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Practical Review Practice( SemI) Advanced Placement Biology Laboratory Review Sheet Dissolved oxygen simulation http://www.phschool.com/science/biology_place/labbench/la b12/varlight.html Play the simulation. Relate to an aquatic ecosystem How does dissolved oxygen relate to primary productivity How do the following variables affect dissolved oxygen Temperature Salt water and fresh water? Running and still Question and answers If the water temperature increases, the dissolved oxygen________ Decreases In general running water has ________ ( more or less) oxygen than still water More Nomogram Read the % saturation For a temperature of 25oC, what is the % saturation ? For a temperature of 25oC, what is the dissolved oxygen? Class Microscope. http://www.microscope-microscope.org/basic/microscope-parts.htm Identify the following Objective lenses Condenser Mechanical stage contraol Fine Adjustment Coarse Adjustment Rotating noewpiece Be able to identify:the following Oil High Low Scanning How to prepare a wet mount. What are these steps? What is this cell? Starfish egg cell Is this cell a eukaryote or a prokaryote? What s the difference between these two pictures of Spirogyra? How would Spirogyra be classified? What is Volvox? Why does this show evolution? Spectrophotometer http://www.umd.umich.edu/casl/natsci/slc/spec21.swf - simulation Spectrophotometer What is absorbance? What is percent transmittance? What colors are associated with the wavelengths of visible light Chromatography with a Flair Chromatography What is the matrix? What is the solute? What is the solvent? What is the basis of separation? What is the Rf value? Gel electropohoresis What is an electrophoresis chamber ? Describe how to put the sample in the wells? The sample is loaded at this end of the gel box This shows the molecules moving toward the DNA gel What is the stain in the picture? What is the marker used with this experiment? DNA Electrophoresis Which is the marker? Micromeasurement What is the reading on this micropipet? Micropipet Use( review) Review how to use the micropipet http://www.fhcrc.org/education/hutchlab/lessons/use.html What is an isolation streak? What is flaming? What is the shape of these bacteria? Which one is gram positive? What type of bacteria are these? What type of bacteria are these? What type of bacteria are these? Kirby Bauer What is resistance , what is sensitivity?