Download DEPARTMENT OF CHEMISTRY TEACHING LAB EXPERIMENT

DEPARTMENT OF CHEMISTRY
TEACHING LAB EXPERIMENT RISK ASSESSMENT FORM
This form must be completed jointly by the Lab Officer in charge and the Lecturer in charge. A
hardcopy of the completed form should be kept in a file together with the Project Risk
Assessment.
Name of Lecturer in Charge
Name of Lab Officer in Charge
Module / Expt No.
Dr Thyarajan Saradha
Activity being assessed:
Ms Ong Bee Hoon
CM3292 / Expt. 3.3
Capillary Electrophoresis
Known or expected hazards associated with the activity:
Hazards of reagents, solvents and known reaction products.
State each substance and the approximate amounts to be used/produced.
See Appendix 3.3
Incompatible materials (special precautions):
NaCl: Lithium, bromine trifluoride.
NaNO3 : Cyanides, combustible material, strong reducing agents, aluminium .
Na2SO4: Strong acids, aluminium, magnesium, strong bases.
CHES: Incompatible with strong oxidizing agents.
CTAB: Incompatible with strong oxidizing agents.
K2Cr2O7: Incompatible with combustible materials, organic materials, strong reducing agents.
Brij35: Avoid strong oxidizing agents.
The risk of injury and its severity likely to arise from these hazards:
There is potential for electrical shock from the high voltage power supply.
Who is at risk?
Person performing the experiment.
Measure to be taken to reduce the level of risk:
Personal protection equipment such as safety glasses and lab coat are to be worn. Avoid water.
Training prerequisites:
General laboratory competency.
Use of Glassware
Use of Standard Electrical Equipment
Level of risk remaining:
Low.
Emergency action if :
Spill: wash affected area with plenty of water, remove contaminated clothing, seek medical help
if necessary. Spillages on the work bench should be mopped up with a wet cloth immediately.
Fire: evacuate room, use a CO2 fire extinguisher.
Is the experiment suitable for out-of-hours operation ?
References if any:
Sodium chloride : http://avogadro.chem.iastate.edu/MSDS/NaCl.htm
Sodium nitrate : http://www.jtbaker.com/msds/englishhtml/s4442.htm
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Yes
No
Sodium Bromate: http://fscimage.fishersci.com/msds/21050.htm
CHES : http://hazard.com/msds/mf/baker/baker/files/c2089.htm
CTAB : http://www.sciencelab.com/page/S/PVAR/10415/SLH2563
Potassium Dichromate : http://www.jtbaker.com/msds/englishhtml/p5719.htm
Brij35 : http://www.sciencelab.com/page/S/PVAR/SLB1294
Signature of Lab Officer in Charge:………………………………………………………………..
Date:…………………………
Signature of Lecturer in Charge:………… ……………………………………..
Date:… ……………………..
Prepared Risks Assessments for standard equipment and operation are with the kind permission of Dr. Ken MacNeil,
School of Chemistry, University of Bristol.
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Activity being assessed:
Note any activity to be used which entail risk (e.g. use of glass vacuum apparatus, high pressures, high
voltage, radiation, high temperatures). Give reference to any special protocols to be followed, and if
appropriate attach copies to the risk assessment form. State any additional precautions taken to minimise
risk.
Known or expected hazards associated with the activity:
FOR EACH CHEMICAL, read the MSDS and note:a) Particular hazards (e.g. highly toxic, carcinogenic, corrosive, flammable, pyrophoric, explosive, volatile,
dust hazard). Note any dangerous combinations of properties (e.g. volatile and toxic).
b) Requirements for safe handling (e.g. fume cupboard, inert atmosphere, low temperature).
c) How to dispose of residuals
 Dispose to drain, with water dilution
 Neutralise, then to drain with suitable dilution
 To flammable liquid waste receptacle
 To non-flammable liquid waste receptacle
 Keep for recovery/recycling
 Keep for special disposal later (e.g. heavy metals)
 Double bag and dispose to dry waste
 Special procedure (specify)
Incompatible materials (special precautions)
Note any dangerously incompatible materials and hazards arising from contact of any reagents and
substances used with common materials such as paper, benches, hoses, etc.
Measures to be taken to reduce the level of risk
Include hazards of previously unknown products.
Location of work – laboratory, open bench, fume cupboard
Level of risk remaining:
Likelihood and consequences of any accident or unforeseen events whilst carrying out the activity. When
this has been done, choose the appropriate procedure:a) Close supervision and/or attendance of trained first-aider needed.
b) Specific approval of supervisor needed.
c) Training is needed prior-to or during the operations specified.
d) Training is complete and only general laboratory competence required.
e) No risk perceived.
Emergency action:
a) Any special requirements to deal with accidental spillage or leakage.
b) What to do in the event of accidental exposure (skin contact, inhalation, etc.).
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Appendix 3.12
Reagent
Concentration
Amount
used/produced
Expected hazards
Cl- (Na salt)
500ppm
10mL
None.
-
NO3 (Na salt)
-
BrO3 (Na salt)
CE buffer (pH 8.1)
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500ppm
500ppm
20mM CHES
50uM CTAB
2.5mM K2Cr2O7
0.17% Brij35
pH 8.1
May cause irritation.
1mL
May cause irritation.
Irritant
Poison
Carcinogenic.