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Last Revision: Fall 2014-2015 SYLLABUS BIO 220 4 Semester Credit Hours - B I. Microbiology 6 Contact Hours Course Description This course covers fundamental principles of microbiology including history of Microbiology, cell structure and function, microbial genetics, infectious diseases, and Immunity. Emphasized topics are distribution, physiology, culture, identification, classification, and disease control of microorganisms. Two 120 minute laboratory experiences are required. II. Prerequisite None III. Course Textbooks, Manuals, or Other Required Materials A. B. IV. Cowen, Marjorie Kelly. Microbiology Fundamentals: A Clinical Approach, 1st edition McGraw-Hill, 2013. Brown, Alfred E., Benson’s Microbiological Applications, 13th edition Short Version. New York, New York. McGraw Hill Publishers, 2015. Course Learning Outcomes At the end of the course the student will develop an understanding of concepts, develop competent use of knowledge in the following areas: A. History of microbiology B. Microbial cell structure and function. C. Microbial growth and control of microbial growth. D. Microbial genetics. E. Immunity. F. Infectious Diseases. G. Appropriate laboratory techniques. V. Outline of Course Topics A. Lecture Topics 1. History of Microbiology a. Theory of Spontaneous Generation b. Vital Activities of Microorganisms c. Applications of Microbiology d. Medical Microbiology 2. 3. 4. 5. 6. e. The Domains: Bacteria, Archaea, and Eucarya f. Viruses, Viroids, and Prions Microscopy and Cell Structure a. Microscopes: The Instruments b. Microscopic Techniques: Dyes and Staining c. Morphology of Prokaryotic Cells i. Cytoplasmic Membrane ii. Movement of Molecules Across the Cytoplasmic Membrane iii. Cell Wall iv. Cell Wall Appendages: Flagella, Pili v. Internal Structures of a Prokaryote d. Morphology of Eukaryotic Cells i. Plasma Membrane ii. Protein Structures: Cytoskeleton, Flagella, and Cilia iii. Membrane Bound Organelles: Nucleus, Mitochondria, Chloroplasts, Endoplasmic Reticulum, Golgi, Lysosomes, and Peroxisomes Metabolism: Fueling Cell Growth a. Principles of Metabolism b. Enzymes c. Central Metabolic Pathways d. Respiration e. Fermentation f. Catabolism of Organic Compounds other than Glucose g. Photosynthesis h. Anabolism Pathways: Subunit Synthesis Prokaryote Growth a. Pure Cultures b. Principles of Bacterial Growth c. Environmental Factors that Influence Microbial Growth d. Nutritional Factors that Influence Microbial Growth e. Laboratory Cultivation of Prokaryotes f. Methods to Detect and Measure Bacterial Growth g. Bacterial Growth in Laboratory Conditions h. Bacterial Growth in Nature Control of Microbial Growth a. Principles of Control of Growth b. Selection of an Antimicrobial Procedure c. Using Heat to Destroy Microorganisms and Viruses d. Using Chemcials to Destroy Microorganisms and Viruses e. Removal of Microorganisms by Filtration f. Using Radiation to Destroy Microorganisms and Viruses g. Preservation to Decrease Growth of Spoilage Organisms Bacterial Genetics a. Spontaneous Mutations 7. 8. 9. 10. 11. 12. b. Induced Mutations c. Repair of Damaged DNA d. Mutations and Their Consequences e. Mutant Selection f. Transformation g. Transduction h. Conjugation i. Plasmids j. Transposable Elements k. Importance of Gene Transfer to Bacteria Biotechnology and Recombinant DNA a. Applications of Genetic Engineering b. Applications of Nucleic Acid Hybridization c. Applications of DNA Sequencing d. Techniques Used in Genetic Engineering e. Techniques Used in Nucleic Acid Hybridization f. Techniques Used in DNA Sequencing g. Techniques Used in the Polymerase Chain Reaction (PCR) Epidemiology a. Principles of Epidemiology b. Epidemiological Studies c. Infectious Disease Surveillance d. Trends in Disease e. Nosocomial Infections Skin Infections a. Anatomy and Physiology of the Skin b. Normal Flora of the Skin c. Bacterial Skin Diseases d. Viral Skin Diseases e. Fungal Skin Diseases Respiratory System Infections a. Anatomy and Physiology of the Respiratory System b. Normal Flora of the Respiratory System c. Bacterial and Viral Infections of the Upper Respiratory System d. Bacterial and Viral Infections of the Lower Respiratory System Alimentary System Infections a. Anatomy and Physiology of the Alimentary System b. Normal Flora of the Alimentary System c. Bacterial and Viral Diseases of the Upper Alimentary System d. Bacterial and Viral Diseases of the Lower Alimentary System Genitourinary Infections a. Anatomy and Physiology of the Genitourinary Systems b. Normal Flora of the Genitourinary Systems c. Non-Venereal Genital Diseases d. Sexually Transmitted Diseases i. Bacterial Sexually Transmitted Diseases B. VI. ii. Viral Sexually Transmitted Diseases iii. Protozoal Sexually Transmitted Diseases 13. Nervous System Infections a. Anatomy and Physiology of the Nervous System b. Bacterial Nervous System Infections c. Viral Diseases of the Nervous System d. Fungal Diseases of the Nervous System e. Transmissible Spongiform Encephalopathies 14. Wound Infections a. Anatomy and Physiology of Wound Infections b. Common Bacterial Wound Infections c. Diseases Due to Anaerobic Bacterial Wound Infections d. Bacterial Bite Wound Infections e. Fungal Wound Infections 15. Blood and Lymphatic Infections a. Anatomy and Physiology of the Blood and Lymph Systems b. Bacterial Diseases of the Blood/Vascular System c. Bacterial Diseases of the Lymph Nodes and Spleen d. Viral Diseases of the Lymphoid and Blood Vascular Systems Laboratory Topics 1. Brightfield Microscopy 2. Smear Preparation 3. Simple Staining, Gram Staining, and Acid-fast Staining 4. Motility Determination 5. Culture Media Preparation 6. Aseptic Technique 7. Pure Culture Techniques 8. Isolation of Anaerobes 9. Temperature : Effects on Growth 10. Temperature: Lethal Effects 11. Evaluation of Disinfectants and Antiseptics 12. Antimicrobic Sensitivity Testing 13. Alcohol Evaluation 14. Oligodynamic Action 15. The Staphylococci 16. The Streptococci 17. Gram-Negative Intestinal Pathogens 18. Morphological Study of Unknown and Cultural Characteristics Methods of Instruction A. B. C. D. Lecture/Discussion Microbiology laboratory Power Point Presentations Blackboard (Tutorials/Practice VII. Evaluation and Assessment Procedures for Assessment of Student Achievement: A. Written examinations B. Written quizzes C. Laboratory work D. Comprehensive final E. Grades will be given based upon A = 90 – 100%, B = 80 – 89%, C = 70 – 79%, D = 60 – 69%, and F = below 60%. VIII. Attendance Students are expected to attend all classes for which they are registered. Students who are unable to attend class regularly, regardless of the reason or circumstance, should withdraw from that class before poor attendance interferes with the student’s ability to achieve the objectives required in the course. Withdrawal from class can affect eligibility for federal financial aid. IX. Statement on Discrimination/Harassment NACC and the Alabama State Board of Education are committed to providing both employment and educational environments free of harassment or discrimination related to an individual’s race, color, gender, religion, national origin, age, or disability. Such harassment is a violation of State Board of Education policy. Any practice or behavior that constitutes harassment or discrimination will not be tolerated. X. Statement of Adherence to ADA Guidelines The Rehabilitation Act of 1973 (Section 504) and the Americans with Disabilities Act of 1990 state that qualified students with disabilities who meet the essential functions and academic requirements are entitled to reasonable accommodations. It is the student’s responsibility to provide appropriate disability documentation to the College.