Partial Purifiaction of β-1,3-glucan synthases and
... By submitting this thesis electronically, I declare that the entirety of the work contained therein is my own, original work, and that I have not previously in its entirety or in part submitted it for obtaining any qualification. ...
... By submitting this thesis electronically, I declare that the entirety of the work contained therein is my own, original work, and that I have not previously in its entirety or in part submitted it for obtaining any qualification. ...
Nucleotide sequence of the Tn10 encoded tetracycline resistance
... Figure 7 displays the distribution of amino acids grouped according to their polarity in hydrophobic, uncharged polar, acidic, and basic residues. The dominance of hydrophobic amino acids, which make up for 58% of the total, is clearly demonstrated in this figure. With two exceptions they are evenly ...
... Figure 7 displays the distribution of amino acids grouped according to their polarity in hydrophobic, uncharged polar, acidic, and basic residues. The dominance of hydrophobic amino acids, which make up for 58% of the total, is clearly demonstrated in this figure. With two exceptions they are evenly ...
Soy Allergy Doc - Amherst College
... protein (HVP) in sauces. Multi-grain breads, doughnuts, doughnut mix and pancake mix commonly contain soy flour. Nearly all bread products available in the US now contain soy. Soy can now be found in nearly all types of foods such as meat, ice cream, cheese and french fries. Many foods are contamina ...
... protein (HVP) in sauces. Multi-grain breads, doughnuts, doughnut mix and pancake mix commonly contain soy flour. Nearly all bread products available in the US now contain soy. Soy can now be found in nearly all types of foods such as meat, ice cream, cheese and french fries. Many foods are contamina ...
Myr 8, A Novel Unconventional Myosin Expressed during Brain
... class XVI. The head domain contains a large N-terminal extension composed of multiple ankyrin repeats, which are implicated in mediating an association with the protein phosphatase 1 (PP1) catalytic subunits 1␣ and 1␥. The motor domain is ...
... class XVI. The head domain contains a large N-terminal extension composed of multiple ankyrin repeats, which are implicated in mediating an association with the protein phosphatase 1 (PP1) catalytic subunits 1␣ and 1␥. The motor domain is ...
Utilisation of Whey
... attachments (hence the "glyco-" term). Its molecular weight, based on its amino acid content, is about 7,000; however, the carbohydrate units make it behave as if it were somewhat larger. This is important because the ultrafiltration membranes used to prepare whey protein concentrates and whey prote ...
... attachments (hence the "glyco-" term). Its molecular weight, based on its amino acid content, is about 7,000; however, the carbohydrate units make it behave as if it were somewhat larger. This is important because the ultrafiltration membranes used to prepare whey protein concentrates and whey prote ...
EVOLUTIONARILY RELATED INSERTION PATHWAYS OF
... For example, the organelles contain genomes that encode some of their proteins, using their own protein synthesizing machinery. However, the protein conducting capacity of mitochondria and chloroplasts is strongly reduced because the genes originally encoded within these organelles were transferred ...
... For example, the organelles contain genomes that encode some of their proteins, using their own protein synthesizing machinery. However, the protein conducting capacity of mitochondria and chloroplasts is strongly reduced because the genes originally encoded within these organelles were transferred ...
5 The structure and function of membrane proteins
... powerful, desktop computers with performance equivalent to the previous generation of supercomputers. Many experimental techniques relied on the codevelopment of computer hardware but software has also played a vital role in protein biochemistry. We can now search databases comparing proteins at the ...
... powerful, desktop computers with performance equivalent to the previous generation of supercomputers. Many experimental techniques relied on the codevelopment of computer hardware but software has also played a vital role in protein biochemistry. We can now search databases comparing proteins at the ...
Early events in protein folding
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
... polypeptide chain searches out its final native conformation from an inconceivably large number of available conformations. A polypeptide chain of 101 amino acid residues would have to sample 3100 = 5 × 1047 conformations, if each bond connecting two consecutive residues has only three possible conf ...
Determination of the pKa of the N-terminal amino group of
... and results in the attachment of the C-terminal carboxylate of ubiquitin to a lysine side chain in the protein substrate. Chain extension occurs via addition of subsequent ubiquitin molecules to either one of the seven lysine residues of ubiquitin, or via its N-terminal α-amino group to build linear ...
... and results in the attachment of the C-terminal carboxylate of ubiquitin to a lysine side chain in the protein substrate. Chain extension occurs via addition of subsequent ubiquitin molecules to either one of the seven lysine residues of ubiquitin, or via its N-terminal α-amino group to build linear ...
Glucansucrases: mechanism of action and structure–function
... glucansucrase encoding genes in addition to structure^function relationship studies have allowed the identification of important amino acid residues and have shown that glucansucrases are composed of two functional domains: a core region (ca. 1000 amino acids) involved in sucrose binding and splitti ...
... glucansucrase encoding genes in addition to structure^function relationship studies have allowed the identification of important amino acid residues and have shown that glucansucrases are composed of two functional domains: a core region (ca. 1000 amino acids) involved in sucrose binding and splitti ...
Scientific Opinion on Dietary Reference Values for protein
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
Keyhole Limpet Hemocyanin ( KLH )
... flexible tubules of varying length). Unlike other gastropod hemocyanins, this protein does not dissociate simply by removing divalent cations from a hemocyanin suspension, although divalent cations aid in the formation of larger aggregates. With moderate changes in pH, or ionic strenght, it will rev ...
... flexible tubules of varying length). Unlike other gastropod hemocyanins, this protein does not dissociate simply by removing divalent cations from a hemocyanin suspension, although divalent cations aid in the formation of larger aggregates. With moderate changes in pH, or ionic strenght, it will rev ...
Fulltext - Jultika
... argininosuccinate lyase from Escherichia coli (eASL) have been studied using X-ray crystallography. The main focus of this study has been understanding the structure-function relationship of MCR. The eASL has been crystallized from a highly concentrated sample of purified recombinant α-methylacylCoA ...
... argininosuccinate lyase from Escherichia coli (eASL) have been studied using X-ray crystallography. The main focus of this study has been understanding the structure-function relationship of MCR. The eASL has been crystallized from a highly concentrated sample of purified recombinant α-methylacylCoA ...
The Structure of the PanD/PanZ Protein Complex
... (Lys119-Ala126, shown as sticks, and 2F0-Fc electron density maps contoured at 1 root-mean-square deviation [rmsd]), is ordered and sandwiched between the PanZ Leu66-Gly76 and Arg43-Leu58 loops. (C) PanZ-Asn45 has been identified as being critical for activation (Nozaki et al., 2012). This residue f ...
... (Lys119-Ala126, shown as sticks, and 2F0-Fc electron density maps contoured at 1 root-mean-square deviation [rmsd]), is ordered and sandwiched between the PanZ Leu66-Gly76 and Arg43-Leu58 loops. (C) PanZ-Asn45 has been identified as being critical for activation (Nozaki et al., 2012). This residue f ...
Biochemical, biophysical and interaction studies of the stress
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
Novel Allergen Structures with Tandem Amino Acid Repeats
... Inc.). Protein sequences were compared with the GenBank, EBI, DDBJ, PDB, SwissProt, SPupdate, PIR data bases using FASTA and BLAST (18, 19). Sequence alignments were made with Clustal W (accessible via Internet site: http://www2.ebi.ac.uk/clustalw/) and GCG (Framealign, Gap) (20). The GCG package wa ...
... Inc.). Protein sequences were compared with the GenBank, EBI, DDBJ, PDB, SwissProt, SPupdate, PIR data bases using FASTA and BLAST (18, 19). Sequence alignments were made with Clustal W (accessible via Internet site: http://www2.ebi.ac.uk/clustalw/) and GCG (Framealign, Gap) (20). The GCG package wa ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... The first work comprises the isolation and cDNA cloning of a new carboxypeptidase inhibitor from ticks, named TCI. The recombinant form of this protein is extensively characterized in terms of stability and function, and its possible biological activity is discussed. This work was done in part in Mu ...
... The first work comprises the isolation and cDNA cloning of a new carboxypeptidase inhibitor from ticks, named TCI. The recombinant form of this protein is extensively characterized in terms of stability and function, and its possible biological activity is discussed. This work was done in part in Mu ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Tumor necrosis factor alpha ...
... Tumor necrosis factor alpha ...
Comparative Sequence Analysis of the Genomic Segment 6 of Four
... RNA encodes a protein of" 397 amino acids in all strains with the exception of strain H-2 in which it encodes a protein of 399 amino acids. Alignment of amino acid sequences of the VP6 protein of strain FI-14 and subgroup II rotaviruses (Wa and Gottfried) indicates a high degree of homology (94~), w ...
... RNA encodes a protein of" 397 amino acids in all strains with the exception of strain H-2 in which it encodes a protein of 399 amino acids. Alignment of amino acid sequences of the VP6 protein of strain FI-14 and subgroup II rotaviruses (Wa and Gottfried) indicates a high degree of homology (94~), w ...
Staphylococcus haemolyticus lipase
... chain length selectivity was de¢ned by elements within the region of residues 180^250 in SHL [10]. We isolated a S. haemolyticus strain producing an extracellular lipase from sewage treatment plants in Korea. In this paper the lipase enzyme was puri¢ed and its biochemical properties were studied. In ...
... chain length selectivity was de¢ned by elements within the region of residues 180^250 in SHL [10]. We isolated a S. haemolyticus strain producing an extracellular lipase from sewage treatment plants in Korea. In this paper the lipase enzyme was puri¢ed and its biochemical properties were studied. In ...
From Sequence to Structure
... and to accept hydrogen bonds simultaneously. Cysteine, like histidine, is commonly found in enzyme active sites, because the thiolate anion is the most powerful nucleophile available from the naturally occurring amino acids. Amphipathic residues have both polar and nonpolar character, making them id ...
... and to accept hydrogen bonds simultaneously. Cysteine, like histidine, is commonly found in enzyme active sites, because the thiolate anion is the most powerful nucleophile available from the naturally occurring amino acids. Amphipathic residues have both polar and nonpolar character, making them id ...
Homology modeling
Homology modeling, also known as comparative modeling of protein, refers to constructing an atomic-resolution model of the ""target"" protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein (the ""template""). Homology modeling relies on the identification of one or more known protein structures likely to resemble the structure of the query sequence, and on the production of an alignment that maps residues in the query sequence to residues in the template sequence. It has been shown that protein structures are more conserved than protein sequences amongst homologues, but sequences falling below a 20% sequence identity can have very different structure.Evolutionarily related proteins have similar sequences and naturally occurring homologous proteins have similar protein structure.It has been shown that three-dimensional protein structure is evolutionarily more conserved than would be expected on the basis of sequence conservation alone.The sequence alignment and template structure are then used to produce a structural model of the target. Because protein structures are more conserved than DNA sequences, detectable levels of sequence similarity usually imply significant structural similarity.The quality of the homology model is dependent on the quality of the sequence alignment and template structure. The approach can be complicated by the presence of alignment gaps (commonly called indels) that indicate a structural region present in the target but not in the template, and by structure gaps in the template that arise from poor resolution in the experimental procedure (usually X-ray crystallography) used to solve the structure. Model quality declines with decreasing sequence identity; a typical model has ~1–2 Å root mean square deviation between the matched Cα atoms at 70% sequence identity but only 2–4 Å agreement at 25% sequence identity. However, the errors are significantly higher in the loop regions, where the amino acid sequences of the target and template proteins may be completely different.Regions of the model that were constructed without a template, usually by loop modeling, are generally much less accurate than the rest of the model. Errors in side chain packing and position also increase with decreasing identity, and variations in these packing configurations have been suggested as a major reason for poor model quality at low identity. Taken together, these various atomic-position errors are significant and impede the use of homology models for purposes that require atomic-resolution data, such as drug design and protein–protein interaction predictions; even the quaternary structure of a protein may be difficult to predict from homology models of its subunit(s). Nevertheless, homology models can be useful in reaching qualitative conclusions about the biochemistry of the query sequence, especially in formulating hypotheses about why certain residues are conserved, which may in turn lead to experiments to test those hypotheses. For example, the spatial arrangement of conserved residues may suggest whether a particular residue is conserved to stabilize the folding, to participate in binding some small molecule, or to foster association with another protein or nucleic acid. Homology modeling can produce high-quality structural models when the target and template are closely related, which has inspired the formation of a structural genomics consortium dedicated to the production of representative experimental structures for all classes of protein folds. The chief inaccuracies in homology modeling, which worsen with lower sequence identity, derive from errors in the initial sequence alignment and from improper template selection. Like other methods of structure prediction, current practice in homology modeling is assessed in a biennial large-scale experiment known as the Critical Assessment of Techniques for Protein Structure Prediction, or CASP.