceptibility of gene transduction and
expression, while other cell lines marked lower potential. Variable gene transduction strategies have been assessed to apply
immunological therapies. This study showed that B lymphocytes had facilities enough to support the gene transduction and
expression by lentivirus vector. Our result suggested that the lentivirus vector would be a powerful tool to express exogenous
genes in B lymphocytes.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7030/version/1
Improved prediction of protein interaction from microarray data using asymmetric correlation
Provedor de dados: 17
Autores: Kojiro Yano.
Background:Detection of correlated gene expression is a fundamental process in the characterization of gene functions using
microarray data. Commonly used methods such as the Pearson correlation can detect only a fraction of interactions between genes
or their products. However, the performance of correlation analysis can be significantly improved either by providing additional
biological information or by combining correlation with other techniques that can extract various mathematical or statistical
properties of gene expression from microarray data. In this article, I will test the performance of three correlation methods-the
Pearson correlation, the rank (Spearman) correlation, and the Mutual Information approach-in detection of protein-protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4430/version/1
Aptamer-based multiplexed proteomic technology for biomarker discovery
Provedor de dados: 17
Autores: Larry Gold; Deborah Ayers; Jennifer Bertino; Christopher Bock; Ashley Bock; Edward N. Brody; Jeff Carter; Virginia
Cunningham; Andrew Dalby; Bruce E. Eaton; Tim Fitzwater; Dylan Flather; Ashley Forbes; Trudi Foreman; Cate Fowler; Bharat
Gawande; Meredith Goss; Magda Gunn; Shashi Gupta; Dennis Halladay; Jim Heil; Joe Heilig; Brian Hicke; Gregory Husar;
Nebojsa Janjic; Thale Jarvis; Susan Jennings; Evaldas Katilius; Tracy R. Keeney; Nancy Kim; Terese Kaske; Tad Koch; Stephan
Kraemer; Luke Kroiss; Ngan Le; Daniel Levine; Wes Lindsey; Bridget Lollo; Wes Mayfield; Mike Mehan; Robert Mehler;
Michele Nelson; Sally K. Nelson; Dan Nieuwlandt; Malti Nikrad; Urs Ochsner; Rachel M. Ostroff; Matt Otis; Thomas Parker;
Steve Pietrasiewicz; Dan Resnicow; John Rohloff; Glenn Sanders; Sarah Sattin; Dan Schneider; Britta Singer; Martin Stanton;
Alana Sterkel; Alex Stewart; Suzanne Stratford; Jonathan D. Vaught; Mike Vrkljan; Jeffrey J. Walker; Mike Watrobka; Sheela
Waugh; Allison Weiss; Sheri Wilcox; Alexey Wolfson; Steve Wolk; Chi Zhang; Dom Zichi.
Interrogation of the human proteome in a highly multiplexed and efficient manner remains a coveted and challenging goal in
biology. We present a new aptamer-based proteomic technology for biomarker discovery capable of simultaneously measuring
thousands of proteins from small sample volumes (15 [mu]L of serum or plasma). Our current assay allows us to measure ~800
proteins with very low limits of detection (1 pM average), 7 logs of overall dynamic range, and 5% average coefficient of
variation. This technology is enabled by a new generation of aptamers that contain chemically modified nucleotides, which
greatly expand the physicochemical diversity of the large randomized nucleic acid libraries from which the aptamers are selected.
Proteins in complex...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4538/version/1
Screening of actinobacteria for antimicrobial activities by a modified
"Cross-Streak" method
Provedor de dados: 17
Autores: Nandkumar Kamat; Sonashia Velho-Pereira.
New molecule discovery from natural sources, such as that of actinobacteria, has proved to be an interesting area in antibiotic
research, as most of these antibiotics are difficult to synthesize. Out of 30 actinobacterial cultures screened for antimicrobial
activity, 28 cultures were found to produce active products against various pathogenic microorganisms such as Gram-negative,
Gram-positive bacteria and yeast, using a ‘modified cross streak method.' The modified method helped
in easy quantification of results and also in ruling out probable mutual antibiosis. 53%, 13% and 10% of tested actinobacterial
strains belonging to Streptomyces, Micromonospora and Actinomadura genera, respectively, showed the ability of producing
antimicrobial...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Microbiology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6765/version/1
Enhancing me - hope or hype?
Provedor de dados: 17
Autores: Pete Moore.
Many people talk about human enhancement as if it is about to happen. Some suggest that it already has. To join in the debate you
need to distinguish between social, personal and species enhancement. Social and personal are certianly with us - but talk of
species enhancement leans more to hype than hope.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2139/version/1
Advancing transcriptome platforms
Provedor de dados: 17
Autores: Shuobo Shi.
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination
of the transcriptome at unprecedented scale and speed. Studies using these methods have already altered our view of the extent
and complexity of transcript profiling, which has advanced from one-gene-at-a-time to a holistic view of the genome. Here, we
outline the major technical advances in transcriptome characterization, including the most popular used hybridization-based
platform, the well accepted tag-based sequencing platform, and the recently developed RNA-Seq (RNA sequencing) based
platform. Importantly, these next-generation technologies revolutionize assessing the entire transcriptome via the recent RNA-Seq
technology.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5894/version/1
Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene
Therapy Model
Provedor de dados: 17
Autores: C. S. Lin; Jhen Ru Shih; C. H. Hsu; Z.H. Wen; Chiranjib Chakraborty.
Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be
achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model. For this
purpose, we have chosen green fluorescent protein (GFP) as a target gene because the expression can be detected easily. GFP
was inserted in a plasmid vector, pQE30 to develop the vector pQE30GFP. The plasmid pQE30GFP was constructed form
plasmid, pQE30 and pEGFPC2. pQE30GFP injected directly in one group of fish into the muscle where luciferase expression was
noted. In another group, after injection electroporation was performed where we have also noted luciferase expression; but,
electroporation cause...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2718/version/1
Design and construction of a new Drosophila species, D.synthetica, by synthetic regulatory evolution
Provedor de dados: 17
Autores: Eduardo Moreno.
Here, I merge the principles of synthetic biology^1,2^ and regulatory evolution^3-11^ to create a new species^12-15^ with a
minimal set of known elements. Using preexisting transgenes and recessive mutations of Drosophila melanogaster, a transgenic
population arises with small eyes and a different venation pattern that fulfills the criteria of a new species according to
Mayr's "Biological Species Concept"^7,10^. The genetic circuit entails the loss of a
non-essential transcription factor and the introduction of cryptic enhancers. Subsequent activation of those enhancers causes
hybrid
lethality.
The
transition
from
"transgenic
organisms"
towards
"synthetic
species", such as Drosophila...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6511/version/1
Fractal-like Distributions over the Rational Numbers in High-throughput Biological and Clinical Data
Provedor de dados: 17
Autores: Vladimir Trifonov; Laura Pasqualucci; Riccardo Dalla-Favera; Raul Rabadan.
Recent developments in extracting and processing biological and clinical data are allowing quantitative approaches to studying
living systems. High-throughput sequencing, expression profiles, proteomics, and electronic health records are some examples of
such technologies. Extracting meaningful information from those technologies requires careful analysis of the large volumes of
data they produce. In this note, we present a set of distributions that commonly appear in the analysis of such data. These
distributions present some interesting features: they are discontinuous in the rational numbers, but continuous in the irrational
numbers, and possess a certain self-similar (fractal-like) structure. The first set of examples which we present here are drawn...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5037/version/1
A Universal Carrier Test for the Long Tail of Mendelian Disease
Provedor de dados: 17
Autores: Balaji S. Srinivasan; Jason Flannick; A. Scott Patterson; Christopher C. Chang; Tuan Pham; Sharon Young; Amit
Kaushal; James J. Lee; Pasquale Patrizio; Eric A. Evans.
*Background:* 

Mendelian disorders are individually rare but collectively common,
forming a "long tail" of genetic disease. More than 20 million people worldwide suffer from a disease in
this long tail before the age of 25, with minorities and developing countries at highest risk and with the number of carriers far in
excess of this figure. Importantly, the Jewish community’s campaign for universal Tay-Sachs screening shows that
these incurable diseases can nevertheless be prevented if carrier status is known before conception. A single highly-accurate assay
for the long tail of Mendelian disease would allow us to scale this successful campaign up to the general population, thereby
improving...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4192/version/1
The Modified Pharaoh Approach: Stingless bees mummify beetle parasites alive
Provedor de dados: 17
Autores: Mark K. Greco; Peter Neumann; Dorothee Hoffmann; Anne Dollin; Michael Duncan; Robert Spooner-Hart.
Social insect colonies usually live in nests, which are often invaded by parasitic species^1^. Workers from these colonies use
different defence strategies to combat invaders^1^. Nevertheless, some parasitic species are able to bypass primary colony
defences due to their morphology and behaviour^1-3^. In particular, some beetle nest invaders cannot be killed or removed by
workers of social bees^2-5^, thus creating the need for alternative social defence strategies to ensure colony survival. Here we
show, using Diagnostic Radioentomology^6^, that stingless bee workers _Trigona carbonaria_, immediately mummify invading
destructive nest parasites _Aethina tumida_ alive, with a mixture of resin, wax and mud, thereby preventing severe damage to the
colony. In...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth & Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/2591/version/1
Unlocking biomarker discovery: Large scale application of aptamer proteomic technology for early
detection of lung cancer
Provedor de dados: 17
Autores: Rachel M. Ostroff; William L. Bigbee; Wilbur Franklin; Larry Gold; Mike Mehan; York E. Miller; Harvey I. Pass;
William N. Rom; Jill M. Siegfried; Alex Stewart; Jeffrey J. Walker; Joel L. Weissfeld; Stephen Williams; Dom Zichi; Edward N.
Brody.
Lung cancer is the leading cause of cancer deaths, because ~84% of cases are diagnosed at an advanced stage. Worldwide in 2008,
~1.5 million people were diagnosed and ~1.3 million died – a survival rate unchanged since 1960. However, patients
diagnosed at an early stage and have surgery experience an 86% overall 5-year survival. New diagnostics are therefore needed to
identify lung cancer at this stage. Here we present the first large scale clinical use of aptamers to discover blood protein
biomarkers in disease with our breakthrough proteomic technology. This multi-center case-control study was conducted in
archived samples from 1,326 subjects from four independent studies of non-small cell lung cancer (NSCLC) in long-term
tobacco-exposed...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4537/version/1
QSAR and Docking Studies of Gallic Acid Derivatives for Anticancer and Immunomodulatory
Activity
Provedor de dados: 17
Autores: Dharmendra Kumar Yadav; Feroz Khan; Arvind Singh Negi.
Immuno-modulation is the process of alteration in immune response due to foreign intrusion of molecules inside the body. In the
present communication QSAR and molecular docking studies for immuno-modulatory activity of derivatives of gallic acid were
performed. Molecular docking studies showed higher binding affinity with INFα-2, IL-6, and IL-4 receptors. Binding
site residues indicate hydrogen bonds formation with gallic acid derivative compounds G-3, G-4, G-5, G-6, G-7 and G-10. Further
screening through quantitative structure activity relationship model establishes the immuno-modulatory activity of the compounds
G-4, G-7, G-9, G-10, G-12 and G-13. Activity was predicted through a robust statistical model, developed by forward feed
multiple...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6620/version/1
High-Value Phytochemicals from Grape Cane Waste: Potential Value-Added Viticultural Sources of
Trans-Resveratrol and Trans-e-Viniferin with Medicinal and Anti-Phytopathogenic Applications
Provedor de dados: 17
Autores: Sierra Rayne.
Grape canes as agricultural waste from commercial viticultural activities represent a potentially important source of the
well-known medicinal and anti-phytopathogenic stilbene compounds trans-resveratrol and trans-e-viniferin. Reports in the
literature suggest that concentrations of these compounds range up to 5 mg/g dw and 2 mg/g dw, respectively, and can be
quantitatively extracted from the cane residue using low-cost, environmental benign, and non-toxic aqueous alcoholic solvent
systems such as ethanol:water mixtures. With current commercial values of these compounds between US$2,000 to US$3,000
per kg, established stilbene yields from cane waste could represent an agricultural coproduct valued at US$2,000 to US$3,000 per
hectare of production. At the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Earth & Environment.
Ano: 2007
URL: http://precedings.nature.com/documents/636/version/1
Structure and function prediction of human homologue hABH5 of _E. coli_ ALKB5 using in silico
approach
Provedor de dados: 17
Autores: shankaracharya shankaracharya; Saibal Das; Ambarish Sharan Vidyarthi.
Newly discovered human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer
therapy. Little is known about the structure and function of hABH5, one of the members of this superfamily. Therefore, in the
present study we intend to predict its structure and function using various bioinformatics tools. Modeling was done with modeler
9v7 to predict the 3D structure of the hABH5 protein. 3-D model of hABH5, ALKBH5.B99990005.pdb was predicted and
evaluated. Validation results showed 96.8% residues in favor and an additional allowed region of the Ramachandran plot. Ligand
binding residues prediction showed four ligand clusters, having 25 ligands in cluster 1. Importantly, conserved pattern of
Pro158-X-Asp160-Xn-His266 in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5597/version/1
Pepper yellow leaf curl Indonesia virus, a new bipartite begomovirus species that belongs to distinct
clade of Old World geminiviruses
Provedor de dados: 17
Autores: Pradeep Sharma1,2; J. Sakata2; Y. Shibuya2; Masato Ikegami2.
Begomoviruses are currently emerging as a major threat to vegetable production in many tropical and subtropical regions
worldwide. Pepper yellow leaf curl disease (PepYLCD) has been noticed in many Capsicum annum L. producing regions from
East Asia especially from Indonesia and causes devastating damage to pepper crop production since 2000. In this study we have
cloned and sequenced complete nucleotide of begomoviruses from pepper exhibiting leaf curling and bright yellowing symptoms.
Besides, we also determined the occurrence of disease on tomato evoking leaf curl symptoms and ageratum with yellow vein type
of symptoms. On the basis of genome organization and sequence homology, these viruses were designated as Pepper yellow leaf
curl Indonesia virus...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4162/version/1
Chickpea Genomics Consortium on Drought Tolerance
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genomes; Chickpeas; Biotechnology; Genes; Tolerance; Weather hazards.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3324
Tissue culture parameters in sweet orange cultivars
Provedor de dados: 86
Autores: Mendes,Amanda Ferreira da Silva; Cidade,Luciana Cardoso; Manzoli,Gabrielle Novais; Otoni,Wagner Campos; Soares
Filho,Walter dos Santos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants
with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with
alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot
development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant
in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was
significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Journal article
Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020
Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
Provedor de dados: 86
Autores: Silva,Thiago Édson Ribeiro da; Cidade,Luciana Cardoso; Alvim,Fátima Cerqueira; Cascardo,Júlio Cézar de Mattos;
Costa,Marcio Gilberto Cardoso.
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao
L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli
growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium.
Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo
induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes,
irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
Tipo: Journal article
Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000024
Microbial diversity, metagenomics and the Yucatán aquifer
Provedor de dados: 85
Autores: Rojas-Herrera,R; Zamudio-Maya,M; Arena-Ortiz,L; Pless,RC; O'Connor-Sánchez,A.
Mexico counts among the five countries with the highest biodiversity in the world. In the Yucatán Peninsula, there are aquatic
ecosystems with a very special microbial diversity. These ecosystems are essential for the ecological equilibrium of the region,
and are seriously threatened by human activities. Access and knowledge of the microbial resources of these environments have an
enormous scientific interest, and could potentially result in biotechnological products which could lead to more efficient and
environmentally friendly processes; it could also offer a full arsenal of microorganisms and/or novel molecules to the local and
world industry to face the current needs. In recent years, with the rise of metagenomic technology, knowledge about microbial...
Tipo: Journal article
Palavras-chave: Ecosystems; Biotechnology; 454-sequencing.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000200017
Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and
'Poncã' mandarin
Provedor de dados: 94
Autores: Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene
Alves de.
This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated
charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and
'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to
the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of
sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of
sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1).
After the...
Tipo: Journal article
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031
Scientific progress in the production of monogastric in the first decade of the twenty-first century
Provedor de dados: 96
Autores: Costa,Fernando Guilherme Perazzo; Silva,José Humberto Vilar da; Lima,Raffaella Castro de; Oliveira,Cleber Franklin
Santos de; Rodrigues,Valéria Pereira; Pinheiro,Sarah Gomes.
Significant progress in the area of animal production is due to the development of researches on genetics, environment, health,
management and nutrition. The search for better management conditions that benefit animal welfare resulted in changes to the
installation. The genetic improvement more productive animals has encouraged the number of searches to reduce the incidence of
diseases, mainly of metabolic origin. The incorporation in products with higher bioavailability as industrial amino acid and
organic minerals, the inclusion of products modifiers enteric of microflora as probiotic, prebiotic, symbiotic, nucleotides and
exogenous enzymes, has benefited the use of nutrients are not well absorbed and often unavailable in the diet. Biotechnology
latest...
Tipo: Journal article
Palavras-chave: Amino acids; Biotechnology; Nutrition; Phenomenal progress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300032
Cattle farmers: profile and speech content analysis while undergoing training to adopt artificial
insemination in Goiás State, Brazil
Provedor de dados: 96
Autores: Gordo,João Maurício Lucas; Silva,Marcelo Corrêa da; Solano,Gianice de Almeida; Lopes,Fernando Brito;
Costa,Marcos Fernando Oliveira e; Rocha,Francisco Eduardo de Castro; Fioravanti,Maria Clorinda Soares; Sereno,José Robson
Bezerra.
This study was undertaken to verify major issues present in the discourse of cattle farmers when discussing the use of artificial
insemination (AI). Seventy-one beef and dairy cattle farmers in the state of Goiás, Brazil, were interviewed using semi-structured
questionnaires during AI training courses in 2010 and 2011. Data analyses were done using the classic method of textual speech
content analysis, performing the pre-analytical, analytical and inferential stages. The written questions were separated in
elementary context units (ECU) and sorted into similar thematic groups. These groups originated categories and subcategories,
which were nominated based on an a posteriori criteria. Overall, personal motivation was the main issue characterizing
discourse...
Tipo: Journal article
Palavras-chave: Animal reproduction; Biotechnology; Discourse analysis; Social psychology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000300003
Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
Provedor de dados: 63
Autores: Monteiro,Mariza; Appezzato-da-Glória,Beatriz; Valarini,Maria José; Oliveira,Carlos Alberto de; Vieira,Maria Lucia
Carneiro.
Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this
study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic
embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a
protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended
for protoplast electroporation-mediated genetic transformation of alfalfa.
Tipo: Journal article
Palavras-chave: Plant biotechnology; In vitro culture; Morphogenesis; Forage legume.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
Haplodiploid androgenetic breeding in oat: genotypic variation in anther size and microspore
development stage
Provedor de dados: 63
Autores: Cesaro,Taniela De; Baggio,Maria Irene; Zanetti,Silvia Andréia; Suzin,Marilei; Augustin,Lizete; Brammer,Sandra
Patussi; Iorczeski,Edson Jair; Milach,Sandra Cristina Kothe.
Oat (Avena spp.) is poorly responsive to the haplodiploidization process, which leads to the production of homozygous lines in
one step, increasing breeding efficiency. Androgenetic haploids in small grain cereal crops are obtained from microspores cultured
at the mononucleate stage, which can be identified by the size of anthers. In order to identify the appropriate anther size for in
vitro culture, microspore cytological analyses were made in Avena sativa cultivars UPF 7, UPF 18, UFRGS 14, Stout and Avena
sterilis CAV 3361, cultivated in growth chamber under controlled light and temperature conditions. Variation was observed within
and among genotypes for anther size at each microspore developmental stage and according to the position of spikelets in the...
Tipo: Journal article
Palavras-chave: Androgenesis; Anther culture; Biotechnology; Haploid breeding; Microspores.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000100016
Patentes em biotecnologia: estímulo ou obstáculo à produção de conhecimento e inovações?
Provedor de dados: 119
Autores: SCHNEIDER, J. E.
1993
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Patente; Biotecnologia; Inovacao; Propriedade intelectual; Biotechnology; Innovation; Patents; Intellectual
property.
Ano: 1993
URL: http://www.alice.cnptia.embrapa.br/handle/doc/95248
Análise de RAPD em calos, plantas regeneradas e "seedlings" de milho (Zea mays L.).
Provedor de dados: 119
Autores: VASCONCELOS, M. J. V.; CARVALHO, C.H.S. de; PAIVA, E.; GUIMARAES, C.T.; MACHADO, M.A.; BARROS,
E.G.
1993
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; RAPD; Zea mays; Maize; Biotechnology; Seedlings.
Ano: 1993
Importância econômica e bioecologia da broca Elasmopalpus lignosellus.
Provedor de dados: 119
Autores: VIANA, P. A.
1993
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Milho; Pragas; Bioecologia; Elasmopalpus lignosellus; Zea mays; Maize; Pest; Biotechnology.
Ano: 1993
Cattle farmers: profile and speech content analysis while undergoing training to adopt artificial
insemination in Goiás State, Brazil.
Provedor de dados: 119
Autores: GORDO, J. M. L.; SILVA, M. C. da; SOLANO, G. de A.; LOPES, F. B.; COSTA, M. F. O. e; ROCHA, F. E. de C.;
FIORAVANTI, M. C. S.; SERENO, J. R. B.
2013
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Reprodução animal; Biotecnologia; Inseminação artificial; Biotechnology; Artificial insemination; Animal
reproduction.
Ano: 2013
Anais: resumos dos trabalhos.
Provedor de dados: 119
Autores: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 5.,
2000, Brasilia, DF.
2000
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Controle biologico; Biotecnologia; Recurso genetico; Biological control; Biotechnology; Genetic resources.
Ano: 2000
Biotecnologia no melhoramento do milho.
Provedor de dados: 119
Autores: PAIVA, E.
1990
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Milho; Melhoramento; Biotecnologia; Zea mays; Maize; Breeding; Biotechnology.
Ano: 1990
Anais: resumos dos trabalhos.
Provedor de dados: 119
Autores: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7.,
2002, Brasília, DF.
2002
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Recursos geneticos; Biotecnologia; Controle biologico; Genetic resources; Biotechnology; Biological control.
Ano: 2002
URL: http://www.alice.cnptia.embrapa.br/handle/doc/184607
Estudo da evolução das biotécnicas de transferência de embriões e fertilização in vitro na raça Nelore
no Brasil.
Provedor de dados: 119
Autores: BELTRAME, R. T.; QUIRINO, C. R.; BARIONI, L. G.
Arquivos da Associação Brasileira dos Criadores de Zebu foram utilizados para traçar um panorama da evolução das biotécnicas
de transferência de embriões e fertilização in vitro dos anos de 1997 a 2007 para a raça Nelore no Brasil. Registros de 109.775
coletas de embriões e de 73.121 aspirações foram disponibilizadas para análise. Observou-se a supremacia da técnica de
transferência de embriões até o ano de 2004 com posterior crescimento da fertilização in vitro. As regiões Sudeste e Centro Oeste
se destacaram na execução de ambas as biotécnicas. As médias de embriões viáveis produzidos pelas duas biotécnicas no decorrer
dos anos nas regiões do país se encontram próximas as relatadas pela literatura (entre cinco e sete embriões por coleta ou
aspiração).
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Banco de dados; Biotecnologia; Transferência de embriões; Fertilização in vitro; Databases; Biotechnology;
Embryo.
Ano: 2010
URL: http://www.alice.cnptia.embrapa.br/handle/doc/875489
Anais: resumos dos trabalhos.
Provedor de dados: 119
Autores: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8.,
2003, Brasília, DF.
2003
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Recursos geneticos; Biotecnologia; Controle biologico; Genetic resources; Biotechnology; Biological control.
Ano: 2003
URL: http://www.alice.cnptia.embrapa.br/handle/doc/174686
Anais: resumos dos trabalhos.
Provedor de dados: 119
Autores: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4.,
1999, Brasília, DF.
1999
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Recursos geneticos; Controle biologico; Biotecnologia; Biological control; Biotechnology; Genetic resources.
Ano: 1999
URL: http://www.alice.cnptia.embrapa.br/handle/doc/179268
Caracterização molecular e bioquímica de populações de milho indígena (Zea mays L.).
Provedor de dados: 119
Autores: GUIMARAES, C.T.; VASCONCELOS, M. J. V.; BARROS, E.G.; PAIVA, E.
1994
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; Biologia Molecular; Zea mays; Maize; Biotechnology; Molecular biology.
Ano: 1994
Biotecnologia aplicada no Nordeste brasileiro: reflexoes sobre as hortalicas.
Provedor de dados: 119
Autores: FARI, M.
1999
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Biotecnologia; Hortalica; Brasil; Nordeste; Biotechnology; Vegetables; Brazil; Northeast.
Ano: 1999
URL: http://www.alice.cnptia.embrapa.br/handle/doc/131974
Detecção de polimorfismo genético em milho através da amplificação do DNA pela reação em cadeia
da polimerase (RAPD).
Provedor de dados: 119
Autores: CARVALHO, C.C. de; ANDREOLI, C.; PAIVA, E.
1993
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; RAPD; Polimorfismo; Zea mays; Maize; Biotechnology; Genetic polymorphism.
Ano: 1993
Effect of ABA and GA3 during germination of Anadenanthera peregrina (L.) speg. embryos.
Provedor de dados: 119
Autores: PAIVA, R.; BARDUCHE, D.; LOPES, M.A.; PAIVA, E.
1996
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Germinacao; Biotecnologia; Biologia molecular; Germination; Biotechnology; Molecular biology.
Ano: 1996
Anais: resumos dos trabalhos.
Provedor de dados: 119
Autores: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6.,
2001, Brasília, DF.
2001
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Recursos geneticos; Biotecnologia; Controle biologico; Genetic resources; Biotechnology; Biological control.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/handle/doc/180288
Biochemical markers for nitrogen use efficience in maize.
Provedor de dados: 119
Autores: PURCINO, A. A. C.; SILVA, M.R.e; PAIVA, E.; ANDRADE, S.R.M.
1993
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Nitrogenio; Biotecnologia; Marcadores bioquimia; Zea mays; Maize; Nitrogen; Biotechnology.
Ano: 1993
Desenvolvimento e contribuições da biotecnologia no melhoramento genético do milho.
Provedor de dados: 119
Autores: CARNEIRO, N. P.; PAIVA, E.
2000
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; Melhoramento; Zea mays; Maize; Breeding; Biotechnology.
Ano: 2000
Web semântica na Embrapa: o próximo desafio da gestão do conhecimento.
Provedor de dados: 119
Autores: SOUZA, K. X. S. de; MASSRUHÁ, S. M. F. S.; KUSER-FALCÃO, P.
Um problema que enfrentamos hoje na Web é que junto com a informação que buscamos, retorna uma quantidade de informações
irrelevantes. Projetada pelos idealizadores da Web, a Web Semântica propõe-se a estruturar a rede de forma que seus dados
possam ser compreendidos por qualquer computador nela conectado, refinando essa busca e filtrando do conhecimento que nos
interessa, aliviando a sobrecarga de informação com o aumento da precisão.
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Recuperação da informação; Web; Biotecnologia; Web semântica; Ontologia; Information retrieval;
Biotechnology.
Ano: 2008
URL: http://www.alice.cnptia.embrapa.br/handle/doc/31594
Genômica e biotecnologia aplicadas a adaptação a mudanças climáticas.
Provedor de dados: 119
Autores: GERHARDT, I. R.; DANTE, R. A.
Mudanças climáticas globais e seus impactos na agricultura. Pesquisa e desenvolvimento de tecnologias transgênicas. Genômica
funcional para descoberta de genes de valor agronômico. Bancos de dados genômicos. Genômica comparativa. Análise
filogenética de famílias gênicas. Aplicações de tecnologias de sequenciamento de próxima geração. Seleção em larga escala de
eventos transgênicos. A Unidade Mista de Pesquisa em Genômica Aplicada a Mudanças Climáticas (UMiP GenClima).
Tipo: Capítulo em livro técnico-científico (ALICE)
Palavras-chave: Mudanças climáticas; Genômica; Biotecnologia; Climate change; Genomics; Biotechnology.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010699
Differentiation of colletrotrichum truncatum isolates by random amplified polymorphic DNA.
Provedor de dados: 119
Autores: VASCONCELOS, M. J. V. de; MACHADO, M.A.; ALMEIDA, A.M.R.; HENNING, A.A.; BARROS, E.G.de;
MOREIRA, M.A.
Cinco isolados de Colletotrichum truncatum foram obtidos de hastes infectadas de soja (4) e crotalaria (1), coletadas em Londrina
(PR), Ponta Grossa (PR) e Candido Mota (SP). Apos isolamento de desenvolvimento em meio liquido (batata-dextrose), o micelio
foi utilizado para extracao do DNA. Amostras de DNA de cada isolado foram amplificadas utilizando-se "primers" de sequencias
arbitrarias, pela tecnica de amplificacao ao acaso de DNA polimorfico (RAPD). Geis de agarose, apos separacao eletroforetica dos
produtos de amplificacao, mostraram bandas polimorficas que permitiram avaliar a dissimilaridade genetica entre os cinco
isolados. Estes podem ser agrupados em tres grupos distintos de acordo com os perfis apresentados. Os isolados GL-614 e Preto...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Sorgo; Doencas; Biotecnologia; Sorghum bicolor; Colletotrichum truncatum; Biotechnology.
Ano: 1994
In vitro organogenesis from internodal segments of adult sweet orange plants.
Provedor de dados: 119
Autores: BASSAN, M. M.; MOURÃO FILHO, F. de A. A.; MIYATA, L. Y.; MENDES, B. M. J.
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and
'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of
6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour
photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and
Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Citrus sinensis; Adventitious buds; Biotechnology; Plant growth regulators.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/handle/doc/901813
Genetic dissection of maturity using RFLPs.
Provedor de dados: 119
Autores: PHILLIPS, R.L.; KIM, T.S.; KAEPPLER, S.M.; PARENTONI, S. N.; SHAVER, L.; STUCKER, R.E.; OPENSHAW,
S.J.
Several genetic regions having major effects on maturity have been identified using RFLP analysis. One such region on
chromosomes 8 is important across several diverse genotypes and ccounts for up to 50% of the variation for maturity in a cross
involving an inbred line (N28) and a 20-backcross generation derivative (N28E). In addition to the chromsome 8 QTL for
maturity, we have evidence using the backcross-derived line approach for regions controlling maturity on chromosomes 1, 2, 3, 5,
7, and 9. Chromosome 5 appears to be especially important in both magnitude of effect and across several genetic hackground.
Maturity as measured by days to pollen shed or silking may be controlled by additive or nearly dominant gene action depending
on the chromosome...
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; RFLP; Melhoramento; Zea mays; Maize; Biotechnology; Breeding.
Ano: 1992
Genetic modification of mammalian genome at chromosome level
Provedor de dados: 42
Autores: SEROV,OLEG L.; MATVEEVA,NATALIA M.; SEROVA,IRINA A.; BORODIN,PAVEL M.
The review is concerned with a progress in genetic modification of a mammalian genome in vitro and in vivo at chromosomal
level. Recently three new approaches for the chromosome biotechnology have been developed: Using Cre/loxP-system a
researcher is able to produce targeted rearrangements of whole chromosomes or their segments or particular genes within the
genome, and therefore to modify the set, position and copy number of the endogenous elements of the genome. Mammalian
artificial chromosomes (MACs) provide a possibility to introduce into genome relatively large segments of alien chromosome
material, either artificially constructed or derived from the genome of different species. Using ES-somatic cell hybrids allows to
transfer whole chromosomes or...
Tipo: Journal article
Palavras-chave: Chromosome biotechnology; Cre/loxP; ES cells; MAC.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300012
TAPCells, the Chilean dendritic cell vaccine against melanoma and prostate cancer
Provedor de dados: 48
Autores: Salazar-Onfray,Flavio; Pereda,Cristián; Reyes,Diego; López,Mercedes N.
Here we summarize 10 years of effort in the development of a biomedical innovation with global projections. This innovation
consists of a novel method for the production of therapeutic dendritic-like cells called Tumor Antigen Presenting Cells
(TAPCells®). TAPCells-based immunotherapy was tested in more than 120 stage III and IV melanoma patients and 20
castration-resistant prostate cancer patients in a series of phase I and I/II clinical trials. TAPCells vaccines induced T
cell-mediated memory immune responses that correlated with increased survival in melanoma patients and prolonged
prostate-specific antigen doubling time in prostate cancer patients. Importantly, more than 60% of tested patients showed a
Delayed Type Hypersensitivity (DTH) reaction...
Tipo: Journal article
Palavras-chave: Dendritic Cells; Cancer Immunotherapy; Melanoma Vaccine; Technology Transfer; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400014
Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude
extract
Provedor de dados: 58
Autores: Duarte,Lívia Teixeira; Tiba,Joyce Batista; Santiago,Mariângela Fontes; Garcia,Telma Alves; Bara,Maria Teresa Freitas.
Tyrosinase is an enzyme of industrial interest. The production and characterization of tyrosinase from P. sanguineus CCT-4518
were investigated. The selection of inductors, luminosity influence, inoculum size and type of culture medium on the production
of tyrosinase and the effect of inhibitors on enzyme activity were performed. Optimum conditions for intracellular tyrosinase
production was observed after 2 days using 0.15% L-tyrosine as inducer, in the presence of light, with inoculum size of 10
mycelium discs, using 2% malt extract broth medium, incubated at 30°C, and constant agitation of 150 rpm. Tyrosinase activity
was completely inhibited by the addition of 6 mM salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was
recorded by...
Tipo: Journal article
Palavras-chave: Pycnoporus sanguineus; White-rot fungi; Tyrosinase; MBTH; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100003
Isolation and screening of d-limonene-resistant microorganisms
Provedor de dados: 58
Autores: Bicas,Juliano Lemos; Pastore,Gláucia Maria.
This study reports the isolation of microorganisms that are resistant to environment containing limonene, the most important
residue in the citrus industry. For the isolation, samples collected from strategic places of a citrus processing plant (yellow water,
entrance and exit of the bagasse tank, effluent and deteriorated fruits found in bins, machine straps, fruit washers and plant floor),
some citrus fruit and mint from local market were used. The samples were incubated in rotary shaker at 30ºC/150rpm for 48h or 7
days in YM medium containing 0.1% limonene. Great part of the 112 strains recovered after 48h and the 126 strains recovered
after 7 days were identified as Gram positive bacilli, followed by Gram negative bacilli, yeasts and Gram positive...
Tipo: Journal article
Palavras-chave: Limonene; Industrial by-products; Flavor; Biotechnology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300034
Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts
from Alternanthera maritima (Amaranthaceae)
Provedor de dados: 58
Autores: Salvador,Marcos J.; Pereira,Paulo S.; França,Suzelei C.; Candido,Regina C.; Ito,Izabel Y.; Dias,Diones A.
The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal
combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating
the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the
agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts
and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants
collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of
several...
Tipo: Journal article
Palavras-chave: Alternanthera maritima; Adult plants; Callus culture; Biotechnology; Antimicrobial activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100022
Drugs obtained by biotechnology processing
Provedor de dados: 60
Autores: Almeida,Hugo; Amaral,Maria Helena; Lobão,Paulo.
In recent years, the number of drugs of biotechnological origin available for many different diseases has increased exponentially,
including different types of cancer, diabetes mellitus, infectious diseases (e.g. AIDS Virus / HIV) as well as cardiovascular,
neurological, respiratory, and autoimmune diseases, among others. The pharmaceutical industry has used different technologies to
obtain new and promising active ingredients, as exemplified by the fermentation technique, recombinant DNA technique and the
hybridoma technique. The expiry of the patents of the first drugs of biotechnological origin and the consequent emergence of
biosimilar products, have posed various questions to health authorities worldwide regarding the definition, framework, and...
Tipo: Journal article
Palavras-chave: Biotechnology; Recombinant DNA; Hybridoma technique; Bacteria; Antibiotics; Vaccines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200002
Managing agricultural biotechnology in Colombia
Provedor de dados: 69
Autores: Schuler,Ingrid; Orozco,Luis Antonio.
The international scenario for biotechnology shows a rapid tendency at industrialized countries in the increase of publications,
patents, enterprises and novel solutions for the industry, the environment, health and agriculture. Nevertheless, Colombia has an
important delay in relation to the international scientific development and the capacity to generate wealth and services for its
productive systems. This delay has been one of the concerns of the country's policy during the last years, and more precisely since
2002, when for the first time biotechnology was included in a National Development Plan as one of the mechanisms for
competitiveness and the use of biodiversity and genetic resources. This paper is the result of a survey conducted in 2005 aimed...
Tipo: Journal article
Palavras-chave: Agricultural biotechnology; Indicators; Legislation; Policies; Public perception.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300001
Genetic engineering applications in animal breeding
Provedor de dados: 69
Autores: Montaldo,Hugo H.
This paper discusses the use of genetic engineering applications in animal breeding, including a description of the methods, their
potential and current uses and ethical issues. Genetic engineering is the name of a group of techniques used to identify, replicate,
modify and transfer the genetic material of cells, tissues or complete organisms. Important applications of genetic engineering in
animal breeding are: 1) Marker-assisted selection (MAS). The objective of this technology is to increase disease resistance,
productivity and product quality in economically important animals by adding information of DNA markers to phenotypes and
genealogies for selection decisions. 2) Transgenesis, the direct transfer of specific genes/alleles between individuals,...
Tipo: Journal article
Palavras-chave: Adult mammalian cloning; Biotechnology; Gene mapping; GMOS; MAS; QTL; Transgenics.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200010
Natural antifouling compound production by microbes associated with marine macroorganisms: A
review
Provedor de dados: 69
Autores: Satheesh,Sathianeson; Ba-akdah,Mohammad Abdulaziz; Al-Sofyani,Abdulmohsin A.
In the marine environment, all hard surfaces including marine macroorganims are colonized by microorganisms mainly from the
surrounding environment. The microorganisms associated with marine macroorganisms offer tremendous potential for
exploitation of bioactive metabolites. Biofouling is a continuous problem in marine sectors which needs huge economy for control
and cleaning processes. Biotechnological way for searching natural product antifouling compounds gained momentum in recent
years because of the environmental pollution associated with the use of toxic chemicals to control biofouling. While, natural
product based antifoulants from marine organisms particularly sponges and corals attained significance due to their activities in
field assays,...
Tipo: Journal article
Palavras-chave: Antifouling; Biofouling; Bioactive compounds; Marine biotechnology; Marine microbes.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300012
Food biotechnology and education
Provedor de dados: 69
Autores: Ramón,Daniel; Diamante,Alicia; Calvo,María Dolores.
It is clear that the future of our children will be marked by the development of two scientific disciplines: computing and
biotechnology. Regarding the first, during recent years considerable progress have been done in many countries around the globe
aimed at enhancing the teaching in these subjects and, at the same time, encouraging the use of computers in classrooms. On the
contrary, training in biotechnology is absent in many secondary schools of the planet. This formative deficiency generates citizens
whose opinion on the marketing of biotechnological products is easily manipulated by both defenders and opposers of
biotechnology. This situation is of particular relevance when the item in question is food biotechnological applications that
provoke an...
Tipo: Journal article
Palavras-chave: Food biotechnology; Genetically modified foods; Public perception; Young consumers.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500001
The relationship among knowledge of, attitudes toward and acceptance of genetically modified
organisms (GMOs) among Slovenian teachers
Provedor de dados: 69
Autores: Sorgo,Andrej; Ambrozis-Dolinsek,Jana.
The objective of this study was to investigate knowledge about, opinions on and attitudes toward and finally readiness to accept
genetically modified organisms (GMO) among Slovenian teachers. On average, they have higher levels of knowledge in classical
genetics, and poor levels of knowledge about modern issues in biotechnology, and their attitudes toward GMOs are not extreme.
They make decisions based on the acceptability of a particular GMO and not on GMOs in general, following two patterns: genetic
modifications (GM) microorganisms and plants are more acceptable than animals, and GMOs are more acceptable if they can not
be used directly for consumption and produce something recognized as useful. The relationship among knowledge of, attitudes
towards and...
Tipo: Journal article
Palavras-chave: Acceptance; Attitudes; Biotechnology; Education; Genetically modified organisms; Knowledge.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400001
Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings
subjected to low temperature conditions
Provedor de dados: 69
Autores: Rasmussen-Poblete,Susana; Valdes,Jorge; Gamboa,Maria Cecilia; Valenzuela,Pablo D.T; Krauskopf,Erwin.
Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to
its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly
available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced
9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons
forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant
similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public
resource that...
Tipo: Journal article
Palavras-chave: Cellulose; Cold-temperature; EST database; Forest biotechnology; Lignin.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008
Comportamento e descrição da linhagem transgênica BRASD 00-12057 RR no estado de Goiás e no
Distrito Federal (folder).
Provedor de dados: 14
Autores: FARIAS NETO, A. L. de; MOREIRA, C. T.; SOUZA, P. I. M. de; ABUD, S.; ALMEIDA, L. A. de; TOLEDO, J. F. F.;
NUNES JÚNIOR, J.; MONTEIRO, P. M. F. O.; DI STEFANO, J. G.; ASSUNÇÃO, M. S.; ARANTES, N. E.; YORINORI, J. T.;
TEIXEIRA, R. N.; DIAS, W. P.; ALMEIDA, A. M. S.; TOLEDO, R. M. C. P.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Soja; Glycine max; Transgênico; Biotecnologia; Soybeans; Transgenics; Biotechnology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570203
Genética e biotecnologia aplicada ao melhoramento genético vegetal: relatório de pós-doutorado.
Provedor de dados: 14
Autores: FALEIRO, F. G.
2012
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Genética; Melhoramento vegetal; Biotecnologia; Biotechnology; Genetics; Plant breeding.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/958925
Resumo dos trabalhos...
Provedor de dados: 14
Autores: TALENTO ESTUDANTIL DO CENARGEN, 1996, Brasília, DF.
A evolução dos mapas genéticos; Influência da temperatura de cultivo no crescimento e toxicidade do isolado S93 de Bacillus
thuringiensis supesp. Kurstaki; Efeito da previsibilidade alimentar no vigor da prole de Podisum nigrispinus; Diagnóstico
molecular para uso e preservação de germoplasma equino.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biologia; Controle biológico; Genética; Biotecnologia; Biology; Genetic; Biotechnology.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/171474
Sistema de Informação da Rede Genômica Animal - Sirga: manual do usuário.
Provedor de dados: 14
Autores: HIGA, R. H.; LONG, C. K.
O software Sirga baseia-se em uma plataforma de banco de dados relacional e acesso via web, tal que o sistema pode ser acessado
de qualquer local que tenha acesso à internet. Este documento apresenta os pré-requisitos necessários para utilização do sistema e
uma descrição de suas funcionalidades.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software Sirga; Biotecnologia; Microarranjos; Computer software; Microarray technology; Biotechnology.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/920174
O avanço da tecnologia OGM no mercado do milho no Brasil.
Provedor de dados: 14
Autores: GARCIA, J. C.; DUARTE, J. de O.
2011
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Biotecnologia; Alimento transgênico; Zea mays; Biotechnology; Transgenics; Transgenic foods.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/897712
Cultivares de soja transgênicas da Embrapa indicadas para o sistema de produção da região do
cerrado (folder).
Provedor de dados: 14
Autores: SOUZA, P. I. M. de; MOREIRA, C. T.; FARIAS NETO, A. L.; ABUD, S.; NUNES JÚNIOR, J.; MONTEIRO, P. M. F.
O.; DIAS, W. P.; ALMEIDA, L. A.; TOLEDO, J. F. F.; ASSUNÇÃO, M. S.; DI STEFANO, J. G.; ARANTES, N. E.; TOLEDO,
R. M. C. P.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Soja; Glycine max; Transgênico; Biotecnologia; Soybeans; Transgenics; Biotechnology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570161
Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and
Degradation
Provedor de dados: 5
Autores: Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire;
Weiss, Pierre; Colliec-jouault, Sylvia.
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause
considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of
the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously
demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method,
other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess
engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas)
was compared...
Tipo: Text
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight
distribution; Rheology.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf
Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp
MC3B-10
Provedor de dados: 5
Autores: Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne;
Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component,
molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of
temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic
molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan
may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest
emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached
its...
Tipo: Text
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf
Flexibility in Cucurbitaceae breeding management with an integrated research software
Provedor de dados: 35
Autores: Sillon, J.-F.; Royer, F.; Royer, Fl.
DORIANE SAS develops and commercializes integrated and modular software for research activity able to manage the breeding
data with a high level of flexibility. This software is able to manage complex “experiment cycles” and molecular markers data
required by Cucurbitaceae breeding. Thanks to two innovative concepts like "experiment cycle" and "multi level architecture",
DORIANE reached this objective building a well organized library adapted for the whole research department and a powerful tool
for assisted decision making. Moreover, its client-server architecture facilitates the transmission of information between
researchers and laboratories.
Tipo: Poster
Palavras-chave: Cucurbitaceae; Biotechnology; Breeding; Database; Experiments management; Molecular marker.
Ano: 2008
URL: http://hdl.handle.net/2174/253
Determination of Isoflavone Contents for Selected Soybean Lines and Soy Foods by Fourier
Transform Near Infrared Reflectance Spectroscopy
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Randall L. Nelson.
Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets. Rapid and economical
determination of soybean isoflavone contents is essential for breeding and selection of soybean seeds with optimal isoflavone
levels for food applications. Fourier Transform Near Infrared Spectroscopy (FT-NIR) calibrations were developed for rapid and
reliable analysis of soybean isoflavone content. Our isoflavone calibrations are characterized by low standard errors
(<0.02%) and high degrees of correlation (>99%).
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Immunology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6194/version/1
Structural and functional validation of Microsystin synthetases
Provedor de dados: 17
Autores: Swapnil G. Sanmukh; Waman N. Paunikar.
The structure and function prediction for the Microsystin synthetases from Microsystis aerogenosa (LNSAMB) were carried out
for verifying the authenticity of the sequenced genes. The genes for Microsystin synthetases (mcyA, mcyB, mcyD and mcyE,),
were predicted by the application of computational methods and Bioinformatics web tools. The probable function prediction for
the proteins was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching
protein databases for the presence of conserved domains. While tertiary structures were constructed using PS2 Server- Protein
Structure Prediction server. This study revealed structural and functional validation of sequenced genes.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6835/version/1
Signatures of spatial organizations – From the universe of proteins to the universe in
general
Provedor de dados: 17
Autores: Aditya Mittal.
Pattern generation and recognition in 3-D space have been of wide interest in fields varying from atomic and molecular structures
to organization of celestial objects. Our thoughts towards investigating patterns (and elucidation of geometrical rules) followed by
a known number of points with specific or specified coordinates are guided by the assumption/belief that there must be some
thermodynamic and/or kinetic basis leading to the organization of coordinates of the known points. In terms of biomolecular
structures, the DNA double helix is one of the strongest examples of such structures with coordinates of atoms in the phosphate
backbone and the side-chains provide a helical geometrical configuration. It needs to be appreciated (and emphasized) that,...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6038/version/1
Use of inadequate data and methodological errors lead to a dramatic overestimation of the water
footprint of Jatropha curcas
Provedor de dados: 17
Autores: Wouter Maes; Wouter M. J. Achten; Bart Muys.
In their recent article, Gerbens-Leenes et al. (1) calculated the water footprint (WF, the amount of water required to produce 1 GJ
of energy) of several bioenergy crops. One of the most remarkable findings of this study was the very high water footprint of this
species, which has serious management consequences. 

However, these results are in
apparent contrast with recent findings on this species. We present evidence that several errors were made by the authors when
calculating the water footprint of jatropha, which has lead to a dramatic overestimation. These errors include weaknesses
concerning the data used for the calculation of the water footprint, as well as flaws in the calculation method, as we demonstrate in
the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth & Environment; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3410/version/1
A Micropropagation, Molecular and Physiochemical Analysis of Canola Brassica napus L. From
Seeds
Provedor de dados: 17
Autores: Israr Khan; Muhammad Shuaib; Sikandar Khan; Muhammad Amir Zia; Haji Akbar.
Canola is important for its oil, anticarcinogenic phytochemicals and low saturated fat content and has wide scope for further
improvement through genetic transformations. Micropropagation is the practice of rapidly multiplying stock plant material to
produce a large number of disease free progeny plants, using modern asceptic plant tissue culture methods and also to conserve
germ plasm. A combination of auxin and cytikinin and a shock on growth regulator free medium is used for micropropagation.
Molecular and physiochemical analysis done to know variation in seeds protein content and electric conductivity.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4781/version/2
Pyrosequencing/Sanger Plant Genome Assembly (Limitations, Problems And Solutions) - On The
Way To Cucumber (Cucumis sativus L. cv. Borszczagowski) Draft Genome Sequence Publishing
Provedor de dados: 17
Autores: Rafal Woycicki; Zbigniew Przybecki.
Genome sequencing give us an opportunity to broaden the range and improve the quality of omics studies. New sequencing
technologies make it possible to achieve the sequencing reads fast and cheap. Since the assembly step of such next generation
reads is still not well standardized it is the most cumbersome part of sequencing projects.
We focus here on
the wide range of approaches undertaken to assemble the draft cucumber (Cucumis sativus L. cv. Borszczagowski) genome done
by using 12x coverage of pyrosequencing Titanium chemistry shotgun paired and unpaired reads combined with Sanger reads.
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4630/version/1
In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production
Provedor de dados: 17
Autores: Israr Khan; Muhammad Waheed Akktar; Ayaz Ali Khan.
According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic
chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to
improve activity, fidelity and generality of the enzyme.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6064/version/1
Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2
Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997 1,2. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60% 3.
The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases
4-7. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases
that lack an editing function, thereby resulting in antigenic drift 8. We report here the concurrent acquisition of the same
polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily
explained by the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/1
Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for
Composition Improvements
Provedor de dados: 17
Autores: I. C. Baianu; Valentin Prisecaru.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at
Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions. Conventional
“wet chemistry” analytical methods are time-consuming and costly. As a result, soybean breeders
and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive
method for composition analysis for academia and industry. Recent advancements in instrumentation design, such as the
application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly
improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/1
The BRIF: Success Factors and Crucial Considerations for Ensuring Impact.
Provedor de dados: 17
Autores: Lisa B. Miranda.
This presentation focused on the potential of the BRIF to capture data that demonstrates the initial and long term impact of
bioresources and those who direct/operate them for support/participation in biomedical and clinical research and their effects on
clinical care outcomes. The focus of this talk is also to share ideas and provide different ways to look at the BRIF. The BRIF can
be used to measure impact but may also be utilized to ensure and accelerate impact. Implementing the BRIF would potentially
lead to significant positive downstream effects. Many challenges exist for the biobanking area ahead of this. One central issue that
is delaying/impeding impact is the ability to create economically viable and sustainable resources.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Pharmacology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5877/version/1
Transient-mediated fate determination in a transcriptional circuit of HIV
Provedor de dados: 17
Autores: Leor Weinberger; Roy D. Dar; Michael Simpson.
Steady-state behavior and bistability have been proposed as mechanisms for decision-making in gene circuits. However, transient
gene expression has also been proposed to control cell fate with the decision arbitrated by the lifetime of the expression transient.
Here, we report that transcriptional positive-feedback plays a critical role in determining HIV infected cell-fate by extending the
duration of Tat expression transients far beyond what protein half-life modulation can achieve. To directly quantify feedback
strength and its effects on the duration of Tat transcriptional pulses, we exploit the noise inherent to gene-expression and measure
shifts in the autocorrelation of expression noise. The results indicate that transcriptional positive-feedback...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1198/version/1
*100 Semi-supercentenarians and older as a proposed sample set for the Archon Genomics X PRIZE
Validation Protocol*
Provedor de dados: 17
Autores: Thomas Perls; Nir Barzilai.
The
"Archon
Genomics
X
PRIZE
Validation
Protocol":http://precedings.nature.com/documents/5731/version/1 has been posted in part to solicit comments from the
scientific community. Here, we suggest 100 subjects age >=105 years as the source of DNAs for the
competition.

Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5756/version/1
Nuclear Medicine and Early Discovery of Disease
Provedor de dados: 17
Autores: I. C. Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer’s (AD) and Cancers in order to enable
their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death
with major consequences for the society.
Three major Nuclear Medicine techniques that are established for
diagnostic and research purposes are: Positron Emission Tomography (PET) CAT/CT and Nuclear Magnetic Resonance Imaging
(NMRI/MRI). These nuclear medicine techniques are discussed together with their clinical and research applications to early
diagnosis of Alzheimer's Disease (AD) and cancers. Novel approaches to early detection and modeling of AD and
cancers are then...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6196/version/1
Crosstalk and the spectrum of biological global broadcasts: Toward generalization of the Baars
consciousness model across physiological subsystems
Provedor de dados: 17
Autores: Rodrick Wallace.
Once cognitive biological phenomena are recognized as necessarily having 'dual' information sources, it is
easy to show that the information theory chain rule implies isolating coresident information sources from crosstalk requires more
metabolic free energy than permitting correlation. This provides conditions for an evolutionary exaptation leading to dynamic
global broadcasts of interacting cognitive biological processes analogous to, but slower than, consciousness, itself included within
the paradigm. The argument is closely analogous to the well-studied exaptation of noise to trigger stochastic resonance
amplification in physiological systems.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology; Molecular Cell Biology; Neuroscience; Evolutionary
Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6898/version/1
Underlying Mathematics in Diversification of Human Olfactory Receptors in Different Loci
Provedor de dados: 17
Autores: Sk. Sarif Hassan; Pabitra Pal Choudhury; Arunava Goswami.
As per conservative estimate, approximately (51-105) Olfactory Receptors (ORs) loci are present in human genome occurring in
clusters. These clusters are apparently unevenly spread as mosaics over 21 pair of human chromosomes. Olfactory Receptor (OR)
gene families which are thought to have expanded for the need to provide recognition capability for huge number of pure and
complex odorants. ORs form the largest known multi-gene family in the human genome. Recent studies have shown that 388 full
length and 414 OR pseudo-genes are present in these OR genomic clusters. In this paper, the authors report a classification
method for all human ORs based on their sequential quantitative information like presence of poly strings of nucleotides bases,
long range...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5475/version/1
The BRIF (Bioresource Research Impact Factor) as a tool for improving bioresource sharing in
biomedical research
Provedor de dados: 17
Autores: Laurence Mabile; Raymond Dalgleish; Gudmundur A. Thorisson; Mylène Deschênes; Robert
Hewitt; Jane Carpenter; Elena Bravo; Pierre-Antoine Gourraud; Paul Hofman; Jennifer Harris; Maria Angeles Munoz-Fernandes;
Anne Cambon-Thomsen.
The central aim of the BRIF (Bioresource Research Impact Factor) initiative is to construct a quantitative parameter to evaluate
bioresources, modeled to some degree on the Journal Impact Factor (JIF), and to provide guidance and methodology for
optimizing recognition of bioresources, their use and their sharing at international level. To implement this concept an
international working group has been set up. Specific tasks have been assigned to several sub-groups: BRIF digital identifier
schemes; BRIF parameters, measures and indicators; journal guidelines for resource citing and referencing; policies for
bioresource access and sharing. These are discussed in the present manuscript.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Pharmacology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6568/version/1
Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow
cytometry
Provedor de dados: 17
Autores: Vishal Gaurav; Amit Shavit; Susan C. Roberts.
Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to
comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular
metabolism is studied at the genomic level. Genomic stability in plant cell cultures is crucial as it affects cell growth and division,
metabolite accumulation and protein synthesis. A rapid and efficient method to prepare nuclei suspensions from aggregated cell
cultures of Taxus was employed. Methods were subsequently developed to simultaneously stain them for DNA and protein
content using Propidium Iodide and Fluorescein Isothiocyanate respectively. Flow cytometry was used to analyze and quantify the
DNA...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3809/version/1
Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2
Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60%. The
genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases. The
gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an
editing function, thereby resulting in antigenic drift. We report here the concurrent acquisition of the same polymorphism by
multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily explained by the
current...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/2
Category of Metabolic-Replication Systems in Biology and Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen
represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other
than the discrete topology of sets. Theoreticians of life's origins postulated that Life on Earth has begun with the
simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important
question of the minimal living organism by defining the functional relations that would have made life possible in such a minimal
system- a grandad and grandma of all living organisms on Earth.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Neuroscience; Bioinformatics; Earth & Environment; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7045/version/1
High efficiency transfection of thymic epithelial cell lines and primary thymic epithelial cells by
Nucleofection
Provedor de dados: 17
Autores: Richard T. O'Neil; Qiaozhi Wei; Brian G. Condie.
Thymic epithelial cells (TECs) are required for the development and differentiation of T cells and are sufficient for the positive
and negative selection of developing T cells. Although TECs play a critical role in T cell biology, simple, efficient and readily
scalable methods for the transfection of TEC lines and primary TECs have not been described. We tested the efficiency of
Nucleofection for the transfection of 4 different mouse thymic epithelial cell lines that had been derived from cortical or
medullary epithelium. We also tested primary mouse thymic epithelial cells isolated from fetal and postnatal stages. We found that
Nucleofection was highly efficient for the transfection of thymic epithelial cells, with transfection efficiencies of 30-70% for...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6283/version/1
Understanding Genomic Evolution of Olfactory Receptors through Fractal and Mathematical
Morphology
Provedor de dados: 17
Autores: Sk. Sarif Hassan; Pabitra Pal Choudhury; B. S. Dayasagar; Shantanav Chakraborty; Ranita Guha; Arunava Goswami.
Fractals and Mathematical Morphology are immensely used to study many problems in different branches of science and
technology including the domain of Biology. There are many more unrevealed facts and figures of genes and genome in
Computational Biology. In this paper, our objective is to explore how the evolutionary network is associated among Human,
Chimpanzee and Mouse with regards to their genomic information. We are about to explore their genomic evolution through the
quantitative measures of fractals and morphology. We have considered olfactory receptors for our case study. These olfactory
receptors do function in different species with subtle differences in the structures of DNA sequences. Those subtle differences can
be exposed through intricate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5674/version/1
Genome Replikin Count Predicts Increased Lethality of Cancer
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Those cancers, like thyroid and pancreatic cancer, with the lowest 5-year mortality rates, have Replikin Counts of about 20; lung
and brain cancers, with the highest 5-year mortality rates, have Replikin Counts of 275 and 325 respectively; the others fall in
between with approximate linearity. This is the first quantitative relationship of a genomic structure, Replikins, to 5-year mortality
rates.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7143/version/1
Without magic bullets: the biological basis for public health interventions against protein folding
disorders
Provedor de dados: 17
Autores: Rodrick Wallace.
Protein folding disorders of aging like Alzheimer's and Parkinson's diseases currently present intractable
medical challenges. 'Small molecule' interventions - drug treatments - often have, at best, palliative
impact, failing to alter disease course. The design of individual or population level interventions will likely require a deeper
understanding of protein folding and its regulation than currently provided by contemporary 'physics' or
culture-bound medical magic bullet models. Here, a topological rate distortion analysis is applied to the problem of protein folding
and regulation that is similar in spirit to Tlusty's (2010a) elegant exploration of the genetic code. The formalism
produces...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4847/version/2
Without magic bullets: the biological basis for public health interventions against protein folding
disorders
Provedor de dados: 17
Autores: Rodrick Wallace.
Protein folding disorders of aging like Alzheimer's and Parkinson's diseases currently present intractable
medical challenges. 'Small molecule' interventions - drug treatments - often have, at best, palliative
impact, failing to alter disease course. The design of individual or population level interventions will likely require a deeper
understanding of protein folding and its regulation than currently provided by contemporary 'physics' or
culture-bound medical magic bullet models. Here, a topological rate distortion analysis is applied to the problem of protein folding
and regulation that is similar in spirit to Tlusty's (2010a) elegant exploration of the genetic code. The formalism
produces...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4847/version/1
Unique features of Plasmids among different Citrobacter species
Provedor de dados: 17
Autores: Swapnil G. Sanmukh; Waman N. Paunikar.
The _Citrobacter_ plasmids are supposed to represent the host genetic association within the living bacterial cell. The plasmids
impart various beneficial characteristics to the host, helping it to retain suitable characteristics for adaptation as well as evolution.
The study aims at understanding the role of prophage in influencing host functional characteristics by horizontal gene transfer or
as whole plasmids. The _Citrobacter_ plasmid can be understood by analyzing many hypothetical protein sequences within its
genome. Our study included 82 hypothetical proteins in 5 _Citrobacter_ plasmids genomes. The function predictions in 31
hypothetical proteins and 3-D structures were predicted for 11 protein sequences using PS2 server. The probable function...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6836/version/1
Genomics and synthetic biology as a viable option to intensify sustainable use of biodiversity
Provedor de dados: 17
Autores: Elibio L. Rech.
The Amazon basin is an area of mega-biodiversity. Different models have been proposed^1-8^ for the establishment of an
effective conservation policy, increasing sustainability and adding value for biodiversity. Currently, a broad spectrum of
technologies from genomics to synthetic biology is available, and these permit the collection, manipulation and effective
evaluation of countless organisms, metabolic pathways and molecules that exist as potential products of a large, biodiverse
ecosystem. The use of Genomics and synthetic biology may constitute an important tool and be a viable option for the
prospection, evaluation and manipulation of biodiversity as advocated as well as be useful for developing methods for sustainable
use and the production of novel...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Earth & Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5759/version/1
Genome Replikin Count Predicts Increased Infectivity/Lethality of Viruses
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
The genomes of all groups of viruses whose sequences are listed on Pubmed, specimens since 1918, analyzed by a software from
Bioradar UK Ltd., contain Replikins which range in concentration from a Replikin Count (number of Replikins per 100 amino
acids) of less than 1 to 30 (see accompanying communications for higher Counts in tuberculosis, malaria, and cancer, associated
with higher lethality). Counts of less than 4.0 were found in ‘resting’ virus states; Counts greater than
4.0, found to be associated with rapid replication, were found invariably to accompany or to predict virus outbreaks, by as much
as two years, in viral hosts examined from salmon, to birds, to livestock, to humans. X-ray diffraction showed Replikins to be on
the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7144/version/1
Strong violation of the competitive exclusion principle
Provedor de dados: 17
Autores: Lev V. Kalmykov; Vyacheslav L. Kalmykov.
Bacteria and plants are able to form population waves as a result of their consumer behaviour and propagation. A soliton-like
interpenetration of colliding population waves was assumed but not proved earlier. Here we show how and why colliding
population waves of trophically identical but fitness different species can interpenetrate through each other without delay. We
have hypothesized and revealed here that the last mechanism provides a stable coexistence of two, three and four species,
competing for the same limiting resource in the small homogeneous habitat under constant conditions and without any fitness
trade-offs. We have explained the mystery of biodiversity mechanistically because (i) our models are bottom-up mechanistic, (ii)
the revealed...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Microbiology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6667/version/1
Genomic Replikin Counts of Infectious Salmon Anemia Virus (ISAV) in Canada Exceed the Counts in
Lethal Outbreaks in Norway, Chile, and Scotland. Real-Time Tracking of the Evolution of the ISAV
Genome and the Resultant Replikins Solid Phase ISAV Vaccine Make ISAV Pandemic Prevention
Possible.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Genomic Replikin CountsTM of Infectious Salmon Anemia Virus (ISAV) in Canada Exceed the Counts in Lethal Outbreaks in
Norway, Chile, and Scotland. Real-Time Tracking of the Evolution of the ISAV Genome and the Resultant Replikins Solid Phase
ISAV Vaccine Make ISAV Pandemic Prevention Possible.

Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6991/version/1
The Open Practises E-Science Network (OPEN)
Provedor de dados: 17
Autores: Cameron Neylon.
A grant proposal submitted for support to fund a research network focussed on identifying and dealing with the practical issues of
enabling open practise in research. The text of the proposal was written by a large number of people and coordinated by Cameron
Neylon.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1370/version/1
Protein and DNA evolution: Statistics and combinatorics are the rules of the game
Provedor de dados: 17
Autores: Arunava Goswami; Srimoyee Duttagupta; Chirantan Chakraborty; Amita Pal; Pabitra Pal Choudhury; Sk Sarif Hassan.
Let us assume AVMAYDRYVAIC or MAYDRYVAICCP, most important signature domain of
olfactory
receptor (OR) gene family assembled in evolution following simplest possible laws of statistics which says the addition of V after
A in AVMAYDRYVAIC or addition of A after M are independent events. There are 20 amino acids present in nature so the
probability
of
joint
occurrence
of
such
events
is
1/400.
Therefore
for
making
a
contiguous
string
of
AVMAYDRYVAIC or MAYDRYVAICCP, the probability would be 1 out of 2012. Over millions of
years of evolution this has certainly happened and that is why we see such a signature sequence in OR1D2, OR1D subfamily
member 2 as per "HORDE...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4859/version/1
Design of a dynamic model of genes with multiple autonomous regulatory modules by evolution in
silico
Provedor de dados: 17
Autores: Alexander V. Spirov; David M. Holloway.
A new approach to design a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico is
proposed. The approach is based on Genetic Algorithms, with new crossover operators especially designed for these purposes.
The approach exploits the subbasin-portal architecture of the fitness functions suitable for this kind of evolutionary modeling. The
effectiveness of the approach is demonstrated on a series of benchmark tests.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3913/version/1
Bio-resources for sharing: which communities and what specific needs
Provedor de dados: 17
Autores: Andres Metspalu.
Biobanks are an important source to obtain samples and data for research purposes for a wide array of communities. In fact,
sharing of bio-resources and data is an integral part of a biobank's role. For an effective partnership a list of
requirements or needs have to be met, assuring quality of the resources shared as well as quality of the collaboration in general.
Tracking and documenting bio-resource sharing and collaborations of a biobank can be seen as one indicator of the
"impact" a biobank has to the field. As an example, the practice of the Estonian Genome Center of the
University of Tartu is presented.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5659/version/1
BRIF Workshop Introduction
Provedor de dados: 17
Autores: Anne Cambon-Thomsen.
This presentation aims at introducing the place of BRIF in the landscape of data sharing, the BRIF concept, a presentation of the
working group, the ongoing debates and the workshop objectives.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5658/version/1
Can bacteria adapt to starvation-free environment?
Provedor de dados: 17
Autores: Kei Kitahara.
Bacteria will experience starvation-free environment if infinite nutrition is supplied continuously for a long period. In this study,
an evolutionary experiment was performed for 118 days where bacteria adapted to starvation-free environment and reduced their
doubling time. It is anticipated that this finding will help to select bacterial strains that can grow more rapidly in rich media.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7017/version/1
H5N1 Clade 2.2 Polymorphism Tracing Identifies Influenza Recombination and Potential Vaccine
Targets
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Elassai M. Labib; E Ayoub; Mona M. Aly; A-SA Arafa; Marshall R. Monteville;
Bruce R. Boynton; Jeffery Tjaden; Kenneth C. Earhart; Moustafa M. Mansour; Nasr M. ElSayed; A I. Nayei; A A. Abdelghani;
Hala M. Essmat.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997 1. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 2.
The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases
3.4. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that
lack an editing function, thereby resulting in antigenic drift 5. We traced polymorphism acquisition in Clade 2.2 sequences. We
report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, Clade 2.2 sub-clades in Egypt,
Russia...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/553/version/2
Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2
Sub-clades
Provedor de dados: 17
Autores: Henry Niman; Mona M. Aly; Abdel-Satar Arafa; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M.
Esmat; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997 1,2. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 3.
The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases
4-7. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases
that lack an editing function, thereby resulting in antigenic drift 8. We report here the concurrent acquisition of the same
polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, Kuwait, and Ghana. These changes are not
easily...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/459/version/4
Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2
Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Mona M. Aly; Jeffery Tjaden; Kenneth C. Earhart; Marshall R. Monteville;
Moustafa M. Mansour; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M. Esmat; Emad M. Labib; Ehab A.
Ayoub; Abdelattar Arafa; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60%. The
genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases. The
gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an
editing function, thereby resulting in antigenic drift. We report here the concurrent acquisition of the same polymorphism by
multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily explained by the
current...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics & Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/3
Aggregation of Single Nucleotide Polymorphisms in a Human H5N1 Clade 2.2 Hemagglutinin
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Jeffery Tjaden; Kenneth C. Earhart; Marshall R. Monteville; Mona M. Aly; Moustafa
M. Mansour; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M. Esmat; Emad M. Labib; Ehab A. Ayoub;
Abdelattar Arafa; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
The evolution of H5N1 has attracted significant interest 1-4 due to linkages with avian 5,6 and human infections 7,8. The basic
tenets of influenza genetics 9 attribute genetic drift to replication errors caused by a polymerase complex that lacks a proof
reading function. However, recent analysis 10 of swine influenza genes identifies regions copied with absolute fidelity for more
than 25 years. In addition, polymorphism tracing of clade 2.2 H5N1 single nucleotide polymorphisms identify concurrent
acquisition 11 of the same polymorphism onto multiple genetic backgrounds in widely dispersed geographical locations. Here
we show the aggregation of regional clade 2.2 polymorphisms from Germany, Egypt, and sub-Sahara Africa onto a human
Nigerian H5N1...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/743/version/1
12x coverage 454/Sanger Hybrid Assembly of Cucumber (Cucumis sativus L. cv.Borszczagovski)
genome - the most efficient way to begin post genomic era
Provedor de dados: 17
Autores: Rafał Wóycicki; Justyna Witkowicz; Magdalena Pawełkowicz; Ewa Siedlecka;
Wojciech Gutman; Wojciech Pląder; Anna Seroczyńska; Mieczysław Śmiech;
Katarzyna Niemirowicz-Szczytt; Stanisław Karpiński; Stefan Malepszy; Zbigniew Przybecki.
New sequencing technologies make it possible to achieve genome sequence fast and cheap. Since the assembly step of such next
generation reads is still not well standardized it is the most cumbersome part of sequencing projects. 
We
present here approaches undertaken on the way to the draft assembly the cucumber (Cucumis sativus L. cv. Borszczagowski)
genome done with the use of 8x unpaired and 4x paired (3 Kbp) pyrosequenced 454 XLR Titanium reads, together BAC library
ends fragments (12,7x physical coverage). Two different assembly approaches, namely Celera and Celera/Arachne were finally
used. The Celera result was 15’196 contigs of 197 Mbp length and N50 27’086 bp and
4’157 scaffolds of 224 Mbp length...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5466/version/1
H5N1 Clade 2.2 Polymorphism Tracing Identifies Influenza Recombination and Potential Vaccine
Targets
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Bruce R. Boynton; Jeffery Tjaden; Kenneth C. Earhart; Moustafa M. Mansour; Nasr
M. ElSayed; A I. Nayei; A A. Abdelghani; Hala M. Essmat; Elassai M. Labib; E Ayoub; Mona M. Aly; A-SA Arafa; Marshall R.
Monteville.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong
Kong in 1997 1. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 2.
The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases
3.4. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that
lack an editing function, thereby resulting in antigenic drift 5. We traced polymorphism acquisition in Clade 2.2 sequences. We
report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, Clade 2.2 sub-clades in Egypt,
Russia...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/553/version/1
Beyond the fuzzy lock-and-key: spontaneous symmetry shifts and glycan/lectin logic gates
Provedor de dados: 17
Autores: Rodrick Wallace.
Changes in the molecular topology of glycan/lectin interaction may explain observed reaction punctuation driven by experimental
gradients in reactant concentration. Adoption of a 'biological renormalization' perspective from statistical
physics for the analysis of such phase transitions suggests, in marked contrast to conventional physical systems, a broad spectrum
of possible universality class behaviors. This spectrum may, in typical perverse biological manner, be of central scientific interest.
Generalization, via formalism abducted from coevolutionary theory, suggests that glycan/lectin molecular switches instantiate
logic gates that may be as sophisticated as those characterizing basic neural process, if on a different scale.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Molecular Cell Biology; Pharmacology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6849/version/1
Design of a dynamic model of genes with multiple autonomous regulatory modules by evolution in
silico
Provedor de dados: 17
Autores: Alexander V. Spirov; David M. Holloway.
A new approach to design a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico is
proposed. The approach is based on Genetic Algorithms, with new crossover operators especially designed for these purposes.
The approach exploits the subbasin-portal architecture of the fitness functions suitable for this kind of evolutionary modeling. The
effectiveness of the approach is demonstrated on a series of benchmark tests.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/3913/version/2
Mobile Image Ratiometry for the Detection of Botrytis cinerea (Gray Mold)
Provedor de dados: 17
Autores: Donald C. Cooper.
Mobile Platform Informatics (MPI) and Smartphone Informatics (SPI) methods like Mobile Image Ratiometry (MIR) are
potentially transformative point-of-use instantaneous analysis tools that are useful across a variety of industries. In agriculture,
MIR-compatible immuno test strips allow early detection of a number of biotic stressors before devastating crop losses occur.
Here
we
describe
a
low-cost
and
easy-to-use
Smartphone
and/or
tablet-based
protocol (Mobile
Assay Inc.,
www.mobileassay.com) for the detection and on-sight instantaneous analysis of B. cinerea, a fungus that causes significant
damage to a variety of plants and flowers. Early detection and tracking of the B. cinerea fungus before the visible gray mold
appears has the potential to increase...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth & Environment; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6989/version/1
Comments and Suggestions for Improvement of the Archon Genomics X PRIZE Validation Protocol
Provedor de dados: 17
Autores: Alexander Wait Zaranek; Tom Clegg; Ward Vandewege; Joseph V. Thakuria.
This document is a comment on the X PRIZE validation protocol written by Kedes et al. (2011).
We propose several
modifications which we think will improve the fairness and transparency of the contest while keeping the cost of the validation
process under control.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5766/version/1
Tuning and regulating the repertoire of glycan determinants
Provedor de dados: 17
Autores: Rodrick Wallace; Deborah Wallace.
We apply Tlusty's information-theoretic index theorem analysis of the genetic code to the glycome, using a cognitive
paradigm by which external information sources constrain and tune the glycan code error network, in the context of available
metabolic energy. The model suggests spontaneous symmetry breaking of the glycan code as a function of metabolic energy
intensity, an effect that may be currently present, or embedded in evolutionary trajectory, recording large-scale ecosystem
resilience shifts in energy availability such as the aerobic transition. Once focused on a subset of the glycan error code network
however, the glycan production machinery must then be regulated by an elaborate cognitive process to ensure that what is
produced matches...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Molecular Cell Biology; Pharmacology; Evolutionary
Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5932/version/2
Cloning and expression of first gene for biodegrading microcystins by Sphingopyxis sp. USTB-05
Provedor de dados: 17
Autores: Hai Yan; Junfeng Wang; Jian Chen; Wei Wei; Hui Wang.
Harmful cyanobacterial blooms (HCBs) in natural waters are a growing environmental problem worldwide because microcystins
(MCs) produced by cyanobacteria are potent hepatotoxins and tumor promoters. MCs are resistant against physical and chemical
factors. Thus, biodegradation is the most efficient method for removing MCs, and a number of bacterial strains, especially genus
_Sphingomonas_, have been isolated for biodegrading MCs. Although the pathway, enzyme, and gene for biodegrading MCs by
_Sphingomonas sp._ have been widely identified recently, no gene concerned with the biodegradation of MCs has been
successfully cloned and expressed. In this study, we show that the first and most important gene of mlrA, containing 1,008 bp
nucleotides in length, in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Earth & Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4846/version/1
A novel mathematical tool for generating highly conserved protein domain via different organismal
genomic landscapes
Provedor de dados: 17
Autores: Arunava Goswami; Pabitra Pal Choudhury; Rajneesh Singh; Sk. Sarif Hassan.
Darwinian evolution hypothesizes that a short stretch of DNA was first constructed and then it expanded to give rise to a long
strand. This long strand then produced a mix of exons, introns and repetitive DNA sequence. The order of production of above
three kinds of DNA sequence is unknown. Reshuffling of stretches of DNA like above within organisms has given rise to different
chromosomes. Till date it is not known how this process is governed. In this paper we show that starting with a sixteen base-pair
human olfactory DNA sequence one can form a highly conserved protein domain. Once this domain is formed repetitive DNA
sequences of a particular kind starts generating which signifies that this particular conserved protein domain will be unique in
nature....
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4850/version/1
Cocaine self-administration in the mouse: A low-cost, chronic catheter preparation
Provedor de dados: 17
Autores: Matthew B. Pomrenze; Michael V. Baratta; Brian A. Cadle; Donald C. Cooper.
Intravenous drug self-administration is the most valid animal model of human addiction because it allows volitional titration of the
drug in the blood based on an individual’s motivational state together with the pharmacokinetic properties of the
drug. Here we describe a reliable low-cost mouse self-administration catheter assembly and protocol that that can be used to
assess a variety of drugs of abuse with a variety of protocols. We describe a method for intravenous catheter fabrication that
allows for efficient and long-lasting intravenous drug delivery. The intravenous catheters remained intact and patent for several
weeks allowing us to establish stable maintenance of cocaine acquisition. This was followed by a dose response study in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/7040/version/1
Prediction of specific virus outbreaks made from the increased concentration of a new class of virus
genomic peptides, replikins.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Advance warning of pathogen outbreaks has not been possible heretofore. A new class of genomic peptides associated with rapid
replication was discovered and named replikins. Software was designed to analyze replikins quantitatively. Replikin concentration
changes were measured annually prior to, and “real time” every few days during, the 2009 H1N1
influenza pandemic. Replikins were seen by both linear sequence representation and three-dimensional X-ray diffraction, and
found
to
expand
on
the
virus
hemagglutinin
surface
prior
to
and
during
the
H1N1
pandemic.

A highly significant increased concentration of virus replikins was found
a) retrospectively in three pandemics from 1918 to 1999 (14,227...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6279/version/1
Increasing Access To Bioinformatics Resources: Increase Community Curation by Increasing Your
Community
Provedor de dados: 17
Autores: Jennifer M. Williams.
The amount of biological data has risen exponentially over the last decade. Along with this rise, the number and types of
bioinformatics resources has risen such that the sheer number of bioinformatics resources is overwhelming. For these resources to
attain their full potential, they must be efficiently and extensively utilized. But in such a plethora of resources, how does a
researcher new to a field find the resources that will meet their needs. Once a resource is found, how does the researcher quickly
learn to utilize that resource fully? There are resource lists such as those provided by the journal Nucleic Acid Research (NAR),
BioMed Central and the Univ. of Pittsburgh Health Sciences Library, and most resources include their own documentation. But...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3163/version/1
Designing L-Systems for making three and six open reading frames from the leading strand of a single
DNA molecule
Provedor de dados: 17
Autores: Arunava Goswami; Rajneesh Singh; Pabitra Choudhury; Sk. Sarif Hassan Hassan.
Current molecular biology dogma (except alternative splicing) suggests that leading strand of DNA produces mRNA and usually
the leading strand gives one ORF (ORF) among the available six ORFs. We attempted to break this rule where three and six ORFs
from the leading strand will be formed by designing L-Systems, originally proposed to study symmetry of biological world. We
thought this would revolutionize the world of synthetic biology. While doing so, we found L-System containing
A→T, T→G, C→A, and G→C, i.e., single nucleotide production rule will stop
growing after one nucleotide. L-System containing either (a) A→TG, T→GC, C→GA,
G→TC or (b) A→CTG,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4844/version/1
An attempt to understand Barstar, Barnase and Olfactory receptor protein folding problems using
mathematical biological approach
Provedor de dados: 17
Autores: Arunava Goswami; Sk Sarif Hassan; Pabitra Pal Choudhury.
Protein folding problem as attracted structural biologists immensely (1). Till date, correlation between X-ray crystallographic and
NMR data are considered to be the best methods for determining structure of intra-cellular proteins. Generating crystals and
finding correct experimental conditions for NMR are largly a gamble and resultant data processing highly time consuming. Even
if, a large number of laboratories around the world and India make crystals of protein but majority of them fail to generate
crystallographic data less than or equal to 10A resolution with currently available instruments. This means that X-ray
crystallographic data have been generated from protein crystals with non-uniform lattices. We believe that a large portion of
(chain of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4891/version/1
Open Data in Science
Provedor de dados: 17
Autores: Peter Murray-Rust.
Open Data (OD) is an emerging term in the process of defining how scientific data may be published and re-used without price or
permission barriers. Scientists generally see published data as belonging to the scientific community, but many publishers claim
copyright over data and will not allow its re-use without permission. This is a major impediment to the progress of scholarship in
the digital age. This article reviews the need for Open Data, shows examples of why Open Data are valuable and summarizes
some early initiatives in formalizing the right of access to and re-use of scientific data.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1526/version/1
Computational system biology
Provedor de dados: 17
Autores: Ankit Geete.
Systems biology is a multi-disciplinary field that deals with mechanisms involved in complex biological processes
by considering them as integrated systems of multiple interacting components. The huge amount of data involved in this study
necessitates the use of computational tools.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7104/version/1
Reflect: Augmented Browsing for the Life Scientist
Provedor de dados: 17
Autores: Evangelos Pafilis; Seán I. O'Donoghue; Lars J. Jensen; Heiko Horn; Michael Kuhn; Nigel P.
Brown; Reinhard Schneider.
Anyone who regularly reads life science literature often comes across names of genes, proteins, or small molecules that they
would like to know more about. To make this process easier, we have developed a new, free service called Reflect
("http://reflect.ws":http://reflect.ws) that can be installed as a plug-in to Firefox or Internet Explorer.
Reflect tags gene, protein, and small molecule names in any web page, typically within a few seconds, and without affecting
document layout. Clicking on a tagged gene or protein name opens a popup showing a concise summary that includes synonyms,
database identifiers, sequence, domains, 3D structure, interaction partners, subcellular location, and related literature. Clicking on
a tagged small...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3212/version/1
Statistical Tests for Detecting Differential RNA-Transcript Expression from Read Counts
Provedor de dados: 17
Autores: Oliver Stegle; Philipp Drewe; Regina Bohnert; Karsten Borgwardt; Gunnar Rätsch.
As a fruit of the current revolution in sequencing technology, transcriptomes can now be analyzed at an unprecedented level of
detail. These advances have been exploited for detecting differential expressed genes across biological samples and for
quantifying the abundances of various RNA transcripts within one gene. However, explicit strategies for detecting the hidden
differential abundances of RNA transcripts in biological samples have not been defined. In this work, we present two novel
statistical tests to address this issue: a 'gene structure sensitive' Poisson test for detecting differential
expression when the transcript structure of the gene is known, and a kernel-based test called Maximum Mean Discrepancy when it
is unknown. We...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4437/version/1
Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of
prostate cancer and contribute to pathogenesis of multiple common human disorders
Provedor de dados: 17
Autores: Anna Glinskii; Shuang Ma; Jun Ma; Denise Grant; Chang-Uk Lim; Ian Guest; Stewart Sell; Ralph Buttyan; Gennadi
Glinsky.
Biological and mechanistic relevance of intergenic disease-associated genetic loci (IDAGL) containing highly statistically
significant disease-linked SNPs remains unknown. Here we present the experimental and clinical evidence revealing important
role of IDAGL in human diseases. Targeted RT-PCR screen coupled with sequencing of purified PCR products detects
widespread transcription at multiple intergenic disease-associated genomic loci (IDAGL) and identifies 96 small non-coding
trans-regulatory RNAs of ~ 100-300 nt in length containing SNPs associated with 21 common human disorders (snpRNAs).
Functionality of snpRNAs is supported by multiple independent lines of experimental evidence demonstrating their
cell-type-specific expression and evolutionary...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6069/version/1
Visual Molecular Dynamics Investigations of the Impact of Hydrophobic Nanoparticles on Prognosis
of Alzheimer’s Disease and Cancers
Provedor de dados: 17
Autores: I. C. Baianu; M Charles; V. I. Prisecaru.
The possible impact of hydrophobic lectin nanoparticles on the prognosis and progression of Alzheimer's disease
(AD) and cancers was investigated by Visual Molecular Dynamics (VMD) computer modeling programs available from the
Beckmann Advanced Research Institute at the University of Illinois at Urbana. Our results indicate the possibility of impeding
pathological aggregation of certain proteins such as modified tau- or beta-amyloid that are currently being considered as possible
causes of Alzheimer's disease. VMD programs serve as useful tools for investigation hydrophobic protein aggregation
that may play a role in aging of human populations.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7111/version/1
On the social induction of Alzheimer's disease: An index theorem aging model for amyloid
formation
Provedor de dados: 17
Autores: Rodrick Wallace; Deborah Wallace.
The central 'risk factor' for Alzheimer's disease (AD) is age. From first principles, we
construct a mathematical model of protein folding and its in vivo regulation that gives this result in a natural manner. We extend
the basic approach using topological information theory methods, and examine a case history of socially-induced premature aging
in the United States.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6012/version/1
Detection of substrate binding motifs for morphine biosynthetic pathway intermediates in novel
wound inducible (R,S)-reticuline 7-O-methyltransferase of Papaver somniferum
Provedor de dados: 17
Autores: Sonal Mishra; Abha Meena; Sema Singh; Dharmendra Kumar Yadav; Feroz Khan; Rakesh K. Shukla.
The benzylisoquinoline alkaloids (BIA) comprise a large and diverse group of nitrogen-containing secondary metabolites with
about 2500 compounds identified in plants. BIA biosynthesis begins with the condensation of the tyrosine derived precursors
dopamine and p-hydroxyphenylacetaldehyde to (S)-norcoclaurine. Subsequent regiospecific O- and N-methylations and aromatic
ring hydroxylation lead to (S)-reticuline, which is the central intermediate for almost all BIAs. For morphinan alkaloid
biosynthesis, (S)-reticuline undergoes an inversion of stereochemistry to (R)-reticuline, followed by C-C phenol coupling
catalyzed by a unique cytochrome P450-dependent monooxygenase to yield salutaridine. The cDNA sequence of enzymes leading
to (S)-reticuline, as well as...
Tipo: Poster
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5790/version/1
DNA^+Pro^: an Improved Progressive Multiple Sequence Alignment Algorithm for Evolutionary
Analysis Using Combined DNA-Protein Sequences
Provedor de dados: 17
Autores: Xiaolong Wang; Shuang-yong Xu; Deming Gou.
Alignment of DNA and protein sequences is a basic tool in the study of evolutionary, structural and functional relationship among
macromolecules. Present sequence alignment methods are somewhat error-prone, often producing systematic bias. Errors in
sequence alignments sometimes lead to subsequent misinterpretation of evolutionary, structural and functional information in
genes, proteins and genomes. In traditional sequence alignment algorithms, alignments of DNA and protein sequences are
conducted separately. It has been long believed that the phylogenetic signal disappears more rapidly from DNA sequences than
from encoded proteins. It is therefore generally preferable to align sequences at the amino acid level. Here we present a new...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4898/version/1
Lost in translation: Toward a formal model of multilevel, multiscale medicine
Provedor de dados: 17
Autores: Rodrick Wallace.
For a broad spectrum of low level cognitive regulatory and other biological phenomena, isolation from signal crosstalk between
them requires more metabolic free energy than permitting correlation. This allows an evolutionary exaptation leading to dynamic
global broadcasts of interacting physiological processes at multiple scales. The argument is similar to the well-studied exaptation
of noise to trigger stochastic resonance amplification in physiological subsystems. Not only is the living state characterized by
cognition at every scale and level of organization, but by multiple, shifting, tunable, cooperative larger scale broadcasts that link
selected subsets of functional modules to address problems. This multilevel dynamical viewpoint has implications...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Immunology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6973/version/1
Applying of novel subtraction method Genetically Directed Differential Subtraction Chain (GDDSC)
in plant genomes
Provedor de dados: 17
Autores: Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawełkowicz; Rafał
Wóycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically
Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate
tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring
plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber
plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified
tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for
requested traits.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5465/version/2
Archon Genomics X PRIZE Validation Protocol
Provedor de dados: 17
Autores: Larry Kedes; Granger Sutton; Edison Liu; Victor Jongeneel.
This document is a collective assembly of techniques designed to test the quality and accuracy of 100 whole human genome
sequences resulting from the $10 Million Archon Genomics X PRIZE (AGXP) competition. The purpose of this article is to enlist
constructive criticism from the genomic and genetic community on the outlined approaches. The intent for the final version of this
Validation Protocol is to become a useful standard by which to gauge the capabilities of whole genome sequencing technologies
that emerge even after 2012.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5731/version/1
The interplay of descriptor-based computational analysis with pharmacophore modeling builds the
basis for a novel classification scheme for feruloyl esterases
Provedor de dados: 17
Autores: D.B.R.K. Gupta Udatha; Irene Kouskoumvekaki; Lisbeth Olsson; Gianni Panagiotou.
One of the most intriguing groups of enzymes, the feruloyl esterases (FAEs), is ubiquitous in both simple and complex organisms.
FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of
substrates for cleaving ester bonds and synthesizing high-added value molecules through esterification and transesterification
reactions. During the past two decades extensive studies have been carried out on the production and partial characterization of
FAEs from fungi, while much less is known about FAEs of bacterial or plant origin. Initial classification studies on FAEs were
restricted on sequence similarity and substrate specificity on just four model substrates and considered only a handful of FAEs...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4762/version/1
Genetically modified Cotton species detection by LISS-III satellite data
Provedor de dados: 17
Autores: Saumitra Mukherjee; Satyanarayan Shashtri; Chander Kumar Singh; Bindu Kumari; Reena Kumari; Ram Avatar;
Anita Mukherjee; Bhoop Singh.
It is possible to infer the genetically modified species by using remotely sensed data. Using ERDAS software the algorithm of BT
(Bacillus thuringiensis) Cotton in Punjab, India was developed successfully. GPS enabled space technology has the potential to
identify the exact location of Bt Cotton by generating Normalized Difference Vegetation Index (NDVI) for the calculation of total
area covered by this species. It was possible to develop a correlation in between genetically modified Cotton crop and NDVI
value. In parts of Bhatinda district of Punjab the yield of Bt Cotton and NDVI showing R2 value of more than 4.5 in regression
analysis. A correlation matrix was also generated which shows that NDVI values of BT cotton has reasonably acceptable
correlation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Earth & Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/2659/version/1
TATA binding protein binds to single stranded DNA: A new model for eukaryotic transcription
Provedor de dados: 17
Autores: Rostem J. Irani.
Although the players mediating the initiation of eukaryotic transcription appear to be known, the mechanism is not. In this paper
we report findings on the interaction between the TATA box binding protein (TBP) and DNA, using a novel optical technology,
that point to a potential plausible physical mechanism, and at the same time tie together a number of otherwise disparate
observations. TBP plays a central and ubiquitous role in initiating eukaryotic transcription, binding to promoters with and without
the canonical 5’ TATA(A/T)A(A/T)(G/A) 3’ motif1. It also tends to bind to double-stranded DNA
promiscuously, and though affinity is lower, the number of binding opportunities is greater. TBP-DNA complexes have been
characterized...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4139/version/1
Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC)
in plant genomes
Provedor de dados: 17
Autores: Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawełkowicz; Rafał
Wóycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically
Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate
tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring
plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber
plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified
tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for
requested traits.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5465/version/1
Heterogeneity in Ty1-copia group of retroelements in chickpea (Cicer arietinum) genome
Provedor de dados: 17
Autores: Manoj K. Rajput; Kailash C. Upadhyaya.
Retrotransposons constitute a major fraction of plant genomes and these elements may have played a significant role in evolution
and sequence organization of genomes. In order to access the diversity of Ty1-copia group of retroelements, reverse transcriptase
(RT) sequences were amplified from chickpea genome, using the primers derived from two conserved domains of RT region.
Thirty-six RT regions from independent amplicons were cloned and sequenced. On the basis of homology of deduced amino
acids, the RT sequences could be grouped into three major families. The intra-family divergence at amino acid level ranges from 2
to 19%. Though intra-family RT sequences were conserved but no two sequences were identical. The results indicate a high
degree of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/697/version/1
Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and
Embryos
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Doina M. Costescu.
Novel
methodologies
are
currently
being
evaluated
for
the
Chemical
Analysis
of
soybean seeds as well as developing 
mature soybean embryos by FT-IR/NIR
Microspectroscopy. This is the first report of FT-NIR Chemical Images obtained with 1 micron resolution. NIR spectra of
Proteins, Oil and DNA fibers are obtained for regions as small as 1μ^2^.
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6591/version/1
Subtyping of Dengue Viruses using Return Time Distribution based Appproach
Provedor de dados: 17
Autores: Pandurang Kolekar; Mohan Kale; Urmila Kulkarni-Kale.
Dengue virus (DENV) is the causative agent of Dengue Hemorrhagic Fever and Dengue Shock Syndrome, and continues to
represent a major public health hazard. DENVs are antigenically classified in four serotypes and each serotype is further divided
into respective genotypes. The association between DENV subtypes and the kind & severity of disease caused by them
is known. Experimental and computational approaches for subtyping are routinely used for the purpose of diagnosis and treatment
of DENV, in addition to the study of phylodynamics. All virus-specific molecular subtyping tools make use of sequence
alignments at backend. But as the volume of molecular data increases, alignment-dependent methods become computationally
intensive. Hence, the need for...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5590/version/1
DNA expression microarrays may be the wrong tool to identify biological pathways
Provedor de dados: 17
Autores: Adrian Mondry; Marie Loh; Alessandro Giuliani.
DNA microarray expression signatures are expected to provide new insights into patho- physiological pathways. Numerous
variant statistical methods have been described for each step of the signal analysis. We employed five similar statistical tests on
the same data set at the level of gene selection. Inter-test agreement for the identification of biological pathways in BioCarta,
KEGG and Reactome was calculated using Cohen’s k- score. The identification of specific biological pathways
showed only moderate agreement (0.30 < k < 0.79) between the analysis methods used.
Pathways
identified by microarrays must be treated cautiously as they vary according to the statistical method used.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1036/version/1
Nurturing the seeds of success in the semi-arid tropics
Provedor de dados: 30
Palavras-chave: Bioinformatics; Chickpeas; Pigeonpeas; Sorghum; Mycotoxins; Millets; Hybrids; Groundnuts; Biotechnology;
Transgenics.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3270
In vitro organogenesis from internodal segments of adult sweet orange plants
Provedor de dados: 86
Autores: Bassan,Meire Menezes; Mourão Filho,Francisco de Assis Alves; Miyata,Luzia Yuriko; Mendes,Beatriz Madalena
Januzzi.
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and
'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of
6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour
photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and
Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Tipo: Journal article
Palavras-chave: Citrus sinensis; Adventitious buds; Biotechnology; Plant growth regulators.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600015
IMPACTS OF GROWTH STIMULANTS IN THE DOMESTIC LIVESTOCK SECTOR
Provedor de dados: 31
Autores: Peterson,
Everett B.; Preckel,
Paul V.; Hertel,
Thomas W.; McGuirk,
Anya M.
Many individuals are predicting a "second green revolution" in agriculture from the introduction of growth stimulants into the
livestock industries. An economy-wide approach is used to determine the affects on prices and quantities of introducing growth
stimulants in the domestic dairy and pork industries. In general, the impacts are found to be much smaller than previous research
focused at the sectoral level has suggested. Increases in the amount of lean meat per hog, which reduces processor demand, may
actually lead to a reduction in pork production at the farm-level.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; General equilibrium; Pork and dairy; Livestock Production/Industries.
Ano: 1992
URL: http://purl.umn.edu/116106
Knowledge Capital, Intangible Assets, and Leverage: Evidence from U.S. Agricultural Biotechnology
Firms
Provedor de dados: 31
Autores: Sporleder,
Thomas L.; Moss,
Leeann E.
Agricultural biotechnology firms are high technology companies. Firms in general, and high technology firms in particular, are a
set of both assets in place and growth opportunities. This has important implications for managerial decision-making.
Knowledge capital motivates exploitation of growth options, which affects firm cash flow. In turn, the level and volatility of firm
cash flow influences firm financing decisions. Previous studies suggest that knowledge capital can influence both the location and
capital structure of firms in the biotechnology industry. However, empirical analysis has not extended to agricultural
biotechnology firms. This research helps in understanding the role of knowledge capital and other intangible assets in capital
structure...
Tipo: Journal Article
Palavras-chave: Capital structure; Agricultural biotechnology; Knowledge capital; Intangible assets; Agribusiness.
Ano: 2004
URL: http://purl.umn.edu/8123
Biotechnology and the Development of Food Markets: Retrospect and Prospects
Provedor de dados: 31
Autores: Moschini,
GianCarlo.
Biotechnology has had an important impact on the agricultural and food industries over the last twelve years by way of fast and
extensive adoption of a few genetically modified (GM) crops. This has produced large efficiency gains, including higher yields
and reduced costs of weed and pest control, as well as some environmental benefits. The expected development of crops with
additional agronomic traits, and with output traits to improve the nutrition and health attributes of food products, holds the
potential for even more pervasive impacts. Full realisation of such promises may require overcoming the constraining effects of
restrictive GM product regulations.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetically modified products; Innovation; Regulation; Research and development.
Ano: 2008
URL: http://purl.umn.edu/42811
Risk assessment and management of genetically modified organisms under Australia's Gene
Technology Act
Provedor de dados: 31
Autores: Linacre,
Nicholas A.; Falck-Zepeda,
Jose Benjamin; Komen,
John; MacLaren,
Donald.
Compared to both Canada and the United States, Australia has been slow to approve commercial planting of transgenic crops.
Two probable reasons exist for the slow approval rate of transgenic crops in Australia. The first reason is community perceptions
about the risks associated with transgenic technologies. The second is the regulatory framework currently employed to approve
commercial releases. This paper examines some of the potential regulatory issues that may be affecting the review process and
approval of transgenic technologies. First we provide a brief introduction to the regulatory structure in Australia, second we
consider the impact of regional, national and state jurisdictions, third we argue that the regulator needs to consider the use of...
Tipo: Report
Palavras-chave: Risk assessment; Biotechnology; Environmental risk; Crop Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55414
CHINA'S REGULATION OF BIOTECHNOLOGY - DOES IT CONFORM TO THE WTO?
Provedor de dados: 31
Autores: Loppacher,
Laura J.; Kerr,
William A.
The People's Republic of China has been working for over two decades to develop a domestic biotechnology industry. Even
though China is a developing country, it is set to become a significant international player in the field of biotechnology. China's
accession to the World Trade Organization in December of 2001 means that China's trade and regulatory regime should now
reflect its WTO commitments. This paper outlines the development of the Chinese biotechnology industry and how it may evolve
in the future. It assesses China's current approach to trade and regulation of biotechnology in relation to GATT rules on trade in
goods and the TRIPS commitments pertaining to the protection of intellectual property. The conclusion is that China's trade and...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; China; Intellectual property; WTO; International Relations/Trade.
Ano: 2004
URL: http://purl.umn.edu/23938
Botânica: uma ciência básica ou aplicada?
Provedor de dados: 88
Autores: AZEVEDO,JOÃO LÚCIO.
Exemplos de interações entre microrganismos endofíticos e suas plantas hospedeiras são usados para demonstrar a importância da
Botânica no desenvolvimento da biotecnologia e para a preservação da biodiversidade. Como conclusão, uma visão holística da
ciência atual torna irrelavante qualquer distinção entre aspectos básicos e aplicados da ciência, principalmente quando se
consideram as ciências biológicas, como é o caso da Botânica.
Tipo: Journal article
Palavras-chave: Biotechnology; Biodiversity; Plant-microbes interactions; Endophytes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000500002
Biotechnology applied to salmoniculture
Provedor de dados: 96
Autores: Isler,Iván Valdebenito.
This work presents a conceptual analysis of the main biotechnologies used in Chilean salmoniculture, which is based on the
production of Atlantic salmon (Salmo salar, 356.407 t),silver salmon or coho (Oncorhynchus kisutch, 116.481 t), (O. tshawytscha,
2.062 t) and rainbow trout (O. mykiss, 189.178 t).These activities are focused on the photoperiod artificial manipulation to obtain
out-of-season spawning, in the use of hormonal therapies which allow synchronizing the final oocyte maturation (FOM) and
sexual maturity acceleration or the increase in milt volume produced by males. Such actions are carried out using GnRHa in doses
close to 10 µg/kg of fish. Once sexual maturity is reached, in vitro manipulation of gamets must often be done due to either the...
Tipo: Journal article
Palavras-chave: Biotechnology; Induced spawning; Photoperiod; Reproduction; Sex manipulation; Thermoperiod.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300004
Embryogenic calli induction from nucellar tissue of Citrus cultivars
Provedor de dados: 63
Autores: Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Demétrio,Clarice Garcia Borges;
Mendes,Beatriz Madalena Januzzi.
Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media
tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT
macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA
[EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins
(Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia'
sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L.
Osbeck) produced hard...
Tipo: Journal article
Palavras-chave: Biotechnology; Tissue culture; Cultivar improvement; Growth regulator; Protoplast.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012
Seed technology training in the year 2000
Provedor de dados: 63
Autores: McDonald,M.B.; Marcos-Filho,J.; Bino,R.J.
Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture
driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication
technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new
approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this
objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic
advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology...
Tipo: Journal article
Palavras-chave: Seed quality; Education; Seed industry; Biotechnology; Seed enhancements; Consortium.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500001
Trust in Scientists and Food Manufacturers: Implications for the Public Support of Biotechnology
Provedor de dados: 31
Autores: James,
Harvey S., Jr.
An analysis of data from the U.S. Biotechnology Study, 1997S1998 reveals that few variables affect public trust in scientists,
while variables representing perceived benefits, risks, trustworthiness, and competence affect trust in food manufacturers on
matters of biotechnology. Both trust in scientists and trust in food manufacturers have a large and important effect on public
support for biotechnology, although trust in scientists is found to be more important than trust in food manufacturers. Findings of
this analysis suggest that trust in scientists is relatively generalized, while trust in food manufacturers is particularized.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetic modification; Public support of biotechnology; Public trust; Trust; Agribusiness; Food
Consumption/Nutrition/Food Safety.
Ano: 2006
URL: http://purl.umn.edu/62273
Bacterias antárticas y agentes antibacterianos
Provedor de dados: 20
Autores: Domínguez, M.
La Antártica se ha constituido en una región de gran interés científico debido a las condiciones extremas que allí se presentan, en
especial, de temperatura y radiación ultravioleta. En el campo de la microbiología, las investigaciones se han enfocado,
principalmente, en el estudio de la biodiversidad bacteriana, lo que ha llevado a la descripción de nuevas especies, y también
gran interés ha despertado la producción de compuestos con aplicaciones biotecnológicas. Entre éstos se encuentran los que
pudieran presentar actividad antibacteriana, de gran importancia debido a que uno de los mayores problemas de los últimos años
es la emergencia de patógenos intrahospitalarios con una amplia resistencia a los agentes antibacterianos disponibles, no
existiendo...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Biotechnology; Biotechnology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_16165;
Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2008
URL: http://hdl.handle.net/1834/3372
Poliploidização em ápices caulinares de bananeira e seus efeitos morfofisiológicos in vitro.
Provedor de dados: 119
Autores: COSTA, F.H. da S.; PASCUAL, M.; SILVA, S. de O. e; NETO, H.P. da S.; AMORIM, E.P; SANTOS-SEREJO, J.A
dos.
O objetivo deste trabalho foi avaliar as respostas morfofisiológicas de ápices caulinares de diploides de Musa acuminata
submetidos à poliploidização in vitro. Foram avaliados os antimitóticos colchicina (0, 1,25, 2,5, 3,75 e 5 mmol L-1) e orizalina (0,
15, 22,5 e 30 µmol L-1), e dois tempos de poliploidização: 24 e 48 horas para colchicina, e 3 e 7 dias para orizalina. A aplicação
de colchicina reduziu significativamente a sobrevivência e o número médio de brotos dos ápices. A orizalina teve um leve efeito
negativo na sobrevivência dos ápices caulinares e influenciou positivamente o número médio de brotos por explante. Observou-se
alta mortalidade e níveis acentuados de oxidação dos explantes primários tratados e regenerados, com ambos os antimitóticos....
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Musa acuminata; Antimitóticos; Biotecnologia; Colchicina; Morfogênese; Orizalina. antimytotic agents;
Biotechnology; Col.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/handle/doc/908061
Uso de diferentes doses do hormônio folículo estimulante suino (FSH) para induzir superovulação em
caprinos.
Provedor de dados: 119
Autores: ANDRIOLI, A.; SIMPLICIO, A. A.; FIGUEIREDO, H. O. S.; SOARES, A. T.
1994
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Caprino; Cabra; Superovulação; Embrião; Biotecnologia; Hormônio animal; Dose; Indução; Goats;
Superovulation; Embryo transfer; Animal biotechnology; FSH.
Ano: 1994
Milho BT: alternativa biotecnológica para controle biológico de insetos-praga.
Provedor de dados: 119
Autores: LOGUERCIO, L.L.; CARNEIRO, N. P.; CARNEIRO, A. A.
2002
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Milho; BT; Controle biologico; Biotecnologia; Zea mays; Biotechnology; Biological control; Lagarta do
cartucho; Spodoptera frugiperda.
Ano: 2002
Genética molecular de caracteres cuantitativos en cruzamientos dialélicos de tomate
Provedor de dados: 119
Autores: PRATTA, G.R.; MAHUAD, S.L.; DÍAZ, A.; BASTÉ, E.M.; LIBERATTI, D.R.; RODRÍGUEZ, G.R.; ZORZOLI, R.
El objetivo de este trabajo fue evaluar marcadores moleculares y caracteres cuantitativos en un cruzamiento dialélico completo sin
recíprocos, entre cinco líneas recombinantes de tomate y sus híbridos. Se obtuvieron perfiles de AFLP ("amplified fragment
length polymorphism") y de polipéptidos del pericarpio en cuatro estados de madurez del fruto de 15 genotipos. Se evaluaron,
entre otros: peso, acidez titulable, pH, vida poscosecha y firmeza. Se calculó el porcentaje de polimorfismo para los marcadores
moleculares y el porcentaje de variabilidad genética para los caracteres cuantitativos en el grupo de líneas recombinantes, el de
híbridos y el conjunto de genotipos. Se realizaron análisis de agrupamiento con cada nivel de variación genética. Para AFLP, el...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Lycopersicon; Biotecnología; Calidad del fruto; Mejoramiento genético vegetal; Recursos fitogenéticos.
biotechnology; Fr.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/handle/doc/908978
Anatomia e dendrometria de Mimosa artemisiana e Eucalyptus grandis.
Provedor de dados: 119
Autores: PAULA, J.E. de.
A madeira torna-se mais pesada a medida que a arvore envelhece, pois a densidade basica da parte mais interna do tronco, que foi
formada quando a arvore era mais nova, e mais baixa, quando comparada com a densidade de parte mais externa. Com este
trabalho pretendeu-se conferir a densidade e verificar se a madeira apresentava diferencas anatomica com o aumento da idade,
bem como gerar conhecimento biotecnologico seguro que sirva de subsidio ao melhor aproveitamento da madeira, especialmente
no que concerne a geracao de energia. O estudo baseou-se em quatro areas da secao circular do tronco, obtidas na altura do DAP
(a 1,30 m do solo), orientandas do centro para o alburno. As arvores estudadas tinham 24 anos de idade. A densidade basica de
Mimosa artemisiana...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Madeira; Arvore; Tronco; Idade; Biotecnologia; Energia; Wood; Tree; Stem; Age; Biotechnology; Energy.
Ano: 1995
URL: http://www.alice.cnptia.embrapa.br/handle/doc/104237
Acúmulo de matérias fresca e seca em plantas de mandioca (Manihot esculenta Crantz) BGM 340
(CM-305/5) micropropagadas sob diferentes concentrações de um fertilizante solúvel.
Provedor de dados: 119
Autores: SANTOS, E. B.; SOUZA, A. da S.; SILVEIRA, D. M. de S.; MENDES, M. I. de S.; SOUZA, K. A. de; VIDAL, Á. M.
A mandioca é uma cultura bastante difundida e importante, principalmente por ser constituinte da base alimentar de populações
carentes da América Latina, África e Ásia (OLIVEIRA et al., 2001), além de ter grande importância na indústria alimentícia, na
fabricação de amido e rações concentradas para animais, dentre outros produtos (SOUZA et al., 2008). É tradicionalmente
propagada por manivas. Entretanto, esta propagação ocasiona baixa produção por causa de dois fatores principais: o
envelhecimento fisiológico provocado pela constante multiplicação e a infestação por doenças que são transmitidas por sucessivas
gerações (SILVA et al., 2002), o que pode reduzir a qualidade do material de plantio e, consequentemente, a produção de raízes
(PIZA; PINHO, 2002)....
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Mandioca; Micropropagação; Reprodução de plantas; Biotecnologia; Cassava; Micropropagation; Plant
breeding; Agricultural biotechnology.
Ano: 2013
Tissue culture parameters in sweet orange cultivars.
Provedor de dados: 119
Autores: MENDES, A.F. da S.; CIDADE, L.C.; MANZOLI, G.N.; OTONI, W.C.; SOARES FILHO, W. dos S.; COSTA, M.G.C.
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants
with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with
alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot
development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant
in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was
significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration; Biotecnologia; Transferência
gênica; Morfogênese; Regeneração.
Ano: 2008
URL: http://www.alice.cnptia.embrapa.br/handle/doc/122156
Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation.
Provedor de dados: 119
Autores: VIEIRA, L. de J.; SANTANA, J. R. F. de; VIDAL, A. M.; ALVES, A. A. C.; SOUZA, A. da S.; LEDO, C. A. da S.;
SOUZA, F. V. D.
The aim of this work was to evaluate the effect of sucrose to reduce the in vitro growth of cassava plants using multivariate
statistical tests. Cassava conservation has a relevant role as an auxiliary strategy for preservation and genetic breeding.
Micro-cuttings of cassava accessions BGM 264, BGM 265, BGM 1037 and BGM 1282 from the Active Germplasm Bank of the
Embrapa Cassava and Fruits were tested with five different concentrations of sucrose (0, 14.6, 29.2, 43.8, 58.5 mM) and the
following variables were evaluated: plant height (cm), total number of leaves, number of senescent leaves, number of
micro-cuttings, size of callus, number of roots and plant vigor. The data were submitted to analysis of variance (ANOVA) and
multivariate analysis of variance...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Mandioca; Biotecnologia; Cultura de tecido; Recurso genético; Cassava; Biotechnology; Tissue culture;
Manihot esculenta; Genetic resources.
Ano: 2015
Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan
(L.) Millsp.].
Provedor de dados: 119
Autores: FERNANDES JUNIOR, P. I.; LIMA, A. A. de; PASSOS, S. R.; GAVA, C. A. T.; OLIVEIRA, P. J. de; RUMJANEK,
N. G.; XAVIER, G. R.
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt
and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the
majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated
at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very
diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total
of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Rizóbio; Seleção de estirpes; Fixação biológica de nitrogênio; Diversidade fenotípica; Feijão; Biological
nitrogen fixation; Biotechnology; Strain selection.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/handle/doc/950566
Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao.
Provedor de dados: 119
Autores: SILVA, T.E.R. da; CIDADE, L.C.; ALVIM, F.C.; CASCARDO, J.C. de M.; COSTA, M.G.C.
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao
L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli
growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium.
Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo
induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes,
irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis; Biotecnologia; Cacau; Troca gasosa;
Micropropagação; Morfogênese.
Ano: 2008
URL: http://www.alice.cnptia.embrapa.br/handle/doc/122235
Comparative analysis of grain proteins of maize sorghum, pearl millet, teosinte and tripsacum.
Provedor de dados: 119
Autores: PORTUGAL, R.S.; FIGUEIREDO, J. E. F.; VASCONCELOS, M. J. V.; PAIVA, E.; CARNEIRO, N. P.; SEDIYAMA,
T.; LOPES, M.A.
1999
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Sorgo; Milheto; Teosinto; Grao; Proteina; Biotecnologia; Maize; Sorghum; Millet; Teosinte; Grain;
Protein; Biotechnology.
Ano: 1999
Callus induction and plant regeneration in tropical maize (Zea mays L.) genotypes.
Provedor de dados: 119
Autores: VASCONCELOS, M. J. V.; ANTUNES, M.S.; BARBOSA, S.M.; LOPES, M.A.; CARVALHO, C.H.S.
1997
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Tropical; Regeneracao; Calos; Biotecnologia; Zea mays; Maize; Regeneration; Callus; Biotechnology.
Ano: 1997
Uso das ferramentas da biotecnologia no melhoramento genético do arroz: uma visão da Embrapa.
Provedor de dados: 119
Autores: BRESEGHELLO, F.
A situação atual da pesquisa em biotecnologia de arroz no Brasil. O cenário internacional da aplicação da biotecnologia para o
melhoramento genético do arroz. Proposta de plataforma internacional de pesquisa em diversidade genética de arroz. Necessidade
de formação de equipes multidisciplinares para o melhoramento genético de arroz. Perspectivas e conclusões.
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Arroz; Oryza sativa; Melhoramento genético vegetal; Biotecnologia; Rice; Plant breeding; Biotechnology.
Ano: 2009
URL: http://www.alice.cnptia.embrapa.br/handle/doc/428432
Evolução recente e tendências do agronegócio.
Provedor de dados: 119
Autores: CONTINI, E.; GASQUES, J. G.; LEONARDI, R. B. de A.; BASTOS, E. T.
Este trabalho faz parte de um conjunto de estudos realizados pelo Ministério da Agricultura, Pecuária e Abastecimento, sobre
projeções do agronegócio mundial e do Brasil. Como parte desse estudo, analisa a evolução recente do agronegócio e suas
tendências. A metodologia utilizada baseouse na análise do material produzido por instituições internacionais sobre o tema. A
evolução recente é abordada por meio de diversos indicadores do comportamento do agronegócio nos últimos anos. Além dos
indicadores usuais para analisar o tema, outros também foram usados, como o grau de abertura do agronegócio e a produtividade
total dos fatores. A partir desses indicadoes, fica claro o grau de dinamismo desse setor no Brasil. As tendências representam a
forma de identificar...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Tendência; Evolução; Agronégocio; Produtividade; Grão; Carne; Biotecnologia; Productivity; Agribusiness;
Grain; Meat; Biotechnology.
Ano: 2006
URL: http://www.alice.cnptia.embrapa.br/handle/doc/119027
Utilization of eletrophoretic technique for characterization and genetic studies in maize.
Provedor de dados: 119
Autores: DURAES, F.O.M.; PAIVA, E.; MAGALHAES, P. C.; CARVALHO, M.T.V.
1993
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Eletroforese; Caracterizacao; Genetica; Biotecnologia; Zea mays; Eletrophoretic; Characterization;
Genetic; Biotechnology.
Ano: 1993
Seleção de progênies de milho doce de alto valor nutritivo através da técnica eletroforéticas.
Provedor de dados: 119
Autores: PAIVA, E.; PARENTONI, S. N.; RODRIGUES, W.A.; PEIXOTO, M.J.V.V.D.; GAMA, E.E.G.e.
1988
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Milho doce; Melhoramento; Zea mays; Biotecnologia; Eleforese; Zea mays; Sweet corn; Breeding;
Biotechnology; Eletrophoresis.
Ano: 1988
Searching RFLP markers to identify genes for aluminium tolerance in maize.
Provedor de dados: 119
Autores: TORRES, G.A.; LOPES, M.A.; PARENTONI, S. N.; PAIVA, E.
1995
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Tolerancia; Aluminio; Biotecnologia; Zea mays; Maize; Corn; Biotechnology; Aluminum tolerance.
Ano: 1995
Effect of dicamba and AgNO3 on friable calli prodution in maize (Zea mays).
Provedor de dados: 119
Autores: CARVALHO, C. H. S.; BORDALO, P. N.; ABREU, N. L.; PAIVA, E.
1993
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Biotecnologia; Biologia molecular; Producao; Zea mays; Maize; Biotechnology; Production; Dicamba.
Ano: 1993
Identificação de híbridos de citros resistentes à mancha-marrom-de-alternária por meio de fAFLP e
testes de patogenicidade
Provedor de dados: 119
Autores: CHAGAS, E.A.; CAZETTA, J.O.; LEMOS, E.G.M.; PASQUAL, M.; GOES, A. de; RAMOS, J.D.; PIO, R.;
BARBOSA, W.; MENDONÇA, V.; AMBROSIO, L.A.
O objetivo deste trabalho foi identificar híbridos, oriundos de hibridações controladas entre 'Folha Murcha' x 'Ponkan' e testá-los
quanto à resistência a Alternaria alternata f. sp. citri. As plântulas foram obtidas via cultura in vitro de embriões. Utilizou-se o
marcador molecular fAFLP para identificação dos híbridos e, em seguida, realizou-se o teste de patogenicidade nos híbridos com
isolados de Alternaria alternata f. sp. citri, em condições de laboratório. Os pares de primers EcoRI AAG - MseI CAG e EcoRI
ACC - MseI CAA foram os mais eficientes na identificação dos híbridos, os quais identificaram 48,5% de híbridos. Os híbridos
F64, F108, F111, F113, F131 e F139 são potencialmente resistentes a Alternaria alternata f. sp. citri.
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Citrus sinensis; Citrus reticulata; Melhoramento genético; Biotecnologia vegetal; Marcador molecular; Genetic
improvement; Plant biotechnology; Molecular markers.
Ano: 2007
URL: http://www.alice.cnptia.embrapa.br/handle/doc/122770
Effect of ABA and GA3 during germination of angico vermelho [Anadenanthera peregrina (Benth.)
Speg.] embryos.
Provedor de dados: 119
Autores: BARDUCHE, D.; PAIVA, R.; PAIVA, E.; LOPES, M.A.
1995
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Biotecnologia; Semente; Angico; Cultura de tecido; Anadenanthera peregina; Biotechnology; Seed; Tissue
culture.
Ano: 1995
Cloning of putative seed storage protein genes of pearl millet (Pennisetum glaucum).
Provedor de dados: 119
Autores: CARNEIRO, N. P.; PAIVA, E.; GANDER, E.S.; MARCELLINO, L.H.; PORTUGAL, R.S.; LOPES, M.A.
2000
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milheto; Clonagem; Semente; Proteina; Armazenamento; Biotecnologia; Pennisetum glaucum; Cloning; Seed;
Storage; Protein; Biotechnology.
Ano: 2000
Comparação entre os sistemas automatizado e convencional de criopreservação de sêmen bovino.
Provedor de dados: 119
Autores: ABUD, C. O. G.; ABUD, L. J.; OLIVEIRA NETO, J. C.; DODE, M. A. N.; SERENO, J. R. B.; MARTINS, C. F.
O objetivo deste estudo foi comparar a eficiência do sistema automatizado (curva de resfriamento controlada eletronicamente) de
congelação de sêmen bovino versus o sistema convencional (curva não controlada) por meio dos parâmetros de qualidade e
viabilidade espermática no período pós-descongelação. O sêmen de quatro touros azebuados adultos foram criopreservados
simultaneamente em meio tris, gema e glicerol 7%. A avaliação computadorizada do sêmen descongelado detectou os seguintes
parâmetros:
MP
56,50±22,25%;
VAP
34,77±4,25μm/s;
VSL
28,17±4,25
μm/s;
VCL
58,45±6,85μm/s; STR 82,00±2,31%; LIN 49,50±3,32%, para o sistema automatizado e MP. 57,00±13,11%; VAP
25,75±1,66μm/s; VSL 23,32±1,99μm/s; VCL...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Curvas de congelação; Bovino; Inseminação artificial; Criopreservação; Biotecnologia; Sêmen; Cattle; Artificial
insemination; Cryopreservation; Biotechnology; Semen.
Ano: 2014
Concentrações de reguladores de crescimento vegetais no desenvolvimento in vitro do híbrido
LCREEL x (TR x LCR) 001.
Provedor de dados: 119
Autores: RABÊLO, J. S. S.; SOUZA, A. da S.; SOARES FILHO, W. dos S.; SANTOS, E. B.
A cultura de tecidos vegetais é composta por várias técnicas que apresentam alto potencial de aplicação no melhoramento
genético, além de apoiar a realização de estudos de transformação genética e conservação de espécies vegetais.
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Fruta cítrica; Cultura de tecido; Biotecnologia; Micropropagação; Citrus; Tissue culture; Biotechnology;
Micropropagation.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006033
Diagnosis maize diseases with propretary biotechnology applications transfered from Pioneer Hi-bred
International to Brazil and Latin America.
Provedor de dados: 119
Autores: KRATTIGER, A.; KULISEK, E.S.; CASELA, C.R.
1998
Tipo: Capítulo em livro técnico-científico (ALICE)
Palavras-chave: Milho; Doenca; Biotecnologia; Brasil; America Latina; Zea mays; Maize; Brazil; Latin American; Disease;
Biotechnology.
Ano: 1998
Estabilidade genética da tolerância à deterioração fisiológica pós-colheita em raízes de mandioca.
Provedor de dados: 119
Autores: VENTURINI, M. T.; SANTOS, L. R. dos; VILDOSO, C. I. A.; SANTOS, V. da S.; OLIVEIRA, E. J. de.
O objetivo deste estudo foi identificar fontes de tolerância à deterioração fisiológica pós-colheita (DFPC) da raiz da mandioca
com maior estabilidade genética nos diferentes anos de avaliação. Foram utilizadas raízes de mandioca de 418 genótipos para
avaliação DFPC em quatro ensaios. Cinco raízes de cada genótipo foram avaliadas nas porções proximal, mediana e distal da raiz
aos 0, 2, 5 e 10 dias após a colheita. Os valores genéticos preditos (?+g), bem como a estabilidade (Si), adaptabilidade (Ai) e
estabilidade + adaptabilidade (Zi) foram avaliados por meio da média harmônica do valor genotípico, desempenho relativo dos
valores genotípicos e média harmônica do desempenho relativo do valor genotípico, respectivamente, utilizando a metodologia de
modelos...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Mandioca; Pós-colheita; Biotecnologia; Cassava; Manihot esculenta; Postharvest systems; Biotechnology.
Ano: 2015
Callus induction and plant regeneration in tropical maize (Zea mays L.) genotypes for
transformation.
Provedor de dados: 119
Autores: VASCONCELOS, M. J. V.; ANTUNES, M.S.; BARBOSA, S.M.; LOPES, M.A.; CARVALHO, C.H.S.
1998
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Milho; Regeneracao; Tropical; Calo; Biotecnologia; Zea mays; Maize; Callus; Regeneration; Biotechnology.
Ano: 1998
Characterization by PCR analysis of cryl genes from Bacillus Thuringiensis strains efficient against
fall armyworm, Spodoptera frugiperda.
Provedor de dados: 119
Autores: VALICENTE, F. H.; PAIVA, E.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J.E.F.de; SANCHEZ, J.; BRAVO, A.
1997
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Lagarta do cartucho; CRY1; Controle biologico; Biotecnologia; Spodoptera frugiperda; Biotechnology;
Biological control.
Ano: 1997
Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative
model plant
Provedor de dados: 39
Autores: Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular
medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant
is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by
hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful
agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues
present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological
approaches...
Tipo: Journal article
Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013
Performance of beef cattle bulls in feed lots and fed on diets containing enzymatic complex
Provedor de dados: 2
Autores: Oliveira, Leonardo Guimarães de; Ferreira, Reginaldo Nassar; Padua, João Teodoro; Ulhoa, Cirano José; Cysneiros,
Cristine dos Santos Settimi; Arnhold, Emmanuel.
Current paper evaluates the performance of confined beef cattle supplemented with amylolytic enzyme complex produced by
fungus Aspergillus awamori and a commercial product containing multienzyme complex, yeast and MOS. Treatments comprised
control (basal diet composed of 16% Mombasa grass silage, 66% ground corn, 3% vitamin nuclear mineral and 15% cottonseed
meal), amylase treatment (control diet with the addition of 48.7 saccharifying units kg-1 diet) and compound treatment (control
diet with the addition of enzymatic complex composed of 83.2 saccharifying units, 8.8 fibrolytic units, 0.05 g of mannan
oligosaccharides and 0.2 g of inactivated yeast Kg-1 of the dry matter diet). The addition of products did not significantly increase
daily weight gain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.03.02-8 starch; Biotechnology; Feedlot; Ruminant.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/26446
Permeabilization of Saccharomyces fragilis IZ 275 cells with ethanol to obtain a biocatalyst with
lactose hydrolysis capacity
Provedor de dados: 3
Autores: Morioka, Luiz Rodrigo Ito; Colognesi, Geyci de Oliveira; Suguimoto, Hélio Hiroshi.
The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. The Saccharomyces
fragilis IZ 275 yeast cells were permeabilized with ethanol, as permeabilizing agent. To optimize the permeabilization conditions
were used the design of Box-Behnken 15 trials (3 central points). The independent variables and their levels were ethanol (29, 32
and 35%), temperature (15, 20 and 25°C) and time (15, 20 and 25 min). The answer (Y) function has beta-galactosidase activity
(U mg-1). The optimum conditions for obtaining a high enzymatic activity were observed in 35% ethanol concentration,
temperature 15ºC and 20 min. treatment time. The maximum activity of the enzyme beta-galactosidase obtained was 10.59 U
mg-1. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciência dos alimentos permeabilizing agent; Beta-galactosidase; Microbial biotechnology; Optimal conditions
Biotecnologia.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/29220
Hongos comestibles silvestres en una Zona Otomí y dinámica de colonización ectomicorrízica entre
Pinus pseudostrobus y Hebeloma mesophaeum.
Provedor de dados: 32
Autores: Heredia Acuña, Cristina.
Dentro de los ecosistemas forestales existe una amplia diversidad de microorganismos que participan en el establecimiento del
equilibrio del sistema, dentro de los cuales se encuentran de manera relevante, los hongos ectomicorrízicos. Los hongos
ectomicorrízicos forman asociaciones simbióticas con especies forestales, en esta simbiosis existe un intercambio de nutrimentos
esenciales para la vida de ambos organismos. Desde el punto de vista antropocéntrico existen hongos ectomicorrízicos que han
sido utilizados desde épocas prehispánicas como alimento o medicina, en la actualidad existe un gran interés por validar y ampliar
dichos conocimientos desde una perspectiva científica. Diversas especies han sido estudiadas con la finalidad de evaluar su
potencial...
Palavras-chave: Etnomicología; Biotecnología; Ectomicorríza; Hebeloma mesophaeum; Pinus ayacahuite; Pinos mexicanos;
Ethnomycology; Biotechnology; Ectomycorrhiza; Mexican pines; Maestría; Edafología.
Ano: 2011
URL: http://hdl.handle.net/10521/495
Hongos comestibles silvestres en una Zona Otomí y dinámica de colonización ectomicorrízica entre
Pinus pseudostrobus y Hebeloma mesophaeum.
Provedor de dados: 32
Autores: Heredia Acuña, Cristina.
Dentro de los ecosistemas forestales existe una amplia diversidad de microorganismos que participan en el establecimiento del
equilibrio del sistema, dentro de los cuales se encuentran de manera relevante, los hongos ectomicorrízicos. Los hongos
ectomicorrízicos forman asociaciones simbióticas con especies forestales, en esta simbiosis existe un intercambio de nutrimentos
esenciales para la vida de ambos organismos. Desde el punto de vista antropocéntrico existen hongos ectomicorrízicos que han
sido utilizados desde épocas prehispánicas como alimento o medicina, en la actualidad existe un gran interés por validar y ampliar
dichos conocimientos desde una perspectiva científica. Diversas especies han sido estudiadas con la finalidad de evaluar su
potencial...
Palavras-chave: Etnomicología; Biotecnología; Ectomicorríza; Hebeloma mesophaeum; Pinus ayacahuite; Pinos mexicanos;
Ethnomycology; Biotechnology; Ectomycorrhiza; Mexican pines; Maestría; Edafología.
Ano: 2011
URL: http://hdl.handle.net/10521/495
Comments on "Genetically Modified Crops: Risks and Promise" by Gordon Conway
Provedor de dados: 7
Autores: Gadgil, Madhav; Indian Institute of Science; [email protected].
Tipo: Peer-Reviewed Reports
Palavras-chave: Citizen science; Exclusion; Global development; Green revolution; New culture; Plant biotechnology; Poverty;
Public participation.
Ano: 2000
Genetically Modified Crops and the Agroecosystem: Comments on "Genetically Modified Crops:
Risks and Promise" by Gordon Conway
Provedor de dados: 7
Autores: Pimentel, David; Cornell University; [email protected].
Tipo: Peer-Reviewed Reports
Palavras-chave: Bacillus thuringiensis (B.t.); GM crops; Agriculture; Biotechnology; Economics; Ecosystems; Genetic
engineering; Herbicides.
Ano: 2000
The Elephant that is Biotechnology: Comments on "Genetically Modified Crops: Risks and Promise"
by Gordon Conway
Provedor de dados: 7
Autores: Ellstrand, Norman; University of California, Riverside; [email protected].
Tipo: Peer-Reviewed Reports
Palavras-chave: Ethics; Genetically engineered crops; Plant biotechnology; Specialists vs. generalists; Transgene.
Ano: 2000
The Risks and Benefits of Genetically Modified Crops: A Multidisciplinary Perspective
Provedor de dados: 7
Autores: Peterson, Garry D; McGill University; [email protected]; Cunningham, Saul; CSIRO Entomology;
[email protected]; Deutsch, Lisa; Department of Systems Ecology, Stockholm University;
[email protected]; Erickson, Jon; Rensselaer Polytechnic Institute; [email protected]; Quinlan, Allyson; Conservation
Ecology; [email protected]; Tinch, Robert; School of Environmental Sciences, University of East Anglia;
[email protected]; Troell, Max; Beijer Institute, Royal Swedish Academy of Sciences; [email protected]; Woodbury,
Peter; Boyce Thompson Institute for Plant Research; [email protected]; Zens, Scot; Department of Biology, Dartmouth College;
[email protected].
The benefits and risks of any particular GM crop depend on the interactions of its ecological functions and natural history with the
agroecosystem and ecosystems within which it is embedded. These evolutionary and ecological factors must be considered when
assessing GM crops. We argue that the assessment of GM crops should be broadened to include alternative agricultural practices,
ecosystem management, and agricultural policy. Such an assessment would be facilitated by a clearer understanding of the
indirect costs of agriculture and the ecological services that support it. The benefits of GM crops should be compared to those of
other means of agricultural intensification such as organic farming, integrated pest management, and agricultural policy reform....
Tipo: Peer-Reviewed Reports
Palavras-chave: Agriculture; Biotechnology; Genetically modified crops (GM); Interdisciplinary; Public dialogue; Regulation;
Risk assessment.
Ano: 2000
Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects
Provedor de dados: 69
Autores: Jube,Sandro; Borthakur,Dulal.
Tobacco is the most commonly used plant for expression of transgenes from a variety of organisms, because it is easily grown and
transformed, it provides abundant amounts of fresh tissue and has a well-established cell culture system. Many bacterial proteins
involved in the synthesis of commercial products are currently engineered for production in tobacco. Bacterial enzymes
synthesized in tobacco can enhance protection against abiotic stresses and diseases, and provide a system to test applied strategies
such as phytoremediation. Examples of bacterial gene expression in tobacco include production of antigen proteins from several
human bacterial pathogens as vaccines, bacterial proteins for enhancing resistance against insects, pathogens and herbicides,...
Tipo: Journal article
Palavras-chave: Biotechnology; Environmental stress; Insect/pathogen/herbicide resistance; Phytoremediation; Transgenics;
Vaccine production.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300013
Using Bcl-xL anti-apoptotic protein for altering target cell apoptosis
Provedor de dados: 69
Autores: Iz,Sultan Gülçe; Çalimlioglu,Beste; Gürhan,Saime Ismet Deliloglu.
Background: Altering target cell apoptosis is one of the challenging ideas of biotechnological applications. There are several
applications of over expressing Bcl-xL anti-apoptotic protein from recombinant protein production to DNA vaccination strategies.
The aim of the present study is to evaluate the anti-apoptotic efficacy of Bcl-xL expressing dual promoter plasmid system as a
candidate to be used for recombinant protein production and DNA vaccination approaches. For this purpose, Bcl-xL anti-apoptotic
protein gene was inserted in a dual expressing vector system in frame with EGFP (enhanced green fluorescence protein) after
IRES (internal ribosomal site). The plasmid has a multiple cloning site after CMV (cytomegalovirus promoter) left empty to be...
Tipo: Journal article
Palavras-chave: Animal cell biotechnology; Apoptosis; Bcl-xL anti-apoptotic protein.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500002
An economic valuation of a biotechnology R&D project in a developing economy
Provedor de dados: 69
Autores: Erbas,Bahar Celikkol; Memis,Selin Arslanhan.
Background: Biotechnology complements technological developments in main sectors of economies, such as health, energy, and
agriculture, and thus contributes to economic development. It provides solutions to the problems that are frequently faced in
developing economies, such as resource constraints, lower productivity and environmental concerns. In order to benefit from
biotechnology, its associated markets need to develop and function well to support the developments and transactions of
intangible assets, such as technology transfers, license agreements and research and development joint ventures. Economic
valuation of the intangible assets is necessary for the development and functioning of these markets. It provides better
understanding of value...
Tipo: Journal article
Palavras-chave: Biotechnology sector; Developing economies; Real options; R&D project valuation; Turkey.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300001
Eutils-search - manual do usuário.
Provedor de dados: 14
Autores: HIGA, R. H.; YASUDA, R. S.
O software Eutils-search tem por objetivo trazer do banco de dados PubMed informações sobre artigos relacionados a genes de
um organismo específico, de acordo com as regras referentes à taxa de acesso impostas pelo site. As informações trazidas são,
então, armazenadas localmente em um banco de dados para acesso rápido. Além disso, o software também gera documentos XML
correspondentes às informações do organismo requisitado. O eutils-search é uma ferramenta de apoio ao desenvolvimento de
aplicações de mineração de textos voltadas para os domínios de biotecnologia e biologia molecular, baseada em informações
textuais obtidas do banco de dados PubMed. Este documento apresenta os pré-requisitos e a descrição dos parâmetros necessários
para utilização do...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software eutils-search; Biotecnologia; Biologia molecular; Mineração de textos; Software; Biotechnology;
Information retrieval.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/883643
Variabilidade genética de cultivares de coqueiro anão e híbridos com base em marcadores
moleculares RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; VARGAS RAMOS, V. H.; BELLON, G.; JUNQUEIRA, K. P.; AZEVEDO, J. A.; ARAGÃO, W. M.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; Variação genética; Biotecnologia; Coco; Cocos nucifera; Genetic markers; Genetic
variation; Biotechnology; Coconuts.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570177
Distancia do fluxo genico ocorrido entre a cultivar de soja BR-16 transgenica e BR-16 nao-transgenica
no Cerrado do Distrito Federal.
Provedor de dados: 14
Autores: ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; VIANNA, G. R.; ANDRADE, S. R. M.; RECH, E. L.; ARAGAO, F. J.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Biotecnologia; Transgenico; Soja; Glycine max; Melhoramento genetico; Genetica vegetal; Biotechnology;
Transgenics; Soybeans; Plant breeding; Genetics.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567427
Variabilidade genética de acessos de mandioca morfologicamente similares à etnovariedade 'buriti'
com base em marcadores moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FIALHO, J. de F.; BELLON, G.; VIEIRA, E. A.; FUKUDA, W. M. G.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador genético; Biotecnologia; Marcador molecular; Mandioca; Manihot esculenta;
Genetic variation; Genetic markers; Cassava; Biotechnology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570172
Variabilidade genética de acessos de mandioca similares às variedades 'Pioneira' e 'Japonesa'
mantidos em banco de germoplasma e cultivados em núcleos rurais com base em marcadores
moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FIALHO, J. de F.; BELLON, G.; VIEIRA, E. A.; FUKUDA, W. M. G.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador genético; Biotecnologia; Marcador molecular; Mandioca; Manihot esculenta;
Genetic variation; Genetic markers; Cassava; Biotechnology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570174
Avaliacao do fluxo genico entre a cultivar de soja BR-16 transgenica e BR-16 nao-transgenica no
ecossistema do Cerrado no Distrito Federal.
Provedor de dados: 14
Autores: ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; VIANNA, G. R.; ANDRADE, S. R. M.; RECH, E. L.; ARAGAO, F. J.
L.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Melhoramento genetico vegetal; Soja; Glycine max; Biotecnologia; Planta transgenica; Plant breeding;
Soybeans; Biotechnology; Transgenic plants.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567487
Análise da variabilidade genética molecular de uma coleção de trabalho de guandu (Cajanus cajan)
como apoio na seleção de acessos para testes agronômicos em sistemas de integração lavoura-pecuária
e recuperação de pastagens degradadas.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FERNANDES, F. D.; BARCELLOS, A. O.; ANDRADE, R. P.; BELLON, G.; JUNQUEIRA, K. P.;
AMABILE, R. F.; MARTHA JÚNIOR, G. B.; VILELA, L.; RAMOS, A. K. B.; KARIA, C. T.; GODOY, R.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador molecular; Marcador genético; Biotecnologia; Leguminosa forrageira; Genetic
variation; Genetic markers; Biotechnology; Feed legumes.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570173
Biotecnologia de produção massal e manejo de Trichogramma para o controle biológico de pragas
Provedor de dados: 14
Autores: ALMEIDA, R. P. de; SILVA, C. A. D. da; MEDEIROS, M. B. de.
1998
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Trichogramma; Manejo; Producao; Biotecnologia; Controle biologico; Biological control; Production;
Biotechnology; Pests; Management.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/362591
Isolamento, cultivo criopreservação de células somáticas de mamíferos silvestres para formação de
um banco de germoplasma.
Provedor de dados: 14
Autores: MARTINS, C. F.; CUMPA, H. C. B.; CUNHA, E. R. da; SILVA, C. G. da; BORGES, L. A.; GAMA FILHO, J. B. da.
2013
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Animal silvestre; Germoplasma; Mamífero; Criopreservação; Animal conservation; Biotechnology;
Reproduction; Wild animals; Genebank; Cryopreservation.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/981278
Laboratório de biotecnologia de plantas.
Provedor de dados: 14
Autores: EMBRAPA AMAZÔNIA ORIENTAL.
2001
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Instituicao de pesquisa; Pesquisa agricola; Planta; Laboratorio; Belem; Pará; Amazonia; Brasil; Research;
Plants; Biotechnology.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/373934
Melhoramento da manga (Mangifera indica L.) no Brasil: sinopse de projeto.
Provedor de dados: 14
Autores: PINTO, A. C. de Q.; ANJOS, J. R. N. dos; ANDRADE, S. R. M.; FALEIRO, F. G.; CORDEIRO, M. C. R.; DIAS, J. N.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Melhoramento genetico; Manga; Mangifera indica; Fruta tropical; Biotecnologia; Plant breeding; Mango;
Tropical fruits; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567612
Eutils-search versão 2.0 - manual do usuário.
Provedor de dados: 14
Autores: TANAKA, R. S.; HIGA, R. H.
O software Eutils-search tem por objetivo trazer do banco de dados PubMed informações sobre artigos relacionados a genes de
um organismo específico, de acordo com as regras referentes à taxa de acesso impostas pelo site. As informações trazidas são,
então, armazenadas localmente em um banco de dados para acesso rápido. Além disso, o software também gera documentos XML
correspondentes às informações do organismo requisitado. O eutils-search é uma ferramenta de apoio ao desenvolvimento de
aplicações de mineração de textos voltadas para os domínios de biotecnologia e biologia molecular, baseada em informações
textuais obtidas do banco de dados PubMed. Este documento apresenta os pré-requisitos e a descrição dos parâmetros necessários
para utilização do...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software eutils-search; Biotecnologia; Biologia molecular; Mineração de texto; Text mining; Software;
Biotechnology; Molecular biology.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/920856
Histórico da Comissão Interna de Biossegurança da Embrapa Cerrados.
Provedor de dados: 14
Autores: FALEIRO, F. G.; ANDRADE, S. R. M. de; SOUZA, P. I. de M.; MOREIRA, C. T.; ABUD, S.; MENDES, I. de C.;
REIS JÚNIOR, F. B. dos.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Biotecnologia; Transgênico; Genética vegetal; Melhoramento genético vegetal; Biotechnology; Transgenics;
Genetics; Plant breeding.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/569168
Aplicação de descritores morfoagronômicos utilizados em ensaios de DHE de cultivares de
maracujazeiro-doce, ornamental, medicinal, incluindo espécies silvestres e híbridos interespecíficos
(Passiflora spp.): manual prático.
Provedor de dados: 14
Autores: JESUS, O. N. de; OLIVEIRA, E. J. de; SOARES, T.L.; FALEIRO, F. G.
2015
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Maracujá-doce; Passiflora spp; Cultivar; Melhoramento genético; Biotecnologia; Passion fruit; Varieties;
Genetic improvement; Biotechnology.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/1048464
Aplicação de descritores morfoagronômicos utilizados em ensaios de DHE de cultivares de
maracujazeiro-azedo (Passiflora edulis Sims): manual prático.
Provedor de dados: 14
Autores: JESUS, O. N. de; OLIVEIRA, E. J. de; SOARES, T.L.; FALEIRO, F. G.
2015
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Maracujá-azedo; Passiflora edulis; Cultivar; Melhoramento genético; Biotecnologia; Passion fruit; Varieties;
Genetic improvement; Biotechnology.
Ano: 2015
Producing recombinant commercial proteins
Provedor de dados: 5
Autores: Cadoret, Jean-paul; Bardor, M; Lerouge, P; Cabigliera, M; Henriquez, Vitalia; Carlier, Aude.
Extraction of natural substances and chemical synthesis are the main sources of pharmaceutical molecules. When possible, one
may transfer the gene of the molecule in living cells creating individual factories producing on demand and in a safe way the
requested molecule. Today, bacteria, yeast, mammalian cells and plants constitute the main plate-forms for various commercial
products. Microalgae present numerous advantages and could offer a powerful tool for the production of commercial molecules in
a near future.
Tipo: Text
Palavras-chave: Genetics; Recombinant; Algae; Pharmaceuticals; Gene transfer; Biotechnology; Génétique; Recombinant;
Algues; Pharmaceutique; Transfert de gène; Biotechnologie.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4045.pdf
Unique for human centromeric regions of interphase chromatin homing (CENTRICH) govern
dynamic features of chromatin fractal globules
Provedor de dados: 17
Autores: Gennadi Glinsky.
We report the results of the genome-wide alignment of inter- and intra-chromosomal chromatin interactions within the context of
interphase chromatin binding to nuclear lamina and nucleolus. For all human chromosomes, a significant correlation exists
between binding of chromosomal loci to nuclear lamina and segregation into spatially-defined distinct compartments of
genome-wide chromatin interactions identified by Hi-C method. We report identification of near-centromeric intergenic regions
on human chromosomes (chr2; chr10; chr17; chr1), which are highly enriched for interphase chromatin homing sites and function
as attractors of long-range physical interactions (Centromeric Regions of Interphase Chromatin Homing, CENTRICH).
CENTRICH are engaged in 397-1526...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6756/version/1
Marked Rise in Replikin Counts in H5N1 Influenza Virus Localized to Lethality Gene p B1.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Abstract: Virus outbreaks have been found to be related to the concentration of a new class of genomic peptides, Replikins^1^.
The eight genes of H5N1 influenza virus were analyzed for the distribution of Replikin Counts (number Replikins /100 amino
acids) in 2,441 sequences from birds and humans. An increase (p<0.001) occurred from 2004 to August 2011 in one
gene, pB1.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Microbiology; Molecular Cell Biology;
Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6420/version/1
Synchronized Mammalian Cells in Culture: A Comparison of Normal and KB Cancer Cell Growth
Provedor de dados: 17
Autores: I. C. Baianu.
Experimentation and tests with cells in culture and cell synchronization or synchrony are important in order to be able to make
both reliable and high-sensitivity measurements. This is also very important for mechanistic studies of cell division control,
oncogenesis and tumor growth. A comparison is made in this article between the initially synchronized cell growth of rabbit and
KB cells in synchronized cultures.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7128/version/1
Nonlinear Models of Neural and Genetic Network Dynamics
Provedor de dados: 17
Autores: I. C. Baianu.
A categorical and Łukasiewicz-Topos framework for Łukasiewicz LM-Algebraic Logic models of
nonlinear dynamics in complex functional systems such as neural networks, genomes and cell interactomes is proposed.
Łukasiewicz Algebraic Logic models of genetic networks and signaling pathways in cells are formulated in terms of
nonlinear dynamic systems with n-state components that allow for the generalization of previous logical models of both genetic
activities and neural networks.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7114/version/1
Cancer Clinical Trials Optimization and Pharmacogenomics
Provedor de dados: 17
Autores: I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view
to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer
trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell
genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is
rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and
computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology;
Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7046/version/1
The 'consciousness' of the living state: Extending the Baars global broadcast
model across physiological subsystems
Provedor de dados: 17
Autores: Rodrick Wallace.
For the broad spectrum of cognitive biological phenomena having 'dual' information sources, isolation
from crosstalk between them requires more metabolic free energy than permitting correlation. This allows an evolutionary
exaptation leading to dynamic global broadcasts at multiple scales, similar to the well-studied exaptation of noise to trigger
stochastic resonance amplification in physiological systems. Entropy gradient models adapted from nonequilibrium
thermodynamics lead to an index theorem in which analytic solutions of empirical equations describe different possible
topological modes. Not only is the living state characterized by cognition at every scale and level of organization, but by multiple,
shifting, tunable, cooperative...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Immunology; Molecular Cell Biology; Neuroscience;
Pharmacology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6898/version/2
Early Diagnosis of Alzheimer's disease by NIRF Spectroscopy and Nuclear
Medicine-v.4.0
Provedor de dados: 17
Autores: I Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer’s (AD) and Cancers in order to enable
their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death
with major, human and financial/economics trillion dollar consequences for the society. Nuclear Medicine is concerned with
applications in Medicine of Nuclear Science and Engineering techniques and knowledge. Three major Nuclear Medicine
techniques that are established for diagnostic and research purposes are: Positron Emission Tomography (PET) and CAT/CT,
Nuclear Magnetic Resonance Imaging (NMRI/MRI). However, these three techniques have also major limitations in terms of
either cost or image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology;
Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6273/version/1
Food Physical Chemistry and Biophysical Chemistry
Provedor de dados: 17
Autores: I.C. Baianu.
Food Physical Chemistry is considered to be a branch of Food Chemistry^1,2^ concerned with the study of both physical and
chemical interactions in foods in terms of physical and chemical principles applied to food systems, as well as the applications of
physical/chemical techniques and instrumentation for the study of foods^3,4,5,6^.
This field encompasses the
"physiochemical principles of the reactions and conversions that occur during the manufacture, handling, and storage
of foods"^7^. Two rapidly growing, related areas are Food Biotechnology and Food Biophysical Chemistry.


Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Microbiology; Molecular Cell Biology; Pharmacology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6573/version/1
The Differences Between Products Of Gene Expression In Male, Female And Hermaphrodite
Cucumber Floral Buds (Cucumis Sativus L.)
Provedor de dados: 17
Autores: Magdalena Pawelkowicz; Ewa Siedlecka; Zbigniew Przybecki.
Cucumber is a species in which sex expression has been extensively studied. Sexual differentiation is controlled by genotypic and
environmental factors. The main genes responsible for sex determination have been described but the mechanism of their action
remains unexplained. In this study we attempted to find cDNA clones which can be connected with sex differentiation and flower
development
in cucumber. 
Two pairs of nearly isogenic lines: GY-3 (gynoecious –
FFMMGG) and HGY-3 (hermaphrodite - FFmmGG), B10 (monoecious - ffMMGG) and 2gg (gynoecious - FFMMgg) were used
to search for differences in gene expression in young (1 – 2mm) cucumber floral buds.
In
order to obtain differentially expressed cDNA...
Tipo: Poster
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5605/version/1
Early Diagnosis of Alzheimer's disease by NIRF Spectroscopy and Nuclear Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer’s (AD) and Cancers in order to enable
their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death
with major, human and financial/economics trillion dollar consequences for the society. Nuclear Medicine is concerned with
applications in Medicine of Nuclear Science and Engineering techniques and knowledge. Three major Nuclear Medicine
techniques that are established for diagnostic and research purposes are: Positron Emission Tomography (PET) and CAT/CT,
Nuclear Magnetic Resonance Imaging (NMRI/MRI). However, these three techniques have also major limitations in terms of
either cost or image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Molecular Cell Biology; Neuroscience;
Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6271/version/1
Quantitative Models for Efficient Cloning of Different Vectors with Various Clone sites
Provedor de dados: 17
Autores: Gang Zhang; Anurag Tandon.
We developed an efficient "Combinatorial Strategy” for cloning different vectors with various clone
sites. 1) Using originally existed clone sites from circular vectors to prepare the inserts, and if no appropriate sites are available,
performing SDM to create compatible sites, to achieve maximal correct digestion of the inserts. 2) Different vectors were digested
with various restriction endonucleases, and then dephosphorylated with CIP. 3) Top10 competent cells were used for
transformation to increase the transformant colonies. Our results showed that, when blunt sites, or a Xba I site was adopted for
ligation, the percentages of positive clones were about 50%. Whereas, when different sites, including one blunt and another Pst I...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Immunology; Microbiology;
Molecular Cell Biology; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/6965/version/1
A Critical Evaluation of Clinical Trials in Cancer and Pharmacogenomics
Provedor de dados: 17
Autores: I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view
to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer
trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell
genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is
rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and
computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology;
Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7052/version/1
Narrow genetic and apparent phenetic diversity in Jatropha curcas: initial success with generating
low phorbol ester interspecific hybrids
Provedor de dados: 17
Autores: Siam Popluechai; Diego Breviario; Sujatha Mulpuri; Harinder P. S. Makkar; Manish Raorane; Attipalli R. Reddy;
Enrico Palchetti; Angharad M. R. Gatehouse; Keith J. Syers; Anthony G. O'Donnell; Ajay Kohli.
Due to the increasing popularity of Jatropha curcas as a feedstock for biodiesel, generating non-toxic and high yielding varieties of
the plant requires genotypic characterization towards identifying breeding lines. There is little information on the phylogenetic
relationships between its global accessions and species. Assessing genetic variation by RAPD, AFLP and combinatorial tubulin
based polymorphism (cTBP) in 38 J. curcas accessions from 13 countries on 3 continents revealed narrow genetic diversity.
However, 6 different species of Jatropha from India exhibited pronounced genetic diversity indicating possibilities of improving J.
curcas by interspecific breeding. The relatively unexplored cTBP approach we used was a highly efficient and cost effective...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Earth & Environment; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2782/version/1
Counting absolute number of molecules using unique molecular identifiers
Provedor de dados: 17
Autores: Teemu Kivioja; Anna Vähärautio; Kasper Karlsson; Martin Bonke; Sten Linnarsson; Jussi
Taipale.
Advances in molecular biology have made it easy to identify different DNA or RNA species and to copy them. Identification of
nucleic acid species can be accomplished by reading the DNA sequence; currently millions of molecules can be sequenced in a
single day using massively parallel sequencing. Efficient copying of DNA-molecules of arbitrary sequence was made possible by
molecular cloning, and the polymerase chain reaction. Differences in the relative abundance of a large number of different
sequences between two or more samples can in turn be measured using microarray hybridization and/or tag sequencing. However,
determining the relative abundance of two different species and/or the absolute number of molecules present in a single sample
has proven much...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5903/version/1
Food Physical Chemistry and Biophysical Chemistry (v2)
Provedor de dados: 17
Autores: I. C. Baianu.
Food Physical Chemistry is considered to be a branch of Food Chemistry^1,2^ concerned with the study of both physical and
chemical interactions in foods in terms of physical and chemical principles applied to food systems, as well as the applications of
physical/chemical techniques and instrumentation for the study of foods^3,4,5,6^.
This field encompasses the
"physiochemical principles of the reactions and conversions that occur during the manufacture, handling, and storage
of foods"^7^. Two rapidly growing, related areas are Food Biotechnology and Food Biophysical Chemistry.


Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Microbiology; Molecular Cell Biology; Pharmacology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6573/version/2
NIR Spectroscopy and Microspectroscopy Analysis of Intact Soybean Seeds for Food Applications
through Composition Improvements. Version 2.0
Provedor de dados: 17
Autores: Tiefeng You; I. C. Baianu.
The soybean-derived products are among the most important agricultural products in the USA and the world. Conventional
analytical methods for soybean composition analysis are both time consuming and costly. Faster and less expensive methods are
required for most practical applications. To improve the accuracy, reliability and sensitivity of NIR, major advancements in
instrumentation, as well as, data analysis / calibration methodology are required. Novel NIR instruments, such as DA-NIR and
FT-NIR spectrometers developed in recent years have the potential for improving significantly the quantification of soybean
composition at a reasonable cost. We present representative calibrations and data for intact soybean composition analysis obtained
at the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6253/version/1
Multi-scale network biology model & the model library
Provedor de dados: 17
Autores: Jianghui Xiong.
Multi-scale network biology model & the model library
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell
Biology; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5778/version/1
NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil,
Proteins and Water Contents [v.2]
Provedor de dados: 17
Autores: I. C. Baianu; Jun Guo; Randall L. Nelson; Tiefeng You; Doina M. Costescu.
Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared
(NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed
composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique
development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert
and Beer’s law, and quantitative measurements can be successfully made with high speed and ease of operation.
NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the
food industry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6611/version/2
Complex Systems Analysis of Arrested Neural Cell Differentiation during Development and
Analogous Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: I C. Baianu; V. I. Prisecaru.
A new approach to the modular, complex systems analysis of nonlinear dynamics of arrested neural cell Differentiation--induced
cell proliferation during organismic development and the analogous cell cycling network transformations involved in
carcinogenesis is proposed. Neural tissue arrested differentiation that induces cell proliferation during perturbed development and
Carcinogenesis are complex processes that involve dynamically inter-connected biomolecules in the intercellular, membrane,
cytosolic, nuclear and nucleolar compartments. Such 'dynamically inter-connected' biomolecules form
numerous inter-related pathways referred to as 'molecular networks'. One such family of signaling
pathways contains the cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7101/version/1
Genetic Normalization of Differentiating Aneuploid Human Embryos
Provedor de dados: 17
Autores: Paul Brezina; Andrew Barker; Andrew Benner; Ric Ross; Khanh-Ha Nguyen; Raymond Anchan; Kevin Richter; Garry
Cutting; William Kearns.
Early embryogenesis involves a series of dynamic processes, many of which are currently not well described or understood.
Aneuploidy and aneuploid mosaicism, a mixture of aneuploid and euploid cells within one embryo, in early embryonic
development are principal causes of developmental failure.^1,2^ Here we show that human embryos demonstrate a significant rate
of genetic correction of aneuploidy, or "genetic normalization" when cultured from the cleavage stage on
day 3 (Cleavage) to the blastocyst stage on day 5 (Blastocyst) using routine in vitro fertilization (IVF) laboratory conditions. One
hundred and twenty-six human Cleavage stage embryos were evaluated for clinically indicated preimplantation genetic screening
(PGS). Sixty-four of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6045/version/1
Ecological, phenological and biochemical significance of bimodal fruiting of Boletus alutaceus morgan
Provedor de dados: 17
Autores: Nandkumar Kamat.
Boletus alutaceus Morgan an ectomycorrhizal species was extensively studied from an ecologically fragmented microhabitt
colonized by its' host plant Ficus benghalensis L. in the Goa University campus, Taleigao, Goa, India. Qualitatively
the fruiting cycles were monitored for three years and thereafter detail ecological and morphological investigations were carried
out in May and November 2004. A bimodal temporal fruting pattern emerged which may have significant ecological,
phenological and biochemical role in EM dynamics.
Tipo: Presentation
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Genetics & Genomics; Microbiology; Plant Biology;
Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2835/version/1
Single Cancer Cell Detection by Near Infrared Microspectroscopy, Infrared Chemical Imaging and
Fluorescence Microspectroscopy
Provedor de dados: 17
Autores: I. C. Baianu; Schuyler S. Korban; Doina M. Costescu.
Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer
cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier Transform
Infrared (FT-IR), Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems
approaching 1micron resolution are here reported. 400 and 500 MHz, H-1 NMR analyses were carried out that allowed the
selection of mutagenized embryos. Detailed chemical analyses are being demonstrated to be also possible by FT-NIR Chemical
Imaging/ Microspectroscopy of single cancer cells. FT-NIR Microspectroscopy and Chemical Imaging are also shown to be
potentially important in Functional...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Molecular Cell Biology; Bioinformatics; Plant
Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6207/version/1
Genomic Replikin Count Predicts Increased Lethality of Malaria
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Genomic Replikin Counts predict both the increase and the decrease of lethality of malaria
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Immunology; Microbiology; Molecular Cell Biology;
Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7142/version/1
Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and
High-Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Embryos and Single Cells
Provedor de dados: 17
Autores: I. C. Baianu; Schyler S. Korban; Tiefeng You.
Chemical analysis of soybean seeds, somatic embryos and single cells were carried out by Fourier Transform Infrared (FT-IR),
Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fluorescence and High-Resolution NMR (HR-NMR). The first
FT-NIR chemical images of biological systems approaching 1 micron (1μ) resolution are presented here. Chemical
images obtained by FT-NIR and FT-IR Microspectroscopy are presented for oil in soybean seeds and somatic embryos under
physiological conditions. FT-NIR spectra of oil and proteins were obtained for volumes as small as 2μ3. Related,
HR-NMR analyses of oil contents in somatic embryos are also presented here with nanoliter precision. Such 400 MHz 1H NMR
analyses allowed the selection of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6200/version/1
Soybean Composition Database from NIR, NMR and GC-MS Analyses
Provedor de dados: 17
Autores: I. C. Baianu.
This novel Soybean Composition Database from the AFC-NMR & NIR Spectroscopy Facility of the College of ACES
at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean
Germplasm Collection, such as those received from Peking at the National Soybean Collection.
Excel files
(.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made
available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based
software. 

A detailed account is also presented of our high-resolution nuclear
magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/1
Multifunction moonlighting and intrinsically disordered proteins: Information catalysis, nonrigid
molecule symmetries, and the 'logic gate' spectrum
Provedor de dados: 17
Autores: Rodrick Wallace.
Intrinsically disordered proteins (IDP) appear far more likely to engage in functional moonlighting than well-structured proteins.
The recent use of nonrigid molecule theory to address IDP structure and dynamics produces this result directly: Mirror image
subgroup or subgroupoid tiling matching of the molecular fuzzy lock-and-key can be much richer for IDPs since the number of
possible group or groupoid symmetries can grow exponentially with molecule length, while tiling matching for 3D structured
proteins is relatively limited. A simple information catalysis model suggests how this mechanism can produce a vast spectrum of
biological 'logic gates' having subtle properties far beyond familiar AND, OR, XOR, etc. behaviors.
Inferring the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Molecular Cell Biology; Pharmacology;
Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6413/version/1
Soybean Composition Database from NIR, NMR and GC-MS Analyses
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Jun Guo; Doina M. Costescu; V. I. Prisecaru.
This novel Soybean Composition Database from the AFC-NMR & NIR Spectroscopy Facility of the College of ACES
at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean
Germplasm Collection, such as those received from Peking at the National Soybean Collection.
Excel files
(.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made
available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based
software. 

A detailed account is also presented of our high-resolution nuclear
magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/2
Systems Biology on Bio-Linux
Provedor de dados: 17
Autores: Mesude Bicak.
This presentation introduces the Bio-Linux Operating System which is a popular Computational Biology workstation with 500+
bioinformatics software. It then provides an overview of popular modelling approaches in systems biology, covering top-down,
bottom-up and hybrid modelling. Finally it highlights the challenges being faced by systems biology modellers, agent-based
modellers in particular, regarding standardisation efforts.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Molecular Cell Biology; Bioinformatics;
Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6348/version/1
Pioneer Discovery of secondary gold deposits-unique bacterioforms andplacers in north Goa from
deep coremetabiosphere
Provedor de dados: 17
Autores: Nandkumar M. Kamat.
Bacterioform secondary gold deposits were discovered in tilloid overlaying archaean base rock (metagraywacke) in Sanvordem
rock formation, at the depth of 65 metres in north Goa , India and were confirmed optically as microbiogenic Gold. Multiple and
unique forms were seen. This is claimed to be first report of bacterioform Gold in deep cores in Goa, India and Asia.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics & Genomics; Microbiology; Earth & Environment;
Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5497/version/1
Novel network pharmacology methods for drug mechanism of action identification, pre-clinical drug
screening and drug repositioning
Provedor de dados: 17
Autores: Jianghui Xiong.
The high rates of failure in oncology drug clinical trials highlight the problems of using pre-clinical data to predict the clinical
effects of drugs. Here we present two methodology innovations on network pharmacology modeling. (1) We hypothesize that the
gene network associated with cancer outcome in heterogeneous patient populations could serve as a reference for identifying drug
effects. We proposed a novel in vivo genetic interaction between genes as ‘synergistic outcome
determination’, in a similar way to ‘synthetic lethality’. We scanned above genetic
interactions based on microarray profiling for cancer prognosis, and identified a cluster of important yet epigenetically regulated
gene modules. By...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell
Biology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6455/version/1
Exploring The Hidden Realms Of Global Biodiversity: Interesting Endophytic Yeast Flora From
Floral Ovaries
Provedor de dados: 17
Autores: Nandkumar Kamat; Shweta S. Saudagar.
Soil is considered the ultimate reservoir of all the microbiota and specifically the yeasts. However their association with different
plant organs and at different developmental stages is not fully explored. Although about 1000 yeast species have been identified,
exploration of various microhabitats such as plant storage organs, flowers, nectar, and tree sap is expected to yield a large number
of novel species, including interesting endophytic yeasts(Carlos & Gabor, 2006). Angiosperms are considered the most
diverse group of the plant kingdom comprising of about 250,000 species (Kenrick, 1999). As part of an exhaustive exploration of
yeasts associated with angiosperm flowers, specific attention was paid to floral ovaries to detect the presence of...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics & Genomics; Microbiology; Plant Biology; Evolutionary
Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6268/version/1
Applications Of Microspectroscopy, Hyperspectral Chemical Imaging And Fluorescence Microscopy
In Chemistry, Biochemistry, Biotechnology, Molecular And Cell Biology
Provedor de dados: 17
Autores: I. C. Baianu.
Chemical imaging is a technique for the simultaneous measurement of spectra (chemical information) and images or pictures
(spatial information)^1,2^. The technique is most often applied to either solid or gel samples, and has applications in chemistry,
biology^3-8^, medicine^9,10^, pharmacy^11^ (see also for example: Chemical Imaging Without Dyeing), food science, Food
Physical Chemistry, Biotechnology^12,13^, Agriculture and industry. NIR, IR and Raman chemical imaging is also referred to as
hyperspectral, spectroscopic, spectral or multi-spectral imaging (also see micro-spectroscopy). However, other ultra-sensitive and
selective, chemical imaging techniques are also in use that involve either UV-visible or fluorescence microspectroscopy.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6593/version/1
Translational Oncogenomics and Human Cancer Interactome Networks
Provedor de dados: 17
Autores: I. C. Baianu.
An overview of translational, human oncogenomics, transcriptomics and cancer interactomic networks is presented together with
basic concepts and potential, new applications to Oncology and Integrative Cancer Biology. Novel translational oncogenomics
research is rapidly expanding through the application of advanced technology, research findings and computational tools/models
to both pharmaceutical and clinical problems. A self-contained presentation is adopted that covers both fundamental concepts and
the most recent biomedical, as well as clinical, applications. Sample analyses in recent clinical studies have shown that gene
expression data can be employed to distinguish between tumor types as well as to predict outcomes. Potentially important
applications...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology;
Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6190/version/2
The “Minimum Information about an ENvironmental Sequence”
(MIENS) specification
Provedor de dados: 17
Autores: Pelin Yilmaz; Renzo Kottmann; Dawn Field; Rob Knight; James R. Cole; Linda Amaral-Zettler; Jack A. Gilbert; Ilene
Karsch-Mizrachi; Anjanette Johnston; Guy Cochrane; Robert Vaughan; Christopher Hunter; Joonhong Park; Norman
Morrison; Phillipe Rocca-Serra; Peter Sterk; Mani Arumugam; Laura Baumgartner; Bruce W. Birren; Martin J. Blaser; Vivien
Bonazzi; Peer Bork; Pier Luigi Buttigieg; Patrick Chain; Elizabeth K. Costello; Heather Huot-Creasy; Peter Dawyndt; Todd
DeSantis; Noah Fierer; Jed Fuhrman; Rachel E. Gallery; Richard A. Gibbs; Michelle Gwinn Giglio; Inigo San Gil; Elizabeth M.
Glass; Antonio Gonzalez; Jeffrey I. Gordon; Robert Guralnick; Wolfgang Hankeln; Sarah Highlander; Philip Hugenholtz; Janet
Jansson; Jerry Kennedy; Dan Knights; Omry Koren; Justin Kuczynski; Nikos Kyrpides; Robert Larsen; Christian L. Lauber;
Teresa Legg; Ruth E. Ley; Catherine A. Lozupone; Wolfgang Ludwig; Donna Lyons; Eamonn Maguire; Barbara A.
Methé; Folker Meyer; Sara Nakielny; Karen E. Nelson; Diana Nemergut; Josh D. Neufeld; Norman R. Pace;
Giriprakash Palanisamy; Jörg Peplies; Jane Peterson; Joseph Petrosino; Lita Proctor; Jeroen Raes; Sujeevan
Ratnasingham; Jacques Ravel; David A. Relman; Susanna Assunta-Sansone; Lynn Schriml; Erica Sodergren; Aymé
Spor; Jesse Stombaugh; James M. Tiedje; Doyle V. Ward; George M. Weinstock; Doug Wendel; Owen White; Andreas Wilke;
Jennifer Wortmann; Frank Oliver Glöckner.
We present the Genomic Standards Consortium’s (GSC) “Minimum Information about an
ENvironmental Sequence” (MIENS) standard for describing marker genes. Adoption of MIENS will enhance our
ability to analyze natural genetic diversity across the Tree of Life as it is currently being documented by massive DNA sequencing
efforts from myriad ecosystems in our ever-changing biosphere.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics; Earth & Environment;
Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5252/version/2
NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil,
Proteins and Water Contents
Provedor de dados: 17
Autores: I. C. Baianu; Jun Guo.
Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared
(NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed
composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique
development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert
and Beer’s law, and quantitative measurements can be successfully made with high speed and ease of operation.
NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the
food industry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6611/version/1
Complex Systems Analysis of Arrested Neural Cell Differentiation during Development and
Analogous Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: V. I. Prisecaru; I C. Baianu.
A new approach to the modular, complex systems analysis of nonlinear dynamics of arrested neural cell Differentiation--induced
cell proliferation during organismic development and the analogous cell cycling network transformations involved in
carcinogenesis is proposed. Neural tissue arrested differentiation that induces cell proliferation during perturbed development and
Carcinogenesis are complex processes that involve dynamically inter-connected biomolecules in the intercellular, membrane,
cytosolic, nuclear and nucleolar compartments. Such 'dynamically inter-connected' biomolecules form
numerous inter-related pathways referred to as 'molecular networks'. One such family of signaling
pathways contains the cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7101/version/2
Soybean Composition Database from NIR, NMR and GC-MS Analyses- (v.3)
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Jun Guo; Doina M. Costescu; V. I. Prisecaru.
This novel Soybean Composition Database from the AFC-NMR & NIR Spectroscopy Facility of the College of ACES
at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean
Germplasm Collection, such as those received from Peking at the National Soybean Collection.
Excel files
(.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made
available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based
software. 

A detailed account is also presented of our high-resolution nuclear
magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/3
In silico Genetic Network Models for Pre-clinical Drug Prioritization
Provedor de dados: 17
Autores: Jianghui Xiong; Juan Liu; Simon Rayner; Ze Tian; Yinghui Li; Shanguang Chen.
The high rates of failure in oncology drug clinical trials highlight the problems of using pre-clinical data to predict the clinical
effects of drugs. Patient population heterogeneity and unpredictable physiology complicate pre-clinical cancer modeling efforts.
We hypothesize that gene networks associated with cancer outcome in heterogeneous patient populations could serve as a
reference for identifying drug effects. Here we propose a novel in vivo genetic interaction which we call
‘synergistic outcome determination’ (SOD), a concept similar to ‘Synthetic
Lethality’. SOD is defined as the synergy of a gene pair with respect to cancer patients' outcome, whose
correlation with outcome is due to...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5343/version/1
USP4 is regulated by Akt phosphorylation and deubiquitylates TGF-beta type I receptor
Provedor de dados: 17
Autores: Long Zhang; Fangfang Zhou; Yvette Drabsch; Ewa Snaar-Jagalska; Craig Mickanin; Huizhe Huang; Kelly-Ann
Sheppard; Chris Lu; Peter ten Dijke.
Stability and membrane localization of Transforming growth factor-β (TGF-β) type I receptor
(TβRI)
is
essential
for
controlling
TGF-β
signaling.
TβRI
is
targeted
for
ubiquitination-mediated degradation by Smad7/Smurf2 complex. However, it is unclear whether polyubiquitin modified
TβRI can be reversed. Here we performed a genome-wide gain of function screen and identified ubiquitin-specific
protease (USP) 4 as a strong inducer of TGF-β signaling. Putative oncogenic USP4 was found to interact with
TβRI as deubiquitinating enzyme thus maintains TβR1 levels at the plasma membrane. Depletion of
USP4 mitigates TGF-β-induced breast cancer cell migration, epithelial...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Molecular Cell Biology;
Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6804/version/1
The “Minimum Information about an ENvironmental Sequence”
(MIENS) specification
Provedor de dados: 17
Autores: Pelin Yilmaz; Renzo Kottmann; Dawn Field; Rob Knight; James R. Cole; Linda Amaral-Zettler; Jack A. Gilbert; Ilene
Karsch-Mizrachi; Anjanette Johnston; Guy Cochrane; Robert Vaughan; Christopher Hunter; Joonhong Park; Norman
Morrison; Phillipe Rocca-Serra; Peter Sterk; Mani Arumugam; Laura Baumgartner; Bruce W. Birren; Martin J. Blaser; Vivien
Bonazzi; Peer Bork; Pier Luigi Buttigieg; Patrick Chain; Elizabeth K. Costello; Heather Huot-Creasy; Peter Dawyndt; Todd
DeSantis; Noah Fierer; Jed Fuhrman; Rachel E. Gallery; Richard A. Gibbs; Michelle Gwinn Giglio; Inigo San Gil; Elizabeth M.
Glass; Antonio Gonzalez; Jeffrey I. Gordon; Robert Guralnick; Wolfgang Hankeln; Sarah Highlander; Philip Hugenholtz; Janet
Jansson; Jerry Kennedy; Dan Knights; Omry Koren; Justin Kuczynski; Nikos Kyrpides; Robert Larsen; Christian L. Lauber;
Teresa Legg; Ruth E. Ley; Catherine A. Lozupone; Wolfgang Ludwig; Donna Lyons; Eamonn Maguire; Barbara A.
Methé; Folker Meyer; Sara Nakielny; Karen E. Nelson; Diana Nemergut; Josh D. Neufeld; Norman R. Pace;
Giriprakash Palanisamy; Jörg Peplies; Jane Peterson; Joseph Petrosino; Lita Proctor; Jeroen Raes; Sujeevan
Ratnasingham; Jacques Ravel; David A. Relman; Susanna Assunta-Sansone; Lynn Schriml; Erica Sodergren; Aymé
Spor; Jesse Stombaugh; James M. Tiedje; Doyle V. Ward; George M. Weinstock; Doug Wendel; Owen White; Andreas Wilke;
Jennifer Wortmann; Frank Oliver Glöckner.
We present the Genomic Standards Consortium’s (GSC) “Minimum Information about an
ENvironmental Sequence” (MIENS) standard for describing marker genes. Adoption of MIENS will enhance our
ability to analyze natural genetic diversity across the Tree of Life as it is currently being documented by massive DNA sequencing
efforts from myriad ecosystems in our ever-changing biosphere.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics & Genomics; Microbiology; Bioinformatics; Earth & Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/5252/version/1
The Neonatal Microbiome and Necrotizing Enterocolitis.
Provedor de dados: 17
Autores: Phillip I. Tarr; Barbara Warner; Erica Sodergren; William Shannon; Aaron Hamvas; Vincent Magrini; George
Weinstock.
Necrotizing enterocolitis (NEC) is a devastating disorder that affects approximately 10% of premature infants. Its mortality
remains high (15-30%), and its cause remains unknown. About 80% of cases occur within 35 days of birth among hospitalized
newborns of low birth weight. Probiotics diminish the incidence and severity of NEC, and NEC does not occur antepartum. NEC
affects a readily identifiable at-risk group, has a tightly defined interval before its onset, occurs in an organ system that is
intimately associated with a microbial population in flux, has a plausible association with the intestinal microbiota, and cohorts at
risk have rarely been studied in large numbers, or prospectively. This disorder, therefore, provides a unique opportunity to
explore...
Tipo: Marker Paper / Data Plan
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Microbiology; Bioinformatics; Data
Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5285/version/1
Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation
Provedor de dados: 17
Autores: I C. Baianu.
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this
article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes. Related
developments of these technique involve applications of Fluorescence Cross-Correlation Spectroscopy (FCCS) detection to
monitoring: 
DNA- telomerase interactions, DNA hybridization kinetics, ligand-receptor interactions, and
HIV-HBV testing. 

Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology;
Bioinformatics; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7116/version/1
Diagnostic robustness of DNA microarrays in the classification of acute leukemia
Provedor de dados: 17
Autores: Marie Loh; Adrian Mondry.
Countless statistical methods have been described for the analysis of DNA microarrays, and each yields distinct results. This
raises the question whether DNA microarrays are robust diagnostic tools.

In order to
address this issue, we compared five formally similar statistical tests for gene selection on a single data set derived from acute
leukemia patients. Inter-test agreement of gene selection, of sample classification and with standard clinical diagnosis was
calculated using Cohen's κ-score.

The inter-test agreement
scores were 0.15 < κ < 0.68 for gene selection, and 0.60 < κ
< 0.89 for sample classification....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1056/version/1
Near Infrared Reflectance Spectroscopy (NIRS) Determination of Isoflavone Contents for Selected
Soybean Accessions
Provedor de dados: 17
Autores: I. C. Baianu; Jun Guo; Tiefeng You; Randall
L. Nelson.
Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets. The rapid and
economical determination of soybean isoflavone concentrations is essential for the investigation and development of soybean
health foods as well as the selection of soybean seeds with optimal isoflavone levels for such foods. Fourier transforms near
infrared reflectance spectroscopy (FT-NIRS) calibrations were developed for the rapid and cost-effective analysis of isoflavones
in soybean seeds. FT-NIRS measurements were carried out in quadruplicate for 50 soybean lines selected from the USDA
Soybean Germplasm Collection. The selected soybean seeds provided a wide range of isoflavone concentrations (from 0.3 to 6.0
mg/g) that is...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7048/version/1
Structural and Dynamic Studies of Amino Acids, Proteins and Nucleic Acids By
Chemical-Hyperspectral Imaging, FT-IR/NIR, Vibro-Rotational Spectroscopy, Plasma, Microwave
and Neutron Spectroscopy
Provedor de dados: 17
Autores: I. C. Baianu.
A detailed review of structural and dynamic studies of amino acids, proteins and nucleic acids by spectroscopic techniques:
microwaves, near infrared, UV, X-rays and neutrons, including also EXAFS, chemical-hyperspectral imaging and fluorescence
correlation spectroscopy and FLIM, FRAP imaging with references, instrumentation diagrams and application examples for
several related areas.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Molecular Cell Biology; Bioinformatics; Data
Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7079/version/1
NMR and Electron Microscopy Studies of Soybean Seeds, Soybean Proteins, and Oil for Improved
Utilization in Foods and Feeds
Provedor de dados: 17
Autores: Doina M. Costescu; I. C. Baianu.
NMR, NIR and EM techniques are suitable methods of measuring protein and oil content in soybeans. After completion of a
measurement with NMR or NIR, a soybean sample could be either replanted or consummated. NMR techniques involved in the
present work are based on the response of nuclei, as nuclear spin flips, when set in a 7.05T magnetic field and excited with rf
pulses of 300 MHz. NIR spectroscopy is based on outer electron molecular vibrations, when excited with radiation of frequency
1012 -4×1014Hz, and =2.5Om-750 nm. 
TEM and ESEM techniques are bulk and
surface analysis techniques, respectively, working with electron beams of 15KV, and image resolution in the range of
nm’s. ESEM is based on the...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Plant
Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6192/version/1
Dual Diode Array and Fourier Transform Near Infrared Reflectance Spectrometer Calibrations for
Composition Analysis of Single Soybean Seeds for Genetic Selection, Cross-Breeding Experiments
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Randall L. Nelson.
Experimental results of extensive evaluations of Diode-Array and FT-NIRS instruments are presented that aim at developing
reliable and robust NIR calibrations for single soybean seed composition analysis on both Dual Diode Array and Fourier
Transform NIR reflectance instruments. Single soybean seed, bulk, and powder calibrations were developed on four different
NIRS spectrometer models that are commercially available.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6195/version/1
Open flower segregants selected from cajanus platycarpus crosses
Provedor de dados: 30
Autores: Cherian, C.A.
Palavras-chave: Pigeonpeas; Self pollination; Cytoplasm; Cross pollination; Segregation; Crossing over; Biotechnology;
Hybridization; Hybrids; Grain.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3187
Research need assessment and agricultural research priorities for South and West Asia
Provedor de dados: 30
Autores: M.C.S.
Palavras-chave: Agroclimatic zones; Biotechnology; Policies; Agroecosystems; Rice; Agroecology; Economic indicators;
Postharvest technology; Grain legumes; Capacity building.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4237
Germinating the seeds of success in the semi-arid tropics
Provedor de dados: 30
Autores: William D.
Palavras-chave: Pigeonpeas; Sorghum; Millets; Chickpeas; Transgenics; Biotechnology; Electrophoresis; Groundnuts;
Agroecosystems; Genetic transformation.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4145
Transformation and Regeneration of Groundnut, and Utilization of Viral Genes to Induce Resistance
to Virus Diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Genes; RNA; Transgenics; Protoplasts; Groundnuts; Transgenic plants; Biotechnology; Regeneration; Genomes;
Nucleotides.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4105
Towards a Grey to Green Revolution
Provedor de dados: 30
Autores: W.D.
Palavras-chave: Green revolution; Chickpeas; Biotechnology; Pigeonpeas; Watershed management; Private sector; Groundnuts;
Watersheds; Tropical zones; Sorghum.
Ano: 2002
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3727
Genes are gems:reporting agri-biotechnology
Provedor de dados: 30
Autores: R.L.
Palavras-chave: Biotechnology; Transgenics; Genes; DNA; Mycotoxins; Chickpeas; Transgenic plants; Genetic markers;
Genetic engineering; Genomes.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4146
Sharing perspectives on public-private sector interaction
Provedor de dados: 30
Autores: A.J.
Palavras-chave: Private sector; Hybrids; Policies; Biotechnology; Genetic resources; Breeds (animals); Millets; Agreements;
Patents; Sugarcane.
Ano: 2001
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4226
Development of new simple sequence repeat markers for pearl millet
Provedor de dados: 30
Autores: Yadav, O.P.
Palavras-chave: Locus; DNA; Genetic structures; PCR; Millets; Polymorphism; Genomes; Biotechnology; Breeds (animals);
Melting.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3309
Impact of Allium sativum leaf lectin on the Helicoverpa armigera larval parasitoid Campoletis
chlorideae
Provedor de dados: 30
Autores: Arora, R.,( Richa Arora ).
Palavras-chave: Animal developmental stages; Parasites; Transgenics; Biological interaction; Chickpeas; Biotechnology; Genes;
Garlic; Proteins; Grain legumes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3288
Future of Agriculture in the Semi-Arid Tropics
Provedor de dados: 30
Autores: M.C.S.
Palavras-chave: Water harvesting; Land resources; Tropical zones; Policies; Products; Biotechnology; Soil degradation;
Sustainable agriculture; Electrophoresis; Agricultural trade.
Ano: 2001
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3696
ICRISAT in the 21st century: towards sustainable food security
Provedor de dados: 30
Palavras-chave: Pigeonpeas; Genetic resources; Postharvest technology; Millets; Biotechnology; Green revolution; Tropical
zones; Hybrids; Policies; Integrated pest management.
Ano: 1998
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4210
Asian soybean rust in Brazil: past, present, and future
Provedor de dados: 86
Autores: Godoy,Cláudia Vieira; Seixas,Claudine Dinali Santos; Soares,Rafael Moreira; Marcelino-Guimarães,Franscismar
Correa; Meyer,Maurício Conrado; Costamilan,Leila Maria.
Abstract: Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is the most severe disease of the crop and can cause
yield losses of up to 90%. The disease was first reported in Brazil in 2001. Epidemics of the disease are common in the country,
where the fungus can survive year-round. Regulatory measures to reduce the inoculum between seasons and avoid late-season
soybean have been adopted to manage the disease. Disease control has relied mainly on chemical control, but a lower sensibility
of the fungus to fungicides has been reported in Brazil. Major-resistance genes have been mapped and incorporated into the
cultivars. With the reduced efficacy of the fungicides, the adoption of integrated measures to control the disease will be important
for...
Tipo: Journal article
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Biotechnology; Chemical control; Genetic resistance; Losses;
Management.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500407
Genetically modified trees: techniques, applications, risk and potential impacts associated with their
use
Provedor de dados: 190
Autores: Valdetaro, Erlon Barbosa; Carvalho, Ana Paula Vilela; Gamba, Otávio Surian; Demolinari, Michelle de Sales Moreira;
Silva, Elias.
Forest species have great economic and social importance, offering various products as wood and non-timber fundamental to
society. In this context, the search for new technologies that promote increase in productivity of commercial forests has received
special attention and biotechnology has contributed substantially to this. This branch of science can promote direct modification of
the genome of a target organism, through the analysis and manipulation of DNA genome or by insertion of fragments of the same
with known function and thus to achieve desired characteristics, such as resistance to diseases and pests, lignin reduction in wood
and generation of plants tolerant to different kinds of stresses. This review aims at reporting techniques for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic biotechnology forestry environmental impact. Transgênico; Biotecnologia; Setor florestal;
Impacto ambiental.
Ano: 2011
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/168
Comparing Consumer Attitudes towards Genetically Modified Food in Europe
Provedor de dados: 31
Autores: Springer,
A.; Mattas,
Konstadinos; Papastefanou,
G.; Tsioumanis,
Asterios.
As biotechnology evolves new methods of genetic engineering are now being applied to the production and processing of foods.
This paper is trying to explore the attitudes of the European consumers towards genetic modification of food. Using survey data of
the EU member countries the proposed research paper is planned to have a threefold output: 1) providing a comparative ranking of
the EU member countries in relation to the prevalence of rejection of genetically modified food, 2) uncovering intra-european
differences in genetic food engineering rejection as being based on socio-demographic and informational resp. knowledge based
differentials between EU countries and 3) specifying the importance of socio-economic and informational determinants of a...
Tipo: Conference Paper or Presentation
Palavras-chave: Genetic modification; Attitudes towards genetic modification; Biotechnology; Food; Consumer/Household
Economics.
Ano: 2002
URL: http://purl.umn.edu/24858
COMPETITIVE STRATEGIES OF BIOTECHNOLOGY FIRMS: IMPLICATIONS FOR U.S.
AGRICULTURE
Provedor de dados: 31
Autores: Begemann,
Brett D.
The agricultural biotechnology industry has evolved from a focus on outstanding science to a more mature phase where firms
focus on near-term products and building businesses. Understanding complex relationships and distribution channels and a
global perspective are crucial to commercialization. Yet, leading-edge technology and early identification of key traits will be
critical to developing superior products that ensure competitiveness in the marketplace.
Monsanto is organizing around a life
sciences model where seed, crop chemicals, pharmaceuticals, and food ingredient businesses will exploit mutual synergies driven
by basic science and discovery.
Tipo: Journal Article
Palavras-chave: Biotechnology; Monsanto; Strategies; Research and Development/Tech Change/Emerging Technologies.
Ano: 1997
URL: http://purl.umn.edu/15540
Chapter 07: USING CONJOINT ANALYSIS TO ASSESS CONSUMERS' ACCEPTANCE OF
PST-SUPPLEMENTED PORK
Provedor de dados: 31
Autores: Halbrendt,
Catherine K.; Pesek,
John D., Jr.; Parsons,
April; Lindner,
Robert K.
This book was originally published by Westview Press, Boulder CO, 1995.
Tipo: Book Chapter
Palavras-chave: Biotechnology; Pork products; Conjoint analysis; Willingness to buy; Food Consumption/Nutrition/Food Safety.
Ano: 1995
URL: http://purl.umn.edu/25973
A gap analysis of confined field trial application forms for genetically modified crops in East Africa:
evaluating the potential for harmonization
Provedor de dados: 31
Autores: Linacre,
Nicholas A.; Cohen,
Joel I.
The regulatory approval of genetically modified crops in the field initially requires small, restricted experimental trials known as
confined field trials. These small scale experiments provide researchers with important information on environmental interactions
and agronomic performance of the crop in a safe and contained manner. To authorize confined field trials regulatory review is
required, with formats for obtaining relevant information differing from country to country. In this paper, a Gap Analysis is used
to identify informational gaps and potential for harmonization of confined field trial application processes in three East African
countries - Tanzania, Kenya, and Uganda. The basic principle behind gap analysis is a comparison of the status quo...
Tipo: Report
Palavras-chave: Genetically modified crops; Gap analysis; Confined field trials; Biotechnology; Biosafety; Crop
Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55421
The Economic Value of Patents, Licenses, and Plant Variety Protection
Provedor de dados: 31
Autores: Rausser,
Gordon C.; Small,
Arthur A.
While biotechnology creates new opportunities for agriculture, developments are impeded by confusion in the system for
awarding intellectual property rights (IPRs) over agro-biotechnological innovations. An intelligent redesign IPR system requires
attention to how the definition of rights interacts with the market environment, generating incentives to create value.
A
distinction is drawn between cost-reducing innovations that increase the efficiency of producing homogeneous outputs and
value-adding innovations that create entirely new types of differentiated outputs. A reform is proposed that would require the
mandatory sublicensing of genes and certain core enabling technologies for creating genetically altered organisms.
Tipo: Working or Discussion Paper
Palavras-chave: Agriculture; Biotechnology; Intellectual Property; Market structure; Research and Development/Tech
Change/Emerging Technologies.
Ano: 1996
URL: http://purl.umn.edu/25023
ECONOMIC EFFECTS OF PURITY STANDARDS IN BIOTECH LABELING LAWS
Provedor de dados: 31
Autores: Giannakas,
Konstantinos; Kalaitzandonakes,
Nicholas G.
This paper develops a model of heterogeneous consumer preferences to analyze the market and welfare effects of reduced purity
standards for non-GM labeled food. Analytical results show that purity standards affect the equilibrium prices and quantities of
both the GM and non-GM products as well as the welfare of the groups involved. A change in purity standards is shown to create
winners and losers among the consumers as well as among the suppliers of the GM and conventional products.
Tipo: Conference Paper or Presentation
Palavras-chave: Agricultural biotechnology; Genetically modified products; Labeling; Purity standards; Consumer/Household
Economics.
Ano: 2005
URL: http://purl.umn.edu/19393
AN ERA OF CONFUSION: THE LAND GRANT RESEARCH AGENDA AND BIOTECHNOLOGY
Provedor de dados: 31
Autores: Weatherspoon,
Dave D.; Oehmke,
James F.; Raper,
Kellie Curry.
The Land Grant College and University (LGCU) research system is in a state of confusion. Public research privatization, industry
concentration, and balancing money and the Land Grant mission are issues LGCUs currently face. The ag-biotechnology
revolution complicates these issues. This paper provides four mission related policy recommendations for the LGCU system.
Keywords: Biotechnology, Land Grant Mission, Research
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Land Grant Mission; Research; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2000
URL: http://purl.umn.edu/11559
Genetically Modified Crops and Labor Savings in US Crop Production
Provedor de dados: 31
Autores: Gardner,
Justin G.; Nelson,
Carl H.
In spite of widespread adoption there is mixed evidence as to whether or not adopting Genetically Modified (GM) crops increase
farm welfare. One possible reason for widespread adoption is labor savings. Using a treatment effect model we estimate the labor
savings associated with adopting a GM crop.
Tipo: Conference Paper or Presentation
Palavras-chave: Genetically modified crops; Agricultural biotechnology; Endogeneity; Treatment effects; Survey weights; Crop
Production/Industries.
Ano: 2007
URL: http://purl.umn.edu/34919
SLAUGHTERHOUSE RULES: HUMAN ERROR, FOOD SAFETY, AND UNIFORMITY IN MEAT
PACKING
Provedor de dados: 31
Autores: Hennessy,
David A.
Meat retailers and processors express demand for a more uniform product, and technical innovations are allowing an increasingly
uniform supply. Meat packers can promote uniformity through pre-slaughter sorting, or earlier through contractual procurement.
Emphasizing human error and the efficacy of effort on the packing line, we develop a model whereby packers gain from
expanding revenue and reducing processing costs when exogenously determined carcass uniformity increases. Line speed and
occupational risk increase with uniformity. Whether optimally regulated or not, equilibrium food safety can decline with increased
uniformity. Effort-saving automation also will have an adverse effect on occupational safety, and may have this effect on
equilibrium food...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Contract provisions; Overload; Safety regulation; Value of information; Agricultural and Food
Policy; Livestock Production/Industries.
Ano: 2003
URL: http://purl.umn.edu/18625
GENETICALLY ENGINEERED CROPS FOR PEST MANAGEMENT IN U.S. AGRICULTURE
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge; McBride,
William D.
Adoption of genetically engineered crops with traits for pest management has risen dramatically since their commercial
introduction in the mid-1990's. The farm-level impacts of such crops on pesticide use, yields, and net returns vary with the crop
and technology examined. Adoption of herbicide-tolerant cotton led to significant increase in yields and net returns, but was not
associated with significant changes in herbicide use. On the other hand, increase in adoption of herbicide-tolerant soybeans led to
small but significant increases in yields, no changes in net returns, and significant decreases in herbicide use. Adoption of Bt
cotton in the Southeast significantly increased yields and net returns and significantly reduced insecticide use.
Tipo: Report
Palavras-chave: Biotechnology; Genetic engineering; Pest management; Field crops; Input traits; Crop Production/Industries.
Ano: 2000
URL: http://purl.umn.edu/33931
The Effects of Vertical Organization on the Pricing of Differentiated Products
Provedor de dados: 31
Autores: Shi,
Guanming; Chavas,
Jean-Paul.
We investigate differentiated product pricing and the effects of vertical organization under imperfect competition. We rely on
vertical measures of concentration (termed VHHI) to study how the exercise of market power varies with
substitution/complementarity relationships among products and vertical structures. This approach is applied to U.S. soybean seed
pricing under vertical integration versus licensing. We find evidence that vertical organization affects seed prices, with an impact
ranging from 1.87% to 13.6% of the mean price. These effects vary by institutional setup.We also find that complementarity can
mitigate price enhancements associated with market concentration.
Tipo: Article
Palavras-chave: Biotechnology; Imperfect competition; Pricing; Seed; Soybean; Vertical structures; Demand and Price Analysis;
Industrial Organization.
Ano: 2011
URL: http://purl.umn.edu/119157
Differing U.S. and European Perspectives on GMOs: Political, Economic and Cultural Issues
Provedor de dados: 31
Autores: Runge,
C. Ford; Bagnara,
Gian Luca; Jackson,
Lee Ann.
This paper provides an overview of the historical and cultural factors that have contributed to divergent U.S. and European views
on GMOs, and to resulting different national regulatory approaches for these products, specifically labelling policy. Within the
context of the international trading system, these national policy choices will have impacts that will spill over national borders.
Dialogue may be difficult to achieve, given widely divergent views concerning GMOs; however, without dialogue potential global
social benefits of policy harmonization will be forfeited.
Tipo: Journal Article
Palavras-chave: Biotechnology; Culture; Harmonization; Labelling; Trade; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2001
URL: http://purl.umn.edu/23874
A Meta-Analysis of Genetically Modified Food Valuation Studies
Provedor de dados: 31
Autores: Lusk,
Jayson L.; Jamal,
Mustafa; Kurlander,
Lauren; Roucan,
Maud; Taulman,
Lesley.
A plethora of research in recent years has been devoted to estimating consumer demand for genetically modified food, an
important piece of information needed to create appropriate public policy. To examine this body of work, a meta-analysis was
conducted of 25 studies that, in aggregate, report 57 valuations for GM food. Findings indicate as much as 89% of the variation in
existing value estimates for genetically modified food can be explained by an econometric model that controls for (a) the
characteristics of the sample of consumers studied, (b) the method for eliciting consumers' valuation, and (c) characteristics of the
food being valued. Each of these factors has a statistically significant effect on estimated premiums for non-GM food. Results
of...
Tipo: Journal Article
Palavras-chave: Biotechnology; Consumer acceptance; Genetically modified food; Willingness to pay; Consumer/Household
Economics.
Ano: 2005
URL: http://purl.umn.edu/30782
COSTS AND RISKS OF TESTING AND SEGREGATING GM WHEAT - SUMMARY
Provedor de dados: 31
Autores: Wilson,
William W.; Dahl,
Bruce L.
Development of genetically modified crops is challenging the functions of the grain marketing system with many participants
arguing for Identity Preservation (IP) systems prior to release of GM varieties. In this study, a stochastic optimization model was
developed to determine optimal testing strategies.
The model chooses the optimal testing strategy that maximizes utility
(minimizes disutility) of additional system costs due to testing and rejection and allows estimation of the risk premium required
for sellers to undertake a dual marketing system with GM/Non-GM segregations over the current Non-GM system. Elements of
costs (testing costs, rejection costs, and risk premium) were estimated for a base model representing a grain export chain. The
model...
Tipo: Working or Discussion Paper
Palavras-chave: Genetically modified organisms; Biotechnology; Wheat; Risk; Segregation; Identity preservation; Crop
Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/23502
CONSUMER RISK PERCEPTION PROFILES FOR THE FOOD-RELATED BIOTECHNOLOGY,
RECOMBINANT BOVINE GROWTH HORMONE (rbGH)
Provedor de dados: 31
Autores: Grobe,
Deana; Douthitt,
Robin; Zepeda,
Lydia.
Tipo: Conference Paper or Presentation
Palavras-chave: Consumer risk perception; Biotechnology; Bovine growth hormone; Food Consumption/Nutrition/Food Safety.
Ano: 1997
URL: http://purl.umn.edu/25953
The timing of evaluation of genebank accessions and the effects of biotechnology
Provedor de dados: 31
Autores: Koo,
Bonwoo; Wright,
Brian D.
The lack of ex-ante evaluation of germplasm in genebanks has been the single most prevalent and long-standing complaint of
plant breeders about the management of genebanks. Advances in biotechnology offer the possibility of faster, cheaper, and more
efficient evaluation methodologies. Will these new technologies favor ex-post evaluation, as some expect, or will it lead to more
ex-ante evaluation? Will it also lead to earlier development of varieties with disease resistance traits in anticipation of actual
infestations? Will the prospect of further advances in biotechnology favor delay of evaluation and development? This paper
addresses these questions in the case of evaluation of germplasm for resistance to a disease.
Tipo: Working or Discussion Paper
Palavras-chave: Gene banks; Plant; Biotechnology; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/97514
The Economics of Testing for Biotech Grain: Application to StarLink Corn
Provedor de dados: 31
Autores: Johnson,
D. Demcey; Lin,
William W.
StarLink corn, a variety not approved for human use, disrupted the marketing system in 2000 because of inadvertent
commingling. This paper provides an overview of the economics of testing grain for biotech content. What are the risks facing
buyers and sellers, and how are these influenced by testing protocols? How do market premiums and discounts, testing costs, and
prior beliefs affect the incentives to test? A conceptual model is developed in which sellers choose whether to pre-test grain prior
to shipment. Through simulation analysis, we illustrate the impact of market premiums and other variables on testing incentives
and buyer risk.
Tipo: Journal Article
Palavras-chave: Biotechnology; Grain marketing; Quality risk; StarLink; Testing; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/31220
COSTS AND RISKS OF TESTING AND SEGREGATING GM WHEAT
Provedor de dados: 31
Autores: Wilson,
William W.; Dahl,
Bruce L.
Development of genetically modified crops is challenging the functions of the grain marketing system with many participants
arguing for Identity Preservation systems prior to release of GM varieties. In this study, a stochastic optimization model was
developed to determine optimal testing strategies.
The model chooses the optimal testing strategy that maximizes utility
(minimizes disutility) of additional system costs due to testing and rejection and allows estimation of the risk premium required
for sellers to undertake a dual marketing system with GM/Non-GM segregations over the current Non-GM system. Elements of
costs (testing costs, rejection costs, and risk premium) were estimated for a base model representing a grain export chain. The
model...
Tipo: Working or Discussion Paper
Palavras-chave: Genetically modified organisms; Biotechnology; Wheat; Risk; Segregation; Identity preservation; Crop
Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/23480
On the Segregation of Genetically Modified, Conventional, and Organic Products in European
Agriculture: A Multi-market Equilibrium Analysis
Provedor de dados: 31
Autores: Moschini,
GianCarlo; Bulut,
Harun; Cembalo,
Luigi.
Evaluating the possible benefits of the introduction of genetically modified (GM) crops must address the issue of consumer
resistance as well as the complex regulation that has ensued. In the European Union (EU) this regulation envisions the
"co-existence" of GM food with conventional and quality-enhanced products, mandates the labelling and traceability of GM
products, and allows only a stringent adventitious presence of GM content in other products. All these elements are brought
together within a partial equilibrium model of the EU agricultural food sector. The model comprises conventional, GM and
organic food. Demand is modelled in a novel fashion, whereby organic and conventional products are treated as horizontally
differentiated but GM products are...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Differentiated demand; Genetically modified crops; Identity preservation; Innovation; Welfare;
Production Economics.
Ano: 2005
URL: http://purl.umn.edu/18531
MARKET STRUCTURE IN BIOTECHNOLOGY: IMPLICATIONS FOR LONG-RUN
COMPARATIVE ADVANTAGE
Provedor de dados: 31
Autores: Lavoie,
Brian F.; Sheldon,
Ian M.
A country specializing in a high technology industry may find that excess returns stemming from innovation are reallocated
overseas as foreign-based multinationals access ongoing domestic R&D through alliances with or acquisition of established
domestic start-ups. Computer simulation illustrates this process in the context of the current US specialization in biotechnology.
Keywords: Biotechnology, market structure, comparative advantage
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Market structure; Comparative advantage; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2000
URL: http://purl.umn.edu/21835
Analysis of Consumer Preferences for Biotech Labeling Formats
Provedor de dados: 31
Autores: Harrison,
R. Wes; Mclennon,
Everald.
Conjoint analysis is used to measure the preferences of United States consumers for labeling of biotech foods. The study found
that consumers in the sample support mandatory labeling of biotech foods. This suggests that U.S. consumers would support
revisions to the present voluntary labeling policy of the U.S. Food and Drug Administration and the U.S. Department of
Agriculture. Results also showed that the preferred labeling formal is a text disclosure that describes the benefits of biotechnology
in combination with a biotech logo.
Tipo: Journal Article
Palavras-chave: Agricultural Biotechnology; Labeling; Conjoint Analysis; Q18; Q13.
Ano: 2004
URL: http://purl.umn.edu/42937
An Empirical Analysis of the Effects of Plant Variety Protection Legislation on Innovation and
Transferability
Provedor de dados: 31
Autores: Srinivasan,
Chittur S.; Shankar,
Bhavani; Holloway,
Garth J.
Under the TRIPs Agreement, all member-countries of the World Trade Organization are required to provide an "effective" system
of plant variety protection within a specific time frame. In many developing countries this has led to a divisive debate about the
fundamental desirability of extending intellectual property rights to agriculture. But empirical studies on the economic impacts of
PVP, especially its ability to generate large private sector investments in plant breeding and facilitate the transfer of technology,
have been very limited. This paper examines two aspects of the international experience of PVP legislation thus far (i) The
relationship between legislation, R&D expenditures and PVP grants, i.e., the innovation effect, and (ii) The role...
Tipo: Conference Paper or Presentation
Palavras-chave: Plant variety protection; Biotechnology; Technology transfer; Crop Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/24788
Regulatory Approval Decisions in the Presence of Market Externalities: The Case of Genetically
Modified Wheat
Provedor de dados: 31
Autores: Furtan,
William Hartley; Gray,
Richard S.; Holzman,
J.J.
This study examines the optimal approval strategy for genetically modified (GM) wheat varieties in Canada and the United States.
Without an affordable segregation system, the introduction of GM wheat will create a market for "lemons" that will result in the
loss of important export markets. Using a differentiated product trade model for spring wheat, with endogenous technology
pricing, a payoff matrix is generated for the possible approval outcomes. Results show that the existence of the market externality
removes the first-mover advantage for wheat producers from the approval of the new GM wheat variety. There are large
distributional effects; wheat producers lose economic surplus, while consumers and the biotech company gain economic surplus.
With a...
Tipo: Journal Article
Palavras-chave: Biotechnology; Market externalities; Non cooperative games; Strategic approval decisions; Trade; Agricultural
and Food Policy.
Ano: 2005
URL: http://purl.umn.edu/30778
The Case For and Against Import Embargoes on Products of Biotechnology
Provedor de dados: 31
Autores: Gaisford,
James D.; Chui-Ha,
Carol Lau.
This article examines alternative trade policy responses available to an importing country with concerns over innovations in
biotechnology. Regardless of the policy response, the importing country may be worse off after a new genetically modified food
(GMF) is introduced. While an import embargo may be preferable to allowing free access to unlabelled GMF imports, permitting
labelled imports is typically superior to an embargo. Thus, import embargoes on products of biotechnology should not be
generally allowed. The paper provides surprising support for the existing WTO provisions on Technical Barriers to Trade, but
suggests significant potential problems with the recently negotiated Biosafety Protocol.
Tipo: Journal Article
Palavras-chave: Asymmetric information; Biotechnology; Genetically modified foods; Import embargo; Labelling; International
Relations/Trade.
Ano: 2000
URL: http://purl.umn.edu/23832
BIOREFINERIES AND BIOBASED PRODUCTS FROM THE CONSUMER'S POINT OF VIEW
Provedor de dados: 31
Autores: Kurka,
Stefan; Menrad,
Klaus.
Paper prepared for presentation at the 13th ICABR International Conference on Agricultural Biotechnology: “The emerging
bio-economy” Ravello (Italy), 18th to 20th June 2009
Tipo: Conference Paper or Presentation
Palavras-chave: Industrial biotechnology; Biorefinery; Consumer behaviour; Demand and Price Analysis; M39; R20.
Ano: 2009
URL: http://purl.umn.edu/91330
A Model of Diffusion of Genetically Modified Crop Technology in Concentrated Agricultural
Processing Markets - The Case of Soybeans
Provedor de dados: 31
Autores: Nadolnyak,
Denis A.; Sheldon,
Ian M.
In the paper, a dynamic model of diffusion of genetically modified crop technology is developed and simulated using the U.S.
soybean market data. The model accounts for factors specific to agricultural markets, such as oligopsony power and strategic
interaction among crop processors, growers' characteristics such as adoption behavior, and identity preservation requirements.
Simulation results show how these factors affect the magnitude and distribution of the potential gains from genetically modified
crops.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology in agriculture; Soybeans; Innovation diffusion; Oligopsony; Genetically modified crop;
Consumer/Household Economics.
Ano: 2002
URL: http://purl.umn.edu/24872
Producers and Consumer attitudes toward Biotechnology in Ghana
Provedor de dados: 31
Autores: Nsafoah,
Annie; Dicks,
Michael R.; Osei,
Collins.
Over 265 million people in Sub-Saharan Africa face malnutrition, chronic hunger, and poverty. One of the technologies that could
help alleviate the perpetuating cycle of chronic hunger is biotechnology. Genetic modification (GM) has the potential to enhance
agricultural productivity and improve Africa’s food security, but little is known about the potential benefits and costs of using
genetically modified maize in Africa - Ghana.
African and Ghanaian policy makers, farmers, and consumers often have
difficulty accepting new technologies. Their reluctance is due to the investment required for new technology, - aversion to risk,
the changes required to traditional production practices, and incomplete- knowledge of new technologies. This study elicits the...
Tipo: Conference Paper or Presentation
Palavras-chave: Producers and Consumer attitudes toward Biotechnology in Ghana; Agricultural and Food Policy; International
Development; International Relations/Trade.
Ano: 2011
URL: http://purl.umn.edu/98642
Conservation Tillage, Pesticide Use, and Biotech Crops in the U.S.A.
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge; Hallahan,
Charles B.; Nehring,
Richard F.; Wechsler,
Seth James; Grube,
Arthur.
This paper presents the first part of an ongoing project whose objective is to present a long term relationship between conservation
tillage, adoption of GE crops and pesticide use for major crops in the United States. In addition, the project aims to provide some
innovative tests on causality using a panel data set. This paper presents preliminary results for soybeans.
Tipo: Conference Paper or Presentation
Palavras-chave: Conservation tillage; Biotechnology; Genetically engineered crops; Soybeans; Herbicides; Environmental
Economics and Policy; Farm Management; Production Economics.
Ano: 2010
URL: http://purl.umn.edu/60941
Consumer Acceptance of Genetically Modified Food Products in the Developing World
Provedor de dados: 31
Autores: Curtis,
Kynda R.; Wahl,
Thomas I.; McCluskey,
Jill J.
World-wide consumer response toward food products made from genetically modified ingredients has been largely negative.
However, the majority of the previous studies on consumer attitudes towards genetically modified food products were conducted
in developed countries in Europe as well as Japan. The small number of studies conducted in developing countries obtained
different results from the developed world. This paper considers the motivations for consumer attitudes towards genetically
modified foods in developing countries. We conclude that the generally positive perception towards genetically modified foods in
developing countries could be the result of a cost-benefit analysis consistent with expected utility theory. Developing countries
have more...
Tipo: Conference Paper or Presentation
Palavras-chave: Developing countries; Risk; Biotechnology; Genetically modified foods; Crop Production/Industries; Risk and
Uncertainty.
Ano: 2003
URL: http://purl.umn.edu/57858
Основные направления развития биохимических исследований в Одесском филиале ИнБЮМ
НАН Украины
Provedor de dados: 13
Autores: Кандюк, Р. П.
Приводятся данные о развитии биохимических исследований в Одесском филиале
ИнБЮМ НАН Украины за
период с середины прошлого века до начала нынешнего столетия. Показано влияние антропогенных факторов на
обменные процессы гидробионтов и функционирование биохимических систем. Отмечена возможность практического
использования черноморских гидробионтов и получения из них лечебно-профилактических препаратов, пищевых и
кормовых добавок.
Tipo: Journal Contribution
Palavras-chave: Биохимические исследования; Биотехнологии; Biochemistry researches; Biotechnologies Biotechnology
Biochemistry Biotechnology Biochemistry http://aims.fao.org/aos/agrovoc/c_16165 http://aims.fao.org/aos/agrovoc/c_910.
Ano: 2003
URL: http://repository.ibss.org.ua/dspace/handle/99011/482
Antártica: fuente de recursos biológicos para la biotecnología nacional
Provedor de dados: 20
Autores: Blamey, J.
En diciembre del 2007, Innova-Chile de CORFO aprobó el financiamiento del proyecto “Antártica: Fuente de recursos biológicos
para la biotecnología nacional”. Este proyecto tiene como fin crear una plataforma que facilite el acceso a los recursos antárticos
de microorganismos y plantas, de una forma que permitirá su valorización para la biotecnología chilena y su desarrollo científico
en el área. Por sus únicas condiciones de aislamiento y bajas temperaturas, la Antártica alberga un potencial científico y
biotecnológico enorme, pero también muy altas barreras de entrada de diferente índole.
Tipo: Journal Contribution
Palavras-chave: Biotechnology; Microorganisms; Flora; Biotechnology; Microorganisms; Flora;
Http://aims.fao.org/aos/agrovoc/c_16165; Http://aims.fao.org/aos/agrovoc/c_4807; Http://aims.fao.org/aos/agrovoc/c_2983.
Ano: 2008
URL: http://hdl.handle.net/1834/3237
A nova era biotecnológica na agricultura: situação dos novos eventos.
Provedor de dados: 119
Autores: KARAM, D.; GAZZIERO, D. L. P.; VARGAS, L.
2015
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Planta tiguera; Planta daninha; Biotecnologia; Planta transgênica; Milho; Zea mays; Herbicida; Soja; Glycine
max; Biossegurança; Biotechnology; Transgenic plants; Herbicides; Biosecurity.
Ano: 2015
The brazilian core collection of cassava.
Provedor de dados: 119
Autores: CORDEIRO, C.M.T.; ABADIE, T.; FUKUDA, W.M.G.; BARRETO, J.F.; BURLE, M.L.; CARDOSO, E.M.R.;
CAVALCANTI, J.; COSTA, I.R.S.; FIALHO, J.F.; MAGALHAES, J.R.; MARSCHALEK, R.; ROCHA, D.M.S.; VALE, T.L.
The size of Germplasm Collections has become an important limitation for theirr use in plant breeding programs. To overcome
this limitation the Core Collection concept has been proposed. A Core Collection consists of a set of accessions selected to
represent the genetic diversity of the base collection with minimum repetitiveness. This insures the conservation of maximum
genetic variation, allowing rapid evaluation of germplasm and better access to the base collection. The brazilian germplasm
Collection of Cassava is the largest national collection, and contains strategic genetic variation for the development of breeding
programs worldwide. It consists of approximately 3350 accessions conserved in 7 regional Active Germplasm Banks. To develop
the Core...
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Mandioca; Banco de germoplasma; Variabilidade genetica; Melhoramento de planta; Biotecnologia; Manihot
esculenta; Cassava; Gene banks; Genetic variation; Plant breending; Biotechnology; Mandioca; Cassava.
Ano: 2000
URL: http://www.alice.cnptia.embrapa.br/handle/doc/134075
Estudo sobre a viabilidade da produção de linhagens de milho por meio de cultura de anteras.
Provedor de dados: 119
Autores: CARVALHO, C.H.S.; ANGELO, P.C. da S.; MAGNAVACA, R.; GAMA, E.E.G.e.
Este trabalho foi conduzido com os objetivos de estabelecer protocolos para o cultivo de anteras de milho, in vitro, visando a
producao de linhagens; e avaliar a possibilidade da incorporacao dessa tecnica em um programa de melhoramento. Foram testados
37 genotipos de milho. A metodologia utilizada inclui a determinacao do estadio de desenvolvimento dos microsporos, mediante
coloracao com carmim propionico; um teste de viabilidade de microsporos, pela reacao da fluorescencia enzimaticamente
induzida; o pre-tratamento de pendoes a 8,0 C; o plaqueamento das anteras, em posicao lateral, sobre tres tipos de meio; e a
incubacao das placas no escuro ou sob fotoperiodo de 16 horas, a 26 +/- 1C. Para a regeneracao de plantas, os calos foram
transferidos para um...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Milho in vitro; Anteras; Cultivo; Producao; Linhagens; Biologia molecular; Biotecnologia; Zea mays; Maize;
Molecular biology; Biotechnology; Plant in vitro; Production; Anther.
Ano: 1995
Tecnologias da informação e comunicação e suas relações com a agricultura.
Provedor de dados: 119
Autores: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S.
Visando promover o intercâmbio de conhecimento e estabelecer a participação das novas tecnologias sobre a competitividade e a
sustentabilidade da agricultura regional, a Embrapa Informática Agropecuária tomou a iniciativa de organizar a elaboração deste
livro que trata do uso das tecnologias da informação e comunicação e suas relações com a agricultura. Este é o fruto de um
trabalho conjunto com o Instituto Nacional de Tecnología Agropecuaria (Inta Argentina), Instituto Nacional de Investigaciones
Agropecuaria (Inia Chile), o Instituto Nacional de Investigación Agropecuaria (Inia Uruguay) e o Instituto Interamericano de
Cooperação para Agricultura (IICA-Procisur).
Tipo: Livros científicos (ALICE)
Palavras-chave: Automação das cadeias produtivas; Tecnologia da informação; Biotecnologia; Recursos naturais; Mudanças
climáticas; Information technology; Biotechnology; Natural resources; Climate change.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1012319
Protocolos hormonais de indução de estro sincronizado em ovelhas leiteiras na contra estação
reprodutiva.
Provedor de dados: 119
Autores: BALARO, M. F. A.; FONSECA, J. F. da; MAIA, A. L. R. S.; DELGADO, K. F.; FERREIRA, M. I. C.; BRANDÃO, F.
Z.
[Protocols hormonal to estrus induction and synchronization and pregnancy rate in dairy ewes out-of season mating].
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: Raça Lacaune; Ovino; Ovelha; Biotecnologia; Reprodução animal; Prenhez; Sincronização do cio; Ciclo estral;
Sheep; Ewe; Animal reproduction; Biotechnology; Pregnancy; Synchronization.
Ano: 2013
Celulose bacteriana obtida por diferentes fontes agroindustriais.
Provedor de dados: 119
Autores: LIMA, H. L.; NASCIMENTO, E. S.; BARROS, M. de O.; SANTIAGO, M. I. A.; PEREIRA, A. L. S.; BRIGIDA, A. I.
S.; MORAIS, J. P. S.; BORGES, M. de F.; ROSA, M. de F.
No presente trabalho caracterizou-se películas de celulose bacteriana (CB) obtidas por cultivo de Gluconacetobacter hansenii
ATCC 23769 utilizando suco de caju, extrato de algaroba e líquido de sisal como fontes alternativas. As películas obtidas não
diferiram do meio padrão sintético, usualmente utilizado para produzir CB, quando ao perfil de degradação térmica, pureza e
cristalinidade. Tais celuloses apresentaram índices de cristalinidade acima de 91%, temperatura máxima de degradação acima de
315°C e umidade acima de 97%.
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Biotecnologia; Polímero natural; Extrato de algaroba; Líquido de sisal; Suco de caju; Biotechnology; Natural
Polymer; Mesquite extract; Sisal juice; Cashew juice.
Ano: 2015
Accommodating the difference in students' prior knowledge of cell growth kinetics
Provedor de dados: 69
Autores: Seters,Janneke van; Ossevoort,Miriam; Goedhart,Martin; Tramper,Johannes.
This paper describes the development and benefits of an adaptive digital module on cell growth to tackle the problem of educating
a heterogeneous group of students at the beginning of an undergraduate course on process engineering. Aim of the digital module
is to provide students with the minimal level of knowledge on cell growth kinetics they need to comprehend the content
knowledge of the subsequent lectures and pass the exam. The module was organised to offer the subject matter in a differentiated
manner, so that students could follow different learning paths. Two student groups were investigated, one consisting of students
who had received their prior education abroad and one of students that had not. Exam scores, questionnaires, and logged user
data...
Tipo: Journal article
Palavras-chave: Adaptive digital learning material; Bioreactor design; Biotechnology; Education; Intelligent tutoring system;
Intelligent Web-based educational system.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200001
Biossegurança ambiental de OGMs.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: In 2005, there were 90 million hectares cultivated with GMOs by 8.5 million producers in 21 countries. In 2006, we
commemorated 10 years of commercial production of GMOs. Currently there are 10 million farmers planting 100 million
hectares. However, several points were raised concerning the environmental impact of GMOs: (1) risk of cultivated or wild hybrid
become a harmful invasive species, (2) development of resistance by the massive use of technology, (3) possibility of vertical or
horizontal gene flow, (4) adverse effect on non-target and beneficial species, (5) impacts on crop production systems. This article
discusses the main results on those concerns. The data obtained so far show that the environmental impacts of GMOs ares similar
to a...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Melhoramento genético vegetal; Biotecnologia; Planta transgênica; Genética vegetal; Segurança alimentar; Plant
breeding; Biotechnology; Transgenics; Genetics; Transgenic foods; Food security.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570248
Variabilidade genetica de plantas de Stylosanthes guianensis var. vulgaris com base em marcadores
RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; BARCELLOS, A. de O.; ANDRADE, R. P.; KARIA, C. T.; RAMOS, A. K. B.; COELHO, M. H.;
PINTO, J. F. N.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; RAPD; Variacao genetica; Leguminosa forrageira; Melhoramento genetico vegetal;
Genetic markers; Genetic variation; Feed legumes; Plant breeding; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567821
Similaridade genetica de acessos de arachis pintoi com diferentes niveis de produtividade de materia
seca com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; ANDRADE, R. P.; KARIA, C. T. CORDEIRO, M. C. R.; SOUZA, T. L. P. O.; MERIDA, J. L.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; RAPD; Amendoim; Planta forrageira; Variacao genetica; Biotecnologia; Genetic markers;
Peanut; Feed crops; Genetic variation; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/559244
Uso de ferramentas moleculares em estudos da diversidade de microrganismos do solo.
Provedor de dados: 14
Autores: REIS JUNIOR, F. B. dos; MENDES, I. de C.; TEIXEIRA, K. R. dos S.; REIS, V. M.
2002
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Microbiologia do solo; Populacao microbiana; Manejo do solo; Biotecnologia; Biologia molecular;
Polimorfismo; Soil; Microbiology; Microbial flora; Soil management; Biotechnology; Molecular biology; Polimorphism.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/559150
Diversidade genética de variedades comerciais de maracujazeiro-azedo com base em marcadores
moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BRAGA, M. F.; BELLON, G.; PEIXOTO, J. R.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Biotecnologia; Marcador molecular; Marcador genético; Maracujá; Passiflora edulis; Genetic
variation; Biotechnology; Genetic markers; Genetic variation; Passion fruits.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/544592
Extração de DNA e obtenção de marcadores moleculares para diferentes espécies de interesse para o
cerrado.
Provedor de dados: 14
Autores: BELLON, G.; FALEIRO, F. G.; BARROS, A. M.; KARIA, C. T.; ANDRADE, R. P.; CORDEIRO, M. C. R.; PINTO,
A. C.; JUNQUEIRA, N. T. V.; PEREIRA, A. V.; PEREIRA, E. B. C.; FERNANDES, F. D.; FERREIRA, M. E.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; DNA; Marcador genético; Biologia molecular; Biotecnologia; Cerrado; Planta nativa;
Genetic markers; Molecular biology; Biotechnology; Indigenous organisms.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/553057
Anais...
Provedor de dados: 14
Autores: SEMANA DA CAPRINOCULTURA E DA OVINOCULTURA TROPICAL BRASILEIRA, 1., 1994, Sobral.
Aspectos economicos da caprino-ovinocultura tropical brasileira; Caprinocultura leiteira no Brasil - estadio da arte e perspectivas;
Estadio atual e perspectivas da ovinocultura tropical; Biotecnologia da reproducao caprina; Tecnologia e comercializacao de carne
ovina; Industrializacao e comercializacao de pele; A modernidade cientifica e sua interfaces.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Aspecto econômico; Congresso; Brasil; Ceara; Congress; Brazil; Caprinocultura; Ovinocultura;
Comercialização; Mercado; Biotecnologia; Goats; Sheep; Commercialization; Mercado; Biotechnology; Small ruminants.
Ano: 1994
Distribuição da variabilidade genética Stylosanthes macrocephala coletadas na região de cerrado e
semi-árido brasileiro.
Provedor de dados: 14
Autores: BARROS, A. M.; FALEIRO, F. G.; KARIA, C. T.; SHIRATSUCHI, L. S.; ANDRADE, R. P.; LOPES, G. K. B.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador genético; Biotecnologia; Marcador molecular; RAPD; Leguminosa forrageira;
Genetic variation; Genetic markers; Biotechnology; Feed legumes.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567165
Fingerprinting analysis of mango (Mangifera indica L.) cultivars introduced or developed in Brazil
using RAPD markers.
Provedor de dados: 14
Autores: FALEIRO, F. G.; CORDEIRO, M. C. R.; PINTO, A. C. Q.; ROSSETTO, C. J.; BELLON, G.; ANDRSDE, S. R. M.;
FRAGA, L. M. S.; SOUZA, T. L. P. O.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador genetico; Manga; Mangifera indica; Marcador molecular; RAPD; Melhoramento genetico;
Biotecnologia; Brasil; Genetic markers; Mango; Plant breeding; Biotechnology; Brazil.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/566520
Transformacao de Phaseolus vulgaris Via SAAT ("Sonication-assisted agrobacterium- mediated
transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Biotecnologia; Transgenico; Melhoramento genetico vegetal; Feijao; Phaseolus vulgaris; Transformacao
genetica; Gene; Biotechnology; Transgenics; Plant breeding; Beans; Genetic transformation; Genetics.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567648
Transformacao de feijoeiro com gene Lhcb1*2 por meio do metodo SAAT
("Sonication-assited-agrobacterium transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Melhoramento genetico vegetal; Feijao; Phaseolus vulgaris; Transformacao genetica; Biotecnologia;
Transgenico; Gene; Plant breeding; Beans; Genetic transformation; Biotechnology; Transgenics.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567647
Biotecnologias aplicadas à reprodução de ovinos e caprinos.
Provedor de dados: 14
Autores: FONSECA, J. F. da; CRUZ, R. do C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G. de; VIANA, J. H. M.
A criação de caprinos e ovinos é uma atividade agropecuária de elevada importância social, econômica e consolidada em
determinadas regiões do Brasil, enquanto em outras, mostra-se em fase emergente. Diversos aspectos apontam para o
desenvolvimento desta cadeia produtiva no país. Dentre eles, a produção nacional, especialmente de carne e leite, sequer abastece
o mercado interno, mesmo considerando que o consumo ainda é reduzido em relação a outros países. Em função da qualidade e
diferencial dos produtos de origem caprina e ovina, acredita-se que em pouco tempo a demanda aumentará expressivamente,
requerendo sistemas de criação focados na produtividade. Assim, torna-se fundamental desenvolver e adequar tecnologias
voltadas ao incremento produtivo. Para...
Tipo: Livros técnicos (INFOTECA-E)
Palavras-chave: Transferência nuclear; Biotechnology; Cloning; Goats; Reproduction; Sheep; Transgenic animals;
Biotecnologia; Caprino; Clonagem; Inseminação artificial; Ovino; Reprodução animal; Sêmen.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/1027956
Principios da cultura de tecidos vegetais.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
2002
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Cultura de tecido; Biotecnologia; Melhoramento vegetal; Explante; Cultura in vitro; Regeneracao;
Micropropagacao; Tissue culture; Biotechnology; Plant breeding; Explants; In vitro culture; Regeneration; Micropropagation.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/546466
Variabilidade genética de acessos de maracujá-suspiro (Passiflora nitida Kunth.) com base em
marcadores moleculares.
Provedor de dados: 14
Autores: JUNQUEIRA, K. P.; FALEIRO, F. G.; RAMOS, J. D.; BELLON, G.; PAULA, M. da S.; JUNQUEIRA, N. T. V.;
BRAGA, M. F.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador genético; Biotecnologia; Marcador molecular; Maracujá; Passiflora edulis; Genetic
variation; Genetic markers; Biotechnology; Passion fruits.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570171
Operacionalizacao da extracao de DNA de especies nativas do cerrado visando analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; DNA; Marcador genetico; Cerrado; Planta nativa; Biologia molecular; Biotecnologia;
Genetic markers; Indigenous organisms; Molecular biology; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567935
Caracterizacao molecular e estabelecimento de colecao de trabalho de Stylosanthes macrocephala
com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; BARROS, A. M.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.; GOMES, A. C.;
ANDRADE, R. P.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; RAPD; Leguminosa forrageira; Variacao genetica; Biotecnologia; Biologia molecular;
Genetic markers; Feed legumes; Genetic variation; Biotechnology; Molecular biology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567912
Variabilidade genética de Mahanarva spectabilis (Distant, 19090) coletadas em diferentes locais e
hospedeiros, com base em marcadores moleculares RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; PAULA-MORAES, S. V. de; JUNQUEIRA, K. P.; BELLON, G.; RAMOS, A. K. B.; VILELA, M. de
F.; CARVALHO, G. S.; AUAD, A. M.; TAKADA, S.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Marcador genético; Biotecnologia; Marcador molecular; Praga de planta; Cigarrinha; Genetic
variation; Genetic markers; Biotechnology; Pests of plants.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570175
Biossegurança de alimentos transgênicos.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: The use of transgenic plants on the human diet is a matter of great concern for the scientific community and the
public in general. However, it is important to consider that before the release for human consumption, transgenic foods are
submitted to an extensive series of rigorous tests. These tests includes the characterization of the expressed protein, in vitro
digestibility and oral evaluation of acute toxicity in mice, studies of the structural homology of the protein with other known toxic
proteins, allergenic potential and nutritional equivalence. Questions about the true risks of transgenic food for human health
cannot be answered in general terms, but we can expect a lower potential risk when compared to another type of food that did
not...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Transgênico; Genética vegetal; Biotecnologia; Melhoramento genético vegetal; Segurança alimentar;
Transgenics; Genetics; Biotechnology; Plant breeding; Transgenic foods; Food security.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/559186
Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering
Provedor de dados: 5
Autores: Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline;
Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well
documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics.
Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have
shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of
structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been
discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical
modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf
Microalgues marines : les enjeux de la recherche
Provedor de dados: 5
Autores: Muller-feuga, Arnaud.
Industrial potentialities related to biotechnology of microalgae are examined, highlighting a wide spectrum of applications which
concern principally nutrition, health, environment and energy. The french situation of research on microalgae, especially at
Ifremer, is assessed, showing that the main axis are fine chemistry, aquaculture, environment, bio-process engineering. It covers
the majority of the applications, but leaves in shade certain important aspects such as human food and CO2 fixation. Concerning
the general approach, some significant improvements can be expected from a more accurate control of bacteriological and genetic
quality of cultures. About the industrial aspects, a national initiative seems in progress. The variety of potential...
Tipo: Text
Palavras-chave: Process engineering; Energy; Environment; Health; Food; Aquaculture; Biotechnology; Microalgae; Génie des
procédés; Energie; Environnement; Santé; Alimentation; Aquaculture; Biotechnologie; Microalgues.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1092.pdf
FOLy: An integrated database for the classification and functional annotation of fungal
oxidoreductases potentially involved in the degradation of lignin and related aromatic compounds
Provedor de dados: 189
Autores: Levasseur, A.; Piumi, F.; Coutinho, P.M.; Rancurel, C.; Asther, M.; Delattre, M.; Henrissat, B.; Pontarotti, P.; Asther,
M.; Record, E.
The breakdown of lignin by fungi is a key step during carbon recycling in terrestrial ecosystems. This process is of great interest
for green and white biotechnological applications. Given the importance of these enzymatic processes, we have classified the
enzymes potentially involved in lignin catabolism into sequence-based families and integrated them in a newly developed
database, designated Fungal Oxidative Lignin enzymes (FOLy). Families were defined after sequence similarity searches starting
from protein sequences and validated by the convergence of results with biochemical experiments reported in the literature. The
resulting database was applied as a tool for the functional annotation of genomes from different fungi, namely (i) the
Basidiomycota...
Tipo: Journal Article
Palavras-chave: COMPOSE AROMATIQUE; BASIDIOMYCETES; BASE DE DONNEES; ASCOMYCETES MELANIN
SYNTHESIS; FUNCTIONAL CLASSIFICATION; LIGNIN DEGRADATION; FUNGAL GENOME; FUNCTIONAL
ANNOTATION; DATABASE; GREEN BIOTECHNOLOGY.
Ano: 2008
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20087e29edda&uri=/notices/prodinra1/2008/06/
A Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for
Composition Improvements- (v.4)
Provedor de dados: 17
Autores: Valentin Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at
Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions. Conventional
“wet chemistry” analytical methods are time-consuming and costly. As a result, soybean breeders
and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive
method for composition analysis for academia and industry. Recent advancements in instrumentation design, such as the
application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly
improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell
Biology; Bioinformatics; Earth & Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/4
Determination of Soybean Oil, Protein and Amino Acid Residues in Soybean Seeds by High
Resolution Nuclear Magnetic Resonance (NMRS) and Near Infrared (NIRS)
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Doina Costescu; P R. Lozano; Valentin Prisecaru; Randall
L. Nelson.
A detailed account is presented of our high resolution nuclear magnetic resonance (HR-NMR) and near infrared (NIR) calibration
models, methodologies and validation procedures, together with a large number of composition analyses for soybean seeds. NIR
calibrations were developed based on both HR-NMR and analytical chemistry reference data for oil and twelve amino acid
residues in mature soybeans and soybean embryos. This is our first report of HR-NMR determinations of amino acid profiles of
proteins from whole soybean seeds, without protein extraction from the seed. It was found that the best results for both oil and
protein calibrations were obtained with a Partial Least Squares Regression (PLS-1) analysis of our extensive NIR spectral data,
acquired...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Ecology; Genetics & Genomics; Molecular Cell
Biology; Bioinformatics; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7053/version/1
Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for
Composition Improvements. (Version 2.0)
Provedor de dados: 17
Autores: Valentin Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at
Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions. Conventional
“wet chemistry” analytical methods are time-consuming and costly. As a result, soybean breeders
and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive
method for composition analysis for academia and industry. Recent advancements in instrumentation design, such as the
application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly
improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell
Biology; Bioinformatics; Earth & Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/2
Neural Networks, Cell Genome and Interactome Nonlinear Dynamic Models
Provedor de dados: 17
Autores: I. C. Baianu.
Operational logic and bioinformatics models of nonlinear dynamics in complex functional systems such as neural networks,
genomes and cell interactomes are proposed. Łukasiewicz Algebraic Logic models of genetic networks and signaling
pathways in cells are formulated in terms of nonlinear dynamic systems with n-state components that allow for the generalization
of previous logical models of both genetic activities and neural networks. An algebraic formulation of variable
'next-state
functions'
is
extended
to
a
Łukasiewicz
Topos
with
an
n-valued
Łukasiewicz Algebraic Logic subobject classifier description that represents non-random and nonlinear network
activities as well as their transformations in...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology;
Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6206/version/1
Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and
Embryos
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Doina M. Costescu.
Novel methodologies are currently being evaluated for the Chemical Analysis of soybean seeds as well as developing mature
soybean embryos by FT-IR/NIR Microspectroscopy. This is the first report of FT-NIR Chemical Images obtained with 1 micron
resolution. NIR spectra of Proteins, Oil and DNA fibers are obtained for regions as small as 1μ^2^.
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology;
Molecular Cell Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6591/version/2
Metabolic, Replication and Genomic Category of Systems in Biology, Bioinformatics and Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen
represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other
than the discrete topology of sets. Theoreticians of life’s origins postulated that Life on Earth has begun with the
simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important
question of the minimal living organism by defining the functional relations that would have made life possible in such a minimal
system- a grandad and grandma of all living organisms on Earth. Genomic systems are also considered as molecular realizations
of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics & Genomics; Molecular Cell Biology; Neuroscience; Bioinformatics;
Earth & Environment; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7051/version/1
Complex Systems Analysis of Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: I. C. Baianu.
A new approach to the modular, complex systems analysis of nonlinear dynamics in cell cycling network transformations
involved in carcinogenesis is proposed. Carcinogenesis is a complex process that involves dynamically inter-connected
biomolecules in the intercellular, membrane, cytosolic, nuclear and nucleolar compartments that form numerous inter-related
pathways referred to as networks.
The variable biotopology of such dynamic networks is highly complex,
and has a number of interesting properties that can be formally characterized at one level of organization by mathematical
structures called 'biogroupoids'. 
One such family of pathways contains the cell
cyclins. Cyclins are proteins that link several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology;
Molecular Cell Biology; Pharmacology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6205/version/1
Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for
Composition Improvements. (v.3)
Provedor de dados: 17
Autores: Valentin Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at
Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions. Conventional
“wet chemistry” analytical methods are time-consuming and costly. As a result, soybean breeders
and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive
method for composition analysis for academia and industry. Recent advancements in instrumentation design, such as the
application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly
improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell
Biology; Bioinformatics; Earth & Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/3
Molecular Models of DNA Structures and Dynamics: Principles, Techniques and Applications
Provedor de dados: 17
Autores: I. C. Baianu.
Molecular models of DNA structures are representations of the molecular geometry and topology of Deoxyribonucleic acid
(DNA) molecules using one of several means, such as: closely packed spheres (CPK models) made of plastic, metal wires for
'skeletal models', graphic computations and animations by computers, artistic rendering, and so on, with
the aim of simplifying and presenting the essential, physical and chemical, properties of DNA molecular structures either in vivo
or in vitro. Computer molecular models also allow animations and molecular dynamics simulations that are very important for
understanding how DNA functions in vivo.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Microbiology;
Molecular Cell Biology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7075/version/1
Visual Molecular Dynamics Investigations of the Impact of Hydrophobic Nanoparticles on Prognosis
of Alzheimer’s Disease and Cancers
Provedor de dados: 17
Autores: I. C. Baianu; M Charles; V. I. Prisecaru.
The possible impact of hydrophobic lectin nanoparticles on the prognosis and progression of Alzheimer's disease
(AD) and cancers was investigated by Visual Molecular Dynamics (VMD) computer modeling programs available from the
Beckmann Advanced Research Institute at the University of Illinois at Urbana. Our results indicate the possibility of impeding
pathological aggregation of certain proteins such as modified tau- or beta-amyloid that are currently being considered as possible
causes of Alzheimer's disease. VMD programs serve as useful tools for investigation hydrophobic protein aggregation
that may play a role in aging of human populations.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell
Biology; Neuroscience; Pharmacology; Bioinformatics; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7111/version/2
The Effects of Biotechnology Policy on Trade and Growth
Provedor de dados: 31
Autores: Oehmke,
James F.; Maredia,
Mywish K.; Weatherspoon,
Dave D.
Despite the clear influence of European Union biotechnology restrictions on trade patterns, very little work has been done to
model these influences or their long-run effects. This paper presents an economic trade theory model of biotechnology,
biotechnology research and development (R&D), and biotechnology regulation. The model analyzes the impact of European
Union consumer preferences on production and consumption of biotechnology products, the level and national origin of
biotechnology R&D, trade patterns, and growth. The results indicate that developing countries may become the major
producers of biotechnology agricultural products if they can establish the necessary legal infrastructure; and that European
biotechnology regulations effectively...
Tipo: Journal Article
Palavras-chave: Biotechnology; Economic growth; International trade; Regulation; Research and development; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/23872
The Production and Price Impact of Biotech Crops
Provedor de dados: 31
Autores: Brookes,
Graham; Yu,
Tun-Hsiang (Edward); Tokgoz,
Simla; Elobeid,
Amani E.
Biotech crops have now been grown commercially on a substantial global scale since 1996. This paper examines the production
effects of the technology and impacts on cereal and oilseed markets through the use of agricultural commodity models. It analyses
the impacts on global production, consumption, trade and prices in the soybean, canola and corn sectors. The analysis suggests
that world prices of corn, soybeans and canola would probably be, respectively, 5.8%, 9.6% and 3.8% higher, on average, than
2007 baseline levels if this technology was no longer available to farmers. Prices of key derivatives of soybeans (meal and oil)
would also be between 5% and 9% higher, with rapeseed meal and oil prices being about 4% higher than baseline levels. World
prices...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Cereals; Crop yields; Oilseeds; Price effects; Productivity.; Crop Production/Industries; Demand
and Price Analysis; Productivity Analysis.
Ano: 2010
URL: http://purl.umn.edu/56984
THE ECONOMICS OF BIOSAFETY: IMPLICATIONS FOR BIOTECHNOLOGY IN
DEVELOPING COUNTRIES
Provedor de dados: 31
Autores: Maredia,
Mywish K.
There is a growing body of literature on the safe use of biotechnology and the need for an international biosafety protocol and
national regulations to facilitate the safe development and transfer of biotechnology. Most of these studies, however, address the
issue of biosafety from a scientific, legal, environmental and organizational perspective. The purpose of this paper is to add to
this discussion by providing an economic perspective on regulating products of agricultural biotechnology, with special emphasis
on implications for developing countries who are under increasing pressure to put a biosafety framework in place. The paper
provides a brief discussion on the economic rationale for biosafety regulations, explains the economic benefits and costs...
Tipo: Working or Discussion Paper
Palavras-chave: Biosafety; Economic aspects; Developing countries; Agriculture; Biotechnology; Research; Research and
Development/Tech Change/Emerging Technologies.
Ano: 1998
URL: http://purl.umn.edu/11768
GM crop technology and trade restraints: economic implications for Australia and New Zealand
Provedor de dados: 31
Autores: Anderson,
Kym; Jackson,
Lee Ann.
How much might the potential economic benefit from enhanced farm productivity associated with crop biotechnology adoption by
Australia and New Zealand (ANZ) be offset by a loss of market access abroad for crops that may contain genetically modified
(GM) organisms? This paper uses the Global Trade Analysis Project (GTAP) model to estimate effects of other countries’ GM
policies without and with ANZ farmers adopting GM varieties of various grains and oilseeds. The gross economic benefits to
ANZ from adopting GM crops under a variety of scenarios could be positive even if the strict controls on imports from
GM-adopting countries by the European Union are maintained, but not if North-East Asia also applied such trade restaints. From
those gross economic...
Tipo: Article
Palavras-chave: Biotechnology; Computable general equilibrium; Genetically modified organisms; Regulation; Trade policy;
Crop Production/Industries; International Relations/Trade.
Ano: 2005
URL: http://purl.umn.edu/118502
Welfare Impacts of Cross-Country Spillovers in Agricultural Research
Provedor de dados: 31
Autores: Lence,
Sergio H.; Hayes,
Dermot J.
The welfare implications of intellectual property protection (IPP) for private sector agricultural research are analyzed, focusing on
the realistic cases in which countries provide different IPP levels, technology spills over across countries, and the public sector is
involved in research. A model is developed to determine who benefits from, and who should pay for, the associated research. The
paper contains some interesting results on the implications of a harmonization of IPP policies through multilateral agreements or
via technology that allows research firms to prevent the copying of plants and animals that express traits that have emerged from
their research.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; GURTs; Intellectual property; Research spillover; Welfare analysis; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9375
IDENTITY PRESERVATION AND LABELING OF GENETICALLY MODIFIED PRODUCTS:
SYSTEM DESIGN AND ENFORCEMENT ISSUES
Provedor de dados: 31
Autores: Moschini,
GianCarlo; Lapan,
Harvey E.
This paper analyzes economic issues that arise in devising a credible and enforceable system of identity preservation and labeling
for genetically modified (GM) and non-GM products. The model represents three stages in the supply chain: farm production,
marketing handlers, and final users. The possibility of accidental co-mingling of non-GM products is modeled at the marketing
stage. Regulation takes the form of a threshold level of purity for non-GM products, a probability of government testing to verify
compliance with the threshold level, and a fine for violators. Uncertainty is modeled explicitly, such that would-be suppliers of
non-GM products always face some risk of failing the test and incurring a fine. The paper also presents a novel demand...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Enforcement; Food labeling; Identity preservation; Regulation; Uncertainty; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/18355
Who Benefits from Quality Labelling? Segregation Costs, International Trade and Producer
Outcomes
Provedor de dados: 31
Autores: Jackson,
Lee Ann.
This paper analyses the impact of quality based labelling on product prices, factor allocation and the resulting effects on producers
within the context of an international trading system. A general equilibrium model, calibrated to 1998 data, describes United
States and European Union labelling regimes for genetically modified agricultural products. The results indicate that the labelling
choice of trade partners have large distributive impacts within national economies, as well as across countries and highlight the
importance of using general equilibrium framework to understand the system wide impacts of labelling policies that differentiate
products based upon quality characteristics. These results are essential for policy makers seeking to understand...
Tipo: Conference Paper or Presentation
Palavras-chave: Labelling; International trade; Political economy; General equilibrium; Biotechnology.; International
Relations/Trade; Marketing; Political Economy.
Ano: 2003
URL: http://purl.umn.edu/57897
VALUATION OF INTERNATIONAL PATENT RIGHTS FOR AGRICULTURAL
BIOTECHNOLOGY: A REAL OPTIONS APPROACH
Provedor de dados: 31
Autores: Nadolnyak,
Denis A.; Sheldon,
Ian M.
Uncertainty of returns from marketing is an extremely important factor affecting the diffusion of a wide range of genetically
modified (GM) crops worldwide. Biotech companies face complicated choices in making decisions about whether to enter
agricultural markets in different countries with their agricultural products. In this paper, we model these choices as a real option
problem of entry decision solved at a micro-level by individual firms. The model is aggregated in order to reflect the
heterogeneity of different genetic events, as well as different markets, in terms of their profitability. The solution to the model
produces distributions of entry probabilities as functions of the functional forms (and parameter values) that govern the evolution
of...
Tipo: Conference Paper or Presentation
Palavras-chave: Patent values; Agricultural biotechnology; Real options; Numerical simulation.; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/21982
VALUATION OF BIOTECHNOLOGY COMPANIES: REAL OPTIONS APPROACH UNDER
UNCERTAINTY
Provedor de dados: 31
Autores: Ljumovic,
Isidora; Cvijanovic,
Janko; Lazic,
Jelena.
Review article
Tipo: Article
Palavras-chave: Risk; Real options; Valuation; Biotechnology; Agribusiness; Risk and Uncertainty; D04; D81; L65.
Ano: 2012
URL: http://purl.umn.edu/123958
The Emergence of Insect Resistance in Bt-Corn: Implication of Resistance Management Information
Under Uncertainty
Provedor de dados: 31
Autores: Linacre,
Nicholas A.; Thompson,
Colin J.
The successful management of transgenic technology is likely to depend on the economic behavioral response of farmers to the
regulated use of transgenic crops. A well-studied example is the widespread use of Bt-corn, in the United States, and elsewhere, to
control the European Corn Borer, a major corn pest. The extensive use of Bt-corn has led to concerns about the emergence of
insect resistance. The United States Environment Protection Agency addressed this potential problem by developing an insect
resistance management strategy, based, in part, on complex mathematical models using detailed biological assumptions about the
population genetics and life history of the European Corn Borer. However, seed companies and others have sometimes used
simpler...
Tipo: Working or Discussion Paper
Palavras-chave: Insect resistance; Monte Carlo; Bt-corn; Logistic growth; Environmental risk; Biotechnology; Environmental
protection; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/59239
AGRICULTURAL BIOTECHNOLOGY: FARM-LEVEL, MARKET, AND POLICY
CONSIDERATIONS
Provedor de dados: 31
Autores: Van der Sluis,
Evert; Diersen,
Matthew A.; Dobbs,
Thomas L.
This study provides an overview of the economic costs, benefits, and risks involved with agricultural biotechnology at the farm
level, at the market level, and for the farm and food system as a whole. Both advantages and disadvantages of agricultural
biotechnology are discussed. Among the drivers of U.S. domestic and international consumer demand for transgenic crop products
discussed are environmental and food safety concerns. A comparison is made between a "science-based" regulatory framework
and a policy based on the precautionary principle. The authors argue that open dialogue is needed for achieving improved public
understanding of agricultural biotechnology, and that analyses need to go beyond discussing the scientific merits of
biotechnology, to...
Tipo: Journal Article
Palavras-chave: Acceptance; Agricultural biotechnology; Benefits; Costs; Public dialogue; Risks; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/14654
Parables: applied economics literature about the impact of genetically engineered crop varieties in
developing economies
Provedor de dados: 31
Autores: Smale,
Melinda; Zambrano,
Patricia; Falck-Zepeda,
Jose Benjamin; Gruere,
Guillaume P.
A vast literature has accumulated since crop varieties with transgenic resistance to insects and herbicide tolerance were released to
farmers in 1996 and 1997. A comparatively minor segment of this literature consists of studies conducted by agricultural
economists to measure the farm-level impact of transgenic crop varieties, the size and distribution of the economic benefits from
adopting them, consumer attitudes toward GE products, and implications for international trade. This paper focuses only on the
applied economics literature about the impact of transgenic crop varieties in non-industrialized agricultural systems, with an
emphasis on methods. A number of studies have surveyed the findings for both industrialized and non-industrialized agriculture,...
Tipo: Report
Palavras-chave: Genetically engineered crops; Economic impacts; Technology adoption; Developing economies; Economics
methods; Best practices; Biotechnology; Crop Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55412
An analysis of trade related international regulations of genetically modified food and their effects on
developing countries
Provedor de dados: 31
Autores: Gruere,
Guillaume P.
This paper reviews current trade-related regulations of genetically modified (GM) food and discusses their effects on developing
countries. There is a large heterogeneity in current import approval and marketing policies of GM food worldwide. At the
international level, the harmonization efforts are led by the Codex Alimentarius Commission, the Cartagena Protocol on Biosafety
and the World Trade Organization. While internationally harmonized guidelines for safety approval have been finalized, we show
that there is no clear consensus on labeling regulations for GM food, and there is an increasing risk of conflicts among
international agreements. We analyze the GM food regulations of two large rich importers, Japan and the European Union (EU)
and discuss...
Tipo: Report
Palavras-chave: Genetically modified food; Developing countries; Biotechnology; Biosafety; Trade regulation; Labeling;
International trade; Tariff; International Relations/Trade.
Ano: 2006
URL: http://purl.umn.edu/55422
AN INITIAL LOOK AT THE TOKYO GRAIN EXCHANGE NON-GMO SOYBEAN CONTRACT
Provedor de dados: 31
Autores: Parcell,
Joseph L.
Genetically modified organisms (GMOs) such as soybeans and corn have garnered considerable consumer attention due to the
concern over potential effects from using these commodities as inputs into food production. In the simplest form, segregation of
bioengineered and non-bioengineered crops is an identity-preserved system. For these identity-preserved systems to exist, there
must be a market discovery mechanism in place whereby supply and demand factors interact to establish a market price. In May
2000, the Tokyo Grain Exchange began a nongenetically modified organism (non-GMO) soybean contract. This article describes
information garnered from the first public-offered identity-preserved marketplace.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Identity-preserved markets; Non-GMO; Soybean contract;
Tokyo Grain Exchange; Marketing.
Ano: 2001
URL: http://purl.umn.edu/14684
THE SOURCE OF COMPARATIVE ADVANTAGE IN THE BIOTECHNOLOGY INDUSTRY: A
REAL OPTIONS APPROACH
Provedor de dados: 31
Autores: Lavoie,
Brian F.; Sheldon,
Ian M.
Sources of heterogeneity within the process of R&D investment, such as international differences in the maximum per-period
rate of investment and level of regulatory uncertainty, offer a plausible explanation for US comparative advantage in
biotechnology. Using dynamic stochastic simulation, the results presented in this paper suggest US biotechnology firms may
initiate more R&D projects, innovate earlier and more rapidly, persevere longer in the face of mounting R&D costs, and
successfully complete more R&D projects than European firms.
Tipo: Conference Paper or Presentation
Palavras-chave: Comparative advantage; Biotechnology; Real options; Industrial Organization; Research and Development/Tech
Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21508
An Analysis of McLean County, Illinois Farmers' Perceptions of Genetically Modified Crops
Provedor de dados: 31
Autores: Chimmiri,
Nagesh; Tudor,
Kerry W.; Spaulding,
Aslihan D.
McLean County, Illinois farmers were surveyed in order to explore and analyze their perceptions of genetically modified crops
and their genetically modified cropping decisions. Questionnaires were mailed to 400 randomly selected farmers, and 156 were
returned. The 134 respondents who reported that they planned to plant crops in 2003 were asked to provide information about
gender, age, education, and number of tillable acres farmed. Respondents were also asked if they had previously planted
genetically modified crops and if they planned to plant either genetically modified corn or genetically modified soybeans in 2003.
Finally, respondents were presented with 40 statements that pertained to biotechnology and genetically modified crops, and they
were asked...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Genetically modified; Corn; Soybeans; Research and Development/Tech Change/Emerging
Technologies; Q16.
Ano: 2005
URL: http://purl.umn.edu/19204
Public Policy and Endogenous Beliefs: The Case of Genetically Modified Food
Provedor de dados: 31
Autores: Lusk,
Jayson L.; Rozan,
Anne.
When individuals have limited information and are uncertain about the quality of a good, government policy, or the lack thereof,
can serve as a signal to consumers about the likelihood of realizing alternatives states of nature. In this paper, we focus on a
controversial beliefs about government intervention: the market for genetically modified food. Data from a mail survey were used
to estimate an econometric model where beliefs about labeling policy, beliefs about the safety of genetically modified food, and
willingness to consume genetically modified food are endogenously determined. Results indicate that consumers who believe the
government has a mandatory labeling policy for genetically modified food are more likely to believe genetically modified...
Tipo: Journal Article
Palavras-chave: Biotechnology; Food labeling policy; Genetically modified food; Trivariate probit; Demand and Price Analysis;
Food Consumption/Nutrition/Food Safety.
Ano: 2008
URL: http://purl.umn.edu/42460
Incorporating Collateral Information Using an Adaptive Management Framework for the Regulation
of Transgenic Crops
Provedor de dados: 31
Autores: Linacre,
Nicholas A.; Burgman,
Mark A.; Ades,
Peter K.; Stewart-Oaten,
Allen.
A lack of data often makes biological management decisions difficult and has been an area of contention in the debate over the
approval of transgenic crops. Our knowledge of natural systems is limited and our ability to gain additional information, quickly
and effectively, is often handicapped by statistical complexity. To adequately cope with this requires new approaches and models
that integrate decision-making and management. This paper describes one possible approach to the integration of decision-making
and management, which may have application for the regulatory approval of transgenic crops. In many situations countries
wishing to approve transgenic crops will have limited data on the environmental performance of the crop. The approach outlined
in...
Tipo: Working or Discussion Paper
Palavras-chave: Bayesian estimation; Collateral data; Inference; GMO; Transgenic plants; Bayesian theory; Biotechnology;
Biosafety; Regulatory systems; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/59233
Student Response to Transgenic Meat: An analysis of a Fort Valley State University Survey
Provedor de dados: 31
Autores: Ibrahim,
Mohammed; Brewer,
Alex; Johnson,
Filton; Whitehead,
Jackie.
The study examines the factors the factors that influence Fort Valley State University students’ willing to purchase transgenic
meat. Results show that respondents who said they read labels when shopping were more likely to purchase transgenic meat.
Results also suggest that respondents who trust scientists to tell them the truth about transgenic meat were more likely to purchase
transgenic meat. Respondents who identified themselves as sophomores, however, were less likely to consume transgenic meat.
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic meat; Willingness to accept; Biotechnology; Genetically modified organisms; Agribusiness;
Agricultural and Food Policy; Consumer/Household Economics.
Ano: 2010
URL: http://purl.umn.edu/56766
Factors Affecting Biotechnology Innovation in Canada: Analysis of the 2001 Biotechnology Use and
Development Survey
Provedor de dados: 31
Autores: van Moorsel,
Daryl; Cranfield,
John A.L.; Sparling,
David.
Advancement in biotechnology requires continued innovative activity by firms. To grow, biotechnology firms must understand
the factors affecting their innovative activity. Such understanding also informs policy makers, and supports the development of
policies promoting one's biotechnology sector. This study explores factors which determine innovative activity within the
Canadian biotechnology industry. Innovative activity is measured as the natural log of the number of products/processes a firm
has at different stages of the innovation spectrum. A model is developed to regress this measure on several determinants of
innovation. Significant drivers of innovation include: collaborative arrangements, transfer of intellectual property, firm size and
age,...
Tipo: Working or Discussion Paper
Palavras-chave: Innovation; Biotechnology; Canada; Agriculture; Food; Human health; Research and Development/Tech
Change/Emerging Technologies.
Ano: 2005
URL: http://purl.umn.edu/34121
Consequences of Biotechnology Policy for Competitiveness and Trade of Southern U.S. Agriculture
Provedor de dados: 31
Autores: Jolly,
Curtis M.; Jefferson-Moore,
Kenrett Y.; Traxler,
Greg.
The effect of policy decisions on the competitiveness of genetically modified (GM) crops was examined. The United States has
been an early innovator in the development and use of biotechnology crops and has expanded its export market share of the three
major GM crops: soybeans, cotton, and corn. Cotton, soybeans, and corn are all grown in the southern states, but these states
have an apparent comparative advantage only in the production of cotton, which may be strengthened with the adoption of
genetically modified cotton. The influence of biotechnology on the competitiveness of soybeans and corn for the southern states
through the introduction of genetically modified organisms (GMOs) is not clear but is probably negligible.
Tipo: Journal Article
Palavras-chave: Biotechnology; Competitiveness; Southern agriculture; Q13; Q17; Q16; Q18.
Ano: 2005
URL: http://purl.umn.edu/43514
BIOTECHNOLOGY REGULATIONS AND THE WTO
Provedor de dados: 31
Autores: Sheldon,
Ian M.; Josling,
Timothy E.
This paper examines the regulation of trade in genetically modified organisms (GMOs) by the World Trade Organization (WTO).
Despite rapid adoption of GMOs by a few exporters, many importers have developed relatively restrictive procedures for
pre-market approval of GMOs, and are introducing mandatory labeling. While exporters have yet to seek a ruling from the WTO
on these regulations, a trade dispute over GMOs is likely to occur before too long. Exporting countries will likely argue that
importing countries' regulations are too restrictive, given existing scientific knowledge of the safety of current GM crops, and that
labeling of GM foods is unnecessary due to the fact that they are typically similar to their conventional counterparts. In
response,...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Regulation; Trade; WTO; International Relations/Trade; Research and Development/Tech
Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/14594
EMPLOYMENT IN BIOTECHNOLOGY IN GERMANY
Provedor de dados: 31
Autores: Menrad,
Klaus.
Paper prepared for presentation at the 8th ICABR International Conference on Agricultural Biotechnology: International Trade
and Domestic Production Ravello (Italy), July 8 to 11th, 2004
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Employment; Germany; Application industries; Simulation; Labor and Human Capital; E24;
J21.
Ano: 2004
URL: http://purl.umn.edu/91012
MARKETING STRATEGIES OF BIOTECHNOLOGY FIRMS: IMPLICATIONS FOR U.S.
AGRICULTURE
Provedor de dados: 31
Autores: Renkoski,
Matthew A.
DuPont Quality Grains is focused on improving grain quality for end users, rather than on farm production traits. A major
DuPont program is high oil corn. Feed corn has a huge market, and, because it requires no intermediate processing, any
enhanced value is measurable by end users. Standard commodity markets do not function for capturing the value of enhance
grains, and so DuPont works with end users, elevators, farmers, and seed companies to create market channels. As biotechnology
commercializes more value-enhancing traits targeted to specific agricultural customers, vertical value chains will become shorter
and more coordinated while standard commodity markets will diminish.
Tipo: Journal Article
Palavras-chave: Biotechnology; DuPont; Marketing strategies; Research and Development/Tech Change/Emerging
Technologies.
Ano: 1997
URL: http://purl.umn.edu/15550
CONCENTRATION AND TECHNOLOGY IN AGRICULTURAL INPUT INDUSTRIES
Provedor de dados: 31
Autores: King,
John L.
Consolidation in the agricultural biotechnology industry can both enhance and dampen market competition. This report examines
the causes and consequences of industry consolidation and its effect on market efficiency. In some cases, concentration realizes
economies of scale, which can improve market efficiency by driving down production costs. The protection of intellectual
property rights is integral to the agricultural biotechnology marketplace, stimulating research and development, investment, and
the development of substitute markets. However, excessively broad intellectual property rights can hinder the market for
innovation. Recent data on mergers, acquisitions, and strategic collaborations in the agricultural biotechnology industry, as well as
the...
Tipo: Report
Palavras-chave: Industry concentration; Consolidation; Biotechnology; Market efficiency; Market power; Intellectual property
rights; Agricultural input industries; Mergers; Acquisitions; Agribusiness; Industrial Organization.
Ano: 2001
URL: http://purl.umn.edu/33631
OFF-FARM WORK AND THE ADOPTION OF HERBICIDE-TOLERANT SOYBEANS
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge; Hendricks,
Chad.
Adoption of herbicide-tolerant (HT) soybeans is proceeding rapidly despite little difference between the net returns to HT versus
conventional soybeans. Using a multivariate probit model, we analyze the interaction of off-farm work and adoption and test if
farmers adopt HT soybeans because its simplicity and flexibility frees management time.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Herbicide-tolerant soybeans; Technology adoption; Off-farm work; Household models; Crop
Production/Industries.
Ano: 2003
URL: http://purl.umn.edu/35133
PRODUCTION SYSTEMS COMPETITION AND THE PRICING OF INNOVATIONS: AN
APPLICATION TO BIOTECHNOLOGY AND SEED CORN
Provedor de dados: 31
Autores: Alexander,
Corinne E.; Goodhue,
Rachael E.
Farmers now choose production systems rather than individual inputs. This change may limit biotechnology innovation rents.
We model a corn farmer's production choice among four systems: traditional, herbicide-resistant, insect resistant, and high-oil.
We compare actual seed and chemical prices to the maximum rent the biotechnology firm can obtain.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Corn; Crop Production/Industries; Research and Development/Tech Change/Emerging
Technologies.
Ano: 1999
URL: http://purl.umn.edu/21646
THE ECONOMICS OF INTELLECTUAL PROPERTY RIGHTS UNDER IMPERFECT
ENFORCEMENT: DEVELOPING COUNTRIES, BIOTECHNOLOGY AND THE TRIPS
AGREEMENT
Provedor de dados: 31
Autores: Giannakas,
Konstantinos.
This paper develops a model of heterogeneous producers to examine the economic causes of IPR infringement and its
consequences for the welfare of the interest groups and the pricing and adoption of a new technology (i.e., a genetically modified
seed) in the context of a small open developing economy. Enforcement of IPRs, and pricing and adoption of the new technology
are modeled as a sequential game between the government that enforces the IPRs, a foreign innovating firm that prices the new
technology, and the developing country’s producers who make the production and cheating decisions. Analytical results show that
complete deterrence of IPR infringement is not always economically optimal. IPR infringement affects the welfare of the interest
groups and...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Enforcement; Infringement; Intellectual property rights; TRIPs agreement; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/16063
Betting on cotton: Potential payoffs and economic risks of adopting transgenic cotton in West Africa
Provedor de dados: 31
Autores: Falck-Zepeda,
Jose Benjamin; Horna,
J. Daniela; Smale,
Melinda.
Cotton is the largest source of export receipts in several West African nations where yields are declining and pesticide use is
rising. Although there may be payoffs to introducing genetically modified Bt (Bacillus thurigiensis) cotton, limited information is
available to predict its potential economic impact and there is uncertainty about its performance. Recognizing these constraints,
we use an economic surplus model augmented with stochastic simulation to estimate ex ante the impact and distribution of
benefits from Bt cotton. We consider the effects of adoption on both yields and abating crop damage, and offer scenarios depicting
the policy options faced by West African stakeholders. The findings indicate that although the total net benefits of...
Tipo: Journal Article
Palavras-chave: Crop biotechnology; Bt cotton; Economic surplus model; West Africa; Agricultural development; Risk; Crop
Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/56962
DOA-Maize research and development
Provedor de dados: 106
Autores: Pichet Grudloyma; Somrak Noradechanon; Surapong Prasitwattanaseree; Kalaya papintu; Manoon Pumklom.
9 tables
Palavras-chave: Corn; Maize; Population improvement; Hybrids; Cooperative program; Biotechnology; AMBIONET; Plant
breeding; ข้าวโพด; การปรับปรุ งประชากร; พันธุ์ลูกผสม; โครงการความร่ วมมือ; เทคโนโลยีชีวภาพ; แอมไบโอเนท.
Ano: 2001
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4159
Pesquisa, desenvolvimento e inovação para o Cerrado.
Provedor de dados: 119
Autores: FALEIRO, F. G.; SOUSA, E. dos S. de (Ed.).
Contribuição tecnológica da Embrapa Cerrados para a agropecuária no bioma Cerrado; Caracterização e conservação da
biodiversidade do bioma Cerrado; Gestão de recursos hídrico e manejo de irrigação no Cerrado; Manejo e fertilidade de solo no
Cerrado: contribuição da pesquisa; Viabilidade do cultivo do trigo no Cerrado do Brasil Central; Cultivares de trigo para o
Cerrado; Cevada: um exemplo de cultura alternativa para o sistema irrigado do Cerrado; Manejo estratégico da irrigação e
aspectos fitotécnicos do cafeeiro no Cerrado; Diversificação da produção agrícola no Cerrado; Integração lavoura-pecuária na
região do Cerrado; Uso do enfoque de P&D para apoiar o desenvolvimento da agricultura familiar: a experiência dos projetos
Silvânia e Unaí; Zoneamento...
Tipo: Livros científicos (ALICE)
Palavras-chave: Cerrado; Pesquisa; Conhecimento; Tecnologia; Biodiversidade; Recurso hídrico; Fertilidade do solo;
Agricultura familiar; Biotecnologia; Research; Knowledge; Technology; Biodiverstiy; Water resources; Soil fertility; Family
farms; Biotechnology.
Ano: 2007
URL: http://www.alice.cnptia.embrapa.br/handle/doc/571723
Plant antimicrobial peptides: an overview of superSAGE transcriptional profile and a functional
review.
Provedor de dados: 119
Autores: KIDO, E. A.; PANDOLFI, V.; HOULLOU-KIDO, L. M.; ANDRADE, F. C.; MARCELINO, F. C.; NEPOMUCENO,
A. L.; ABDELNOOR, R. V.; BURNQUIST, W. L.; BENKO-ISEPPON, A. M.
Defensin, thionin and lipid transfer protein (LTP) gene families, which antimicrobial activity has an attractive use in protein
engineering and transgenic production of agronomically important plants, have been here functionally reviewed. Also, a
transcriptional overview of plant SuperSAGE libraries and analysis of 26 bp tags possibly annotated for those families are
presented. Tags differentially expressed (p < 0.05) or constitutively transcribed were identified from leaves or roots from
SuperSAGE libraries from important Brazilian plant crops [cowpea (Vigna unguiculata (L.) Walp.), soybean (Glycine max (L.)
Merr.) and modern sugarcane hybrids (Saccharum spp.)] submitted to abiotic [salt (100 mM NaCl) or drought] or biotic stresses
[fungus inoculation...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Expressão gênica; PR proteins; Defensin; Thionin; Lipid transfer protein; Stress; Biotecnologia; Soja; Feijão;
Cana de açúcar; Genoma; Soybeans; Agricultural biotechnology; Genomics; Cowpeas; Sugarcane.
Ano: 2010
URL: http://www.alice.cnptia.embrapa.br/handle/doc/875446
Uso de marcadores RAPD e do sistema de informação geográfica no estudo da variabilidade genética
e ecológica de Stylosanthes macrocephala M. B. Ferr. et S. Costa.
Provedor de dados: 119
Autores: COSTA, A. M.
As espécies do gênero Stylosanthes Sw. vêm sendo utilizadas em muitos países na recuperação de áreas degradadas, na adubação
verde e na alimentação animal. A. S. macrocephala M. B. Ferr. et. S Costa tem despertado o interesse de pesquisadores que
realizam trabalhos de conservação e melhoramento genético devido ao seu potencial produtivo, resistência a seca e a antracnose, e
grande produção de semente a falta de descritores morfológicos e agronômicos estáveis e de informações ecogeograficas dos
locais de coleta dos acessos tem a caracterização, o estudo da variabilidade genética, e cosequentemente a utilização dos mesmos
em programas de melhoramento genético. Com o objetivo de reduzir tais dificuldades, neste trabalho foram obtidos os descritores
ecológicos...
Tipo: Teses/dissertações (ALICE)
Palavras-chave: Biotecnologia; Marcador molecular; RAPD; Stilosante; Leguminosa forrageira; Melhoramento genético vegetal;
Variação genética; Biotechnology; Genetic markers; Feed legumes; Plant breeding; Genetic variation; Geographic information
systems.
Ano: 2004
URL: http://www.alice.cnptia.embrapa.br/handle/doc/568065
Efeito da Benzilaminopurina (Bap) na micropropagação da variedade curimenzinha (Bgm 611) de
mandioca (Manihot esculenta Crantz).
Provedor de dados: 119
Autores: CARVALHO, M. de J, da S. de; SOUZA, A. da S.; SANTOS, K. C. F. dos; MENDES, M. I. de S.; OLIVEIRA, E. J. de.
A mandioca pode ser afetada por problemas que limitam ou inviabilizam seu desenvolvimento. Dentre os fatores que afetam essa
cultura destacam-se os bióticos, como as podridões de raízes, a bacteriose e os ácaros, e os abióticos, especialmente a seca. Outro
fator que interfere de forma marcante na produção de mandioca é a falta de material de plantio de qualidade genética e
fitossanitária, já que seu sistema de propagação vegetativo, além da baixa taxa que apresenta, favorece o acúmulo e a transmissão
de pragas e patógenos entre os ciclos de cultivo. Dessa forma, é fundamental o fornecimento de mudas sadias visando contornar
essa situação. Nesse sentido, a biotecnologia surge como uma alternativa por meio da multiplicação in vitro de materiais livres
de...
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Mandioca; Reprodução vegetal; Biotecnologia; Cultura in vitro; Cultura de tecido; Hormônio vegetal;
Fitohormonio; Cassava; Plant breeding; Micropropagation; Biotechnology; In vitro culture; Tissue culture; Plant hormones;
Phytohormones.
Ano: 2013
Identification of reference genes for expression analysis by real-time quantitative PCR in
drought-stressed soybean.
Provedor de dados: 119
Autores: STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.;
PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies
of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin,
GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system
with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand
with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the
efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm
application was used to...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Estabilidade de expressão; Expressão gênica; RT-qPCR; Soja; Biotecnologia; Deficiência hídrica; Soybeans;
Gene expression; Plant-water relations; Agricultural biotechnology.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/handle/doc/882641
Hegemon??as culturales e impertinencias tecnol??gicas : reflexiones en torno a la potencial
introducci??n de transg??nicos en el agro ecuatoriano (Debate Agrario-Rural)
Provedor de dados: 122
Autores: Cuvi, Nicol??s.
El debate sobre el uso e investigaci??n de organismos gen??ticamente modificados (transg??nicos) fue reabierto a nivel nacional
por el gobierno ecuatoriano en septiembre de 2012. Con base en ejemplos hist??ricos del pa??s, y del an??lisis de opiniones a
favor y en contra del uso de transg??nicos, en este art??culo se reflexiona c??mo la introducci??n de estos organismos perpetuar??
un secular modelo de apuesta por modos de producir que acent??an las inequidades e inseguridades nacionales, y generar??
impactos negativos, de ??ndole tecnol??gica, social, econ??mica y ambiental, en las zonas rurales. Se propone la agroecolog??a
como alternativa para un desarrollo tecnol??gico pertinente.
Tipo: Art??culo
Palavras-chave: TRANSG??NICOS; MEDIO AMBIENTE; AGRICULTURA; BIOTECNOLOG??A; AGROECOLOG??A;
TRANSGENIC; ENVIRONMENT; AGRICULTURE; BIOTECHNOLOGY; AGROECOLOGY; ECUADOR.
Ano: 2013
URL: http://hdl.handle.net/10469/5408
Produção de material vegetativo de videira livre de virus no submédio São Francisco.
Provedor de dados: 14
Autores: LEAO, P. C. de S.; MELO, N. F. de; PIRES, E. da L.
Tem como objetivo produzir material vegetativo livre de virus das principais cultivares de videira de interesse comercial para o
submedio Sao Francisco, proporcionando aos produtores e viveiristas da Regiao Nordeste do Brasil a possibilidade de disporem
de mudas certificadas sadias e de elevada qualidade, evitando a disseminacao de viroses e, com isto, permitindo a implantacao de
vinhedos homogeneos e de elevada produtividade.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Uva; Videira; Muda; Doenca; Virus; Resistencia; Biotecnologia; Meristema; Micropropagacao; Brasil;
Submedio Sao Francisco; Grape; Seedlings; Diseases; Resistance; Biotechnology; Meristems; Micropropagation; Brazil; Uva;
Grape.
Ano: 1997
Plantas transgênicas e a microbiota do solo.
Provedor de dados: 14
Autores: REIS JÚNIOR, F. B. dos; MENDES, I. de C.; HUNGRIA, M.
ABSTRACT: Soil micro-organisms represent a key hole in the nutrient cycle and in the maintenance of the fertility, besides
execute another variety of important functions for the plants and the environment. With the possibility of the transgenic crops to
cause negative impacts on the soil microbial communities, emerge the necessity to deep evaluate the real extensions of these
impacts. In such case, this document presents some discussions about the interactions between transgenic plants and soil
micro-organisms, showing results from different studies conducted by many reseachers in the world and focusing in the evaluated
parameters and the methodologies utilised to analyse the microbial communities.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Microbiologia do solo; População microbiana; Planta transgênica; Doença de planta; Praga de planta; Herbicida;
Resistência; Biotecnologia; Soil biology; Microbial flora; Transgenic plants; Plant diseases; Pests of plants; Herbicides;
Resistance; Biotechnology.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/569840
Transformação de plantas.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods
of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant
breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain new varieties. Genetic
transformation is the targed organism using non sexual methods. Transgenic plants can be defined as those the present genes
different from the original genoma obtained by techniques of genetic engineering. The transformation stages consist of the
identification isolation, cloning and introduction of gene into the cells. There are several transformation methods, and the choice...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Melhoramento genético vegetal; Biotecnologia; Planta transgênica; Transformação genética; Bactéria;
Agrobacterium; Gene; DNA; Biolística; Eletroporação; Plant breeding; Biotechnology; Transgenic plants; Genetic transformation;
Genes; Biolistic; Eletroporation.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/898842
Metodologia para operacionalizar a extracao de DNA de especies nativas do cerrado visando a
analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
2003
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Marcador molecular; DNA; Biotecnologia; Melhoramento genetico vegetal; Marcador genetico; Engenharia
genetica; Biologia molecular; Cerrado; Biotechnology; Plant breeding; Genetic markers; Genetic engineering; Molecular biology.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/559088
Engenharia genética: conceitos básicos, ferramentas e aplicações.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to
people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the
importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic
research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication,
transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid
libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Engenharia genética; Biotecnologia; Marcador genético; DNA; RNA; Ácido nucléico; Gene; Biologia
molecular; Clonagem; Transgênico; Genetic engineering; Biotechnology; Genetic markers; Nucleic acids; Genes; Molecular
biology; Cloning; Transgenic.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/568132
Genetic variability of mango (Mangifera indica L.) cultivars used in the Embrapa Cerrados breeding
program using RAPD markers.
Provedor de dados: 14
Autores: FALEIRO, F. G.; PINTO, A. C. Q.; CORDEIRO, M. C. R.; RAMOS, V. H. V.; BELLON, G.; ANDRADE, S. R. M.;
PINTO, J. F. N.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; Melhoramento genetico; Manga; Mangifera indica; Metodo de melhoramento; Variacao
genetica; RAPD; Marcador genetico; Biotecnologia; Cerrado; Genetic markers; Plant breeding; Mango; Breeding methods;
Genetic variation; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/566512
Diversidade genetica de especies silvestres de maracujazeiro com resistencia multipla a doencas com
base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BELON, G.; BORGES, T. A.; ANJOS, J. de R. N. dos; PEIXOTO, J. R.;
BRAGA, M. F.; SANTOS, D. G.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; RAPD; Marcador genetico; Maracuja; Passiflora edulis; Biotecnologia; Variedade
resistente; Doenca de planta; Variacao genetica; Genetic markers; Passion fruits; Disease resistance; Plant diseases; Genetic
variation; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567929
Diversidade genetica de uma colecao de trabalho de Stylosanthes guianensis com base em marcadores
RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; KARIA, C. T.; ANDRADE, R. P.; BARROS, A. M.; SILVA, D. de O. C. e.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; Variacao genetica; Marcador genetico; RAPD; Leguminosa forrageira; Melhoramento
genetico vegetal; Genetic markers; Genetic variation; Feed legumes; Plant breeding; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567917
Utilizacao de marcadores moleculares em retrocruzamentos visando a resistencia do
maracujazeiro-azedo a multiplas doencas.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BELON, G.; KRALH, L. L.; ANJOS, J. de R. N. dos; PEIXOTO, J. R.;
BRAGA, M. F.; REZENDE, A. M.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; Maracuja; Passiflora edulis; Marcador genetico; Melhoramento genetico; Doenca de
planta; Retrocruzamento; Variedade resistente; Biotecnologia; Genetic markers; Plant breeding; Passion fruits; Disease resistance;
Plant diseases; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/566509
Variabilidade genética de Mahanarva spectabilis (Distant, 1909) coletada em brachiaria brizantha cv.
marandu no estado de Tocantins, com base em marcadores RAPD.
Provedor de dados: 14
Autores: PAULA-MORAES, S. V. de; FALEIRO, F. G.; VILELA, M. de F.; JUNQUEIRA, K. P.; BELLON, G.; CARVALHO,
G. S.; AUAD, A. M.; TAKADA, S.; PONTES, R. A.; GONÇALVES NETO, S. de C.
2006
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Variação genética; Biotecnologia; Marcador genético; Marcador molecular; Praga de planta; Cigarrinha;
Gramínea forrageira; Capim brachiaria; Genetic variation; Biotechnology; Genetic markers; Pests of plants; Feed grasses.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/570176
Transformação de plantas.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods
of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant
breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain new varieties. Genetic
transformation is the targed organism using non sexual methods. Transgenic plants can be defined as those the present genes
different from the original genoma obtained by techniques of genetic engineering. The transformation stages consist of the
identification isolation, cloning and introduction of gene into the cells. There are several transformation methods, and the choice...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Melhoramento genético vegetal; Biotecnologia; Planta transgênica; Transformação genética; Bactéria;
Agrobacterium; Gene; DNA; Biolística; Eletroporação; Plant breeding; Biotechnology; Transgenic plants; Genetic transformation;
Genes; Biolistic; Eletroporation.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/562707
Oceans and Human Health: A Rising Tide of Challenges and Opportunities for Europe
Provedor de dados: 5
Autores: Fleming, L.e.; Mcdonough, N.; Austen, M.; Mee, L.; Moore, Michael; Hess, Philipp; Depledge, M.h.; White, M.;
Philippart, Katja; Bradbrook, P.; Smalley, A.
The European Marine Board recently published a position paper on linking oceans and human health as a strategic research
priority for Europe. With this position paper as a reference, the March 2014 Cornwall Oceans and Human Health Workshop
brought together key scientists, policy makers, funders, business, and non governmental organisations from Europe and the US to
review the recent interdisciplinary and cutting edge research in oceans and human health specifically the growing evidence of the
impacts of oceans and seas on human health and wellbeing (and the effects of humans on the oceans). These impacts are a
complex mixture of negative influences (e.g. from climate change and extreme weather to harmful algal blooms and chemical
pollution) and beneficial...
Tipo: Text
Palavras-chave: Harmful algal blooms; Microbial pollution; Anthropogenic chemicals; Marine biotechnology; Climate change;
Ocean acidification; Fisheries; Aquaculture; Seafood; Blue carbon; Marine energy; Blue gym; Ocean literacy; Risks; Benefits; EU
Policy.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00192/30328/29066.pdf
Production of a chimeric enzyme tool associating the Trichoderma reesei swollenin with the
Aspergillus niger feruloyl esterase A for release of ferulic acid
Provedor de dados: 189
Autores: LEVASSEUR, A.; Saloheimo, M.; NAVARRO, D.; Andberg, M.; Monot, F.; Nakari-Setälä, T.; ASTHER, M.;
RECORD, E.
The main goals of this work were to produce the fusion protein of the Trichoderma reesei swollenin I (SWOI) and Aspergillus
niger feruloyl esterase A (FAEA) and to study the effect of the physical association of the fusion partners on the efficiency of the
enzyme. The fusion protein was produced up to 25 mg l−1 in the T. reesei strains Rut-C30 and CL847. In parallel, FAEA alone
was produced for use as a control protein in application tests. Recombinant FAEA and SWOI–FAEA were purified to
homogeneity and characterized. The biochemical and kinetic characteristics of the two recombinant proteins were found to be
similar to those of native FAEA, except for the temperature stability and specific activity of the SWOI–FAEA. Finally, the
SWOI–FAEA protein was...
Tipo: Journal Article
Palavras-chave: ACIDE FERULIQUE; ACIDE COUMARIQUE; SON DE BLE CARBOXYLIC ESTER HYDROLASE;
RECOMBINANT FUSION PROTEIN; WHEAT BRAN; FERULOYL ESTERASE; CHIMERIC PROTEIN; SWOLLENIN;
BIOTECHNOLOGY; FUNGUS.
Ano: 2006
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200724ba03ab&uri=/notices/prodinra1/2010/09/
Les cultures transgéniques tolérantes à un herbicide permettent-elles de réduire l’usage des pesticides
? Le cas du soja et du maïs aux Etats-Unis
Provedor de dados: 189
Autores: Bonny, S.
Les cultures transgéniques tolérantes à un herbicide (TH), actuellement largement prédominantes parmi les OGM, ont fait l’objet
de nombreuses critiques notamment en raison de leur emploi concomitant d’herbicides. Mais celui-ci est-il similaire, plus ou
moins élevé qu’en cultures conventionnelles ? L’article présente en premier lieu l’importance des cultures transgéniques TH dans
le monde ainsi que dans les essais au champ aux Etats-Unis. Puis à partir du cas du soja tolérant au glyphosate, il examine les
facteurs d’adoption de cette culture aux Etats-Unis où elle est très largement implantée, les avantages et les inconvénients au
niveau agro-économique. Dans un troisième temps on étudie l’emploi des herbicides sur deux cultures transgéniques par...
Tipo: Journal Article
Palavras-chave: BIOTECHNOLOGIE VEGETALE; DESHERBAGE; GLYPHOSATE GMO; TRANSGENIC CROP;
BIOTECHNOLOGY; HERBICIDE; PESTICIDE; WEEDING; HERBICIDE TOLERANCE; GLYPHOSATE; WEED;
SOYBEAN; CORN; MAIZE; ENVIRONMENT; HERBICIDE RESISTANCE; RISK.
Ano: 2008
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009e94f207d&uri=/notices/prodinra1/2009/03/
About minimal time impulsive control of sequential batch reactors with several species
Provedor de dados: 189
Autores: Rapaport, A.; Gajardo, P.; Ramirez, H.
We consider the optimal control problem of feeding in minimal time a tank where several species compete on a single resource.
We allow controls to be bounded measurable functions of time as well as impulses. For the one species case, we extend former
results given by Moreno [12] to the framework of impulse controls. For the two species case with increasing growth functions, we
show that the solution of the minimal time problem consists in either one impulse control at initial time filling the whole tank,
either reaching a singular arc. This last one is optimally reached with an impulse or waiting, and consists in keeping the system
at a constant resource level until the tank is filled. We give conditions under which the first strategy is optimal, as well...
Tipo: Conference Paper
Palavras-chave: SYSTEME DE CONTROLE; CONTROLE OPTIMAL; ETUDE DE CAS; TRAITEMENT DES DECHETS DE
L’EAU; QUALITE DE L’EAU CONTROL SYSTEM; SEQUENTIAL BATCH REACTORS; OPTIMAL CONTROL;
BIOTECHNOLOGY.
Ano: 2007
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20087a52ad38&uri=/notices/prodinra1/2008/08/
How have opinions about GMOs changed over time? The situation in the European Union and the
USA
Provedor de dados: 189
Autores: Bonny, S.
Ce texte étudie l’évolution des opinions envers les biotechnologies et les OGM depuis une dizaine d'années, en se centrant sur le
cas de l'UE et des USA. Une méta-analyse des résultats d'un grand nombre d'enquêtes et de sondages est effectuée pour comparer
les manières de voir aux Etats-Unis et dans les pays de l'Union européenne (UE) et leur évolution au fil du temps. En premier lieu
on présente les questions de méthode et les difficultés soulevées par cet exercice. Puis l’auteur examine les points de vue des
années passées aux Etats-Unis et dans l’UE qui montrent la différence de niveau d’acceptation des OGM de part et d’autre de
l’Atlantique. Ensuite il analyse l'évolution des perceptions envers les OGM d'une part aux Etats-Unis, d'autre part dans les...
Tipo: Journal Article
Palavras-chave: BIOTECHNOLOGIE; CHANGEMENT; OPINION; PERCEPTION; SONDAGE; META ANALYSE;
OPPOSITION; CONTROVERSE; PUBLIC GMO; BIOTECHNOLOGY; OPINION; PERCEPTION; SURVEY; POLL;
EUROPEAN UNION; OPPOSITION; CHANGE; META-ANALYSIS; CONTROVERSY; CONSUMER.
Ano: 2008
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008db46bb89&uri=/notices/prodinra1/2009/01/
Molecular Bio-imprinting of Biocatalysts
Provedor de dados: 17
Autores: Israr Khan; Muhammad Waheed Akhtar.
Energy conservation is the cry of the day. Attempts are made all over the world to occupy and use energy reserves. Increased
industrialization and mechanization has led to the depletion of natural energy reserves. Its unavoidable to search for renewable
sources of energy, which may be not used now but can be used by future generations. We are using the expertise of our ancestors.
Thus exploiting the nature and newer techniques in this area would yield the best results. Bio-imprinting is one of those
techniques whereby chemical modification is done in order to achieve highly expressed protein which can be stored in its highly
active form in the specific solvent.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Ecology; Genetics & Genomics;
Microbiology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Earth & Environment; Plant Biology;
Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5665/version/1
On Asymmetry in Biology and Nature
Provedor de dados: 17
Autores: I. C. Baianu.
Symmetry has attracted a substantial amount of effort because considerable simplifications are possible in the mathematical and
physical treatment of phenomena and natural systems that possess a certain degree of symmetry. Among physical and chemical
systems the most widely known are those related to crystals and fluids. Whereas crystals have a lattice structure and a symmetry
caused by ‘perfect’ order which can be classified by mathematical symmetry groups, most fluids have
an average isotropic, highly-disordered ‘structure’ that is often considered to be random.Asymmetry
is widely encountered in Biology and ecological systems- from amino acids to trees forests, and tribes, from physiological
processes to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Ecology; Genetics & Genomics; Microbiology;
Molecular Cell Biology; Bioinformatics; Earth & Environment; Plant Biology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7134/version/1
BBSRC Data Sharing Policy
Provedor de dados: 17
Autores: Amanda Collis; David McAllister; Michael Ball.
BBSRC recognizes the importance of contributing to the growing international efforts in data sharing. BBSRC is committed to
getting the best value for the funds we invest and believes that making research data more readily available will reinforce open
scientific inquiry and stimulate new investigations and analyses. BBSRC supports the view that data sharing should be led by the
scientific community and driven by scientific need. It should also be cost effective and the data shared should be of the highest
quality. Members of the community are expected and encouraged to practice and promote data sharing, determine standards and
best practice, and create a scientific culture in which data sharing is embedded.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Immunology; Microbiology; Molecular
Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Plant Biology; Evolutionary Biology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6015/version/1
The U.S. Patent System and Developing Country Access to Biotechnology: Does the Balance Need
Adjusting?
Provedor de dados: 31
Autores: Taylor,
Michael R.; Cayford,
Jerry.
Many agricultural and food security experts believe that biotechnology has potential to assist developing country farmers in
meeting current and future food needs. Most of the tools of biotechnology have been developed, however, by companies,
governments, and universities in industrialized nations; are the subject of U.S. patents; and have so far been applied commercially
to address the needs of large-scale growers in the United States and other developed countries. For commercial and other reasons,
applications of biotechnology that might benefit developing country farmers are unlikely in the foreseeable future to be developed
and disseminated through commercial channels. At the same time, noncommercial, public sector researchers report that their
access...
Tipo: Working or Discussion Paper
Palavras-chave: United States patents; Agricultural biotechnology; Developing countries; Food security; Research and
Development/Tech Change/Emerging Technologies; O34; Q16.
Ano: 2002
URL: http://purl.umn.edu/10872
ECONOMIC ISSUES IN AGRICULTURAL BIOTECHNOLOGY
Provedor de dados: 31
Autores: Shoemaker,
Hoffman,
Robbin A.; Harwood,
Linwood A.; Klotz-Ingram,
Fernandez-Cornejo,
Joy L.; Day-Rubenstein,
Cassandra; Lin,
Kelly A.; Dunahay,
William W.; Mitchell,
Terry; Heisey,
Lorraine; McBride,
Paul W.;
William D.;
Jorge.
Agricultural biotechnology has been advancing very rapidly, and while it presents many promises, it also poses as many
questions. Many dimensions to agricultural biotechnology need to be considered to adequately inform public policy. Policy is
made more difficult by the fact that agricultural biotechnology encompasses many policy issues addressed in very different ways.
We have identified several key areas — agricultural research policy, industry structure, production and marketing, consumer
issues, and future world food demand — where agricultural biotechnology is dramatically affecting the public policy agenda. This
report focuses on the economic aspects of these issues and addresses some current and timely issues as well as longer term issues.
Tipo: Report
Palavras-chave: Biotechnology; Economics; Adoption; Patents; Research policy; Markets; Market segmentation; Identity
preservation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/33735
A MODEL OF PESTICIDE RESISTANCE AS A COMMON PROPERTY AND EXHAUSTIBLE
RESOURCE
Provedor de dados: 31
Autores: Secchi,
Silvia; Babcock,
Bruce A.
A dynamic farm production model analyzes the interaction between the externalities caused by pest mobility and the development
of pesticide resistance, a nonrenewable resource, in the context of agricultural biotechnologies. The model measures the effect of
farmers' myopic behavior and the impact of pest mobility on the path of resistance.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Common property resource; European Corn Borer; Externalities; Pesticide resistance;
Intertemporal optimization; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21664
The Entrepreneur's Choice of Location: Evidence from the Life Sciences
Provedor de dados: 31
Autores: Kolympiris,
Christos; Klein,
Peter G.; Kalaitzandonakes,
Nicholas G.
Why do biotech firms cluster? New and established firms in biotech clusters are said to capitalize on knowledge spillovers,
labor-market pooling, and other externalities. Some have even argued that such spillovers are so strong that the cluster itself,
rather than the individual, is the “ locus of entrepreneurship.” Such arguments, however, do not resolve the mechanism by which
clusters might contribute to the establishment of new firms. This paper proposes a conceptual framework for analyzing the
locational choices of entrepreneurial firms in the life-sciences industry. Building on both the cluster literature and the literature on
entrepreneurship, we develop hypotheses about how cluster characteristics, the entrepreneur’ s personal characteristics, and...
Tipo: Conference Paper or Presentation
Palavras-chave: Entrepreneurship; Biotechnology; Clusters; Knowledge spillovers; Agglomeration economies; Industrial
Organization; L26; L65; O18; O32.
Ano: 2007
URL: http://purl.umn.edu/9761
FACTORS INFLUENCING ORGANIZATIONAL STRUCTURE IN THE FOOD
MANUFACTURING, CHEMICAL, AGRICULTURAL WHOLESALING AND
BIOTECHNOLOGY INDUSTRIES
Provedor de dados: 31
Autores: Roucan-Kane,
Maud; Pena,
Inaki; Boehlje,
Michael; Akridge,
Jay T.
The objective of this study is to identify factors determining a business investment strategy (i.e., the choice of investment
commitment and form of organizational structure) in the food manufacturing, chemical, agricultural wholesaling and
biotechnology industries. Propositions regarding strategic alliance theories are tested on over 400 inter-firm collaborative
agreements using secondary data from major US and European companies for the 1994-97 period. Results suggest that
transactions with lower technological and resource uncertainty levels are more likely to result in investments with a higher
commitment level (i.e., acquisitions or majority equity-based controlling investments). The investment commitment level
embedded in a single business transaction...
Tipo: Working or Discussion Paper
Palavras-chave: Transaction costs; Strategic alliances; Food manufacturing; Chemical; Agricultural wholesaling;
Ag-biotechnology; Investment strategy; Innovation; Agribusiness; Industrial Organization; L10.
Ano: 2009
URL: http://purl.umn.edu/47454
Indian Farmers' Valuation of Crop Yield Distributions: Will poor farmers value 'pro-poor' seeds?
Provedor de dados: 31
Autores: Lybbert,
Travis J.
Potential poverty traps among the rural poor suggest a need to reduce poor farmers' vulnerability by stabilizing crop yields and
limiting yield losses. Advances in agricultural biotechnology enable breeders to address this need more directly than ever before
with crops that reduce production risk by tolerating climate fluctuation or resisting biotic stresses. Will poor farmers who could
benefit most from less vulnerability choose to purchase such risk-reducing seeds? I use data from a household survey and
experiment involving farmers in India to infer their valuation of changes in the mean, variance, and skewness of yield
distributions. I conclude that these farmers value increases in expected yield in the yield distribution but seem indifferent about...
Tipo: Conference Paper or Presentation
Palavras-chave: Poverty; Risk; Biotechnology; Experimental Economics; Farm Management; C9– ; D8; O1; Q1.
Ano: 2005
URL: http://purl.umn.edu/19160
PUBLIC SECTOR PLANT BREEDING IN A PRIVATIZING WORLD
Provedor de dados: 31
Autores: Thirtle,
Colin G.; Srinivasan,
Chittur S.; Heisey,
Paul W.
Intellectual property protection, globalization, and pressure on public budgets in many industrialized countries have shifted the
balance of plant breeding activity from the public to the private sector. Several economic factors influence the relative shares of
public versus private sector plant breeding activity, with varying results over time, over country, and over crop. The private sector,
for example, dominates corn breeding throughout the industrialized world, but public and private activities in wheat breeding
differ widely in Western Europe, different regions of the United States, Canada, and Australia. Public sector involvement in plant
breeding may have benefits to society that the private sector's activities may not, fostering greater sharing of...
Tipo: Report
Palavras-chave: Plant breeding; Economics; Public sector; Private sector; Research policy; Biotechnology; Intellectual property;
Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/33775
An Analysis of Pricing in the U.S. Cotton Seed Market
Provedor de dados: 31
Autores: Shi,
Guanming; Stiegert,
Kyle W.; Chavas,
Jean-Paul.
The purpose of the research in this paper is to investigate the impact of differentiated vertical strategies by agricultural
biotechnology firms in the U.S. cottonseed market. The model advances the measurement of industry concentration to consider
substitution/complementarity relationships among differentiated products delivered under different vertical structures. We find
evidence of sub-additive pricing in the stacking of bundled biotech traits. Prices paid by farmers for cottonseed sold under vertical
integration are found to be higher than under licensing. The model is flexible and allows for evaluation of the effects of changing
market structures. The parameters on traditional measures of concentration indicate that higher concentration leads to...
Tipo: Conference Paper or Presentation
Palavras-chave: Modal Vertical Strategy; Imperfect competition; Cotton seed; Biotechnology; Agricultural and Food Policy;
Demand and Price Analysis; Industrial Organization; L13; L4; L65.
Ano: 2009
URL: http://purl.umn.edu/51617
INFORMATION POLICY AND GENETICALLY MODIFIED FOOD: WEIGHING THE
BENEFITS AND COSTS
Provedor de dados: 31
Autores: Teisl,
Mario F.; Caswell,
Julie A.
The labeling of genetically modified foods (GMFs) is the topic of a debate that could dramatically alter the structure of the U.S.
and international food industry. The current lack of harmonization of policy across countries makes GMF labeling an international
trade issue. The U.S. and Canada do not require GMFs to be labeled unless the GMF is significantly different than the
conventional food or the GMF presents a health concern. However, many other countries are requiring GMFs to be labeled. This
paper discusses empirical work on the sources and magnitude of benefits and costs from labeling programs, with particular
emphasis on the impact of the design of the labeling program on benefits and costs.
Tipo: Working or Discussion Paper
Palavras-chave: GMOs; Biotechnology; Labeling; Benefits; Costs; Food Consumption/Nutrition/Food Safety; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/14534
Is Monsanto Leaving Money on the Table? Monopoly Pricing and Bt Cotton Value with
Heterogeneous Adopters
Provedor de dados: 31
Autores: Oehmke,
James F.; Wolf,
Christopher A.
We examine the allocation of technology rents between a price-setting, innovating monopolist and heterogeneous technology
adopters. A model of monopoly pricing in the presence of heterogeneous adopters is used to examine conditions under which
greater producer (farmer) heterogeneity leads to greater producer benefit from innovation in non-competitive markets. An
application to Bt cotton determines the profit-maximizing price of Bt cotton seed and reveals that Monsanto and Delta and Pine
Land are indeed leaving money on the table in the form of unexploited profit opportunities. However, we estimate that the
presence of heterogeneous adopters explains over 80% of the rents that accrue to the farmers.
Tipo: Journal Article
Palavras-chave: Biotechnology; Bt cotton; Heterogeneous adopters; Innovation; Monopoly pricing; Technology; Valuation
distribution; L1; O3; Q1.
Ano: 2004
URL: http://purl.umn.edu/43469
THE ROLE OF SOCIOECONOMIC FACTORS AND LIFESTYLE VARIABLES IN ATTITUDE
AND THE DEMAND FOR GENETICALLY MODIFIED FOODS
Provedor de dados: 31
Autores: Heiman,
Amir; Just,
David R.; Zilberman,
David.
Consumer resistance is a key barrier to the diffusion of genetically modified foods (GMFs). Several studies have shown that
consumers in general have a negative attitude toward GMFs. Through analysis of a survey conducted in Israel, we find consumer
attitudes toward GMFs to be context specific, differing based on the available alternatives. Consumers responded positively to
genetically modified meats when given the alternative choice of meats produced with hormones or dyes. We also address the
importance of gender, education, and being religious on the consumer's attitude toward GMFs. Both education and being religious
have significant effects on attitude, while gender does not.
Tipo: Journal Article
Palavras-chave: Biotechnology; Consumer attitudes; Diffusion; Genetically modified foods; Food Consumption/Nutrition/Food
Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2000
URL: http://purl.umn.edu/14713
TO REACH THE POOR: RESULTS FROM THE ISNAR-IFPRI NEXT HARVEST STUDY ON
GENETICALLY MODIFIED CROPS, PUBLIC RESEARCH, AND POLICY IMPLICATIONS
Provedor de dados: 31
Autores: Atanassov,
Ebora,
Atannas; Bahieldin,
Reynaldo V.; Falck-Zepeda,
Emeka; Odhiambo,
Ana; Smale,
Ahmed; Brink,
Johan; Burachik,
Jose Benjamin; Herrera-Estrella,
Benjamin; Quemada,
Melinda; Sutrisno; Valyasevi,
Hector; Peng,
Ruud; Zafar,
Moises; Cohen,
Luis; Komen,
Yufa; Sampaio,
Joel I.; Dhawan,
John; Low,
Fee Chon; Omaliko,
Maria Jose; Sithole-Niang,
Yusuf; Zambrano,
Vibha;
Idah; Sittenfeld,
Patricia.
Local farming communities throughout the world face productivity constraints, environmental concerns, and diverse nutritional
needs. Developing countries address these challenges in a number of ways. One way is public research that produces genetically
modified (GM) crops and recognize biotechnology as a part of the solution. To reach these communities, GM crops, after
receiving biosafety agreement, must be approved for evaluation under local conditions. However, gaps between approvals in the
developed and developing world grow larger, as the process of advancing GM crops in developing countries becomes increasingly
difficult. In several countries, only insect resistant cotton has successfully moved from small, confined experimental trials to
larger, open...
Tipo: Working or Discussion Paper
Palavras-chave: Biosafety; Regulation; Biotechnology; Genetic modification; Public research; Crop Production/Industries;
Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/16065
Major Forces and Factors Shaping Global Markets: The Emerging Agricultural World
Provedor de dados: 31
Autores: Brookins,
Carole L.
The world is producing greater opportunity for more people than ever before in the history of mankind. The global agri-food
system is dynamic, not static. In order to understand the future trends and potentials for utilizing and improving our agri-food
system, it is critical to focus on forces that will improve both productivity and policy to meet the demands of the ever-changing
global market. This paper identifies five major trends that will impact the global economy and create structural change in
commodity markets.
Tipo: Journal Article
Palavras-chave: Biotechnology; Energy; Environment; Farm policy; Food safety; Information technology; Infrastructure gaps;
Trade logistics; Agribusiness; Food Consumption/Nutrition/Food Safety.
Ano: 2007
URL: http://purl.umn.edu/62280
The Biotechnology Sector: "Bounds" to Market Structure
Provedor de dados: 31
Autores: Sheldon,
Ian M.
This paper examines whether it makes sense to consider Sutton's "bounds" approach as a candidate theory for explaining the
recent evolution of market structure in the biotechnology sector, and to speculate whether market structure will change if the
industry begins to introduce second-generation GM products that are of more direct benefit to consumers. A key result is that the
market structure is bounded in the presence of endogenous sunk costs, implying care should be taken when inferring any
correlation between R&D expenditure and seller concentration in the biotechnology sector.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Market structure; Research and Development/Tech Change/Emerging Technologies; L1; L11.
Ano: 2008
URL: http://purl.umn.edu/6078
Regional Biotechnology Regulations: Design Options and Implications for Good Governance
Provedor de dados: 31
Autores: Birner,
Regina; Linacre,
Nicholas A.
Many developing countries are currently in the process of designing regulatory systems that should allow them to use genetically
modified organisms (GMOs) for agricultural development, while also managing the food safety and environmental risks
potentially associated with these technologies. Various regions of the developing world are seeking to establish regional systems
of biotechnology regulation. However, considerable costs are associated with biotechnology regulation, and biosafety specialists
are scarce. In addition, there is no consistent understanding of how regional systems of biotechnology regulation can be designed
to be effective and efficient, while also fulfilling the principles of good governance, such as transparency, voice and...
Tipo: Working or Discussion Paper
Palavras-chave: Regional biotechnology regulation; Regulatory federalism; Transaction cost economics; West Africa; European
Union; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/42346
Estimating the Adoption of Bt Eggplant in India: Who Benefits from Public-Private Partnership?
Provedor de dados: 31
Autores: Krishna,
Vijesh V.; Qaim,
Matin.
The study analyzes ex-ante the adoption of insect resistant Bt eggplant in India. Farmers' willingness to pay (WTP) is estimated
using the contingent valuation method. Given the economic importance of insect pests, the average WTP for proprietary Bt
hybrids is more than four times the current price of conventional hybrids. Since the private innovating firm has shared its
technology with the public sector, it is likely that public open-pollinated Bt varieties will also be released after a small delay. This
will reduce farmers' WTP for Bt hybrids by 35%, thus decreasing the scope for corporate pricing policies. Nonetheless, ample
profit potential remains. Analysis of factors influencing farmers' adoption decisions demonstrates that public Bt varieties will...
Tipo: Conference Paper or Presentation
Palavras-chave: Public-private partnership; Biotechnology; Bt eggplant; Adoption; Willingness to pay; India; Crop
Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/25311
Consumers' Willingness to Pay for Genetically Modified Foods in Kenya
Provedor de dados: 31
Autores: Kimenju,
Simon Chege; De Groote,
Hugo.
A survey of 600 consumers was conducted in Nairobi to determine attitudes and willingness to pay (WTP) for GM maize meal.
WTP was estimated using the double-bounded logit model. Overall, 38% are aware of GM crops. Most consumers believe in the
technology's positive impacts, but are concerned about environmental and health risks. Majority (68%) would buy GM maize
meal at the price of their favourite maize meal brand. The mean WTP for GM maize meal is KShs 58 for a 2kg packet, a premium
of 13.7 % over mean average price of favourite brands. WTP is influenced more by subjective than socioeconomic characteristics.
Tipo: Conference Paper or Presentation
Palavras-chave: GMO; Biotechnology; Consumer; Africa; Maize; Consumer/Household Economics; D12; C25; Q16.
Ano: 2005
URL: http://purl.umn.edu/24504
THE REGULATORY REGIME AND ITS IMPACT ON INNOVATION ACTIVITIES IN
AGRO-FOOD BIOTECHNOLOGY IN THE EU AND USA
Provedor de dados: 31
Autores: Menrad,
Klaus.
Paper prepared for presentation at the Conference: “Seeds of Change: Intellectual Property Protection for Agricultural
Biotechnology” University of Illinois at Urbana-Champaign (USA), April 8 to 10th, 2004
Tipo: Conference Paper or Presentation
Palavras-chave: Agro-food Biotechnology; Innovation; European Union; United States; Regulations; Industrial Organization;
L5; L66; O32.
Ano: 2004
URL: http://purl.umn.edu/91014
PUBLIC OPINION OF AGBIOTECH IN THE US AND UK: A CONTENT ANALYSIS
APPROACH
Provedor de dados: 31
Autores: Kalaitzandonakes,
Nicholas G.; Marks,
Leonie A.
In this paper we use content analysis to show trend in public opinion of agrobiotechnology in the United Kingdom (UK) and the
United States (US). We test for the degree of positive and negative content in mass media reporting over the period 1995-1998.
Specifically, we test whether there are qualitative and quantitative differences between three national daily newspapers - the Daily
Telegraph (UK), USA Today (US), and the Washington Post (US) - based on reporting of agrobiotechnology. Results indicate
that content has become more negative in the UK over the time period while reporting the US has not significantly changed.
Results indicate that both the BSE and Pusztai crises had an impact on the proportion of negative reporting in the Daily Telegraph.
Tipo: Conference Paper or Presentation
Palavras-chave: Content analysis; Mass media reporting; Agricultural biotechnology; Research and Development/Tech
Change/Emerging Technologies; Teaching/Communication/Extension/Profession.
Ano: 1999
URL: http://purl.umn.edu/21678
WHEAT CHARACTERISTIC DEMAND AND IMPLICATIONS FOR DEVELOPMENT OF
GENETICALLY MODIFIED GRAINS
Provedor de dados: 31
Autores: Janzen,
Edward L.; Mattson,
Jeremy W.; Wilson,
William W.
Agricultural biotechnology is advancing rapidly and is embracing all major crops. The adoption of genetically modified corn,
soybeans, and cotton have reached high levels in the United States. Wheat is the next major crop confronting the biotechnology
issue, but no commercial varieties of genetically modified (GM) wheat have been released yet. Primary opportunities for GM
developments in wheat center around improvements that meet consumer and end-user needs/issues in addition to meeting
producer efficiencies. Developers and proponents of GM wheat must focus on education of consumers and restoration of
worldwide confidence in the regulatory systems. The most desired wheat quality trait among millers and bakers is the assurance
of consistent quality.
Tipo: Working or Discussion Paper
Palavras-chave: Wheat; Wheat-based foods; Agricultural biotechnology; Genetically modified; GM; Herbicide-tolerant;
Functional foods; Nutraceuticals.; Crop Production/Industries.
Ano: 2001
URL: http://purl.umn.edu/23508
VERTICAL AND HORIZONTAL COORDINATION IN THE AGRO-BIOTECHNOLOGY
INDUSTRY: EVIDENCE AND IMPLICATIONS
Provedor de dados: 31
Autores: Kalaitzandonakes,
Nicholas G.; Bjornson,
Bruce.
Agro-biotechnology is evolving from a pre-commercial phase dominated by basic research science to a commercial phase oriented
around marketing products. In pursuing innovation rents in the commercial phase, firms are reorienting their strategies around
complementary marketing and distribution assets. This is impacting vertical and horizontal industry structure. Conversely,
industry structure is also impacting firm strategies. Horizontal alliances and consolidation continue from the pre-commercial
phase into the commercial phase, while vertical coordination and integration strategies are accelerating rapidly.
Interplay
between firm strategy and industry structure is too complex for firms to anticipate early in the pre-commercial phase for
long-term...
Tipo: Journal Article
Palavras-chave: Acquisitions; Agricultural biotechnology; Firm strategy; Industry consolidation; Industry structure; Mergers;
Industrial Organization; Research and Development/Tech Change/Emerging Technologies.
Ano: 1997
URL: http://purl.umn.edu/15537
Ecological Risks of Novel Environmental Crop Technologies Using Phytoremediation as an Example
Provedor de dados: 31
Autores: Angle,
J. Scott; Linacre,
Nicholas A.
Phytoremediation is the use of living plants, known as hyperaccumulators which absorb unusually large amounts of metals in
comparison to other plants. The use of classical plant breeding and new molecular techniques offers great potential to develop
crops with the ability to clean up polluted sites. While these technologies have gained widespread attention, prior to commercial
development, there are risks that must be considered – only a few of which have received even modest examination. Therefore,
the focus of this working paper is to explore specific risks associated with phytoremediation and suggest ways in which these risks
can be managed so that new, novel, and innovative plant technologies may be applied to provide low cost and efficient...
Tipo: Working or Discussion Paper
Palavras-chave: Risk; GMO; Biotechnology; Phytoremediation; Phytoextraction; Phytomining; Environmental risk; Crop
Production/Industries; Q16; Q2.
Ano: 2005
URL: http://purl.umn.edu/59225
On Pricing and Vertical Organization of Differentiated Products: The Case of Soybean Seed Industry
Provedor de dados: 31
Autores: Shi,
Guanming; Chavas,
Jean-Paul.
This paper investigates the pricing and vertical organization of differentiated products under imperfect competition. In a
multiproduct context, a Cournot model is used to examine how substitution/complementarity relationships among products and
vertical structures can affect the exercise of market power. This motivates a generalization of the Herfindahl-Hirschman index
(termed VHHI) capturing how market concentration and vertical structures interact to influence prices of differentiated products.
The analysis is applied to pricing of soybean seeds in the US over the period 2000-2007. The analysis considers two vertical
structures employed by biotech firms: vertical integration and licensing. The econometric analysis finds evidence that vertical...
Tipo: Conference Paper or Presentation
Palavras-chave: Vertical structures; Pricing; Imperfect competition; Seed; Biotechnology; Demand and Price Analysis; Industrial
Organization; L13; L4; L65.
Ano: 2009
URL: http://purl.umn.edu/49186
PUBLIC-PRIVATE AGREEMENTS FOR EMERGING TECHNOLOGIES IN AGRICULTURE
Provedor de dados: 31
Autores: Johnson,
Marc A.
Land Grant Universities are partnering with private firms to fulfill the university mission. Public and private entities can come
together in mutually beneficial activities, measured by fulfillment of each party's mission. Rules of engagement are discussed. Six
actual agreements currently in operation between Kansas State University and private entities represent a range of types of
public-private agreements. The principal components of each agreement are described and the benefits to each party enumerated
in the context of organizational missions.
Tipo: Journal Article
Palavras-chave: Biotechnology; Commercialization; Intellectual property; Joint venture; Land Grant Universities; Nonprofit
corporation; Patent licensing; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/14687
Biotecnologia: Perfil dos Grupos de Pesquisa no Brasil
Provedor de dados: 31
Autores: Mendonca,
Marco Aurelio Alves de; Freitas,
Rogerio Edivaldo.
O artigo objetiva mapear o perfil do capital humano envolvido com a biotecnologia no Brasil. Empregou-se análise estatística
descritiva. A base de dados foi exclusivamente montada para esta pesquisa a partir de informações do Portal de Inovação, do
Ministério da Ciência e Tecnologia. A investigação constatou a existência de importante incremento dos grupos de pesquisa
relacionados ao tema, nos últimos anos. Tal fato resulta dos esforços empreendidos por uma série de agentes, sendo o governo
federal o principal. Ademais, os dados indicam que os pesquisadores concentram-se especialmente nas regiões Sul e Sudeste, com
significativa participação das ciências Agrárias, Biológicas e da Saúde.--------------------The article aims to map the human capital
profile...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotecnologia; Capital Humano; Análise Descritiva; Biotechnology; Human Capital; Descriptive Analysis;
Labor and Human Capital; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/108141
THE IMPACT OF REGULATION ON THE DEVELOPMENT OF NEW PRODUCTS IN THE
FOOD INDUSTRY
Provedor de dados: 31
Autores: Menrad,
Klaus; Blind,
Knut.
Paper prepared for presentation at the 8th ICABR International Conference on Agricultural Biotechnology: International Trade
and Domestic Production Ravello (Italy), July 8 to 11th, 2004
Tipo: Conference Paper or Presentation
Palavras-chave: Food industry; Regulation; Innovation; Biotechnology; Organic food; Functional Food; European Union; United
States; Industrial Organization; L5; L66; O32.
Ano: 2004
URL: http://purl.umn.edu/91013
Safety and Socio-Economic Issues Raised by Modern Biotechnology
Provedor de dados: 31
Autores: Xue,
Dayuan; Tisdell,
Clement A.
Modern new biotechnology has the potential to provide major economic and other benefits, but at the same time it poses potential
hazards for human health, the environment, the ‘natural’ biological order and can have adverse socio-economic consequences.
The application of such technology frequently violates traditional ethical, moral and religious values. This paper after outlining
possible benefits of modern new biotechnologies, discusses the type of biosafety risks which they pose, their possible adverse
consequences for the sustainability of biodiversity and agriculture and their potential impacts on socioeconomic welfare and
traditional cultures. Particular concern is expressed about the possible consequences of such technologies for developing...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Socio-economic policy; Genetic engineering; Biodiversity; Biosafety; Patents; Environmental
Economics and Policy; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/47995
BIOTECHNOLOGY IN AGRICULTURE: IMPLICATIONS FOR FARM-LEVEL RISK
MANAGEMENT
Provedor de dados: 31
Autores: Makki,
Shiva S.; Somwaru,
Agapi; Harwood,
Joy L.
This study examines the risks associated with adoption of biotech crops and discusses their implications for risk management at
the farm level. We develop an analytical risk evaluation matrix framework to illustrate changes in production and marketing risks
of biotech and non-biotech crops. Price uncertainty generated by consumer concerns is the major risk facing biotech farmers,
while cross-pollination with biotech crops and preservation of non-biotech status are major concerns for non-biotech farmers.
Improved market infrastructure to handle biotech products and modification of the current risk management tools to accommodate
new risks are essential in reducing the farm-level risks.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Marketing risks; Production risks; Risk evaluation matrix; Risk
management; Research and Development/Tech Change/Emerging Technologies; Risk and Uncertainty.
Ano: 2001
URL: http://purl.umn.edu/14683
Estimate of the Potential Adoption of Genetically Modified Cotton by Farmers in Southern Spain and
its Economic Implications
Provedor de dados: 31
Autores: Gomez-Barbero,
Manuel; Rodgriguez-Cerezo,
Emilio.
This paper analyses the potential adoption and economic on-farm impact of Bt cotton in the Andalusia region (southern Spain).
Survey data have shown that, on average, 58% of the responding farmers know about genetically modified (GM) cotton. Another
outcome of the survey is that 95% of the farmers declaring that they know about GM cotton would be willing to grow Bt cotton.
In the main cotton-producing districts, farmers' attitudes are positive, and they would be willing to adopt the technology. In areas
where the crop is more important, the farmers who are willing to adopt Bt cotton represent over 75% of the area under cotton. As
regards the on-farm economic impact resulting from a potential adoption, the assessment is that savings on the cost of direct...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Cotton; Economic impact; Andalusia; Agricultural and Food Policy; Q16; Q20; Q12; O52.
Ano: 2005
URL: http://purl.umn.edu/24556
Can Technology Deliver on the Yield Challenge to 2050?
Provedor de dados: 31
Autores: Fischer,
R.A.; Byerlee,
Derek R.; Edmeades,
Gregory O.
This paper was produced for the FAO Expert Meeting on How to Feed the World in 2050 (Rome, 24-26 June 2009).
Tipo: Conference Paper or Presentation
Palavras-chave: Technology; Productivity; Yield gaps; Investment in R&D; Biotechnology; Input efficiency; Research and
Development/Tech Change/Emerging Technologies.
Ano: 2009
URL: http://purl.umn.edu/55481
Pecuniary, Non-Pecuniary, and Downstream Research Spillovers: The Case of Canola
Provedor de dados: 31
Autores: Gray,
Richard S.; Malla,
Stavroula; Tran,
Kien C.
This paper develops an empirical framework for estimating a number of inter-firm and downstream research spillovers in the
canola crop research industry. The spillovers include basic research, human capital/ knowledge (as measured through other-firm
expenditures), and genetics (as measured through yields of other-firms). The model used to examine spillover effects on research
productivity provides evidence that there are many positive inter-firm non-pecuniary research spillovers, which is consistent with
a research clustering effect. The second model, which examines spillovers at the level of firm revenue , shows that, while private
firms tend to crowd one another, public firm expenditure on basic and applied research creates a crowding-in effect for...
Tipo: Conference Paper or Presentation
Palavras-chave: Basic research; Applied research; Public research expenditures; Private research expenditures; Biotechnology;
Research and Development/Tech Change/Emerging Technologies; O3.
Ano: 2005
URL: http://purl.umn.edu/24776
Public Attitudes towards Agricultural Biotechnology
Provedor de dados: 31
Autores: Hoban,
Thomas J.
The full benefits of agricultural biotechnology will only be realized if consumers and food manufacturers consider it safe and
beneficial. Although few internationally comparable public opinion surveys have been conducted on this issue, the available
evidence suggests that public attitudes differ sharply both between and within countries and are evolving over time. Consumer
attitudes have been studied in the United States and Europe for more than a decade. This research is reviewed along with the
available data from Latin America, Asia and Africa. Results from the largest internationally comparable public opinion survey on
agricultural biotechnology to date are highlighted.
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural Biotechnology; Public Opinion; Consumer Acceptance.; Research and Development/Tech
Change/Emerging Technologies; D12; 013; Q18.
Ano: 2004
URL: http://purl.umn.edu/23810
The Private Sector in Agricultural R&D: Policies and Institutions to Foster its Growth in
Developing Countries
Provedor de dados: 31
Autores: Naseem,
Anwar; Omamo,
Steven Were; Spielman,
David J.
New technologies are critical to enhancing agricultural productivity and reducing poverty in many developing countries. While
public-sector investment in research has historically driven technological change in agriculture, recent trends suggest that the
public sector’s role may not be as significant in the future. There is much optimism about the private sector’s capacity to deliver
new technologies, even though current levels of private investment in research in developing countries remain low. This paper
examines the determinants of private investment in agricultural research and development in developing countries, the market and
institutional constraints that limit private investment growth, and the incentive mechanisms that can strengthen private...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural research and development; Private sector; Agricultural productivity; Research and development;
Poverty reduction; Investments; Biotechnology; Food policy; Agricultural research; Research and Development/Tech
Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/58582
Potential Impacts of Bt Eggplant on Economic Surplus and Farmers’ Health in India
Provedor de dados: 31
Autores: Krishna,
Vijesh V.; Qaim,
Matin.
In this article, the potential impacts of Bt eggplant technology in Indian agriculture are analyzed. Several proprietary Bt hybrids
are likely to be commercialized in the near future. Based on field trial data, it is shown that the technology can significantly reduce
insecticide applications and increase effective yields. Comprehensive farm survey data are used to project farm level effects and
future adoption rates. Simulations show that the aggregate economic surplus gains of Bt hybrids could be around US $108 million
per year. Consumers will capture a large share of these gains, but farmers and the innovating company will benefit too. As the
company has also shared its technology with the public sector, Bt open-pollinated varieties might become...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Bt eggplant; Economic surplus; Health costs; Pesticides; Public-private partnership; Health
Economics and Policy; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9909
Uncertainty aversion in Australian regulation of agricultural gene technology
Provedor de dados: 31
Autores: Gray,
Emily M.; Ahmadi-Esfahani,
Fredoun Z.
There is potential for over-provision of environmental harms and under-provision of environmental benefits associated with GM
crops. As a result, strong public regulation is needed to ensure that full social values are considered. However, one reason for
opposition to GM crops is a lack of public trust in regulatory institutions and science, and the limited opportunities afforded to
public-participation and nonscientific concerns. We aim to demonstrate the trade-off between social cost and managing the risks
of gene flow arising from environmental release of GM canola in Australia, using the framework of a probabilistic risk assessment
and safety-rule decision mechanism.
Tipo: Conference Paper or Presentation
Palavras-chave: Safety-rule; Uncertainty; Biotechnology regulation; Canola; Crop Production/Industries; Institutional and
Behavioral Economics; Research and Development/Tech Change/Emerging Technologies; Risk and Uncertainty.
Ano: 2008
URL: http://purl.umn.edu/6045
GM-free private standards and their effects on biosafety decision-making in developing countries
Provedor de dados: 31
Autores: Gruere,
Guillaume P.; Sengupta,
Debdatta.
We provide a comprehensive review of international cases where GM-free private standards set up by food companies in
developed countries have influenced biosafety policymaking in developing countries. We find twenty-nine cases where private
importers have directly or indirectly affected policy decisions in twenty-one countries. Most of the cases relate irrational fear of
export losses to excessively precautionary decisions. These cases are based on two generally misleading premises: the belief that
Europe or Japan represents the only market for exports, and the perception that non-GM segregation is infeasible or prohibitively
costly in all situations. Our study also demonstrates the importance of information asymmetries across countries and agents and
the...
Tipo: Conference Paper or Presentation
Palavras-chave: Agricultural biotechnology; Private standards; Political economics.; Agricultural and Food Policy; International
Relations/Trade; Political Economy; Q17; Q18.
Ano: 2009
URL: http://purl.umn.edu/51334
Farmer Acceptance of Genetically Modified seeds in Germany: Results of a Cluster Analysis
Provedor de dados: 31
Autores: Gyau,
Amos; Voss,
Julian; Spiller,
Achim; Enneking,
Ulrich.
www.ifama.org
Tipo: Journal Article
Palavras-chave: Biotechnology; Cluster analysis; German farmers; Agricultural and Food Policy; Crop Production/Industries;
Farm Management; Food Security and Poverty; Q2.
Ano: 2009
URL: http://purl.umn.edu/92552
Crop Research Incentives in a Privatized Industry: A Stochastic Approach
Provedor de dados: 31
Autores: Malla,
Stavroula; Gray,
Richard S.
We model today's privatized crop research industry as a small number of firms, developing and selling differentiated products to
heterogeneous producers. Crop variety research is modeled as a search process, which allows us to differentiate between applied
and basic research and recognize research as a stochastic process. We use the framework to develop a number of propositions
regarding private research incentives, the spillovers of knowledge, and the impact of public policy. The results suggest an
underinvestment in research even when property rights have been established.
Tipo: Conference Paper or Presentation
Palavras-chave: Search process; Stochastic process; Biotechnology; IPRs; Applied R&D; Basic R&D; Imperfect
competition; Differentiated products; Heterogeneous producers.; Agribusiness.
Ano: 2002
URL: http://purl.umn.edu/24936
Seeking a Common Path: Structuring Multistakeholder Dialogue on Agricultural Biotechnology in
Africa
Provedor de dados: 31
Autores: von Grebmer,
Klaus; Omamo,
Steven Were.
This paper analyzes an ongoing effort by national, regional, and international partners to raise awareness, promote dialogue, and
catalyze consensus-building mechanisms among stakeholder groups on the role of biotechnology in agricultural development and
food security in southern Africa. Stakeholders include public bodies, the private sector, and civil society. The paper argues that
while responsible adoption of agricultural biotechnology promises significant gains to the countries of southern Africa,
governments must clarify its specific role, improve policies for its application, and assess the place of biotechnology in broader
development strategies. The issues that surround the introduction, creation, and application of agricultural biotechnology in...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Consensus-building; Conflict resolution; Africa; Food security; Genetically modified food;
Biosafety; Capacity building; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/58581
Plant Genetic Resources for Agriculture, Plant Breeding, and Biotechnology: Experiences from
Cameroon, Kenya, the Philippines, and Venezuela
Provedor de dados: 31
Autores: Falck-Zepeda,
Hautea,
Jose Benjamin; Zambrano,
Desiree; Kengue,
Joseph; Songa,
Patricia; Cohen,
Joel I.; Borges,
Orangel; Guimaraes,
Elcio P.;
Josephine.
Local farming communities throughout the world face binding productivity constraints, diverse nutritional needs, environmental
concerns, and significant economic and financial pressures. Developing countries address these challenges in different ways,
including public and private sector investments in plant breeding and other modern tools for genetic crop improvement. In order to
measure the impact of any technology and prioritize investments, we must assess the relevant resources, human capacity, clusters,
networks and linkages, as well as the institutions performing technological research and development, and the rate of farmer
adoption. However, such measures have not been recently assessed, in part due to the lack of complete standardized information...
Tipo: Working or Discussion Paper
Palavras-chave: Plant breeding; Biotechnology; Public research; Funding; Innovation systems; Capacity building; Crop
Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/42329
NORTH DAKOTA SHOPPER PERCEPTIONS OF GENETICALLY MODIFIED ORGANISMS
AND FOOD: RESULTS OF A WINTER 2003 SURVEY
Provedor de dados: 31
Autores: Wachenheim,
Cheryl J.; Lesch,
William C.
Replaced with revised version of paper 07/23/04.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetic Modification; Consumer Preferences; Food Consumption/Nutrition/Food Safety;
Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/23601
Biotech--Who Wins? Economic Benefits and Costs of Biotechnology Innovations in Agriculture
Provedor de dados: 31
Autores: Moschini,
GianCarlo.
The conceptual model necessary for an assessment of biotechnology's economic benefits and costs is outlined, emphasizing the
need to account for the proprietary nature of biotechnology innovations. The model is illustrated with an application to Roundup
Ready soybeans. The estimated value of this innovation is sizeable, with consumers and innovators claiming the largest shares of
net benefits. Also, disparities in intellectual property rights protection across countries affect the distribution of benefits.
Consumer resistance toward GMOs and the issue of labelling and market segregation complicate the economic evaluation of
biotechnology innovations; a number of related regulation and public policy issues are discussed. Emerging output-trait GMOs
are...
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Identity preservation; Intellectual property rights; R&D;
Transgenic crops; Welfare evaluation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/23862
The Economic Impact of Genetically Engineered Crops
Provedor de dados: 31
Autores: Zilberman,
David; Sexton,
Steven E.; Marra,
Michele C.; Fernandez-Cornejo,
Jorge.
Outstanding Choices Article Award, 2011
Tipo: Journal Article
Palavras-chave: Biotechnology; GMO; Adoption; Innovation; Productivity; Crop Production/Industries; Q160; Q240; Q420.
Ano: 2010
URL: http://purl.umn.edu/94769
Biological Limits on Agricultural Intensification: An Example from Resistance Management
Provedor de dados: 31
Autores: Laxminarayan,
Ramanan; Simpson,
R. David.
When the application of pesticides places selective evolutionary pressure on pest populations, it can be useful to plant refuge
areas-crop areas intended to encourage the breeding of pests that are susceptible to the pesticide. Renewed interest in refuge areas
has arisen with recent advances in biotechnology and genetically modified (GM) crops. In this paper, we use a simple model of
the evolution of pest resistance to characterize the socially optimal refuge strategy for managing pest resistance. We demonstrate
some interesting analogies with other models of renewable resource management, such as those of fisheries. Among the analogous
results are findings that maintaining what we might call "maximal sustainable susceptibility" is typically not...
Tipo: Working or Discussion Paper
Palavras-chave: Pest resistance; Biotechnology; Optimization; Research and Development/Tech Change/Emerging
Technologies; Q1; Q2.
Ano: 2000
URL: http://purl.umn.edu/10914
THE SEED INDUSTRY IN U.S. AGRICULTURE: AN EXPLORATION OF DATA AND
INFORMATION ON CROP SEED MARKETS, REGULATION, INDUSTRY STRUCTURE, AND
RESEARCH AND DEVELOPMENT
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge.
The unprecedented growth in crop yields and agricultural total factor productivity over the past 70 years owes much to a series of
biological innovations embodied in seeds, beginning with the development of hybrid crops in the United States in the early part of
the 20th century, continuing with the adoption of high-yielding varieties during the Green Revolution of the 1960s and 1970s, and
more recently, modern biotechnology. Throughout this period, the seed industry evolved, as small businesses gave way to larger
enterprises that integrated plant breeding, production, conditioning, and marketing functions. The industry was further shaped by
widespread mergers and acquisitions in the latter part of the century, rapid growth - in private research and...
Tipo: Report
Palavras-chave: Seed markets; Seed costs and prices; Regulation; Plant breeding; Field crops; Research and development;
Industry concentration; Biotechnology; Agribusiness; Crop Production/Industries.
Ano: 2004
URL: http://purl.umn.edu/33671
CHINA'S FOOD AND AGRICULTURE: ISSUES FOR THE 21ST CENTURY
Provedor de dados: 31
China's impact on world agricultural markets in coming decades will depend on many factors. Growing income and transition to
modern urban lifestyles will increase demand for all foods, but demand will shift toward meat and high-value products. WTO
accession may increase openness of China's agricultural trade and allow more imports. An understanding of geographic variations
of consumption, production, trade, and policy is critical to understanding the vast China market. Development of transportation
infrastructure and market channels will make it easier for food products to reach consumers. China's approach to biotechnology
and its reform of institutions for allocating land, labor, and water inputs have important implications for agricultural productive...
Tipo: Report
Palavras-chave: China; Food; Agriculture; Production; Consumption; Regions; International trade; Biotechnology; Livestock;
Land; Irrigation; Retail; Transportation; Marketing; Rural development; Labor markets; Statistics; Agricultural and Food Policy.
Ano: 2002
URL: http://purl.umn.edu/33723
Adoption of Transgenic Crops by Smallholder Farmers in Entre Rios, Argentina
Provedor de dados: 31
Autores: Paredes,
Cecilia; Martin,
Marshall A.
This is a study of the adoption of transgenic crops by 120 smallholder farmers interviewed in July 2005 in the communities of San
Jose de Feliciano and La Paz in the Entre Rios Province of Argentina. Logistic regression results indicate that access to a planter
is essential for smallholders to adopt Bt corn, while adopters of Roundup Ready™ soybeans have larger farms, access to credit,
availability of all essential machinery, more years of schooling, and are primarily located in the La Paz community.
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic crops; Technology adoption; Bt corn; Roundup Ready™ soybeans; Biotechnology; Argentina;
Smallholders; Farm Management; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9996
Economic Benefits and Costs of Biotechnology Innovations in Agriculture
Provedor de dados: 31
Autores: Moschini,
GianCarlo.
The conceptual model necessary for an assessment of biotechnology's economic benefits and costs is outlined, emphasizing the
need to account for the proprietary nature of biotechnology innovations. The model is illustrated with an application to Roundup
Ready soybeans. The estimated value of this innovation is sizeable, with consumers and innovators claiming the larger share of
net benefits. Also, disparities in intellectual property rights protection across countries affect the distribution of benefits.
Consumer resistance toward genetically modified organisms (GMOs) and the issues of labeling and market segregation
complicate the economic evaluation of biotechnology innovations, and a number of related regulation and public policy issues are
discussed....
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetically modified organisms; Identity preservation; Intellectual property rights; R&D;
Transgenic crops; Welfare evaluation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/18413
Technology Adoption and Off-Farm Household Income: The Case of Herbicide-Tolerant Soybeans
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge; Hendricks,
Chad; Mishra,
Ashok K.
We model the interaction of off-farm work and adoption of agricultural technologies and the impact of adopting these
technologies on farm household income from on farm and off-farm sources after controlling for such interaction, and estimate the
model for the case of adoption of herbicide-tolerant (HT) soybeans using a nationwide survey of soybean farms for 2000. We
find that adoption of HT soybeans is positively and significantly related to off-farm household income for U.S. soybean farmers,
after controlling for other factors. In addition, while on-farm household income is not significantly related to adoption, total
household income increases significantly with adoption.
Tipo: Journal Article
Palavras-chave: Agricultural household model; Biotechnology; Herbicide tolerant soybeans; Off-farm income; Technology
adoption; O33; Q12.
Ano: 2005
URL: http://purl.umn.edu/43487
Analysis for biotechnology innovations using Strategic Environmental Assessment (SEA)
Provedor de dados: 31
Autores: Lanacre,
Hector; Halsey,
Nicholas A.; Gaskell,
Mark; Birner,
Joanne; Rosegrant,
Mark W.; Falck-Zepeda,
Jose Benjamin; Quemada,
Regina.
Meeting the food needs of the world’s growing population while reducing poverty and protecting the environment is a major
global challenge. Genetically modified crops appear to provide a promising option to deal with this challenge. However there is a
need to make strategic decisions on how to spend limited agricultural research funds in order to achieve a maximum impact with
regard to finding sustainable solutions to end hunger and poverty. In international development institutions, there is growing
interest in the potential use of Strategic Environmental Assessment (SEA) as part of a research based Environmental Management
System (EMS) to promote mainstreaming of environmental considerations in policy development. SEA was developed as an
approach to...
Tipo: Working or Discussion Paper
Palavras-chave: Risk; Strategic Environmental Assessment; Genetically Modified; GMO; LMO; Living Modified Organisms;
Biotechnology; Environmental risk; Environmental Economics and Policy; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2005
URL: http://purl.umn.edu/58584
Incorporating Project Uncertainty in Novel Environment Biotechnologies: Illustrated Using
Phytoremediation
Provedor de dados: 31
Autores: Linacre,
Nicholas A.; Whiting,
Steven N.; Angle,
J. Scott.
Pollution of the environment by metals and organic contaminants is an intractable global problem, with cleanup costs running into
billions of dollars using current engineering technologies. The availability of alternative, cheap and effective technologies would
significantly improve the prospects of cleaning-up metal contaminated sites. Phytoremediation has been proposed as an
economical and ‘green’ method of exploiting plants to extract or degrade the contaminants in the soil. To date, the majority of
phytoremediation efforts have been directed at leaping the biological, biochemical and agronomic hurdles to deliver a working
technology, with scant attention to the economic outlook other than simple estimates of the cost advantages of phytoremediation
over...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Project risk; Soil contaminants; Environmental remediation; Industrial crop technologies; Crop
Production/Industries; Q5; Q51; Q2.
Ano: 2005
URL: http://purl.umn.edu/59223
THE EFFECTS OF INFORMATION ON CONSUMER DEMAND FOR BIOTECH FOODS:
EVIDENCE FROM EXPERIMENTAL AUCTIONS
Provedor de dados: 31
Autores: Tegene,
Abebayehu; Huffman,
Wallace E.; Rousu,
Matthew C.; Shogren,
Jason F.
Consumers' willingness to pay for food products decreases when the food label indicates that a food product is produced with the
aid of modern biotechnology. This bulletin presents empirical evidence on consumers' willingness to pay for biotech foods based
on the presence or absence of labels advising that the food was prepared with the aid of biotechnology. The authors designed and
conducted an experimental auction to elicit consumers' willingness to pay for "genetically modified" (GM)-labeled and
standard-labeled foods under different information regimes. The evidence gathered for vegetable oil, tortilla chips, and potatoes
shows that labels matter. In particular, under all information treatments, consumers discounted food items labeled "GM" by an...
Tipo: Report
Palavras-chave: Biotech; Bioengineering; Biotechnology; Food labels; Auctions; Experimental economics; Random nth price;
Willingness to pay; Demand and Price Analysis; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/33577
BRAZIL: THE FUTURE OF MODERN AGRICULTURE?
Provedor de dados: 31
Autores: Matthey,
Holger; Fabiosa,
Jacinto F.; Fuller,
Frank H.
In an attempt to understand better Brazil'’ s future role in agricultural markets, the authors of this report traveled to Brazil on a
fact-finding mission in September 2003. The goal was to get a first-hand impression of Brazil’ 's agricultural sector and especially
its future potential. In this report we provide a general description of crop and livestock production, government policies, public
and private cooperation, and transportation and biotechnology issues. The most striking observations made during our time in
Brazil was the universal sense of optimism expressed by producers, government officials, agronomists, and market analysts about
the future growth of Brazilian agricultural production. Does that mean Brazil, as one person suggested, is the...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural development; Agricultural policy; Biotechnology; Infrastructure; Brazil; Production; Agricultural
and Food Policy; International Development; Production Economics; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2004
URL: http://purl.umn.edu/18705
Product Life Cycles and Innovation in the US Seed Corn Industry
Provedor de dados: 31
Autores: Magnier,
Alexandre; Kalaitzandonakes,
Nicholas G.; Miller,
Douglas J.
The purpose of this study is to evaluate potential changes in the length of product life cycles in the US seed corn industry. We use
the observed survival time on the market for hybrids sold during 1997-2009 to conduct a survival analysis. Our empirical results
show that the expected lifetimes for corn hybrids with single biotech traits are 5-15% longer than for hybrids with multiple
(stacked) traits, and the expected lifetimes for conventional corn are 13-17% longer than stacked hybrids. Also, the product life
cycles for all types of hybrids have decreased over the past twelve years (especially after 2004), but the rate of decline is roughly
similar across hybrid types. Based on this evidence, we conclude that the shorter product life cycles are closely...
Tipo: Journal Article
Palavras-chave: Accelerated failure time; Biotechnology; Product life cycle; Survival analysis; Productivity Analysis; Research
and Development/Tech Change/Emerging Technologies; Research Methods/ Statistical Methods; Risk and Uncertainty.
Ano: 2010
URL: http://purl.umn.edu/93557
Engineering Resistance and Disease Management in ornamental Crops
Provedor de dados: 185
Autores: Hei-ti Hsu.
[[abstract]]Viral diseases of ornamental plants cause major losses in productivity and quality. Host pl1ant resistance offers an
effective means of controlling plant diseases caused by viruses. It minimizes the necessity for the application of pesticides.
However, there are many ornamentals in which no natural disease resistance is available. Genetic engineering allows the
introduction of specific, in some instances broad spectrum , disease resistance derived from other species , or even from the
pathogen it self into plant genotypes that have been selected for desirable horticultural properties.
Palavras-chave: Agricultural biotechnology; Floriculture; Genetic enhacement; Molecular breeding; Virus resistance Ecological
and Environmental Biosafety of Transgenic Plants : proceedings of international symposium [[classification]]9.
Ano: 2006
The Road to Commercialization of Transgenic Products in Taiwan -A Bridge Too Far
Provedor de dados: 185
Autores: Wen-Yen Kao.
[[abstract]]The government of Taiwan commenced a national effort of investing in biotechnology very early in the 1980s and
Steadily increased public investment in various national initiatives and programs. Today, after 10 years of the National Science
and Technology Program for Agricultural Biotechnology (NSTP/AB), it has established a solid research foundation, yet has
achieved no notable successes in its goals of commercialization. While no one has any doubts over the technological capacity that
Taiwan possesses, there are clearly gaps yet to be identified that are preventing the access of bridging R&D and markets.
This paper discusses strategies in product development cycles and possible deficiencies that Taiwan must overcome to realize this
great...
Palavras-chave: Agricultural biotechnology; Industrialization platform; Genetically modified organisms (GMOs); Regulatory
science; Capacity building Ecological and Environmental Biosafety of Transgenic Plants : proceedings of international
symposium [[classification]]9.
Ano: 2006
Immediate and long-term effects of polysaccharides-based formulations on human skin
Provedor de dados: 60
Autores: Camargo Junior,Flavio Bueno de; Gaspar,Lorena Rigo; Campos,Patrícia Maria Berardo Gonçalves Maia.
A new trend in cosmetic formulations is the use of biotechnological raw materials as the polysaccharides from Klebsiella
pneumoniae, which are supposed to enhance cell renewal, improve skin hydration and micro-relief. Botanical extracts of Myrtus
communis leaves contain different sugars, which may provide the same benefits. Thus, the objective of this study was to evaluate
through objective and subjective analysis the immediate and long-term effects of cosmetic formulations containing
polysaccharides biotechnologically-originated and / or the ones contained in Myrtus communis extracts. Three
polysaccharide-based and placebo formulations were applied on the forearm skin of 40 volunteers. Skin hydration, transepidermal
water loss (TEWL), viscoelasticity and...
Tipo: Journal article
Palavras-chave: Polysaccharides/cosmetic use; Klebsiella pneumoniae/cosmetic use; Myrtus communis/cosmetic use; Cosmetic
formulations/evaluation; Human skin/hydration; Cosmetology/use of biotechnology; Biotecnological raw materials/use in
cosmetology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300022
El sur del cambio en el plan de desarrollo del Ecuador 2007-2010 (Coyuntura)
Provedor de dados: 122
Autores: Ramírez Gallegos, René.
Metafórica y popularmente se entiende por “definir el norte” de un cambio significa clarificar aquel punto cardinal que marca de
manera incuestionable el camino a seguir, la hoja de ruta en pos de la transformación. Empero, ¿qué significaría dilucidar el “sur”
del cambio? A la cuestión podríamos aventurar algunas respuestas. Hallar “el sur” sería encontrar, a partir de la lectura crítica de
las formas dominantes de interpretación de la realidad, las otras modalidades que han permanecido ocultas, opacas,
invisibilizadas. De la misma manera, descubrir “el sur” del cambio implicaría hallarlo desde una posición particular, desde un
lugar específico, el de los que están “fuera” de los espacios de dominio, de los que no aceptan el sometimiento y resisten,...
Tipo: Artículo
Palavras-chave: PLAN DE DESARROLLO; PLAN NACIONAL; DEMOCRACIA; ENFOQUE; ENERGÍA; SOFTWARE;
BIOTECNOLOGÍA; PARQUE NACIONAL YASUNÍ; TECNOLOGÍA; DIGNIDAD; DESARROLLO; ECUADOR;
DEVELOPMENT PLAN; NATIONAL PLAN; DEMOCRACY; APPROACH; ENERGY; SOFTWARE; BIOTECHNOLOGY;
YASUNI NATIONAL PARK; TECHNOLOGY; DIGNITY; DEVELOPMENT.
Ano: 2007
URL: http://hdl.handle.net/10469/4830
Genetically Modified Crops: Risks and Promise
Provedor de dados: 7
Autores: Conway, Gordon; The Rockefeller Foundation; [email protected].
GM foods have the potential to provide significant benefits for developing countries. Over 800 million people are chronically
undernourished, and 180 million children are severely underweight for their age. By 2020, there will be an extra two billion
mouths to feed. Ecological approaches that underpin sustainable agriculture (e.g., integrated pest management) and participatory
approaches that strengthen farmers' own experimentation and decision making are key. Biotechnology will be an essential partner,
if yield ceilings are to be raised, if crops are to be grown without excessive reliance on pesticides, and if farmers on less favored
lands are to be provided with crops that are resistant to drought and salinity, and that can use nitrogen and other...
Tipo: Peer-Reviewed Reports
Palavras-chave: Bacillus thuringiensis (B.t.); Food security; Genetically modified rice; GM foods; Intellectual property rights;
Marker-aided selection; Monsanto; Participatory approaches; Plant biotechnology; Plant variety protection; Terminator
technology; Vitamin A deficiency.
Ano: 2000
Rethinking the Risk Management Process for Genetically Engineered Crop Varieties in Small-scale,
Traditionally Based Agriculture
Provedor de dados: 7
Autores: Cleveland, David A; University of California, Santa Barbara; [email protected]; Soleri, Daniela; University of
California, Santa Barbara; [email protected].
Tipo: Peer-Reviewed Reports
Palavras-chave: Agricultural biotechnology; Biological diversity; Biological invasion; Crop genetic resources; Farmer
participation in risk evaluation; Gene flow; Genetic engineering; Risk analysis; Risk management process; Traditionally based
agricultural systems compared with industrial agriculture systems; Transgenes; Transgenic crop varieties.
Ano: 2005
Uso de marcadores RAPD e do sistema de informacao geografica no estudo da variabilidade genetica
de Stylosanthes macrocephala.
Provedor de dados: 14
Autores: BARROS, A. M.; FALEIRO, F. G.; SHIRATSUCHI, L. S.; KARIA, C. T.; LOPES, G. K. B.; ANDRADE, R. P.
0
Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E)
Palavras-chave: Marcador molecular; RAPD; Variacao genetica; Stylosanthes macrocepala; Leguminosa forrageira;
Biogeografia; Sistema de informacao geografica; Marcador genetico; Biotecnologia; Genetic markers; Genetic variation; Feed
legumes; Biogeography; Geographic information systems; Molecular biology; Biotechnology.
URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567934
Biotechnology and Planted Forests: Assessment of Potential and Possibilities
Provedor de dados: 31
Autores: Sedjo,
Roger A.
This paper addresses the potential impact of the introduction and development of biotechnology on planted forests. It includes a
description of some recent innovations in forestry including the use of traditional breeding, and also more recent innovations
involving biotechnology, including the development of clonal propagation and the use of modern molecular biology techniques. In
addition to describing these innovations, the paper undertakes an assessment of their probable impact on future production of the
forest industry, on the global timber supply, and on future markets for timber and wood products. The paper offers a description of
recent innovations in tree breeding and biotechnology, including a discussion of innovations in agriculture that have...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Plantation forests; Genetic modification; Genetic research; Economic benefits; Transgenic;
GMO; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 1999
URL: http://purl.umn.edu/10862
Assessment of Biotechnology Policies and International Trade in Key Markets for U.S. Agriculture
Provedor de dados: 31
Autores: Marchant,
Mary A.; Song,
Baohui.
The United States leads the world in agricultural biotechnology research, adoption, commercialization, and exports. Our biotech
commodities are highly dependent on international markets. Thus, any biotech policy changes by key importing countries may
affect U.S. agricultural biotech product exports. This article identifies key markets for U.S. agricultural exports including biotech
commodities and discusses current and proposed biotech policies in key markets for U.S. agricultural exports focusing on Canada,
Mexico, Japan, the European Union (EU), and China. Among these markets, labeling of biotech products is voluntary in Canada
and Mexico but is mandatory in Japan, the EU, and, most recently, in China. For the EU, U.S. corn exports were almost...
Tipo: Journal Article
Palavras-chave: Biotechnology; Biotech policy; Corn; Cotton; International trade; Soybeans; U.S. agricultural exports; Q13;
Q17; Q16; Q18.
Ano: 2005
URL: http://purl.umn.edu/43513
Animal Disease and the Industrialization of Agriculture
Provedor de dados: 31
Autores: Hennessy,
David A.; Wang,
Tong.
The industrialization of animal agriculture has fundamentally transformed animal health markets while animal health innovations
have promoted this industrialization. The subtlety of these interactions shows how little we know about agricultural
industrialization. To illustrate, we consider three stylized features of industrialized animal agriculture. These are the closing off of
production activities from external effects, emphasis on control, and use of biosecurity measures. We find that animal disease
externalities should lead to higher stocking on any given farm, and also to deficient entry into animal production. Eradicating the
disease in a region increases both the stocking rate per farm and the number of farms. We show that antibiotics as a control...
Tipo: Working or Discussion Paper
Palavras-chave: Animal disease; Biosecurity; Biotechnology; Competitiveness; Confined animal agriculture; Economies of
scale; Tragedy of the commons; Veterinary inputs.; Agricultural and Food Policy; Livestock Production/Industries; Production
Economics; Research and Development/Tech Change/Emerging Technologies.
Ano: 2010
URL: http://purl.umn.edu/93673
PUBLIC-PRIVATE PARTNERSHIPS IN AGRICULTURAL RESEARCH: AN ANALYSIS OF
CHALLENGES FACING INDUSTRY AND THE CONSULTATIVE GROUP ON
INTERNATIONAL AGRICULTURAL RESEARCH
Provedor de dados: 31
Autores: Spielman,
David J.; von Grebmer,
Klaus.
Public-private partnerships offer potentially important opportunities for pro-poor agricultural research in developing countries.
Yet in the international agricultural research community--and with regard to the Consultative Group on International Agricultural
Research (CGIAR) itself--we see few examples of successful public-private partnerships, and fewer examples where such
collaborations have contributed to food security, poverty reduction and economic growth. This study assesses the opportunities
for, and challenges to, creating and sustaining public-private partnerships between the international agricultural research centers of
the CGIAR and leading multinational, research-based agribusiness companies. The study hypothesizes that the willingness and...
Tipo: Working or Discussion Paper
Palavras-chave: Public-private partnership; Collaborative research; Private sector; Multinational firms; Agricultural research and
development; Agricultural biotechnology; Intellectual property rights; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2004
URL: http://purl.umn.edu/16089
National Agricultural Biotechnology Research Capacity in Developing Countries
Provedor de dados: 31
Autores: Cohen,
Joel I.; Komen,
John; Zepeda,
Jose Falck.
Adequate public research capacity is key to the appropriate development of biotechnology, including genetically modified (GM)
crops. While commercial crops can be introduced without intensive local research (i.e. insect resistant GM cotton), introducing
products of public research depend on indigenous capacity. This paper defines capacity for agricultural biotechnology research
and then provides national funding levels for such work in six developing countries. As one indicator of capacity and outputs, GM
crops developed from public research in developing countries are documented, and attention given to issues remaining for
capacity, research and development. Knowledge of investments in public biotechnology improves policy decisions, clarifies roles
of the...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Research Capacity; GMO; Biosafety; Investments; Funding.; Research and Development/Tech
Change/Emerging Technologies; O31; O38; Q16.
Ano: 2004
URL: http://purl.umn.edu/23790
Current Contribution of Four Biotechnologies to New Zealand's Primary Sector
Provedor de dados: 31
Autores: Kaye-Blake,
William; Saunders,
Caroline M.; Emanuelsson,
Martin.
Over the last several decades, changes to production technologies have significantly increased output from the primary sector. The
contribution of improved genetics has been variously estimated, and the impacts of genetic engineering have received much
attention. The present research estimated the economic impact of four modern biotechnologies (other than genetic engineering) on
the primary sector. Information from interviews with key informants was incorporated into a cost-benefit analysis to estimate
these impacts. The net contribution was estimated at $266 million per year, with impacts spread unevenly through the sector and
across biotechnologies.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Cost-benefit analysis; Marker assisted selection; New Zealand; Primary production; Research
and Development/Tech Change/Emerging Technologies; O30; Q16.
Ano: 2006
URL: http://purl.umn.edu/25411
FORCES AFFECTING CHANGE IN CROP PRODUCTION AGRICULTURE
Provedor de dados: 31
Autores: Bechdol,
Elizabeth; Gray,
Allan W.; Gloy,
Brent A.
Tipo: Journal Article
Palavras-chave: Agricultural Competitiveness; Crop Production; Agricultural Biotechnology; Agricultural Sustainability;
Agricultural Inputs; Agricultural Profitability; Crop Production/Industries; Q10; Q12; Q13.
Ano: 2010
URL: http://purl.umn.edu/100787
Endogenous R&D Investment and Market Structure: A Case Study of the Agricultural
Biotechnology Industry
Provedor de dados: 31
Autores: Anderson,
Benjamin; Sheldon,
Ian M.
Over the past three decades, the agricultural biotechnology sector has been characterized by rapid innovation, market
consolidation, and a more exhaustive definition of property rights. The industry attributes consistently identified by the literature
and important to this analysis include: (i) endogenous sunk costs in the form of expenditures on R&D; (ii) seed and
agricultural chemical technologies that potentially act as complements within firms and substitutes across firms; and (iii) property
rights governing plant and seed varieties that have become more clearly defined since the 1970s. This paper adds to the stylized
facts of the agricultural biotechnology industry to include the ability of firms to license technology, a phenomenon observed
only...
Tipo: Conference Paper or Presentation
Palavras-chave: Licensing; Market structure; R&D; Agricultural biotechnology; Research and Development/Tech
Change/Emerging Technologies; L22; L24; Q16.
Ano: 2011
URL: http://purl.umn.edu/107832
Effects of Seed and Farm Characteristics on Cottonseed Choice: A Choice-Based Conjoint
Experiment in the Mississippi Delta
Provedor de dados: 31
Autores: Banerjee,
Swagata (Ban); Hudson,
Darren; Martin,
Steven W.
Producers’ preferences for cottonseed with respect to price, seed type, yield, and fiber quality are examined by a
willingness-to-pay approach via mail surveys. Results indicate a positive willingness to pay (WTP) for technology relative to
conventional cottonseed, and WTP increases with the level of technology. Yield and quality also show a positive WTP. Larger
farms have a higher WTP for technology, and farms with more farm labor have a lower WTP for technology. These results
suggest economies of size in technology adoption (biotechnology is not size-neutral) and that labor and biotechnology are direct
substitutes.
Tipo: Journal Article
Palavras-chave: Biotechnology; Conjoint analysis; Conjoint (choice) experiment; Cotton; Farm labor; Farm size; Fiber quality;
Willingness to pay (WTP); D24; Q12; Q16.
Ano: 2007
URL: http://purl.umn.edu/37054
SIZE AND DISTRIBUTION OF MARKET BENEFITS FROM ADOPTING BIOTECH CROPS
Provedor de dados: 31
Autores: Price,
Gregory K.; Lin,
William W.; Falck-Zepeda,
Jose Benjamin; Fernandez-Cornejo,
Jorge.
This study estimates the total benefit arising from the adoption of agricultural biotechnology in one year (1997) and its
distribution among key stakeholders along the production and marketing chain. The analysis focuses on three biotech crops:
herbicide-tolerant soybeans, insect-resistant (Bt) cotton, and herbicide-tolerant cotton. Adoption of these crops resulted in
estimated market benefits of $212.5-$300.7 million for Bt cotton, $231.8 million for herbicide-tolerant cotton, and $307.5 million
for herbicide-tolerant soybeans. These benefits accounted for small shares of crop production value, ranging from 2 percent to 5
percent. U.S. farmers captured a much larger share (about a third) of the benefits for Bt cotton than with herbicide-tolerant
soybeans...
Tipo: Report
Palavras-chave: Agricultural biotechnology; Distribution of benefits; Bt cotton; Herbicide-tolerant cotton; Herbicide-tolerant
soybeans; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/33562
Biotechnology's Potential Contribution to Global Wood Supply and Forest Conservation
Provedor de dados: 31
Autores: Sedjo,
Roger A.
Over the past 30 years, industrial plantation forests have become a major supplier of industrial wood. There are several reasons for
this, including the improved economics of planted forests due to biotechnological innovations, the increases in natural forest wood
costs due to increasing inaccessibility, and rising wood costs from natural forests due to new environmental restrictions related to
logging. Forestry today is on the threshold of the widespread introduction of biotechnology into its operational practices. In many
cases, the biotechnology likely to be introduced is simply an extension of that being utilized in agriculture, such as
herbicide-tolerant genes. However, biotechnology in forestry also is developing applications unique to forestry,...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Breeding; Forestry; Tree plantations; Timber; Fiber; Genes; GMOs; Industrial wood;
Economics; Benefits; Costs; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2001
URL: http://purl.umn.edu/10708
IS SOY MILK? THE ECONOMICS OF THE SOY MILK MARKET
Provedor de dados: 31
Autores: Dhar,
Tirtha Pratim; Foltz,
Jeremy D.
This study uses revealed preferences of consumers to study the consumer benefits from soy milk. The study specifies and
estimates structural demand and reduced form models of competition for different milk types using US supermarket scanner data.
The introduction of soy milk is used to estimate consumer benefits and valuations. We decompose benefits into two components,
competitive and variety effects. Results show relatively small consumer benefits from soy milk.
Tipo: Conference Paper or Presentation
Palavras-chave: Demand Systems; Q-AIDS; Milk markets; Biotechnology; Soy milk; Food Labeling; Demand and Price
Analysis; Q130; C300; D120; D400.
Ano: 2004
URL: http://purl.umn.edu/20337
Agricultural Biotechnology and the FTAA: Issues and Opportunities
Provedor de dados: 31
Autores: Gaisford,
James D.
This article examines trade and intellectual property rights questions associated with agricultural biotechnology in the Western
Hemisphere and goes on to chart a potential course through which they could be addressed by an agreement on a Free Trade Area
of the Americas. Issues pertaining to import approvals, labelling, exports to sensitive markets, intellectual property piracy and
regulatory cooperation are considered.
Tipo: Journal Article
Palavras-chave: Biotechnology; BioSafety Protocol; Free Trade Area of the Americas; FTAA; World Trade Organization;
International Relations/Trade; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/23926
Have Biotech Seeds Increased Maize Yields?
Provedor de dados: 31
Autores: Xu,
Zheng; Hennessy,
David A.; Moschini,
GianCarlo.
Corn yield is determined by soils, weather, seed used and other technology choices. Global population and per capita income
growth trends as well as demand from the energy sector have placed great stress on cropland use. Global cropland acres and/or
yield per acre will need to increase. Whether new seed technologies have enhanced corn yield is a controversial issue. We study
U.S. county corn yields 1964-2008, controlling for location effects, fertilization technologies and weather. We find evidence that
trend yield growth has been fastest in the Central Corn Belt, genetic modification technologies have increased trend yield, and this
increase has been largest in the Central Corn Belt.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Corn Yield; Trend; Regional Effects; Weather; Fertilization.; Crop Production/Industries;
Industrial Organization; Research and Development/Tech Change/Emerging Technologies; L65; Q16.
Ano: 2010
URL: http://purl.umn.edu/61303
ADOPTION OF BIOENGINEERED CROPS
Provedor de dados: 31
Autores: Fernandez-Cornejo,
Jorge; McBride,
William D.
Use of crop biotechnology products, such as genetically engineered (GE) crops with input traits for pest management, has risen
dramatically since commercial approval in the mid-1990s. This report addresses several of the economic dimensions regarding
farmer adoption of bioengineered crops, including herbicidetolerant and insect-resistant varieties. In particular, the report
examines: (1) the extent of adoption of bioengineered crops, their diffusion path, and expected adoption rates over the next few
years; (2) factors affecting the adoption of bioengineered crops; and (3) farm-level impacts of the adoption of bioengineered
crops. Data used in the analysis are mostly from USDA surveys.
Tipo: Report
Palavras-chave: Biotechnology; Technology adoption; Genetic engineering; Pest management; Financial effects; Tillage;
Herbicide-tolerant crops; Bt crops; Corn; Soybeans; Cotton; Crop Production/Industries; Research and Development/Tech
Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/33957
MANAGING PEST RESISTANCE: THE POTENTIAL OF CROP ROTATIONS AND
SHREDDING
Provedor de dados: 31
Autores: Babcock,
Bruce A.; Secchi,
Silvia.
The current debate over resistance management plans mandated by the Environmental Protection Agency for transgenic crops
ignores management practices that are complementary to refuge schemes. A dynamic production model is developed that
measures the costs and benefits of crop rotation and shredding in terms of delaying resistance to Bt corn.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Common property resource; Crop rotation; European Corn Borer; Externalities; Insecticide
resistance; Integrated pest management; Intertemporal optimization; Crop Production/Industries; Research and Development/Tech
Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21597
Private Research and Public Goods: Implications of Biotechnology for Biodiversity
Provedor de dados: 31
Autores: Raney,
Terri; Pingali,
Prabhu L.
The pattern of crop genetic diversity has changed over the past two centuries with the modernization of agriculture, accelerating
with the advent of the green revolution. Since the green revolution, the locus of agricultural research has shifted from the public to
the private sector. The growing importance of the private sector in agricultural R&D is changing the types of crop
technologies that are developed and the ways they are delivered to farmers. The spread of transgenic crops will influence crop
genetic diversity, but their implications for the availability of plant genetic resources and the resilience of agricultural ecosystems
are not entirely clear. Transgenic crops may increase or decrease crop genetic diversity, depending on how they are...
Tipo: Working or Discussion Paper
Palavras-chave: Biodiversity; Biotechnology; Agricultural Research; Transgenic Crops; Green Revolution.; O13; O33; Q16;
Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/23805
The Economic Impacts of Biotechnology-Based Technological Innovations
Provedor de dados: 31
Autores: Traxler,
Greg.
Global adoption of transgenic crops reached 67.7 million hectares in 2003 from 2.8 million in 1996. Delivery has occurred almost
entirely through the private sector and adoption has been rapid in areas where the crops addressed serious production constraints
and where farmers had access to the new technologies. Three countries (USA, Argentina and Canada), three crops (soybean,
cotton and maize) and two traits (insect resistance and herbicide tolerance) account for the vast majority of global transgenic area.
While some farmers in some developing countries are benefiting, most do not have access to transgenic crops and traits that
address their needs. This paper surveys the level and distribution of the economic impacts of transgenic cotton and soybeans to...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural Biotechnology; Economic Development; Technological Change; Cotton; Soybean; Research and
Development/Tech Change/Emerging Technologies; O13; Q12; Q16.
Ano: 2004
URL: http://purl.umn.edu/23806
Market Impacts of Adopting Herbicide-Resistant Rice in the Southern United States
Provedor de dados: 31
Autores: Fuller,
Frank H.; Annou,
Mamane Malam; Wailes,
Eric J.
Herbicide-resistant (HR) rice varieties offer U.S. rice producers a powerful tool for control of red rice infestations. However,
improved weed control can shorten crop rotations and boost yields, resulting in expanded rice production and lower domestic
market prices. Declining market returns diminish the benefits of HR rice adoption and substantially reduce net returns for
nonadopters. More competitive prices increase U.S. rice exports, causing a slight decline in world rice prices. The dependence
of the rice marketing loan program on world prices prevents loan deficiency payments from adequately offsetting producers’
market revenue losses. U.S. consumers gain from lower rice prices.
Tipo: Journal Article
Palavras-chave: Biotechnology; Crop rotation; Herbicide resistant; Red rice; Rice; Simulation models; Technology adaptation;
Q11; Q16; Q18; O33; C53.
Ano: 2003
URL: http://purl.umn.edu/37859
CREATING, PROTECTING, AND USING CROP BIOTECHNOLOGIES WORLDWIDE IN AN
ERA OF INTELLECTUAL PROPERTY
Provedor de dados: 31
Autores: Pardey,
Philip G.; Koo,
Bonwoo; Nottenburg,
Carol.
Proponents tout the positive incentive-to-innovate effects of intellectual property rights (IPRs), while others maintain that the
expanding subject matter and geographical extent of IPRs are stifling crop research, especially research and development
(R&D) dealing with developing-country crop concerns.
Much of this debate relies on anecdotes and misleading or
incomplete evidence on the extent and nature of the IPRs pertaining to crop technologies, including the jurisdictional extent of the
property rights and their practice. In this paper we review the evidence on the scope of agricultural R&D worldwide,
provide new data on the structure of crop-related IPRs, and summarize trends on the uptake of proprietary bioengineered crops.
Tipo: Working or Discussion Paper
Palavras-chave: Plant patents; Utility patents; Plant breeders' rights; Crop varieties; Public and private agricultural R&D;
Biotechnology; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/13600
GENETICALLY MODIFIED CROP INNOVATIONS AND PRODUCT DIFFERENTIATION:
TRADE AND WELFARE EFFECTS IN THE SOYBEAN COMPLEX
Provedor de dados: 31
Autores: Sobolevsky,
Andrei; Moschini,
GianCarlo; Lapan,
Harvey E.
We develop a new partial equilibrium, four-region world trade model for the soybean complex comprising soybeans, soybean oil,
and soybean meal. In the model, some consumers view genetically modified Roundup Ready (RR) soybeans and products as
weakly inferior to conventional ones; the RR seed is patented and sold worldwide by a U.S. firm; and producers employ a costly
segregation technology to separate conventional and biotech products in the supply chain. The calibrated model is solved for
equilibrium prices, quantities, production patterns, trade flows, and welfare changes under different assumptions regarding
regional government's production and trade policies, differentiated consumer tastes, and several other demand and supply
parameters. Incomplete...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Differentiated demand; Food labeling; Genetically modified products; Identity preservation;
Innovations; Intellectual property rights; International trade; Loan deficiency payments; Market failure; Monopoly; Roundup
Ready soybeans; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/18348
SOCIO-ECONOMIC IMPACT OF BIOTECHNOLOGY APPLICATIONS: SOME LESSONS
FROM THE PILOT TISSUE-CULTURE (TC) BANANA PRODUCTION PROMOTION PROJECT
IN KENYA, 1997-2002
Provedor de dados: 31
Autores: Mbogoh,
Stephen G.; Wambugu,
Florence M.; Wakhusama,
Sam.
This article is based on a socio-economic impact study of the introduction and adoption of tissue-culture (tc) technology in banana
production in Kenya. It attempts to demonstrate that a prudent introduction and promotion of a new biotechnological innovation in
farming can make a positive contribution to the socio-economic status of resource poor farmers in a developing country, such as
Kenya. Adoption of tc technology in banana production in Kenya is considered a good example of biotechnological applications
in agriculture. Hence the article hopes to make a contribution to recent debates at international levels as to whether biotechnology
can make a difference in uplifting the living standards of people in the third world (Qaim, 1999; Graff, et al 2002;...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology and Tissue-Culture (tc) Banana; Adoption; Socio-economic Impact; Rural Development; Third
World; Kenya.; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/25929
Agricultural Technology Choices for Poor Farmers in Less-Favored Areas of South and East Asia
Provedor de dados: 31
Autores: Pender,
John L.
During the past several decades dramatic improvement has occurred in agricultural productivity and livelihoods in South and East
Asia, stimulated by the Green Revolution and supported by several other factors. Nevertheless, hundreds of millions of rural
people in less-favored environments of this region still live in poverty and received limited benefit from the Green Revolution. To
address these problems, alternative technological approaches to the conventional Green Revolution technologies are being
advocated to address the problems of poor farmers in less-favored areas of Asia, including low external input and sustainable
agricultural approaches, organic agriculture and biotechnology. This paper reviews the literature on agricultural technology
options...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural technologies; Low-external input and sustainable agriculture; Organic farming; Biotechnology;
Less favored areas; Rural poverty; South and East Asia; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/42404
CONSUMER VALUATION OF GENETICALLY MODIFIED FOODS AND THE EFFECT OF
INFORMATION BIAS
Provedor de dados: 31
Autores: Vanwechel,
Tamara; Wachenheim,
Cheryl J.; Schuck,
Eric C.; Lambert,
David K.
Bid prices were elicited for standard-label cookies, muffins, and potato chips and those identified as not including genetically
modified (GM) ingredients using an experimental auction. Including a statement that the product did not include GM ingredients
increased bids over those offered for standard-label products. Providing negative-biased information about the impact of GM
crops on the environment increased the risk participants associated with GM foods, and positive-biased information decreased
perceived risk.
Overall, providing impact information, whether positive- or negative-biased, increased bids for products
presumed GM. The influence of information bias on bids varied among selected participant groups, supporting the presence of
uniquely...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Environment; Experimental auction; Genetically modified; Information; Willingness-to-pay;
Food Consumption/Nutrition/Food Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/23620
Research Investments and Market Structure in the Food Processing, Agricultural Input, and Biofuel
Industries Worldwide
Provedor de dados: 31
Autores: Fuglie,
Wang,
Keith O.; Heisey,
Paul W.; King,
John L.; Day-Rubenstein,
Kelly A.; Schimmelpfennig,
David E.;
Sun Ling.
Meeting growing global demand for food, fiber, and biofuel requires robust investment in agricultural research and development
(R&D) from both public and private sectors. This study examines global R&D spending by private industry in seven
agricultural input sectors, food manufacturing, and biofuel and describes the changing structure of these industries. In 2007 (the
latest year for which comprehensive estimates are available), the private sector spent $19.7 billion on food and agricultural
research (56 percent in food manufacturing and 44 percent in agricultural input sectors) and accounted for about half of total
public and private spending on food and agricultural R&D in high-income countries. In R&D related to biofuel,
annual...
Tipo: Technical Report
Palavras-chave: Agricultural biotechnology; Agricultural chemicals; Agricultural inputs; Animal breeding; Animal health;
Animal nutrition; Aquaculture; Biofuel; Concentration ratio; Crop breeding; Crop protection; Farm machinery; Fertilizers;
Herfindahl index; Globalization; Market share; Market structure; Research intensity; Seed improvement; Productivity Analysis.
Ano: 2011
URL: http://purl.umn.edu/120324
Crisis as a catalyst: the role of Schumpeterian innovation in the Lithuanian economy
Provedor de dados: 31
Autores: Giedraitis,
Vincentas Rolandas; Rasteniene,
Ausra.
What circumstances allow businesses to flourish in a stagnant world economy? We ask that question in our discussion of the
uniquely favorable circumstances of the biotechnology sector in Lithuania. The purpose of this paper is to analyze Lithuania’s
ability to expand its economy during a time of crisis, focusing on its unique ability to innovate in such sectors as biotechnology.
Tipo: Journal Article
Palavras-chave: Schumpeter; Innovation; Kondratiev wave; Biotechnology; Business cluster theory; Financial Economics;
International Development; Research and Development/Tech Change/Emerging Technologies; O31; O32.
Ano: 2009
URL: http://purl.umn.edu/94527
RGDU06337-MAS-24-B-B: ข้ าวเจ้ าอายุเบาสาหรับนานา้ ฝนภาคตะวันออกเฉียงเหนือ
Provedor de dados: 106
Autores: Somjai Saleeto.
The program on research and development of rainfed lowland rice in north eastern Thailand in collaboration with the National
Centre for Genetic Engineering and Biotechnology (BIOTEC) have carried out the project to develop an early maturing, blast
resistant, non-glutinous rice variety. The cross between IR57514-PMI-5-B-1-2 and RGDU99016-MAS-77 was developed and the
progenies were backcrossed to IR57514-PMI-5-B-1-2 for 3 time at RGDU, BIOTEC. The selected promising line;
RGDU06337-MAS-24-B-B, was finally obtained. This line is a non-glutinous, photoperiod sensitive, early maturing line which
flowers approximately on October 8 and is ready for harvest on November 11. The average number of panicles per hill is 11
panicles. The average plant height is 114 cm....
Tipo: PhysicalObject
Palavras-chave: Rice; Non-glutinous rice; Rainfed lowland rice; Semi dwarf; Blast; Blast resistance; Early maturing rice;
North-eastern region; Biotechnology; Genetic engineering; ข้าว; ข้าวนาน้ าฝน; ต้นเตี้ย; โรคไหม้; ความต้านทานต่อโรคไหม้; ข้าวเบา; ข้าวอายุส้ นั ;
ภาคตะวันออกเฉี ยงเหนือ; เทคโนโลยีชีวภาพ; พันธุวศิ วกรรม.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5631
Interferências no agrossistema e riscos ambientais de culturas transgênicas tolerantes a herbicidas e
protegidas contra insetos.
Provedor de dados: 119
Autores: SIQUEIRA, J.O.; TRANNIN, I.C. de B.; RAMALHO, M.A.P.; FONTES, E.M.G.
A engenharia genética possibilitou o desenvolvimento de cultivares transgênicas, cuja área plantada em 18 países atinge 67,7
milhões de hectares, principalmente de cultivares tolerantes a herbicidas e protegidas contra insetos-praga. Mesmo após rigorosa
avaliação de riscos, o plantio dessas cultivares tem despertado preocupação com impactos ambientais, como escape gênico e
efeitos na biodiversidade. Considerando o milho e a soja, o risco de fluxo gênico horizontal é remoto pela inexistência de espécies
silvestres compatíveis, existindo esse risco para algodão em certas regiões do Brasil. O escape gênico para lavouras convencionais
pode ocorrer, mas pode ser evitado por isolamento dos cultivos. Outra preocupação é o impacto de cultivares expressando...
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Organismos geneticamente modificados (OGMs); Biotecnologia agrícola; Plantas transgênicas; Biossegurança;
Impacto ambiental; Proteínas Cry; Tolerância a herbicida; Organismos do solo; Entomofauna; Fluxo gênico; Genetically modified
organisms (GMOs); Agricultural biotechnology; Transgenic plants; Biosafety; Environmental impact; Cry proteins; Herbicide
tolerance; Soil organisms; Entomofauna; Gene flow.
Ano: 2004
URL: http://www.alice.cnptia.embrapa.br/handle/doc/110861
Rural Poverty and Employment Effects of Bt Cotton in India
Provedor de dados: 31
Autores: Subramanian,
Arjunan; Qaim,
Matin.
The impact of genetically modified (GM) crops on the poor in developing countries is still the subject of controversy. While
previous studies have examined direct productivity effects of Bacillus thuringiensis (Bt) cotton and other GM crops, little is
known about wider socioeconomic outcomes. We use a microeconomic modelling approach and comprehensive survey data from
India to analyze welfare and distribution effects in a typical village economy. Bt cotton adoption increases aggregate employment
with interesting gender implications. Likewise, aggregate household incomes rise, including for poor and vulnerable farmers,
highlighting that Bt cotton contributes to poverty reduction and rural development.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Genetically modified crops; Poverty; Rural development; Village economy; Social accounting
matrix; Impacts; Agricultural and Food Policy; Community/Rural/Urban Development; Crop Production/Industries; Food Security
and Poverty; International Development; Production Economics; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2009
URL: http://purl.umn.edu/50555
Designing Regional Systems of Biotechnology Regulation A Transaction Cost Approach to Regulatory
Governance
Provedor de dados: 31
Autores: Birner,
Regina; Linacre,
Nicholas A.
Many developing countries are currently in the process of designing regulatory systems that should make it possible to use the
potential of genetically modified organisms (GMOs) for agricultural development, while at the same time managing the risks for
food safety and the environment that are potentially associated with these technologies. In view of the considerable costs
associated with biotechnology regulation and the scarcity of biosafety specialists, there are processes in various regions of the
developing world to establish regional systems of biotechnology regulation. So far, there are major knowledge gaps as to how
regional systems of biotechnology regulation can be designed to be effective and efficient and to fulfill principles of good...
Tipo: Conference Paper or Presentation
Palavras-chave: Regional biotechnology regulation; Regulatory federalism; Transaction cost economics; European Union;
Agricultural and Food Policy; Community/Rural/Urban Development; Consumer/Household Economics; Environmental
Economics and Policy; Farm Management; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International
Relations/Trade; Marketing; Production Economics; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/52218
GM food technology abroad and its implications for Australia and New Zealand
Provedor de dados: 31
Autores: Anderson,
Kym; Jackson,
Lee Ann.
The potential economic benefits from agricultural biotechnology adoption by ANZ need to be weighed against any likely loss of
market access abroad for crops that may contain genetically modified (GM) organisms. This paper uses the global GTAP model to
estimate effects of other countries' GM policies without and with ANZ farmers adopting GM varieties of various grains and
oilseeds. The benefits to ANZ from adopting GM crops under a variety of scenarios are positive even in the presence of the ban on
imports from GM-adopting countries by the EU (but not if East Asia also applied such a ban).
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; GMOs; Regulation; Trade policy; Computable general equilibrium; Crop Production/Industries;
Food Consumption/Nutrition/Food Safety; C68; D58; F13; O3; Q17; Q18.
Ano: 2004
URL: http://purl.umn.edu/58365
The Asian Maize Biotechnology Network (AMBIONET): A Model for Strengthening National
Agricultural Research Systems
Provedor de dados: 31
Autores: Pray,
Carl E.
This report reviews the impacts of the Asian Maize Biotechnology Network (AMBIONET), organized by the International Maize
and Wheat Improvement Center (CIMMYT) with funding from the Asian Development Bank to strengthen the capacity of public
maize research institutions in China, India, Indonesia, Philippines, Thailand, and Vietnam to produce high-yielding, disease
resistant, stress tolerant maize cultivars. It was found that, during its lifetime (1998-2005), AMBIONET clearly benefited
researchers and institutions in participating countries, as well as CIMMYT. In addition, there was good progress toward
developing improved cultivars. Asian farmers are just beginning to gain from the work, but their future benefits will likely pay for
AMBIONET’s relatively...
Tipo: Report
Palavras-chave: Zea mays; Plant breeding; Biotechnology; Breeding methods; Research methods; Disease resistance; Yield
increases; Research institutions; China; India; Indonesia; Philippines; Thailand; Viet Nam; Asia; Crop Production/Industries;
Research and Development/Tech Change/Emerging Technologies; F30; F01.
Ano: 2006
URL: http://purl.umn.edu/56103
Effects of Bt Cotton in India During the First Five Years of Adoption
Provedor de dados: 31
Autores: Sadashivappa,
Prakash; Qaim,
Matin.
While previous research has analyzed the impacts of Bt cotton in India, most available studies are based on one or two years of
data only. We analyze the technology’s performance over the first five years of adoption, using panel data with three rounds of
observations. On average, Bt adopting farmers realize pesticide reductions of about 40%, and yield advantages of 30-40%. Profit
gains are in a magnitude of US $60 per acre. These benefits have been sustainable over time. Farmers’ satisfaction is reflected in a
high willingness to pay for Bt seeds. Nonetheless, in 2006 Indian state governments decided to establish price caps at levels much
lower than what companies had charged before. This intervention has further increased farmers’ profits, but the impact...
Tipo: Journal Article
Palavras-chave: Biotechnology; Bt cotton; Genetically modified crops; Farm survey; India; Seed markets; Technology adoption;
Willingness to pay (WTP); Environmental Economics and Policy; Research and Development/Tech Change/Emerging
Technologies; O32; O33; Q16; Q55; Q58.
Ano: 2009
URL: http://purl.umn.edu/49947
A Dynamic Decision Model of Technology Adoption under Uncertainty: Case of Herbicide-Resistant
Rice
Provedor de dados: 31
Autores: Annou,
Mamane Malam; Wailes,
Eric J.; Thomsen,
Michael R.
Herbicide-resistant (HR) rice technology is a potential tool for control of red rice in commercial rice production. Using an ex
ante mathematical programming framework, this research presents an empirical analysis of HR rice technology adoption under
uncertainty. The analysis accounts for stochastic germination of red rice and sheath blight to model a profit maximization
problem of crop rotation among HR rice, regular rice, and soybeans. The results demonstrate that risk attitudes and technology
efficiency determine adoption rates and optimal rotation patterns.
Tipo: Journal Article
Palavras-chave: Biotechnology; Herbicide resistance; Mathematical programming; Profit maximization; Rice; Risk; Rotation;
Technology; Adoption; Crop Production/Industries; Production Economics; Research and Development/Tech Change/Emerging
Technologies; Q16; Q18; O33; C61.
Ano: 2005
URL: http://purl.umn.edu/43724
The U.S. Seed Industry: An Exploration of Statistics Highlighting the Economic Activity of the U.S.
Row Crop Seed Industry
Provedor de dados: 31
Autores: Roucan-Kane,
Maud; Gray,
Allan W.
This report presents relevant statistics that highlight the economic activity of the U.S. seed industry. The focus of this report is on
the four main U.S. crops: corn, soybean, wheat, and cotton. The report contains three sections. The first is related to the U.S. seed
market’s size based on seed sales and expenditures. The second section examines industry investment in research and
development (R&D) activity in terms of both budget and human resources. The final section illustrates the impact of the seed
industry in terms of intellectual property development, improved productivity, and other benefits. This report focuses on using
publicly available data to examine these three areas. In addition, the report presents the results of a survey conducted...
Tipo: Working or Discussion Paper
Palavras-chave: Seed industry; Field crops; Biotechnology; Herbicide-tolerant crops; Bt crops; Corn; Soybeans; Cotton; Wheat.;
Agribusiness; L11; L16; L65; O33; O34; Q16.
Ano: 2009
URL: http://purl.umn.edu/52549
Issues, impacts, and prospects of the first transgenic crops tolerant to a herbicide. The case of
glyphosate-tolerant soybean in the USA
Provedor de dados: 31
Autores: Bonny,
Sylvie.
IAAE 2009 International Conference
Tipo: Conference Paper or Presentation
Palavras-chave: Genetically modified crop; GMO; Biotechnology; Agro-economic impact; Environmental impact;
Sustainability; Soybean; Pesticide; Weed; Prospects; Herbicide; Glyphosate; Agricultural and Food Policy; Crop
Production/Industries; Environmental Economics and Policy; Production Economics; Research and Development/Tech
Change/Emerging Technologies; Q55; O3; O13; O51.
Ano: 2009
URL: http://purl.umn.edu/51449
ECONOMIC IMPACTS OF GENETICALLY MODIFIED CROPS IN CHINA
Provedor de dados: 31
Autores: Huang,
Jikun; Hu,
Ruifa; van Meijl,
Hans; van Tongeren,
Frank W.
China has made a major investment in biotechnology research. Genetically modified (GM) cotton is widely adopted and the list
of GM technologies in trials is impressive. At the same time there is an active debate on when China should commercialize its
GM food crops. The overall goal of this paper is to provide an economy-wide assessment of these issues under various scenarios.
Based on a unique data from empirical micro-level study and field trial in China and a modified GTAP model, our results indicate
that the development of biotechnology has an important impact on China's production, trade and welfare. Welfare gains far
outweigh the public biotechnology research expenditures. Most gains occur inside China. Policy makers should put less weight
on...
Tipo: Conference Paper or Presentation
Palavras-chave: Economic impacts; Biotechnology; Genetically modified crops; China; Crop Production/Industries; Research
and Development/Tech Change/Emerging Technologies; C68; D58; F13; O33; Q17; Q18.
Ano: 2003
URL: http://purl.umn.edu/25883
Transgenic Trees: Implementation and Outcomes of the Plant Protection Act
Provedor de dados: 31
Autores: Sedjo,
Roger A.
The responsibility for protecting U.S. agriculture from pests and diseases is assigned by the Federal Plant Pest Act (FPPA) to the
Animal and Plant Health Inspection Service (APHIS) of the Department of Agriculture. The Plant Protection Act (Title 7 U.S.C.
Sections 7701 et seq.) gives Aphis statutory authority over genetically modified organisms (GMO), in effect assigning to APHIS a
related responsibility of determining whether a genetically altered plant, crop, or tree is likely to pose unacceptable risks to the
environment. Although APHIS has considerable experience with crop plants, it has only limited experience with trees. Yet the
possible benefits of applying genetic engineering to trees are substantial and include industrial wood production and...
Tipo: Working or Discussion Paper
Palavras-chave: Forestry; Biotechnology; Transgenic; Tree plantations; Timber supply; Genes; GMOs; Industrial wood;
Economics; Regulations; Costs; Benefits; Conservation; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2004
URL: http://purl.umn.edu/10629
The Economics of Biotechnology (Gmos) and the Need for A Regional Policy: The Case for COMESA
Countries
Provedor de dados: 31
Autores: Minde,
Isaac J.; Kizito,
Mazvimavi.
Many countries in the world have adopted genetically modified organisms as products that can have great beneficial impact on
agriculture, industry and trade. However, to date for the whole of Africa, only South Africa has commercialized genetically
modified organisms (GMOs). Realizing the high transactions costs—particularly in trade that may underlie different countries
having varying policy stances on biotechnology, COMESA (COMESA—Common Market for Eastern and Southern Africa, is a
regional grouping of 20 countries. In the context of this study, Tanzania is also included although it is not a COMESA member.
This is because it belongs to the Association for Strengthening Agricultural Research in Eastern and Central Africa (ASARECA)
of which nine countries...
Tipo: Conference Paper or Presentation
Palavras-chave: Biosafety; Biotechnology; Africa; Policy; Agricultural and Food Policy; Consumer/Household Economics;
Demand and Price Analysis; Environmental Economics and Policy; Food Consumption/Nutrition/Food Safety; Food Security and
Poverty; International Relations/Trade; Marketing; Productivity Analysis; Research and Development/Tech Change/Emerging
Technologies.
Ano: 2008
URL: http://purl.umn.edu/52150
Agricultural Resources and Environmental Indicators, 2006 Edition
Provedor de dados: 31
Autores: Wiebe,
Keith D.; Gollehon,
Noel R.
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Internet Explorer.
Tipo: Report
Palavras-chave: ERS; AREI; Agricultural economics; Natural resources; Land; Land use; Land values; Land ownership; Water
use; Irrigation; Water quality; Genetic resources; Biotechnology; Agricultural research; Agricultural productivity; Global
resources; Soil conservation; Soil erosion; Pest management; Nutrient management; Animal agriculture; Organic agriculture;
Conventional agriculture; Conservation policy; Land retirement; Working lands; Wetlands; Farmland protection; Environmental
quality; Farm structure; Farm ownership; Farm management decisions; Farm business; Environmental Economics and Policy;
Resource /Energy Economics and Policy.
Ano: 2006
URL: http://purl.umn.edu/7207