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Hull's School Ltd Cell Cycle and Mitosis Exam Questions Topic 3.2 59 marks 1. Read through the following account of mitosis in an animal cell, then write on the dotted lines the most appropriate word or words to complete the account. During prophase, the ................................................ breaks down and the ................................................ migrate to opposite poles of the cell. During ................................................, the chromosomes are visible as pairs of chromatids, lined up along the equator of the cell. Spindle fibres extend from the poles of the cell and attach to the ................................................ . In ................................................, the spindle fibres contract, pulling the chromatids apart. (Total 5 marks) 2. The diagram below shows the structure of a chromosome as it might appear at the end of prophase of mitosis. A (a) B Name the parts labelled A and B. A ................................................................................................................................. B ................................................................................................................................. (2) Hull's School Ltd 1 (b) During metaphase of mitosis, the chromosomes become attached to the equator of the spindle. Name the stage of mitosis that follows metaphase and describe the events that occur in this stage. Stage ........................................................................................................................... Events occurring ........................................................................................................ .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (3) (c) Explain the significance of the stage you have named and described in (b). .................................................................................................................................... .................................................................................................................................... (1) (d) Mitosis forms part of the cell cycle. Name one other stage of the cell cycle and state what occurs in the stage that you have named. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (Total 8 marks) 3. (a) The table below describes some of the key events that occur during mitosis Complete the table by writing the name of the stage of mitosis next to its description. Key events Stage Chromatids separate and move to opposite poles of the dividing cell. Chromosomes shorten and thicken. The nuclear envelope breaks down and the spindle forms. The spindle fibres break down, the nuclear membrane re-forms and the chromosomes elongate. Chromosomes line up on the equator of the cell, attached to spindle fibres by their centromeres. (4) Hull's School Ltd 2 (b) The graph below illustrates the change in DNA content during the cell cycle Mass of DNA / arbitrary units 6 C 5 D 4 3 A B 2 G1 1 0 0 2 4 6 8 10 12 14 16 18 20 22 26 24 Time / hours (i) Calculate the percentage of the cell cycle time spent in G1. Answer................................................................... (3) (ii) At which point, A, B, C or D, does chromosome replication (the S phase) begin? Explain your answer. .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... (2) (Total 9 marks) Hull's School Ltd 3 4. The drawing below shows an animal cell, magnified × 1000, undergoing mitosis. (a) Give two features that help to identify this as an animal cell. 1 ................................................................................................................................. 2 ................................................................................................................................. (2) (b) Name the parts labelled A and B. A ................................................................................................................................ B ................................................................................................................................ (2) (c) Name the stage of mitosis shown in the drawing. .................................................................................................................................... (1) (d) Calculate the actual maximum diameter of this cell. Show your working. Answer ........................................................... (2) (Total 7 marks) Hull's School Ltd 4 5. The diagram below shows cells from a root tip, prepared by the root tip squash method. (a) Describe how you would prepare a root tip squash so that mitosis can be studied. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. ……………………………………………………………………………………….. (4) (b) State which of the cells labelled A–E is in: (i) metaphase ................... (ii) anaphase ..................... (2) (c) State two events that take place during interphase. 1. …………………………………………………………………………………….. 2. …………………………………………………………………………………….. (2) (Total 8 marks) Hull's School Ltd 5 6. The diagrams below show four stages of mitosis. (a) (i) A B C D Write the letters of the stages in the sequence in which they occur during mitosis. ........... .......... .......... ........... (1) (ii) Name stage D. ................................................................................................................................. (1) Hull's School Ltd 6 (b) The graph below shows how the quantity of DNA varies with time in a cell cycle. 4 Quantity of DNA / arbitrary units 3 2 1 0 0 5 10 15 20 25 Time/hours (i) Explain the changes in the quantity of DNA that take place: between 10 to 15 hours ..................................................................................................................................... ..................................................................................................................................... at 20 hours ..................................................................................................................................... ..................................................................................................................................... (2) (ii) What is happening- in the cell between 15 and 20 hours? ..................................................................................................................................... ..................................................................................................................................... (1) (iii) What is the minimum length of time that interphase would occupy in this cell cycle? ..................................................................................................................................... (1) (Total 6 marks) Hull's School Ltd 7 7. The diagrams below show cells from a garlic plant at five different stages of mitosis as seen using a light microscope. These stages are in the wrong order. A (a) B C D E Give the correct order in which the five stages take place by using the letters A–E. 1st stage ............ 2nd stage ............ 4th stage ............ 5th stage ............ 3rd stage ............ (2) (b) (i) Name the part of a garlic plant which is used to prepare a slide showing the stages of mitosis. ........................................................................................................................... (1) (ii) Name a stain which is used to make the chromosomes easy to see. ........................................................................................................................... (1) (c) Name the process which must occur in the genetic material before the chromosomes become visible. (i) Name of the process ......................................................................................... (1) (ii) State what happens to the genetic material during this process. ........................................................................................................................... ........................................................................................................................... (1) (Total 6 marks) Hull's School Ltd 8 8. (a) Give an account of the events that take place during prophase and metaphase of mitosis. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (5) (Total 5 marks) Hull's School Ltd 9 9. The graphs below show changes in the DNA content of cells during the cell cycle in two different plants, A and B. Plant A DNA content in each cell / arbitrary units 6 3 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 Time / hours Plant B DNA content in each cell / arbitrary units 6 3 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 Time / hours (a) Compare the cell cycle of plant A with the cell cycle of plant B. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) Hull's School Ltd 10 (b) Describe the events that are occurring inside the cells of plant A between 11 and 13 hours. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (Total 5 marks) Hull's School Ltd 11