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catalyst 1. In what ways do CSI and police investigators “catch the bad guys?” What scientific evidence do they need? 2. Have you ever witnessed a crime scene investigation? Unit 5 Test Biotechnology • 5 “stations” • 3:30 minutes each • Fill in your notes! Biotechnology • https://www.youtube.com/watch?v=Wm 6_BBS_KE4 Human Genome Project • https://www.youtube.com/watch?v=N0QZDOcqyO4 Gene Therapy https://www.youtube.com/watch?v=H 0RvTOF1fEc Stem Cell Research • https://www.youtube.com/watch?v=tPul EAryPO0 Cloning • https://www.youtube.com/watch?v=E oqBhtwgPmw Gel electrophoresis https://www.youtube.com/watch?v=WwgsFjvWlw Purpose order nitrogenous bases fragments completely size How? cut cut DNA into fragments using 1. ________: ______________ _____________ restriction enzymes(molecular scissors!) load put the DNA fragments into 2. __________: wells of a gel run ____: it! connect a power source. 3. _______ __________________ negatively charged DNA moves to the positive (+) end! jello i. “swimming through __________” ii. Larger fragments move slower iii. _______________ fragments move faster (& farther!) smaller How? read____: it! interpret the results! 4. _______ Which suspect was at the crime scene? Gel electrophoresis Uses? paternity catalyst 1.What type of enzyme acts like “molecular scissors” to cut DNA? 2.List the 5 biotechnologies that we learned about yesterday DNA different transgenic bacteria plasmid proteins traits PROBLEM: People have diabetes and cannot make insulin SOLUTION: use recombinant DNA to force bacteria to make insulin for us! https://www.youtube.com/watch?v=8rX izmLjegI plasmid plasmid restriction plasmid restriction lipase synthesizes asexually plasmid human DNA Recombinant DNA Lab READ CAREFULLY •Working with your partner •Once you have read ALL of the instructions through one time, you may raise your hand for materials Practice Pg U5-12 & U5-13 catalyst 1. If you could clone (make a copy) of any LIVING thing – what would you clone? Why? 2. If you could pick your baby’s characteristics, would you?