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ANTERIOR / MEDIAL THIGH DISSECTION GUIDE Lower Extremity Lab 1 Mark W. Cornwall, PhD, PT, CPed 1. To remove the skin from the anterior and medial aspects of the thigh, make your first cut from the anterior superior iliac spine (ASIS) along the inguinal ligament to the pubic tubercle. Next make a cut from the midpoint of the inguinal ligament (starting from the first cut described above) to the tibial tubercle. Make your final cut starting from the tibial tubercle, circumferentially, to the lateral aspect of the tibial and then to the medial aspect of the tibial. 2. Once you have completed your incisions, now pull the skin back so that you can see the anterior and medial aspects of the thigh. As you observe the anterior thigh, first dig through the fatty tissue to identify the greater saphenous vein. Note that the greater saphenous vein travels in a distal to proximal direction on the medial aspect of the thigh. Once you have identified the greater saphenous vein, you should then clean all of the fatty tissue away from the fascia late. 3. Now examine the fascia lata, which is the connective tissue layer that covers and binds together the contents of the thigh. Observe the ilio-tibial band that is specialized thickening of the fascia lata. The muscles that insert into the fascia lata are tensor fascia lata and gluteus maximus. 4. Depending on the embalming procedure used, the saphenous opening may still be intact. The saphenous opening serves as the entrance point for the greater saphenous vein to pass from outside the fascia lata to within so that it can connect to the femoral vein. Although they have already been removed, the region of the saphenous opening contains large numbers of lymph nodes. 5. Beginning at the saphenous opening, cut the fascia lata down the midline of the thigh to approximately 6 centimeters above the patella. Using a probe, separate the fascia from the underlying quadriceps muscle group and observe the anterior compartment formed by the fascia lata. 6. At the level of the saphenous opening and just above the patella, cut the fascia perpendicular to the previously made midline cut and remove the fascia to expose the muscles, nerves, and vessels of the anterior and medial thigh. 7. Relocate the saphenous vein at its entrance point into the femoral vein. The femoral vein will not be clearly seen as it is contained in the femoral sheath. The femoral sheath serves as a protective pouch, developed from various abdominal facial layers, that guides the femoral artery and vein from the pelvic cavity into the thigh. 8. The femoral sheath is divided into three compartments. The lateral compartment contains the femoral artery; the middle compartment the femoral vein, and the medial compartment that contains the femoral canal. The femoral canal contains lymph nodes and at the most superior aspect is located the femoral ring. The ring is comprised of dense circular connective tissue that if weakened can allow abdominal contents to enter the canal. This is termed a femoral hernia. Identify the three components of the femoral sheath and then remove and clean the region to expose the femoral artery and vein. 9. The femoral artery bisects the femoral triangle. The femoral triangle is formed by the inguinal ligament (superior border), the sartorius muscle (lateral border), and the adductor longus muscle (medial border). Identify the three borders of the femoral triangle. PT525-Clinical Anatomy I Department of Physical Therapy and Athletic Training 1 10. The first major branch of the femoral artery is the profunda or deep femoral artery. In most cases, the medial and lateral circumflex arteries will branch from the profunda femoral. The medial and lateral circumflex arteries each have 3 and 4 branches, respectively. Both the medial and lateral contribute to the hip joint arterial anastomoses, while the lateral also contributes to the knee complex anastomoses. Identify the lateral and medial circumflex arteries, including as many branches as possible. 11. The femoral nerve enters the thigh just lateral to the femoral sheath. Since the nerve divides into multiple small branches after passing under the inguinal ligament it is technically considered to terminate in the femoral triangle. Two branches of the femoral nerve, the nerve to the vastus medialis and the saphenous nerve (sensory) leave the triangle to travel more distally down the thigh. 12. Now identify and then review the origins, insertions, innervations and actions of the following muscles of the anterior thigh: • Quadriceps a. rectus femoris b. vastus medialis c. vastus lateralis d. vastus intermedius • Sartarius • Iliopsoas (forms lateral floor of femoral) • Tensor Fascia Lata 13. As the femoral artery and vein leave the femoral triangle, they enter the adductor or Hunters’ canal. The adductor canal is formed by the sartorius (medial wall), the adductor longus/magnus (posterior wall) and the vastus medialis (anterior wall). Identify the walls of the adductor canal. NOTE: The sartorius may have to be cut at its midpoint in order to completely expose the canal. Also observe how the femoral artery and vein pass through the hiatus formed by the adductor magnus muscle. After passing through the hiatus, the femoral artery and vein now enter the popliteal fossa and thus, are now called the popliteal artery and vein. 14. Now identify and review the origins, insertions, innervations and actions of the following muscles of the medial thigh: 1) Pectineus (forms medial floor of femoral) 2) Adductor longus 3) Adductor brevis (directly beneath the longus) 4) Adductor magnus 5) Gracilis 14. Identify the anterior branch of obturator nerve by cutting the adductor longus at its midpoint. As you reflect the adductor longus and carefully clean the fascia over the adductor brevis, observe the thin yellow colored cords that are exposed. These cords are the anterior branches of the obturator nerve. PT525-Clinical Anatomy I Department of Physical Therapy and Athletic Training 2 You should be able to identify the following structures on a cadaver or a skeleton. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. Greater saphenous vein Fascia lata Ilio-tibial band Saphenous opening Vastus Medialis Obliqus Vastus Lateralis Obliqus Vastus Intermedialis Rectus Femorus Femoral sheath Lateral compartment Medial compartment Femoral canal Femoral artery Inguinal ligament Sartorius muscle Adductor longus Profunda or deep femoral artery Medial and lateral circumflex arteries Femoral nerve Nerve to the vastus medialis Saphenous nerve Sartarius Iliopsoas Tensor fascia lata Adductor or hunters’ canal. Adductor longus Adductor magnus Hiatus formed by the adductor magnus muscle. Pectineus Gracilis Anterior branch of obturator nerve On the skeleton or other bony materials, identify the following landmarks: a) ASIS b) AIIS c) Pubic tubercle d) Greater Trochanter e) Lesser Trochanter f) Lateral condyle & epicondyle g) Medial condyle & epicondyle h) Adductor tubercle i) Linea aspera j) Patella k) Tibial tubercle (or tuberosity) PT525-Clinical Anatomy I Department of Physical Therapy and Athletic Training 3