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Background • 2 of the 6 kingdoms encompass all prokaryotic cells – Have no nucleus – No membrane bound organelles – Circular DNA (plasmid) – Reproduce sexually and asexually – Examples: Archaebacteria Eubacteria • In 1970 Carl Woese and his team of scientists found out that not all bacteria was closely related in fact they needed to be divided into different groups. Bacteria and Archae. There are 3 domains that encompass all organisms. 1. Bacteria 2. Archaea 3. Eukarya 5 common characteristics used for comparison among living organisms: • • • • • Cell type – prokaryotic vs eukaryotic Cell wall – make-up Body type – unicellular vs. multicellular Nutrition – autotrophic vs. heterotrophic Reproduction – sexual vs. asexual The Domain Archaea • “ancient” bacteria • Some of the first archaebacteria were discovered in Yellowstone National Park’s hot springs and geysers. Basic Facts • They live in extreme environments – hot springs or salty lakes) and normal environments (like soil and ocean water). • All are unicellular • No peptidoglycan in their cell wall. • Extremophiles • The Cell Walls, Cell Membranes, and Ribosomal RNA are different from those of other BACTERIA. Extremophiles • Don’t need oxygen to survive • They can produce ATP (energy) from sunlight • They can survive enormous temperature extremes • They can survive high doses of radiation (radioactivity) • They can survive under rocks and in ocean floor vents deep below the ocean’s surface • They can tolerate huge pressure differences Archaebacteria Types Methanogens sewage Thermoacidophiles Hot springs Great salt lakes Extreme Halophiles Methanogens • They release methane (CH4) as a waste product • Live in anoxic conditions • Areas of water that lack dissolved oxygen – Wetlands, swamps • Digestive tract of animals or humans responsible for the gas emitted via belch Thermoacidophiles • Live in the dark • Live without oxygen • Like temperature and pH extremes – – – – Temps as high as 110ºC (230ºF) Temps as low as 1ºC Acidic pH 2 Alkali pH 9 • Chemoautotrophs Thermo = temperature Acidophil = acid loving Other thermoacidophiles like to live in hot springs or geysers. Hot springs are pools of hot water that have moved toward earth's surface. The source of their heat is the hot magma beneath and they can reach temperatures as high as 400 degrees Fahrenheit Black Smokers In extreme temperatures and pressures, this is where some thermoacidophiles like to live. http://www.nps.gov/archive/yell/oldfaithfulcam.htm Old Faithful erupts more frequently than any of the other big geysers. Its average interval between eruptions is about 91 minutes. An eruption lasts 1 1/2 to 5 minutes, expels 3,700 - 8,400 gallons of boiling water, and reaches heights of 106 - 184 feet. Halophiles • Can live in water with salt concentrations exceeding 15% – The ocean’s concentration is roughly 4% • Live in Great Salt Lake, Dead Sea and evaporated • Halo = salt phil = loving The Great Salt Lake in Utah The Great Salt Lake in Utah • Great Salt Lake is actually three to five times saltier than the ocean. • Every year, members of the salt industry extract about 2.5 million tons of sodium chloride from the lake. • The Great Salt Lake has no fish. Kingdom Eubacteria “true bacteria” • Bacteria and archaea have similar structures. – plasmid – flagellum – pili pili plasma membrance chromosome cell wall plasmid This diagram shows the typical structure of a prokaryote. Archaea and bacteria look very similar, although they have important molecular differences. flagellum • Bacteria commonly come in three forms. –rod-shaped, called bacilli – spherical, called cocci –spiral, called spirilla or spirochetes Lactobacilli: rod-shaped • Archaea have many shapes. Spirochaeta: spiral Enterococci: spherical Obtaining Energy • Most scientists classify bacteria based on how they obtain energy: Photosynthesizers- use sunlight to produce their own food/energy Chemoautotrophs- breakdown chemicals in the soils for food/energy Heterotrophs- eat/digest other organisms for food/energy REPRODUCTION 1. Asexual – binary fission (single cell splits into 2 identical new cells) 2. Sexual – conjugation – trading DNA conjugation bridge TEM; magnification • Gram staining identifies bacteria. GRAM NEGATIVE GRAM POSITIVE – stains polymer peptidoglycan – gram-positive stains purple, more peptidoglycan – gram-negative stains pink, less peptidoglycan Gram-negative bacteria have a thin layer of peptidoglycan and stain red. Gram-positive bacteria have a thicker peptidoglycan layer and stain purple. Prokaryotes provide nutrients to humans and other animals. • Prokaryotes live in digestive systems of animals. – make vitamins – break down food – fill niches Bacteria help ferment many foods – yogurt, cheese – pickles, sauerkraut – soy sauce, vinegar Prokaryotes play important roles in ecosystems. • Prokaryotes have many functions in ecosystems. – photosynthesize – recycle carbon, nitrogen, hydrogen, sulfur – fix nitrogen – Bacteria can be used in mines to breakdown the surrounding rock and leave behind the useful ore/metal. – Used to breakdown chemical/oil spills Bacteria are harmful in two ways: 1. #1 - Bacteria can metabolize their host by using different parts of the body as their food source. Tuberculosis is a less common bacterial infection that attacks the lungs of humans. Mycobacterium tuberculosis is the bacteria that uses the lung tissue as a food source. The warm, moist environment allows the bacteria to reproduce and populate the lungs. #2 - Bacteria cause disease by secreting chemical compounds called toxins into their environment. Humans are most affected when food is not properly prepared. Food poisoning is the common name given to people who get severely sick after eating something that wasn’t cooked well. • In 1928, Alexander Fleming noticed a fungus growing on a Petri plate that was growing bacteria. – He saw that the bacteria did not grow near the fungus. – He concluded that the fungus was secreting a substance that killed the bacteria. • He later called the secretion penicillin. – Penicillin is one of many medicines that help fight bacterial infections. Antibiotics