Download BIO 220 General Microbiology

Last Revision: Fall 2014-2015
SYLLABUS
BIO 220
4 Semester Credit Hours - B
I.
Microbiology
6 Contact Hours
Course Description
This course covers fundamental principles of microbiology including history of
Microbiology, cell structure and function, microbial genetics, infectious diseases, and
Immunity. Emphasized topics are distribution, physiology, culture, identification,
classification, and disease control of microorganisms. Two 120 minute laboratory
experiences are required.
II.
Prerequisite
None
III.
Course Textbooks, Manuals, or Other Required Materials
A.
B.
IV.
Cowen, Marjorie Kelly. Microbiology Fundamentals: A Clinical Approach, 1st
edition McGraw-Hill, 2013.
Brown, Alfred E., Benson’s Microbiological Applications, 13th edition Short
Version. New York, New York. McGraw Hill Publishers, 2015.
Course Learning Outcomes
At the end of the course the student will develop an understanding of concepts, develop
competent use of knowledge in the following areas:
A.
History of microbiology
B.
Microbial cell structure and function.
C.
Microbial growth and control of microbial growth.
D.
Microbial genetics.
E.
Immunity.
F.
Infectious Diseases.
G.
Appropriate laboratory techniques.
V.
Outline of Course Topics
A.
Lecture Topics
1.
History of Microbiology
a.
Theory of Spontaneous Generation
b.
Vital Activities of Microorganisms
c.
Applications of Microbiology
d.
Medical Microbiology
2.
3.
4.
5.
6.
e.
The Domains: Bacteria, Archaea, and Eucarya
f.
Viruses, Viroids, and Prions
Microscopy and Cell Structure
a.
Microscopes: The Instruments
b.
Microscopic Techniques: Dyes and Staining
c.
Morphology of Prokaryotic Cells
i.
Cytoplasmic Membrane
ii.
Movement of Molecules Across the Cytoplasmic
Membrane
iii.
Cell Wall
iv.
Cell Wall Appendages: Flagella, Pili
v.
Internal Structures of a Prokaryote
d.
Morphology of Eukaryotic Cells
i.
Plasma Membrane
ii.
Protein Structures: Cytoskeleton, Flagella, and Cilia
iii.
Membrane Bound Organelles: Nucleus, Mitochondria,
Chloroplasts, Endoplasmic Reticulum, Golgi, Lysosomes,
and Peroxisomes
Metabolism: Fueling Cell Growth
a.
Principles of Metabolism
b.
Enzymes
c.
Central Metabolic Pathways
d.
Respiration
e.
Fermentation
f.
Catabolism of Organic Compounds other than Glucose
g.
Photosynthesis
h.
Anabolism Pathways: Subunit Synthesis
Prokaryote Growth
a.
Pure Cultures
b.
Principles of Bacterial Growth
c.
Environmental Factors that Influence Microbial Growth
d.
Nutritional Factors that Influence Microbial Growth
e.
Laboratory Cultivation of Prokaryotes
f.
Methods to Detect and Measure Bacterial Growth
g.
Bacterial Growth in Laboratory Conditions
h.
Bacterial Growth in Nature
Control of Microbial Growth
a.
Principles of Control of Growth
b.
Selection of an Antimicrobial Procedure
c.
Using Heat to Destroy Microorganisms and Viruses
d.
Using Chemcials to Destroy Microorganisms and Viruses
e.
Removal of Microorganisms by Filtration
f.
Using Radiation to Destroy Microorganisms and Viruses
g.
Preservation to Decrease Growth of Spoilage Organisms
Bacterial Genetics
a.
Spontaneous Mutations
7.
8.
9.
10.
11.
12.
b.
Induced Mutations
c.
Repair of Damaged DNA
d.
Mutations and Their Consequences
e.
Mutant Selection
f.
Transformation
g.
Transduction
h.
Conjugation
i.
Plasmids
j.
Transposable Elements
k.
Importance of Gene Transfer to Bacteria
Biotechnology and Recombinant DNA
a.
Applications of Genetic Engineering
b.
Applications of Nucleic Acid Hybridization
c.
Applications of DNA Sequencing
d.
Techniques Used in Genetic Engineering
e.
Techniques Used in Nucleic Acid Hybridization
f.
Techniques Used in DNA Sequencing
g.
Techniques Used in the Polymerase Chain Reaction (PCR)
Epidemiology
a.
Principles of Epidemiology
b.
Epidemiological Studies
c.
Infectious Disease Surveillance
d.
Trends in Disease
e.
Nosocomial Infections
Skin Infections
a.
Anatomy and Physiology of the Skin
b.
Normal Flora of the Skin
c.
Bacterial Skin Diseases
d.
Viral Skin Diseases
e.
Fungal Skin Diseases
Respiratory System Infections
a.
Anatomy and Physiology of the Respiratory System
b.
Normal Flora of the Respiratory System
c.
Bacterial and Viral Infections of the Upper Respiratory System
d.
Bacterial and Viral Infections of the Lower Respiratory System
Alimentary System Infections
a.
Anatomy and Physiology of the Alimentary System
b.
Normal Flora of the Alimentary System
c.
Bacterial and Viral Diseases of the Upper Alimentary System
d.
Bacterial and Viral Diseases of the Lower Alimentary System
Genitourinary Infections
a.
Anatomy and Physiology of the Genitourinary Systems
b.
Normal Flora of the Genitourinary Systems
c.
Non-Venereal Genital Diseases
d.
Sexually Transmitted Diseases
i.
Bacterial Sexually Transmitted Diseases
B.
VI.
ii.
Viral Sexually Transmitted Diseases
iii.
Protozoal Sexually Transmitted Diseases
13.
Nervous System Infections
a.
Anatomy and Physiology of the Nervous System
b.
Bacterial Nervous System Infections
c.
Viral Diseases of the Nervous System
d.
Fungal Diseases of the Nervous System
e.
Transmissible Spongiform Encephalopathies
14.
Wound Infections
a.
Anatomy and Physiology of Wound Infections
b.
Common Bacterial Wound Infections
c.
Diseases Due to Anaerobic Bacterial Wound Infections
d.
Bacterial Bite Wound Infections
e.
Fungal Wound Infections
15.
Blood and Lymphatic Infections
a.
Anatomy and Physiology of the Blood and Lymph Systems
b.
Bacterial Diseases of the Blood/Vascular System
c.
Bacterial Diseases of the Lymph Nodes and Spleen
d.
Viral Diseases of the Lymphoid and Blood Vascular Systems
Laboratory Topics
1.
Brightfield Microscopy
2.
Smear Preparation
3.
Simple Staining, Gram Staining, and Acid-fast Staining
4.
Motility Determination
5.
Culture Media Preparation
6.
Aseptic Technique
7.
Pure Culture Techniques
8.
Isolation of Anaerobes
9.
Temperature : Effects on Growth
10.
Temperature: Lethal Effects
11.
Evaluation of Disinfectants and Antiseptics
12.
Antimicrobic Sensitivity Testing
13.
Alcohol Evaluation
14.
Oligodynamic Action
15.
The Staphylococci
16.
The Streptococci
17.
Gram-Negative Intestinal Pathogens
18.
Morphological Study of Unknown and Cultural Characteristics
Methods of Instruction
A.
B.
C.
D.
Lecture/Discussion
Microbiology laboratory
Power Point Presentations
Blackboard (Tutorials/Practice
VII.
Evaluation and Assessment
Procedures for Assessment of Student Achievement:
A.
Written examinations
B.
Written quizzes
C.
Laboratory work
D.
Comprehensive final
E.
Grades will be given based upon A = 90 – 100%, B = 80 – 89%, C = 70 – 79%, D
= 60 – 69%, and F = below 60%.
VIII. Attendance
Students are expected to attend all classes for which they are registered. Students who are
unable to attend class regularly, regardless of the reason or circumstance, should
withdraw from that class before poor attendance interferes with the student’s ability to
achieve the objectives required in the course. Withdrawal from class can affect eligibility
for federal financial aid.
IX.
Statement on Discrimination/Harassment
NACC and the Alabama State Board of Education are committed to providing both
employment and educational environments free of harassment or discrimination related to
an individual’s race, color, gender, religion, national origin, age, or disability. Such
harassment is a violation of State Board of Education policy. Any practice or behavior
that constitutes harassment or discrimination will not be tolerated.
X.
Statement of Adherence to ADA Guidelines
The Rehabilitation Act of 1973 (Section 504) and the Americans with Disabilities Act of
1990 state that qualified students with disabilities who meet the essential functions and
academic requirements are entitled to reasonable accommodations. It is the student’s
responsibility to provide appropriate disability documentation to the College.