Download DEPARTMENT OF CHEMISTRY TEACHING LAB EXPERIMENT

DEPARTMENT OF CHEMISTRY
TEACHING LAB EXPERIMENT RISK ASSESSMENT FORM
This form must be completed jointly by the Lab Officer in charge and the Lecturer in charge. A
hardcopy of the completed form should be kept in a file together with the Project Risk
Assessment.
Name of Lecturer in Charge
Name of Lab Officer in Charge
Module / Expt No.
Prof Sam Li
Activity being assessed:
Ms Tang CN
CM2142 / 7
Gel electrophoresis
Known or expected hazards associated with the activity:
Hazards of reagents, solvents and known reaction products.
State each substance and the approximate amounts to be used/produced.
See Appendix 2.7
Incompatible materials (special precautions):
Keep high voltage equipment away from water source.
The risk of injury and its severity likely to arise from these hazards:
High voltage used.
Risk : fatal electric shock.
Who is at risk?
Person performing the experiment.
Measure to be taken to reduce the level of risk:
Keep hands dry when handling electrical equipment. Switch off mains before plugging or unplugging
wires into the power supply. Wear rubber soled shoes. Eye protection to be worn.
Training prerequisites:
Supervision required.
Use of Standard Electrical Equipment
Level of risk remaining:
Low.
Emergency action if :
Spill:
(i)
(ii)
(iii)
Eyes : Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the
upper and lower eyelids. Get medical aid.
Skin : Wash affected area with water for 10mins. Remove contaminated clothing.
Consult physician if necessary.
Bench: Spillages on the work bench should be mopped up with a piece of cloth
immediately.
Fire: use CO2 fire extinguisher.
Is the experiment suitable for out-of-hours operation ?
References if any: Refer to the following MSDS for more information
Agarose : http://hazard.com/msds/f2/cjq/cjqvp.html
Tris borate EDTA buffer : http://hazard.com/msds/f2/ckb/ckbpy.html
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Yes
No
Bromophenol blue : http://www.jtbaker.com/msds/englishhtml/b5056.htm
Methylene blue : http://www.jtbaker.com/msds/englishhtml/m4381.htm
Orange G : http://www.jtbaker.com/msds/englishhtml/o4140.htm
Safranin O : http://www.jtbaker.com/msds/englishhtml/s0240.htm
Xylene cyanol : https://fscimage.fishersci.com/msds/89336.htm
Glycerol: http://www.uiowa.edu/~chemsafe/MSDS/Glcerol.htm
Signature of Lab Officer in Charge:………………………………………………………………..
Date:…………………………
Signature of Lecturer in Charge:………… ……………………………………..
Date:… ……………………..
Prepared Risks Assessments for standard equipment and operation are with the kind permission of Dr. Ken MacNeil,
School of Chemistry, University of Bristol.
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Activity being assessed:
Note any activity to be used which entail risk (e.g. use of glass vacuum apparatus, high pressures, high
voltage, radiation, high temperatures). Give reference to any special protocols to be followed, and if
appropriate attach copies to the risk assessment form. State any additional precautions taken to minimise
risk.
Known or expected hazards associated with the activity:
FOR EACH CHEMICAL, read the MSDS and note:a) Particular hazards (e.g. highly toxic, carcinogenic, corrosive, flammable, pyrophoric, explosive, volatile,
dust hazard). Note any dangerous combinations of properties (e.g. volatile and toxic).
b) Requirements for safe handling (e.g. fume cupboard, inert atmosphere, low temperature).
c) How to dispose of residuals
 Dispose to drain, with water dilution
 Neutralise, then to drain with suitable dilution
 To flammable liquid waste receptacle
 To non-flammable liquid waste receptacle
 Keep for recovery/recycling
 Keep for special disposal later (e.g. heavy metals)
 Double bag and dispose to dry waste
 Special procedure (specify)
Incompatible materials (special precautions)
Note any dangerously incompatible materials and hazards arising from contact of any reagents and
substances used with common materials such as paper, benches, hoses, etc.
Measures to be taken to reduce the level of risk
Include hazards of previously unknown products.
Location of work – laboratory, open bench, fume cupboard
Level of risk remaining:
Likelihood and consequences of any accident or unforeseen events whilst carrying out the activity. When
this has been done, choose the appropriate procedure:a) Close supervision and/or attendance of trained first-aider needed.
b) Specific approval of supervisor needed.
c) Training is needed prior-to or during the operations specified.
d) Training is complete and only general laboratory competence required.
e) No risk perceived.
Emergency action:
a) Any special requirements to deal with accidental spillage or leakage.
b) What to do in the event of accidental exposure (skin contact, inhalation, etc.).
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Appendix 2.7
Reagent
Agarose
Concentration
1.5% in TBE
Amount
used/produced
100mL
TBE : 108g
Tris base + 55g
Boric acid +
3.9g EDTA
Bromophenol
blue
Methylene blue
0.5x dilution
500mL
May cause central
nervous system effects
0.25% w/v in
50% glycerol
0.25% w/v in
50% glycerol
0.25% w/v in
50% glycerol
5μL
May cause irritation.
5μL
Eye irritation
5μL
Safranin O
0.25% w/v in
50% glycerol
5μL
Xylene cyanol
0.25% w/v in
50% glycerol
5μL
Maybe harmful if
inhaled, swallowed or
absorbed through skin
Eye contact may result
in permanent eye
damage.
Causes eyes and skin
irritation.
May cause irritation.
Orange G
Dye mixture
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10μL
Expected hazards
May be harmful by
inhalation, ingestion or
skin absorption.
Treatment/Disposal
Place used agar in
“Used Agarose”
container for recycling.
Place used TBE in
“Used TBE” container
for recycling.
Wash spillages away
with plenty of water.
Wash spillages away
with plenty of water.
Wash spillages away
with plenty of water.
Wash spillages away
with plenty of water.
Wash spillages away
with plenty of water.
Wash spillages away
with plenty of water.