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#: __ Scientist’s Name: ________________ Comp 4d-1 Cell Cycle/Mitosis 4. Investigate, compare, and contrast cell structures, functions, and methods of reproduction. d. Compare and contrast the processes and results of mitosis and meiosis. (DOK 2) DaBook - Section 9 Objectives: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. Discuss the two main parts of cell cycle. Which part is the longest? What % of the cycle? Discuss the events of the three parts of interphase. Define replication. Why is it necessary for chromosomes to duplicate before mitosis begins? What are the two parts of M stage? What is the purpose for mitosis? What might occur if a mistake is made during mitosis? What occurs during cytokinesis? What are the four phases of mitosis (from first to last)? What does each phase of mitosis look like? What happens during each of the phases? What is responsible for moving chromosomes during mitosis? List & explain 2 differences how plant & animal cytokinesis are different. Differentiate chromatin, chromatid, & chromosomes. What is the function of the centromere? What is a cell plate? What are the functions of the cytoplasm & centrioles? How many chromosomes are in a human body cell? What are chromosomes made of? Where are they found? What is a centromere? Draw & label. What are centrioles? What types of cells have them? What is the spindle? What two things limit the size of cells? Why is surface area and volume important for cell sizes? What limits cell division? What is cyclin? Define Cancer. What causes cancer? What are stem cells and where do they come from? 30. Place the following in the correct order and label each phase. 31. Label the following. 2 32. Label each. 33. Label each. 3 Cell Cycle & Division Cell Division: 34. All cells are derived from ___ (Cell Theory) 35. Cell division is ___ 36. Cell division differs in ___ (bacteria) and eukaryotes (protists, fungi, plants, & animals) 37. Some tissues must be repaired often such as the lining of gut, white blood cells, skin cells with a short lifespan 38. Other cells do not divide at all after birth such as ___ & ___ 39. Controlled by proteins called ___ & enzymes that attach to them Reasons for Cell Division: 40. 41. 42. Copying DNA: 43. Since the instructions for making ___ are encoded in the DNA, each new cell must get a complete set of the DNA molecules 44. This requires that the DNA be ___ (replicated, duplicated) before cell division Chromosomes & Their Structure: 45. The plans for making cells are coded in ___ 46. DNA, ___, is a long thin molecule that stores genetic information 47. DNA in a human cell is estimated to consist of six billion pairs of ___ 48. DNA is organized into giant molecules called ___ 49. Chromosomes are made of ___ & visible only when ___ 50. When a cell is not dividing the DNA is less visible & is called ___ 51. DNA in eukaryotic cells wraps tightly around ___ to help pack the DNA during cell division 52. ___ help control the activity of specific DNA genes 53. Proteins bind to ___ and attach chromosome to the ___ in mitosis 54. ___ hold duplicated chromosomes together before they are separated in mitosis 4 55. When DNA makes copies of itself before cell division, each half of the chromosome is ___ 56. DNA of prokaryotes (bacteria) is ___ & is called the ___ Chromosome Numbers: Organism Human Fruit fly Lettuce Goldfish Chromosome Number (2n) 46 8 14 94 57. Humans somatic or ___ cells have 23 pairs of chromosomes or 46 chromosomes (___ or 2n number) 58. The 2 chromatids of a chromosome pair are called ___ (have genes for the same trait at the same location) 59. Human reproductive cells or ___ (sperms & eggs) have one set or 23 chromosomes (___ or n number) 60. Every organism has a specific chromosome number Cell Cycle: 61. Cells go through phases or a ___ during their life before they divide to form new cells 62. The cell cycle includes 2 main parts --63. Cell division includes ___ (nuclear division) and ___ (division of the cytoplasm) 64. ___ is the longest part of a cell's life cycle and is called the "resting stage" because the cell isn't dividing 65. During this phase, cells ___, ___, & ___ 66. Interphase consists of 3 parts --- Interphase: 67. ___ or 1st Growth Phase occurs after a cell has undergone cell division 68. Cells mature & increase in size by making more ___ & ___ while carrying normal metabolic activities during this part of interphase 69. ___ or Synthesis Phase follows G1 and the genetic material of the cell (___) is copied or replicated 70. ___ or 2nd Growth Phase occurs after S Phase and the cell makes all the structures needed to divide 5 Cell Division in Prokaryotes: 71. Prokaryotes such as bacteria do not have a ___ 72. Prokaryotes divide into two identical new cells by the process of ___, an ___ method of reproduction 73. First, the ___, attached to cell membrane, makes a copy of itself and the cell grows to about twice its normal size 74. Next, a ___ forms between the chromosomes & the parent cell ___ (clones) Cell Division in Eukaryotes: 75. Eukaryotes have a ___ & ___ which must be copied exactly so the ___ new cells formed from division will be exactly alike 76. The original ___ cell & 2 new ___ cells must have identical chromosomes 77. DNA is copied in the ___ phase of the cell cycle & organelles, found in the cytoplasm, are copied in the ___ phases 78. Both the ___ (mitosis) and the ___ (cytokinesis) must be divided during cell division in eukaryotes Stages of Mitosis: 79. 80. Mitosis is an ___ method of reproduction 81. Mitosis consists of 4 stages --- ___, ___, ___, & ___ Prophase: 82. Chromosomes become ___ when they condense into ___ 83. ___ attach to each other by the ___ 84. ___ in animal cells move to opposite ends of cell 85. ___ forms from centriole (animals) or microtubules (plants) 86. ___ dissolves 87. ___ disintegrates Metaphase: 88. ___ line up in center or equator of the cell attached to ___ 6 Anaphase: 89. Spindle fibers attached to the ___ pull the ___ apart 90. Chromosomes move ___ of cell Telophase: 91. ___ forms at each end of the cell around the chromosomes 92. ___ reform 93. Chromosomes become less tightly coiled & appear as ___ again 94. ___ begins Cytokinesis: 95. 96. In animals, a groove called the ___ forms pinching the parent cell in two 97. In plants, a ___ forms down the middle of the cell where the new cell wall will be Cancer is Uncontrolled Mitosis: 98. Mitosis must be controlled, otherwise ___ 99. Control is by special ___ produced by specialized genes 100. ___ in control proteins can cause cancer 7 Summary of Mitosis: Interphase 1. 2. Early Prophase 1. 2. 3. Late Prophase Metaphase 1. 2. 3. Anaphase Telophase/Cytokinesis 1. 1. 2. 2. 3. 8 101. Which cell is in metaphase? 102. Cells A and F show an early and a late stage of the same phase of mitosis. What phase is it? 103. In cell A, what structure is labeled X? 104. In cell F, what structure is labeled Y? 105. Which cell is not in a phase of mitosis? 106. What two main changes are taking place in cell B? 107. Sequence the six diagrams in order from first to last. 108. Label each phase. 9 LAB: Mitosis Time Problem How long do onion cells spend in each phase of the cell cycle? Objectives To identify the phases of the cell cycle To determine the length of time cells spend in each phase of cell cycle Materials Picture of onion cell mitosis Protractor Calculator Procedure 1. Using the diagram of onion cell mitosis, identify a single cell in each of the 5 phases of the cell cycle & draw a picture of it in the appropriate space in the table. 2. Count the total number of cells in each phase of the cell cycle & record that number in the table. 3. Calculate the fraction of cells in each phase by dividing the number of cells in that phase by the total number of cells you have counted for all phases. Express your answer as a decimal & then as a percentage. 4. Calculate the number of degrees in a circle graph for each phase by multiplying the fraction of time the cell spends in each phase by 360o. (part/whole x 360) 5. Make a circle graph showing the percentage of time a cell spends in each phase of the cell cycle. Label each portion of the circle with the phase it represents. Data Interphase Prophase Metaphase Anaphase Telophase Appearance Total # of cells in this phase Fraction of cells in this phase Percent of time cells spend in this phase Number of degrees in a circle graph for this phase 10 Results Analysis 1. In which phase of the cell cycle does a cell spend most of its time? 2. In which phase does the cell seem to spend the least time? 3. How does plant cell mitosis differ from animal cell mitosis? 11 12 Mitosis Foldable Directions for making a four-tab book: 1. Fold a sheet of paper in half like a hot dog. 2. Fold this long rectangle in half like a hamburger. 3. Fold both ends back to touch the mountain top or fold it like an accordion. 4. On the side with two valleys and one mountain top, make vertical cuts through one thickness of paper, forming four tabs. Information on foldable: Tab 1 (outside) - Prophase Tab 1 (inside left) - List steps of prophase Tab 1 (inside right) - Draw, color and label prophase Tab 2 (outside) - Metaphase Tab 2 (inside left) - List steps of metaphase Tab 2 (inside right) - Draw, color and label metaphase Tab 3 (outside) - Anaphase Tab 3 (inside left) - List steps of anaphase Tab 3 (inside right) - Draw, color, and label anaphase Tab 4 (outside) - Telophase and Cytokinesis Tab 4 (inside left) - List steps of telophase and cytokinesis (animal and plant). Tab 4 (inside right) - Draw, color and label telophase and cytokinesis 1 2 3 4 13