Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
APP Latvijas Biomedicīnas pētījumu un studiju centra Iepirkumu komisijas Z I Ņ O J U M S Nr. 24 Rīga 2010. gada 23. jūlijā 1. Iepirkuma identifikācijas numurs: BMC2010/3_ESF 2. Ievietots internetā: 27.05.2010 3. Valsts pasūtītājs: APP Latvijas Biomedicīnas pētījumu un studiju centrs, adrese: Rātsupītes1, Rīga LV 1067, Latvija, PVN LV90002120158, Tālrunis (371) 67808216, Telefakss (371) 67442407, E-mail: [email protected] 4. Valsts pasūtījuma priekšmets: Materiālu un molekulārās bioloģijas reaģentu piegāde. Paredzēts noslēgt vispārējo vienošanos par atbilstošo preču piegādi uz 30 mēnešiem no vienošanās noslēgšanas brīža. Preču kopējā vērtība: līdz 87887,99 latu periodā. 5. Valsts pasūtījuma piešķiršanas metode – Atklāts konkurss. Metodes izvēles pamatojums: Valsts pasūtījuma raksturs un kopējā vērtība atbilst " Publisko iepirkumu likuma" un tam pakārtoto informatīvo aktu nosacījumiem. 6. Prasības pretendentiem: 6.1. Reģistrācijas apliecības kopiju vai izziņas, kas apliecina, ka pretendents ir reģistrēts likumā noteiktajā kārtībā. 6.2. Rakstisks apliecinājums, ka uz pretendentu neattiecas no Publisko iepirkumu likuma 39.panta pirmajā daļā minētajiem nosacījumiem (ņemot vērā šā panta trešajā daļā minētos termiņus). Konkursa uzvarētājiem ir jābūt gataviem iesniegt informāciju atbilstoši Publisko iepirkumu likuma 39. panta sestās daļas regulējumam. 6.3. Ja pasūtītājam ir radušās šaubas par pretendenta reputāciju, tas ir tiesīgs no pretendenta papildus pieprasīt nepieciešamo informāciju par tā komercdarbību likumā paredzētajā kārtībā. 7. Vērtēšanas kritēriji: Piedāvājums ar viszemāko cenu. 8. Piedāvājumu iesniegšanas vieta un termiņš: Piedāvājumi iesniedzami līdz 2010. g. 1. jūlijam APP Latvijas Biomedicīnas pētījumu un studiju centrā Rātsupītes ielā 1, Rīgā, sekretariātā Svetlanai Kazmerikai darba dienās no 1000 līdz 1500. Piedāvājuma derīguma laiks tiek noteikts 60 kalendārās dienas no piedāvājuma atvēršanas brīža. 9. Piedāvājumu atvēršanas vieta un laiks: APP Latvijas Biomedicīnas pētījumu un studiju centrs, 2010.g. 1. jūlijs plkst. 1500. 10. Piedāvājuma atvēršanā klātesošās personas: 1 Iepirkumu komisijas locekļi, kā arī sekojošie pretendentu pārstāvji (attiecīgi sarakstam pielikumā): Aleksandrs Šepteličs, Interlux SIA Sergejs Andrejevs, Interlux SIA 2 11. Pretendentu iesniegto preču cenu saraksts: 1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti). npk Viela 1 2 Rubidium chloride Tryptone, 1 kg 3 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet 4 cycloheximide 5 Minisart-Plus filters 0.45µm, 50/pack 6 7 8 9 Calcium chloride hexahydrate Copper(II) sulfate pentahydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99-102% Diethyl pyrocarbonate Sigma 83979 T9410 P6912-100TAB 01810-5G 17829K 21108-500G 31293-1KG 40718-25ML 10 MES monohydrate Sodium chloride, ≥99.5%, BioUltra, for molecular biology, 5kg 69892-100G 11 12 13 SDS PEG 1000 PEG 200 71729-100G 81188-250G 88440-250ML-F 14 PEG 300 90878-250ML-F 15 16 17 18 19 PEG 400 Trizma base PEG 4000 Agarose Anti-trušu antivielas peroksidāzes konjugāts 91893-250ML-F 93349-1KG 95904-250G-F A9539-500G A0545-1ML 71376-5KG Ekvivalents produkts, daudzums, ražotājs, produkta kods (Interlux) Rubidium chloride, 25g, Amresco, 0632-25 Tryptone, 1 kg, BD, 211705 o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet, 100 tab, Sigma, P6912-100TAB Cycloheximide, 5g, AppliChem Lifescience, A0879.0005 Minisart-Plus filters 0.45µm, 50/pack, Sartorius, 17829K Calcium chloride hexahydrate, 500g, AppliChem Lifescience, A2303.1000 Copper(II) sulfate pentahydrate, pure. p.a., reag. Ph. Eur., 99-101%, 1kg, AppliChem Lifescience, A1367.1000 Diethyl pyrocarbonate, 25ml, Molekula, 11155018 MES monohydrate, 100g, AppliChem Lifescience, A1074.0100 Sodium chloride, ≥99.5%, for molecular biology, 5kg, BDH Prolabo, 443827W SDS, 100g, AppliChem Lifescience, A1112.0100 PEG 1000, 250g, Alfa Aesar, B22134 PEG 200, 250ml, Alfa Aesar, B21918 PEG 300, 250ml, AppliChem Lifescience, A5537.0250 PEG 400, 250ml, AppliChem Lifescience, A2203.0500 Trizma base, 1kg, BDH Prolabo, 28808.294 PEG 4000, 250g, Alfa Aesar, A16151 Agarose, 500g, StarLab GmbH, N3101-0500 Anti-trušu antivielas peroksidāzes konjugāts, Interlux Labochema 69,40 86,20 99,26 161,46 260,00 200,46 104,05 95,58 77,50 73,00 18,80 20,99 23,00 40,77 54,70 69,92 31,90 55,77 29,60 68,80 26,20 14,40 14,00 31,84 13,24 10,73 14,20 14,03 13,60 36,30 17,00 164,00 179,09 12,22 132,15 11,01 415,82 128,13 3 20 Agar A1296-100G 21 G-418 disulfate salt, 5g 22 23 Aluminium oxide Type A-5 Amicase, 1kg A2039-500G A2427 24 25 26 Acrylamide/Bis-acrylamide L-(+)-Arabinose L-(+)-Arabinose, 500g A2729-1L A3256-100G A3256-500G 27 Anti-his antivielas peroksidāzes konjugāts A1720-5G A7058-1VL 31 Monoclonal anti-polyHistidine-Peroxidase antibody from mouse BSA; pH ~7 (1% in 0.15 M NaCl), ≥98% (agarose gel electrophoresis), lyophilized powder (Sigma-Aldrich) Anti-Rabbit IgG (whole molecule)– Peroxidase antibody produced in goat. IgG fraction of antiserum, buffered aqueous solution, 1 ml Anti-Mouse IgG (whole molecule)– Peroxidase antibody produced in rabbit. IgG fraction of antiserum, buffered aqueous solution, 2 ml 32 Anti-Rabbit IgG (whole molecule)– Peroxidase antibody produced in goat A9169-2ML 33 Brilliant Blue R B7920-50G 34 SIGMAFAST BCIP/NTB, 25 tabletes 35 Boric acid, 1kg B6768-1KG 36 Chloramphenicol, 100g C0378-100G 28 29 30 A7058-1VL A7906-100G A8275 - 1ML A9044 - 2ML B5655-25TAB 1mg, Invitrogen, G-21234 Agar, 100g, BD, 214050 G-418 disulfate salt, 5g, AppliChem Lifescience, A2167.0001 Aluminium oxide Type A-5, 500g, Sigma, A2039-500G Amicase, 1kg, Sigma, A2427-1KG Acrylamide/Bis-acrylamide, 1L, AppliChem Lifescience, A3658.1000 L-(+)-Arabinose, 100g, Molekula, 30957927 L-(+)-Arabinose, 500g, Molekula, 30957927 Anti-his (C-term)antivielas peroksidāzes konjugāts, 1VL, 50 µl, Invitrogen, R930-25 Monoclonal anti-polyHistidine-Peroxidase antibody from mouse,50 µl, Invitrogen, R93025 BSA; pH ~7, ≥98% (agarose gel electrophoresis), lyophilized powder, 100g, AppliChem Lifescience, A1391.0100 Anti-Rabbit IgG (whole molecule)– Peroxidase antibody produced in goat. IgG fraction of antiserum, buffered aqueous solution, 1mg, Invitrogen, G-21234 Anti-Mouse IgG (whole molecule)– Peroxidase antibody produced in goat. IgG fraction of antiserum, buffered aqueous solution, 1mg, Invitrogen, G-21040 Anti-Rabbit IgG (whole molecule)– Peroxidase antibody produced in goat, 1mg, Invitrogen, G-21234 Brilliant Blue R, 50g, AppliChem Lifescience, A1092.0025 SIGMAFAST BCIP/NTB, 25 tabletes, Sigma, B5655-25TAB Boric acid, 1kg, AppliChem Lifescience, A2940.1000 Chloramphenicol, 100g, AppliChem Lifescience, A7495.0100 54,90 43,57 320,40 322,28 34,72 112,45 39,77 128,67 77,18 77,70 388,60 173,05 71,79 287,75 214,73 258,43 214,73 258,43 100,00 124,46 179,09 75,12 172,09 114,43 179,09 135,66 48,00 53,20 143,00 128,38 24,80 31,33 42,20 58,96 4 37 38 Carbenicillin, disodium salt, 89.0-100.5% anhydrous basis CelLytic™ M 39 Cesium chloride 40 41 42 Chamber slides, sterile, 4 wells on glass NZ-Amine A, 1 kg DMSO 43 DMEM- high glucose 44 EDTA, anhydrous, ≥99% (titration) EDS-1KG 45 EDS-500G 48 EDTA, 500g Fibronectin solution from human fibroblasts, 100 UL Fibronectin from human plasma cell culture tested, lyophilized powder, 1mg Anti-Fibronectin antibody produced in rabbit. affinity isolated antibody, buffered aqueous solution, 0.2ml 49 Freund`s Adjuvant, incomplete F5506-6X10ML 50 Freund`s Adjuvant, complete F5881-6X10ML 51 Formamide F7503-500ML 52 F7524-500ML 53 Fetal Bovine Serum Formaldehyde (37%) Solution (Formalin), 500 ml 54 L-Glutathione reduced G4251-25G 55 D-(+)-galactose L-Glutamic acid potassium salt monohydrate G0625-100G 46 47 56 C1389-1G C2978-250ML C3139-500G C6807-1PAK C7290 D2650-100ML D6429-6X500ML F0556-100UL F2006-1MG F3648-.2ML F8775-500ML G1501-500G Carbenicillin, disodium salt, 89.0-100.5% anhydrous basis, 1g, AppliChem Lifescience, A1491.0001 CelLytic™ M, 250ml, Sigma, C2978-250ML Cesium chloride, 500g, BDH Prolabo, 441574M Chamber slides, sterile, 4 wells on glass, 16gab./iepak., Nunc, 734-2049 NZ-Amine A, 1 kg, Amresco, EJ853 DMSO, 100ml, Sigma, D2650-100ML DMEM- high glucose, 6x500ml, Invitrogen, 31966021 EDTA, anhydrous, ≥99%, 1kg, AppliChem Lifescience, A5097.1000 EDTA, 500g, AppliChem Lifescience, A1103.0500 Fibronectin solution from human fibroblasts, 100 UL, Sigma, F0556-100UL Fibronectin Natural Human, 1 mg. From human plasma, Invitrogen, PHE0023 Anti-Fibronectin antibody produced in rabbit. affinity isolated antibody, buffered aqueous solution, 0.2ml, Sigma, F3648-.2ML Freund`s Adjuvant, incomplete, 6x10ml, Sigma, F5506-6X10ML Freund`s Adjuvant, complete, 6x10ml, Sigma, F5881-6X10ML Formamide, 500ml, AppliChem Lifescience, A0937.0500 Fetal Bovine Serum, 500ml, Invitrogen, 10106169 Formaldehyde (37%) Solution (Formalin), 500 ml, AppliChem Lifescience, A0877.0500 L-Glutathione reduced, 25g, AppliChem Lifescience, A2084.0025 D-(+)-galactose, 100g, AppliChem Lifescience, A1131.0100 L-Glutamic acid potassium salt monohydrate, 500g, Alfa Aesar, A17232 21,90 145,00 94,95 137,37 377,40 601,02 97,82 99,00 115,09 133,92 82,03 101,65 66,00 21,60 43,50 72,53 20,69 35,52 131,00 124,36 182,39 133,90 60,00 45,30 103,00 77,60 102,00 77,23 13,70 18,27 75,00 79,65a) 29,00 40,99 74,20 103,66 19,00 23,10 55,30 114,38 5 57 Gold(III) chloride trihydrate, 1g G4022-1G 58 Sephadex® G-50, Superfine G5050-50G 59 D-(+)-Glucose, ≥99% (GC) G5400-250G 60 61 Glutamine solution Hepes 62 Anti-polyHistidine antibody, monoclonal, produced in mouse, 0.2 ml 63 HEPES G7513-100ML H0887-100ML H1029-.2ML H3375-250G 64 Iscove's modified dulbecco's medium 65 Imidazole I0125-100G 66 67 Kanamycin sulfate, 50g LB Broth K4000-50G L3022-1KG 68 69 70 Leucine I3390-6X500ML MOPS MTT Formazan L8000-250G M1254-100G M2003-1G 71 Nocodazole M1404-2MG 72 mitomycin C M4287-5X2MG 73 74 75 Magnesium acetate M5661-250G M9 salts 5X M6030-250G 76 2-Mercaptoethanol Mouse monoclonal anti-Nanog IgG1, Clone NNG-811, 25 ul 77 78 Nutrient Mixture F-10 Ham Nitrocellulose membrane for blotting, M6250-250ML N3038 N6013-6x500ML N8267-5EA Gold(III) chloride trihydrate, 1g, Alfa Aesar, 36400 Sephadex® G-50, Superfine, 50g, GE Healthcare, 17-0041-01 D-(+)-Glucose, ≥99%, 250g, AppliChem Lifescience, A1422.0500 Glutamine solution, 100ml, Invitrogen, 25030024 Hepes, 100ml, Invitrogen, 15630056 Anti-polyHistidine antibody, monoclonal, produced in mouse, 50 µl, Invitrogen, R93025 HEPES, 250g, AppliChem Lifescience, A3268.0250 Iscove's modified dulbecco's medium, 6 x 500ml, Invitrogen, 31980022 Imidazole, 100g, AppliChem Lifescience, A1073.0100 Kanamycin sulfate, 50g, AppliChem Lifescience, A1493.0050 LB Broth, 1kg, BD, 1231 Leucine, 250g, AppliChem Lifescience, A3460.0250 MOPS, 100g, BDH Prolabo, 443832T MTT Formazan, 1g, Sigma, M2003-1G Nocodazole, 2mg, AppliChem Lifescience, A1999.0010 Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002 Magnesium acetate, 250g, AppliChem Lifescience, A1877.0250 M9 salts 5X, 250g, Sigma, M6030-250G 2-Mercaptoethanol, 250ml, BDH Prolabo, 436024C Mouse monoclonal anti-Nanog IgG1, Clone NNG-811, 25 ul, Sigma, N3038-25UL Nutrient Mixture F-10 Ham, 6x500ml, Biochrom, F0715 Nitrocellulose membrane for blotting, 75,40 95,20 292,50 132,88 13,60 18,75 8,00 43,00 14,11 34,14 214,73 229,07 76,70 104,93 108,00 100,15 15,10 26,71 144,50 42,90 399,95 63,57 49,00 26,20 76,00 63,18 59,48 57,44 54,10 20,01 204,50 297,14 19,90 50,37 30,97 46,15 14,00 19,66 52,50 49,74 38,70 76,40 41,67 74,54 6 0,45mkm, 15x15cm 79 Glass slides coated with poly-L-lysine P0425-72EA 80 81 82 Potassium phosphate monobasic, 1kg Pepticase, 250 g Tween 20 P0662-1KG P1192 P1379-100ML 83 Tween 20 P1379-1L 84 85 PEG 8000 PEG 3350 P2139-500g P3640-500g 86 Phosphate buffered saline powder, pH 7.4 P3813-10PAK 87 Phosphate buffered saline P4417-100TAB 88 Phosphate-Citrate Buffer P4809-100TAB 89 Parafilm® "M" roll size 2 in. × 250 ft P7543-1EA 90 Protein A–Peroxidase from Staphylococcus aureus/horseradish, 1 mg P8651-1MG 91 RPMI 1640 Medium 92 Retinoic acid R2625-50MG 93 R4875-100MG 95 Ribonuclease A Type I-A Sucrose, for molecular biology, ≥99.5% (GC), 5kg Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0% 96 Thiamine hydrochloride T4625-250G 97 Trypsin-EDTA solution T4049-100ML 94 R0883-6x500ML S0389-5KG S4641-500G 0,45mkm, 15x15cm, 5gab., Whatman, 7320354 Glass slides coated with poly-L-lysine, 72gab., Sigma, P0425-72EA Potassium phosphate monobasic, 1kg, AppliChem Lifescience, A3620.1000 Pepticase, 250 g, Sigma, P1192-250G Tween 20, 100ml, BDH Prolabo, 437082Q Tween 20, 1L, AppliChem Lifescnience, A4974.1000 PEG 8000, 500g, AppliChem LifescienceA2204.0500 PEG 3350, 500g, Sigma, P3640-500G Phosphate buffered saline powder, pH 7.4, 10pak, Sigma, P3813-10PAK Phosphate buffered saline, 100tab, Amresco, E404-100 Phosphate-Citrate Buffer, 100tab, Sigma, P4809-100TAB Parafilm® "M" roll size 2 in. × 250 ft, 1gab., Brand, 2911214 Protein A–Peroxidase from Staphylococcus aureus/horseradish, 1 mg, Sigma, P86511MG RPMI 1640 Medium, 6x500ml, Invitrogen, 11835063 Retinoic acid, 50mg, AppliChem Lifescience, A6947.0100 Ribonuclease A Type I-A, 100mg, AppliChem Lifescience, A2760.0100 Sucrose, for molecular biology, ≥99.5% (GC), 5kg, AppliChem Lifescience, A2211.5000 Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0%, 500g, Alfa Aesar, A12274 Thiamine hydrochloride, 250g, AppliChem Lifescience, A0955.0250 Trypsin-EDTA solution, 100ml, Sigma, T4049-100ML 48,50 36,90 30,30 54,00 9,39 37,67 51,48 15,00 40,40 34,88 13,60 16,41 18,45 15,06 42,39 38,83 42,00b) 69,11 166,00 156,49 21,90 22,78 195,00 183,95 105 59,82 23,00 17,60 23,60 48,02 103,70 158,94 14,40c) 21,75 54,20 99,13 11,90 9,01 7 98 Trypsin proteomics grade T6567-5X20UG 99 Tryptose Phosphate Broth 1x T8159-100ML 100 101 102 103 Tryptone TRI – Reagent TRI Reagent, 200 ml Yeast Extract, 1kg T9410-250G T9424-100ML T9424-200ML Y1625 104 Plastibrand PD-tips 1.25ml Z332631-1PAK 105 Plastibrand PD-tips 2.5ml Z332658-1PAK 106 Plastibrand PD-tips 5ml Z332666-1PAK 107 Nalgene® vacuum filtration system capacity 150 mL, pore size 0.2 μm, 12 pcs. Z358185-1CS 108 Carboy with spigot, polypropylene, 20l, autoclavable Z360260-1EA 112 Nalgene® vacuum filtration system capacity 250 mL, pore size 0.2 μm, 12 pcs. Hanna pH standard buffer solution pH 4.01, 500ML Hanna pH standard buffer solution pH 7.01, 500ML Hanna pH standard buffer solution pH 10.01, 500ML 113 Digitonin D141-500MG 114 EGTA E3889-100G 115 HEPES H4034-500G 116 117 Mitomycin C Cycloheximide 109 110 111 Z370606-1CS Z655112-500ML Z655139-500ML Z655155-500ML M4287-5X2MG 01810-5G Trypsin proteomics grade, 5x20ug, Sigma, T6567-5X20UG Tryptose Phosphate Broth 1x, 100ml, Sigma, T8159-100ML Tryptone, 250 g, BD, 211921 TRI – Reagent, 100ml, Sigma, T9424-100ML TRI Reagent, 200 ml, Sigma, T9424-200ML Yeast Extract, 1kg, BD, Plastibrand PD-tips 1.25ml, 1pak(100gab.), Brand, 612-5802 Plastibrand PD-tips 2.5ml, 1pak(100gab.), Brand, 612-5803 Plastibrand PD-tips 5ml, 1pak(100gab.), Brand, 612-5804 Nalgene® vacuum filtration system capacity 150 mL, pore size 0.2 μm, 12 pcs., Nunc Nalgene, 514-0024 Carboy with spigot, polypropylene, 20l, autoclavable, 1gab., Nunc Nalgene, 2168762 Nalgene® vacuum filtration system capacity 250 mL, pore size 0.2 μm, 12 pcs., Nunc Nalgene, 514-0027 Hanna pH standard buffer solution pH 4.01, 500ML, Hanna, HI6004 Hanna pH standard buffer solution pH 7.01, 500ML, Hanna, HI6007 Hanna pH standard buffer solution pH 10.01, 500ML, Hanna, HI6010 Digitonin, 500mg, AppliChem Lifescience, A1905.0500 EGTA, 100g, AppliChem Lifescience, A0878.0100 HEPES, 500g, AppliChem Lifescience, A3268.0500 Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002 Cycloheximide, 5g, AppliChem Lifescience, 74,00 55,82 15,00 10,52 55,00 177,00 320,00 96,00 57,40 143,23 245,18 266,93 41,40 40,55 41,40 40,55 41,40 40,55 74,40 63,10 67,80 98,47 83,40 70,80 31,50 21,24 31,50 21,24 31,50 21,24 99,60 125,19 163,30 185,01 132,90 167,53 204,50 104,00 297,14 102,94 8 A0879.0005 OptiPrep Density Gradient Medium, 250ml, 118 OptiPrep Density Gradient Medium D1556-250ML Sigma, D1556-250ML 216,74 198,43 109 MG-132 C2211-5MG MG-132, 5mg, Sigma, C2211-5MG 112,00 84,85 120 Lactacystine L6785-2MG Lactacystine, 0,2mg, Cayman, 70980-0.5 313,40 225,35 Mitomycin C, 5x2mg, AppliChem Lifescience, 121 Mitomycin C M4287-5X2MG A2190.0002 204,50 297,14 ß-Galactosidase Reporter Gene Staning kit, 122 ß-Galactosidase Reporter Gene Staning kit GALS-1KT 1kit, Sigma, GALS-1KT 199,04 219,65 X-Gal, 100mg, AppliChem Lifescience, 123 X-Gal A4978.0100 18,00 B4252-100MG 29,30 Magnesium acetate tetrahydrate, 1kg, 124 Magnesium acetate tetrahydrate M2545-1KG AppliChem Lifescience, A1877.1000 50,20 133,89 Glycine (ReagentPlus®, ≥99%), 1kg, 125 Glycine (ReagentPlus®, ≥99% (TLC) grade) G7126-1KG AppliChem Lifescience, A1067.1000 27,40d) 39,19 a) Fetal Bovine Serum ir bioloģiskas izcelsmes substance un tā var atšķirties atkarībā no ražotāja. Tāpēc līgumu nolemts slēgt ar abiem piegādātājiem. b) PBS Amresco piedāvā to pašu tablešu skaitu par zemāku cenu, taču viņu 1 tablete ir jāšķīdina 100ml, kamēr Sigmas - 200ml, reāli sanāk 2x mazāk PBSa un Sigmas ir lētāks; c) Sodium citrate tribasic dihydrate, ACS reagent, =99.0%. Tika prasīts ACS grade, bet tas netiek piedāvāts (t.i. Alfa Aesar mājas lapā ir atrodams arī ACS grade nātrija citrāts (zem cita koda), bet jau par pavisam citu cenu); d) Glycine (ReagentPlus®, ≥99% (TLC) grade) - AppliChem nepiedāvā TLC grade. 2. daļa. Šūnu inkubēšanas vides un piedevas (INVITROGEN vai ekvivalentas). npk 1 2 3 4 5 6 7 8 9 10 11 12 13 produkts FETAL BOVINE SERUM Recovery Cell Culture Freezing Medium - DMSO DPBS (+)CaCl2 (+) MgCl2 DPBS (-)CaCl2 (-) MgCl2 Versene 1x HEPES 1M Glasgov MEM BHK - 21 L- Glutamine 200MM (100x) DMEM + GlutaMAX Mayer's Hematoxylin MEM Non Essential Amino Acids (100X), liquid Sodium Pyruvate MEM 100 mM, liquid Kanamycin Sulfate Invitrogen 10500064 12648010 14040174 14190169 15040033 15630056 21710082 25030024 31966047 008001 11140035 11360039 11815024 Interlux 77,17 88,35 89,82 87,17 8,00 47,48 108,12 8,73 104,08 32,98 10,18 6,98 33,17 9 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 LB Broth Base, powder (Lennox L Broth Base)® Earle's Balanced Salt Solution (EBSS) (1X), liquid Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid Penicillin-Streptomycin, liquid Fetal Bovine Serum, Certified RPMI 1640 Medium (1X), liquid 2-YT Broth L-Glutamine 200 mM (100X), liquid SELECT Agar®, powder F-12 Nutrient Mixture (Ham), liquid Opti-MEM® I Reduced Serum Medium (1X), liquid - with LGlutamine Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) PureLink™ Quick Gel Extraction Kit ProLong® Gold antifade reagent with DAPI - special packaging Foetal bovine serum, heat inactived Penicillin-Streptomycin UltraPure™ Agarose 500 g UltraPure™ Low Melting Point Agarose100 g Primārā antiviela, mouse anti-p16 Primārā antiviela, ERK1/2 [pT202/pY204], Rabbit polyclonal, 100 ul DMEM Primārā antiviela, AKT/PKB [pS473], Rabbit monoclonal, 100 ul Collagen I, Rat Tail, RUO, 5mg/ml Alexa Fluor 594 goat anti—rabbit IgG Primārā antiviela, cdk4, mouse monoclonal, 100 ug DAB histochemistry kit with goat anti-mouse IgG-HRP Goat anti-Rabbit IgG -Alkaline phosphatase, 1 mg ProLong® Gold antifade reagent with DAPI, 5x2ml Zeocin™ Selection Reagent, 1g Vector pTEF1/Zeo Trypsin, 0.5% (10x) with EDTA 4Na, liquid Antibiotic-Antimycotic (100X), liquid 12780052 14155048 14190094 15140122 16000044 21875158 22712020 25030024 30391023 31765068 46,49 13,09 10,18 15,74 310,95 113,45 62,38 8,73 108,57 181,98 31985047 23,10 61965059 K210012 P36935 10500064 15070063 16500500 16520100 180432 114,49 85,19 182,84 77,17 7,64 471,44 447,24 305,03 368800 41966052 44621G A1048301 A11012 AHZ0202 D22185 G21079 P36935 R25001 V50320 15400-054 15240-062 247,66 92,54 300,17 158,19 176,61 249,88 119,89 249,92 182,84 276,06 408,89 37,66 36,03 3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti). 10 npk Applied Biosystems ABI # 1 TaqMan® MicroRNA Reverse Transcription Kit, 200 reactions 4366596 Power SYBR Green PCR Master Mix (2x5) 4368706 High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 Reactions 4374966 2 3 4 mirVana™ miRNA Isolation Kit AM1560 5 DNA-free™ AM1906 RNAlater® AM7024 Human Placenta Total RNA AM7950 Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) AM9720 Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with included buffer) AM9730 RNaseZap® Wipes AM9786 6 7 8 9 10 Ekvivalents produkts, daudzums, ražotājs, prod. Kods (Interlux piedāvājums) miScript Reverse Transcription Kit (50), 218061, Qiagen (komplektā 4 x 218061 gab. = 200 rekcijas) QuantiTect SYBR Green PCR Kit (200), 204143, Qiagen (komplektā 2 x 204143 gab. = 400 reakcijas) Omniscript RT Kit (200), 205113, Qiagen (kompolektā 1 x 205113 gab. = 200 reakcijas) miRNeasy Mini Kit (50), 217004, Qiagen (komplektā 1 x 217004 gab. = 50 izdalīšanas) RNase-Free DNase Set (50), 79254, Qiagen (komplektā 1 x 79254 gab. = 50 pagatavošanas) RNAlater RNA Stabilization Reagent (250 mL), 76106, Qiagen (komplektā 1 x 76106 gab. = 250 mL) Blue-Ribbon RNA Human Placenta total RNA, 2x50ug, OriGene HT1013 Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1), Sigma Aldrich, 77619100ML Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with included buffer), AppliChem Lifescience A2489.0100 Rnase AWAY wipes (100 gab.) , VWR, 115-1250 Adrona Interlux 110,00 800,00 357,00 500,00 230,00 400,00 229,00e) 200,00 83,00e) 53,00 169,00 190,00 215,00 377,00 68,00 50,00 68,00 29,00 59,00 120,00 e) Piedāvātie produkti ir ekvivalenti, bet nav pilnībā identiski, tāpēc nolemts līgumu slēgt ar abiem pretendentiem. 4. daļa. Mikrofiltrācijas aksesuāri (MILLIPORE vai ekvivalenti). npk 1 Aksesuārs Spin-OUT™ 6,000 Med Columns 0.5ml volume; >10bp cutoff; 10ea Millipore # 2152 Faneks 65,32 Interlux 84,00 11 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Spin-OUT™ 12,000 Med Columns 0.5ml volume; >20bp cutoff; 10ea Montage PCR 250/Pk Millipore Amicon Ultra-4 Centrifugal Filter Unit Amicon Ultra-15, 10 kD, 24 gab/iep. Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk Amicon Ultra-15, 10 kD, 24 gab/iep. Amicon filters Ultra-15, 10kDa Ultracel-10 memb, 96/Pk Amicon Ultra-15, 50 kD, 24 gab/iep. Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk Millipore Amicon Ultra-15 Centrifugal Filter Unit Amicon filters Ultra-15, 100kDa Ultracel-100 memb, 96/Pk Ultrafree-0.5 Biomax 10kDa 100/Pk 16 Ultrafree-0.5 Biomax 10kDa 100/Pk 2156 UFC7PC250 UFC810024/UFC UFC900324 UFC901024 UFC901024 UFC901024 UFC901096 UFC905024 UFC910024 UFC910024 UFC910024/UFC UFC910096 UFV5BGC00 aiztāts ar UFC501096 UFV5BGC00 aiztāts ar UFC501096 17 Ultrafree-0.5 Biomax 100kDa 100/Pk UFV5BHK00 Ultrafree-0.5 Biomax 100kDa 100/Pk UFV5BHK00 18 61,77 220,81 (UFC503096) 116,44 159,04 169,69 169,69 169,69 607,05 159,04 160,46 160,46 160,46 621,96 225,07 80,00 480,00 150,00 215,00 210,00 210,00 210,00 735,00 195,00 205,00 205,00 205,00 750,00 275,00 225,07 275,00 220,81 (UFC510096) 220,81 (UFC510096) 275,00 275,00 12 12. Uzvarētāji, ar kuriem nolemts slēgt vispārējo vienošanos, to piedāvātās preces un cenas: 12.1. SIA Interlux 1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti). npk 1 2 6 7 8 9 10 11 16 18 21 22 23 24 27 28 29 33 35 36 37 Viela Rubidium chloride Tryptone, 1 kg Calcium chloride hexahydrate Copper(II) sulfate pentahydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99-102% Diethyl pyrocarbonate MES monohydrate Sodium chloride, ≥99.5%, BioUltra, for molecular biology, 5kg SDS Trizma base Agarose G-418 disulfate salt, 5g Aluminium oxide Type A-5 Amicase, 1kg Acrylamide/Bis-acrylamide Anti-his antivielas peroksidāzes konjugāts Monoclonal anti-polyHistidine-Peroxidase antibody from mouse BSA; pH ~7 (1% in 0.15 M NaCl), ≥98% (agarose gel electrophoresis), lyophilized powder (Sigma-Aldrich) Brilliant Blue R Boric acid, 1kg Chloramphenicol, 100g Carbenicillin, disodium salt, 89.0-100.5% anhydrous basis Ekvivalents produkts, daudzums, ražotājs, produkta kods (Interlux) Rubidium chloride, 25g, Amresco, 0632-25 Tryptone, 1 kg, BD, 211705 Calcium chloride hexahydrate, 500g, AppliChem Lifescience, A2303.1000 Copper(II) sulfate pentahydrate, pure. p.a., reag. Ph. Eur., 99-101%, 1kg, AppliChem Lifescience, A1367.1000 Diethyl pyrocarbonate, 25ml, Molekula, 11155018 MES monohydrate, 100g, AppliChem Lifescience, A1074.0100 Sodium chloride, ≥99.5%, for molecular biology, 5kg, BDH Prolabo, 443827W SDS, 100g, AppliChem Lifescience, A1112.0100 Trizma base, 1kg, BDH Prolabo, 28808.294 Agarose, 500g, StarLab GmbH, N3101-0500 G-418 disulfate salt, 5g, AppliChem Lifescience, A2167.0001 Aluminium oxide Type A-5, 500g, Sigma, A2039-500G Amicase, 1kg, Sigma, A2427-1KG Acrylamide/Bis-acrylamide, 1L, AppliChem Lifescience, A3658.1000 Anti-his (C-term)antivielas peroksidāzes konjugāts, 1VL, 50 µl, Invitrogen, R930-25 Monoclonal anti-polyHistidine-Peroxidase antibody from mouse,50 µl, Invitrogen, R930-25 BSA; pH ~7, ≥98% (agarose gel electrophoresis), lyophilized powder, 100g, AppliChem Lifescience, A1391.0100 Brilliant Blue R, 50g, AppliChem Lifescience, A1092.0025 Boric acid, 1kg, AppliChem Lifescience, A2940.1000 Chloramphenicol, 100g, AppliChem Lifescience, A7495.0100 Carbenicillin, disodium salt, 89.0-100.5% anhydrous basis, 1g, AppliChem Lifescience, A1491.0001 Cena Ls 69,40 86,20 18,80 23,00 54,70 31,90 29,60 26,20 36,30 164,00 320,40 34,72 112,45 77,18 214,73 214,73 100,00 48,00 24,80 42,20 21,90 13 39 Cesium chloride 40 44 45 51 52 Chamber slides, sterile, 4 wells on glass EDTA, anhydrous, ≥99% (titration) EDTA, 500g Formamide Fetal Bovine Serum Formaldehyde (37%) Solution (Formalin), 500 ml L-Glutathione reduced D-(+)-galactose L-Glutamic acid potassium salt monohydrate Gold(III) chloride trihydrate, 1g D-(+)-Glucose, ≥99% (GC) Glutamine solution Anti-polyHistidine antibody, monoclonal, produced in mouse, 0.2 ml HEPES Imidazole Kanamycin sulfate, 50g LB Broth Leucine MOPS MTT Formazan mitomycin C Magnesium acetate M9 salts 5X 2-Mercaptoethanol Nutrient Mixture F-10 Ham 53 54 55 56 57 59 60 62 63 65 66 67 68 69 70 72 73 74 75 77 80 82 84 89 Potassium phosphate monobasic, 1kg Tween 20 PEG 8000 Parafilm® "M" roll size 2 in. × 250 ft Cesium chloride, 500g, BDH Prolabo, 441574M Chamber slides, sterile, 4 wells on glass, 16gab./iepak., Nunc, 7342049 EDTA, anhydrous, ≥99%, 1kg, AppliChem Lifescience, A5097.1000 EDTA, 500g, AppliChem Lifescience, A1103.0500 Formamide, 500ml, AppliChem Lifescience, A0937.0500 Fetal Bovine Serum, 500ml, Invitrogen, 10106169 Formaldehyde (37%) Solution (Formalin), 500 ml, AppliChem Lifescience, A0877.0500 L-Glutathione reduced, 25g, AppliChem Lifescience, A2084.0025 D-(+)-galactose, 100g, AppliChem Lifescience, A1131.0100 L-Glutamic acid potassium salt monohydrate, 500g, Alfa Aesar, A17232 Gold(III) chloride trihydrate, 1g, Alfa Aesar, 36400 D-(+)-Glucose, ≥99%, 250g, AppliChem Lifescience, A1422.0500 Glutamine solution, 100ml, Invitrogen, 25030024 Anti-polyHistidine antibody, monoclonal, produced in mouse, 50 µl, Invitrogen, R930-25 HEPES, 250g, AppliChem Lifescience, A3268.0250 Imidazole, 100g, AppliChem Lifescience, A1073.0100 Kanamycin sulfate, 50g, AppliChem Lifescience, A1493.0050 LB Broth, 1kg, BD, 1231 Leucine, 250g, AppliChem Lifescience, A3460.0250 MOPS, 100g, BDH Prolabo, 443832T MTT Formazan, 1g, Sigma, M2003-1G Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002 Magnesium acetate, 250g, AppliChem Lifescience, A1877.0250 M9 salts 5X, 250g, Sigma, M6030-250G 2-Mercaptoethanol, 250ml, BDH Prolabo, 436024C Nutrient Mixture F-10 Ham, 6x500ml, Biochrom, F0715 Potassium phosphate monobasic, 1kg, AppliChem Lifescience, A3620.1000 Tween 20, 100ml, BDH Prolabo, 437082Q PEG 8000, 500g, AppliChem LifescienceA2204.0500 Parafilm® "M" roll size 2 in. × 250 ft, 1gab., Brand, 291-1214 377,40 97,82 43,50 20,69 13,70 75,00 29,00 74,20 19,00 55,30 75,40 13,60 8,00 214,73 76,70 15,10 144,50 42,90 49,00 26,20 76,00 204,50 19,90 50,37 14,00 38,70 30,30 9,39 13,60 21,90 14 93 94 96 98 100 103 Ribonuclease A Type I-A Sucrose, for molecular biology, ≥99.5% (GC), 5kg Thiamine hydrochloride Trypsin proteomics grade 108 113 114 115 116 121 122 123 Tryptone Yeast Extract, 1kg Carboy with spigot, polypropylene, 20l, autoclavable Digitonin EGTA HEPES Mitomycin C Mitomycin C ß-Galactosidase Reporter Gene Staning kit X-Gal 124 Magnesium acetate tetrahydrate Ribonuclease A Type I-A, 100mg, AppliChem Lifescience, A2760.0100 Sucrose, for molecular biology, ≥99.5% (GC), 5kg, AppliChem Lifescience, A2211.5000 Thiamine hydrochloride, 250g, AppliChem Lifescience, A0955.0250 Trypsin proteomics grade, 5x20ug, Sigma, T6567-5X20UG Tryptone, 250 g, BD, 211921 Yeast Extract, 1kg, BD, Carboy with spigot, polypropylene, 20l, autoclavable, 1gab., Nunc Nalgene, 216-8762 Digitonin, 500mg, AppliChem Lifescience, A1905.0500 EGTA, 100g, AppliChem Lifescience, A0878.0100 HEPES, 500g, AppliChem Lifescience, A3268.0500 Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002 Mitomycin C, 5x2mg, AppliChem Lifescience, A2190.0002 ß-Galactosidase Reporter Gene Staning kit, 1kit, Sigma, GALS-1KT X-Gal, 100mg, AppliChem Lifescience, A4978.0100 Magnesium acetate tetrahydrate, 1kg, AppliChem Lifescience, A1877.1000 23,60 103,70 54,20 74,00 55,00 96,00 67,80 99,60 163,30 132,90 204,50 204,50 199,04 18,00 50,20 2. daļa. Šūnu inkubēšanas vides un piedevas (INVITROGEN vai ekvivalentas). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 FETAL BOVINE SERUM Recovery Cell Culture Freezing Medium - DMSO DPBS (+)CaCl2 (+) MgCl2 DPBS (-)CaCl2 (-) MgCl2 Versene 1x HEPES 1M Glasgov MEM BHK - 21 L- Glutamine 200MM (100x) DMEM + GlutaMAX Mayer's Hematoxylin MEM Non Essential Amino Acids (100X), liquid Sodium Pyruvate MEM 100 mM, liquid Kanamycin Sulfate LB Broth Base, powder (Lennox L Broth Base)® 10500064 12648010 14040174 14190169 15040033 15630056 21710082 25030024 31966047 008001 11140035 11360039 11815024 12780052 77,17 88,35 89,82 87,17 8,00 47,48 108,12 8,73 104,08 32,98 10,18 6,98 33,17 46,49 15 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Earle's Balanced Salt Solution (EBSS) (1X), liquid Dulbecco's Phosphate Buffered Saline (D-PBS) (1X), liquid Penicillin-Streptomycin, liquid Fetal Bovine Serum, Certified RPMI 1640 Medium (1X), liquid 2-YT Broth L-Glutamine 200 mM (100X), liquid SELECT Agar®, powder F-12 Nutrient Mixture (Ham), liquid Opti-MEM® I Reduced Serum Medium (1X), liquid - with L-Glutamine Dulbecco's Modified Eagle Medium (D-MEM) (1X), liquid (High Glucose) PureLink™ Quick Gel Extraction Kit ProLong® Gold antifade reagent with DAPI - special packaging Foetal bovine serum, heat inactived Penicillin-Streptomycin UltraPure™ Agarose 500 g UltraPure™ Low Melting Point Agarose100 g Primārā antiviela, mouse anti-p16 Primārā antiviela, ERK1/2 [pT202/pY204], Rabbit polyclonal, 100 ul DMEM Primārā antiviela, AKT/PKB [pS473], Rabbit monoclonal, 100 ul Collagen I, Rat Tail, RUO, 5mg/ml Alexa Fluor 594 goat anti—rabbit IgG Primārā antiviela, cdk4, mouse monoclonal, 100 ug DAB histochemistry kit with goat anti-mouse IgG-HRP Goat anti-Rabbit IgG -Alkaline phosphatase, 1 mg ProLong® Gold antifade reagent with DAPI, 5x2ml Zeocin™ Selection Reagent, 1g Vector pTEF1/Zeo Trypsin, 0.5% (10x) with EDTA 4Na, liquid Antibiotic-Antimycotic (100X), liquid 14155048 14190094 15140122 16000044 21875158 22712020 25030024 30391023 31765068 31985047 61965059 K210012 P36935 10500064 15070063 16500500 16520100 180432 368800 41966052 44621G A1048301 A11012 AHZ0202 D22185 G21079 P36935 R25001 V50320 15400-054 15240-062 13,09 10,18 15,74 310,95 113,45 62,38 8,73 108,57 181,98 23,10 114,49 85,19 182,84 77,17 7,64 471,44 447,24 305,03 247,66 92,54 300,17 158,19 176,61 249,88 119,89 249,92 182,84 276,06 408,89 37,66 36,03 3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti). npk Applied Biosystems 4 mirVana™ miRNA Isolation Kit Ekvivalents produkts, daudzums, ražotājs, prod. Kods (Interlux piedāvājums) Cena Ls miRNeasy Mini Kit (50), 217004, Qiagen (komplektā 1 x 217004 gab. = 50 izdalīšanas) 200,00 16 5 DNA-free™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with included buffer) 8 9 RNase-Free DNase Set (50), 79254, Qiagen (komplektā 1 x 79254 gab. = 50 pagatavošanas) Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1), Sigma Aldrich, 77619-100ML Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with included buffer), AppliChem Lifescience A2489.0100 53,00 50,00 29,00 12.2. SIA Labochema Latvija: 1. daļa. Dažāda pielietojuma reaktīvi un aksesuāri (SIGMA vai ekvivalenti). npk 3 4 5 12 13 14 15 17 19 20 25 26 30 Viela o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet cycloheximide Minisart-Plus filters 0.45µm, 50/pack PEG 1000 PEG 200 PEG 300 PEG 400 PEG 4000 Anti-trušu antivielas peroksidāzes konjugāts Agar L-(+)-Arabinose L-(+)-Arabinose, 500g Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat. IgG fraction of antiserum, buffered aqueous solution, 1 ml 31 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit. IgG fraction of antiserum, buffered aqueous solution, 2 ml 32 34 38 41 42 43 46 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat SIGMAFAST BCIP/NTB, 25 tabletes CelLytic™ M NZ-Amine A, 1 kg DMSO DMEM- high glucose Fibronectin solution from human fibroblasts, 100 UL Sigma P6912-100TAB 01810-5G 17829K 81188-250G 88440-250ML-F 90878-250ML-F 91893-250ML-F 95904-250G-F A0545-1ML A1296-100G A3256-100G A3256-500G 200,46 95,58 73,00 13,24 10,73 14,03 12,22 11,01 128,13 43,57 71,79 287,75 A8275 - 1ML 75,12 A9044 - 2ML 114,43 A9169-2ML B5655-25TAB C2978-250ML C7290 D2650-100ML D6429-6X500ML F0556-100UL 135,66 128,38 137,37 82,03 101,65 21,60 124,36 Cena Ls 17 47 F2006-1MG 133,90 48 49 50 52 58 61 64 71 76 78 79 81 83 85 86 87 88 90 91 92 95 97 99 101 102 104 105 106 107 Fibronectin from human plasma cell culture tested, lyophilized powder, 1mg Anti-Fibronectin antibody produced in rabbit. affinity isolated antibody, buffered aqueous solution, 0.2ml Freund`s Adjuvant, incomplete Freund`s Adjuvant, complete Fetal Bovine Serum Sephadex® G-50, Superfine Hepes Iscove's modified dulbecco's medium Nocodazole Mouse monoclonal anti-Nanog IgG1, Clone NNG-811, 25 ul Nitrocellulose membrane for blotting, 0,45mkm, 15x15cm Glass slides coated with poly-L-lysine Pepticase, 250 g Tween 20 PEG 3350 Phosphate buffered saline powder, pH 7.4 Phosphate buffered saline Phosphate-Citrate Buffer Protein A–Peroxidase from Staphylococcus aureus/horseradish, 1 mg RPMI 1640 Medium Retinoic acid Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0% Trypsin-EDTA solution Tryptose Phosphate Broth 1x TRI – Reagent TRI Reagent, 200 ml Plastibrand PD-tips 1.25ml Plastibrand PD-tips 2.5ml Plastibrand PD-tips 5ml Nalgene® vacuum filtration system capacity 150 mL, pore size 0.2 μm, 12 pcs. F3648-.2ML F5506-6X10ML F5881-6X10ML F7524-500ML G5050-50G H0887-100ML I3390-6X500ML M1404-2MG N3038 N8267-5EA P0425-72EA P1192 P1379-1L P3640-500g P3813-10PAK P4417-100TAB P4809-100TAB P8651-1MG R0883-6x500ML R2625-50MG S4641-500G T4049-100ML T8159-100ML T9424-100ML T9424-200ML Z332631-1PAK Z332658-1PAK Z332666-1PAK Z358185-1CS 45,30 77,60 77,23 79,65 132,88 34,14 100,15 20,01 49,74 74,54 36,90 51,48 34,88 15,06 38,82 69,11 156,49 185,95 59,82 17,60 21,75 9,01 10,52 143,23 245,18 40,55 40,55 40,55 63,10 109 110 111 Nalgene® vacuum filtration system capacity 250 mL, pore size 0.2 μm, 12 pcs. Hanna pH standard buffer solution pH 4.01, 500ML Hanna pH standard buffer solution pH 7.01, 500ML Z370606-1CS Z655112-500ML Z655139-500ML 70,80 21,24 21,24 18 112 117 118 109 120 125 Hanna pH standard buffer solution pH 10.01, 500ML Cycloheximide OptiPrep Density Gradient Medium MG-132 Lactacystine Glycine (ReagentPlus®, ≥99% (TLC) grade) Z655155-500ML 01810-5G D1556-250ML C2211-5MG L6785-2MG G7126-1KG 21,24 102,94 198,43 84,85 225,35 39,19 12.3. SIA Adrona: 3. daļa. Reālā laika PCR un RNS izolēšanas reaktīvi un aksesuāri (APPLIED BIOSYSTEMS vai ekvivalenti). npk Applied Biosystems 1 2 3 4 5 6 7 10 TaqMan® MicroRNA Reverse Transcription Kit, 200 reactions Power SYBR Green PCR Master Mix (2x5) High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 Reactions mirVana™ miRNA Isolation Kit DNA-free™ RNAlater® Human Placenta Total RNA RNaseZap® Wipes ABI # Cena Ls 4366596 4368706 4374966 AM1560 AM1906 AM7024 AM7950 AM9786 110,00 357,00 230,00 229,00 83,00 169,00 215,00 59,00 12.4. SIA Faneks: 4. daļa. Mikrofiltrācijas aksesuāri (MILLIPORE vai ekvivalenti). npk 1 2 3 4 5 6 7 8 9 10 Aksesuārs Spin-OUT™ 6,000 Med Columns 0.5ml volume; >10bp cutoff; 10ea Spin-OUT™ 12,000 Med Columns 0.5ml volume; >20bp cutoff; 10ea Montage PCR 250/Pk Millipore Amicon Ultra-4 Centrifugal Filter Unit Amicon Ultra-15, 10 kD, 24 gab/iep. Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk Amicon Ultra-15 10kDa Ultracel-10 memb 24/Pk Amicon Ultra-15, 10 kD, 24 gab/iep. Amicon filters Ultra-15, 10kDa Ultracel-10 memb, 96/Pk Amicon Ultra-15, 50 kD, 24 gab/iep. Millipore # 2152 2156 UFC503096 UFC810024/UFC UFC900324 UFC901024 UFC901024 UFC901024 UFC901096 UFC905024 Cena Ls 65,32 61,77 220,81 116,44 159,04 169,69 169,69 169,69 607,05 159,04 19 11 12 13 14 15 16 17 18 Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk Amicon Ultra-15 100kDa Ultracel-100 memb 24/Pk Millipore Amicon Ultra-15 Centrifugal Filter Unit Amicon filters Ultra-15, 100kDa Ultracel-100 memb, 96/Pk Ultrafree-0.5 Biomax 10kDa 100/Pk Ultrafree-0.5 Biomax 10kDa 100/Pk Ultrafree-0.5 Biomax 100kDa 100/Pk Ultrafree-0.5 Biomax 100kDa 100/Pk UFC910024 UFC910024 UFC910024/UFC UFC910096 UFC501096 UFC501096 UFC510096 UFC510096 160,46 160,46 160,46 621,96 225,07 225,07 220,81 220,81 20 21 12. Pretendenti, ar kuriem nolemts slēgt līgumu: Iepirkuma komisija nolemj slēgt Vispārējās vienošanās līgumu ar Interlux SIA, Labochema Latvija SIA, Faneks SIA un Adrona SIA par atbilstošo preču piegādi. 13. Lēmuma pamatojums: Pretendenti un to piedāvājumi pilnībā atbilst Iepirkumu procedūrā izvirzītajām prasībām un piedāvājumam ir zemākā cena. 14. Valsts pasūtītāja pilnvarotā komisija: 9.1. Latvijas Biomedicīnas pētījumu un studiju centra “NOLIKUMS par iepirkumu Latvijas Biomedicīnas pētījumu un studiju centra vajadzībām” (3.02.2007). Direktora rīkojumi Nr. 7-S/04 (17.08.2004.) un 15-S/08 (18.08.2008). 9.2. Iepirkuma komisijas darbs un tās attiecības ar pretendentu tiek organizētas saskaņā ar “Publisko iepirkumu likumu” un attiecīgajiem MK nolikumiem. Datums: 23. 07. 2010 Valsts pasūtītāja pilnvarotā komisija: Ēriks Jankevics Andris Zeltiņš Svetlana Kazmerika Irēna Čudarāne Pateicamies visiem pretendentiem par piedalīšanos cenu aptaujā. 22