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NaBIS Nano Bio Integrated Systems Advanced Microtechnologies for Cytogenetic Analysis Dorota Kwasny 17.09.2012 Technical University of Denmark Department of Micro- and Nanotechnology DTU Nanotech NanoBioIntegrated Systems GroupNaBIS Nano Bio Integrated Systems Outline 1) Cytogenetics 2) Cytogenetic Analysis Nowadays 3) Miniaturization of cytogenetic analysis – – – – Cell culture device Metaphase chromosome spreading FISH on chip Translocations detection 4) Conclusions 5) Acknowledgements NaBIS Nano Bio Integrated Systems NaBIS Nano Bio Integrated Systems Smeets et al., 2004, Clinical Biochemistry Cytogenetics NaBIS Nano Bio Integrated Systems www.ghr.nlm.nih.gov Cytogenetics NaBIS Cytogenetics Nano Bio Integrated Systems http://www.dsmz.de Cancer Cytogenetics http://atlasgeneticsoncology.org Pre- and postnatal diagnosis • Down syndrome • Turner syndrome • Leukemias • Lymphomas • Solid tumors Cytogenetic Analysis Nowadays NaBIS Nano Bio Integrated Systems Kwasny et al., Biomedical Microdevices 2012, Advanced microtechnologies for FISH Cytogenetic Analysis Nowadays NaBIS Nano Bio Integrated Systems Kwasny et al., Biomedical Microdevices 2012, Advanced microtechnologies for FISH NaBIS Cell culture devices Nano Bio Integrated Systems Cells 0h Cells 72h Shah et al., Micromachines 2011 FISHprep Shah et al., Sensors and Actuators 2011, Microfluidic bioreactor NaBIS Metaphase chromosome spreading Nano Bio Integrated Systems Manual spreading Spreading device Manual Spreading Vedarethinam et al., Sensors and Actuators 2010, Metaphase FISH on chip Spreading device NaBIS Nano Bio Integrated Systems Jain et al., 2007 FISH on chip NaBIS FISH on chip Control Vedarethinam et al., Sensors and Actuators 2010, Metaphase FISH on chip Nano Bio Integrated Systems FISH on Chip Chromosome translocations detection on a CD assay Target DNA sequence with translocation Chr3 Chr9 Probe for Chr3 Probe for Chr9 Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS Nano Bio Integrated Systems Chromosome translocations detection on a CD assay Target DNA sequence with translocation Chr3 Chr9 Probe for Chr3 Probe for Chr9 Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS Nano Bio Integrated Systems Chromosome translocations detection on a CD assay Target DNA sequence with translocation Chr3 Chr9 Probe for Chr3 Probe for Chr9 Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS Nano Bio Integrated Systems Chromosome translocations detection on a CD assay Target DNA sequence with translocation Chr3 Chr9 Probe for Chr3 Probe for Chr9 Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS Nano Bio Integrated Systems Chromosome translocations detection on a CD assay Target DNA sequence with translocation Chr3 Chr9 Probe for Chr3 Probe for Chr9 Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS Nano Bio Integrated Systems Probes Immobilization 1st Hybridisation Brøgger et al., LoC 2012, Centrifugally driven microfluiodic disc for chromosome translocations NaBIS 2nd Hybridisation Nano Bio Integrated Systems Conclusions NaBIS Nano Bio Integrated Systems Acknowledgements Indumathi Vedarethinam Pranjul Shah Anna Line Brøgger Maria Dimaki Asli Silahtaroglu Zeynep Tumer Winnie E. Svendsen NaBIS Nano Bio Integrated Systems
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