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Parts of a Compound Light Microscope Mirror / Light • the light source for a microscope, typically located in the base of the microscope. • Most light microscopes use low voltage, halogen bulbs with continuous variable lighting control located within the base. A. Mirror / Light Coarse adjustment knob • used to focus the microscope • You use it to move the objective lenses toward or away from the specimen. C. Course adjustment knob Fine adjustment knob • used to focus the microscope • This is the knob used to fine tune the focus on the specimen. • It is also used to focus on various parts of the specimen. • Generally one uses the coarse focus first to get close then moves to the fine focus knob for fine tuning. B. Fine adjustment knob Eye piece • The eyepiece or Ocular is what you look through at the top of the microscope. • Most eyepieces have a magnifying power of 10x. D. Eye piece Nose piece • The part of the microscope that holds the objective lenses also called a revolving nosepiece or turret. • The objectives are exposed and are mounted so that different objectives can be selected. • Standard objectives include 4x, 10x, 40x and 100x. F. Nose Objective lenses • The primary optical lenses on a microscope. • They range from 4x-100x and typically, include, three, four or five on lens on most microscopes. G. Objective lenses Stage clips • Holds the slide in place • The viewer is required to move the slide manually to view different sections of the specimen. H. Stage Clips Stage • where the specimen to be viewed is placed. I. Stage Diaphragm • controls the amount of light reaching the specimen. J. Diaphragm Hooke's compound microscope • From "The Microscope and Its Revelations" Carpenter, 1891: image of Hooke's compound microscope 1665 Leeuwenhoek's simple microscope • From "The Microscope and Its Revelations" Carpenter, 1891: images of Leeuwenhoek's simple microscope Today we will be looking at: • • • • • • Salt crystals Wool fibers Cotton fibers The letter “e” Dust Volcanic ash colored thread newspaper print nylon fibers cork silk fibers corn starch • You will select 5 to practice focusing & drawing (in color) under all three powers D. Eye piece C. Course adjustment knob B. Fine adjustment knob E. Nose G. Objective lenses H. Stage Clips I. Stage A. Mirror / Light J. Diaphragm