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GLOBAL WATER SAMPLING PROJECT FINAL REPORT ON SHARK RIVER SLOUGH NATIONAL EVERGLADES PARK BY FELIX VARELA SENIOR HIGH STUDENTS MIAMI, FL HONORS CHEMISTRY Period 5 MRS. CASAL Phosphates V. Balboa J. Bello C. Cano R. Font Background Information: Phosphates are chemical compounds containing phosphorous. Phosphorous is vital to human, animal, and plant life. Phosphates usually enter the water from “industrial waste”, “sewage discharge”, and agricultural runoff”. Phosphates are plant nutrients that stimulate the growth of algae. When too much algae is grown, this depletes the water body of oxygen Elemental Phosphorous was discovered accidentally in 1669 by a German chemist that was trying to make gold. Phosphates are used to make many products. For example, phosphates are found in cola drinks, they are used in detergents, and they are also used to preserve the moisture and taste in some foods such as ham and shrimp. A limiting factor for plant growth is a phosphate concentration of 0.02 parts per million (ppm). The Everglades are located in Southern Florida. It consists of extensive Marshlands. It has a limestone floor. In the Everglades, agricultural runoff destroys mats f composite algae called periphyton. This algae is essential, it provides oxygen and food to small aquatic organisms. It also helps many organisms to survive the dry season (December until April) until rain comes again. Shark River Slough is known as the “River of Grass”. Phosphate groups contain oxygen atoms and phosphorous. Phosphates act as a fertilizer for algae, they make it bloom. Algae is very important for fish because it is their food, but when the water contains excessive oxygen, it can be harmful. Thick layers of algae can block the sunlight from reaching the bed of the creek. This kills the plants that live at the deeper levels. Also, thick layers of algae act like a solar blanket absorbing heat from the sun causing the levels of the dissolved oxygen to decrease. Problem Statement: When testing the water collected from Shark River Slough (in the Everglades) will the phosphate results be high or low? Hypothesis: We hypothesize that the level of phosphates (measured in ppm) that the water obtained from Shark River Slough contained is normal, and relatively low. Procedures: First, rinse the test tubes so they are clean. Fill the test tube up to the 10 mL mark with the sample water (the water collected from Shark River Slough). Add 1.0mL Phosphate Acid Reagent using the pipette provided in the water test kit. Then, cap the test tube and invert it 3 times. Add 1 level measure of phosphate reducing reagent using the 0.1-gram spoon. Cap again and invert until you see that the reagent is dissolved. Wait 5 minutes. Remove the cap and place the test tube in the Nitrate-N and Phosphate Comparator with the Axial Reader. Match the color standards with the resulting color of the sample water tested, and record the results as ppm total phosphate. Data : Level of Phosphate in Shark River Slough Water Phosph ate level in 1 0.8 0.6 0.4 0.2 0 12 34 56 78 Series1 TRIALS (teams) Conclusions: As a group, we conducted tests on the water whish was collected from Shark River Slough, Everglades. The purpose was to test the Phosphate levels to see whether or not the water was at the right phosphate levels to consider it to be safe to drank by people and animals. Our findings were that the phosphate levels in all eight trials were the same: 1 ppm. This indicates that the water from Shark River Slough in the Everglades is at an excellent level to be induced. One part per million is a small amount which limits the growth of algae. As a group, we learned the importance of phosphates and their levels in water. Phosphates are what stimulate the growth of algae. Too much algae will led to the “depletion of the water body of oxygen”. However, algae feed certain animals that have certain bodies of water as their habitat. A way to keep the phosphate levels down could be to try to avoid dumping sewage and industrial waste into lakes, rivers, and oceans. This is our plan of action. Bibliography http://njnie.dl.stevens-tec.edu/curriculum/waterproj/phosphate.html www.go.grolier.com (Grolier Multimedia Encyclopedia) www.phosphates.com www. 101.com Nitrates G. Desir K. Cifuentes E. De la Cruz D. Sanchez Background Information: Nitrogen makes up a part of amino acids. The Earth’s atmosphere contains 78 percent of nitrogen. Through the nitrogen cycle, nitrogen gas is changed into nitrates to be used by living things. When plants and animals die, the compounds of nitrogen are decomposed into ammonia. Some plants make use of this ammonia and the rest is dispersed in water where microorganisms change it into nitrates and nitrites through the nitrification process. Nitrates may remain in decomposing humus or drained from the soil and transported to bodies of water. They may also be returned to the atmosphere by a process called denitrification. The Everglades is a far-ranging marshland covering parts of south Florida. It spreads over an area of 13,000 sq km (5,000 sq mi) and averages 80 km (50 mi) wide and 15 cm (6 in) deep The Everglades lie at the edge of the tropics. Its water flow provides for grasslands, thick forests, and various groups of animals, fish, and birds. The Everglades was formed between 8,000 and 10,000 years ago. After the latest Ice Age, the melting glaciers caused sea levels to rise. Overflowing from Lake Okeechobee the marshland was formed. It extends southwestward from to the shores of Florida Bay and the Gulf of Mexico. Small streams like the Miami, Little, and New rivers on the east and the Shark and Harney on the southwest, drain the region. Tidal bays, lakes, and waterways, with depths from 0.3 to 2.0 m (1 to 7 ft) weave through the diverse vegetation of the Everglades. The region lies less than 6 m (20 ft) above sea level, and includes elevation between 2 and 3 m (7 and 10 ft). The Everglades has a tropical savanna climate. The summers are hot and wet; the winters are warm and dry. The average annual rainfall is more than 130 mm (50) without including times when hurricanes pass the region or when the area may suffer from droughts. Vegetation in the Everglades includes cypress, mangrove brush, palms, oaks, pines and saw grass, which spread abundantly along the many islets. The formerly abundant wildlife in the area has decreased greatly in number and variety. However, alligators, American crocodiles, deer, birds, fish, snakes, and the endangered Florida panther are still in large quantity. Among the two sloughs of the Everglades National Park is the Shark River Slough, the "river of grass." The slough is the deeper and restless center of a broad marshy river. This "fast" flow moves at about 100 feet (30 meters) per day. Tree-islands grow on the slough and provide waters through channels from north to south. In the park waters, nutrients from agricultural overflow destroy algae called periphyton. Periphyton supply food and oxygen to the small organisms. In the dry season, this alga is very necessary because it provides the moisture that keeps many organisms alive until the rainy season. Also the natural balance is affected when salt water goes through aquifers when freshwater levels decrease. Due to the environmental issues the park faces, an eastern park will be added to promote wildlife. Problem Statement: What is the nitrate content in the water and how desirable is the condition? Hypothesis: Taking in consideration the weather/environmental conditions we believe that the nitrate content will be low and thus a fair to good condition. Data: TESTS Temperature (oC) PH Dissolved O2 (ppm) DO % Saturation Nitrates (ppm) Phosphates (ppm) BOD (ppm) Turbidity Coliform Bacteria Bromine Free Chlorine Alkalinity Trials (Teams) 1 2 26.7 26.7 oC oC 7.77 7.83 4ppm 4 ppm 9191110% 110% 0 0 AVG. 3 26.76 oC 7.85 4 ppm 91110% 0 4 26.76 oC 7.8 4 ppm 91110% 0 5 1ppm 1ppm 1ppm 8ppm 0JTU 8ppm 0JTU 1 1 180 0 0 180 7 26.0 oC 8 26.7 oC 7.9 8 ppm 91110% 0 6 26.7 oC 7.48 4 ppm 91110% 0 8 ppm 91110% 0 4 ppm 91110% 5 1ppm 1ppm 1ppm 1ppm 1ppm 1ppm 8ppm 0JTU 8ppm 0JTU 9ppm 0JTU 8ppm 0JTU 8ppm 0JTU 4ppm 0JTU 7ppm 0JTU 0 0 180 1 .5 180 1 0 180 1 1 180 1 0 180 0 0 180 0.0625 .31 180 26.7 oC 7.75 5 ppm 91110% .625 Bibliography: Microsoft Works 6.0 (1987-2001). Microsoft Encyclopedia Encarta 2002. Redmond, WA: Microsoft Corporation. “The Everglades-sloughs” available online at: http://www.nps.gov/ever/eco/habitats.htm “The Everglades-environment” available online at: http://www.nps.gov/ever/eco/h2omgmt.htm Turbidity J. Rodriguez R. Eckardt C. Rico K. Zepeda Background Information: When speaking about turbidity, one refers to how clear or cloudy the water is. When determining turbidity one looks at whether the water is transparent or opaque. If one possesses water, which is transparent, it has a low level of turbidity as opposed to water that is or seems to be opaque or cloudy. If the water is cloudy, the level of turbidity increases based on how clearly one can see through it. Causes of water turbidity include many things. Such things are natural in the water such as plankton soil and various sediments. Pollutants in the water such as raw sewage can also cause turbidity from neighboring cities. Items described above can enter the water system a number of ways. Sediments in the water can be circulated by activity in the water such as boating or by the inhabitants of the water (fish, whales, etc.). Plankton in water may occur if there are excessive nutrients, and if sewage happens to enter the water system, it is a result of cities releasing their waste into the water system. There will be many suspended particles in the water if the turbidity level is high. The particles, which are solid, block precious sunlight needed by life in the water such as the aquatic plants for performing photosynthesis. As a result of little or no photosynthesis the plants in the water produce less oxygen, thus reducing the dissolved oxygen levels of the water. The plants inhabiting the water will die effortlessly and be decayed by bacteria in the water. This will further decrease the dissolved oxygen levels of the water. The suspended particles in the water also soak up bonus heat from sunlight, which results in much warmer water. The fact is that warm water is not able to hold as much dissolved oxygen as cooler water, therefore the dissolved oxygen level will drop significantly in warmer water than colder water. These particles are also detrimental to the marine life of the water. These particles can obstruct the gills of fish from receiving fresh oxygen and interfere with the hunting abilities of the animals. Once these sediments settle, they can bury life living on the bottom of the body of water and these particles can carry pollutants throughout the water. Problem Statement: Is high turbidity of water detrimental to life in the water? What are respectable levels of turbidity in water? Hypothesis: It is hypothesized that the higher the level of turbidity in water, it is more detrimental to life that reside in that area of water. Respectable levels of turbidity are little or nothing, this is because the effects of turbidity vary from added heat to pollution eventually decreasing the dissolved oxygen levels of the water supply. Thus if there is little or no turbidity, the water is more welcoming to life because there will be nothing to inhibit life from prosperity. Procedures: There are many ways to determine the turbidity level of water. One such method is to use a Secchi disk. The Secchi disk is used by lowering it into the water until it’s no longer visible. Then elevated until it is seen a little and re-submerged until again it cannot be seen. The depth that the Secchi disk cannot be seen for the second time is documented in meters. This disk, known as the Secchi disk mainly used for waters that have a large depth. When measuring the level of turbidity for shallow waters, a turbidity test kit can be used. These kits include “an image at the bottom of a clear tube” (GWSCSEP), when used the visibility of the image determines the turbidity of the water. The level of turbidity is measured in units called the Jackson Turbidity Units, (JTU for short). When using a meter the data is recorded as Nephelometric Turbidity Units, (NTUs for short). In order to carry out the experiment you need to fill the test tube with sample water to indicated line. Place filled tube over the card on specified area. Looking through the tube, compare what is seen to the index card to determine turbidity of water sample. The results are recorded in JTU. Data: pH Dissolved Oxygen (ppm) DO% Saturation 7.77 7.83 7.85 7.8 7.9 7.8 7.48 4 4 4 4 8 4 8 91110% 91110% 91110% 91110% 91110% 91110% 91110% 7. 91110% Nitrates (ppm) 0 0 0 0 0 0 0 Phosphates (ppm) 1 1 1 1 1 1 1 BOD 8 8 8 8 4 8 8 Turbidity (JTU) 0 0 0 0 0 0 0 Coliform Bacteria Positive Positive Positive Positive Positive Positive Positive Bromine 1 0 0 1 1 1 1 Free Chlorine 1 0 0 0.5 0 1 0 180 180 180 180 180 180 180 Alkalinity Observations (color, smell, debris, etc.) 18 Water contained no visible debris. No strong foul odors were emitted. Hue of wat green. 120 100 100 100 100 100 100 100 100 100 80 60 40 20 0 0 1 Visibility Positive 0 2 0 3 0 4 0 5 0 6 0 7 0 8 Turbidity Conclusion: The result data showed that the quality of the water was overall good. The different test performed came out to 95% "excellent" condition. Considering the location of where the water samples were retrieved the water was in better condition then what was expected. PH, DO, and BOD were all at an excellent level. The data provided window to other water samples worldwide. In comparison with the water of Mill Hall Pennsylvania, Green River Wyoming, Peterborough Ontario Canada, and Sydney Australia, the water acquired from the Shark River sleuth has a very low level of turbidity. Most other cities that tested turbidity had around a 15 20 JTU average reading of water. The Shark River sleuth had very low levels. Recorded at a level below 5JTU. This information seems to suggest that the water in the Shark River Sleuth around the Everglades is in a healthy balance or for at least the moment. The global water sampling taught us as a group that there are many factors that can drastically affect homeostasis in an ecosystem. Water is an important part of the life cycle and all organisms in one way or another need it to survive. Facts that were learned from the results are that there might be several ways that we can improve the quality of the water, more over, to maintain the quality at optimum levels for to maintain a healthy ecosystem. Bibliography: Global Water Sampling Project, available online at: http://k12science.stevens-tech.edu/curriculum/waterproj/ References used in website: Water Words Dictionary USGS water resources USGS water resources for NJ Environmental protection agency Information about the Everglades National Park, available online at: http://www.gorp.com/gorp/resouce/us_Natonal_Park/fl_everg.HTM http://www.everglades.national-park.com/ http://www.nsp.gov/ever Dissolved Oxygen A. Ospina A. Hernandez T. Nutter D. Zambrano Background Information: Dissolved oxygen is the amount of gaseous oxygen dissolved in an aqueous solution. Oxygen dissolves into the water from the atmosphere until the water is completely saturated. Slowly the oxygen diffuses but the distribution depends on the rapid movement of the surrounding air. Fish, plants, bacteria, and invertebrates need this dissolved oxygen in order to breathe and to live. A higher dissolved oxygen level indicates better water quality. Water temperature can also affect dissolved oxygen levels. The higher the temperature, the less oxygen can de dissolved in the water. Most aquatic organisms need a dissolved oxygen level below 3 ppm. One fish will not survive in a dissolved oxygen level of 2 or 1 ppm. Growth and activity require a dissolved oxygen level of 5 to 6 ppm. Cold water can hold more dissolved oxygen than warm water. An important level of water quality is DO % saturation. Problem Statement: Is the dissolved oxygen level safe for certain organisms to live in? Hypothesis: We hypothesize that the Dissolved Oxygen levels will be in the accepted ranges for suitable living of organisms. Procedure: Fill test tube (completely) with sample water. Drop two-dissolved oxygen “Test-Tabs” into the test tube. Remove all air bubbles from test tube, and cap. Mix for about for about four minutes until tablets have dissolved Wait five minutes. Match the color of the sample (in test tube) to the dissolved oxygen color chart. Determine the dissolved oxygen PPM. Data: Dissolved Oxygen PPM 8 6 4 PPM 2 0 1 2 3 4 5 6 7 8 Trial Conclusions: We found that the dissolved oxygen ppm was at an average of 5ppm. It is in acceptable range, although ideally it should be around 8-10 ppm in order for organisms to thrive. Nonetheless 5 ppm is a good value. Anything less than 4 ppm, and the population of microorganisms in the water will be affected severely. There are, however, some microorganisms that can survive low DO conditions. The quality of the water is in fairly good state. As DO levels are concerned, it is healthy for organisms that live in it. Overall, we can safely say that the quality is good enough to support aquatic plant and animal life. Compared to the findings of other groups, based on the fall and spring 2001 data, the dissolved oxygen levels of our water sample were low. For instance, Central Mt. High in Pennsylvania recorded 16ppm for their fall 2001 data. Our water sample’s low 5 ppm look extremely low in comparison. However, it is within acceptable range. When compared to Georgetown School in Guyana, our 5 ppm isn’t that far away from their 6 ppm samples from pond water. From the water sampling project, we learned that the water is a habitat that needs to be carefully balanced. In our dissolved oxygen test, we learned that there must be a certain range in order for the water to support plant and animal aquatic life. We learned that our water source is in decent condition, but not in the condition that it should ideally be in. It is barely in acceptable conditions. Many factors can change the dissolved oxygen levels. We plan to inform the community of our findings through this project. Biological Oxygen Demand M. Masdeu, D. Persaud, K. Pichardo, K. Ho, P. Smith, R. Ajami, C.Llanos Background Information: Biological Oxygen Demand is also referred to as the Biochemical Oxygen Demand. Biological Oxygen Demand is the amount of oxygen required by microorganism’s to decompose the organic substances in a water sample. There are several steps involved in determining the BOD. During the conduction of the Global Water sampling project there were several steps involved. The first step is to obtain the amounts of water, and dilute each volume with distilled water. Then the water is shaken to ensure that the saturation of oxygen occurs. An oxygen meter is used to measure the concentration of oxygen within the container. The vials are then sealed and placed in a dark location with standard room temperature for five days or more. After the five days, the experimenters’ repeated the experiment to gather the BOD once again. The BOD was retained by subtracting the first reading for the result by the second result of the second experiment. Depending on the amount of water tested the results could be displayed in ppm, in which our scenario demonstrates. The more the Oxygen in demand is the greater the amount of organisms there are. The least amount signifies that an episode of extinction and a low organism rate. The Biological Oxygen Demand also relates to the dissolved Oxygen rate. The more organisms’, the greater the demand for oxygen is since it is the basis for life and a necessity for any organism. The dissolved oxygen rate is proportional due to the rate that oxygen is being consumed. The temperature also affects the BOD, because of the similarity in humans. When humans are in heat the their cardiovascular rate increases, and the BOD is increases. Like organisms when placed under harsh temperature the water temperature increases and the organisms consume more oxygen. The pH of the water can also be related to the BOD Shark River water sample. The pH displayed neutral and the water has a neutral base, which means that the water is nearly perfect. The BOD of the water sample is ideal for living organisms and proved to be the at most reliable through the series of tests. During this experiment, “Global Water Sampling Project,” a series of water tests were performed. This test in particular was conducted in Miami, Florida, and water sampling is a difficult decision although water is in abundance. The decision was made and the water sampling was performed from, “Shark River Slough,” a shark park extremely near the Everglades Park. Due to the Everglades being a National Park, samples could not be drawn. As a resort, a neighboring park, which shares the common natural water, served as the replacement for the water observations. The Everglades is the largest all natural endangered National Park in Florida, in which adequate water purity must be provided. The Global Water Sampling project will serve to compare water purity from around the world to provide a comparison in which a conclusion can be derived. Problem Statement: Will the BOD (Biological Oxygen Demand) levels of Shark River Slough prove the water to be polluted or unpolluted? Hypothesis: It is hypothesized that the BOD of the water samples taken from Shark River Slough will prove the water to be polluted with organic material. Because of the wide variety of organisms inhabiting Shark River Slough, the BOD will be at an elevated level. Because marine life produces waste, large amounts of bacteria would be needed to decompose all waste material. If there is a high amount of aerobic bacteria using dissolved oxygen (DO) to decompose the organic waste then the Biological Oxygen Demand will be unusually high. Procedures: In order to determine the BOD level of a sample of water, you must first collect two samples of water from a given water source. One of these samples must have its DO level taken immediately and recorded. The other sample must be wrapped in aluminum foil and stored in the dark for five days. After these five days the DO level of this sample must be taken and recorded. The difference between the DO of the two samples (ppm) is the BOD level of the water. Conclusions: The BOD levels of Snake River Slough were very high, proving that the water was polluted and also proving the hypothesis correct. The water sources average BOD was over 5ppm classifying it as “somewhat polluted” according to an article about BOD from the “Global Water Sampling Project’s” web page. This is most likely a result of the large amounts of bacteria that were found in the water that are needed to decompose the organic waste. The bacteria use a large amount of DO to perform this task. Strangely, the phosphate and nitrate levels of the water were low. Normally the nitrate/phosphate levels have a direct relationship with the BOD levels. The low nitrate and phosphate levels were inconsistent with the BOD levels. Future studies may include research and experimentation to conclude why this relationship was inverse. Because of the elevated BOD levels in the water, steps should be taken to help to improve the environment at Shark river Slough by avoiding the buildup of wastes in Shark River Slough and Everglades National Park. Bibliography : Global Water Sampling Project http://k12science.stevens-tech.edu/curriculum/waterproj/bod.html 1998-2001 Water Ecosystems and Water Pollution http://www.gpc.peachne.edu/~jaliff/water.htm Coliform Bacteria M. Magliano J. Mena M. Meneses Background Information: The test for coliform bacteria just like all other water tested done for this water project, is to test for any water impurities. Water that has run-off, pollution, sewage or any other waste, is usually infested with coliform bacteria. Coliform bacteria can be present in the water because of a virus or disease-producing bacteria that can be found in fecal material of animals or of man. Coliform bacteria are not a disease-causing organism. Usually when coliform bacteria are present in the water source, and visible, it is most often seen on the surface of water or at the bottom of the water with all the sediments. When there are coliform bacteria on the surface of the water this affects the dissolved oxygen and biological oxygen demand levels. The dissolved oxygen comes from the photosynthesis of plants. For plants to undergo photosynthesis they need the sunlight. If there are coliform bacteria on the surface of the water this blocks the sunlight from reaching the plants on the bottom of the water source. This means that there is less dissolved oxygen and a higher biological oxygen demand. The coliform bacteria test that took place in our experiment wasn’t that accurate. This test only lets us know whether or not there are coliform bacteria in the water or not. If the test results are positive we assume that this means that for every 100 mL of water there are 200 or more colonies of coliform bacteria present. To identify whether this water is good for drinking, swimming, boating/fishing further more accurate tests must be done. The results for these tests are as follows: 0 colonies/ per 100 mL of water, drinking water; 200 colonies/ per 100 mL of water, swimming water ; 1000 colonies/ per 100 mL of water, boating/fishing water. Some of the more accurate tests processes are the multiple tube fermentation MMO-Mug, the membrane filter, and the MPN. Some forms of disinfecting the water of coliform bacteria include the adding of chlorine, ozone iodine, ultraviolet light, steam sterilization, or boiling. Problem: What level of coliform bacteria is in the water sample collected at Shark River Valley Slough? Hypothesis: We hypothesize that the levels of coliform bacteria in the water sample from Shark River Valley Slough will be normal. Procedure: Fill the graduated cylinder with 10ml of the collected water being tested. Place the graduated cylinder on the indicated space of the sample card. Compare to the other diagrams and record. Data: Conclusions: The findings of the Coliform test were positive for coliform bacteria. The coliform bacteria test is an indicator of potability. This means that the water sample that we used in our experiment contained a high percentage of sewage water or decomposing waste. Relating to our water source, the water in the Everglades is contaminated by over 200 colonies per one hundred milliliters of water. All the findings for each test were positive for the bacteria. From these results, we can conclude that the water in the Everglades is terribly infested with coliform bacteria. Also, the water is nearly impossible for drinking and unsuitable for organisms. Our group also learned that the coliform bacteria were present in the water because of man and organisms. Man and organisms could have placed the bacteria in the water is because fecal coliform bacteria is found in humans and animals in the digestive system. The bacteria help to digest their food. Most importantly, this test can be so helpful with our society. The test can help people find out if their water is good to drink or if it can cause serious diseases. Due to the seriousness of the situation, we should take serious actions to help the Everglades. Many people do not know how contaminated the water is with waste. As a community, we all should watch what we take or have when we are near the Everglade water. Chemicals from food or drinks can mix with the water to form coliform bacteria. Therefore, there should be more strict regulations when visiting the Everglades. As a community, we should write letters to our representatives. We need to receive money to filter the Everglades water or it could be part of the restoration project already taking place. This crisis can not only happen in the Everglades but in other places as well. All civilians should learn to respect the rules and not liter. It will not only create a hazardous environment for organisms but us as well. Bibliography Njnie.dl.stevens-tech.edu/curriculum/waterproj/ www.state. Ky.us/nrepc/water/wcpfcol.htm wilkes.edu/~epc/coliform.htm pH Levels J. Motes S. Bardina N. Paz J. Rojas Background Information: pH measures the relative acidity of the water. Neutral pH level is 7.0. Pure water has a pH of 7.0. W ater with a pH level less than 7.0 is considered to be basic/alkaline. In the United States, the pH of natural water is usually between 6.5 and 8.5. Fresh water sources with a pH below 5 or above 9.5 may not be able to sustain plant or animal species. Industries and motor vehicles release nitrogen oxides and sulfur oxides into the environment. W hen these emissions combine with water vapor in the atmosphere, acids are formed! These acids accumulate in the clouds and fall to earth as acid rain or acid snow. Acid rain damages trees, crops, and buildings, and it can make lakes and rivers so acidic that aquatic organisms cannot survive. Problem Statement: What is the measure of the pH level on the Shark river Slough. Hypothesis: It is hypothesized that The Shark River Slough’s pH level will be mostly neutral with is a measure of 7. Procedures: In order to perform this test you should use a pH test kit or a pH paper. Simply follow the directions provided with the kit. pH must be measured immediately at the site because changes in temperature affect pH value. pH is recorded with a number value only, because there are no units associated with pH value. Try to take the water sample at a location away from the bank and below the river. Conclusions: In doing these tests, 8 trials were performed and there was an average of 7.79 for pH level in The Shark River Slough. This tells us that our water quality is not bad water due to the fact that water with a pH level greater than 7.0 is considered to be basic. Another reason why the water would not be considered bad is because in order to not be able to sustain plant or animal species the pH level would have to be below 5 or above 9.5. By comparing our data to the other group members we came to the conclusion that test performed by other groups doing similar projects have come to basically the same results, with pH level ranging from 7.4 to 7.9. In performing this experiment as a whole we learned that in order to truly analyze the quality of water it goes beyond the mere appearance. Actual tests are needed to determine what kind of water is found in different areas of our city and if this water is feasible for plant’s and animals as well as other organisms to live in. Bibliography: Center for Improved Engineering and Science Education/Stevens Institute of Technology- “pH water test”- Available at: http://k12science.stevens-tech.edu/curriculum/waterproj/ph.html Alkalinity, Bromine and Chlorine A. González J. Harris M. Latorre A. Montaño Background Information: Bromine is used to determine the amount of filtered and unfiltered mercury in water samples. The minimum-reporting limit for this reporting method is 0.04ng/l. The range of this method is from approximately 1 part per million to the part per million range. Many mercury compounds are highly toxic if swallowed or inhaled. There are many sources of mercury to the atmosphere, both natural and human related. Natural sources include oceans, volcanoes, and natural mercury deposits. Coal combustion, waste incineration, metal processing are the dominant human-related sources to the atmosphere. In ecosystems for which atmospheric deposition is the dominant source, resulting concentrations of total mercury in water are very low, generally less than 10 monograms per liter (ng/L). The challenge to scientists is to explain the series of processes that lead to toxic or near-toxic levels of mercury in organisms near the top of the food chain (bioaccumulation), when aqueous concentrations and source-delivery rates are so low. Chlorine is use to disinfect water and to kill bacteria or other harmful organisms (Cholera, typhoid, and dysentery). It makes water safe to drink. It has also saved a lot of lives. Sometimes, chlorine can have some effects on plants. Gardeners leave the water un-covered for 24 hours before watering. Chlorine is added to the water depending in the amount that is needed for the water to be treated. The dose is between 0.3 and 0.4 mg/l. However, Chlorine is added into water in amounts that cause no harm to humans. Chlorine gives a bad taste to the water. Higher levels of chlorine are mixed in water from lakes, rivers and reservoirs because they need more intense disinfestations. Chlorine may react with naturally organic substances and produce traces of trihalomethanes, THMs. Alkalinity facts: Alkalinity: waters capability to neutralize in acid. Alkalinity is a buffer A buffer is acids addition in a solution with out the changing of hydrogen ions or the pH level. Alkalinity is expressed in parts-per-million or milligrams- per liter of calcium carbonate Ponds and lakes with high alkalinity levels remain more resistant to acid rain or by any other means of contaminates in water. Alkalinity in combination of limestone creates wonderful safe Ph levels for plant or living life in water. When working with limestone alkalinity keeps a long-term hold in ponds and lakes. As with any excessive amount of a substance too much alkalinity in water creates corrosiveness and makes water undrinkable. We use alkalinity to stabilize pH levels, such as in ponds were suitable living conditions for plant and other life range between 6.0 to 9.0. In swimming pools, a moderate alkalinity level needs to range between 140 m/gl. Note if alkalinity levels are very high water samples become very corrosive and life contained in the high-alkalized water will die, also a slower process of neutralization takes place when alkaline levels reach higher than the recommended. Problem Statement: Will the alkaline, bromine and chlorine levels fall inside the normal range so the water can be safe for organisms? Hypothesis: If we test water for alkaline, bromine, and chlorine then we will find out that the levels are in the normal range for the organisms to live in a safe environment. Procedures: Gather all the materials Fill the beaker with water up to the line Dip the strip of paper provided into the water Wait about 10 seconds until the colors appear Take the strip of paper out of the water; do not remove excess water from the strip Observe and record data Bromine, Alkalinity and Chlorine Charts. 200 150 BROMINE FREE CHLORINE ALKALINITY 100 50 0 1 2 3 4 5 6 7 8 9 BROMINE FREE CHLORINE 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 ALKALINITY 1 2 3 4 5 6 7 8 9 Conclusions: We found that the alkalinity levels were higher than expected. The Bromine and Chlorine levels were low. This tells us that the quality of the water is pretty good. In the Everglades there is high alkalinity because of the limestone that there are there. The Chlorine and Bromine levels are normal because the water seems to not be contaminated. We were not able to check this data with the one of the other groups because there is no record of it in the Global water sampling project website. We learned that our Everglades water is in a healthy condition for organisms to live there. To keep our water safe and clean for the organisms that live there we should be allowed to take water samples from time to time to check the condition of the water. We also should keep our Everglades clean by not throwing garbage or any toxic waste there. Bibliography: Information on Everglades available online at: http://sofia.usgs.gov/publications/circular/1207/glossary.html Information about water tests available online at: www.plymouthwater.com/content/Chlorine.asp - 13k http://www.extension.umn.edu/info-u/environment/BD312.html http://www.wessexwater.co.uk/pdfs/chlorinefacts.pdf http://water.nr.state.ky.us/ww/ramp/rmalk.htm http://www.wvu.edu/~agexten/landrec/leachbed.htm http://www.utextension.utk.edu/aquafish/Generalaquaculture/GAbulletins/alkalinity. http://www.centerx.gseis.ucla.edu/globe/protocols/alklnty.htm aaawatertesting.com