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Photosynthesis Plants, unlike animals, produce their own food. They are capable of taking light energy from the Sun and use it to change (convert) readily available chemicals into energy rich compounds that they can use as food. Water from the soil and carbon dioxide from the air are combined to form glucose. The light energy needed for this reaction to occur is captured by a green pigment in the leaves of the plant called chlorophyll. The whole process is called photosynthesis. Light energy from the Sun is essential for photosynthesis. The glucose made in this process is important as it can be broken down to provide energy or it can be converted into more complex substances such as starch, fats or protein. Reactions which take in energy, such as this one, are endothermic reactions and photosynthesis is the most important endothermic reaction on Earth!! A starch molecule is made up (consists) of many, many glucose molecules bonded together in a long chain. The long chain curls up on itself to form a very compact molecule that looks a bit like a spring. Glucose molecules are very small, soluble and reactive. If there is more than enough (excess) glucose in the leaf, it is turned into starch. Starch is insoluble and very compact and so is an ideal way of storing glucose. The starch forms macroscopic grains that can be seen in the cells. This store of glucose can be broken down and used when energy is needed. Testing a Leaf for Starch. This test is used to determine if a leaf has been photosynthesising. If starch is present in the leaf it will go blue-black in colour when iodine solution is added. The Procedure Why boil the leaf? Why place the boiled leaf in ethanol? Why rinse the boiled leaf again in the hot water? Factors that affect the Rate of Photosynthesis What are the factors that affect how fast photosynthesis occurs in plants? What happens if one of these factors changes? What type of variable does that factor become – dependent or independent? Which axis of a graph is the dependent variable recorded? Light Below are some results from an experiment carried out using a lamp at varying distances from a water plant called Elodea. Distance of lamp from Elodea (cm) Number of Bubbles (mins) 100 80 60 40 20 0 13 12 31 41 55 76 Plot a graph of these results Analyse the graph. What does it tell you about the results? What can you conclude? When the light is very close to the Elodea the rate of photosynthesis increases a lot. What other factor, apart from light from the lamp, could explain this big increase? Look at the graph below. What does line A tell you about the effect of light intensity on the rate of photosynthesis? Why does the graph level out? What do lines B and C show? Analysing these results should show that the rate of photosynthesis is controlled by many factors. The actual rate is determined by the factor in shortest supply (the one that there is least of). This factor is called the LIMITING FACTOR. In summer, when there is plenty of bright light, carbon dioxide (CO2) is usually a limiting factor. In winter, light is often a limiting factor.