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DEPARTMENT OF CHEMISTRY TEACHING LAB EXPERIMENT RISK ASSESSMENT FORM This form must be completed jointly by the Lab Officer in charge and the Lecturer in charge. A hardcopy of the completed form should be kept in a file together with the Project Risk Assessment. Name of Lecturer in Charge Name of Lab Officer in Charge Module / Expt No. Prof Sam Li Activity being assessed: Ms Tang CN CM2142 / 7 Gel electrophoresis Known or expected hazards associated with the activity: Hazards of reagents, solvents and known reaction products. State each substance and the approximate amounts to be used/produced. See Appendix 2.7 Incompatible materials (special precautions): Keep high voltage equipment away from water source. The risk of injury and its severity likely to arise from these hazards: High voltage used. Risk : fatal electric shock. Who is at risk? Person performing the experiment. Measure to be taken to reduce the level of risk: Keep hands dry when handling electrical equipment. Switch off mains before plugging or unplugging wires into the power supply. Wear rubber soled shoes. Eye protection to be worn. Training prerequisites: Supervision required. Use of Standard Electrical Equipment Level of risk remaining: Low. Emergency action if : Spill: (i) (ii) (iii) Eyes : Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin : Wash affected area with water for 10mins. Remove contaminated clothing. Consult physician if necessary. Bench: Spillages on the work bench should be mopped up with a piece of cloth immediately. Fire: use CO2 fire extinguisher. Is the experiment suitable for out-of-hours operation ? References if any: Refer to the following MSDS for more information Agarose : http://hazard.com/msds/f2/cjq/cjqvp.html Tris borate EDTA buffer : http://hazard.com/msds/f2/ckb/ckbpy.html Page 1 of 4 Printed on: 27 June 2017 Yes No Bromophenol blue : http://www.jtbaker.com/msds/englishhtml/b5056.htm Methylene blue : http://www.jtbaker.com/msds/englishhtml/m4381.htm Orange G : http://www.jtbaker.com/msds/englishhtml/o4140.htm Safranin O : http://www.jtbaker.com/msds/englishhtml/s0240.htm Xylene cyanol : https://fscimage.fishersci.com/msds/89336.htm Glycerol: http://www.uiowa.edu/~chemsafe/MSDS/Glcerol.htm Signature of Lab Officer in Charge:……………………………………………………………….. Date:………………………… Signature of Lecturer in Charge:………… …………………………………….. Date:… …………………….. Prepared Risks Assessments for standard equipment and operation are with the kind permission of Dr. Ken MacNeil, School of Chemistry, University of Bristol. Page 2 of 4 Printed on: 27 June 2017 Activity being assessed: Note any activity to be used which entail risk (e.g. use of glass vacuum apparatus, high pressures, high voltage, radiation, high temperatures). Give reference to any special protocols to be followed, and if appropriate attach copies to the risk assessment form. State any additional precautions taken to minimise risk. Known or expected hazards associated with the activity: FOR EACH CHEMICAL, read the MSDS and note:a) Particular hazards (e.g. highly toxic, carcinogenic, corrosive, flammable, pyrophoric, explosive, volatile, dust hazard). Note any dangerous combinations of properties (e.g. volatile and toxic). b) Requirements for safe handling (e.g. fume cupboard, inert atmosphere, low temperature). c) How to dispose of residuals Dispose to drain, with water dilution Neutralise, then to drain with suitable dilution To flammable liquid waste receptacle To non-flammable liquid waste receptacle Keep for recovery/recycling Keep for special disposal later (e.g. heavy metals) Double bag and dispose to dry waste Special procedure (specify) Incompatible materials (special precautions) Note any dangerously incompatible materials and hazards arising from contact of any reagents and substances used with common materials such as paper, benches, hoses, etc. Measures to be taken to reduce the level of risk Include hazards of previously unknown products. Location of work – laboratory, open bench, fume cupboard Level of risk remaining: Likelihood and consequences of any accident or unforeseen events whilst carrying out the activity. When this has been done, choose the appropriate procedure:a) Close supervision and/or attendance of trained first-aider needed. b) Specific approval of supervisor needed. c) Training is needed prior-to or during the operations specified. d) Training is complete and only general laboratory competence required. e) No risk perceived. Emergency action: a) Any special requirements to deal with accidental spillage or leakage. b) What to do in the event of accidental exposure (skin contact, inhalation, etc.). Page 3 of 4 Printed on: 27 June 2017 Appendix 2.7 Reagent Agarose Concentration 1.5% in TBE Amount used/produced 100mL TBE : 108g Tris base + 55g Boric acid + 3.9g EDTA Bromophenol blue Methylene blue 0.5x dilution 500mL May cause central nervous system effects 0.25% w/v in 50% glycerol 0.25% w/v in 50% glycerol 0.25% w/v in 50% glycerol 5μL May cause irritation. 5μL Eye irritation 5μL Safranin O 0.25% w/v in 50% glycerol 5μL Xylene cyanol 0.25% w/v in 50% glycerol 5μL Maybe harmful if inhaled, swallowed or absorbed through skin Eye contact may result in permanent eye damage. Causes eyes and skin irritation. May cause irritation. Orange G Dye mixture Page 4 of 4 Printed on: 27 June 2017 10μL Expected hazards May be harmful by inhalation, ingestion or skin absorption. Treatment/Disposal Place used agar in “Used Agarose” container for recycling. Place used TBE in “Used TBE” container for recycling. Wash spillages away with plenty of water. Wash spillages away with plenty of water. Wash spillages away with plenty of water. Wash spillages away with plenty of water. Wash spillages away with plenty of water. Wash spillages away with plenty of water.