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Name BIOLOGY 263, FALL 2001 “MIDTERM” PRACTICAL EXAMINATION Date: "! 1 November 2001 ö Laboratory Practical Stations. Answer the questions at each station. (56%, 4% per station) 1. a. b. 2. a. 8. a. b. 9. a. b. b. 3. a. 10. a. b. b. 4. a. 11. a. b. b. 5. a. 12. a. b. b. 6. a. 13. a. b. b. 7. a. 14. Streak and label your plate as directed. b. Hypothesis Evaluation. Is there statistically significant support for each of the following hypotheses? (6%, 2% each) 1. Hypothesis = Penicillium notatum grows faster than Rhizopus stolonifer. Data = Mean colony diameter of P. notatum after 3 days = 3.2 cm, Mean colony diameter of R. stolonifer after 3 days = 6.9 cm, p value = 0.0007 Circle one: Hypothesis Supported Hypothesis NOT Supported 2. Hypothesis = Bindweed is more common in empty lots than in lawns. Data = Mean # of bindweed per m2 in lawn = 14.5, Mean # of bindweed per m2 in empty lots = 19.2, p value = 0.074 Circle one: Hypothesis B Supported Hypothesis B NOT Supported 3. Hypothesis = There are more Gram negative bacteria species in soil than Gram positive bacteria species. Data = Mean # of Gram negative bacteria species per cm3 soil sample = 16.9, Mean # of Gram positive bacteria species per cm3 soil sample = 16.1, p value = 0.048 Circle one: Hypothesis B Supported Hypothesis B NOT Supported Definitions. Define each BIOLOGICAL term in an accurate, concise, and lucid manner. (10%, 2% each) 1. Electrophoresis: 2. Genetic Drift: 3. p-value: 4. Positive Control Group: 5. Science: Short Answer. Address each question in as concise and lucid a manner as possible. (10%) 1. Explain why human races are considered to be a social reality but are NOT considered to be a biological reality. (4%) 2. Create a taxonomic key to the following organisms. (6%) Chlamydomonas Candida Holodiscus Escherichia Data Analysis. Address each question in as concise and lucid a manner as possible. (12%) 1. Use the following four gels on which are shown TPA-25 alleles (all individuals are from the same population) to calculate the allele and genotype frequencies for the population. Then determine if the population is at Hardy-Weinberg equilibrium. (6%) wells 2. Use the following data matrix to construct a phylogenetic tree. Be certain to illustrate each characteristic on the tree. (6%) wings sting metamorphosis "waist" tail appendage Bee wings sting holometabolous narrow waist no tail append. Wasp wings sting holometabolous narrow waist no tail append. Potato Beetle wings no sting holometabolous broad waist no tail append. House Fly wings no sting holometabolous narrow waist no tail append. Cockroach wings no sting hemimetabolous broad waist no tail append. Springtail Outgroup no wings no sting hemimetabolous broad waist tail appendage Experimental Design. Use the back of this page to design a rigorous experiment with appropriate control(s) to test the hypothesis that in a flour culture containing the confused flour beetle (Tribolium confusum) and the red four beetle (Tribolium castaneum), the confused flour beetle will become more numerous than the red flour beetle. (6%)