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+ History of Life Chapter 14 Aristotle (384 –322 BC) •Proposed the theory of spontaneous generation •Also called abiogenesis •Idea that living things can arise from nonliving matter •Idea years + lasted almost 2000 + Spontaneous Generation For centuries, people based their beliefs on their interpretations of what they saw going on in the world around them without testing their ideas They didn’t use the scientific method to arrive at answers to their questions Their conclusions were based on untested observations + Example #1 Observation: Every year in the spring, the Nile River flooded areas of Egypt along the river, leaving behind nutrient-rich mud that enabled the people to grow that year’s crop of food. However, along with the muddy soil, large numbers of frogs appeared that weren’t around in drier times Conclusion: muddy soil gave rise to the frogs + Example #2 Observation: Since there were no refrigerators, the mandatory, daily trip to the butcher shop, especially in summer, meant battling the flies around the carcasses. Typically, carcasses were “hung by their heels,” and customers selected which chunk the butcher would carve off for them. Conclusion: the rotting meat that had been hanging in the sun all day was the source of the flies. + Spontaneous Generation Recipes Recipe for bees: Kill a young bull, and bury it in an upright position so that its horns protrude from the ground. After a month, a swarm of bees will fly out of the corpse. + Disproving Spontaneous Generation + Francesco Redi (1668) In 1668, Francesco Redi, an Italian physician, did an experiment with flies and wide-mouth jars containing meat His hypothesis was that rotten meat does not turn into flies Redi’s Experiment One Jar contained meat exposed to both air and flies Results: Found maggots that hatched into flies A second jar contained meat in a container wrapped in a fine cloth so that the meat was exposed to air, but not flies Results: No flies A third jar was sealed and thus not exposed to either air or flies Results: No flies + Results of Redi’s Experiments The results of this experiment disproved the idea of spontaneous generation for larger organisms People still thought microscopic organisms like algae or bacteria could arise that way. ****Wide-mouth jars each containing a piece of meat were subjected to several variations of “openness” while all other variables were kept the same. + Disproving Spontaneous Generation of Microbes + Lazzaro Spallanzani (1765) Conducted an experiment where he boiled soups for almost an hour and sealed containers by melting the slender necks closed. His hypothesis was that microorganisms formed not from the air but from other microorganisms + Spallanzani’s experiment Experimental group – Boiled broth for several hours to kill all the microorganisms in the broth and inside of the container Then: melted the necks of the containers shut while broth still hot •Broth would remain microbe free. However, when the flask was opened growth would occur Control group – Boiled broth for several hours to kill all the microorganisms in the broth and inside of the container •Broth became contaminated and cloudy + Results of Spallanzani’s experiment Conclusion: Boiled broth became contaminated only when microorganisms from the air entered the flask Critics said Spallanzani heated the flasks too long, destroying the “vital force” found in the air inside them. Air lacking “vital force”, they claimed, could not generate life. Therefore, spontaneous generation was kept alive for another century + Warm Broth is boiled 1. up question 1 yr passes, broth remains clear Flask is opened One day passes, broth becomes cloudy What does the 1st sealed flask contain? 2.How does the broth change during the experiment? + Pasteur Louis Pasteur (1822-1895) Hypothesis: put broth into several special S-shaped flasks Microbes come from cells of organisms on dust particles in the air; not the air itself. + Pasteur’s Experiment Pasture filled beakers with broth and curved the neck to create S-shaped Flasks that trapped any dust particles containing bacteria After boiling the broth to kill any microbes present the broth remained uncontaminated indefinitely If the neck was broken the broth became cloudy and contaminated with a day + The Theory of Biogenesis Pasteur’s S-shaped flask kept microbes out but let air in. Proved microbes only come from other microbes (life from life) - biogenesis Activity- Earth’s timeline Materials: 1 ruler, 1 sheet of paper, colored pencils or markers Instructions: Draw a horizontal line that will represent the timeline of Earth’s History The far left side starts at the current estimated formation of the Earth, mark 4.6 Billion Years ago The far right side represents present day Place these key events where your group estimates they happened and include a date, ex 2.6Bya, 100Mya, 14,000 yrs 1. 1st flowering plants 2. 1st Life 3. Multicellular organisms 4. Invasion of Land 5. 1st Eukaryotes 6. 1st Photosynthetic bacteria 7. Extinction of Dinosaurs 8. Last of the Glaciers recede from temperate zones 9. Homo Sapiens appeared 10. Continents appear essentially as they do today 3.8 Bya 1st Life 4By a 800 Mya Multicellular Organisms 250 Mya 1st flowering 20 Mya Plants Continents appear 15,000 Yrs do today as they Last Glaciers Recede 3 Bya 1st Photosynthetic bacteria 3By a 2By a 2.1 Bya 1st Aerobic Bacteria 4.6 Bya Formation of Earth 1.6 Bya 1st Eukaryotes 1By a 160,000 yrs Homo sapiens 65 Mya Extinction of Dinos 300 Mya Invasion of +