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BIOLOGY 263, FALL 2001
“MIDTERM” PRACTICAL EXAMINATION
Date: "! 1 November 2001 ö
Laboratory Practical Stations. Answer the questions at each station. (56%, 4% per station)
1. a.
b.
2. a.
8. a.
b.
9. a.
b.
b.
3. a.
10. a.
b.
b.
4. a.
11. a.
b.
b.
5. a.
12. a.
b.
b.
6. a.
13. a.
b.
b.
7. a.
14. Streak and label your plate as directed.
b.
Hypothesis Evaluation. Is there statistically significant support for each of the following
hypotheses? (6%, 2% each)
1. Hypothesis = Penicillium notatum grows faster than Rhizopus stolonifer.
Data = Mean colony diameter of P. notatum after 3 days = 3.2 cm, Mean colony diameter of R.
stolonifer after 3 days = 6.9 cm,
p value = 0.0007
Circle one: Hypothesis Supported
Hypothesis NOT Supported
2. Hypothesis = Bindweed is more common in empty lots than in lawns.
Data = Mean # of bindweed per m2 in lawn = 14.5, Mean # of bindweed per m2 in empty lots = 19.2,
p value = 0.074
Circle one: Hypothesis B Supported Hypothesis B NOT Supported
3. Hypothesis = There are more Gram negative bacteria species in soil than Gram positive bacteria
species.
Data = Mean # of Gram negative bacteria species per cm3 soil sample = 16.9, Mean # of Gram
positive bacteria species per cm3 soil sample = 16.1,
p value = 0.048
Circle one: Hypothesis B Supported
Hypothesis B NOT Supported
Definitions. Define each BIOLOGICAL term in an accurate, concise, and lucid manner. (10%,
2% each)
1. Electrophoresis:
2. Genetic Drift:
3. p-value:
4. Positive Control Group:
5. Science:
Short Answer. Address each question in as concise and lucid a manner as possible. (10%)
1. Explain why human races are considered to be a social reality but are NOT considered to be
a biological reality. (4%)
2. Create a taxonomic key to the following organisms. (6%)
Chlamydomonas
Candida
Holodiscus
Escherichia
Data Analysis. Address each question in as concise and lucid a manner as possible. (12%)
1. Use the following four gels on which are shown TPA-25 alleles (all individuals are from the
same population) to calculate the allele and genotype frequencies for the population. Then
determine if the population is at Hardy-Weinberg equilibrium. (6%)
wells
2. Use the following data matrix to construct a phylogenetic tree. Be certain to illustrate each
characteristic on the tree. (6%)
wings
sting
metamorphosis
"waist"
tail appendage
Bee
wings
sting
holometabolous
narrow waist
no tail append.
Wasp
wings
sting
holometabolous
narrow waist
no tail append.
Potato Beetle
wings
no sting
holometabolous
broad waist
no tail append.
House Fly
wings
no sting
holometabolous
narrow waist
no tail append.
Cockroach
wings
no sting
hemimetabolous
broad waist
no tail append.
Springtail Outgroup
no wings
no sting
hemimetabolous
broad waist
tail appendage
Experimental Design. Use the back of this page to design a rigorous experiment with
appropriate control(s) to test the hypothesis that in a flour culture containing the confused flour
beetle (Tribolium confusum) and the red four beetle (Tribolium castaneum), the confused flour
beetle will become more numerous than the red flour beetle. (6%)