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My Favorite Pathway: Glycolysis/Gluconeogenesis Pallavi Penumetcha 11/25/08 Glycolysis (RAST): Glycolysis Pathway Condensed: Example of How to Investigate One Enzyme Enzyme 3.1.3.11 was not highlighted in the CDD Results SEED pathway Pfam Results NCBI Results Enzymes not called by RAST annotation EC Number and Name Other Databases? Notes 3.1.3.11 (fructose bisphosphatase) Yes: SEED 2.7.1.11 (6phosphofructokinase) Yes: SEED and Manatee •5 calls between 2 databases (2 in SEED and 3 in Manatee) •2 sequences in SEED match with two in Manateecall different EC numbers -SEED: 2.7.1.11 -Manatee: 2.7.1.56 (1-phosphofructokinase) •1 sequence different in Manatee •2 sequence match: same results in CDD •Sequence in Manatee has the superfamily related to phosphofructokinases 1.2.1.12 (glyceraldehyde3-phosphate dehydrogenase, phosphorylating) Yes: All three •All three have same protein sequence •SEED calls the EC number 1.2.1.59 (glyceraldehyde3-phosphate dehydrogenase NADP+, phosphorylating) •Shows that the different databases have different criteria for calling a protein •Confirmed as fructose bisphosphotase in CDD, Pfam and NCBI Fructose and Mannose Metabolism: Pentose Phosphate Pathway