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Aristotle (384 –322 BC) • Proposed the theory of spontaneous generation • Also called abiogenesis • Idea that living things can arise from nonliving matter • Idea lasted almost 2000 years Spontaneous Generation • For centuries, people based their beliefs on their interpretations of what they saw going on in the world around them without testing their ideas • They didn’t use the scientific method to arrive at answers to their questions • Their conclusions were based on untested observations “Evidence” for Spontaneous Generation Example 1 • Observation: Every year in the spring, the Nile River flooded areas of Egypt along the river, leaving behind nutrient-rich mud that enabled the people to grow that year’s crop of food. However, along with the muddy soil, large numbers of frogs appeared that weren’t around in drier times • Conclusion: It was perfectly obvious to people back then that muddy soil gave rise to the frogs Example 2 • Observation: In the cities centuries ago, there were no sewers, no garbage trucks, no electricity, and no refrigeration. Sewage flowed down the streets, and chamber pots and left over food were thrown out into the streets each morning. Many cities also had major rat problems and a disease called Bubonic plague. • Conclusion: Obviously, all the sewage and garbage turned into the rats. Example Observation: Since there were no refrigerators, the mandatory, daily trip to the butcher shop, especially in summer, meant battling the flies around the carcasses. Typically, carcasses were “hung by their heels,” and customers selected which chunk the butcher would carve off for them. • Conclusion: Obviously, the rotting meat that had been hanging in the sun all day was the source of the flies. Meat & Maggots He boiled broth in the flask to sterilize the broth. He boiled it much longer than Needham had done. While the broth was still very hot he sealed the flask. No bacteria grew in the sterilized broth. Critics said that boiling it too long had destroyed the invisible “life source” in the broth. They said that cool fresh air was needed to supply the necessary magic life force. 1862 - French Scientist Louis Pasteur was determined to prove that even microscopic life forms came from others of their own kind. He was certain that even bacteria came only from other bacteria of the same kind. Previous experiments had shown him that there were bacteria floating in the air. But how could he let air into a flask without also allowing bacteria to enter? First , he put broth into several flasks. He boiled the broth to sterilize the broth and the flasks. With a flame he heated the neck of the flasks to soften the glass. He pulled the softened glass into a long swan neck shape. Then he let the broth and flask cool. Although the bacteria are very small, they still have mass. He thought they would collect in the bottom curve of the neck, and air would move too slowly up the neck to carry the bacteria into the broth. No bacteria grew in the broth. They were trapped here. Then , he broke the neck off several of the flasks. Now bacteria in the air could enter the flasks! Bacteria grew in the broth. He had proven that all living things come from their own kind and not from spontaneous generation! Hooray!