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Susceptibility of Antibacterial Chemicals on Pseudomonas aeruginosa By Clayton Brady Health Science Biology Major Tennessee Tech University Project Summary Pseudomonas aeruginosa is a resistant bacteria that is difficult to kill in an infected human. Many antibacterial chemicals have been created with the task of disinfecting areas contaminated with this bacteria. Through the use of certain antibacterial agents such as Lysol, bleach, and Pine-sol, I am going to see if These household products we use every day are as effective as advertised. I expect that after testing Pseudomonas against these chemicals, that the bacteria will be more susceptible to the bleach than the other manufactured antibacterial chemicals. Introduction Strains of Pseudomonas aeruginosa from a synthetic stock will be tested against a test commonly used in microbiology laboratories. All mediums will be collected and examined to determine if Pseudomonas aeruginosa is susceptible to this test. The objective of my research is to determine if the antibacterial sprays we use so abundantly today can in fact render potentially hazardous bacteria, such as Pseudomonas aeruginosa, non-viable. By doing this I hope to find how safe we are using chemicals and sprays that we trust in our homes to keep us healthy. Pseudomonas Facts By transferring their genetic material from cell to cell, Pseudomonas aeruginosa is able to elude antimicrobial agents and antibiotics to survive. Thus making it a perfect "super subject" to test chemicals that claim to kill harmful bacteria. Pseudomonas aeruginosa is an opportunistic pathogen. This gram-negative bacterium can produce a large number of toxic proteins that can cause extensive tissue damage and also interfere with a human’s immune system. Pseudomonas aeruginosa also causes diseases such as dermatitis, urinary tract infections, and even respiratory infections such as pneumonia. Objective Statement The objective of my research is to determine if the antibacterial sprays we use so abundantly today can in fact render potentially hazardous bacteria, such as Pseudomonas aeruginosa, non-viable. Null Hypothesis I believe that the antibacterial chemicals will have no effect on Pseudomonas aeruginosa. I will disprove the Null hypothesis by proving that Pseudomonas aeruginosa is susceptible to certain antibacterial chemicals. Da t a Fo r m le Antibacterial Agent Bleach (5% hypochlorite solution) Pine-sol Lysol S t ib p ce us l ca i m ib he o) h C n fI ve or n i o t s ec ne Zo Eff (y e n it io Re nt a t sis Materials to be Used Pseudomonas aeruginosa synthetic stock or isolate from a biofilm. Petri dishes Bleach (5% hypochlorite solution) Pine-sol Lysol Agar solution for testing. Expected Results and Benefits I expect that, after careful aseptic experimentation, I will be able to kill Pseudomonas aeruginosa with a the antibacterial chemicals that will be used in the experimental process. Benefits of this experiment will include knowledge of bacterial protection for home and workplace use as well as a better understanding of this bacteria. Project Timelines The entire experimentation will take approximately 3 days to complete. 1st Day: Preparation of all agar plates and apparatuses. Streaking of plates. Incubation. 2nd Day: This day will be used to allow the bacteria to grow. 3rd Day: Determination of susceptibility and resistance of the bacteria to individual chemicals as well as pictures of the apparatuses. Budget All supplies are being provided by Dr. Goss in the microbiology/bacteriology laboratory at Tennessee Technological University. Home