Development of a repressible mycobacterial
... these tools proved to be quite useful, they have the disadvantage that Tc functions as an inducer of gene expression and has therefore to be removed to silence the gene under investigation. For this reason Guo et al. (13) developed a modified system using a mutated TetR able to bind tetO only in the ...
... these tools proved to be quite useful, they have the disadvantage that Tc functions as an inducer of gene expression and has therefore to be removed to silence the gene under investigation. For this reason Guo et al. (13) developed a modified system using a mutated TetR able to bind tetO only in the ...
Standards and guidelines for the interpretation of sequence
... A mutation is defined as a permanent change in the nucleotide sequence, whereas a polymorphism is defined as a variant with a frequency above 1%. The terms “mutation” and “polymorphism,” however, which have been used widely, ...
... A mutation is defined as a permanent change in the nucleotide sequence, whereas a polymorphism is defined as a variant with a frequency above 1%. The terms “mutation” and “polymorphism,” however, which have been used widely, ...
Molecular characterization of glutathione peroxidase
... tribution in mammalian species showed that GPx1 is ubiquitous, whereas GPx2 is mainly restricted to the gastrointestinal tract and human liver (but not rat liver) (8, 28). However, in the present study, silver carp GPx was widely expressed in all major tissues examined including liver, adipose tissu ...
... tribution in mammalian species showed that GPx1 is ubiquitous, whereas GPx2 is mainly restricted to the gastrointestinal tract and human liver (but not rat liver) (8, 28). However, in the present study, silver carp GPx was widely expressed in all major tissues examined including liver, adipose tissu ...
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
Annotation Strategy Guide - GEP Community Server
... Found on contig37, the blastx track on the Genome Browser reveals an alignment that only covers a small part of the first exon. Consequently, we will need to do a more sensitive tblastn search to determine if there is any additional region of conservation to the first exon. The second exon of Dyrk3 ...
... Found on contig37, the blastx track on the Genome Browser reveals an alignment that only covers a small part of the first exon. Consequently, we will need to do a more sensitive tblastn search to determine if there is any additional region of conservation to the first exon. The second exon of Dyrk3 ...
Programmed Ribosomal Frameshifting Generates a Copper
... pattern of distribution of ribosome progression along the gene suggested that expression of copA might be a subject of idiosyncratic regulation. One of the mechanisms exploited by cells for expanding the spectrum of proteins expressed from a limited number of genomic open reading frames (ORFs) is tr ...
... pattern of distribution of ribosome progression along the gene suggested that expression of copA might be a subject of idiosyncratic regulation. One of the mechanisms exploited by cells for expanding the spectrum of proteins expressed from a limited number of genomic open reading frames (ORFs) is tr ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
... pollen due to cross-reactivity between the major birch pollen allergen, Bet v 1, and the apple allergen Mal d 1 (Fritsch et al. 1998). The cross-reactivity of these allergens is due to their high amino acid sequence and structural similarity (Vanek-Krebitz et al. 1995). Bet v 1-like genes have also ...
... pollen due to cross-reactivity between the major birch pollen allergen, Bet v 1, and the apple allergen Mal d 1 (Fritsch et al. 1998). The cross-reactivity of these allergens is due to their high amino acid sequence and structural similarity (Vanek-Krebitz et al. 1995). Bet v 1-like genes have also ...
Signaling Pathways of Heme Oxygenase
... The toxic effects of CO have been known for many years. Since CO binds hemoglobin with higher affinity than O2, O2 delivery to tissues and organs is blocked by this gaseous molecule. More recently, HO-derived CO has been recognized to be an important cellular messenger with various physiological fun ...
... The toxic effects of CO have been known for many years. Since CO binds hemoglobin with higher affinity than O2, O2 delivery to tissues and organs is blocked by this gaseous molecule. More recently, HO-derived CO has been recognized to be an important cellular messenger with various physiological fun ...
Inhibition of Wound-Induced Accumulation of
... et al., 1993) and components of the JA biosynthetic pathway (Farmer and Ryan, 1992; Peña-Cortés et al., 1993, 1995) initiates the accumulation of several mRNAs from genes involved in different physiological plant processes (Sembdner and Parthier, 1993; Farmer, 1994). For instance, JA spraying of t ...
... et al., 1993) and components of the JA biosynthetic pathway (Farmer and Ryan, 1992; Peña-Cortés et al., 1993, 1995) initiates the accumulation of several mRNAs from genes involved in different physiological plant processes (Sembdner and Parthier, 1993; Farmer, 1994). For instance, JA spraying of t ...
The DUET gene is necessary for chromosome
... pollen development by examining cleared anthers as well as plastic sections. Early stages of pollen development corresponding to anther stage 5 (Sanders et al., 1999) were normal (Fig. 2). The endothecium and internal layers surrounding the microsporocyte were indistinguishable from wild type as was ...
... pollen development by examining cleared anthers as well as plastic sections. Early stages of pollen development corresponding to anther stage 5 (Sanders et al., 1999) were normal (Fig. 2). The endothecium and internal layers surrounding the microsporocyte were indistinguishable from wild type as was ...
Characterization of Five Brevibacillus Bacteriophages and Their
... withstand harsh treatments used to isolate them. Most hives are considered to be latent carriers of the endospores and any effective eradication of the pathogen must address the presence of endospores (Genersch, 2010). Hives infected with Foulbrood Disease are commonly burned in order to prevent it ...
... withstand harsh treatments used to isolate them. Most hives are considered to be latent carriers of the endospores and any effective eradication of the pathogen must address the presence of endospores (Genersch, 2010). Hives infected with Foulbrood Disease are commonly burned in order to prevent it ...
Molecular cloning, characterization and gene expression of an
... changed in Penaeus monodon after WSSV infection. However, the genetic information about catalase in freshwater giant prawn Macrobrachium rosenbergii is very limited. In our earlier findings, we reported [45,46] that freshwater giant prawn M. rosenbergii industry is affected all over the World due to ...
... changed in Penaeus monodon after WSSV infection. However, the genetic information about catalase in freshwater giant prawn Macrobrachium rosenbergii is very limited. In our earlier findings, we reported [45,46] that freshwater giant prawn M. rosenbergii industry is affected all over the World due to ...
Agrobacterium: nature`s genetic engineer
... Since the biological functions encoded by these genes were not yet known, the full significance of these data could not be appreciated. However, the use of promoters encoded in the T-DNA proved invaluable in the genetic engineering of plants. Once a restriction map of an octopine Ti plasmid was gene ...
... Since the biological functions encoded by these genes were not yet known, the full significance of these data could not be appreciated. However, the use of promoters encoded in the T-DNA proved invaluable in the genetic engineering of plants. Once a restriction map of an octopine Ti plasmid was gene ...
Probable presence of an ubiquitous cryptic mitochondrial gene on
... gene has been named gau for gene antisense ubiquitous in mtDNAs. The length of the deduced protein is approximately 100 amino acids. In vertebrates, several stop codons have been found in the mt gau region, and potentially functional gau regions have been found in nuclear genomes. However, a recent ...
... gene has been named gau for gene antisense ubiquitous in mtDNAs. The length of the deduced protein is approximately 100 amino acids. In vertebrates, several stop codons have been found in the mt gau region, and potentially functional gau regions have been found in nuclear genomes. However, a recent ...
Homeotic genes regulate the spatial expression
... part of each parasegment (PS; Baker, 1987) and is one of several Drosophila genes involved in establishing polarity of the embryo (Ingham, 1988). The protein is made in about one-quarter of the cells in each segment primordium, where the RNA is seen, and then some of the protein appears to move into ...
... part of each parasegment (PS; Baker, 1987) and is one of several Drosophila genes involved in establishing polarity of the embryo (Ingham, 1988). The protein is made in about one-quarter of the cells in each segment primordium, where the RNA is seen, and then some of the protein appears to move into ...
The daily rhythm of mice
... particular finding suggested that peripheral oscillators are directly involved in the control of rhythmic physiological processes (see below). In Mop3/Bmal1 knock out mice, the expression of the related Bmal2 gene was drastically down regulated [17,21]. A transgenic rescue of Bmal2 expression in Mop3 ...
... particular finding suggested that peripheral oscillators are directly involved in the control of rhythmic physiological processes (see below). In Mop3/Bmal1 knock out mice, the expression of the related Bmal2 gene was drastically down regulated [17,21]. A transgenic rescue of Bmal2 expression in Mop3 ...
Wolbachia John H. Werren and Jeremy D. Bartos
... indels (in indels, a hyphen [-] indicates one base pair), the Protocalliphora sequence has the following spatial pattern (5⬘→3⬘): 17Nv 34Ng - 1Nv - 1Ng. The chance probability of a string of 34 Ng similarities in 53 shared polymorphisms is remarkably low (p ⬍ 10⫺10), and this indicates recombination ...
... indels (in indels, a hyphen [-] indicates one base pair), the Protocalliphora sequence has the following spatial pattern (5⬘→3⬘): 17Nv 34Ng - 1Nv - 1Ng. The chance probability of a string of 34 Ng similarities in 53 shared polymorphisms is remarkably low (p ⬍ 10⫺10), and this indicates recombination ...
A TaqI polymorphism in the 3 UTR of the IL-12
... The fact that the TaqI polymorphism in the p40 gene has an effect on IL-12 p70 secretion, but not on IL-12 p40 secretion, is of considerable interest and, at first glance, may appear counterintuitive. One possibility is that the polymorphism affects p40 homodimer formation, which, in turn, influence ...
... The fact that the TaqI polymorphism in the p40 gene has an effect on IL-12 p70 secretion, but not on IL-12 p40 secretion, is of considerable interest and, at first glance, may appear counterintuitive. One possibility is that the polymorphism affects p40 homodimer formation, which, in turn, influence ...
Risk assessment - Office of the Gene Technology Regulator
... might give rise to harm to people or the environment. These 16 events included consideration of whether, or not, expression of the introduced genes could result in products that are toxic or allergenic to people or other organisms, alter characteristics that may impact on the spread and persistence ...
... might give rise to harm to people or the environment. These 16 events included consideration of whether, or not, expression of the introduced genes could result in products that are toxic or allergenic to people or other organisms, alter characteristics that may impact on the spread and persistence ...
This document has been downloaded from Tampub – The
... where it can be processed by two specific proteases and then release the amino-terminal transcription-activation domain of the SREBF. The active form of SREBF can then travel to the nucleus where it binds to the promoters of target genes [1]. The SREBFs consist of three different SREBF isoforms, SRE ...
... where it can be processed by two specific proteases and then release the amino-terminal transcription-activation domain of the SREBF. The active form of SREBF can then travel to the nucleus where it binds to the promoters of target genes [1]. The SREBFs consist of three different SREBF isoforms, SRE ...
DYAD in meiotic chromosome organisation - Development
... CAPS markers, primers were designed to PCR amplify a 1-2 kb region of genomic DNA which was then digested with a panel of restriction enzymes, and electrophoresed on a gel to identify polymorphisms between the Ler and No-O ecotypes and in some cases between Ler and Col-O. Recombinants north and sout ...
... CAPS markers, primers were designed to PCR amplify a 1-2 kb region of genomic DNA which was then digested with a panel of restriction enzymes, and electrophoresed on a gel to identify polymorphisms between the Ler and No-O ecotypes and in some cases between Ler and Col-O. Recombinants north and sout ...
Anterior boundaries of Hox gene expression in mesoderm
... gene may encode positional information along the rostralcaudal axis [9]. Transcripts of the Hox 1.5 gene are expressed in mesoderm and ectoderm of 7- to 9-day p.c. embryos, but are spatially restricted to the ectoderm-derived neural tube, myencephalon, dorsal root ganglia and many mesodermderived ti ...
... gene may encode positional information along the rostralcaudal axis [9]. Transcripts of the Hox 1.5 gene are expressed in mesoderm and ectoderm of 7- to 9-day p.c. embryos, but are spatially restricted to the ectoderm-derived neural tube, myencephalon, dorsal root ganglia and many mesodermderived ti ...
PCB Biodegradation and bphA1 Gene Expression Induced by
... Two PCB-degrading bacteria were chosen for investigation in this study, one was a new isolate (Pseudomonas fluorescence P2W) from a top red earth soil in southern China and the other (Ralstonia eutropha H850) was from a bacterial collection and reportedly isolated from PCB-contaminated sediment envi ...
... Two PCB-degrading bacteria were chosen for investigation in this study, one was a new isolate (Pseudomonas fluorescence P2W) from a top red earth soil in southern China and the other (Ralstonia eutropha H850) was from a bacterial collection and reportedly isolated from PCB-contaminated sediment envi ...