Fanconi anemia and RAD50 deficiency: genetic and functional
... FA cell lines that could not be assigned to one of the known groups were further classified as defective or proficient in FANCD2 monoubiquitination, a central process in the FA caretaker pathway. This allowed to classify these patient cell lines as upstream or downstream partners within the FA caret ...
... FA cell lines that could not be assigned to one of the known groups were further classified as defective or proficient in FANCD2 monoubiquitination, a central process in the FA caretaker pathway. This allowed to classify these patient cell lines as upstream or downstream partners within the FA caret ...
p53, the Cellular Gatekeeper Review for Growth and Division
... protein concentration, in some cells p53 probably also exists in a latent form, inactive for transcription. Under these conditions, the p53 protein must receive a signal or alteration to activate it to function. The upstream events or signals that flow to p53 are mediated by several stressful situat ...
... protein concentration, in some cells p53 probably also exists in a latent form, inactive for transcription. Under these conditions, the p53 protein must receive a signal or alteration to activate it to function. The upstream events or signals that flow to p53 are mediated by several stressful situat ...
Phylogenetic analysis of members of the genus Rickettsia using the
... To confirm the phylogenetic analysis previously inferred by comparison of the citrate synthase and rOmpA gene sequences (gltA and ompA, respectively), the rOmpB gene (ompB) of 24 strains of the genus Rickettsia was amplified and sequenced. rOmpB is an outer-membrane protein of high molecular mass, t ...
... To confirm the phylogenetic analysis previously inferred by comparison of the citrate synthase and rOmpA gene sequences (gltA and ompA, respectively), the rOmpB gene (ompB) of 24 strains of the genus Rickettsia was amplified and sequenced. rOmpB is an outer-membrane protein of high molecular mass, t ...
invited review
... VDR-marked genomic regions, i.e., 1,25(OH)2D3-inducible enhancers, towards the basal transcriptional machinery that has assembled on accessible transcription start sites (TSSs) (Carlberg and Campbell 2013). This assembly of enhancer and TSS regions with RNA polymerase II, other nuclear adaptor prote ...
... VDR-marked genomic regions, i.e., 1,25(OH)2D3-inducible enhancers, towards the basal transcriptional machinery that has assembled on accessible transcription start sites (TSSs) (Carlberg and Campbell 2013). This assembly of enhancer and TSS regions with RNA polymerase II, other nuclear adaptor prote ...
A survey on computational methods for enhancer and
... and negative examples for modeling the general features of enhancers and enhancer targets. As a result, different studies have used a variety of ways to define “gold-standard” enhancers and enhancer targets for training their methods. A lot of these “gold-standard” examples are either not experiment ...
... and negative examples for modeling the general features of enhancers and enhancer targets. As a result, different studies have used a variety of ways to define “gold-standard” enhancers and enhancer targets for training their methods. A lot of these “gold-standard” examples are either not experiment ...
ABSTRACT Title of Document: PROGRAMMED
... The PTC is located at the bottom of the large cleft on the interface side of the large subunit underneath the central protuberance (Polacek & Mankin, 2005; Rodnina et al, 2006). The acceptor ends of the tRNAs meet at the bottom of the funnel-shaped active site above the entrance to the peptide exit ...
... The PTC is located at the bottom of the large cleft on the interface side of the large subunit underneath the central protuberance (Polacek & Mankin, 2005; Rodnina et al, 2006). The acceptor ends of the tRNAs meet at the bottom of the funnel-shaped active site above the entrance to the peptide exit ...
factor involved in dorsal-ventral axis formation and neurogenesis
... indicated by the top line. Arrows above the line mark the boundaries of phage R37. The 2.9-kb BamHI-EcoRI restriction fragment is altered in spi dppta (data not shown). The open box below the line indicates the 1.3-kb EcoRI genomic fragment used to isolate the various spi cDNAs. This 1.3-kb fragment ...
... indicated by the top line. Arrows above the line mark the boundaries of phage R37. The 2.9-kb BamHI-EcoRI restriction fragment is altered in spi dppta (data not shown). The open box below the line indicates the 1.3-kb EcoRI genomic fragment used to isolate the various spi cDNAs. This 1.3-kb fragment ...
Type I Factor XI11 Deficiency Is Caused By a
... sequenced, all of the 15 ssDNA samples showed ATGGTTA U C A T C (Fig 3), while the normal sequence is ATGGTTACATC. The insertion of this AAC triplet falls within a codon forTyr-80: between its first and secondnucleotides, -adenosine-5285 and cytidine-5286,” resulting in the creation of a stop codon ...
... sequenced, all of the 15 ssDNA samples showed ATGGTTA U C A T C (Fig 3), while the normal sequence is ATGGTTACATC. The insertion of this AAC triplet falls within a codon forTyr-80: between its first and secondnucleotides, -adenosine-5285 and cytidine-5286,” resulting in the creation of a stop codon ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
as a PDF - CiteSeerX
... replication by the LIR, leading to recombinationally active initiating structures. Such interactions predict replication-related, MMR-independent genome changes. tions in higher organisms are proposed to interact with mismatches in some way to inhibit homeologous recombination, possibly by preventin ...
... replication by the LIR, leading to recombinationally active initiating structures. Such interactions predict replication-related, MMR-independent genome changes. tions in higher organisms are proposed to interact with mismatches in some way to inhibit homeologous recombination, possibly by preventin ...
lilliputian - Development - The Company of Biologists
... cDNA (Fig. 1D). Comparison of cDNA sequence to genomic sequence revealed a large transcription unit that spans approx. 68 kb (Fig. 1D). The gene contains 13 exons and the full-length lilli transcript is 8,516 bp in length. Conceptual translation of the open reading frame (ORF) yields a protein of 1, ...
... cDNA (Fig. 1D). Comparison of cDNA sequence to genomic sequence revealed a large transcription unit that spans approx. 68 kb (Fig. 1D). The gene contains 13 exons and the full-length lilli transcript is 8,516 bp in length. Conceptual translation of the open reading frame (ORF) yields a protein of 1, ...
NCBI Molecular Biology Resources
... COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M17755.2 and AW874082.1. On Feb 25, 2003 this sequence version replaced gi:21361188. ...
... COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M17755.2 and AW874082.1. On Feb 25, 2003 this sequence version replaced gi:21361188. ...
History of Discoveries in Molecular Biology
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
... invented a technique for multiplying DNA sequences in vitro by, the polymerase chain reaction - PCR. PCR has been called the most revolutionary new technique in molecular biology in the 1980s. Cetus patented the process, and in the summer of 1991 sold the patent to Hoffman-La Roche, Inc. for $300 mi ...
A Selective Sweep Driven by Pyrimethamine Treatment in Southeast
... years has been well documented, the full genome sequence and a microsatellite map are now available, and haplotype data can be easily generated. We examined microsatellite variation around the dihydrofolate reductase (dhfr) gene on chromosome 4 of P. falciparum. Point mutations in dhfr are known to ...
... years has been well documented, the full genome sequence and a microsatellite map are now available, and haplotype data can be easily generated. We examined microsatellite variation around the dihydrofolate reductase (dhfr) gene on chromosome 4 of P. falciparum. Point mutations in dhfr are known to ...
Computational Identification of Plant MicroRNAs and
... plant miRNAs have a strong propensity to target genes controlling development, particularly those of transcription factors and F-box proteins. However, plant miRNAs have conserved regulatory functions extending beyond development, in that they also target superoxide dismutases, laccases, and ATP sul ...
... plant miRNAs have a strong propensity to target genes controlling development, particularly those of transcription factors and F-box proteins. However, plant miRNAs have conserved regulatory functions extending beyond development, in that they also target superoxide dismutases, laccases, and ATP sul ...
View PDF - OSU Biochemistry and Molecular Biology
... required for appendage formation, encodes a homeobox transcription factor and is one of the high-level executives regulated by Hox proteins. The area of Dll expression in the embryo (shown as grey patches) corresponds to sites where in the course of development imaginal discs and, subsequently, appe ...
... required for appendage formation, encodes a homeobox transcription factor and is one of the high-level executives regulated by Hox proteins. The area of Dll expression in the embryo (shown as grey patches) corresponds to sites where in the course of development imaginal discs and, subsequently, appe ...
Genome Biology - Department of Computer Science and
... Many computational methods have also been proposed to predict TFBSs [12-19]. These methods are mostly based on the idea that the binding of a TF is mediated by the recognition of its binding motif represented as a position specific scoring matrix (PSSM). PSSMs are usually discovered as those enrich ...
... Many computational methods have also been proposed to predict TFBSs [12-19]. These methods are mostly based on the idea that the binding of a TF is mediated by the recognition of its binding motif represented as a position specific scoring matrix (PSSM). PSSMs are usually discovered as those enrich ...
Bcl-2–Modifying Factor Induces Renal Proximal Tubular
... which insulin reversed this finding. In vitro, Bmf cDNA overexpression in rat RPTCs coimmunoprecipated with Bcl-2, enhanced caspase-3 activity, and promoted apoptosis. High glucose (25 mmol/L) induced Bmf mRNA expression in RPTCs, whereas rotenone, catalase, diphenylene iodinium, and apocynin decreas ...
... which insulin reversed this finding. In vitro, Bmf cDNA overexpression in rat RPTCs coimmunoprecipated with Bcl-2, enhanced caspase-3 activity, and promoted apoptosis. High glucose (25 mmol/L) induced Bmf mRNA expression in RPTCs, whereas rotenone, catalase, diphenylene iodinium, and apocynin decreas ...
Novel mutants of 23S RNA: characterization of
... Plasmid pLST1067T, containing the rrnB operon with single point mutation A to T at the position 1067 was a kind gift from Dr J. Thompson (1). Plasmid pNO2680 contains the E.coii rrnB operon under control of the lambda leftward promoter (11) and plasmid ptBsB contains the E. coli 23S RNA structural g ...
... Plasmid pLST1067T, containing the rrnB operon with single point mutation A to T at the position 1067 was a kind gift from Dr J. Thompson (1). Plasmid pNO2680 contains the E.coii rrnB operon under control of the lambda leftward promoter (11) and plasmid ptBsB contains the E. coli 23S RNA structural g ...
Cytokinin and auxin have a crucial role in the control of this balance.
... [14,17] and induce the transcription of cytokinin primary response genes, including type-A ARRs [14,16]. Whereas type-B ARRs are positive regulators of the cytokinin response [14,17], type-A ARRs are negative regulators of cytokinin signalling [14,16] (Figure 1). Thus, as in the case of auxin signal ...
... [14,17] and induce the transcription of cytokinin primary response genes, including type-A ARRs [14,16]. Whereas type-B ARRs are positive regulators of the cytokinin response [14,17], type-A ARRs are negative regulators of cytokinin signalling [14,16] (Figure 1). Thus, as in the case of auxin signal ...
What percentage of students have a dominant learning style
... inheritance follows a bell curve? ...
... inheritance follows a bell curve? ...
BT314 Virology
... Associates/Science Source Photo Researchers; bottom, courtesy of Eric Lander, Whitehead Institute, MIT.) ...
... Associates/Science Source Photo Researchers; bottom, courtesy of Eric Lander, Whitehead Institute, MIT.) ...
Introduction to Genetics
... Associates/Science Source Photo Researchers; bottom, courtesy of Eric Lander, Whitehead Institute, MIT.) ...
... Associates/Science Source Photo Researchers; bottom, courtesy of Eric Lander, Whitehead Institute, MIT.) ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.