Download Micro Lab Unit 1 Flashcards

Lab 5 Microscope Flashcards
How should a microscope be held or carried?
What power should the microscope be set to when you
first begin to look in it?
When should you use the coarse knob on high power?
What focus knob should be used on high power?
What is the function of the Revolving Nosepiece?
What is the platform on which the slide rests?
What secures the slide?
What is the function of the Iris Diaphragm Lever?
What is the function of the Condenser Knob?
What is the function of the adjustment knob?
What is the result of turning the Course Adjustment
Knob?
Why would you use the Fine Adjustment Knob?
What is the only knob to use when using High Power?
With two hands. One supporting the bottom and one
on the arm.
Lowest power
Lowest power
Never!
Fine focus knob
Allows the user to change the magnification.
Stage
Stage Clamp
Allows the user to adjust the contrast by regulating the
amount of light.
To concentrate the light beams on the slide.
Increase or Decrease the light intensity.
The Stage will move up and down.
To focus the image.
The Fine Adjustment Knob.
What is the function of the Slide Movement Knob?
How might you determine the magnification of an
objective lens?
What is the effect of resolution in a microscope?
Moves the slide to the left or right.
Inspect the engraving
What is the effect of contrast in a microscope?
What is the maximum magnification of microscopes
used in most biology laboratories?
What is the maximum resolving power of microscopes
used in most laboratories?
What type of microscopes is used in this laboratory?
To distinguish detail between adjacent objects
1000X
What does parfocal mean?
When the image is in focus on one objective, the
image will be in focus with the other objectives.
Total Magnification = Ocular magnification X
Objective magnification
Scanning (4x)
Low (10x)
High Dry (40x)
Oil Immersion (100x)
10X
10 x 4 = 40
How do you calculate total magnification?
Name four objective lenses used on our microscopes
in lab and their powers.
What is the magnification of the Ocular Lens?
What is the total magnification on the scanning
objective?
What is the total magnification on the low power
objective?
What is the total magnification on the high dry
objective?
The amount of detail that it shows
0.2 μm
Compound, light microscopes
10 x 10 = 100
10 x 40 = 400
Lab 5 Microscope Flashcards
What is the total magnification on the oil immersion
objective?
How do you determine the field of view under high
power?
If the low power field of view diameter is 300 µm,
what is the high dry powers field of view diameter?
How do you determine the size of an object?
Describe how you determine the size of an object
under the microscope.
What is a cell?
What are the two classifications of cells?
Which cell classification is less complex?
What features are characteristic of prokaryotes?
Name one example of a prokaryote
What are the three different morphologies (shapes) of
bacteria?
What are the different types of arrangements of
bacteria?
How do eukaryotic cells differ in size and complexity?
How are eukaryotic cells complex?
Name four types of organisms that are eukaryotic
What structures do plant cells have that animal cells
do not contain?
What structures do animal cells have that plant cells
do not contain?
What is also known as an aquatic green plant?
What is the most observable organelle in elodea?
What is cyclosis?
What is the function of cyclosis?
Besides plants and animals, what are other groups
considered to be Eukaryotes?
10 x 100 = 1000
High dry power diameter = (Low power diameter x
Low power magnification) divided by High power
magnification
(300 x 100) divided by 400
Answer = 30,000/400
75 µm
Size of Object = field diameter for that magnification
divided by # of objects that fit across the diameter
If the field diameter is 300 μm and the object goes
across the diameter of view 3 times, then:
Object size = 300 μm divided by 3
Object size = 100 μm in length
The smallest unit that possesses all seven attributes of
life. It is the basic unit of life.
Prokaryotes and Eukaryotes
Prokaryotes.
lack membrane-bound organelles and lack a nuclear
membrane around their genetic material. Their genetic
material is concentrated into an area called the
nucleoid.
Bacteria
coccus (round), bacillus (rod-shaped) and spirillum
(spiral).
They can exist as singles or
Arranged in a chain (Streptococcus)
Arranged in a cluster (Staphylococcus)
Larger and more complex.
They have a membrane around the genetic material, a
greater quantity of DNA and various membrane bound
organelles
Protists, fungi, plants and animals
A cell wall, chloroplasts, larger vacuoles
centrioles
Elodea
The small chloroplasts
The movement of chloroplasts around the periphery of
each cell.
It helps the plant facilitate transport of materials
throughout the plant
Fungi, amoebas and paramecia. Also kelp, which is
large and multicellular.
Lab 5 Microscope Flashcards
Single-celled, microscopic organisms are what
classification?
What are the 3 subdivisions of protists, based on their
nutritional mode?
What is photosynthesis?
What does heterotrophic mean?
How are eukaryotes and protests similar?
What variety of modes does the protist use to move?
Any cellular organism that
has no nuclear membrane no organelles in the
cytoplasm except ribosomes is a?
any organism having as its fundamental
structural unit a cell type that contains
specialized organelles in the cytoplasm is a?
Image Orientation and Focusing Describe the
orientation of the letter "e" when viewed under the
microscope.
What direction in the field of view did the letter "e"
move when you moved the slide slightly to the right
using the slide movement knobs?
What direction in the field of view did the letter "e"
move when you moved the slide slightly toward you
using the slide movement knobs?
What happened to the light intensity when you
switched from low power to high power?
What can you do to adjust for this change?
What happened to the working distance (distance
between the slide and the objective lens) when you
increased magnification from scanning > low> high
dry?
Calculate the high power diameter using the formula
in the lab write up. Show work.
Convert the high power diameter to um.
Depth of Field What color thread is on top?
What color thread is in the middle?
Which color thread is on the bottom?
Why should you always focus an object on a lower
power before focusing on high power?
Protista (or protist)
Those that are plant-like and can perform
photosynthesis, Those that are animal-like and obtain
energy from internal digesting food, and those that are
fungi-like and break down food sources and then
absorb the nutrients.
produce chemical energy by using sunlight using a
variety of photosynthetic pigments
An animal that eats food
They possess a true nucleus and other membrane
bound organelles
Some may use flagella, cilia, or pseudopodia to move
while some are sessile (do not move).
prokaryote
eukaryote
It is upside down and backwards
It moved to the left
It moved away
Decreased
Open the diaphragm
Decreased
Lab 5 Microscope Flashcards
If your lab partner missed this lab, how would you tell
him/her how to distinguish plant cells from animal
cells when viewed through a microscope?
You are looking at an unknown slide with cells on
them in the lab. List three distinct ways you could tell
whether you were observing a prokaryotic or
eukaryotic cell.
Briefly explain why the onion root did not possess any
chloroplasts.