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STATION #1 1. Use the outline to take notes on the “Phases of Mitosis” 2. Fill it out on your paper on your folder with a dry erase marker. Then check you answers on the inside. Use your teammates for help. Prophase Brief description (what is happening) Vocabulary (define, drawing can be helpful) Chromatin Sister chromatids Centromere Centrolies Spindle Metaphase Brief description Vocabulary Equator Anaphase Brief description Telophase Brief Description 1. what is the result of mitosis? 2. What type of reproduction is mitosis considered? 3. List two other types of this type of reproduction above. Prophase Brief description (what is happening) -- Nuclear membrane begins to break down -- Chromosomes become visible -- centrioles begin to move to either side of the cell Vocabulary (define, drawing can be helpful) Chromatin – loose form of DNA Sister chromatids - Two identical strands joined by a common centromere as a result of a chromosome that duplicated during the S phase of the cell cycle. Centromere- The most condensed and constricted region of a chromosome, to which the spindle fiber is attached during mitosis. Centrolies- One of two cylindrical cellular structures that are composed of nine triplet microtubules and form the asters during mitosis. Spindle- One of a network of achromatic filaments that extend inward from the poles of a dividing cell, forming a spindle-shaped figure. Metaphase Brief description -- sister chromatids line up at the equator of the cell -- spindle fibers attached at the centromere Vocabulary Equator -- middle Anaphase Brief description --Sister chromatids are pulled apart by spindle fibers --Cell elogates Telophase Brief Description -- nuclear membrane begins to reform -- cell begin to “cleave” animal cells have a cleavage furrow and plants have a cell plate 4. What is the result of mitosis? Two identical daughter cells with exact same DNA 5. What type of reproduction is mitosis considered? Asexual 6. List two other types of this type of reproduction above. Binary Fission Budding STATION #2 1. Observing the Cell cycle in plant and animal cells 2. At this station the different phases of the cell cycle in onion root tips and whitefish blastua cells. You will view five different phases of the cell cycle and make sketches in the space provided. Then check you answer. 3. Neatly label the following structures if visible a. chromosomes b. nuclear membrane c. cell wall d. cleavage furrow e. spindle fibers 4. in the space provided below your drawing identify the phase the cell is going through and the type of cell. A B Phase: _______Metaphase______ Phase: ________Telophase_____ Type of cell: ____animal_______ Type of cell: _____Animal_____ C D Phase: _______Cytokinsesis____ Phase: _______INTERPHASE___ Type of cell: ___Plant_________ Type of cell: ______PLANT_____ E F Phase: ______Prophase_______ Phase: _____Anaphase_______ Type of cell: ________Plant____ Type of cell: ____Animal________ Questions: What is the importance of mitosis to unicellular organisms? growth What is the importance of mitosis to multi-cellular organisms? Growth and repair A B Phase:_____________________ Phase:_____________________ Type of cell:________________ Type of cell:________________ C D Phase:_____________________ Phase:_____________________ Type of cell:________________ Type of cell:________________ E F Phase:_____________________ Phase:_____________________ Type of cell:________________ Type of cell:________________ Questions: What is the importance of mitosis to unicellular organisms? What is the importance of mitosis to multi-cellular organisms? STATION #3 ONLINE ONION ROOT TIP: PHASES OF THE CELL CYCLE For the following activity, go online and view cells undergoing mitosis. You will need to complete the following chart for your portfolio. Procedure: 6. Go to www.biology.arizona.edu. 7. Click on “Cell Biology.” 8. Click on “Online Onion Root Tip: Phases of the Cell Cycle.” 9. Read all of the introductory information and keep clicking NEXT until you reach the Data Table. 10. Identify the correct phase of mitosis as the cells come into view. 11. Fill out the following chart as you go. 12. Answer the questions that follow. ONLINE ONION ROOT TIP: PHASES OF THE CELL CYCLE Interphase # of cells % of cells Prophase Metaphase Anaphase Telophase Total 36 100% Questions: According to your chart, in which phase of the cell cycle do cells spend the most time? According to your chart in which phase of the cell cycle do cells spend the least amount of time? Do these predictions make sense in light of what you already know about the cell cycle? Why or Why not? ONLINE ONION ROOT TIP: PHASES OF THE CELL CYCLE # of cells % of cells Interphase 20 56% Prophase 10 28% Metaphase 3 8% Anaphase 2 5.5% Telophase Total 1 36 2.7% 100% Questions: According to your chart, in which phase of the cell cycle do cells spend the most time? Interphase According to your chart in which phase of the cell cycle do cells spend the least amount of time? Telophase Do these predictions make sense in light of what you already know about the cell cycle? Why or Why not? Yes, STATION #4 THE MEMORY GAME 1. Place all of the cards face down. 2. Flip over two cards. 3. If you have a match (a vocabulary term with the correct definition), you may remove both cards from the board. 4. If you do not have a match, it is the other person’s turn, but you should remember where the cards are so that later in the game you can make a match. 5. Repeat steps 2-4 until all matches have been made. 6. The winner is the person with the most matches. Vocabulary for Memory Game PROPHASE METAPHASE ANAPHASE TELOPHASE INTERPHASE MITOSIS CYTOKINESIS CHROMOSOME CHROMATIN CENTRIOLE CENTROMERE SPINDLE CHROMATID EQUATOR ______First and longest phase of mitosis; chromatin coils into visible chromosomes ______Shortest phase of mitosis; chromosomes move to the equator; chromatids are attached by centromeres ______Centromeres split and chromatid pairs are pulled apart ______Final phase of mitosis; nuclear membrane begins to reappear; new cells prepare for their new independent existence ______Busiest phase of the cell cycle; includes growth and DNA replication ______Division of the nuclear material; two new daughter cells formed that are identical to the mother ______Division of the cytoplasm ______Tangled mass of DNA and proteins; state of DNA during interphase ______Structure that contains DNA; appears right before cellular division and vanishes following cell division ______Structures found in animal cells that duplicate during interphase and move to opposite poles during prophase ______Cell structure that joins two sister chromatids of a chromosome ______Cell structures composed of microtubule fibers; “pull” apart sister chromatids ______Identical halves of a duplicated parent chromosome; held together by a centromere ______Midline of a cell during STATION #5 Cell Cycle Graphics 1. with a partner take the pictures and put them in the correct order following what you know about the cell cycle. 2. This is a quiz grade. When you complete the activity raise your hand and the Instructor will evaluate. 3. If it is completely correct you will receive a 100 4. If it is wrong, for every time the instructor checks your work it is 10 points off. 5. Good Luck Station #6 DNA and Replication 1. Draw the other side to the DNA molecule. 2. Label the following a. Sugar b. Phosphate c. Nitrogen base 1. What type of sugar is found in DNA? 2. What are the four bases to DNA? 3. How many Adenine molecules are in the DNA molecule above? 4. How many Thymines are in the DNA molecule above? 5. What conclusion can you draw from the two questions above? 6. Describe what happens during replication. 7. What is the end result of Replication. 1. What type of sugar is found in DNA? deoxyribose 2. What are the four bases to DNA? A, T, G, C 3. How many Adenine molecules are in the DNA molecule above? five 4. How many Thymines are in the DNA molecule above? five 5. What conclusion can you draw from the two questions above? There are always the same number of Adenines as Thymines because they pair together 6. Describe what happens during replication. Helicase comes in a seperates the two sides of the DNA molecule. Then, DNA polymerase pairs the correct bases with the existing bases creating two DNA molecules 7. What is the end result of Replication. A semi conservative DNA molecule. This means one side is an original side and one side is a new side of each DNA molecule.