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Human and Other Primate Cells and Tissues Human Source Material Blood and blood products Vaginal secretions Semen Amniotic fluid Unfixed tissues Cerebrospinal, synovial, pleural, pericardial and peritoneal fluids Cell cultures Saliva Urine Tears Sputum Feces Vomit Other excretions and secretions Second column not covered in Bloodborne Pathogen Standard, possibly not occupationally related. Human Source Material May transmit infectious agents Imperfect knowledge of infectious status Incubation period (asymptomatic) No test for every pathogen Most tissues and body fluids Bloodborne Pathogens (HBV, HCV, HIV, HTLV-1) Pathogens causing Malaria, Syphilis, Babesiosis, Brucellosis, Leptospirosis, Arboviral infections, Relapsing fever, Creutzfeldt-Jacob disease and viral hemorrhagic fever Cell Culture Risks Contaminating pathogenic agents natural (often zoonotic) or inadvertent ~20 LAIs from primary cultures in last 30 years e.g., Herpes B (CHV-1), prions Oncogenic potential could be oncogene or oncogenic agent e.g., HPV-18, MPMV genomes in HeLa cells Unexpected (adventitious) agents e.g., SIV, STLV, SV5 in primate cells, HHV-8 in BCBL-1 cells Hazardous chemicals added to culture medium Cell Culture under Bloodborne Pathogen Standard ABSA requested OSHA’s interpretation in 1994: Do human cell cultures fall under the Bloodborne Pathogen (BBP) Standard? Response: All primary human cell cultures (explants) and subsequent in vitro passages fall under the BBP Standard To be exempted from the BBP requirements, cell strains and lines must undergo testing and characterization (documented) for bloodborne pathogens (not just HBV, HCV and HIV) Cell Culture Safety Extend Universal/Standard Precautions to all human and animal cell cultures Consider working at BSL 2 (most work there already to protect the cell cultures) Handle all cultures in a biosafety cabinet If human origin and not demonstrated to be free of human bloodborne pathogens, adhere to requirements of the BBP Standard Wear PPE appropriate to human source material Summary Human Source Materials May be regulated Can be biohazardous Use Universal Precautions at all times Visible blood means increased risk Don’t consider “normal” source Human and Nonhuman Primate Cell Cultures Treat human cultures as possible biohazards Beware of non-human primate cells Beware of CNS, corneal, pituitary cells Some cells may be OK at BSL 1