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Microbial Diversity Project leader: Daniela Goltsman Jose Villanueva, Victoria Partida, Katherine Cantarero Table Of Contents. . . • • • • • • Introduction Inoculation Isolation FISH Results Conclusion Background • Why are microorganisms important? • How are microorganisms studied today? – Aseptic technique – Dyes • What was the goal of our experiment? – To study microbial diversity around our environment Diversity of life Sampling Sites • Our samples: – Jose’s bathroom – UC Berkeley’s Strawberry Creek – Foothill Rec. Room Keyboard – Katherine’s Dorm • Why did we choose these samples? Which location do YOU think is the diverse or “dirtiest” ? What is inoculation? • The introduction of microorganisms into any media. • For us this process took about three days to complete. • We incubated at 37 00 C Jose’s Bathroom Katherine’s dorm Foothill Rec. room keyboard UC Berkeley’s Strawberry Creek Isolation • Taking one colony or “population” of one type of microorganism and making those populations reproduce and grow. Isolate from Jose’s bathroom Isolate from rec. room keyboard UC Berkeley’s Strawberry Creek What is FISH? • FISH: Fluorescent In Situ Hybridization. • This is a powerful technique that can be used to evaluate and analyze the presence of organisms in their community, their phylogeny, morphology, and number by targeting the 16S rRNA. FISH probes • What is a probe? – Short sequence of DNA that hybridizes (binds) to a segment of the 16S rRNA. – Each probe carries a different color dye. • Our dyes: – 4 different probes 3 different colors – Blue- Gama proteobacteria & Eukaryotic cells – Green- Universal bacteria – Red- Beta proteobacteria Sample 1 LAPTOP. Green: bacteria, red: beta-proteobacteria. Sample 2: STRAWBERRY CREEK Green: universal bacteria, red: beta-proteobacteria, soft blue: gamaproteobacteria, bright blue: eukaryotes. Sample 3:KATHERINE’S ROOM Red: beta-proteobacteria, blue: eukaryotes. Sample 4. Green: universal bacteria, blue: eukaryotes Conclusion In conclusion, we learned that: – Microorganisms are EVERYWHERE! – There are many kinds of bacteria that take on many shapes and sizes. – Keyboards and bathrooms are the most diverse – Thank you!
