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Microscope Notes Name: One of the major developments in the field of biology was the invention of the _____________________. This device provides an ____________ image of a small object, an object otherwise too small to be seen with an unaided eye. One of the first microscopes was made by ____ ______________________________. This microscope only had one lens mounted to a metal support and is known as a _____________________ microscope. Its total magnification was about 300X. A short time later, scientists began using the ______________________ microscope, which is composed of ________ lenses. The strongest of the compound microscopes is about ___________________ times. The limitation of the compound microscope is its _____________________ power. The resolving power is the ability of an instrument to separate and distinguish two objects. Example: Look at the two lines on the side of your notes. When you are close to them, you can easily tell that they are separate. When you are far from them, they appear to be one line. At this point, your eye can no longer resolve the two lines. The problem of the resolving power of the compound microscope was solved in the 1940’s when the _____________________ microscope was invented. This method uses a beam of electrons rather than light. The limitation of microscope is that it cannot be used to study living specimens. The organisms must be sliced very thinly and fixed with toxic chemicals. The most recently developed microscope is the __________________ ________________ microscope. Specimens do not have to be sliced thin for viewing so living organisms may be observed. In the images below, the first is a virus viewed with an electron microscope. The second is a tick’s mouth parts taken with a scanning electron microscope. The diagram above illustrates the capabilities and limitations of these microscopes. Below is a diagram of a compound microscope similar to those that we will be using in class. 1. 2. a. b. c. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Modern Cell Theory All living things are made up of _____________. Organisms containing only one cell are called __________, while organisms that are made up of many cells are called _________________. You are _______________ because you are made up of _____________ of cells. Cells were first examined by __________________ in 1665 when he observed cork under a microscope. Understanding of the cell increased with the discoveries of the following scientists: _______________________:discovered the nucleus (1831). ______________________ :stated that the cell is the unit of structure in plants (1838). ________________________:cell is the unit of structure in animals (1839). ________________________:all cells come from preexisting cells (1859). The Modern Cell Theory: *know this* 1. 2. 3. Cell Structures and Functions Notes Cells differ in 1. _____________________________________ 2. _____________________________________ 3. _____________________________________ Internal Organization _______________: contains _______ which directs the activity of the cell ________________: a cell component that performs specific functions in the cell _________________: cells that contain a nucleus and membrane-bound organelles _________________: cells that lack nuclei and membrane-bound organelles Eukaryotes (______________, _____________, ___________, ____________) and prokaryotes (______________) differ greatly in structure. Prokaryotes Eukaryotes 1 - 10 μm 10 - 50 μm billion years ago billion years ago nucleus? membrane-bound organelles? size when evolved? cytoplasm? cell membrane? cell wall? Some Do ribosomes? DNA? examples Circular Free Floating Cells can differ greatly from one another in size and shape. In multicellular organisms, there is tremendous diversity among cells because they are specialized to perform certain functions. There is a strong correlation between shape and function. Cells are built a certain way in order to perform their particular function more efficiently. The organelles inside a cell dictate what the job of the cell will be. Each living cell carries out the tasks of __________________, transforming food into ____________, getting rid of _____________, and ____________________. Cell Part or Organelle Description and functions the outermost living ___________ of animal cells. ______________ the exchange of substances between the __________ and its environment. a ______________, non-.living, rigid structure that is produced by some cells; found outside of the cell membrane; ________________ and ___________________ the cell; not present in ____________________. thick __________ filling cell’s interior; location of most metabolic activity; contains the ____________________ a network of __________ constructed of microtubules and microfilaments; __________ cell and maintains _______________. membrane-bound; controls _______ activities such as _____________. outer boundary of _______________; consists of double membrane containing ___________. the ___________ material of the cell; in nondividing cells they appear as thin, threadlike strands called _______________________. spherical structure involved in the synthesis and assembly of _______________; one or more may appear in nucleus the fluid-filled interior of the _______ in which the other parts are suspended a system of membranous tubules that penetrates through out the cytoplasm & is continuous with the nuclear envelope. Intercellular highway (a path along which molecules move from one part of the cell to another) has ___________ attached to it; helps to store and transport _______ lacks _______________; functions in metabolic processes including synthesis, storage, and transport of ______ and carbohydrates w/in cell. tiny particles floating freely in cytoplasm or attached to ER; NOT membrane-bound; aggregates of protein and RNA molecules; location of ___________________synthesis; bacteria cell _________________ human liver cell _______________ stacks of flattened membranous sacs surrounded by transport vesicles; receives proteins from the rER and distributes them to other organelles or out of the cell (receiving, processing, packaging, and shipping) large oval organelles; double membrane; inner membrane is _______________ which is folded to provide increased surface area; involved in production of ________ as cell ______________ occurs here. membrane-bound vesicles; formed by pinching off Golgi apparatus; contain _______________ enzymes; digests _______ molecules, invading objects, or worn out cell ________ ________________ cells only use ___________ to break down fats (these molecules then go to the mitochondria as fuel for respiration); when in the liver, they detoxify alcohol and other harmful cmpds; Degrade hydrogen peroxide, a toxic compound that can be produced during metabolism, into __________. large membrane sacs used as _____________ chambers within the cytoplasm; contain ________ molecules the cell has engulfed or stored molecules such as lipids, pigments, or wastes; Animal cells: they are ___________ and small Plant cells: ________________: one single large vacuole taking up to _____% available space; contains mostly ________ & provides internal support paired cylinders of microtubules @ ____________ angles; usually near the ______________; involved in cell division in ___________________ cells found in _______; contain pigments colorless plastid; store __________ contain ________________ pigments that give _____________, flowers, and autumn leaves their hues green organelles found in plants & other photosynthetic organisms; double membrane; interior contains ______________ membranes where the pigment ____________ is found; chlorophyll is essential for photosynthesis hair-like organelles that extend from the surface of cells –When they are present in large numbers on a cell they are called _________ ex: ___________________ –When they are less numerous and longer they are called ___________ex. __________ & algae for cell motility & to move ____________over surface Animal cells are very similar to plant cells except for the following major differences: –Animal cells do not contain _____________________ –Animal cells are not surrounded by _______________________ –The vacuoles in ______________ are much larger than those of _________________ Animal cell Plant Cell Aerobic Respiration / Photosynthesis Comparison Chart Aerobic Respiration Where it happens in the cell Explanation of Process Chemical Equation Photosynthesis Molecule movement and Cells _______________________: All organisms encounter great fluctuations in their environments (change in ______________, ______________, ________________, etc) that cause them to struggle continuously to maintain a steady state. The process of maintaining a relatively constant internal environment despite changing external conditions is called __________________________. If a cell is to maintain its identity and remain alive, it must maintain an internal environment that is __________________ from its surroundings. The __________ __________________ separates the chemicals in the cell from those in its environment. The plasma membrane can be described according to its ________________________________: 1. __________________________: the membrane is impermeable when a substance cannot pass through. 2. __________________________: the membrane is permeable when a substance can pass through. 3. __________________________ (semi-permeable): the membrane allows some substances through while others cannot. Moving Materials Into and Out of Cells: Particles move into or out of a cell by passing through the cell’s _________ _________________. _______________ transport is the movement of a substance through a cell’s membrane ______________ the cell using any ______________. There are several different types of passive transport: 1. ______________________: A net movement of particles from an area of _____________ concentration to an area of _______________ concentration. In the example below, a drop of ink diffuses until it fills the volume uniformly. At this point the system can be described as being in ______________________. Equilibrium means that the _______________ of a substance is the same throughout a __________. Equilibrium Key terms: a) ___________: a substance that _______________ in another. Examples: sugars, amino acids, and ions are solutes in cells. b) _____________: the more plentiful substance that dissolves the solute; in cells, the solvent is ________________. c) ______________: the mixture of solutes and solvent. d) ______________________________: the difference in concentration of a substance across a space. 2. The second type of passive transport is __________. Osmosis is simply the diffusion of water molecules through a ___________ from an area of __________ concentration to an area of ________ concentration. We are comparing the concentrations of two solutions. ___________ is how the concentrations of solutes in two solutions compare; determines movement of water into or out of cells. The comparisons fall into one of three categories: A. _______________ solution = solute concentration inside and outside cells is _________. There is ___ net movement of water into or out of cell. ___% water in cell : ___% water out of cell B. ______________ (low) solution = low in solutes (high in water molecules). Therefore water moves _______ cell. ______% water in cell (______): _______% water outside cell (________) C. ________________ (high) solution = high in solutes (low in water molecules). Therefore water moves ________ cell. _______% water in cell (_______): ____% water outside cell (________) In the example below, side B has a higher concentration of water molecules to side A (side B is hypotonic, side A is hypertonic). Water will move from side B to side A. Before After Above is an example of a human blood cell in the 3 types of solution. Note the cell is maintaining homeostasis in the isotonic solution. The cell gains water in the _____________ solution until it has __________ or ruptured. The cell __________ in the ____________ solution because it loses water. This second example demonstrates the effect of osmosis on plant cells. In the first picture, plant cells have been placed in a ___________ solution so that water has entered the cells. The _____________ keep the cells from rupturing. The pressure within the cell (_________ _________) is great and the cell is said to be ___________. The second picture illustrates a plant cell in _____________ solution. _____________ has occurred and the plant is ___________ (low _______________ pressure). 3. The third type of passive transport is ______________ diffusion. In this type of transport, some molecules such as _____________ is transported across the membrane from _________ conc. to __________ conc. by a _________________. The protein is specific in the molecule it can carry. __________________: ________________ is required to move a material across the cell membrane ________________ a concentration difference (low to high concentration). These are the types of active transport: 1) _______________ pumps: cause the production of ____________ molecules (the form of energy used by a cell); these pumps transport protons through the internal plasma membranes of the mitochondria and chloroplasts. 2) ____________________ Pumps and couple channels: Uses the energy of ___________ to power the active transport of sodium (Na+) ions out through a cell’s membrane and potassium (K) in. This is the most important energy-using process in your body!!!!! More than 1/3 of all the energy used by a human cell (that is not dividing) is used to transport sodium ions. Why? 1) This is how nerve cells send impulses (more later)... and.... 2) They help to transport _________ particles and a variety of other particles into your cells by two different kinds of channels. One is the sodium-potassium pump. The other channels are coupled channels. How? 1. Active transport of Na+ out of the cell increases the Na+ concentration outside the cell (as many as 300 per sec). This increase leads to step 2. 2. Na+ move back into the cell by the coupled channels that also carry sugar molecules. The coupled channel contains two passageways through the membrane. The force of the Na+ is so great that it pulls sugar molecules into the cell too. How Molecules Cross the Membrane Active/passive Molecules that move Diffusion direction Energy needed Protein needed Osmosis Facilitated diffusion Active transport Sometimes food particles are too large to enter through protein channels. In this case, the cell engulfs the particle by means of ______________. If the material is liquid and contains dissolved molecules, the endocytosis is called _______________. Human egg cells receive nourishment via __________________. If the material brought into the cell is another cell or other fragment of organic matter, the process is called ________________________. Ex: white blood cells, Amoebas. phagocytosis pinocytosis Phagocytosis & the Amoeba _________________ is the opposite of endocytosis. This is when the cell ___________________________ waste materials out of the cell. The Cell Membrane Notes Cell Membrane Structure The _________________________________________________ of the cell membrane shows that it is composed of proteins that are similar to shifting tiles. The spaces between the tiles are filled with fluid-like ________________________________. Phospholipids consist of ___________________________ (water-loving) heads, which point towards the outside environment and the cytoplasm. The _____________________________________________ repel the water and point in. Extracellular fluid (watery environment) Phospholipid bilayer Hydrophobic tails Hydrophilic heads cytoplasm (watery environment) This causes the phospholipids to form a double layer (bilayer) that acts like a barrier between the cell and the environment. The ___________________________________ also contains _____________________________, which makes it stronger, more flexible and more permeable. Memorize Fig. 7-12 on p. 182 of textbook!!! The purpose of the membrane is to control what goes in and out of the cell!