Download Biodiversity - Berkeley Cosmology Group

Microbial Diversity
Project leader: Daniela Goltsman
Jose Villanueva, Victoria Partida,
Katherine Cantarero
Table Of Contents. . .
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Introduction
Inoculation
Isolation
FISH
Results
Conclusion
Background
• Why are microorganisms important?
• How are microorganisms studied today?
– Aseptic technique
– Dyes
• What was the goal of our experiment?
– To study microbial diversity around our
environment
Diversity of life
Sampling Sites
• Our samples:
– Jose’s bathroom
– UC Berkeley’s Strawberry Creek
– Foothill Rec. Room Keyboard
– Katherine’s Dorm
• Why did we choose these samples?
Which location
do YOU think
is the diverse
or “dirtiest” ?
What is inoculation?
• The introduction of microorganisms into
any media.
• For us this process took about three days
to complete.
• We incubated at 37 00 C
Jose’s Bathroom
Katherine’s dorm
Foothill Rec. room keyboard
UC Berkeley’s Strawberry Creek
Isolation
• Taking one colony or “population” of one
type of microorganism and making those
populations reproduce and grow.
Isolate from Jose’s bathroom
Isolate from rec. room keyboard
UC Berkeley’s Strawberry Creek
What is FISH?
• FISH: Fluorescent In Situ Hybridization.
• This is a powerful technique that can be
used to evaluate and analyze the
presence of organisms in their community,
their phylogeny, morphology, and number
by targeting the 16S rRNA.
FISH probes
• What is a probe?
– Short sequence of DNA that hybridizes
(binds) to a segment of the 16S rRNA.
– Each probe carries a different color dye.
• Our dyes:
– 4 different probes 3 different colors
– Blue- Gama proteobacteria & Eukaryotic cells
– Green- Universal bacteria
– Red- Beta proteobacteria
Sample 1 LAPTOP. Green: bacteria, red:
beta-proteobacteria.
Sample 2: STRAWBERRY CREEK Green: universal
bacteria, red: beta-proteobacteria, soft blue: gamaproteobacteria, bright blue: eukaryotes.
Sample 3:KATHERINE’S ROOM Red:
beta-proteobacteria, blue: eukaryotes.
Sample 4. Green: universal bacteria,
blue: eukaryotes
Conclusion
In conclusion, we learned that:
– Microorganisms are EVERYWHERE!
– There are many kinds of bacteria that take on
many shapes and sizes.
– Keyboards and bathrooms are the most
diverse
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Thank you!