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Express Technology Co., Ltd.
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酶类产品系列：
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1、 醛糖还原酶
8、胆固醇酯酶
2、 辅羧酶脱羧辅酶
9、胰肽酶 E(胰弹性蛋白酶)
3、 V8 蛋白酶
10、山梨醇脱氢酶
4、 肽链内切酶
11、胆碱脂酶
5、 酪胺酸酶
12、β葡萄糖苷酶
6、 泛素活化酶 E1
13、乙酰辅酶 A 合成酶
7、 胶原酶
14、乙酰辅酶 A 氧化酶
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Aldose Reductase, recombinant
规格:0.4UNITS
Summary
This product is for research use only. Do not administer it to human.
Origin: Human muscle cell
Specific activity: 1.4 - 1.6 units/mg
Concentration: 1 units/mL
MW: approx. 36,000
Preparation: The enzyme is dissolved in 5 mM DTT, 50% glycerin and 50 mM Sodium phosphate
buffer solution (pH 7.0).
Aldose Reductase is purified from the culture medium containing baculovirus-insect cell line 32d gene
expression system. cDNA containing the entire protein coding region is expressed, and the enzyme
exhibits the same properties and characteristics of human muscle and retina.
Aldose Reductase catalyzes the reduction of glucose to sorbitol.
Sorbitol is subsequently converted to fructose by sorbitol dehydrogenase.
These two enzymes are key components of the polyol pathway, the alternate route of glucose
metabolism. It is suggested that aldose reductase is involved in the pathogenesis of diabetic
complications and the inhibitors continue to be developed at a vigorous pace.
[Reference]
Nishimura, C.
171 (1991).
et al.: Biochim Biophys. Acta., 1078,
Cocarboxylase tetrahydrate
Synonyms
Thiaminium diphosphoric acid ester tetrahydrate
Formula Hill
C12H18N4O7P2S * 4 H2O
At a glance
HS Code
2936 22 00
EC number
205-230-7
Molar mass
496.37 g/mol
WGK
1 (slightly polluting
substance)
Storage class (VCI)
10-13 Other liquids and
solids
CAS number
68684-55-9
Size
Ordering number
Package
5.00111.5000
Plastic bottle
5 kg
Formula Hill
C12H18N4O7P2S * 4 H2O
Solubility in water
Melting point
220 - 225 ºC (decomposition) Molar mass
pH value
2.4 - 3.2 (100 g/l, H2O,
20 ºC)
Chemical and physical data
55 g/l (25 ºC)
496.37 g/mol
Safety information
WGK
1 (slightly polluting substance)
Storage and Transport
HS Code
2936 22 00
WGK
1 (slightly polluting
substance)
Storage class (VCI)
10-13 Other liquids and
solids
Specification
Assay (HPLC, calc.on anhydrous substance)
96.0 - 101.0 %
Identity (IR-spectrum)
passes test
pH-value (10 %; water)
2.4 - 3.2
Chloride (Cl)
 0.01 %
Free phosphate (PO4)
 1.0 %
Heavy metals (as Pb)
 0.001 %
As (Arsenic)
 0.0003 %
Thiaminmonophosphoricacidester (HPLC)
 1.5 %
Thiamin (HPLC)
 0.1 %
Related substances (HPLC)
 1.5 %
Water
14.0 - 16.5 %
Endotoxins
< 12 I.U./g
Residual solvents (Ph. Eur./USP/ICH) class 3
 0.2 %
Other residual solvents (Ph. Eur./USP/ICH)
excluded by
manufacturing process
Cocarboxylase (chloride)
Synonyms
Thiaminium diphosphoric acid ester chloride, Thiaminium pyrophosphate
chloride, Thiaminium pyrophosphoric acid ester chloride
Formula Hill
C12H19ClN4O7P2S
At a glance
HS Code
2936 22 00
EC number
205-836-1
Molar mass
460.77 g/mol
WGK
1 (slightly polluting
substance)
Storage class (VCI)
10-13 Other liquids and
solids
CAS number
154-87-0
Size
Ordering number
Package
5.00112.1000
Plastic bottle
1 kg
5.00112.5000
Plastic bottle
5 kg
Chemical and physical data
Formula Hill
C12H19ClN4O7P2S
Solubility in water
Melting point
240 - 244 ºC (decomposition) Molar mass
pH value
1.0 - 1.4 (100 g/l, H2O,
20 ºC)
220 g/l (20 ºC)
460.77 g/mol
Safety information
WGK
1 (slightly polluting substance)
Storage and Transport
HS Code
2936 22 00
WGK
1 (slightly polluting
substance)
Storage class (VCI)
10-13 Other liquids and
solids
Specification
Assay (HPLC, calc. on dried substance)
 93.0 %
Identity (IR-spectrum)
passes test
Appearance of solution (10 %; water)
passes test
pH-value (10 %; water)
1.0 - 1.4
Chloride (Cl) (calculated on dried substance)
7.5 - 7.9 %
Free phosphate (PO4)
 1.0 %
Sulphate (SO4)
 0.01 %
Heavy metals (as Pb)
 0.001 %
As (Arsenic)
 0.0003 %
Thiamin Monophosphoric Acid Ester Chloride (HPLC)
 4.0 %
Thiamin Dichloride (HPLC)
 0.5 %
Related substances (HPLC)
 1.5 %
Loss on drying (130 ºC)
 1.5 %
Endotoxins
 12 I.U./g
Microbiological test
passes test
Methanol (GC)
 2.0 %
Other residual solvents (Ph. Eur./USP/ICH)
excluded by
manufacturing process
Assay (HPLC, calc. on dried substance)
 94.5 %
Identity (IR-spectrum)
passes test
Appearance of solution (10 %; water)
passes test
pH-value (10 %; water)
1.0 - 1.4
Chloride (Cl) (calculated on dried substance)
7.5 - 7.9 %
Free phosphate (PO4)
 1.0 %
Sulphate (SO4)
 0.01 %
Heavy metals (as Pb)
 0.001 %
As (Arsenic)
 0.0003 %
Thiamin Monophosphoric Acid Ester Chloride (HPLC)
 4.0 %
Thiamin Dichloride (HPLC)
 0.5 %
Related substances (HPLC)
 1.5 %
Loss on drying (130 ºC)
 1.5 %
Endotoxins
 12 I.U./g
Microbiological test
passes test
Methanol (GC)
 2.0 %
Other residual solvents (Ph. Eur./USP/ICH)
excluded by
manufacturing process
correspons JSPI
Endoproteinase Glu-C Staphylococcus aureus V8
BioChemika lyophilized powder color light brown 400 units/mg
Identifiers
Synonyms
CAS Number
Enzyme
Commission (EC)
V8 Protease
66676-43-5
3.4.21.19
Number
EG/EC Number
MDL number
2326424
MFCD01324998
Description
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1µmol N-tBoc-L-glutamic
acid--phenyl ester per minute at pH 7.8 and 37°C
Properties
mol wt
storage temp.
Mr~11400
20°C
Safety Information
Hazard Codes
Xn
Risk Statements
36/37/38-42/43
Safety Statements 22-24-26-36/37
Lysyl Endopeptidase(R)
规格: 1 vial (2 AU) , 1 vial (10 AU)
Grade: for Biochemistry
Summary
This product is for research use only. Do not administer it to human.
Purity: Homogenous on SDS-PAGE
Activity: shown on each label. Unit: AU*/vial
* AU: Amidase Unit - amount of enzyme required for the formation of 1 umol of p-nitroaniline
from
N-benzoyl-DL-lysine-p-nitroaniline per minute at 30 degrees C, pH 9.5.
Preparation A 10AU-vial contains Tris-HCl corresponding to 1 mL of 2 mM Tris HCl buffer (pH
8.0).
Molecular Weight] 27,000 (gel filtration); 30,000 (SDS electrophoresis)
Optimal pH 9.0 -9.5 (amidase activity)
Stability Very stable in lyophilized forms at -20 degrees C; Stable for at least 2 years at -20
degrees C
when dissolved in Tris-HCl buffer at pH 4.0 to 11 at the enzyme concentration of more than 1
mg/mL. Stable for at
least 24 hours at 4 degrees C under the above conditions.
Lysyl Endopeptidase is a protease secreted by the soil bacterium Achromobacter lyticus
M497-1,
and is synonymous with the achromobacter protease discovered by Masaki, et al. Lysyl
Endopeptidase
cleaves peptide bonds at the carboxy-terminal of both lysine residues and
S-aminoethylcysteine residues with a
high degree of specificity, making it a valuable tool for analysis of peptide and protein primary
structure and for
enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its
ability to retain complete
activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.
Tyrosinase from mushroom
BioChemika, lyophilized, ≥2000 units/mg
Descriptions
Other Notes Kinetic study of the suicide inactivation of tyrosinase1 ; Chromogenic assay
Unit Definition 1 U corresponds to the amount of enzyme which increases the absorbance
at 305 nm by 0.001 per minute at pH 6.5 and 25°C (L-tyrosine as substrate, 3.0 mL reaction
mix); 30 absorbance-U as described above are equivalent to ~1 U [when 1 U is the amount of
enzyme which oxidyzes 1 μmol 4-methylcatechol per minute at pH 6.5 and 25°C]
General description Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering
measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It
is responsible for browning reactions. The enzyme is reported to have two binding sites for
aromatic substrates and a different binding site for oxygen-copper. The copper is probably
Cu(I), with inactivation involving oxidation to Cu(II) ion.
Properties
mol wt
Mr ~125000
solubility
0.02 M phosphate, pH 6.5: 5mg/mL, clear, slightly brown to deep brown
storage temp.
−20°C
Ubiquitin Activating Enzyme (E1) Rabbit
规格：25ug
Descriptions
Application
Enzyme responsible for the first step in ubiquitin-protein isopeptide bond formation. E1
catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a
high-energy thioester bond in an ATP-dependent manner.
Other Notes
manufactured for Sigma by Boston Biochem. Inc.
Physical form
Solution in 50 mM HEPES, pH 7.6
Properties
mol wt
mol wt ~110 kDa
Shipped in
dry ice
storage temp.
−70°C
Collagenase
规格：25MG
贮存：2-10C
Grade: for Collagen Analysis
Summary
This product is for research use only. Do not administer it to human.
Activity: min. 1,000 Mandle units/mg
Optimum pH: 7.0-8.0
This Collagenase is used for the identification and quantitative analysis of collagen and is also
used as a debriding agent.
Appearance: White ¡Á nearly white, crystals ¡Á crystalline powder
Synonym COLLAGENASE, CLOSTRIDIUM HISTOLYTICUM
Collagenase
Clostridiopeptidase A
CAS NO. 9001-12-1
EC NO. 3.4.24.3
Molecular Formula Molecular Weight about 180,000
Cholesterol Esterase
规格：500U、1000U
贮存：2-10C
Grade: for Biochemistry
Summary
This product is for research use only. Do not administer it to human.
Appearance: Nearly white ¡Á pale brown, crystals ¡Á powder or small mass
Synonym Cholesterol Esterase
Esterase, cholesterol
Sterol-ester Hydrolase
CE
CAS NO. 9026-00-0
EC NO. 3.1.1.13
Molecular Weight 129,000
Elastase, from Porcine Pancreas
规格：20MG
贮存：2-10C
Appearance: White - slightly brown, crystalline powder - powder
Activity: min.130units/mg
Synonym Elastase (pig pancreas)
Elastase, Porcine
Elastase, pancreatic
Elastase, Pancreatic Pancreatopeptidase E
Elastase
CAS NO. 39445-21-1
Molecular Weight 25,900
sorbitol Dehydrogenase, from Microorganism
规格：50 units
贮存：-20C
Grade: for Biochemistry
Summary
This product is for research use only. Do not administer it to human.
For Research on Diabetic Complications & For Accurate Quantification of Sorbitol
Sorbitol Dehydrogenase is an essential enzyme involved in polyol metabolism. Sorbitol
Dehydrogenase, isolated from microorganisms, is characterized by its high substrate
specificity and it degrades D-sorbitol, used as a substrate, into fructose. It is believed that
diabetic complications are caused by sorbitol accumulation. The conventional enzymes on
the market react with sugars other than sorbitol; however, Sorbitol Dehydrogenase with high
substrate specificity enables accurate quantification of sorbitol.
When reacted with typical sugars as substrates including glucose, mannitol, and galactose (at
0.5 mol/L concentration) independently or in a mixture, the absorbance did not change,
proving high substrate specificity of Sorbitol Dehydrogenase. Sorbitol Dehydrogenase is
useful not only in research aggravation mechanism of complications but also in sorbitol
quantification in foods.
Activity: 40 - 60units/vial
Cholinesterase, from Horse Serum
Grade: for Biochemistry
Storage condition: Keep at -20 degrees C.
Pkg. Size:1,000 Units
Summary
This product is for research use only. Do not administer it to human.
Specific activity: 3-6 units/mg
Activity: min. 10 units/mg
Molecular Weight: 300,000
Optimum pH: approx. 8.0-8.5
Isoelectric point: 2.9-3.0, 4.36
Preparation: Lyophilized powder
Appearance: White ¡Á grayish brown, powder
Synonym
Butyrylcholine Esterase
Cholinesterase
Esterase, choline
Choline Esterase
Choline Esterase, Butyryl
Butyryl Cholinesterase
Acylcholine Acyl-hydrolase
Cholinesterase, Butyryl-
Pseudocholineesterase
CAS NO. 9001-08-5
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beta-Glucosidase, from Almond
Storage condition: Keep at -20 degrees C.
Pkg. Size:50000un ,10000UN , 2000UNITS
Summary
This product is for research use only. Do not administer it to human.
Synonym beta-Glucosidase
beta-D-Glucosidase
Linamarase
Glucosidase, .beta.Glucosidase, betabeta-D-glucoside Glucohydrolase
Amygdalase
b-Glucosidase
Cellobiase
CAS NO. 9001-22-3
Acyl-coenzyme A Synthetase from Pseudomonas sp.
BioChemika, lyophilized, powder, white, ≥0.6 units/mg
规格:1mg
Synonym Acid: CoA ligase (AMP-forming)
CAS Number9013-18-7
Descriptions
Other Notes For the determination of free fatty acids in serum1
Unit Definition 1 U corresponds to the amount of enzyme which forms 1 μmol AMP and
oleoyl-CoA from ATP and oleate per minute at pH 8.1 and 25°C, in the presence of CoA
Shipped in wet ice
storage temp. −20°C
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Acyl Coenzyme A Oxidase from Arthrobacter sp.
lyophilized powder, 10-40 units/mg protein
规格:25UN , 50UN
Descriptions
Unit Definition One unit will form 1.0 μmole of H2O2 and hexadecenoyl-CoA from
palmitoyl-CoA per min at pH 8.0 at 30 °C in a peroxidase coupled system.
Physical form Contains flavin adenine dinucleotide as stabilizer; protein determined by
biuret
storage temp. −20°C
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伊普瑞斯科技有限公司
www.express-cn.com
[email protected]
[email protected]
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Fax:010-60206773
联系人：Colin Kan(阚先生)