Download Pseudomonas aeruginosa

Susceptibility of
Antibacterial Chemicals
on
Pseudomonas aeruginosa
By Clayton Brady
Health Science Biology Major
Tennessee Tech University
Project Summary
 Pseudomonas aeruginosa is a resistant bacteria that
is difficult to kill in an infected human.
 Many antibacterial chemicals have been created with
the task of disinfecting areas contaminated with this
bacteria.
 Through the use of certain antibacterial agents such
as Lysol, bleach, and Pine-sol, I am going to see if
These household products we use every day are as
effective as advertised.
 I expect that after testing Pseudomonas against these
chemicals, that the bacteria will be more susceptible to
the bleach than the other manufactured antibacterial
chemicals.
Introduction
Strains of Pseudomonas aeruginosa from a synthetic
stock will be tested against a test commonly used in
microbiology laboratories. All mediums will be
collected and examined to determine if Pseudomonas
aeruginosa is susceptible to this test. The objective of
my research is to determine if the antibacterial sprays
we use so abundantly today can in fact render
potentially hazardous bacteria, such as Pseudomonas
aeruginosa, non-viable. By doing this I hope to find
how safe we are using chemicals and sprays that we
trust in our homes to keep us healthy.
Pseudomonas Facts
By transferring their genetic material from cell to cell,
Pseudomonas aeruginosa is able to elude antimicrobial
agents and antibiotics to survive. Thus making it a
perfect "super subject" to test chemicals that claim to kill
harmful bacteria.
Pseudomonas aeruginosa is an opportunistic pathogen.
This gram-negative bacterium can produce a large
number of toxic proteins that can cause extensive tissue
damage and also interfere with a human’s immune
system.
Pseudomonas aeruginosa also causes diseases such as
dermatitis, urinary tract infections, and even respiratory
infections such as pneumonia.
Objective Statement
The objective of my research is to
determine if the antibacterial sprays we use
so abundantly today can in fact render
potentially hazardous bacteria, such as
Pseudomonas aeruginosa, non-viable.
Null Hypothesis
 I believe that the antibacterial
chemicals will have no effect on
Pseudomonas aeruginosa.
 I will disprove the Null hypothesis by
proving that Pseudomonas aeruginosa
is susceptible to certain antibacterial
chemicals.
Da t a Fo r m
le
Antibacterial Agent
Bleach (5% hypochlorite solution)
Pine-sol
Lysol
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Materials to be Used
 Pseudomonas aeruginosa synthetic
stock or isolate from a biofilm.
 Petri dishes
 Bleach (5% hypochlorite solution)
 Pine-sol
 Lysol
 Agar solution for testing.
Expected Results and
Benefits
 I expect that, after careful aseptic
experimentation, I will be able to kill
Pseudomonas aeruginosa with a the
antibacterial chemicals that will be used
in the experimental process.
 Benefits of this experiment will include
knowledge of bacterial protection for
home and workplace use as well as a
better understanding of this bacteria.
Project Timelines
 The entire experimentation will take
approximately 3 days to complete.
 1st Day: Preparation of all agar plates and
apparatuses. Streaking of plates. Incubation.
 2nd Day: This day will be used to allow the
bacteria to grow.
 3rd Day: Determination of susceptibility and
resistance of the bacteria to individual
chemicals as well as pictures of the
apparatuses.
Budget
All supplies are being provided by Dr.
Goss in the microbiology/bacteriology
laboratory at Tennessee Technological
University.
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