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Immunology of Infection, Vol 37. Edition No. 3. Methods in Microbiology
Description:
Immunology of Infection, 3e, edited by two leading experts in the field, presents the most appropriate up-todate experimental approaches in the detail required for modern microbiological research. Focusing on the
methods most useful for the microbiologist interested in analyzing host-pathogen relationships, this volume
will be essential reading for all researchers working in microbiology, immunology, virology, mycology and
parasitology. This new edition of Immunology of Infection provides ready-to-use "recipes" and the latest
emerging techniques as well as novel approaches to the tried and tested, established methods included in
the successful first edition. Methods in Microbiology is the most prestigious series devoted to techniques
and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to
provide you with tried and tested, cutting-edge protocols to directly benefit your research.
Contents:
Introduction
Kaufmann/Kabelitz
The immune response to infectious agents
I. General Methods
Radbruch A
Phenotyping and isolation of functionally distinct leukocyte populations
Schütze S
Magnetic isolation of subcellular compartments
Berzofsky J
Prediction of MHC ligands and T-cell epitopes
McMichael A
Quantitative analysis of T-cell responses
Schurr E
Genetics of susceptibility and resistance to infection
Wiker H
Proteomic approaches to study immunity in infection
Sioud M
siRNA as a tool to study immune responses in infection
Schröder JM
Isolation and characterization of antimicrobial peptides
Karin M
Toll
and NOD-like receptors in anti-infective immunity
von Andrian UH
Visualization of immune responses in vivo
II. Murine Models
A. In vitro analysis
Czuprynski CJ
Isolation of lymphocytes from infected animals
Busch DH
Killer cell assays
Kiyono H
Quantification of murine T-cell cytokine responses
Gordon S
Isolation and functional charactzerization of macrophages
B. In vivo analysis
Ehlers S
Measuring immune responses in vivo
Belkaid Y
Control of infection by regulatory T-cells
Stockinger B
Th17 cells in inflammation
C. Specific Models
Orme IM
Murine models of tuberculosis
Muller I
The leishmaniasis model
Autenrieth IB
Murine models of infection with extracellular bacteria
Flynn JL
Local immune responses in the lung
Romani L
Murine models of fungal infections
Powrie F
Immunity in the gut
Reddehase MJ
Murine cytomegalovirus infection
Lingnau K/von Gabain A
Boosting of immune responses by adjuvants
III. Human System
Banchereau J
Subpopulations of human dendritic cells
Yssel H
Measuring human cytokine responses
Seitzer U
Measuring immune responses in situ
Garcia JV
Humanized mice for the analysis of human immune responses
IV. Appendix
Benoist C
The NIH knock-out mouse project
Beutler B
Forward genetic analysis to understand innate immunity
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