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Unit 1 The Power of Reproduction Chapter 1 – The Cell Cycle and Asexual Reproduction Animal Cell Plant Cell 1.1 The Cell (Pages 4 – 16) 1. 2. Scientists have long been fascinated with life. After thousands of years of studying it we still haven’t figured out how it works. Some history: Assignment: a) b) c) The Cell Question Sheet Label Cell Diagrams (1-6 & 1-7) Cell Structures Chart Chapter 1.1 – The Cell Question Sheet 1. Record the date and contribution to cell theory for the following scientists: (22) a) b) c) Aristotle (384-322 BC) – animal and plant kingdom (animals higher, spontaneous generation) Zacharias Janssen (1590) – first compound microscope Robert Hooke (1665) – microscope – referred to cells as “rooms” Chapter 1.1 – The Cell Question Sheet 1. Record the date and contribution to cell theory for the following scientists: (22) d) e) John Ray (1667) – “species” organism that reproduce with their own kind Francesco Redi (1668) – disproved spontaneous generation (flies and maggots) The Redi experiment Replica of a Leeuwenhoek microscope The following photomicrographs were taken through a Leeuwenhoek microscope. f) g) h) i) Anton van Leeuwenhoek (1632 – 1723) microscope Robert Brown - nucleus is part of the cell Schleiden/Schwann (1838/1839) – all animals are made of cells Alexander Carl Heinrich Braun (1845) – the cell is the basic unit of life • • • Charles Darwin and Alfred Wallace – species form variations Rudolph Virchow (1821 – 1902) – cells come from cells Louis Pasteur (1860) – disproved spontaneous generation at the microscopic level (life comes from life) Pasteur’s swan neck flask experiment 2. Like all sciences, biology has its own set of tools, techniques, and investigative methods. What tools helped scientists develop the cell theory? (3) 3. What methods helped scientists develop the cell theory? (2) 4. The internet was originally designed to allow scientists all over the world to communicate quickly and easily with each other. What invention had a similar effect on scientific communication in earlier centuries? (1) 5. What can you conclude about the relationships between scientific discovery, tool inventions, and new methods? (1) Cell theory states that: (4) 6. • • • • all living organisms are made up of one or more cells Cells are the basic unit of life Cells come from cells Activity of entire organism depends on total activity of its independent cells Animal Cell Plant Cell Assignment BLM 1-5, 1-8, 1-9, 1-10, Investigation 1B Cell Structures Chart Cell Size and Scale • Nucleus • Nuclear Membrane • DNA Stands for: 4. Chromatin 5. Nucleolus 6. Ribosomes 7. Cell Membrane 8. Cytoplasm 9. Endoplasmic Reticulum 10. Mitochondria 11. Golgi Bodies 12. Vacuoles 13. Lysosomes 13. Cell Wall (Plants only) 13. Chloroplasts (Plants only) 1.2 Understanding the Cell Cycle Cells grow and then divide to make new cells. New cells are used to replace dead ones OR for growth of the organism. YOU started out as 1 cell! Before a cell can divide into 2 cells it must produce almost twice as many organelles (replication). A cell’s stages of life are called phases. 1. Interphase • Most of cell’s life • DNA in thin strands called Chromatin replicate. • Chromatin coils up to form double stranded Chromosomes. • A Centromere connects the original chromatin with its identical replicate. • The cell has a complete extra copy of DNA. A Chromosome 2. Prophase • Duplicate DNA is easily seen under microscope. • Nucleolus and Nuclear Membrane disappear. • Centrioles move to opposite sides of the cell. • Spindle fibres (like a scaffold) grow out of each centriole and attach to centromere. 3. Metaphase • Spindle fibres pull on centromeres • Chromosomes move to line up in the middle. 4. Anaphase • Spindle fibres shorten and pull centromere apart. • One copy of DNA goes to each side. 5. • • • • Telophase A complete set of chromosomes arrives at each centriole. Spindle fibres disappear. Nuclei and nucleoli form. Chromosomes uncoil into thin chromatin. 5. • • • Telophase Cell membrane pinches together in the middle Two cells form (animal cells). A cell plate grows across the middle of the cell forming a new cell wall between the two cells. (plants) Parent Cell Daughter Cells Phase 1: Prophase Phase 2: Metaphas e Phase 3: Anaphase Phase 4: Telophase Mitosis Animation Clip http://www.pbs.org/wgbh/nova/miracle/divide.html# http://www.johnkyrk.com/mitosis.html http://www.dnatube.com/video/1148/Mitosis http://www.dnatube.com/video/2380/InterpretiveMitosis Assignment BLM’s 1-14, 1-15, 1-16 & 1-17 Mitosis Crossword & Mitosis Chart Mitosis Quiz 1.3 The Cell Cycle in YOUR Body Some common chromosome counts: Dogs Tomatoes Humans Black Molly (fish) 78 24 46 46 1. Normal Cell Replacement a) Cells die of old age and need to be replaced. See pg 25 for cell life spans. b) c) about 3 000 000 cells die in your body every minute. Cells die due to damage or when they don’t get enough food or oxygen. Regeneration - Healing of damaged tissue or the replacement of body parts is called regeneration. (NOTE: see note under Figure 1.17 – pg 26) 2. Growth a) b) As organisms grow larger, their cells stay the same size They just get more of them. Aging 1. 2. Aging is connected to the slowing of the cell cycle. The cells do not divide as often or as quickly. Cancer is caused when the DNA of a cell becomes damaged by 1. a) b) c) d) e) f) Tobacco Asbestos Certain chemicals some viruses Radioactivity UV radiation 2. 3. 4. 5. 6. The damage injures but does not kill the cell. The cell no longer functions properly and the DNA no longer has correct information about when and how quickly to divide. The result: Useless cells divide often and quickly forming a lump. If the cancer cells can easily be transported the cancer can spread all over. The lump crowds out good cells and use up a lot of food and oxygen. Worksheet & Cancer Research 1. Explain a process in the human body in which there is evidence of the cell cycle at work. (1) Growth and development 2. Give two reasons why cells die. (2) Damaged Programmed to live a certain amount of time – no longer needed Lack of food or oxygen Failed mitosis 3. How do scientists currently explain the aging process? (2) The cell cycle is slowing down 4. Describe what happens when cells divide uncontrollably. (2) Cancer – these cells are like weeds in a garden, choking out the healthy plants/cells. 5. Using your knowledge about cancer and cell division, explain how you think sunscreen can help reduce the risk of skin cancer. (2) It blocks the UV rays and prevents them from damaging the genetic material 6. Some cells live for years, while others live for only a few days. Why do you think some cells might be replaced faster than others? (2) Some cells are damaged more quickly by their environments (stomach acid). 7. The muscle cells of the heart were once thought to stop dividing when a person reached the age of nine. Thus, heart attacks, which kill heart cells, were believed to cause permanent damage to the heart muscle. New research has discovered that mitosis does occur in the heart later in life. What do you think this new discovery means for people who have heart attacks? (2) Your body is capable of regenerating heart cells – this gives heart attack survivors hope for recovery What happens to our cells to bring about cancer? (2) Cancer is a disease that starts in our cells. Our bodies are made up of millions of cells, grouped together to form organs or tissues such as the lungs, the liver, muscles and bones. Genes inside each cell order it to grow, work, reproduce and die. What is a tumor/lump made up of? (2) Normally these orders are clear, our cells obey and we remain healthy. Sometimes a cell’s instructions get mixed up and it behaves abnormally. After a while groups of abnormal cells form lumps or tumours. What is the difference between a benign and malignant tumour? (2) Tumours can be either benign (noncancerous) or malignant (cancerous). Benign tumour cells stay in one place in the body and are not usually lifethreatening. Malignant tumour cells are able to invade the tissues around them and spread to other parts of the body. Cancerous cells that spread to other parts of the body are called metastases. The first sign that a malignant tumour has spread is often swelling of nearby lymph nodes, but cancer can metastasize to almost any part of the body. Malignant tumours can be dangerous. It is important to find them and treat them quickly, before they spread. How are cancers named? (2) Cancers are named after the part of the body where they start. For example, cancer that starts in the colon but spreads to the liver is called colon cancer with liver metastases. 1.4 Asexual Reproduction - Bacteria, Protists, Fungi, and Some Animals Asexual Reproduction – the formation of a new individual that has identical genetic information to its parent. Reproduction Asexual Sexual Binary Fission Budding Conjugation Spore Formation Fragmentation Hermaphroditic Vegetative Reproduction Separate Sexes Bacteria – see p 30 1. 2. 3. Have no nuclear membrane (prokaryotic) Have only one chromosome Reproduction asexually by the process of binary fission. 4. steps: a) b) c) d) e) cell wall ruptures single chromosome gets copied cell grows longer and a chromosome moves to each end. cell membrane pinches off. cell wall grows to surround both cells. Protists 1. 2. 3. Ex) amoeba, euglena, vorticella unicellular organisms with a true nucleus (eukaryotic). mitosis takes place creating two identical cells. Fungi 1. 2. Ex) Moulds, yeast, and mushrooms filaments called hyphae grow over the surface of food. 3. have 3 methods of asexual reproduction a) fragmentation – a small piece of hyphae breaks away and grows into a new individual. It is identical to its parent. b) • budding – a copy of the nucleus is made. The nuclei press against the cell membrane, forming a bud which grows and breaks off. (yeast) http://coralreefmultimed ia.org/reef/shorts.pop?t opic=Reproduction# c) spores – special cells called spores are stored in a case called a sporangium. The spores are released when ready and go through mitotic cell division if they land in suitable conditions. Animals Invertebrates (without backbone) make up 97% of animal species 2. Ex) Planaria (Flatworms) – divide in two and replace the parts that are missing. 3. Ex) Sponges and hydras – reproduce by budding. see pg 35. READ p 29-34 and do questions 1 – 6. 1. V. 1. 2. Asexual Reproduction in Plants Plants have an amazing ability to repair themselves using mitosis. They are often even able to regrow from a stump. Recall: asexual means DNA is exactly the same as its parent. Meristem 1. 2. 3. 4. animals stop growing at an adult size Plants grow until they die. Meristem – unspecialized cells in the stem and root tips which undergo mitosis very often. meristem cells divide and promote growth at the stem and root tips. Meristem 4. At some point some meristem cells specialize to do only a small range of functions. These cells no longer divide. 5. Meristem are active at stems and root tips, but can become active in other parts of the plant IF repairs to the plant are needed. Methods of Plant Asexual Reproduction Cloning 1. The process of making identical offspring to the parent using a single cell or a small amount of tissue from the parent. Humans use “cuttings” or pieces of a plant stem to place into soil and produce many offspring identical to the parent. 2. New Plants from Roots 3. these plants can grow from just root cuttings – pg 38 Growers can sell just the roots. Eg. Dandelions, asparagus New Plants from Stems These plants grow from just the tips of the runners – p 39 E.g. strawberries (runners) Layering – A branch of the parent plant is bent down to the ground covered with soil. Grafting 4. one part of a plant is cut and connected to a similar plant. The result is a plant that is part one type and part another. used for apples, grapes and roses. Tissue Cultures 5. take a few unspecialized cells from a plant place the cells in a special growing solution perfect for growth. the laboratory produces many identical plants (1 from each sample) useful for Chrysanthemums, orchids and pine trees. (Note – specialized cells have been altered to be able to carry out a few specialties very well but cannot carry out other functions.) See BLM 1-26, 28, 29,30, 31 and Do BLM 1-32 & 1-33 Grafting Grafting can add variety and bounty to apple orchards You have a fruit tree but you don't really like it. Despite proper pruning and tender care, you're getting poor apples or less-than-pretty pears. Maybe you hate the healthy fruit -- too sour, too squishy, too tart. Dormant sprays might not be working to rid you of pests. Well, don't cut down that tree just yet. Consider first the ultimate art of propagation -fruit tree grafting. Grafting -- how nearly all fruitful fruit trees are created nowadays -- means cutting a branch, large or small, off an undesirable tree and putting the limb of a new variety in its place. "Really, truly it's a lost art," Master Gardener Keith Underwood of Olympia said. "You take a pencil-size-around diameter twig and actually put it into the other bark of the tree." Most apple trees are, in fact, propagated by using strong root systems of other apple trees. "They're all in the apple family," said Underwood, who grafted thousands of trees during his days of apple farming near Yakima. So if you have an apple variety in your yard, but it's ugly because of scab, you might add on more scab-resistant varieties such as Chehalis or Liberty, or a more vigorous variety such as the Gravenstein. Try, eventually, up to 20 different varieties, depending on the tree size. Scott Stiles, a horticulturist at Raintree Nursery in Morton, said people love the nursery's workshops on grafting, which he'll be teaching in part next Saturday. "It's like magic, in a way," Stiles said. "You're creating a new tree from some simple little carpentry that never fails to fascinate people who watch it." Gardeners can take scion wood and replicate trees from a grandparent's old homestead. "They can just go cut a branch off that old heirloom tree," Stiles said, "and they can create them to pass along to their children. It's absolutely magic." "There's not a lot of people who do it anymore," Underwood said. "It's hard to find good grafters." Yet, Underwood does it on his farm in Olympia. He demonstrates the cuts for a 2-inch diameter stump on his Gravenstein, where he's injects three Spitzenburg apple tree twigs just under the bark. Each twig has two or three buds above the graft -- secured with nails and sealed with masking tape and a homemade wax of beeswax, Italian resin and linseed oil. "This is called a bark graft," Underwood says, "If you do this right, and you don't get a frost, every single one of them will take off." Underwood warns that gardeners borrowing from neighbors should beware of bringing diseases to their fruit trees. "The problem is viruses," Underwood said. "You have to be very aware." Scion wood -- the twiggy branches added to other trees -- can be purchased or gathered with permission from a neighbor's healthy tree. Using special techniques and a grafting knife, gardeners can add a different variety of fruit to their existing tree, and that graft can produce fruit within a few years. "This is just a tool that you can use to get more than one variety out of an apple tree," Underwood said. "I've taken 100-year-old trees and cut all the branches back and grafted every single stump."