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DO NOW: 1.) 2. 3. Things to remember when using a microscope 1. Carrying microscope always use one hand on arm and the other hand under base close to your body 2. DO NOT use the coarse adjustment under high power 3. Always start off with low power b/c it gives you a greater field of view. 4. Before switching to high power you must: a. focus your image b. center your image 5. When cleaning a lens, use LENS PAPER. Do not use a napkin, or paper towel… Unstained cheek cells Stained cheek cells Total magnification Mathematical calculation that tells you how much larger the specimen is than its actual size Formula : Total magnification = Eyepiece X Objective lens Total magnification=4 x10= 40 100x 400x Total Magnification: Ocular (Eyepiece) X Objective = Total magnification Practice: Eyepiece Objective Total lens magnification 20x 4x 25x 10x 15x 40x Image Appearance Enlarged Upside down & backwards On the slide Seen with naked eye F Seen through lens Aim: How do I prepare a Wet Mount Do Now: Identify 4 parts of scope 1. Place a drop of water on the slide 2. Place specimen on the water using tweezers. 3. Slowly lower the cover slip onto specimen on an angle to avoid AIR BUBBLES. Staining Treating a specimen with solutions of different colors to make cell parts more visible 3. Excess water should be absorbed with a paper towel. Examples: 1. Lugol’s Iodine Used for plant cells Onion Cells stained with Lugol’s Iodine 2. Methylene blue Used for animal cells Skin Cells stained with Methylene Blue Total Magnification: Ocular (Eyepiece) X Objective = Total magnification Practice: Eyepiece Objective Total lens magnification 10x 4x 10x 15x 10x 60x