Download 2010_3_zinojums - Latvijas biomedicīnas pētījumu un studiju

APP Latvijas Biomedicīnas pētījumu un studiju centra
Iepirkumu komisijas
Z I Ņ O J U M S Nr. 24
Rīga
2010. gada 23. jūlijā
1. Iepirkuma identifikācijas numurs: BMC2010/3_ESF
2. Ievietots internetā: 27.05.2010
3. Valsts pasūtītājs: APP Latvijas Biomedicīnas pētījumu un studiju centrs, adrese:
Rātsupītes1, Rīga LV 1067, Latvija, PVN LV90002120158, Tālrunis (371) 67808216,
Telefakss (371) 67442407, E-mail: [email protected]
4. Valsts pasūtījuma priekšmets:
Materiālu un molekulārās bioloģijas reaģentu piegāde. Paredzēts noslēgt vispārējo
vienošanos par atbilstošo preču piegādi uz 30 mēnešiem no vienošanās noslēgšanas brīža.
Preču kopējā vērtība: līdz 87887,99 latu periodā.
5. Valsts pasūtījuma piešķiršanas metode – Atklāts konkurss.
Metodes izvēles pamatojums: Valsts pasūtījuma raksturs un kopējā vērtība atbilst " Publisko
iepirkumu likuma" un tam pakārtoto informatīvo aktu nosacījumiem.
6. Prasības pretendentiem:
6.1. Reģistrācijas apliecības kopiju vai izziņas, kas apliecina, ka pretendents ir reģistrēts
likumā noteiktajā kārtībā.
6.2. Rakstisks apliecinājums, ka uz pretendentu neattiecas no Publisko iepirkumu likuma
39.panta pirmajā daļā minētajiem nosacījumiem (ņemot vērā šā panta trešajā daļā minētos
termiņus). Konkursa uzvarētājiem ir jābūt gataviem iesniegt informāciju atbilstoši Publisko
iepirkumu likuma 39. panta sestās daļas regulējumam.
6.3. Ja pasūtītājam ir radušās šaubas par pretendenta reputāciju, tas ir tiesīgs no pretendenta
papildus pieprasīt nepieciešamo informāciju par tā komercdarbību likumā paredzētajā
kārtībā.
7. Vērtēšanas kritēriji:
Piedāvājums ar viszemāko cenu.
8. Piedāvājumu iesniegšanas vieta un termiņš:
Piedāvājumi iesniedzami līdz 2010. g. 1. jūlijam APP Latvijas Biomedicīnas pētījumu un
studiju centrā Rātsupītes ielā 1, Rīgā, sekretariātā Svetlanai Kazmerikai darba dienās no 1000
līdz 1500. Piedāvājuma derīguma laiks tiek noteikts 60 kalendārās dienas no piedāvājuma
atvēršanas brīža.
9. Piedāvājumu atvēršanas vieta un laiks: APP Latvijas Biomedicīnas pētījumu un studiju
centrs, 2010.g. 1. jūlijs plkst. 1500.
10. Piedāvājuma atvēršanā klātesošās personas:
1
Iepirkumu komisijas locekļi, kā arī sekojošie pretendentu pārstāvji (attiecīgi sarakstam
pielikumā):
Aleksandrs Šepteličs, Interlux SIA
Sergejs Andrejevs, Interlux SIA
2
11. Pretendentu iesniegto preču cenu saraksts:
1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti).
npk
Viela
1
2
Rubidium chloride
Tryptone, 1 kg
3
o-Phenylenediamine dihydrochloride
tablet, 5 mg substrate per tablet
4
cycloheximide
5
Minisart-Plus filters 0.45µm, 50/pack
6
7
8
9
Calcium chloride hexahydrate
Copper(II) sulfate pentahydrate, puriss.
p.a., ACS reagent, reag. ISO, reag. Ph.
Eur., 99-102%
Diethyl pyrocarbonate
Sigma
83979
T9410
P6912-100TAB
01810-5G
17829K
21108-500G
31293-1KG
40718-25ML
10
MES monohydrate
Sodium chloride, ≥99.5%, BioUltra, for
molecular biology, 5kg
69892-100G
11
12
13
SDS
PEG 1000
PEG 200
71729-100G
81188-250G
88440-250ML-F
14
PEG 300
90878-250ML-F
15
16
17
18
19
PEG 400
Trizma base
PEG 4000
Agarose
Anti-trušu antivielas peroksidāzes konjugāts
91893-250ML-F
93349-1KG
95904-250G-F
A9539-500G
A0545-1ML
71376-5KG
Ekvivalents produkts, daudzums, ražotājs,
produkta kods (Interlux)
Rubidium chloride, 25g, Amresco, 0632-25
Tryptone, 1 kg, BD, 211705
o-Phenylenediamine dihydrochloride
tablet, 5 mg substrate per tablet, 100 tab,
Sigma, P6912-100TAB
Cycloheximide, 5g, AppliChem Lifescience,
A0879.0005
Minisart-Plus filters 0.45µm, 50/pack,
Sartorius, 17829K
Calcium chloride hexahydrate, 500g,
AppliChem Lifescience, A2303.1000
Copper(II) sulfate pentahydrate, pure. p.a.,
reag. Ph. Eur., 99-101%, 1kg, AppliChem
Lifescience, A1367.1000
Diethyl pyrocarbonate, 25ml, Molekula,
11155018
MES monohydrate, 100g, AppliChem
Lifescience, A1074.0100
Sodium chloride, ≥99.5%, for molecular
biology, 5kg, BDH Prolabo, 443827W
SDS, 100g, AppliChem Lifescience,
A1112.0100
PEG 1000, 250g, Alfa Aesar, B22134
PEG 200, 250ml, Alfa Aesar, B21918
PEG 300, 250ml, AppliChem Lifescience,
A5537.0250
PEG 400, 250ml, AppliChem Lifescience,
A2203.0500
Trizma base, 1kg, BDH Prolabo, 28808.294
PEG 4000, 250g, Alfa Aesar, A16151
Agarose, 500g, StarLab GmbH, N3101-0500
Anti-trušu antivielas peroksidāzes konjugāts,
Interlux
Labochema
69,40
86,20
99,26
161,46
260,00
200,46
104,05
95,58
77,50
73,00
18,80
20,99
23,00
40,77
54,70
69,92
31,90
55,77
29,60
68,80
26,20
14,40
14,00
31,84
13,24
10,73
14,20
14,03
13,60
36,30
17,00
164,00
179,09
12,22
132,15
11,01
415,82
128,13
3
20
Agar
A1296-100G
21
G-418 disulfate salt, 5g
22
23
Aluminium oxide Type A-5
Amicase, 1kg
A2039-500G
A2427
24
25
26
Acrylamide/Bis-acrylamide
L-(+)-Arabinose
L-(+)-Arabinose, 500g
A2729-1L
A3256-100G
A3256-500G
27
Anti-his antivielas peroksidāzes konjugāts
A1720-5G
A7058-1VL
31
Monoclonal anti-polyHistidine-Peroxidase
antibody from mouse
BSA; pH ~7 (1% in 0.15 M NaCl), ≥98%
(agarose gel electrophoresis), lyophilized
powder (Sigma-Aldrich)
Anti-Rabbit IgG (whole molecule)–
Peroxidase antibody produced in goat. IgG
fraction of antiserum, buffered aqueous
solution, 1 ml
Anti-Mouse IgG (whole molecule)–
Peroxidase antibody produced in rabbit. IgG
fraction of antiserum, buffered aqueous
solution, 2 ml
32
Anti-Rabbit IgG (whole molecule)–
Peroxidase antibody produced in goat
A9169-2ML
33
Brilliant Blue R
B7920-50G
34
SIGMAFAST BCIP/NTB, 25 tabletes
35
Boric acid, 1kg
B6768-1KG
36
Chloramphenicol, 100g
C0378-100G
28
29
30
A7058-1VL
A7906-100G
A8275 - 1ML
A9044 - 2ML
B5655-25TAB
1mg, Invitrogen, G-21234
Agar, 100g, BD, 214050
G-418 disulfate salt, 5g, AppliChem
Lifescience, A2167.0001
Aluminium oxide Type A-5, 500g, Sigma,
A2039-500G
Amicase, 1kg, Sigma, A2427-1KG
Acrylamide/Bis-acrylamide, 1L, AppliChem
Lifescience, A3658.1000
L-(+)-Arabinose, 100g, Molekula, 30957927
L-(+)-Arabinose, 500g, Molekula, 30957927
Anti-his (C-term)antivielas peroksidāzes
konjugāts, 1VL, 50 µl, Invitrogen, R930-25
Monoclonal anti-polyHistidine-Peroxidase
antibody from mouse,50 µl, Invitrogen, R93025
BSA; pH ~7, ≥98% (agarose gel
electrophoresis), lyophilized powder, 100g,
AppliChem Lifescience, A1391.0100
Anti-Rabbit IgG (whole molecule)–
Peroxidase antibody produced in goat. IgG
fraction of antiserum, buffered aqueous
solution, 1mg, Invitrogen, G-21234
Anti-Mouse IgG (whole molecule)–
Peroxidase antibody produced in goat. IgG
fraction of antiserum, buffered aqueous
solution, 1mg, Invitrogen, G-21040
Anti-Rabbit IgG (whole molecule)–
Peroxidase antibody produced in goat, 1mg,
Invitrogen, G-21234
Brilliant Blue R, 50g, AppliChem Lifescience,
A1092.0025
SIGMAFAST BCIP/NTB, 25 tabletes, Sigma,
B5655-25TAB
Boric acid, 1kg, AppliChem Lifescience,
A2940.1000
Chloramphenicol, 100g, AppliChem
Lifescience, A7495.0100
54,90
43,57
320,40
322,28
34,72
112,45
39,77
128,67
77,18
77,70
388,60
173,05
71,79
287,75
214,73
258,43
214,73
258,43
100,00
124,46
179,09
75,12
172,09
114,43
179,09
135,66
48,00
53,20
143,00
128,38
24,80
31,33
42,20
58,96
4
37
38
Carbenicillin, disodium salt, 89.0-100.5%
anhydrous basis
CelLytic™ M
39
Cesium chloride
40
41
42
Chamber slides, sterile, 4 wells on glass
NZ-Amine A, 1 kg
DMSO
43
DMEM- high glucose
44
EDTA, anhydrous, ≥99% (titration)
EDS-1KG
45
EDS-500G
48
EDTA, 500g
Fibronectin solution from human fibroblasts,
100 UL
Fibronectin from human plasma cell culture
tested, lyophilized powder, 1mg
Anti-Fibronectin antibody produced in
rabbit. affinity isolated antibody, buffered
aqueous solution, 0.2ml
49
Freund`s Adjuvant, incomplete
F5506-6X10ML
50
Freund`s Adjuvant, complete
F5881-6X10ML
51
Formamide
F7503-500ML
52
F7524-500ML
53
Fetal Bovine Serum
Formaldehyde (37%) Solution (Formalin),
500 ml
54
L-Glutathione reduced
G4251-25G
55
D-(+)-galactose
L-Glutamic acid potassium salt
monohydrate
G0625-100G
46
47
56
C1389-1G
C2978-250ML
C3139-500G
C6807-1PAK
C7290
D2650-100ML
D6429-6X500ML
F0556-100UL
F2006-1MG
F3648-.2ML
F8775-500ML
G1501-500G
Carbenicillin, disodium salt, 89.0-100.5%
anhydrous basis, 1g, AppliChem Lifescience,
A1491.0001
CelLytic™ M, 250ml, Sigma, C2978-250ML
Cesium chloride, 500g, BDH Prolabo,
441574M
Chamber slides, sterile, 4 wells on glass,
16gab./iepak., Nunc, 734-2049
NZ-Amine A, 1 kg, Amresco, EJ853
DMSO, 100ml, Sigma, D2650-100ML
DMEM- high glucose, 6x500ml, Invitrogen,
31966021
EDTA, anhydrous, ≥99%, 1kg, AppliChem
Lifescience, A5097.1000
EDTA, 500g, AppliChem Lifescience,
A1103.0500
Fibronectin solution from human fibroblasts,
100 UL, Sigma, F0556-100UL
Fibronectin Natural Human, 1 mg. From
human plasma, Invitrogen, PHE0023
Anti-Fibronectin antibody produced in rabbit.
affinity isolated antibody, buffered aqueous
solution, 0.2ml, Sigma, F3648-.2ML
Freund`s Adjuvant, incomplete, 6x10ml,
Sigma, F5506-6X10ML
Freund`s Adjuvant, complete, 6x10ml,
Sigma, F5881-6X10ML
Formamide, 500ml, AppliChem Lifescience,
A0937.0500
Fetal Bovine Serum, 500ml, Invitrogen,
10106169
Formaldehyde (37%) Solution (Formalin),
500 ml, AppliChem Lifescience, A0877.0500
L-Glutathione reduced, 25g, AppliChem
Lifescience, A2084.0025
D-(+)-galactose, 100g, AppliChem
Lifescience, A1131.0100
L-Glutamic acid potassium salt monohydrate,
500g, Alfa Aesar, A17232
21,90
145,00
94,95
137,37
377,40
601,02
97,82
99,00
115,09
133,92
82,03
101,65
66,00
21,60
43,50
72,53
20,69
35,52
131,00
124,36
182,39
133,90
60,00
45,30
103,00
77,60
102,00
77,23
13,70
18,27
75,00
79,65a)
29,00
40,99
74,20
103,66
19,00
23,10
55,30
114,38
5
57
Gold(III) chloride trihydrate, 1g
G4022-1G
58
Sephadex® G-50, Superfine
G5050-50G
59
D-(+)-Glucose, ≥99% (GC)
G5400-250G
60
61
Glutamine solution
Hepes
62
Anti-polyHistidine antibody, monoclonal,
produced in mouse, 0.2 ml
63
HEPES
G7513-100ML
H0887-100ML
H1029-.2ML
H3375-250G
64
Iscove's modified dulbecco's medium
65
Imidazole
I0125-100G
66
67
Kanamycin sulfate, 50g
LB Broth
K4000-50G
L3022-1KG
68
69
70
Leucine
I3390-6X500ML
MOPS
MTT Formazan
L8000-250G
M1254-100G
M2003-1G
71
Nocodazole
M1404-2MG
72
mitomycin C
M4287-5X2MG
73
74
75
Magnesium acetate
M5661-250G
M9 salts 5X
M6030-250G
76
2-Mercaptoethanol
Mouse monoclonal anti-Nanog IgG1, Clone
NNG-811, 25 ul
77
78
Nutrient Mixture F-10 Ham
Nitrocellulose membrane for blotting,
M6250-250ML
N3038
N6013-6x500ML
N8267-5EA
Gold(III) chloride trihydrate, 1g, Alfa Aesar,
36400
Sephadex® G-50, Superfine, 50g, GE
Healthcare, 17-0041-01
D-(+)-Glucose, ≥99%, 250g, AppliChem
Lifescience, A1422.0500
Glutamine solution, 100ml, Invitrogen,
25030024
Hepes, 100ml, Invitrogen, 15630056
Anti-polyHistidine antibody, monoclonal,
produced in mouse, 50 µl, Invitrogen, R93025
HEPES, 250g, AppliChem Lifescience,
A3268.0250
Iscove's modified dulbecco's medium, 6 x
500ml, Invitrogen, 31980022
Imidazole, 100g, AppliChem Lifescience,
A1073.0100
Kanamycin sulfate, 50g, AppliChem
Lifescience, A1493.0050
LB Broth, 1kg, BD, 1231
Leucine, 250g, AppliChem Lifescience,
A3460.0250
MOPS, 100g, BDH Prolabo, 443832T
MTT Formazan, 1g, Sigma, M2003-1G
Nocodazole, 2mg, AppliChem Lifescience,
A1999.0010
Mitomycin C, 5x2mg, AppliChem Lifescience,
A2190.0002
Magnesium acetate, 250g, AppliChem
Lifescience, A1877.0250
M9 salts 5X, 250g, Sigma, M6030-250G
2-Mercaptoethanol, 250ml, BDH Prolabo,
436024C
Mouse monoclonal anti-Nanog IgG1, Clone
NNG-811, 25 ul, Sigma, N3038-25UL
Nutrient Mixture F-10 Ham, 6x500ml,
Biochrom, F0715
Nitrocellulose membrane for blotting,
75,40
95,20
292,50
132,88
13,60
18,75
8,00
43,00
14,11
34,14
214,73
229,07
76,70
104,93
108,00
100,15
15,10
26,71
144,50
42,90
399,95
63,57
49,00
26,20
76,00
63,18
59,48
57,44
54,10
20,01
204,50
297,14
19,90
50,37
30,97
46,15
14,00
19,66
52,50
49,74
38,70
76,40
41,67
74,54
6
0,45mkm, 15x15cm
79
Glass slides coated with poly-L-lysine
P0425-72EA
80
81
82
Potassium phosphate monobasic, 1kg
Pepticase, 250 g
Tween 20
P0662-1KG
P1192
P1379-100ML
83
Tween 20
P1379-1L
84
85
PEG 8000
PEG 3350
P2139-500g
P3640-500g
86
Phosphate buffered saline powder, pH 7.4
P3813-10PAK
87
Phosphate buffered saline
P4417-100TAB
88
Phosphate-Citrate Buffer
P4809-100TAB
89
Parafilm® "M"
roll size 2 in. × 250 ft
P7543-1EA
90
Protein A–Peroxidase from Staphylococcus
aureus/horseradish, 1 mg
P8651-1MG
91
RPMI 1640 Medium
92
Retinoic acid
R2625-50MG
93
R4875-100MG
95
Ribonuclease A Type I-A
Sucrose, for molecular biology, ≥99.5%
(GC), 5kg
Sodium citrate tribasic dihydrate, ACS
reagent, ≥99.0%
96
Thiamine hydrochloride
T4625-250G
97
Trypsin-EDTA solution
T4049-100ML
94
R0883-6x500ML
S0389-5KG
S4641-500G
0,45mkm, 15x15cm, 5gab., Whatman, 7320354
Glass slides coated with poly-L-lysine,
72gab., Sigma, P0425-72EA
Potassium phosphate monobasic, 1kg,
AppliChem Lifescience, A3620.1000
Pepticase, 250 g, Sigma, P1192-250G
Tween 20, 100ml, BDH Prolabo, 437082Q
Tween 20, 1L, AppliChem Lifescnience,
A4974.1000
PEG 8000, 500g, AppliChem
LifescienceA2204.0500
PEG 3350, 500g, Sigma, P3640-500G
Phosphate buffered saline powder, pH 7.4,
10pak, Sigma, P3813-10PAK
Phosphate buffered saline, 100tab, Amresco,
E404-100
Phosphate-Citrate Buffer, 100tab, Sigma,
P4809-100TAB
Parafilm® "M"
roll size 2 in. × 250 ft, 1gab., Brand, 2911214
Protein A–Peroxidase from Staphylococcus
aureus/horseradish, 1 mg, Sigma, P86511MG
RPMI 1640 Medium, 6x500ml, Invitrogen,
11835063
Retinoic acid, 50mg, AppliChem Lifescience,
A6947.0100
Ribonuclease A Type I-A, 100mg, AppliChem
Lifescience, A2760.0100
Sucrose, for molecular biology, ≥99.5% (GC),
5kg, AppliChem Lifescience, A2211.5000
Sodium citrate tribasic dihydrate, ACS
reagent, ≥99.0%, 500g, Alfa Aesar, A12274
Thiamine hydrochloride, 250g, AppliChem
Lifescience, A0955.0250
Trypsin-EDTA solution, 100ml, Sigma,
T4049-100ML
48,50
36,90
30,30
54,00
9,39
37,67
51,48
15,00
40,40
34,88
13,60
16,41
18,45
15,06
42,39
38,83
42,00b)
69,11
166,00
156,49
21,90
22,78
195,00
183,95
105
59,82
23,00
17,60
23,60
48,02
103,70
158,94
14,40c)
21,75
54,20
99,13
11,90
9,01
7
98
Trypsin proteomics grade
T6567-5X20UG
99
Tryptose Phosphate Broth 1x
T8159-100ML
100
101
102
103
Tryptone
TRI – Reagent
TRI Reagent, 200 ml
Yeast Extract, 1kg
T9410-250G
T9424-100ML
T9424-200ML
Y1625
104
Plastibrand PD-tips 1.25ml
Z332631-1PAK
105
Plastibrand PD-tips 2.5ml
Z332658-1PAK
106
Plastibrand PD-tips 5ml
Z332666-1PAK
107
Nalgene® vacuum filtration system capacity
150 mL, pore size 0.2 μm, 12 pcs.
Z358185-1CS
108
Carboy with spigot, polypropylene, 20l,
autoclavable
Z360260-1EA
112
Nalgene® vacuum filtration system capacity
250 mL, pore size 0.2 μm, 12 pcs.
Hanna pH standard buffer solution pH 4.01,
500ML
Hanna pH standard buffer solution pH 7.01,
500ML
Hanna pH standard buffer solution pH
10.01, 500ML
113
Digitonin
D141-500MG
114
EGTA
E3889-100G
115
HEPES
H4034-500G
116
117
Mitomycin C
Cycloheximide
109
110
111
Z370606-1CS
Z655112-500ML
Z655139-500ML
Z655155-500ML
M4287-5X2MG
01810-5G
Trypsin proteomics grade, 5x20ug, Sigma,
T6567-5X20UG
Tryptose Phosphate Broth 1x, 100ml, Sigma,
T8159-100ML
Tryptone, 250 g, BD, 211921
TRI – Reagent, 100ml, Sigma, T9424-100ML
TRI Reagent, 200 ml, Sigma, T9424-200ML
Yeast Extract, 1kg, BD,
Plastibrand PD-tips 1.25ml, 1pak(100gab.),
Brand, 612-5802
Plastibrand PD-tips 2.5ml, 1pak(100gab.),
Brand, 612-5803
Plastibrand PD-tips 5ml, 1pak(100gab.),
Brand, 612-5804
Nalgene® vacuum filtration system capacity
150 mL, pore size 0.2 μm, 12 pcs., Nunc
Nalgene, 514-0024
Carboy with spigot, polypropylene, 20l,
autoclavable, 1gab., Nunc Nalgene, 2168762
Nalgene® vacuum filtration system capacity
250 mL, pore size 0.2 μm, 12 pcs., Nunc
Nalgene, 514-0027
Hanna pH standard buffer solution pH 4.01,
500ML, Hanna, HI6004
Hanna pH standard buffer solution pH 7.01,
500ML, Hanna, HI6007
Hanna pH standard buffer solution pH 10.01,
500ML, Hanna, HI6010
Digitonin, 500mg, AppliChem Lifescience,
A1905.0500
EGTA, 100g, AppliChem Lifescience,
A0878.0100
HEPES, 500g, AppliChem Lifescience,
A3268.0500
Mitomycin C, 5x2mg, AppliChem Lifescience,
A2190.0002
Cycloheximide, 5g, AppliChem Lifescience,
74,00
55,82
15,00
10,52
55,00
177,00
320,00
96,00
57,40
143,23
245,18
266,93
41,40
40,55
41,40
40,55
41,40
40,55
74,40
63,10
67,80
98,47
83,40
70,80
31,50
21,24
31,50
21,24
31,50
21,24
99,60
125,19
163,30
185,01
132,90
167,53
204,50
104,00
297,14
102,94
8
A0879.0005
OptiPrep Density Gradient Medium, 250ml,
118 OptiPrep Density Gradient Medium
D1556-250ML
Sigma, D1556-250ML
216,74 198,43
109 MG-132
C2211-5MG
MG-132, 5mg, Sigma, C2211-5MG
112,00
84,85
120 Lactacystine
L6785-2MG
Lactacystine, 0,2mg, Cayman, 70980-0.5
313,40 225,35
Mitomycin C, 5x2mg, AppliChem Lifescience,
121 Mitomycin C
M4287-5X2MG
A2190.0002
204,50 297,14
ß-Galactosidase Reporter Gene Staning kit,
122 ß-Galactosidase Reporter Gene Staning kit
GALS-1KT
1kit, Sigma, GALS-1KT
199,04 219,65
X-Gal, 100mg, AppliChem Lifescience,
123 X-Gal
A4978.0100
18,00
B4252-100MG
29,30
Magnesium acetate tetrahydrate, 1kg,
124 Magnesium acetate tetrahydrate
M2545-1KG
AppliChem Lifescience, A1877.1000
50,20
133,89
Glycine (ReagentPlus®, ≥99%), 1kg,
125 Glycine (ReagentPlus®, ≥99% (TLC) grade)
G7126-1KG
AppliChem Lifescience, A1067.1000
27,40d)
39,19
a) Fetal Bovine Serum ir bioloģiskas izcelsmes substance un tā var atšķirties atkarībā no ražotāja. Tāpēc līgumu nolemts slēgt ar abiem piegādātājiem.
b) PBS Amresco piedāvā to pašu tablešu skaitu par zemāku cenu, taču viņu 1 tablete ir jāšķīdina 100ml, kamēr Sigmas - 200ml, reāli sanāk 2x mazāk PBSa un Sigmas ir lētāks;
c) Sodium citrate tribasic dihydrate, ACS reagent, =99.0%. Tika prasīts ACS grade, bet tas netiek piedāvāts (t.i. Alfa Aesar mājas lapā ir atrodams arī ACS grade nātrija citrāts (zem
cita koda), bet jau par pavisam citu cenu);
d) Glycine (ReagentPlus®, ≥99% (TLC) grade) - AppliChem nepiedāvā TLC grade.
2. daļa. Šūnu inkubēšanas vides un piedevas (INVITROGEN vai ekvivalentas).
npk
1
2
3
4
5
6
7
8
9
10
11
12
13
produkts
FETAL BOVINE SERUM
Recovery Cell Culture Freezing Medium - DMSO
DPBS (+)CaCl2 (+) MgCl2
DPBS (-)CaCl2 (-) MgCl2
Versene 1x
HEPES 1M
Glasgov MEM BHK - 21
L- Glutamine 200MM (100x)
DMEM + GlutaMAX
Mayer's Hematoxylin
MEM Non Essential Amino Acids (100X), liquid
Sodium Pyruvate MEM 100 mM, liquid
Kanamycin Sulfate
Invitrogen
10500064
12648010
14040174
14190169
15040033
15630056
21710082
25030024
31966047
008001
11140035
11360039
11815024
Interlux
77,17
88,35
89,82
87,17
8,00
47,48
108,12
8,73
104,08
32,98
10,18
6,98
33,17
9
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
LB Broth Base, powder (Lennox L Broth Base)®
Earle's Balanced Salt Solution (EBSS) (1X), liquid
Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid
Penicillin-Streptomycin, liquid
Fetal Bovine Serum, Certified
RPMI 1640 Medium (1X), liquid
2-YT Broth
L-Glutamine 200 mM (100X), liquid
SELECT Agar®, powder
F-12 Nutrient Mixture (Ham), liquid
Opti-MEM® I Reduced Serum Medium (1X), liquid - with LGlutamine
Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High
Glucose)
PureLink™ Quick Gel Extraction Kit
ProLong® Gold antifade reagent with DAPI - special packaging
Foetal bovine serum, heat inactived
Penicillin-Streptomycin
UltraPure™ Agarose 500 g
UltraPure™ Low Melting Point Agarose100 g
Primārā antiviela, mouse anti-p16
Primārā antiviela, ERK1/2 [pT202/pY204], Rabbit polyclonal,
100 ul
DMEM
Primārā antiviela, AKT/PKB [pS473], Rabbit monoclonal, 100 ul
Collagen I, Rat Tail, RUO, 5mg/ml
Alexa Fluor 594 goat anti—rabbit IgG
Primārā antiviela, cdk4, mouse monoclonal, 100 ug
DAB histochemistry kit with goat anti-mouse IgG-HRP
Goat anti-Rabbit IgG -Alkaline phosphatase, 1 mg
ProLong® Gold antifade reagent with DAPI, 5x2ml
Zeocin™ Selection Reagent, 1g
Vector pTEF1/Zeo
Trypsin, 0.5% (10x) with EDTA 4Na, liquid
Antibiotic-Antimycotic (100X), liquid
12780052
14155048
14190094
15140122
16000044
21875158
22712020
25030024
30391023
31765068
46,49
13,09
10,18
15,74
310,95
113,45
62,38
8,73
108,57
181,98
31985047
23,10
61965059
K210012
P36935
10500064
15070063
16500500
16520100
180432
114,49
85,19
182,84
77,17
7,64
471,44
447,24
305,03
368800
41966052
44621G
A1048301
A11012
AHZ0202
D22185
G21079
P36935
R25001
V50320
15400-054
15240-062
247,66
92,54
300,17
158,19
176,61
249,88
119,89
249,92
182,84
276,06
408,89
37,66
36,03
3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti).
10
npk
Applied Biosystems
ABI #
1
TaqMan® MicroRNA Reverse
Transcription Kit, 200 reactions
4366596
Power SYBR Green PCR Master Mix (2x5)
4368706
High Capacity cDNA Reverse Transcription
Kit with RNase Inhibitor, 200 Reactions
4374966
2
3
4
mirVana™ miRNA Isolation Kit
AM1560
5
DNA-free™
AM1906
RNAlater®
AM7024
Human Placenta Total RNA
AM7950
Acid-Phenol:Chloroform, pH 4.5 (with
IAA, 125:24:1)
AM9720
Phenol:Chloroform:IAA, 25:24:1, pH 6.6
(raise to pH 7.9 with included buffer)
AM9730
RNaseZap® Wipes
AM9786
6
7
8
9
10
Ekvivalents produkts, daudzums, ražotājs, prod. Kods
(Interlux piedāvājums)
miScript Reverse Transcription Kit (50),
218061, Qiagen (komplektā 4 x 218061
gab. = 200 rekcijas)
QuantiTect SYBR Green PCR Kit (200),
204143, Qiagen (komplektā 2 x 204143
gab. = 400 reakcijas)
Omniscript RT Kit (200), 205113, Qiagen
(kompolektā 1 x 205113 gab. = 200
reakcijas)
miRNeasy Mini Kit (50), 217004, Qiagen
(komplektā 1 x 217004 gab. = 50
izdalīšanas)
RNase-Free DNase Set (50), 79254, Qiagen
(komplektā 1 x 79254 gab. = 50
pagatavošanas)
RNAlater RNA Stabilization Reagent (250
mL), 76106, Qiagen (komplektā 1 x 76106
gab. = 250 mL)
Blue-Ribbon RNA Human Placenta total
RNA, 2x50ug, OriGene HT1013
Acid-Phenol:Chloroform, pH 4.5 (with
IAA, 125:24:1), Sigma Aldrich, 77619100ML
Phenol:Chloroform:IAA, 25:24:1, pH 6.6
(raise to pH 7.9 with included buffer),
AppliChem Lifescience A2489.0100
Rnase AWAY wipes (100 gab.) , VWR,
115-1250
Adrona
Interlux
110,00
800,00
357,00
500,00
230,00
400,00
229,00e)
200,00
83,00e)
53,00
169,00
190,00
215,00
377,00
68,00
50,00
68,00
29,00
59,00
120,00
e) Piedāvātie produkti ir ekvivalenti, bet nav pilnībā identiski, tāpēc nolemts līgumu slēgt ar abiem pretendentiem.
4. daļa. Mikrofiltrācijas aksesuāri (MILLIPORE vai ekvivalenti).
npk
1
Aksesuārs
Spin-OUT™ 6,000 Med Columns 0.5ml volume; >10bp cutoff; 10ea
Millipore #
2152
Faneks
65,32
Interlux
84,00
11
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Spin-OUT™ 12,000 Med Columns 0.5ml volume; >20bp cutoff; 10ea
Montage PCR 250/Pk
Millipore Amicon Ultra-4 Centrifugal Filter Unit
Amicon Ultra-15, 10 kD, 24 gab/iep.
Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk
Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk
Amicon Ultra-15, 10 kD, 24 gab/iep.
Amicon filters Ultra-15, 10kDa Ultracel-10 memb, 96/Pk
Amicon Ultra-15, 50 kD, 24 gab/iep.
Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk
Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk
Millipore Amicon Ultra-15 Centrifugal Filter Unit
Amicon filters Ultra-15, 100kDa Ultracel-100 memb, 96/Pk
Ultrafree-0.5 Biomax 10kDa 100/Pk
16
Ultrafree-0.5 Biomax 10kDa 100/Pk
2156
UFC7PC250
UFC810024/UFC
UFC900324
UFC901024
UFC901024
UFC901024
UFC901096
UFC905024
UFC910024
UFC910024
UFC910024/UFC
UFC910096
UFV5BGC00
aiztāts ar
UFC501096
UFV5BGC00
aiztāts ar
UFC501096
17
Ultrafree-0.5 Biomax 100kDa 100/Pk
UFV5BHK00
Ultrafree-0.5 Biomax 100kDa 100/Pk
UFV5BHK00
18
61,77
220,81
(UFC503096)
116,44
159,04
169,69
169,69
169,69
607,05
159,04
160,46
160,46
160,46
621,96
225,07
80,00
480,00
150,00
215,00
210,00
210,00
210,00
735,00
195,00
205,00
205,00
205,00
750,00
275,00
225,07
275,00
220,81
(UFC510096)
220,81
(UFC510096)
275,00
275,00
12
12. Uzvarētāji, ar kuriem nolemts slēgt vispārējo vienošanos, to piedāvātās preces un cenas:
12.1. SIA Interlux
1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti).
npk
1
2
6
7
8
9
10
11
16
18
21
22
23
24
27
28
29
33
35
36
37
Viela
Rubidium chloride
Tryptone, 1 kg
Calcium chloride hexahydrate
Copper(II) sulfate pentahydrate, puriss.
p.a., ACS reagent, reag. ISO, reag. Ph.
Eur., 99-102%
Diethyl pyrocarbonate
MES monohydrate
Sodium chloride, ≥99.5%, BioUltra, for
molecular biology, 5kg
SDS
Trizma base
Agarose
G-418 disulfate salt, 5g
Aluminium oxide Type A-5
Amicase, 1kg
Acrylamide/Bis-acrylamide
Anti-his antivielas peroksidāzes konjugāts
Monoclonal anti-polyHistidine-Peroxidase
antibody from mouse
BSA; pH ~7 (1% in 0.15 M NaCl), ≥98%
(agarose gel electrophoresis), lyophilized
powder (Sigma-Aldrich)
Brilliant Blue R
Boric acid, 1kg
Chloramphenicol, 100g
Carbenicillin, disodium salt, 89.0-100.5%
anhydrous basis
Ekvivalents produkts, daudzums, ražotājs, produkta kods (Interlux)
Rubidium chloride, 25g, Amresco, 0632-25
Tryptone, 1 kg, BD, 211705
Calcium chloride hexahydrate, 500g, AppliChem Lifescience,
A2303.1000
Copper(II) sulfate pentahydrate, pure. p.a., reag. Ph. Eur., 99-101%,
1kg, AppliChem Lifescience, A1367.1000
Diethyl pyrocarbonate, 25ml, Molekula, 11155018
MES monohydrate, 100g, AppliChem Lifescience, A1074.0100
Sodium chloride, ≥99.5%, for molecular biology, 5kg, BDH Prolabo,
443827W
SDS, 100g, AppliChem Lifescience, A1112.0100
Trizma base, 1kg, BDH Prolabo, 28808.294
Agarose, 500g, StarLab GmbH, N3101-0500
G-418 disulfate salt, 5g, AppliChem Lifescience, A2167.0001
Aluminium oxide Type A-5, 500g, Sigma, A2039-500G
Amicase, 1kg, Sigma, A2427-1KG
Acrylamide/Bis-acrylamide, 1L, AppliChem Lifescience, A3658.1000
Anti-his (C-term)antivielas peroksidāzes konjugāts, 1VL, 50 µl,
Invitrogen, R930-25
Monoclonal anti-polyHistidine-Peroxidase antibody from mouse,50
µl, Invitrogen, R930-25
BSA; pH ~7, ≥98% (agarose gel electrophoresis), lyophilized
powder, 100g, AppliChem Lifescience, A1391.0100
Brilliant Blue R, 50g, AppliChem Lifescience, A1092.0025
Boric acid, 1kg, AppliChem Lifescience, A2940.1000
Chloramphenicol, 100g, AppliChem Lifescience, A7495.0100
Carbenicillin, disodium salt, 89.0-100.5% anhydrous basis, 1g,
AppliChem Lifescience, A1491.0001
Cena Ls
69,40
86,20
18,80
23,00
54,70
31,90
29,60
26,20
36,30
164,00
320,40
34,72
112,45
77,18
214,73
214,73
100,00
48,00
24,80
42,20
21,90
13
39
Cesium chloride
40
44
45
51
52
Chamber slides, sterile, 4 wells on glass
EDTA, anhydrous, ≥99% (titration)
EDTA, 500g
Formamide
Fetal Bovine Serum
Formaldehyde (37%) Solution (Formalin),
500 ml
L-Glutathione reduced
D-(+)-galactose
L-Glutamic acid potassium salt
monohydrate
Gold(III) chloride trihydrate, 1g
D-(+)-Glucose, ≥99% (GC)
Glutamine solution
Anti-polyHistidine antibody, monoclonal,
produced in mouse, 0.2 ml
HEPES
Imidazole
Kanamycin sulfate, 50g
LB Broth
Leucine
MOPS
MTT Formazan
mitomycin C
Magnesium acetate
M9 salts 5X
2-Mercaptoethanol
Nutrient Mixture F-10 Ham
53
54
55
56
57
59
60
62
63
65
66
67
68
69
70
72
73
74
75
77
80
82
84
89
Potassium phosphate monobasic, 1kg
Tween 20
PEG 8000
Parafilm® "M"
roll size 2 in. × 250 ft
Cesium chloride, 500g, BDH Prolabo, 441574M
Chamber slides, sterile, 4 wells on glass, 16gab./iepak., Nunc, 7342049
EDTA, anhydrous, ≥99%, 1kg, AppliChem Lifescience, A5097.1000
EDTA, 500g, AppliChem Lifescience, A1103.0500
Formamide, 500ml, AppliChem Lifescience, A0937.0500
Fetal Bovine Serum, 500ml, Invitrogen, 10106169
Formaldehyde (37%) Solution (Formalin), 500 ml, AppliChem
Lifescience, A0877.0500
L-Glutathione reduced, 25g, AppliChem Lifescience, A2084.0025
D-(+)-galactose, 100g, AppliChem Lifescience, A1131.0100
L-Glutamic acid potassium salt monohydrate, 500g, Alfa Aesar,
A17232
Gold(III) chloride trihydrate, 1g, Alfa Aesar, 36400
D-(+)-Glucose, ≥99%, 250g, AppliChem Lifescience, A1422.0500
Glutamine solution, 100ml, Invitrogen, 25030024
Anti-polyHistidine antibody, monoclonal, produced in mouse, 50 µl,
Invitrogen, R930-25
HEPES, 250g, AppliChem Lifescience, A3268.0250
Imidazole, 100g, AppliChem Lifescience, A1073.0100
Kanamycin sulfate, 50g, AppliChem Lifescience, A1493.0050
LB Broth, 1kg, BD, 1231
Leucine, 250g, AppliChem Lifescience, A3460.0250
MOPS, 100g, BDH Prolabo, 443832T
MTT Formazan, 1g, Sigma, M2003-1G
Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002
Magnesium acetate, 250g, AppliChem Lifescience, A1877.0250
M9 salts 5X, 250g, Sigma, M6030-250G
2-Mercaptoethanol, 250ml, BDH Prolabo, 436024C
Nutrient Mixture F-10 Ham, 6x500ml, Biochrom, F0715
Potassium phosphate monobasic, 1kg, AppliChem Lifescience,
A3620.1000
Tween 20, 100ml, BDH Prolabo, 437082Q
PEG 8000, 500g, AppliChem LifescienceA2204.0500
Parafilm® "M"
roll size 2 in. × 250 ft, 1gab., Brand, 291-1214
377,40
97,82
43,50
20,69
13,70
75,00
29,00
74,20
19,00
55,30
75,40
13,60
8,00
214,73
76,70
15,10
144,50
42,90
49,00
26,20
76,00
204,50
19,90
50,37
14,00
38,70
30,30
9,39
13,60
21,90
14
93
94
96
98
100
103
Ribonuclease A Type I-A
Sucrose, for molecular biology, ≥99.5%
(GC), 5kg
Thiamine hydrochloride
Trypsin proteomics grade
108
113
114
115
116
121
122
123
Tryptone
Yeast Extract, 1kg
Carboy with spigot, polypropylene, 20l,
autoclavable
Digitonin
EGTA
HEPES
Mitomycin C
Mitomycin C
ß-Galactosidase Reporter Gene Staning kit
X-Gal
124
Magnesium acetate tetrahydrate
Ribonuclease A Type I-A, 100mg, AppliChem Lifescience,
A2760.0100
Sucrose, for molecular biology, ≥99.5% (GC), 5kg, AppliChem
Lifescience, A2211.5000
Thiamine hydrochloride, 250g, AppliChem Lifescience, A0955.0250
Trypsin proteomics grade, 5x20ug, Sigma, T6567-5X20UG
Tryptone, 250 g, BD, 211921
Yeast Extract, 1kg, BD,
Carboy with spigot, polypropylene, 20l, autoclavable, 1gab., Nunc
Nalgene, 216-8762
Digitonin, 500mg, AppliChem Lifescience, A1905.0500
EGTA, 100g, AppliChem Lifescience, A0878.0100
HEPES, 500g, AppliChem Lifescience, A3268.0500
Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002
Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002
ß-Galactosidase Reporter Gene Staning kit, 1kit, Sigma, GALS-1KT
X-Gal, 100mg, AppliChem Lifescience, A4978.0100
Magnesium acetate tetrahydrate, 1kg, AppliChem Lifescience,
A1877.1000
23,60
103,70
54,20
74,00
55,00
96,00
67,80
99,60
163,30
132,90
204,50
204,50
199,04
18,00
50,20
2. daļa. Šūnu inkubēšanas vides un piedevas (INVITROGEN vai ekvivalentas).
1
2
3
4
5
6
7
8
9
10
11
12
13
14
FETAL BOVINE SERUM
Recovery Cell Culture Freezing Medium - DMSO
DPBS (+)CaCl2 (+) MgCl2
DPBS (-)CaCl2 (-) MgCl2
Versene 1x
HEPES 1M
Glasgov MEM BHK - 21
L- Glutamine 200MM (100x)
DMEM + GlutaMAX
Mayer's Hematoxylin
MEM Non Essential Amino Acids (100X), liquid
Sodium Pyruvate MEM 100 mM, liquid
Kanamycin Sulfate
LB Broth Base, powder (Lennox L Broth Base)®
10500064
12648010
14040174
14190169
15040033
15630056
21710082
25030024
31966047
008001
11140035
11360039
11815024
12780052
77,17
88,35
89,82
87,17
8,00
47,48
108,12
8,73
104,08
32,98
10,18
6,98
33,17
46,49
15
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
Earle's Balanced Salt Solution (EBSS) (1X), liquid
Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid
Penicillin-Streptomycin, liquid
Fetal Bovine Serum, Certified
RPMI 1640 Medium (1X), liquid
2-YT Broth
L-Glutamine 200 mM (100X), liquid
SELECT Agar®, powder
F-12 Nutrient Mixture (Ham), liquid
Opti-MEM® I Reduced Serum Medium (1X), liquid - with L-Glutamine
Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose)
PureLink™ Quick Gel Extraction Kit
ProLong® Gold antifade reagent with DAPI - special packaging
Foetal bovine serum, heat inactived
Penicillin-Streptomycin
UltraPure™ Agarose 500 g
UltraPure™ Low Melting Point Agarose100 g
Primārā antiviela, mouse anti-p16
Primārā antiviela, ERK1/2 [pT202/pY204], Rabbit polyclonal, 100 ul
DMEM
Primārā antiviela, AKT/PKB [pS473], Rabbit monoclonal, 100 ul
Collagen I, Rat Tail, RUO, 5mg/ml
Alexa Fluor 594 goat anti—rabbit IgG
Primārā antiviela, cdk4, mouse monoclonal, 100 ug
DAB histochemistry kit with goat anti-mouse IgG-HRP
Goat anti-Rabbit IgG -Alkaline phosphatase, 1 mg
ProLong® Gold antifade reagent with DAPI, 5x2ml
Zeocin™ Selection Reagent, 1g
Vector pTEF1/Zeo
Trypsin, 0.5% (10x) with EDTA 4Na, liquid
Antibiotic-Antimycotic (100X), liquid
14155048
14190094
15140122
16000044
21875158
22712020
25030024
30391023
31765068
31985047
61965059
K210012
P36935
10500064
15070063
16500500
16520100
180432
368800
41966052
44621G
A1048301
A11012
AHZ0202
D22185
G21079
P36935
R25001
V50320
15400-054
15240-062
13,09
10,18
15,74
310,95
113,45
62,38
8,73
108,57
181,98
23,10
114,49
85,19
182,84
77,17
7,64
471,44
447,24
305,03
247,66
92,54
300,17
158,19
176,61
249,88
119,89
249,92
182,84
276,06
408,89
37,66
36,03
3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti).
npk
Applied Biosystems
4
mirVana™ miRNA Isolation Kit
Ekvivalents produkts, daudzums, ražotājs, prod. Kods (Interlux piedāvājums)
Cena Ls
miRNeasy Mini Kit (50), 217004, Qiagen (komplektā 1 x 217004 gab. =
50 izdalīšanas)
200,00
16
5
DNA-free™
Acid-Phenol:Chloroform, pH 4.5 (with
IAA, 125:24:1)
Phenol:Chloroform:IAA, 25:24:1, pH 6.6
(raise to pH 7.9 with included buffer)
8
9
RNase-Free DNase Set (50), 79254, Qiagen (komplektā 1 x 79254 gab. =
50 pagatavošanas)
Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1), Sigma Aldrich,
77619-100ML
Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with included
buffer), AppliChem Lifescience A2489.0100
53,00
50,00
29,00
12.2. SIA Labochema Latvija:
1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti).
npk
3
4
5
12
13
14
15
17
19
20
25
26
30
Viela
o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
cycloheximide
Minisart-Plus filters 0.45µm, 50/pack
PEG 1000
PEG 200
PEG 300
PEG 400
PEG 4000
Anti-trušu antivielas peroksidāzes konjugāts
Agar
L-(+)-Arabinose
L-(+)-Arabinose, 500g
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat. IgG fraction of
antiserum, buffered aqueous solution, 1 ml
31
Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit. IgG fraction of
antiserum, buffered aqueous solution, 2 ml
32
34
38
41
42
43
46
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
SIGMAFAST BCIP/NTB, 25 tabletes
CelLytic™ M
NZ-Amine A, 1 kg
DMSO
DMEM- high glucose
Fibronectin solution from human fibroblasts, 100 UL
Sigma
P6912-100TAB
01810-5G
17829K
81188-250G
88440-250ML-F
90878-250ML-F
91893-250ML-F
95904-250G-F
A0545-1ML
A1296-100G
A3256-100G
A3256-500G
200,46
95,58
73,00
13,24
10,73
14,03
12,22
11,01
128,13
43,57
71,79
287,75
A8275 - 1ML
75,12
A9044 - 2ML
114,43
A9169-2ML
B5655-25TAB
C2978-250ML
C7290
D2650-100ML
D6429-6X500ML
F0556-100UL
135,66
128,38
137,37
82,03
101,65
21,60
124,36
Cena Ls
17
47
F2006-1MG
133,90
48
49
50
52
58
61
64
71
76
78
79
81
83
85
86
87
88
90
91
92
95
97
99
101
102
104
105
106
107
Fibronectin from human plasma cell culture tested, lyophilized powder, 1mg
Anti-Fibronectin antibody produced in rabbit. affinity isolated antibody, buffered aqueous solution,
0.2ml
Freund`s Adjuvant, incomplete
Freund`s Adjuvant, complete
Fetal Bovine Serum
Sephadex® G-50, Superfine
Hepes
Iscove's modified dulbecco's medium
Nocodazole
Mouse monoclonal anti-Nanog IgG1, Clone NNG-811, 25 ul
Nitrocellulose membrane for blotting, 0,45mkm, 15x15cm
Glass slides coated with poly-L-lysine
Pepticase, 250 g
Tween 20
PEG 3350
Phosphate buffered saline powder, pH 7.4
Phosphate buffered saline
Phosphate-Citrate Buffer
Protein A–Peroxidase from Staphylococcus aureus/horseradish, 1 mg
RPMI 1640 Medium
Retinoic acid
Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0%
Trypsin-EDTA solution
Tryptose Phosphate Broth 1x
TRI – Reagent
TRI Reagent, 200 ml
Plastibrand PD-tips 1.25ml
Plastibrand PD-tips 2.5ml
Plastibrand PD-tips 5ml
Nalgene® vacuum filtration system capacity 150 mL, pore size 0.2 μm, 12 pcs.
F3648-.2ML
F5506-6X10ML
F5881-6X10ML
F7524-500ML
G5050-50G
H0887-100ML
I3390-6X500ML
M1404-2MG
N3038
N8267-5EA
P0425-72EA
P1192
P1379-1L
P3640-500g
P3813-10PAK
P4417-100TAB
P4809-100TAB
P8651-1MG
R0883-6x500ML
R2625-50MG
S4641-500G
T4049-100ML
T8159-100ML
T9424-100ML
T9424-200ML
Z332631-1PAK
Z332658-1PAK
Z332666-1PAK
Z358185-1CS
45,30
77,60
77,23
79,65
132,88
34,14
100,15
20,01
49,74
74,54
36,90
51,48
34,88
15,06
38,82
69,11
156,49
185,95
59,82
17,60
21,75
9,01
10,52
143,23
245,18
40,55
40,55
40,55
63,10
109
110
111
Nalgene® vacuum filtration system capacity 250 mL, pore size 0.2 μm, 12 pcs.
Hanna pH standard buffer solution pH 4.01, 500ML
Hanna pH standard buffer solution pH 7.01, 500ML
Z370606-1CS
Z655112-500ML
Z655139-500ML
70,80
21,24
21,24
18
112
117
118
109
120
125
Hanna pH standard buffer solution pH 10.01, 500ML
Cycloheximide
OptiPrep Density Gradient Medium
MG-132
Lactacystine
Glycine (ReagentPlus®, ≥99% (TLC) grade)
Z655155-500ML
01810-5G
D1556-250ML
C2211-5MG
L6785-2MG
G7126-1KG
21,24
102,94
198,43
84,85
225,35
39,19
12.3. SIA Adrona:
3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti).
npk
Applied Biosystems
1
2
3
4
5
6
7
10
TaqMan® MicroRNA Reverse Transcription Kit, 200 reactions
Power SYBR Green PCR Master Mix (2x5)
High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 Reactions
mirVana™ miRNA Isolation Kit
DNA-free™
RNAlater®
Human Placenta Total RNA
RNaseZap® Wipes
ABI #
Cena Ls
4366596
4368706
4374966
AM1560
AM1906
AM7024
AM7950
AM9786
110,00
357,00
230,00
229,00
83,00
169,00
215,00
59,00
12.4. SIA Faneks:
4. daļa. Mikrofiltrācijas aksesuāri (MILLIPORE vai ekvivalenti).
npk
1
2
3
4
5
6
7
8
9
10
Aksesuārs
Spin-OUT™ 6,000 Med Columns 0.5ml volume; >10bp cutoff; 10ea
Spin-OUT™ 12,000 Med Columns 0.5ml volume; >20bp cutoff; 10ea
Montage PCR 250/Pk
Millipore Amicon Ultra-4 Centrifugal Filter Unit
Amicon Ultra-15, 10 kD, 24 gab/iep.
Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk
Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk
Amicon Ultra-15, 10 kD, 24 gab/iep.
Amicon filters Ultra-15, 10kDa Ultracel-10 memb, 96/Pk
Amicon Ultra-15, 50 kD, 24 gab/iep.
Millipore #
2152
2156
UFC503096
UFC810024/UFC
UFC900324
UFC901024
UFC901024
UFC901024
UFC901096
UFC905024
Cena Ls
65,32
61,77
220,81
116,44
159,04
169,69
169,69
169,69
607,05
159,04
19
11
12
13
14
15
16
17
18
Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk
Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk
Millipore Amicon Ultra-15 Centrifugal Filter Unit
Amicon filters Ultra-15, 100kDa Ultracel-100 memb, 96/Pk
Ultrafree-0.5 Biomax 10kDa 100/Pk
Ultrafree-0.5 Biomax 10kDa 100/Pk
Ultrafree-0.5 Biomax 100kDa 100/Pk
Ultrafree-0.5 Biomax 100kDa 100/Pk
UFC910024
UFC910024
UFC910024/UFC
UFC910096
UFC501096
UFC501096
UFC510096
UFC510096
160,46
160,46
160,46
621,96
225,07
225,07
220,81
220,81
20
21
12. Pretendenti, ar kuriem nolemts slēgt līgumu:
Iepirkuma komisija nolemj slēgt Vispārējās vienošanās līgumu ar Interlux SIA, Labochema
Latvija SIA, Faneks SIA un Adrona SIA par atbilstošo preču piegādi.
13. Lēmuma pamatojums:
Pretendenti un to piedāvājumi pilnībā atbilst Iepirkumu procedūrā izvirzītajām prasībām un
piedāvājumam ir zemākā cena.
14. Valsts pasūtītāja pilnvarotā komisija:
9.1. Latvijas Biomedicīnas pētījumu un studiju centra “NOLIKUMS par iepirkumu Latvijas
Biomedicīnas pētījumu un studiju centra vajadzībām” (3.02.2007).
Direktora rīkojumi Nr. 7-S/04 (17.08.2004.) un 15-S/08 (18.08.2008).
9.2. Iepirkuma komisijas darbs un tās attiecības ar pretendentu tiek organizētas saskaņā ar
“Publisko iepirkumu likumu” un attiecīgajiem MK nolikumiem.
Datums: 23. 07. 2010
Valsts pasūtītāja pilnvarotā komisija:
Ēriks Jankevics
Andris Zeltiņš
Svetlana Kazmerika
Irēna Čudarāne
Pateicamies visiem pretendentiem par piedalīšanos cenu aptaujā.
22