Download DNA Technology Worksheet

DNA Technology
NAME:________________________
1. Name two situations when DNA fingerprints are useful.
_________________________________ and _______________________________
2. How does the DNA migrate from one end of the gel to the other?
3. What cuts up the DNA into tiny fragments? _____________________________________
Use the gels below to answer the following questions.
felon
baby
mom
dad
1
dad
2
felon
S1
S2
mom
child
1
child
2
S2
S3
S3
6. Suzy was assaulted in
an alley. The police
collected a sample of
cells that was left at the
crime scene and now
have 3 suspects in
custody. Which of the
suspects assaulted Suzy?
4. Mrs. Smith has a baby
named Jessica. She
believes one of two men
can be the father of her
child. A paternity test is
done and the results are
shown above. Which of
the 2 men are baby
Jessica’s father?
dad
S1
5. Lt. Russ is investigating a
murder scene. The felon was
scratched by his victim & some
of his skin cells were found
under the victim’s fingernails.
A DNA test was performed.
Which of the suspects is the
murderer?
child
3
child
4
child
5
child
6
7. Mr. & Mrs. Jones just gave birth to
fraternal twins- Bob and Jane.
Unfortunately, the nurse has confused the
Jones twins with 4 other babies. The doctors
took samples of DNA from each of the
babies and Mr. & Mrs. Jones. Which of the
6 children are Mr. & Mrs. Jones twins?
mom daughter son 1 son 2
8. The millionaire, Mr. Big, has just died. He has left behind a wife, daughter and
a large inheritance. The news of his death has brought forth 2 men who claim to
be the long lost son of Mr. & Mrs. Big. Before Mr. & Mrs. Big were married they
Multiple
Choice
had a child
taken from them. They had tried to find him but had no luck in
locating him. A DNA sample was taken from Mrs. Big, the Big daughter and the
two men who claim to be the long lost son. Which, if any, of the men are telling
the truth?
____
____
____
____
____
____
____
____
____
1. To produce genetically engineered bacteria that make a human protein, which of the following steps does
a scientist have to take first?
a. Insert the human gene for the protein into a plasmid.
b. Extract the protein from the bacterial culture.
c. Use a restriction enzyme to cut out the gene from human DNA.
d. Transform bacteria with the recombinant plasmid.
2. During genetic modification,
a. a prokaryote is changed into a eukaryote.
b. a cell takes in DNA from another source.
c. a cell’s own DNA is inserted into a plasmid.
d. a cell is mutated.
3. Analyzing DNA by gel electrophoresis allows researchers to
a. compare DNA samples from different sources.
b. determine whether a particular allele of a gene is dominant or recessive.
c. compare the phenotypes of different organisms.
d. cut DNA with restriction enzymes.
4. One function of gel electrophoresis is to
a. separate DNA fragments.
b. cut DNA.
c. recombine DNA.
d. extract DNA.
5. Knowing the sequence of an organism’s DNA allows researchers to
a. reproduce the organism.
b. mutate the DNA.
c. study specific genes.
d. cut the DNA.
6. Which of the following includes all the others?
a. plasmid
b. genetically modified bacterium
c. foreign gene
d. recombinant DNA
7. On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the
following statements is true?
a. Band B is more negatively charged than band A.
b. Band B moved faster than band A.
c. Band A is smaller than band B.
d. Band B consists of larger DNA fragments than does band A.
8. If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis,
the samples contained
a. the same amount of DNA.
b. fragments of the same size.
c. the same DNA molecules.
d. all of the above
9. What is an advantage of using genetically engineered bacteria to produce human proteins?
a. The human proteins produced by genetically engineered bacteria work better than those
produced by humans.
b. Genetically engineered bacteria can mass-produce pure human proteins.
c. The human proteins produced by genetically engineered bacteria last longer than those
produced by humans.
d. Genetically engineered bacteria can produce human proteins used to make plastics.