Selection for TnlO Tet Repressor Binding to tet Operator
... the absence is indicated br ''-- (pWH1200). The final concentrations of inducers were 10- M for IPTG and0.5 pg/ml (corresponding to subinhibitory amounts) for tetracycline. ...
... the absence is indicated br ''-- (pWH1200). The final concentrations of inducers were 10- M for IPTG and0.5 pg/ml (corresponding to subinhibitory amounts) for tetracycline. ...
Identification and isolation of active N2O reducers in rice paddy soil
... was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugation. Denaturing gradient gel electrophoresis and clone library analysis targeting the 16S ...
... was used to identify succinate-assimilating microbes under N2O-reducing conditions. DNA was extracted 24 h after incubation, and heavy and light DNA fractions were separated by density gradient ultracentrifugation. Denaturing gradient gel electrophoresis and clone library analysis targeting the 16S ...
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and
... activity by FRQ via negative feedback is indispensable for clock function. An unbiased genetic screen that targeted mutants with defects in negative feedback regulation yielded a fully viable arrhythmic strain bearing a novel allele of FRQ-interacting RNA helicase (frh), an essential gene which enco ...
... activity by FRQ via negative feedback is indispensable for clock function. An unbiased genetic screen that targeted mutants with defects in negative feedback regulation yielded a fully viable arrhythmic strain bearing a novel allele of FRQ-interacting RNA helicase (frh), an essential gene which enco ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... knockdown and exogenous overexpression of GFP-tagged RIF1 cDNA and mutant GFP-tagged RIF1 cDNA allows the authors to gain a transient look at RIF1 interactions in a human cell line. The authors show that the exogenously expressed GFP-RIF1 cDNA in their system does interact with all three isoforms of ...
... knockdown and exogenous overexpression of GFP-tagged RIF1 cDNA and mutant GFP-tagged RIF1 cDNA allows the authors to gain a transient look at RIF1 interactions in a human cell line. The authors show that the exogenously expressed GFP-RIF1 cDNA in their system does interact with all three isoforms of ...
0820_Lee_GF3A3 - Amazon Web Services
... Primary Lymphedema Not all gene mutations will result in a phenotype that reflects a major impact on lymphatic function. A minor impact among patients with primary lymphedema has been also reported for HGF and MET genes. Due to incomplete penetrance, some individuals of the same family can remain a ...
... Primary Lymphedema Not all gene mutations will result in a phenotype that reflects a major impact on lymphatic function. A minor impact among patients with primary lymphedema has been also reported for HGF and MET genes. Due to incomplete penetrance, some individuals of the same family can remain a ...
Anterior boundaries of Hox gene expression in mesoderm
... els of Hox 1.1 transcripts are detected in RNAs from embryos at 12 days post coitum (P.c.) and multiple transcripts are found in adult testis, kidney, brain, and ovary [q.Restricted transcription of the Hox 1.3 gene to thoracic sclerotomes, as well as tissues derived from or induced by thoracic meso ...
... els of Hox 1.1 transcripts are detected in RNAs from embryos at 12 days post coitum (P.c.) and multiple transcripts are found in adult testis, kidney, brain, and ovary [q.Restricted transcription of the Hox 1.3 gene to thoracic sclerotomes, as well as tissues derived from or induced by thoracic meso ...
Genetics Principles And Analysis
... ABOUT THE AUTHORS Daniel L. Hartl is a Professor of Biology at Harvard University. He received his B.S. degree and Ph.D. from the University of Wisconsin. His research interests include molecular genetics, molecular evolution, and population genetics. Elizabeth W. Jones is a Professor of Biological ...
... ABOUT THE AUTHORS Daniel L. Hartl is a Professor of Biology at Harvard University. He received his B.S. degree and Ph.D. from the University of Wisconsin. His research interests include molecular genetics, molecular evolution, and population genetics. Elizabeth W. Jones is a Professor of Biological ...
Simple identification of dominant p53 mutants by
... mutant cells should contain a mixture of wild-type, mixed and mutant tetramers in the ratio 1:14:1, provided the mutant protein is able to form oligomers with the wild-type. The extent to which p53 mutants really do abrogate wild-type p53 function in the heterozygous state is controversial and depen ...
... mutant cells should contain a mixture of wild-type, mixed and mutant tetramers in the ratio 1:14:1, provided the mutant protein is able to form oligomers with the wild-type. The extent to which p53 mutants really do abrogate wild-type p53 function in the heterozygous state is controversial and depen ...
Rotaphor System 6.0
... In contrast to conventional PFGE instruments with fixed electrodes the patented Rotaphor® system features free rotating electrodes. Thus any angle can be set for the electric field. Changing field angles over the time are achieved by the precise movement of the rotor. This feature is of special impo ...
... In contrast to conventional PFGE instruments with fixed electrodes the patented Rotaphor® system features free rotating electrodes. Thus any angle can be set for the electric field. Changing field angles over the time are achieved by the precise movement of the rotor. This feature is of special impo ...
jing is required for wing development and to establish the
... in the Drosophila leg have additional and very distinct functions at other stages and tissues during development. For example, dac plays an important role in the development of the eye, central nervous system (CNS), and trachea (MARDON et al. 1994; MARTINI et al. 2000; SHEN and MARDON 1997). In addi ...
... in the Drosophila leg have additional and very distinct functions at other stages and tissues during development. For example, dac plays an important role in the development of the eye, central nervous system (CNS), and trachea (MARDON et al. 1994; MARTINI et al. 2000; SHEN and MARDON 1997). In addi ...
Molecular and Genetic Characterization of a
... changes in color, flavor, and texture. Ripening is a tightly controlled and highly programmed developmental event. Identifying the components of this developmental switch is important not only for manipulating this key plant process but also for understanding the regulation of plant development. The ...
... changes in color, flavor, and texture. Ripening is a tightly controlled and highly programmed developmental event. Identifying the components of this developmental switch is important not only for manipulating this key plant process but also for understanding the regulation of plant development. The ...
4 configuration file
... annotation flag 'CSN' (e.g. CSN=c.421A>G_p.Thr141Ala). For indels, CSN is created based on the correctly aligned (strand-aware) representation of the variant. Note that the CSN annotation is given both on DNA (c.) and protein (p.) level if appropriate (otherwise only c. is given). The CLASS and SO f ...
... annotation flag 'CSN' (e.g. CSN=c.421A>G_p.Thr141Ala). For indels, CSN is created based on the correctly aligned (strand-aware) representation of the variant. Note that the CSN annotation is given both on DNA (c.) and protein (p.) level if appropriate (otherwise only c. is given). The CLASS and SO f ...
CIRCADIAN RHYTHMS IN PLANTS C Robertson McClung
... stage under the specific growth conditions sampled, and it will take many iterations to exhaustively sample all possible developmental stages and environmental conditions. Nor will these initial experiments identify genes whose induction or repression in response to environmental or biological stimu ...
... stage under the specific growth conditions sampled, and it will take many iterations to exhaustively sample all possible developmental stages and environmental conditions. Nor will these initial experiments identify genes whose induction or repression in response to environmental or biological stimu ...
ACTIN-RELATED PROTEIN6 Regulates Female Meiosis By
... The SWR1 complex is a likely candidate to regulate gene expression during meiosis, and its role in this process is just beginning to be understood. Meta-analysis of genome-wide H2A.Z occupancy in Schizosaccharomyces pombe using the Podbat computational tool showed preferential H2A.Z incorporation in ...
... The SWR1 complex is a likely candidate to regulate gene expression during meiosis, and its role in this process is just beginning to be understood. Meta-analysis of genome-wide H2A.Z occupancy in Schizosaccharomyces pombe using the Podbat computational tool showed preferential H2A.Z incorporation in ...
Bridging the transgenerational gap with epigenetic memory
... [31]. Genetically wild type descendants from a cross between wild type males and hermaphrodites with mutations in the wdr-5 or set-2 genes were long lived for up to three generations compared with descendants from pure wild type ancestors [27]. The long lifespan of the descendants is dependent on th ...
... [31]. Genetically wild type descendants from a cross between wild type males and hermaphrodites with mutations in the wdr-5 or set-2 genes were long lived for up to three generations compared with descendants from pure wild type ancestors [27]. The long lifespan of the descendants is dependent on th ...
Chromosomes Carrying Meiotic Avoidance Loci
... praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspores, forming unreduced aposporous embryo sacs, and the sexual program ceases. LOA-linked genetic ...
... praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspores, forming unreduced aposporous embryo sacs, and the sexual program ceases. LOA-linked genetic ...
Transvection Is Common Throughout the Drosophila
... integration of transgenes is becoming increasingly common, as it provides the opportunity to control for and minimize genomic position effects (Markstein et al. 2008; Pfeiffer et al. 2010). Given the use of multiple transgenes in many crossing schemes, it is common to use two transgenes that have be ...
... integration of transgenes is becoming increasingly common, as it provides the opportunity to control for and minimize genomic position effects (Markstein et al. 2008; Pfeiffer et al. 2010). Given the use of multiple transgenes in many crossing schemes, it is common to use two transgenes that have be ...
a complex voyage to the X chromosome
... an RNA/DNA helicase with multiple RNA-interaction domains, including its N-terminal double-stranded RNA-binding (RB) motifs and its C-terminal glycine-rich domain (Lee et al., 1997; Izzo et al., 2008). (F,G) The roX box elements (red) and inverse roX boxes (blue) are proposed to mediate the formatio ...
... an RNA/DNA helicase with multiple RNA-interaction domains, including its N-terminal double-stranded RNA-binding (RB) motifs and its C-terminal glycine-rich domain (Lee et al., 1997; Izzo et al., 2008). (F,G) The roX box elements (red) and inverse roX boxes (blue) are proposed to mediate the formatio ...
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... Nishida, 1997; Stolfi et al., 2010) by observing formation of the BWM by time-lapse imaging. We found that, indeed, the BWM formed as an extension of the ASM (supplementary material Movie 1). Thus, the absence of the BWM in Ci-Hox1-deficient juveniles is likely to be due to disruption of ASM formati ...
... Nishida, 1997; Stolfi et al., 2010) by observing formation of the BWM by time-lapse imaging. We found that, indeed, the BWM formed as an extension of the ASM (supplementary material Movie 1). Thus, the absence of the BWM in Ci-Hox1-deficient juveniles is likely to be due to disruption of ASM formati ...
Properties of Mitotic and Meiotic Recombination in the
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
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... larvae, whose polymorphic epidermal cells sequentially produce first larval, then pupal and finally adult structures (Riddiford, 1994), while internally growing imaginal discs, if any occur, give rise to appendages only, particularly the wings (Svacha, 1992). Seminal studies in Drosophila have defin ...
... larvae, whose polymorphic epidermal cells sequentially produce first larval, then pupal and finally adult structures (Riddiford, 1994), while internally growing imaginal discs, if any occur, give rise to appendages only, particularly the wings (Svacha, 1992). Seminal studies in Drosophila have defin ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
Trans-acting siRNA-mediated repression of ETTIN
... The interaction between zip-2 and ett-7, ett-15 and arf4-2 was studied in F2 families generated by the self-pollination of plants heterozygous for both mutations. The genotype of the plants in these families was determined by PCR, using allele-specific primers. The first two leaves of double mutants ...
... The interaction between zip-2 and ett-7, ett-15 and arf4-2 was studied in F2 families generated by the self-pollination of plants heterozygous for both mutations. The genotype of the plants in these families was determined by PCR, using allele-specific primers. The first two leaves of double mutants ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.