Questions Ch 24
... Chapter 24--control of microbial growth 3) Ampicillin is a semi-synthetic antibiotic derivative of penicillin G. Ampicillin is acidresistant, whereas penicillin G is not resistant to acid. Why is this important? a) Ampicillin is resistant to β-lactamases. b) Ampicillin can be taken orally. c) Ampici ...
... Chapter 24--control of microbial growth 3) Ampicillin is a semi-synthetic antibiotic derivative of penicillin G. Ampicillin is acidresistant, whereas penicillin G is not resistant to acid. Why is this important? a) Ampicillin is resistant to β-lactamases. b) Ampicillin can be taken orally. c) Ampici ...
Mentor: James A. MacKay Students: Amanda Williams, Holly Sofka
... Students: Amanda Williams, Holly Sofka Project Description: Ribonucleic acid (RNA) is believed to be an important molecule in the evolution of life and has functionally taken on many important biological roles. Given the many functions of RNA, molecular recognition of RNA represents an attractive go ...
... Students: Amanda Williams, Holly Sofka Project Description: Ribonucleic acid (RNA) is believed to be an important molecule in the evolution of life and has functionally taken on many important biological roles. Given the many functions of RNA, molecular recognition of RNA represents an attractive go ...
Chapter Twenty-Seven: Amino Acids
... o Join together (with DCC as activator) b. Merrifield Solid Phase synthesis (28.8) o Protect N-terminus o Bond carboxylate of aa2 to solid support (via SN2 with Benzyl chloride branches) o Remove N-protection w dilute base o Rinse solid support o Add second N-protected amino acid via C-terminus ...
... o Join together (with DCC as activator) b. Merrifield Solid Phase synthesis (28.8) o Protect N-terminus o Bond carboxylate of aa2 to solid support (via SN2 with Benzyl chloride branches) o Remove N-protection w dilute base o Rinse solid support o Add second N-protected amino acid via C-terminus ...
Antibiotics - Dr Magrann
... NAM SYNTHESIS: D-cycloserine mimics D-Alanine, prevents synthesis. SHUTTLE: Bacitracin interferes with C55 lipid shuttle by binding it. TRANSGLYCOSYLATION: Glycopeptides (e.g. Vancomycin) prevents it. CROSSLINKAGE: b- lactams mimic D-ALA-D-ALA of NAM and interfere with the enzymes that do the crossl ...
... NAM SYNTHESIS: D-cycloserine mimics D-Alanine, prevents synthesis. SHUTTLE: Bacitracin interferes with C55 lipid shuttle by binding it. TRANSGLYCOSYLATION: Glycopeptides (e.g. Vancomycin) prevents it. CROSSLINKAGE: b- lactams mimic D-ALA-D-ALA of NAM and interfere with the enzymes that do the crossl ...
So, you want to know about siderophore synthesis
... These derivatives allowed synthesis of several siderophores and their analogues ...
... These derivatives allowed synthesis of several siderophores and their analogues ...
From boron analogues of amino acids to boronated DNA
... degrees. Some of the important modifications which have been incorporated into oligonucleotide backbone are phosphotriesters (-POR), methylphosphonates(-PCH3), phosphorothioates(-P=S), phosphorodithioates (S=PS) and phosphoramidates (-PNR2). These analogs have been shown to inhibit various viruses e ...
... degrees. Some of the important modifications which have been incorporated into oligonucleotide backbone are phosphotriesters (-POR), methylphosphonates(-PCH3), phosphorothioates(-P=S), phosphorodithioates (S=PS) and phosphoramidates (-PNR2). These analogs have been shown to inhibit various viruses e ...
The Synthesis and Expression of Peptide CbnY Thomas Doerksen
... recently isolated and reported, but initial structural studies and sequence homology suggests that it is part of a two-component bacteriocin. To test this hypothesis, the proposed second component CbnY, a 33 amino acid peptide predicted by genetics, was prepared by both solid phase peptide synthesis ...
... recently isolated and reported, but initial structural studies and sequence homology suggests that it is part of a two-component bacteriocin. To test this hypothesis, the proposed second component CbnY, a 33 amino acid peptide predicted by genetics, was prepared by both solid phase peptide synthesis ...
CHAP Twenty-Five - Foothill College
... D) Separation and Identification of aa fragments via Gel electrophoresis, Ionexchange chromatography, and Mass Spectrometry 4) Peptide Synthesis A) Traditional solution synthesis i) Role of DCC ii) N-protecting groups: BOC and FMOC iii) C-protecting groups: PhCH2OH and 1° ROH B) Merrifield Solid Pha ...
... D) Separation and Identification of aa fragments via Gel electrophoresis, Ionexchange chromatography, and Mass Spectrometry 4) Peptide Synthesis A) Traditional solution synthesis i) Role of DCC ii) N-protecting groups: BOC and FMOC iii) C-protecting groups: PhCH2OH and 1° ROH B) Merrifield Solid Pha ...
Microsoft Word
... One of the major current challenges before chemists to develop synthetic methods that are less polluting i.e. to design clean or green chemicals transformations. The chemical manufacturing processes should be such that they don’t cause permanent damage to the environment or disturb the ecological ba ...
... One of the major current challenges before chemists to develop synthetic methods that are less polluting i.e. to design clean or green chemicals transformations. The chemical manufacturing processes should be such that they don’t cause permanent damage to the environment or disturb the ecological ba ...
Nonstandard Hydrogen Bonding in Duplex Oligonucleotides. The
... for this epimerization (a! to ,8) was 0.070 min-l (flp(Rcud0) = 10 min; 0.1 M Et3NH+-OAc, pH 7.1, containing 10%MeCN). The same equilibrium ratio of products was obtained starting from oligonucleotides containing either anomer. While epimerization does not preclude laboratory experiments such as tho ...
... for this epimerization (a! to ,8) was 0.070 min-l (flp(Rcud0) = 10 min; 0.1 M Et3NH+-OAc, pH 7.1, containing 10%MeCN). The same equilibrium ratio of products was obtained starting from oligonucleotides containing either anomer. While epimerization does not preclude laboratory experiments such as tho ...
Biosynthesis of monomers
... • Glucose synthesis from organic compounds – Process is called gluconeogenesis – Most cells can carry out gluconeogenesis from phosphoenolpyruvate – Many bacteria can use oxaloacetate as starting material ...
... • Glucose synthesis from organic compounds – Process is called gluconeogenesis – Most cells can carry out gluconeogenesis from phosphoenolpyruvate – Many bacteria can use oxaloacetate as starting material ...
Assn5
... 5. Describe a biochemical procedure that selectively inhibits DNA synthesis, but has no affect on RNA synthesis. Answer this question by identifying a compound that inhibits one of the enzymes you showed in question 4. 5 Point Bonus 1. Beginning with NaNO2, trace the path of nitrogen into the struct ...
... 5. Describe a biochemical procedure that selectively inhibits DNA synthesis, but has no affect on RNA synthesis. Answer this question by identifying a compound that inhibits one of the enzymes you showed in question 4. 5 Point Bonus 1. Beginning with NaNO2, trace the path of nitrogen into the struct ...
Nucleic Acids - faculty at Chemeketa
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
Oligonucleotide synthesis
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not suffer from this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks derived from protected 2'-deoxynucleosides (dA, dC, dG, and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, e.g. LNA, BNA.To obtain the desired oligonucleotide, the building blocks are sequentially coupled to the growing oligonucleotide chain in the order required by the sequence of the product (see Synthetic cycle below). The process has been fully automated since the late 1970s. Upon the completion of the chain assembly, the product is released from the solid phase to solution, deprotected, and collected. The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15–25 bases in length.Oligonucleotides find a variety of applications in molecular biology and medicine. They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes.