Fibrous Proteins
... (3) Secondary structure of elastin A different type of helix structure from those in the a-helix is present. This is able to stretch and relax like a coiled spring. So elastin is elastic! This is constructed from a helix of repeated b-turns based on the sequence Val.Pro.Gly.Val, and is called the b- ...
... (3) Secondary structure of elastin A different type of helix structure from those in the a-helix is present. This is able to stretch and relax like a coiled spring. So elastin is elastic! This is constructed from a helix of repeated b-turns based on the sequence Val.Pro.Gly.Val, and is called the b- ...
Matricellular Protein Cyr61 Bridges Lysophosphatidic Acid and
... the nuclear receptor peroxisome proliferator-activated receptor (PPAR␥) and induces PPAR␥-dependent gene expression (13). In the present study, the specific PPAR␥ antagonist and primary SMCs isolated from wild type and LPA receptor knock-out mice were used to determine the specific role of LPA recep ...
... the nuclear receptor peroxisome proliferator-activated receptor (PPAR␥) and induces PPAR␥-dependent gene expression (13). In the present study, the specific PPAR␥ antagonist and primary SMCs isolated from wild type and LPA receptor knock-out mice were used to determine the specific role of LPA recep ...
A 5000-Fold Increase in the Specificity of a Bacterial
... Phosphotriesterases (PTEs) have been isolated from a range of bacterial species, including Agrobcaterium radiobacter (PTEAr), and are efficient enzymes with broad substrate ranges. The turnover rate of PTEAr for the common organophosphorous insecticide malathion is lower than expected based on its p ...
... Phosphotriesterases (PTEs) have been isolated from a range of bacterial species, including Agrobcaterium radiobacter (PTEAr), and are efficient enzymes with broad substrate ranges. The turnover rate of PTEAr for the common organophosphorous insecticide malathion is lower than expected based on its p ...
The Primary Structure of the Calcium Ion
... initial fractionation on the Sephadex G-50 column is shown in Fig. 1. The material eluted close to the void volume (fraction A + B) was resolved on a column of Sephadex G-100 into fraction A (at the void volume) and fraction B, as shown in Fig. 2. The peptides in fraction A were aggregated in aqueou ...
... initial fractionation on the Sephadex G-50 column is shown in Fig. 1. The material eluted close to the void volume (fraction A + B) was resolved on a column of Sephadex G-100 into fraction A (at the void volume) and fraction B, as shown in Fig. 2. The peptides in fraction A were aggregated in aqueou ...
Chlamydia Exploit the Mammalian Tryptophan-Depletion
... exported virulence factor that interacts with the host system under conditions of rapid growth. In ...
... exported virulence factor that interacts with the host system under conditions of rapid growth. In ...
C H A P
... extract was carried out by affinity chromatography using NTA-Ni resin, and the purified proteins were recovered and analyzed by SDS-PAGE and zymogram analysis before removing its His-tail. However, no properly purified protein was obtained by this system. For this reason, EstV purification was also ...
... extract was carried out by affinity chromatography using NTA-Ni resin, and the purified proteins were recovered and analyzed by SDS-PAGE and zymogram analysis before removing its His-tail. However, no properly purified protein was obtained by this system. For this reason, EstV purification was also ...
Organic Chemistry/Fourth Edition: e-Text
... charged sulfate groups are exposed to the water. The SDS molecules that they carry ensure that all the protein molecules are negatively charged and migrate toward the positive electrode. Furthermore, all the proteins in the mixture now have similar shapes and tend to travel at rates proportional to ...
... charged sulfate groups are exposed to the water. The SDS molecules that they carry ensure that all the protein molecules are negatively charged and migrate toward the positive electrode. Furthermore, all the proteins in the mixture now have similar shapes and tend to travel at rates proportional to ...
Growth-Environment Dependent Modulation of
... means of the values from three separate batches of cells under each condition. The standard ...
... means of the values from three separate batches of cells under each condition. The standard ...
Phosphorylation of the F1Fo ATP Synthase Я Subunit
... wild-type and had a corresponding increase of lower-molecular-weight forms of the protein, indicating an assembly/stability defect. In addition, the ATPase activity was reduced but not abolished with the phosphomimetic mutation at T58, a site that altered the formation/maintenance of dimers of the F ...
... wild-type and had a corresponding increase of lower-molecular-weight forms of the protein, indicating an assembly/stability defect. In addition, the ATPase activity was reduced but not abolished with the phosphomimetic mutation at T58, a site that altered the formation/maintenance of dimers of the F ...
Molecular forms of prostate specific antigen (PSA) in serum: clinical
... Standards and development of tPSA-assays designed to detect free PSA and PSA-ACT on an equimolar basis, inter-assay variability has decreased – in particular regarding tPSA values. Nevertheless results of commercially available tPSA assays are not yet interchangeable, not uniformly standardised, and ...
... Standards and development of tPSA-assays designed to detect free PSA and PSA-ACT on an equimolar basis, inter-assay variability has decreased – in particular regarding tPSA values. Nevertheless results of commercially available tPSA assays are not yet interchangeable, not uniformly standardised, and ...
Purification and properties of NADP +-dependent
... enzyme. Only at pH 7.5, when already in an almost purified state, did the enzyme show weak binding. In the crude extract however, other proteins seem to displace the glycerol dehydrogenase from the column. ...
... enzyme. Only at pH 7.5, when already in an almost purified state, did the enzyme show weak binding. In the crude extract however, other proteins seem to displace the glycerol dehydrogenase from the column. ...
Introduction to Nutrition and Metabolism, Third Edition
... that work. As discussed in section 5.1, the energy used in various activities can readily be measured, as can the metabolic energy yield of the foods that are the fuel for that work (see Table 1.1). This means that it is possible to calculate a balance between the intake of energy, as metabolic fuel ...
... that work. As discussed in section 5.1, the energy used in various activities can readily be measured, as can the metabolic energy yield of the foods that are the fuel for that work (see Table 1.1). This means that it is possible to calculate a balance between the intake of energy, as metabolic fuel ...
Transcription factor Sp3 is silenced through - IMT
... Recently, we have identi®ed a single lysine residue within the ID of Sp3 that is essential for silencing the transcriptional activity of Sp3 in cell culture experiments (Braun et al., 2001). In the course of these studies we realized that this lysine lies within a motif (IKEE, amino acids 422±425 ac ...
... Recently, we have identi®ed a single lysine residue within the ID of Sp3 that is essential for silencing the transcriptional activity of Sp3 in cell culture experiments (Braun et al., 2001). In the course of these studies we realized that this lysine lies within a motif (IKEE, amino acids 422±425 ac ...
Plant aromatic amino acid decarboxylases
... divergent AAAD-like proteins. For example, an expansion of the AAAD gene within insect and plant species results in a multiplicity of functionally diverse AAAD enzymes. A single AAAD annotation does not accurately represent the selection of activities and substrate specificities. This can be illustr ...
... divergent AAAD-like proteins. For example, an expansion of the AAAD gene within insect and plant species results in a multiplicity of functionally diverse AAAD enzymes. A single AAAD annotation does not accurately represent the selection of activities and substrate specificities. This can be illustr ...
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA
... pathogen responses. The relationship between mRNA levels and relative amounts of selected secretome proteins was assessed. The results indicate that transcriptional control is but one of many factors contributing to Arabidopsis Pi starvation responses and highlight the importance of parallel biochem ...
... pathogen responses. The relationship between mRNA levels and relative amounts of selected secretome proteins was assessed. The results indicate that transcriptional control is but one of many factors contributing to Arabidopsis Pi starvation responses and highlight the importance of parallel biochem ...
CHRYSAORA QUINQUECIRRHA) Research Article
... such as collagen, attract water in the stratum corneum. In contrast to low molecular weight humectants, such as urea or glycerine, humectants of higher molecular weight, such as collagen, are not absorbed by the stratum corneum but rather stay on the skin surface, where they bind water by hydration ...
... such as collagen, attract water in the stratum corneum. In contrast to low molecular weight humectants, such as urea or glycerine, humectants of higher molecular weight, such as collagen, are not absorbed by the stratum corneum but rather stay on the skin surface, where they bind water by hydration ...
Nucleotide sequence and structural organization of
... Southern hybridizations. After fractionation on 0.7 YOagarose gels, DNA was transferred to nitrocellulose filters by the merhod of Southern (1975). D N A was labelled using the enhanced-chemiluminance (ECL) gene detection system (Amersham). Probe labelling, hybridization and washing steps were done ...
... Southern hybridizations. After fractionation on 0.7 YOagarose gels, DNA was transferred to nitrocellulose filters by the merhod of Southern (1975). D N A was labelled using the enhanced-chemiluminance (ECL) gene detection system (Amersham). Probe labelling, hybridization and washing steps were done ...
METABOLIC CUES AND REGULATORY PROTEINS
... establish an intracellular niche protected from digestion. Moreover, if humans inhale bacteria-laden aerosols, L. pneumophila can survive and replicate within alveolar macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates ...
... establish an intracellular niche protected from digestion. Moreover, if humans inhale bacteria-laden aerosols, L. pneumophila can survive and replicate within alveolar macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
... vesicle-associated membrane protein 2(VAMP2) or synaptobrevin present in synaptic vesicle and synaptosomalassociated protein of 25 kDa (SNAP25) present in plasma membrane respectively 3. Due to this fatal neurological action, botulinum toxin is a potent bio-weapon for spreading terrorism and poses a ...
... vesicle-associated membrane protein 2(VAMP2) or synaptobrevin present in synaptic vesicle and synaptosomalassociated protein of 25 kDa (SNAP25) present in plasma membrane respectively 3. Due to this fatal neurological action, botulinum toxin is a potent bio-weapon for spreading terrorism and poses a ...
CHAPTER 15 - GLYCOGEN METABOLISM AND
... meet ATP needs during exertion. Glycogen breakdown Glycogen consists of glucose residues linked by " 1,4 linkages with " 1,6 branches every 10 residues or so. (See Figure 2 a). The breakdown of glycogen can be though of as a minipathway that intersects glycolysis at glucose-6-phosphate Glycogen is c ...
... meet ATP needs during exertion. Glycogen breakdown Glycogen consists of glucose residues linked by " 1,4 linkages with " 1,6 branches every 10 residues or so. (See Figure 2 a). The breakdown of glycogen can be though of as a minipathway that intersects glycolysis at glucose-6-phosphate Glycogen is c ...
DECALEPIS HAMILTONII ACETAMINOPHEN-INDUCED HEPATIC INJURY IN RATS Research Article
... Values are expressed as mean ± SD of six rats in each group. ANOVA followed by Duncan’s multiple range tests. Values not sharing a common superscript differ significantly at P ≤ 0.05. The mean levels of LPO and weight in the hepatic tissue of group II (acetaminophen -induced) rats were significantly ...
... Values are expressed as mean ± SD of six rats in each group. ANOVA followed by Duncan’s multiple range tests. Values not sharing a common superscript differ significantly at P ≤ 0.05. The mean levels of LPO and weight in the hepatic tissue of group II (acetaminophen -induced) rats were significantly ...
Susan Viggiano and Michael S. Cosgrove
... times and then visualized by chemiluminescence (Clarity Western, Bio-Rad) on a Bio-Rad ChemiDoc MP Imager using the chemiluminescence setting. For pulldown assays using cell extracts, a 3 M concentration of GST-tagged SET domains was incubated with 100 g of MCF-7 cell extracts for 16 h at 4 °C. Fo ...
... times and then visualized by chemiluminescence (Clarity Western, Bio-Rad) on a Bio-Rad ChemiDoc MP Imager using the chemiluminescence setting. For pulldown assays using cell extracts, a 3 M concentration of GST-tagged SET domains was incubated with 100 g of MCF-7 cell extracts for 16 h at 4 °C. Fo ...
Glyphosate pathways to modern diseases V: Amino
... having functionality resembling the activities of cationic antimicrobial peptides [8]. A reactive side chain is attached to the nitrogen of glycine, and such units can be assembled into “peptoid” chains that in many ways resemble peptide chains, except that they are highly resistant to proteolysis. ...
... having functionality resembling the activities of cationic antimicrobial peptides [8]. A reactive side chain is attached to the nitrogen of glycine, and such units can be assembled into “peptoid” chains that in many ways resemble peptide chains, except that they are highly resistant to proteolysis. ...
Analysis of the bipartite networks of domain compositions and
... It is widely accepted that complexity of biological systems arises from combinations of common subunits. In this work we investigate the combinatorial patterns of protein domains in the metabolic networks and find several general rules in the patterns of domain combinations and their evolution. Firs ...
... It is widely accepted that complexity of biological systems arises from combinations of common subunits. In this work we investigate the combinatorial patterns of protein domains in the metabolic networks and find several general rules in the patterns of domain combinations and their evolution. Firs ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.