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BENEFICIAL MICROBES FOR THE SUSTAINABLE MANAGEMENT OF
SHRIMP AQUACULTURE
Dr. Karthik Ramachandran, Ph.D
Product Manager –Aquaculture
Guybro Chemicals Pvt Ltd, Corporate Office,
201, Maruti Chamber, Fun Republic Lane, Veera Desai Road, Andheri (West),
Mumbai – 400 053, Maharashtra, India
Email : [email protected] / [email protected]
Website: www.guybro.com Tel : 91-22-40546800 Fax : 91-22-40546811
Mobile : +91 90477 36561 / 90047 33761
Abstract
Aquaculture production has grown enormously in recent years and among that
Penaeid shrimps are one of the most important cultured species worldwide especially in Asia due
to their high economic value and export. Shrimps are infected by numerous pathogenic microbes
which caused high mortality. The excess feed and fecal matter in grow out ponds accumulating
in the impoundments may result in bacterial decomposition of organic matter in the sediment and
produce excess of toxic compounds like ammonia and also pollute the environment. Very limited
research has been carried out on the culture, growth performance and disease management in
aquaculture using beneficial microbes. Moreover, in the beginning years of aquaculture the
antibiotics were employed to kill the microbial pathogens and to enhance the growth of the
shrimps in intensive and semi-intensive types of culture. The excess use of antibiotics has
created the problem of residue buildup in tissue of shrimps, resistance of culture species to the
consistent use of antibiotics and rejection of export consignments etc. As a remediation of these
unpleasant and unwanted problems in aquaculture there is a need of beneficial microbes in
shrimp culture through feed and/or water to prevent the aquaculture ponds from undergoing
eutrophication and to control the microbial diseases in shrimps and to enhance their production
in an ecofriendly ambience without the use antibiotics thus, resulting in quality assurance,
quality control and above all environmental safety.
Keywords: Shrimp farming; Eutrophication; Microbial Diseases; Beneficial Microbes
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Introduction
Aquaculture is an emerging branch of biosciences boosting food production. The
marine fisheries stocks have declined worldwide in general and provided an impetus for rapid
development in fish and shellfish farming. In general, shrimp ponds are enclosed cultivation
systems, subject to periodic water renewal to compensate for volume changes (due to
evaporation) and salinity changes (evaporation, precipitation) and to maintain water quality. The
production process in shrimp aquaculture is determined by biological, technological, economical
and environmental factors (Yuvaraj and Karthik, 2015 b). Especially, the microbial diseases are
commonly implicated in episodes of mortality, so that our Indian farmers prefer to use
commercial antibiotics available in markets. However, the use of antibiotics has recently become
a major public concern because their use can lead to the development of drug resistant bacteria,
thereby reducing drug efficacy. Moreover, the accumulation of antibiotics both in the
environment and in shrimp tissues can be potentially risky to consumers and the environment.
Another most important problem faced by our Indian shrimp culture farmer is excess of
Ammonia in pond water and sediment which is caused by excess feed, fecal matter and dead
algae deposited in the bottom of the pond. Due to this, shrimps are exposed to toxic gases like
NH3, NO2 and H2S further leads to eutrophication in culture system and cause stress to the
animal and ultimately end with microbial diseases and high mortality occurs (Yuvaraj et al.,
2015 a).
Though shrimp culture has undergone rapid development in India, successful
production is increasingly hampered especially by diseases in addition to environmental
pollution and poor management practices. Of all the infectious diseases, bacterial infections
cause serious diseases like vibriosis, White spot, ulcer, tail rot, necrosis etc. Currently, antibiotics
have been widely used in shrimp aquaculture to control bacterial infections. However, the use of
antibiotics has recently become a major public concern because their use can lead to the
development of drug resistant bacteria, thereby reducing drug efficacy. Moreover, the
accumulation of antibiotics both in the environment and in shrimp tissues can be potentially risky
to consumers and the environment. Such adverse effects have prompted scientists to search for
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alternatives to replace antibiotics in controlling diseases in shrimp farms (Karthik et al., 2013 &
2014).
Role of beneficial microorganisms (BMs) in aquatic ecosystem
Nowadays, beneficial microorganisms (BMs) are used in aquaculture mainly for two
aspects: one is to regulate microdysbiosis, to maintain microeubiosis, to raise the health level of
hosts, and to promote the proliferation of BMs and their metabolic products in microecology.
The other is to decompose organic matter (feces, wastes, remnants or leftovers of feed), to
maintain dynamic ecological balance among organisms from the five kingdoms in nature and
microbial ecological equilibrium in water and sediments, and to create a friendly environment for
fish and shrimp growth (Zhou et al., 2009). Microbes include microalgae, bacteria, fungi and
virus. Microalgae mainly photosynthesize to absorb CO2 and to supply oxygen to aquatic
animals. The main role of bacteria and fungi is to decompose organic matter in sediments so as
to keep the water clean. However, due to the lack of oxygen there might be anaerobic
decomposition or fermentation in sediments, producing harmful gases such as hydrogen sulphide
and methane. There are two food chains in aquatic ecosystem: detritus food chain and prey–
predatory food chain.
Microbes are the predominant photosynthetic organisms in most aquatic environments.
In aerobic conditions (e.g. shallow water), algae, diatoms and Cyanobacteria predominate. In
anaerobic conditions (polluted or eutrophic waters), other photosynthetic bacteria are dominant.
There is a big difference between natural aquatic ecosystem and man-made aquatic ecosystem,
especially in intensive culture with high-stocking density and high input. The water body in a
high-stocking density fishpond often in a small area has a limited self-purification capability.
Therefore, there is a need to add BMs in stages to improve water quality to reduce the prevalence
of fish diseases. When BM population in water body grows to a certain extent forming a specific
population, BMs would hinder the growth of harmful ones during the competition for survival,
thus the pond water is in a balanced and stable microbial ecological condition conducive to the
growth of aquatic animals (Karthik et al., 2015 a-d).
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Beneficial role of bacteria
In normal, diseases in aquaculture practices are mostly caused by luminous bacteria
Vibrio harveyi, and it has been referred as the largest economic loss in the shrimp aquaculture
due to mass mortalities (Sivakumar et al., 2014). To control the pathogens, the use of beneficial
microbes in aquaculture is increasing demand for its more environment friendly aquaculture
practices (Petlu Nitya et al., 2013). Nowadays, the use of beneficial microbes in aquaculture
might represent a valuable mechanism to increase shrimp growth and survival rate. In general,
the gastro intestinal tract (GIT) of the aquatic animal is mainly composed of gram negative
bacteria (Vine et al., 2006). Researchers also have demonstrated about the use of beneficial
bacteria in aquaculture to improve the water quality and immune system by balancing bacterial
flora in water and reducing pathogenic bacterial load (Kesarcodi-Watson et al., 2008).
Recently the researchers have been attempting to isolate beneficial bacteria from
various sources like soil, water and animal gut to control disease causing pathogens in
aquaculture systems (Austin and Day 1990; Munro et al., 1995; Gomez-Gil et al., 2000; Ahmed
et al., 2005; Skrodenyte- Arbaciauskiene et al., 2006; Kim et al., 2007 and Bestha Lakshmi et
al., 2013). Beneficial microbes that currently used in aquaculture industry include a wide range
of taxa – from Lactobacillus, Bifidobacterium, Pediococcus, Lake Streptococcus sp and
Carnobacterium sp. Bacillus, Flavobacterium, Cytophaga, Pseudomonas, Alteromonas,
Aeromonas,
Enterococcus,
Nitrosomonas,
Nitrobacter
and
Vibrio
spp.,
and
yeast
Saccharomyces, Debaryomyc (Irianto and Austin 2002; Sahu et al., 2008 and Hemaiswarya et
al., 2013). While using some beneficial bacteria for fish, some might be highly pathogenic Vibrio
alginolyticus and lead to destructive effect in the aquaculture systems. Therefore, it is necessary
to take care of the choice of beneficial microbes before administration. The best known
beneficial bacteria such as Bifidobacteria species, Lactobacillus sp and Streptococcus
thermophilus are employed as the dietary supplementation with feed in aquaculture industry and
increased the efficiency and sustainability of aquaculture production (Kim et al., 2007).
Beneficial microbes thus fulfill the concept of sustainable growth and production of shrimps by
minimizing or de-promoting the growth of the disease causing harmful pathogens.
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Beneficial role of microalgae
Diatoms are always dominant in ponds of good quality. As organic pollution increases,
Pyrrophyta sp, Cyanophyta sp and Cryptophyta sp dominate instead of diatoms. Literature has
revealed that, diatoms secrete anti-bacterial substances and good food for post larvae of Penaeus
shrimp. Some diatoms such as Chaetoceros sp can rapidly reduce ammonia nitrogen. In order to
maintain the good quality of pond water, some scholars have suggested that inoculation of
diatoms to predominate the probiotic system would be a prudent approach. However, it is not
easy to inoculate diatoms for they need severe growth conditions. The kind of ecological
environment that is required to promote the growth of diatoms has not been reported so far (Xu,
2006). Austin et al., (1992) reported a kind of microalgae (Tetraselmis suecica), which can
inhibit pathogenic bacteria of fish. Teraselmis suecica was observed to inhibit Aeromonos
hydrophila, A. salmonicida, Serrstia liquefaciens, Vibrio anguillaram, V. salmonicida and
Yersnia ruckeri type I. When used as a food supplement, the algal cells inhibited laboratory
induced infection in Atlantic salmon. When used therapeutically, the algal cells and their extracts
reduced mortalities caused by A. salmonicida, Serrstia liquefaciens, V. anguillaram,
V. salmonicida and Yersnia ruckeri type I. They suggested that there may be some bioactive
compounds in the algal cells, and there appears to be a significant role for Tetraselmis in the
control of fish diseases (Ramalingam et al., 2015 a&b).
Bioremediation in aquaculture pond
Biodeterioration in water bodies can be alleviated by bioremediation, using individual
or combined organisms to minimize the effluents from aquaculture or any other industries.
Major advantages of bioremediation are:
1)
It can be done on site and the process does not lead to any site disruption.
2)
There is every possibility for permanent waste elimination.
3)
Being a biological process it will be comparatively too inexpensive.
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Bioremediation of aquaculture pond waste using microbes
Water quality in aquaculture systems are controlled through microorganisms by
microbial degradation of organic matter (Anderson et al., 1987; Rheinheimer, 1992 and
Avnimelech et al., 1995). The dissolved and suspended organic matter contains mainly carbon
chains and is highly available to microbes and algae and it helps the microbes to multiply rapidly
and have good enzymatic capability. The bacteria such as, Bacillus subtilis, B.licheniformis,
B.cereus, B.coagulans were used as suitable for bioremediation of organic detritus (Sharma,
1999 and Singh et al., 2001). Lactobacillus sp are also used along with Bacillus sp to break down
the organic detritus. These bacteria produce a variety of enzymes that break down proteins and
starch to small molecules, which are then taken up as energy sources by other organisms. The
removal of large organic compounds reduces water turbidity (Haung, 2003). Many examples in
which both singular bacterial strains and microbial systems have been successfully utilized to
reduce and or transform selected pollutants to nontoxic molecule in laboratory conditions
(Eschenhagen et al., 2003; Gallizia et al., 2003; Gallizia et al., 2005 and Bhaarathi Rajan et al.,
2015).
MECHANISM OF BENEFICIAL MICROBES
(ENDOCOMMENSALS/ NONSPECIFIC)
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SYSTEM BIOLOGICAL APPROACH TO AQUACULTURE
Conclusion:
Shrimp aquaculture is burdened with environmental problems that arise from the
consumption of resources, such as land, water, seed, feed, their transformation into products
valued by society. In addition after the culture period, effluents from aquaculture ponds contain
living and dead particulate organic matter, dissolved organic matter, ammonia, nitrite, nitrate,
phosphate, suspended soil particles, and other substances that can be considered potential
pollutants. Hence, application of beneficial microbes in aquaculture systems has been rising over
the years with increasing demand for more environment friendly aquaculture practices. The use
of beneficial microbes as beneficial microbes in the previous research studies revealed
significant reduction in the CFU of the pathogenic bacterial species. This findings is of interest
since the pathogenic forms of bacterial species will have quarom sensing mechanisms which is
mainly distributed to their cytopathogenicity in the host forms. By containing their population
buildup the beneficial microbes also contain or repners their quorum sensing genes operation.
Thus the efficacy of beneficial microbes is proved by several researchers. Hence, it could be
concluded that the extrapolation of beneficial microbes as feed and/or water beneficial microbes
in shrimp culture will definitely prevent the aquaculture ponds from undergoing organic matter
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accumulation, ammonification, eutrophication and prevent the environment from pollution and
also control the microbial diseases to the shrimps and enhance the productivity of the farms to
the benefit of local economies in an ecofriendly ambience without antibiotics.
References
1.
Ahmed H, Al Harbi T and Uddin M N,2005. Bacterial diversity of tilapia (Oreochromis
niloticus) cultured in brackishwater in Saudi Arabia. Aquaculture, 250:566-572.
2.
Anderson R K, Parker P L and Lawrence A, 1987. A C3/C12 tracer study of the
utilization of presented feed by a commercially important shrimp Penaeus vannamei in a
pond grow out system. Journal of the World Aquaculture Society, 18, 148-155.
3.
Austin B, E Baudet, and M Stobie, 1992. Inhibition of bacterial fish pathogens by
Tetraselmis suecica. Journal of Fish Diseases, 90, 389–392.
4.
Austin, B and Day, J G, 1990. Inhibition of prawn pathogenic Vibrio spp by a
commercial spraydried preparation of Tetraselmis suecica. Aquaculture 90, 389-392.
5.
Avnimelech Y, Mozes N and Kochba M. 1995. Rates of organic carbon and nitrogen
degradation in intensive fish ponds. Aquaculture, 134, 211 - 216.
6.
Bhaarathi Rajan UD, R. Karthik, K. Ramalingam and R. Muthezhilan, 2015.
Antibacterial effectiveness of hemolymph serum of Scylla tranquebarica inoculated by
Pseudomonas aeruginosa ATCC 27853, International Journal of Fisheries and Aquatic
Studies, 2(6): 43-45.
7.
Bestha Lakshmi, Buddolla Viswanath and Sai Gopal DVR, 2013. Probiotics as Antiviral
Agents in Shrimp Aquaculture. Journal of Pathogens, 10:23.
8.
Eschenhagen, M, Schuppler and M Roske, I. 2003. Molecular characterization of the
microbial community structure in two activated sludge systems for the advanced
treatment of domestic effluents. Water Research, 37, 3224–232.
9.
Gallizia, I. McClean, S. Banat, I.M. 2003. Bacterial degradation of phenol and 2, 4Dichlorophenicol . Journal of Chemical Technology and Biotechnology 78, 959–963.
10.
Gallizia, I. Vezzuli, L. Fabiano, M, 2005. Evaluation of different bioremediation
protocols to enhance decomposition of organic polymers in harbor sediments.
Biodegradation, 16, 569-579.
8
11.
Gomez Gil B, Roque A and Turnbull J F, 2000. The use and selection of probiotic
bacteria for use in the culture of larval aquatic organisms. Aquaculture, 191: 259-270.
12.
Haung H J, 2003. Important tools to the success of shrimp aquaculture- Aeration and the
applications of tea seed cake and probiotics. Aqua International, 13-16.
13.
Hemaiswarya S, Raja R, Ravikumar R and Carvalho IS, 2013. Mechanism of Action of
Probiotics. Braz Arch Biol Technol, 56:113-119.
14.
Irianto A and Austin B, 2002. Use of probiotics to control furunculosis in rainbow trout,
Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 25,333-342.
15.
Karthik R, Angelin C Pushpam, K. Ramalingam, D. Yuvaraj and M. C. Vanitha, 2015
(a). Attenuation of negative impacts by micro algae and enriched Artemia salina on
Penaeus monodon and Litopenaeus vannamei larval culture, Journal of Fisheries and
Aquatic Science, 10 (5): 347-356.
16.
Karthik R, Angelin C Pushpam, Yashika Chelvan and M. C. Vanitha, 2015 (b). Efficacy
of probiotic and nitrifier bacterial consortium for the enhancement of Litopenaeus
vannamei aquaculture, International Journal of Veterinary Science and Research, 2(1):
001-006.
17.
Karthik R, Angelin C Pushpam, and M. C. Vanitha and D. Yuvaraj, 2015 (c).
Development of Marine Derived Probiotic Bacterial Consortium For The Sustainable
Management of Litopenaeus Vannamei Culture. International Journal of Advanced
Research in Engineering and Technology, 6(10), 62-75.
18.
Karthik R, Angelin C Pushpam, Yashika Chelvan and Vanitha MC, 2015 (d). Efficacy of
Cheatoceros Calcitrans, Enriched Artemia Salina, Bacillus stratosphericus (AMET1601)
nov., and Nitrifier Bacterial Consortium as Probiotics on Litopenaeus Vannamei, Journal
of Fisheriessciences.com (Impact Factor: 1.5), 9 (4); 41-48.9(
19.
Karthik R, A. Jaffar Hussain and R. Muthezhilan, 2014. Effectiveness of Lactobacillus sp
(AMET1506) as probiotic against Vibriosis in Penaeus monodon and Litopenaeus
vannamei shrimp aquaculture. Biosciences Biotechnology Research Asia, 11: 297-305.
20.
Karthik R, S. Gobalakrishnan, A. Jaffar Hussain and R.Muthezhilan 2013. Efficacy of
Bacteriocin from Lactobacillus Sp. (AMET 1506) as a biopreservative for seafood’s
under different storage temperature conditions. Journal of Modern Biotechnology, 2 (3):
59–65.
9
21.
Kesarcodi Watson A, Kaspar H, Lategan M J and Gibson L, 2008. Probiotics in
aquaculture, The need, principles and mechanisms of action and screening processes,
Aquaculture, 274:1-14.
22.
Kim DH, Brunt J, Austin B, 2007. Microbial diversity of intestinal contents and mucus in
rainbow trout (Oncorhynchus mykiss). J Applied Microbiol, 102:1654-1664.
23.
Munro P D, Barbour A, Birkbeck TH,1995. Comparison of the growth and survival of
larval turbot in the absence of culturable bacteria with those in the presence of Vibrio
anguillarum, Vibrio alginolyticus, or a marine Aeromonas sp. Appl Environ Microbiol,
61:4425–4428.
24.
Petlu Nitya Jeevan Kumar, Sangeetham Jyothsna, Malapati Hanuma Reddy and
Sreemanthula Sreevani, 2013. Effect of Bacillus subtilis and Lactobacillus rhamnosus
incorporated probiotic diet on growth pattern and enzymes in Penaeus vannamei, 3: 2
250-480.
25.
Ramalingam K, S. Ramarani, R. Karthik and R. Muthezhilan, 2015 (a). Status of
electrolytes and trace elements in the tissue of Macrobrachium rosenbergii (De Man)
inoculated by Pseudomonas aeruginosa MTCC 1688. International Journal of pure and
Applied Bioscience, 3 (2): 456-461.
26.
Ramalingam K, D.R. Shyamala and N. Sri Kumaran, R. Karthik and M. C. Vanitha, 2015
(b). Manifestations studies on enzyme profile of Vibrio parahaemolyticus MTCC451
inoculated black tiger prawn Penaeus monodon, Journal of Fisheries and Aquatic
Science, 10 (6): 477-488.
27.
Rheinheimer G, 1992. Pathogens in aquatics plants and animals and their control. In:
Rheinheimer G (ed) Aquatic microbiology, 4th edn. John Wiley and Sons, Guildford,
175–249.
28.
Sahu MK, Swarnakumar NS, Sivakumar K, Thangaradjou T, Kannan L, 2008. Probiotics
in aquaculture: importance and future perspectives. Indian J Microbio, 48:299-308.
29.
Sharma R, Scheeno TP, 1999. Aquaculture wastes and its management. Fisheries World,
22-24.
30.
Singh ISB, Radhika MH, 2001. Photosynthetic sulphur bacterium in the bioremediation
of hydrogen sulphide toxicity in grow-out systems.19:47-49.
10
31.
Sivakumar, N. G. Selvakumar, P. Varalakshmi and B. Ashokkumar, 2014. Lactobacillus
sp. A potent probiotic for disease free shrimp aquaculture. Int. J. Recent Scient. Res. 5:
1031-1045.
32.
Skrodenyte-Arbaciauskiene V, Sruoga A, Butkauskas D, 2006. Assessment of microbial
diversity in the river trout Salmo trutta fario L. intestinal tract identified by partial 16S
rRNA gene sequence analysis. Fisheries Sci, 72:597- 602.
33.
Vine N.G, Leukes W. D, Kaisher H, 2006. Probiotics in marine larviculture, FEMS
Microbiol, 30, 404-427.
34.
Xu, Y. Wahlund, T. M. Feng, L. Shaked, Y. and Morel, F. M. M, 2006. A novel alkaline
phosphatase in the coccolithophore Emiliania huxleyi (Prymnesiophyceae) and its
regulation by phosphorus, J. Phycol, 42, 835–844.
35.
Yuvaraj D, R. Karthik and R.Muthezhilan, 2015 (a). Crop rotation as a better sanitary
practice for the sustainable management of Litopenaeus vannamei Culture, Asian Journal
of Crop Science, 7 (3): 219-232.
36.
Yuvaraj D and R. Karthik, 2015 (b). Efficacy of Probiotics on Litopenaeus vannamei
Culture through Zero Water Exchange System, Journal of Fisheries and Aquatic Science,
10 (6): 445-463.
37.
Zhou X, Wang Y and Li W, 2009. Effect of probiotic on larvae shrimp (Penaeus
vannamei) based on water quality, survival rate and digestive enzyme activities.
Aquaculture, 287: 349-353.
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