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化脓菌分离鉴定过程(三)
化脓菌分离鉴定过程
葡萄球菌
• 甘露醇发酵试验
• 血浆凝固酶试验
• 耐热核酸酶试验
• 动物试验
甘露醇发酵试验
Inoculate the bacteria into a mannitol
micro-tube
Incubate at 37℃ for 18h.
S.aureus will ferment mannitol to
produce acid, which causes the medium
to turn yellow.
血浆凝固酶试验(The Coagulase Test)
 Coagulase
• 结合凝固酶
• 游离凝固酶
• 凝固酶
协同因子
纤维蛋白原
凝血酶样物质
纤维蛋白
 It has been speculated that this enzyme might be a
virulence factor. However, coagulase-negative
strains are often as pathogenic as coagulasepositive strains.
 There are two ways of performing this test
• The Slide Coagulase Test
• The Tube coagulase Test
Tube coagulase Test
+
-
The Slide Coagulase Test
细菌的药物敏感性试验
纸片扩散法(K-B法)
肠道菌分离鉴定过程(三)
Antigens of salmonella
O antigen
H antigen
Vi antigen
polysaccharide of LPS
used to divide Salmonella to 42 groups
(A-Z,O51-63,O65-67)
A-E groups are pathogenic
flagella antigen
divide Salmonella tow phase:
special no special
related to the virulence of Salmonella
sensitive to heat ( lose activity at 60℃)
Serological Identification of Salmonella
Select the specimen
React with A-Z polyvalent antiserum
Agglutination
reaction
(+)
React with several individual
types of anti-O and anti-H
antiserum
Identify its group and phage
(-)
React with anti-Vi
antiserum
系统实验报告(报告三)
化脓菌/肠道菌分离鉴定过程
目的
策略(原理设计)
方法步骤:简单写
结果(判定方法)
结论