Download View Dr. Stefano Mattarocci`s Resume / CV

CURRICULUM VITAE
Name:
Stefano Mattarocci
Date and place of birth:
March 1, 1977, Rome, Italy
Nationality:
Italian
Family status
Married
Mailing address:
Rue de Vauche, 5, 1201 Genèva (Switzerland)
Phone
+41 (0)763744288
E-mail
[email protected]
Languages
Italian (mother tongue), English (working proficiency).
CV online
ch.linkedin.com/pub/stefano-mattarocci/4a/a64/448/
+39 3334232771
or
[email protected]
https://www.researchgate.net (Stefano Mattarocci profile)
Education:
2009
PhD in Genetics and Cell Biology, University of Viterbo “La Tuscia”, Italy.
Advisors: Dr. MG Paggi (Department of Therapeutic Programs, Regina Elena Cancer Institute) and
Dr. A. Felsani (Institute of Neurobiology and Molecular Medicine, CNR, EBRI, Rome, Italy).
2007
Professional Habilitation Biologist, University of Viterbo, “La Tuscia”, Italy.
2005
Master’s degree in Biological Sciences (five years), University of Rome “La Sapienza”, Italy.
Grade: summa cum laudae.
Advisor: Dr./Prof. E. D’Ambrosio (Institute of Neurobiology and
Molecular Medicine, CNR, EBRI, Rome, Italy).
Research and employment experience:
2014- present
Senior Post-Doc at the Department of Molecular Biology, Genève University, Switzerland. (PI:
Prof. D.M. Shore).
Research project: the Rif1 N-terminal domain establishes an exclusion zone around junctions
between single-stranded and duplex DNA. (Mattarocci et al., in submission).
Recently, it has been discovered that human Rif1p plays an anti-checkpoint role in DSB (double-strand break) pathway. We
found that S.cerevisae Rif1p is recruited to DSBs and mutations occurring in the motifs interacting with Glc7 (PP1 phosphatase)
affect its anti-checkpoint function. We collaborated with N.Thoma lab (Friedrich Miescher Institute, Basel, Switzerland).
They solved the structure of the Rif1 N-terminal region and demonstrated that this region is able to bind directly to ssDNA.
Moreover, we identified the residues involved in this interaction. Our results raised a two-step model for Rif1 regulating the
checkpoint activation: 1) Rif1 binds directly to DNA through its binding-motifs and is able to outcompete with the activator
protein, RPA, for the ssDNA substrate e 2) it inhibits the massive phosphorylation events that occur during the checkpoint
activation by recruiting Gc7.
2011- 2014
Post-Doc researcher at the Department of Molecular Biology, Genève University, Switzerland.
(PI: Prof. D.M. Shore).
Research project: dissect the molecular mechanism of S.cereviease Rif1p regulating DNA
replication and repair. (Shi et al., 2013; Mattarocci et al., 2014).
Initiation of DNA replication involves several phosphorylation events driven by CDK and DDK kinases. We found that the
S.cerevisae Rif1p (Rap1 Interacting Factor 1, a telomere-binding factor) has an inhibitory effect on the establishment of active
pre-replication complex (pre-RC) by direct interaction with PP1 phosphatase (Glc7 in yeast). The G1 phase-specific Rif1-Glc7
complex is able to counteract the DDK-dependent phosphorylation of the pre-RC activation pathway. These findings elucidate the
mechanism of action of Rif1 in the control of DNA replication and uncover an unanticipated role of PP1 phosphatases in the
regulation of origin firing.
1
2009-2010
Researcher Associate at the Department of Therapeutic Programs, Regina Elena Cancer Institute.
(PI: Dr. M.G. Paggi).
Research project: role of insulin protein in the progression of low-grade human lung carcinomas.
(Mattarocci et al., 2009).
In Dr.Paggi’s lab I studied a possible role of the insulin as a prognostic factor in cancer disease. We found that insulin is present
in non-small cell lung cancer (NSCLC) and its expression correlates with the size and the differentiation state of the cancer.
Moreover, we found that the expression of some proteins involved in the insulin pathway, like insulin receptor and SGK1 kinase,
is altered in NSCLC. All together these data suggest that an intracellular insulin activity is important for the progression of lowgrade human lung carcinomas.
2005- 2009
PhD student researcher at the Department of Therapeutic Programs, Regina Elena Cancer
Institute. (Advisor/PI: Dr. M.G. Paggi).
Research project: Molecular mechanism of HPV-E7 protein inducing the cellular transformation.
(Mileo et al., 2009; Mattarocci et al., 2013).
The E7 Human Papillomavirus (HPV) oncogene forces the infected epithelial cells to proliferate even in the presence of
differentiation or pro-apoptotic factors. We discovered three new E7-cellular interacting proteins, Siva-1, GSTP1 and Gelsolin,
and dissected the molecular mechanisms by which E7 exerts its anti-apoptotic role. We also monitored many cellular factors for
their mRNA expression in HPV-induced cervical lesions and we found a panel of several genes which correlate with the
development of the cervix cancer. These data clearly outline specific pathways, and thus key biological processes, altered in
cervical dysplasia and carcinoma.
2004-2005
Under-graduate researcher (full-time training ship) at the Institute of Neurobiology and
Molecular Medicine, CNR, EBRI, Rome, Italy. (Advisor: Prof. E. D’Ambrosio).
Research project: the role of telomeric 3’-overhang on the regulation and the maintenance of
human telomere structure (Mattarocci et al., 2011; Di Domenico et al., 2013).
During my Master’s Thesis research I investigated a possible role of 3’-overhang, the ssDNA termini of telomeres, inducing
senescence. In cultured primary cell lines, we found that 3’-overhang length is maintained throughout the span-life, but,
interestingly, in some very old subjects where telomeres are very short, it drops down. We pointed out that 3’-overhang is an
important component of telomere structure and that, during ageing, accumulation of both very short telomeres and 3’-overhangs
can lead the-telomeres-dependent activation of senescence.
Moreover, we collaborated with F.Ascenzioni lab (University of La Sapienza, Rome) to analyse the length of 3’-overhang in
budding yeast containing human telomere sequence (HY). We found that these telomeres have very long 3’-overhang and an
accumulation of the specific ssDNA-binding protein Rad51. We raise the possibility that Rad51 may outcompete with telomerase
for the binding of these long 3’-overhang favouring the recombination-dependent telomere maintenance.
Techniques:
Cell biology: Immortalized and primary human cell colture. Transient and stable transfections. Viral infection.
siRNA technique. Yeast transformation. Tagging and deletion of endogenous genes. Synchronization of cultures.
Molecular biology: Telomeric-Oligonucleotide Ligation Assay. Polymerase Chain Reaction (PCR, RT-PCR, RealTime PCR, low density array by ABI 7900HT and by Roche LightCycler 480II). Chromosomes “in situ”
hybridization (FISH). Immunohistochemistry. ELISA assay. DNA fingerprinting for forensic analysis. Co- and
Chromatin- immunoprecipitation (Co-IP and ChIP). Cloning. Site-directed and random mutagenesis.
2
International peer-reviewed publications: (h-index: 8)
1) In preparation: Mattarocci S, Shi T, Shyian M, Reinert J, Bunker RD, Faty M, Thoma N, Shore DM. The Rif1
N-terminal domain establishes an exclusion zone around junctions between single-stranded and duplex DNA,
Science.
2) Mattarocci S*, Shyian M*, Lemmens L, Altintas D., Shi T, Thoma N, Damay P, Hardy C, Shore DM. Rif1
controls DNA replication timing in yeast through the PP1 phosphatase Glc7. Cell Rep., 2014;7(1):62-9. 2014.
IF: 7.22
3) Mattarocci S, Abbruzzese C, Mileo AM, Carosi M, Pescarmona E, Vico C, Federico A, Vizza E, Corrado G,
Arisi I, Felsani A, Paggi MG. Identification of pivotal cellular factors involved in HPV-induced dysplastic and
neoplastic cervical pathologies. J Cell Physiol. 2014, 229(4):463-70. IF: 4.22
4) Shi T, Bunker RD*, Mattarocci S*, Ribeyre C, Faty M, Gut H, Scrima A, Rass U, Rubin SM, Shore D, Thomä
NH. Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions. Cell. 2013;
153(6):1340-53. IF: 31.96
*equal contribution
5) Mileo AM, Abbruzzese C, Vico C, Bellacchio E, Matarrese P, Ascione B, Federico A, Della Bianca S,
Mattarocci S, Malorni W, Paggi MG. The human papillomavirus-16 E7 oncoprotein exerts antiapoptotic effects
via its physical interaction with the actin-binding protein gelsolin. Carcinogenesis. 2013; 34(10):2424-33.
IF: 5.64
6) Di Domenico EG, Mattarocci S, Cimino-Reale G, Parisi P, Cifani N, D'Ambrosio E, Zakian VA, Ascenzioni F.
Tel1 and Rad51 are involved in the maintenance of telomeres with capping deficiency. Nucleic Acids Res. 2013;
41(13):6490-500. IF: 8.28
7) Akl EA, Meerpohl JJ, Raad D, Piaggio G, Mattioni M, Paggi MG, Gurtner A, Mattarocci S, Tahir R, Muti P,
Schünemann HJ. Effects of assessing the productivity of faculty in academic medical centres: a systematic review.
CMAJ. 2012; 184(11):E602-12. IF:6.47
8) Abbruzzese C, Mattarocci S, Pizzuti L, Mileo AM, Visca P, Antoniani B, Alessandrini G, Facciolo F, Amato R,
D'Antona L, Rinaldi M, Felsani A, Perrotti N, Paggi MG. Determination of SGK1 mRNA in non-small cell lung
cancer samples underlines high expression in squamous cell carcinomas. J Exp Clin Cancer Res. 2012; 31:4.
IF: 3.07
9) Mattarocci S, D'Ambrosio E, Tafaro L, Somma V, Zannino G, Marigliano V, Ascenzioni F, Cimino-Reale G.
Erosion of telomeric 3'-overhangs in white blood cells of aged subjects with high frequency of very short
telomeres. Mech Ageing Dev. 2011; 132(1-2):27-32. IF: 4.18
10) Mileo AM, Abbruzzese C, Mattarocci S, Bellacchio E, Pisano P, Federico A, Maresca V, Picardo M, Giorgi A,
Maras B, Schininà ME, Paggi MG. Human papillomavirus-16 E7 interacts with glutathione S-transferase P1 and
enhances its role in cell survival. PLos One. 2009; 4(10):e7254. IF:3.73
11) Amato R, D'Antona L, Porciatti G, Agosti V, Menniti M, Rinaldo C, Costa N, Bellacchio E, Mattarocci S,
Fuiano G, Soddu S, Paggi MG, Lang F, Perrotti N. Sgk1 activates MDM2-dependent p53 degradation and affects
cell proliferation, survival, and differentiation. J Mol Med. 2009; 87(12):1221-39. IF: 4.77
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12) Mattarocci S, Abbruzzese C, Mileo AM, Visca P, Antoniani B, Alessandrini G, Facciolo F, Felsani A,
Radulescu RT, Paggi MG. Intracellular presence of insulin and its phosphorylated receptor in non-small cell lung
cancer. J Cell Physiol. 2009; 221(3):766-70. IF: 4.22
13) Fierabracci A, Puglisi MA, Giuliani L, Mattarocci S, Gallinella-Muzi M. Identification of an adult
stem/progenitor cell-like population in the human thyroid. J Endocrinol. 2008; 198(3):471-87. IF: 4.06
14) Severino A, Abbruzzese C, Manente L, Valderas AA, Mattarocci S, Federico A, Starace G, Chersi A, Mileo
AM, Paggi MG. Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human
immortalized keratinocytes. J Cell Physiol. 2007; 212(1):118-25. IF: 4.22
International conference proceedings:
1) Lemmens L, Mattarocci S, Marcomini I, Shi T, Follonier C, Lopes M, Thomä N, Shore D. Global control of
DNA replication timing by the budding yeast telomere protein Rif1 Epigenetics & Chromatin 2013, 6(Suppl
1):P99 (poster presentation). 18 March 2013.
2) Shi T, Bunker RD, Ribeyre C, Mattarocci S, Faty M, Gut H, Scrima A, Rass U, Shore D, Thoma N. Rif1 and
Rif2 shape telomere function and architecture through multivalent Rap1interactions. 5th EMBO congress on
Telomeres and the DNA Damage Response. Isle de la Sourge, France, October 04-07, 2012 (poster
presentation).
3)
Mattarocci S, Somma V, Pascale E, Ascensioni F, D’Ambrosio E, Cimino Reale G. The length of 3’-overhang
measured in vitro and in vivo aging cells. 4th EMBO congress on Telomeres and the DNA Damage Response.
Marseille, France, September 14-17, 2010 (poster presentation).
4)
Di Domenico EG, Mattarocci S, Somma V, D’Ambrosio E, Zakian VA, Ascensioni F. G-tail and telomere
length in Humanized Yeast. 4th EMBO congress on Telomeres and the DNA Damage Response. Marseille,
France, September 14-17, 2010 (poster presentation).
5)
Mileo AM, Giorgi A, Bellacchio E, Abruzzese C, Mattarocci S, Pisano P, Federico A, Maresca V, Picardo M,
Maras B, Schininà ME, Paggi MG. Human Papillomavirus-16 E7 interacts with glutathione S-transferase P1
and enhances its role in cell survival. Translational cancer medicine, Jerusalem, Israel, November 3-6, 2008
(poster presentation)
6)
D'Ambrosio E, Cimino Reale G, Mattarocci S, Pascale E, Tafaro L, Zannino G, Marigliano V. Erosion of the
telomeric 3'-overhang in old age. 2005 ASBMB Annual Meeting, San Diego, California, April 2-6, 2005 (poster
presentation).
Plus other 10 Congress Proceeding
Genèva, 2015.
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