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TOPICS FOR EXAMINATION II - Biology 1406, CAMPBELL AND REESE 8e KNOW AND UNDERSTAND ALL TOPICS AS A FUNCTION OF THE CONCEPT CHECK HEADINGS IN YOUR TEXT!! CHAPTER 7 Know the macromolecules that are present in the plasma membrane and of which molecules it is composed. What are the major functions of membrane proteins? Know the fluid mosaic model of the plasma membrane thoroughly! Include ECM, cytoskeleton, and carbohydrate components. What is active transport and and how does it occur? What is endocytosis, and what are some examples of it? What is exocytosis? What is the most abundant lipid in the plasma membrane? How are plasma membranes in animal cells anchored and given shape and structure? Which types of molecules move best through the plasma membrane? Which types of molecules pass through a cell membrane most easily? Which types of molecules move the most poorly through the plasma membrane? Know the characteristics of the sodiumpotassium pump. What is the purpose of the glycoproteins which are found on the external surface of the plasma membrane. WHAT IS THE DEFINITION OF DIFFUSION? OF OSMOSIS? How is facilitated diffusion different from diffusion? KNOW AND UNDERSTAND WHAT IS MEANT BY TONICITY, HYPERTONIC, HYPOTONIC, ISOTONIC, TURGID, FLACCID, PLASMOLYSIS, PLASMOLYZED, CRENATION, CRENATED, HEMOLYSIS, HEMOLYZED. KNOW AND UNDERSTAND THE CONDITIONS UNDER WHICH EACH OF THESE WOULD OCCUR IN PLANT AND ANIMAL CELLS. You will also have two questions (4 pts.) concerning the movement of solutes and solvent across a selectively (differentially) permeable membrane. You will be given a pictorial problem for these questions, and will be expected to interpret the movement of solutes and solvent over time. Know that the higher the molarity (M) of a solution, the more solute there is in it, per unit solvent. CHAPTER 8 Know and understand the First and Second Laws of Thermodynamics. What is entropy? What is free energy? How do gradients relate to life? How does equilibrium relate to life? Know all properties possessed by catalysts. Know enzyme structure and function - what is an active site? an allosteric site? What is a substrate? What is a product? How does an enzyme catalyze a reaction? Does an enzyme affect the rate at which a chemical reaction occurs? What is a coenzyme? What is a cofactor? Know and understand the process of feedback inhibition. How is feedback inhibition related to allosteric inhibition? What is energy coupling and why is it important? Thoroughly understand ATP and how it energizes the cellular processes of life. CHAPTER 9 Know and understand the concepts of oxidation and reduction. Know the fermentation pathways of ethanol and lactate. Which cells perform each of these fermentations? Does fermentation include the steps of glycolysis also? Does fermentation generate ATP directly in any other way than by the included steps of glycolysis? What are the end products of lactate fermentation? Starting with the Krebs cycle, (also known as the citric acid cycle), what is the pathway (the sequential steps) that electrons take inside an active mitochondrion? What is the evolutionary significance of glycolysis? Where in the cell does glycolysis take place? What are the chemical properties of NAD+ and NADH regarding oxidation and reduction? Which of these has higher energy electrons? What are the end products of glycolysis? What are the major products of the Krebs cycle? How many ATP molecules are produced from a single molecule of glucose by glycolysis alone? How many are produced if the Krebs cycle is used? What are cristae and why are they so highly folded? WHAT IS THE ELECTRON TRANSPORT SYSTEM AND WHY IS IT IMPORTANT? WHAT IS AEROBIC RESPIRATION? WHAT IS OXIDATIVE PHOSPHORYLATION? BE ABLE TO EXPLAIN THE ELECTRON TRANSPORT SYSTEM AND THE CHEMIOSMOTIC SYNTHESIS OF ATP! What is substrate level phosphorylation? Which are the two molecules that carry energy to the electron transport chain from the Krebs cycle? Where is ATP synthase located in the mitochondria? What is the name of the 2 carbon fragment that enters the Krebs cycle? CHAPTER 10 Know all necessary chemical compounds for photosynthesis. KNOW CYCLIC ELECTRON FLOW (CYCLIC PHOTOPHOSPHORYLATION) AND NON-CYCLIC ELECTRON FLOW (NONCYCLIC PHOTOPHOSPHORYLATION) AND WHAT EACH IS USED FOR. What is P700? P680? Know the optimum absorption wavelengths of P680 and P700. What is NADP+? NADPH? What is the internal structure of the chloroplast? What are thylakoids? What are grana? What are stroma? Is there an electron transport chain in chloroplasts? Where is it found? Do plant cells have mitochondria? Can they also make ATP by oxidative phosphorylation? WHAT IS THE CALVIN CYCLE? BE ABLE TO EXPLAIN THE CALVIN CYCLE AND WHAT IT DOES! What is RuBP? G-3-P? Rubisco? BE ABLE TO EXPLAIN THE LIGHT REACTIONS, THEIR PRODUCTS, AND THE RELATIONSHIP THEY HAVE TO THE CALVIN CYCLE! Know and understand the roles and products of Photosystem II and Photosystem I. Where in the cell does the Calvin cycle take place? WHICH PRODUCTS OF THE CALVIN CYCLE ARE UTILIZED IN THE LIGHT REACTIONS? What is chlorophyll? Be able to deduce the absorption characteristics of a plant's photosynthetic pigment based on the visible color of its leaves. CHAPTER 11 What are the three stages of cell signaling? What are protein kinases? What are ligands? Know the modes of action of G protein signal receptors and tyrosine kinase receptors. Which of these two types of receptors is most important to cell growth and reproduction? What is important about G protein systems? What is signal transduction, how does it work, what is its purpose? Where are cellular signal receptors located? What are the steps of the phosphorylation cascade model? CHAPTER 12 Know and understand all stages of the cell cycle and what happens during each stage. How is the cell cycle regulated? What are kinases - how do they work? What are cyclins and how do they work? How do kinases and cyclins cooperate to promote mitosis? Know and understand all stages of mitosis - what happens during each stage, how daughter cells compare with parent cells as to amount of DNA and numbers of chromosomes before, at, or after any phase, and cytoplasmic contents, chromatid presence or absence, differences between plant cells and animal cells at mitosis and during cytokinesis.