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Download CENTENNIAL HONORS COLLEGE Western Illinois University Undergraduate Research Day 2016 Poster Presentation
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CENTENNIAL HONORS COLLEGE Western Illinois University Undergraduate Research Day 2016 PosterPresentation ActinobacteriaShowsPotentialAntibioticCapacitybyInhibitingPseudogymnoacsus destructans MatthewGooch FacultyMentor:AndreaPorras-Alfaro Biology Whitenosesyndrome(WNS)isafungalinfectionaffectingseveralbatspeciesalongtheEastcoastand theMidwestregionsasitexpandswestwardinNorthAmerica.ThecausativeagentofWNSisthefungus P. destructans which has infected and killed nearly 75% of bat populations since its discovery in 2006 BacteriawereisolatedfromthewingsandfursurfacesofWNS-freebatsinNewMexicoandArizonato studypotentialantagonismagainstWNS.Aninvitrobi-layerplatemethodwasusedtodeterminetheir potentialtoproduceantifungalcompoundstoinhibitthegrowthofP.destructans.Bacterialgrowthwas allowed on R2A media, which is limited in nutrients, to promote the production of secondary metabolites. An overlay of fungal media was poured followed by of the culture of P. destructans. We tested30isolateswithnotapparentinhibitionbutapreviousstudyshowedatleast5.7%showactivity against WNS. Understanding the antifungal potential of external microbiota will provide insights on potentialmechanismsofcontrolforthisdevastatingdisease.
